                         SEQUENCE LISTING

<110>  ALSIA - Centro Ricerche Metapontum Agrobios
 
<120>  Allelic variant of the Cyc-B gene for the constitution of tomato 
       varieties resistant to broomrape

<130>  P1188PC

<140>  MI2015A000009
<141>  2015-01-09

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  External forward primer F1

<400>  1
gctcttctca agccttttcc                                                   20


<210>  2
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  External reverse primer R1

<400>  2
gccaatcagt ctaaccaaag g                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nested forward primer F2

<400>  3
cctctcctac accccatagg t                                                 21


<210>  4
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nested reverse primer R2

<400>  4
agccatgtcc gaaaagacac                                                   20


<210>  5
<211>  1497
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant Cyc-B gene


<220>
<221>  modified_base
<222>  (949)..(949)
<223>  Guanine in place of Adenine

       Allelic variant of lycopene cyclase Cyc B

<400>  5
atggaagctc ttctcaagcc ttttccatct cttttacttt cctctcctac accccatagg       60

tctattttcc aacaaaatcc ctcttttcta agtcccacca ccaaaaaaaa atcaagaaaa      120

tgtcttctta gaaacaaaag tagtaaactt ttttgtagct ttcttgattt agcacccaca      180

tcaaagccag agtctttaga tgttaacatc tcatgggttg atcctaattc gaatcgggct      240

caattcgacg tgatcattat cggagctggc cctgctgggc tcaggctagc tgaacaagtt      300

tctaaatatg gtattaaggt atgttgtgtt gacccttcac cactctccat gtggccaaat      360

aattatggtg tttgggttga tgagtttgag aatttaggac tggaaaattg tttagatcat      420

aaatggccta tgacttgtgt gcatataaat gataacaaaa ctaagtattt gggaagacca      480

tatggtagag ttagtagaaa gaagctgaag ttgaaattgt tgaatagttg tgttgagaac      540

agagtgaagt tttataaagc taaggtttgg aaagtggaac atgaagaatt tgagtcttca      600

attgtttgtg atgatggtaa gaagataaga ggtagtttgg ttgtggatgc aagtggtttt      660

gctagtgatt ttatagagta tgacaggcca agaaaccatg gttatcaaat tgctcatggg      720

gttttagtag aagttgataa tcatccattt gatttggata aaatggtgct tatggattgg      780

agggattctc atttgggtaa tgagccatat ttaagggtga ataatgctaa agaaccaaca      840

ttcttgtatg caatgccatt tgatagagat ttggttttct tggaagagac ttctttggtg      900

agtcgtcctg ttttatcgta tatggaagta aaaagaagga tggtggcagg attaaggcat      960

ttggggatca aagtgaaaag tgttattgag gaagagaaat gtgtgatccc tatgggagga     1020

ccacttccgc ggattcctca aaatgttatg gctattggtg ggaattcagg gatagttcat     1080

ccatcaacag ggtacatggt ggctaggagc atggctttag caccagtact agctgaagcc     1140

atcgtcgagg ggcttggctc aacaagaatg ataagagggt ctcaacttta ccatagagtt     1200

tggaatggtt tgtggccttt ggatagaaga tgtgttagag aatgttattc atttgggatg     1260

gagacattgt tgaagcttga tttgaaaggg actaggagat tgtttgacgc tttctttgat     1320

cttgatccta aatactggca agggttcctt tcttcaagat tgtctgtcaa agaacttggt     1380

ttactcagct tgtgtctttt cggacatggc tcaaacatga ctaggttgga tattgttaca     1440

aaatgtcctc ttcctttggt tagactgatt ggcaatctag caatagagag cctttga        1497


<210>  6
<211>  1497
<212>  DNA
<213>  Solanum lycopersicum


<220>
<221>  exon
<222>  (1)..(1497)
<223>  Wild type nucleotide sequence of lycopene cyclase Cyc B

<400>  6
atg gaa gct ctt ctc aag cct ttt cca tct ctt tta ctt tcc tct cct         48
Met Glu Ala Leu Leu Lys Pro Phe Pro Ser Leu Leu Leu Ser Ser Pro           
1               5                   10                  15                

aca ccc cat agg tct att ttc caa caa aat ccc tct ttt cta agt ccc         96
Thr Pro His Arg Ser Ile Phe Gln Gln Asn Pro Ser Phe Leu Ser Pro           
            20                  25                  30                    

acc acc aaa aaa aaa tca aga aaa tgt ctt ctt aga aac aaa agt agt        144
Thr Thr Lys Lys Lys Ser Arg Lys Cys Leu Leu Arg Asn Lys Ser Ser           
        35                  40                  45                        

aaa ctt ttt tgt agc ttt ctt gat tta gca ccc aca tca aag cca gag        192
Lys Leu Phe Cys Ser Phe Leu Asp Leu Ala Pro Thr Ser Lys Pro Glu           
    50                  55                  60                            

tct tta gat gtt aac atc tca tgg gtt gat cct aat tcg aat cgg gct        240
Ser Leu Asp Val Asn Ile Ser Trp Val Asp Pro Asn Ser Asn Arg Ala           
65                  70                  75                  80            

caa ttc gac gtg atc att atc gga gct ggc cct gct ggg ctc agg cta        288
Gln Phe Asp Val Ile Ile Ile Gly Ala Gly Pro Ala Gly Leu Arg Leu           
                85                  90                  95                

gct gaa caa gtt tct aaa tat ggt att aag gta tgt tgt gtt gac cct        336
Ala Glu Gln Val Ser Lys Tyr Gly Ile Lys Val Cys Cys Val Asp Pro           
            100                 105                 110                   

tca cca ctc tcc atg tgg cca aat aat tat ggt gtt tgg gtt gat gag        384
Ser Pro Leu Ser Met Trp Pro Asn Asn Tyr Gly Val Trp Val Asp Glu           
        115                 120                 125                       

ttt gag aat tta gga ctg gaa aat tgt tta gat cat aaa tgg cct atg        432
Phe Glu Asn Leu Gly Leu Glu Asn Cys Leu Asp His Lys Trp Pro Met           
    130                 135                 140                           

act tgt gtg cat ata aat gat aac aaa act aag tat ttg gga aga cca        480
Thr Cys Val His Ile Asn Asp Asn Lys Thr Lys Tyr Leu Gly Arg Pro           
145                 150                 155                 160           

tat ggt aga gtt agt aga aag aag ctg aag ttg aaa ttg ttg aat agt        528
Tyr Gly Arg Val Ser Arg Lys Lys Leu Lys Leu Lys Leu Leu Asn Ser           
                165                 170                 175               

tgt gtt gag aac aga gtg aag ttt tat aaa gct aag gtt tgg aaa gtg        576
Cys Val Glu Asn Arg Val Lys Phe Tyr Lys Ala Lys Val Trp Lys Val           
            180                 185                 190                   

gaa cat gaa gaa ttt gag tct tca att gtt tgt gat gat ggt aag aag        624
Glu His Glu Glu Phe Glu Ser Ser Ile Val Cys Asp Asp Gly Lys Lys           
        195                 200                 205                       

ata aga ggt agt ttg gtt gtg gat gca agt ggt ttt gct agt gat ttt        672
Ile Arg Gly Ser Leu Val Val Asp Ala Ser Gly Phe Ala Ser Asp Phe           
    210                 215                 220                           

ata gag tat gac agg cca aga aac cat ggt tat caa att gct cat ggg        720
Ile Glu Tyr Asp Arg Pro Arg Asn His Gly Tyr Gln Ile Ala His Gly           
225                 230                 235                 240           

gtt tta gta gaa gtt gat aat cat cca ttt gat ttg gat aaa atg gtg        768
Val Leu Val Glu Val Asp Asn His Pro Phe Asp Leu Asp Lys Met Val           
                245                 250                 255               

ctt atg gat tgg agg gat tct cat ttg ggt aat gag cca tat tta agg        816
Leu Met Asp Trp Arg Asp Ser His Leu Gly Asn Glu Pro Tyr Leu Arg           
            260                 265                 270                   

gtg aat aat gct aaa gaa cca aca ttc ttg tat gca atg cca ttt gat        864
Val Asn Asn Ala Lys Glu Pro Thr Phe Leu Tyr Ala Met Pro Phe Asp           
        275                 280                 285                       

aga gat ttg gtt ttc ttg gaa gag act tct ttg gtg agt cgt cct gtt        912
Arg Asp Leu Val Phe Leu Glu Glu Thr Ser Leu Val Ser Arg Pro Val           
    290                 295                 300                           

tta tcg tat atg gaa gta aaa aga agg atg gtg gca aga tta agg cat        960
Leu Ser Tyr Met Glu Val Lys Arg Arg Met Val Ala Arg Leu Arg His           
305                 310                 315                 320           

ttg ggg atc aaa gtg aaa agt gtt att gag gaa gag aaa tgt gtg atc       1008
Leu Gly Ile Lys Val Lys Ser Val Ile Glu Glu Glu Lys Cys Val Ile           
                325                 330                 335               

cct atg gga gga cca ctt ccg cgg att cct caa aat gtt atg gct att       1056
Pro Met Gly Gly Pro Leu Pro Arg Ile Pro Gln Asn Val Met Ala Ile           
            340                 345                 350                   

ggt ggg aat tca ggg ata gtt cat cca tca aca ggg tac atg gtg gct       1104
Gly Gly Asn Ser Gly Ile Val His Pro Ser Thr Gly Tyr Met Val Ala           
        355                 360                 365                       

agg agc atg gct tta gca cca gta cta gct gaa gcc atc gtc gag ggg       1152
Arg Ser Met Ala Leu Ala Pro Val Leu Ala Glu Ala Ile Val Glu Gly           
    370                 375                 380                           

ctt ggc tca aca aga atg ata aga ggg tct caa ctt tac cat aga gtt       1200
Leu Gly Ser Thr Arg Met Ile Arg Gly Ser Gln Leu Tyr His Arg Val           
385                 390                 395                 400           

tgg aat ggt ttg tgg cct ttg gat aga aga tgt gtt aga gaa tgt tat       1248
Trp Asn Gly Leu Trp Pro Leu Asp Arg Arg Cys Val Arg Glu Cys Tyr           
                405                 410                 415               

tca ttt ggg atg gag aca ttg ttg aag ctt gat ttg aaa ggg act agg       1296
Ser Phe Gly Met Glu Thr Leu Leu Lys Leu Asp Leu Lys Gly Thr Arg           
            420                 425                 430                   

aga ttg ttt gac gct ttc ttt gat ctt gat cct aaa tac tgg caa ggg       1344
Arg Leu Phe Asp Ala Phe Phe Asp Leu Asp Pro Lys Tyr Trp Gln Gly           
        435                 440                 445                       

ttc ctt tct tca aga ttg tct gtc aaa gaa ctt ggt tta ctc agc ttg       1392
Phe Leu Ser Ser Arg Leu Ser Val Lys Glu Leu Gly Leu Leu Ser Leu           
    450                 455                 460                           

tgt ctt ttc gga cat ggc tca aac atg act agg ttg gat att gtt aca       1440
Cys Leu Phe Gly His Gly Ser Asn Met Thr Arg Leu Asp Ile Val Thr           
465                 470                 475                 480           

aaa tgt cct ctt cct ttg gtt aga ctg att ggc aat cta gca ata gag       1488
Lys Cys Pro Leu Pro Leu Val Arg Leu Ile Gly Asn Leu Ala Ile Glu           
                485                 490                 495               

agc ctt tga                                                           1497
Ser Leu                                                                   
                                                                          


<210>  7
<211>  498
<212>  PRT
<213>  Solanum lycopersicum


<220>
<221>  MISC-FEATURE
<222>  (1)..(498)
<223>  Wild type aminoacid sequence of lycopene cyclase Cyc-b

<400>  7

Met Glu Ala Leu Leu Lys Pro Phe Pro Ser Leu Leu Leu Ser Ser Pro 
1               5                   10                  15      


Thr Pro His Arg Ser Ile Phe Gln Gln Asn Pro Ser Phe Leu Ser Pro 
            20                  25                  30          


Thr Thr Lys Lys Lys Ser Arg Lys Cys Leu Leu Arg Asn Lys Ser Ser 
        35                  40                  45              


Lys Leu Phe Cys Ser Phe Leu Asp Leu Ala Pro Thr Ser Lys Pro Glu 
    50                  55                  60                  


Ser Leu Asp Val Asn Ile Ser Trp Val Asp Pro Asn Ser Asn Arg Ala 
65                  70                  75                  80  


Gln Phe Asp Val Ile Ile Ile Gly Ala Gly Pro Ala Gly Leu Arg Leu 
                85                  90                  95      


Ala Glu Gln Val Ser Lys Tyr Gly Ile Lys Val Cys Cys Val Asp Pro 
            100                 105                 110         


Ser Pro Leu Ser Met Trp Pro Asn Asn Tyr Gly Val Trp Val Asp Glu 
        115                 120                 125             


Phe Glu Asn Leu Gly Leu Glu Asn Cys Leu Asp His Lys Trp Pro Met 
    130                 135                 140                 


Thr Cys Val His Ile Asn Asp Asn Lys Thr Lys Tyr Leu Gly Arg Pro 
145                 150                 155                 160 


Tyr Gly Arg Val Ser Arg Lys Lys Leu Lys Leu Lys Leu Leu Asn Ser 
                165                 170                 175     


Cys Val Glu Asn Arg Val Lys Phe Tyr Lys Ala Lys Val Trp Lys Val 
            180                 185                 190         


Glu His Glu Glu Phe Glu Ser Ser Ile Val Cys Asp Asp Gly Lys Lys 
        195                 200                 205             


Ile Arg Gly Ser Leu Val Val Asp Ala Ser Gly Phe Ala Ser Asp Phe 
    210                 215                 220                 


Ile Glu Tyr Asp Arg Pro Arg Asn His Gly Tyr Gln Ile Ala His Gly 
225                 230                 235                 240 


Val Leu Val Glu Val Asp Asn His Pro Phe Asp Leu Asp Lys Met Val 
                245                 250                 255     


Leu Met Asp Trp Arg Asp Ser His Leu Gly Asn Glu Pro Tyr Leu Arg 
            260                 265                 270         


Val Asn Asn Ala Lys Glu Pro Thr Phe Leu Tyr Ala Met Pro Phe Asp 
        275                 280                 285             


Arg Asp Leu Val Phe Leu Glu Glu Thr Ser Leu Val Ser Arg Pro Val 
    290                 295                 300                 


Leu Ser Tyr Met Glu Val Lys Arg Arg Met Val Ala Arg Leu Arg His 
305                 310                 315                 320 


Leu Gly Ile Lys Val Lys Ser Val Ile Glu Glu Glu Lys Cys Val Ile 
                325                 330                 335     


Pro Met Gly Gly Pro Leu Pro Arg Ile Pro Gln Asn Val Met Ala Ile 
            340                 345                 350         


Gly Gly Asn Ser Gly Ile Val His Pro Ser Thr Gly Tyr Met Val Ala 
        355                 360                 365             


Arg Ser Met Ala Leu Ala Pro Val Leu Ala Glu Ala Ile Val Glu Gly 
    370                 375                 380                 


Leu Gly Ser Thr Arg Met Ile Arg Gly Ser Gln Leu Tyr His Arg Val 
385                 390                 395                 400 


Trp Asn Gly Leu Trp Pro Leu Asp Arg Arg Cys Val Arg Glu Cys Tyr 
                405                 410                 415     


Ser Phe Gly Met Glu Thr Leu Leu Lys Leu Asp Leu Lys Gly Thr Arg 
            420                 425                 430         


Arg Leu Phe Asp Ala Phe Phe Asp Leu Asp Pro Lys Tyr Trp Gln Gly 
        435                 440                 445             


Phe Leu Ser Ser Arg Leu Ser Val Lys Glu Leu Gly Leu Leu Ser Leu 
    450                 455                 460                 


Cys Leu Phe Gly His Gly Ser Asn Met Thr Arg Leu Asp Ile Val Thr 
465                 470                 475                 480 


Lys Cys Pro Leu Pro Leu Val Arg Leu Ile Gly Asn Leu Ala Ile Glu 
                485                 490                 495     


Ser Leu 
        


<210>  8
<211>  1503
<212>  DNA
<213>  Solanum lycopersicum


<220>
<221>  exon
<222>  (1)..(1503)
<223>  Wild type nucleotide sequence of lycopene cyclase Lcy B

<400>  8
atg gat act ttg ttg aaa acc cca aat aac ctt gaa ttt ctg aac cca         48
Met Asp Thr Leu Leu Lys Thr Pro Asn Asn Leu Glu Phe Leu Asn Pro           
1               5                   10                  15                

cat cat ggt ttt gct gtt aaa gct agt acc ttt aga tct gag aag cat         96
His His Gly Phe Ala Val Lys Ala Ser Thr Phe Arg Ser Glu Lys His           
            20                  25                  30                    

cat aat ttt ggt tct agg aag ttt tgt gaa act ttg ggt aga agt gtt        144
His Asn Phe Gly Ser Arg Lys Phe Cys Glu Thr Leu Gly Arg Ser Val           
        35                  40                  45                        

tgt gtt aag ggt agt agt agt gct ctt tta gag ctt gta cct gag acc        192
Cys Val Lys Gly Ser Ser Ser Ala Leu Leu Glu Leu Val Pro Glu Thr           
    50                  55                  60                            

aaa aag gag aat ctt gat ttt gag ctt cct atg tat gac cct tca aaa        240
Lys Lys Glu Asn Leu Asp Phe Glu Leu Pro Met Tyr Asp Pro Ser Lys           
65                  70                  75                  80            

ggg gtt gtt gtg gat ctt gct gtg gtt ggt ggt ggc cct gca gga ctt        288
Gly Val Val Val Asp Leu Ala Val Val Gly Gly Gly Pro Ala Gly Leu           
                85                  90                  95                

gct gtt gca cag caa gtt tct gaa gca gga ctc tct gtt tgt tca att        336
Ala Val Ala Gln Gln Val Ser Glu Ala Gly Leu Ser Val Cys Ser Ile           
            100                 105                 110                   

gat ccg aat cct aaa ttg ata tgg cct aat aac tat ggt gtt tgg gtg        384
Asp Pro Asn Pro Lys Leu Ile Trp Pro Asn Asn Tyr Gly Val Trp Val           
        115                 120                 125                       

gat gaa ttt gag gct atg gac ttg tta gat tgt cta gat gct acc tgg        432
Asp Glu Phe Glu Ala Met Asp Leu Leu Asp Cys Leu Asp Ala Thr Trp           
    130                 135                 140                           

tct ggt gca gca gtg tac att gat gat aat acg gct aaa gat ctt cat        480
Ser Gly Ala Ala Val Tyr Ile Asp Asp Asn Thr Ala Lys Asp Leu His           
145                 150                 155                 160           

aga cct tat gga agg gtt aac cgg aaa cag ctg aaa tcg aaa atg atg        528
Arg Pro Tyr Gly Arg Val Asn Arg Lys Gln Leu Lys Ser Lys Met Met           
                165                 170                 175               

cag aaa tgt ata atg aat ggt gtt aaa ttc cac caa gcc aaa gtt ata        576
Gln Lys Cys Ile Met Asn Gly Val Lys Phe His Gln Ala Lys Val Ile           
            180                 185                 190                   

aag gtg att cat gag gaa tcg aaa tcc atg ttg ata tgc aat gat ggt        624
Lys Val Ile His Glu Glu Ser Lys Ser Met Leu Ile Cys Asn Asp Gly           
        195                 200                 205                       

att act att cag gca acg gtg gtg ctc gat gca act ggc ttc tct aga        672
Ile Thr Ile Gln Ala Thr Val Val Leu Asp Ala Thr Gly Phe Ser Arg           
    210                 215                 220                           

tct ctt gtt cag tat gat aag cct tat aac ccc ggg tat caa gtt gct        720
Ser Leu Val Gln Tyr Asp Lys Pro Tyr Asn Pro Gly Tyr Gln Val Ala           
225                 230                 235                 240           

tat ggc att ttg gct gaa gtg gaa gag cac ccc ttt gat gta aac aag        768
Tyr Gly Ile Leu Ala Glu Val Glu Glu His Pro Phe Asp Val Asn Lys           
                245                 250                 255               

atg gtt ttc atg gat tgg cga gat tct cat ttg aag aac aat act gat        816
Met Val Phe Met Asp Trp Arg Asp Ser His Leu Lys Asn Asn Thr Asp           
            260                 265                 270                   

ctc aag gag aga aat agt aga ata cca act ttt ctt tat gca atg cca        864
Leu Lys Glu Arg Asn Ser Arg Ile Pro Thr Phe Leu Tyr Ala Met Pro           
        275                 280                 285                       

ttt tca tcc aac agg ata ttt ctt gaa gaa aca tca ctc gta gct cgt        912
Phe Ser Ser Asn Arg Ile Phe Leu Glu Glu Thr Ser Leu Val Ala Arg           
    290                 295                 300                           

cct ggc ttg cgt ata gat gat att caa gaa cga atg gtg gct cgt tta        960
Pro Gly Leu Arg Ile Asp Asp Ile Gln Glu Arg Met Val Ala Arg Leu           
305                 310                 315                 320           

aac cat ttg ggg ata aaa gtg aag agc att gaa gaa gat gaa cat tgt       1008
Asn His Leu Gly Ile Lys Val Lys Ser Ile Glu Glu Asp Glu His Cys           
                325                 330                 335               

cta ata cca atg ggt ggt cca ctt cca gta tta cct cag aga gtc gtt       1056
Leu Ile Pro Met Gly Gly Pro Leu Pro Val Leu Pro Gln Arg Val Val           
            340                 345                 350                   

gga atc ggt ggt aca gct ggc atg gtt cat cca tcc acc ggt tat atg       1104
Gly Ile Gly Gly Thr Ala Gly Met Val His Pro Ser Thr Gly Tyr Met           
        355                 360                 365                       

gtg gca agg aca cta gct gcg gct cct gtt gtt gcc aat gcc ata att       1152
Val Ala Arg Thr Leu Ala Ala Ala Pro Val Val Ala Asn Ala Ile Ile           
    370                 375                 380                           

caa tac ctc ggt tct gaa aga agt cat tcg ggt aat gaa tta tcc aca       1200
Gln Tyr Leu Gly Ser Glu Arg Ser His Ser Gly Asn Glu Leu Ser Thr           
385                 390                 395                 400           

gct gtt tgg aaa gat ttg tgg cct ata gag agg aga cgt caa aga gag       1248
Ala Val Trp Lys Asp Leu Trp Pro Ile Glu Arg Arg Arg Gln Arg Glu           
                405                 410                 415               

ttc ttc tgc ttc ggt atg gat att ctt ctg aag ctt gat tta cct gct       1296
Phe Phe Cys Phe Gly Met Asp Ile Leu Leu Lys Leu Asp Leu Pro Ala           
            420                 425                 430                   

aca aga agg ttc ttt gat gca ttc ttt gac tta gaa cct cgt tat tgg       1344
Thr Arg Arg Phe Phe Asp Ala Phe Phe Asp Leu Glu Pro Arg Tyr Trp           
        435                 440                 445                       

cat ggc ttc tta tcg tct cga ttg ttt cta cct gaa ctc ata gtt ttt       1392
His Gly Phe Leu Ser Ser Arg Leu Phe Leu Pro Glu Leu Ile Val Phe           
    450                 455                 460                           

ggg ctg tct cta ttc tct cat gct tca aat act tct aga ttt gag ata       1440
Gly Leu Ser Leu Phe Ser His Ala Ser Asn Thr Ser Arg Phe Glu Ile           
465                 470                 475                 480           

atg aca aag gga act gtt cca tta gta aat atg atc aac aat ttg tta       1488
Met Thr Lys Gly Thr Val Pro Leu Val Asn Met Ile Asn Asn Leu Leu           
                485                 490                 495               

cag gat aaa gaa tga                                                   1503
Gln Asp Lys Glu                                                           
            500                                                           


<210>  9
<211>  500
<212>  PRT
<213>  Solanum lycopersicum


<220>
<221>  MISC_FEATURE
<222>  (1)..(500)
<223>  Wild type aminoacid sequence of lycopene cyclase Lcy B

<400>  9

Met Asp Thr Leu Leu Lys Thr Pro Asn Asn Leu Glu Phe Leu Asn Pro 
1               5                   10                  15      


His His Gly Phe Ala Val Lys Ala Ser Thr Phe Arg Ser Glu Lys His 
            20                  25                  30          


His Asn Phe Gly Ser Arg Lys Phe Cys Glu Thr Leu Gly Arg Ser Val 
        35                  40                  45              


Cys Val Lys Gly Ser Ser Ser Ala Leu Leu Glu Leu Val Pro Glu Thr 
    50                  55                  60                  


Lys Lys Glu Asn Leu Asp Phe Glu Leu Pro Met Tyr Asp Pro Ser Lys 
65                  70                  75                  80  


Gly Val Val Val Asp Leu Ala Val Val Gly Gly Gly Pro Ala Gly Leu 
                85                  90                  95      


Ala Val Ala Gln Gln Val Ser Glu Ala Gly Leu Ser Val Cys Ser Ile 
            100                 105                 110         


Asp Pro Asn Pro Lys Leu Ile Trp Pro Asn Asn Tyr Gly Val Trp Val 
        115                 120                 125             


Asp Glu Phe Glu Ala Met Asp Leu Leu Asp Cys Leu Asp Ala Thr Trp 
    130                 135                 140                 


Ser Gly Ala Ala Val Tyr Ile Asp Asp Asn Thr Ala Lys Asp Leu His 
145                 150                 155                 160 


Arg Pro Tyr Gly Arg Val Asn Arg Lys Gln Leu Lys Ser Lys Met Met 
                165                 170                 175     


Gln Lys Cys Ile Met Asn Gly Val Lys Phe His Gln Ala Lys Val Ile 
            180                 185                 190         


Lys Val Ile His Glu Glu Ser Lys Ser Met Leu Ile Cys Asn Asp Gly 
        195                 200                 205             


Ile Thr Ile Gln Ala Thr Val Val Leu Asp Ala Thr Gly Phe Ser Arg 
    210                 215                 220                 


Ser Leu Val Gln Tyr Asp Lys Pro Tyr Asn Pro Gly Tyr Gln Val Ala 
225                 230                 235                 240 


Tyr Gly Ile Leu Ala Glu Val Glu Glu His Pro Phe Asp Val Asn Lys 
                245                 250                 255     


Met Val Phe Met Asp Trp Arg Asp Ser His Leu Lys Asn Asn Thr Asp 
            260                 265                 270         


Leu Lys Glu Arg Asn Ser Arg Ile Pro Thr Phe Leu Tyr Ala Met Pro 
        275                 280                 285             


Phe Ser Ser Asn Arg Ile Phe Leu Glu Glu Thr Ser Leu Val Ala Arg 
    290                 295                 300                 


Pro Gly Leu Arg Ile Asp Asp Ile Gln Glu Arg Met Val Ala Arg Leu 
305                 310                 315                 320 


Asn His Leu Gly Ile Lys Val Lys Ser Ile Glu Glu Asp Glu His Cys 
                325                 330                 335     


Leu Ile Pro Met Gly Gly Pro Leu Pro Val Leu Pro Gln Arg Val Val 
            340                 345                 350         


Gly Ile Gly Gly Thr Ala Gly Met Val His Pro Ser Thr Gly Tyr Met 
        355                 360                 365             


Val Ala Arg Thr Leu Ala Ala Ala Pro Val Val Ala Asn Ala Ile Ile 
    370                 375                 380                 


Gln Tyr Leu Gly Ser Glu Arg Ser His Ser Gly Asn Glu Leu Ser Thr 
385                 390                 395                 400 


Ala Val Trp Lys Asp Leu Trp Pro Ile Glu Arg Arg Arg Gln Arg Glu 
                405                 410                 415     


Phe Phe Cys Phe Gly Met Asp Ile Leu Leu Lys Leu Asp Leu Pro Ala 
            420                 425                 430         


Thr Arg Arg Phe Phe Asp Ala Phe Phe Asp Leu Glu Pro Arg Tyr Trp 
        435                 440                 445             


His Gly Phe Leu Ser Ser Arg Leu Phe Leu Pro Glu Leu Ile Val Phe 
    450                 455                 460                 


Gly Leu Ser Leu Phe Ser His Ala Ser Asn Thr Ser Arg Phe Glu Ile 
465                 470                 475                 480 


Met Thr Lys Gly Thr Val Pro Leu Val Asn Met Ile Asn Asn Leu Leu 
                485                 490                 495     


Gln Asp Lys Glu 
            500 


<210>  10
<211>  498
<212>  PRT
<213>  Capsicum annuum


<220>
<221>  MISC_FEATURE
<222>  (1)..(498)
<223>  Capsanthin/capsorubin synthase CCS

<400>  10

Met Glu Thr Leu Leu Lys Pro Phe Pro Ser Pro Leu Leu Ser Ile Pro 
1               5                   10                  15      


Thr Pro Asn Met Tyr Ser Phe Lys His Asn Ser Thr Phe Pro Asn Pro 
            20                  25                  30          


Thr Lys Gln Lys Asp Ser Arg Lys Phe His Tyr Arg Asn Lys Ser Ser 
        35                  40                  45              


Thr His Phe Cys Ser Phe Leu Asp Leu Ala Pro Thr Ser Lys Pro Glu 
    50                  55                  60                  


Ser Leu Asp Val Asn Ile Ser Trp Val Asp Thr Asp Leu Asp Gly Ala 
65                  70                  75                  80  


Glu Phe Asp Val Ile Ile Ile Gly Thr Gly Pro Ala Gly Leu Arg Leu 
                85                  90                  95      


Ala Glu Gln Val Ser Lys Tyr Gly Ile Lys Val Cys Cys Val Asp Pro 
            100                 105                 110         


Ser Pro Leu Ser Met Trp Pro Asn Asn Tyr Gly Val Trp Val Asp Glu 
        115                 120                 125             


Phe Glu Lys Leu Gly Leu Glu Asp Cys Leu Asp His Lys Trp Pro Val 
    130                 135                 140                 


Ser Cys Val His Ile Ser Asp His Lys Thr Lys Tyr Leu Asp Arg Pro 
145                 150                 155                 160 


Tyr Gly Arg Val Ser Arg Lys Lys Leu Lys Leu Lys Leu Leu Asn Ser 
                165                 170                 175     


Cys Val Glu Asn Arg Val Lys Phe Tyr Lys Ala Lys Val Leu Lys Val 
            180                 185                 190         


Lys His Glu Glu Phe Glu Ser Ser Ile Val Cys Asp Asp Gly Arg Lys 
        195                 200                 205             


Ile Ser Gly Ser Leu Ile Val Asp Ala Ser Gly Tyr Ala Ser Asp Phe 
    210                 215                 220                 


Ile Glu Tyr Asp Lys Pro Arg Asn His Gly Tyr Gln Val Ala His Gly 
225                 230                 235                 240 


Ile Leu Ala Glu Val Asp Asn His Pro Phe Asp Leu Asp Lys Met Met 
                245                 250                 255     


Leu Met Asp Trp Arg Asp Ser His Leu Gly Asn Glu Pro Tyr Leu Arg 
            260                 265                 270         


Val Lys Asn Thr Lys Glu Pro Thr Phe Leu Tyr Ala Met Pro Phe Asp 
        275                 280                 285             


Arg Asn Leu Val Phe Leu Glu Glu Thr Ser Leu Val Ser Arg Pro Met 
    290                 295                 300                 


Leu Ser Tyr Met Glu Val Lys Arg Arg Met Val Ala Arg Leu Arg His 
305                 310                 315                 320 


Leu Gly Ile Lys Val Arg Ser Val Leu Glu Glu Glu Lys Cys Val Ile 
                325                 330                 335     


Thr Met Gly Gly Pro Leu Pro Arg Ile Pro Gln Asn Val Met Ala Ile 
            340                 345                 350         


Gly Gly Thr Ser Gly Ile Val His Pro Ser Ser Gly Tyr Met Val Ala 
        355                 360                 365             


Arg Ser Met Ala Leu Ala Pro Val Leu Ala Glu Ala Ile Val Glu Ser 
    370                 375                 380                 


Leu Gly Ser Thr Arg Met Ile Arg Gly Ser Gln Leu Tyr His Arg Val 
385                 390                 395                 400 


Trp Asn Gly Leu Trp Pro Ser Asp Arg Arg Arg Val Arg Glu Cys Tyr 
                405                 410                 415     


Cys Phe Gly Met Glu Thr Leu Leu Lys Leu Asp Leu Glu Gly Thr Arg 
            420                 425                 430         


Arg Leu Phe Asp Ala Phe Phe Asp Val Asp Pro Lys Tyr Trp His Gly 
        435                 440                 445             


Phe Leu Ser Ser Arg Leu Ser Val Lys Glu Leu Ala Val Leu Ser Leu 
    450                 455                 460                 


Tyr Leu Phe Gly His Ala Ser Asn Leu Ala Arg Leu Asp Ile Val Thr 
465                 470                 475                 480 


Lys Cys Thr Val Pro Leu Val Lys Leu Leu Gly Asn Leu Ala Ile Glu 
                485                 490                 495     


Ser Leu 
        


