                         SEQUENCE LISTING

<110>  SYNTHETIC GENOMICS, INC.
       VERRUTO, John
       MOELLERING, Eric
 
<120>  COMPOSITIONS AND METHODS FOR HIGH EFFICIENCY IN VIVO GENOME 
       EDITING

<130>  SGI1850-1WO

<150>  US 62/099,014
<151>  2014-12-24

<160>  118   

<170>  PatentIn version 3.5

<210>  1
<211>  4101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. pyogenes


<220>
<221>  misc_feature
<223>  CAS9 gene codon optimized for Nannochloropsis

<400>  1
gacaagaagt actccatcgg gctggacatc gggacgaact ccgtgggatg ggccgtgatc       60

acagacgaat acaaggtgcc ttccaagaag ttcaaggtgc tggggaacac ggacagacac      120

tccatcaaga agaacctcat cggggccttg ctcttcgact ccggagaaac cgccgaagca      180

acgcgattga aaagaaccgc cagaagacga tacacacgac ggaagaaccg catctgctac      240

ctccaggaga tcttcagcaa cgagatggcc aaggtggacg actcgttctt tcatcgcctg      300

gaggagagct tcctggtgga ggaagacaag aaacatgagc gccacccgat cttcgggaac      360

atcgtggacg aagtggccta ccacgagaaa taccccacga tctaccactt gcgcaagaaa      420

ctcgtggact ccacggacaa agcggacttg cggttgatct acttggcctt ggcccacatg      480

atcaaatttc ggggccactt cctgatcgag ggcgacttga atcccgacaa ttccgacgtg      540

gacaagctct tcatccagct ggtgcagacc tacaaccagc tcttcgagga gaaccccatc      600

aatgcctccg gagtggacgc caaagccatc ttgtccgccc gattgtccaa atccagacgc      660

ttggagaact tgatcgcaca acttcctggc gagaagaaga acggcctctt cggcaacttg      720

atcgcgctgt cgctgggatt gacgcctaac ttcaagtcca acttcgactt ggccgaggac      780

gccaagttgc aactgtccaa ggacacctac gacgacgacc tcgacaacct gctggcccaa      840

attggcgacc aatacgcgga cttgtttttg gcggccaaga acttgagcga cgccatcttg      900

ttgagcgaca tcttgcgcgt gaatacggag atcaccaaag cccctttgtc cgcctctatg      960

atcaagcggt acgacgagca ccaccaagac ttgaccctgt tgaaagccct cgtgcggcaa     1020

caattgcccg agaagtacaa ggagatcttc ttcgaccagt ccaagaacgg gtacgccggc     1080

tacatcgacg gaggagcctc ccaagaagag ttctacaagt tcatcaagcc catcctggag     1140

aagatggacg gcaccgagga gttgctcgtg aagctgaacc gcgaagactt gttgcgaaaa     1200

cagcggacgt tcgacaatgg cagcatcccc caccaaatcc atttgggaga gttgcacgcc     1260

atcttgcgac ggcaagagga cttctacccg ttcctgaagg acaaccgcga gaaaatcgag     1320

aagatcctga cgttcagaat cccctactac gtgggaccct tggcccgagg caattcccgg     1380

tttgcatgga tgacgcgcaa aagcgaagag acgatcaccc cctggaactt cgaagaagtg     1440

gtcgacaaag gagcatccgc acagagcttc atcgagcgaa tgacgaactt cgacaagaac     1500

ctgcccaacg agaaggtgtt gcccaagcat tcgctgctgt acgagtactt cacggtgtac     1560

aacgagctga ccaaggtgaa gtacgtgacc gagggcatgc gcaaacccgc gttcctgtcg     1620

ggagagcaaa agaaggccat tgtggacctg ctgttcaaga ccaaccggaa ggtgaccgtg     1680

aaacagctga aagaggacta cttcaagaag atcgagtgct tcgactccgt ggagatctcc     1740

ggcgtggagg accgattcaa tgcctccttg ggaacctacc atgacctcct gaagatcatc     1800

aaggacaagg acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg     1860

accctgaccc tgttcgagga ccgagagatg atcgaggaac ggttgaaaac gtacgcccac     1920

ttgttcgacg acaaggtgat gaagcagctg aaacgccgcc gctacaccgg atggggacga     1980

ttgagccgca aactgattaa tggaattcgc gacaagcaat ccggaaagac catcctggac     2040

ttcctgaagt ccgacgggtt cgccaaccgc aacttcatgc agctcatcca cgacgactcc     2100

ttgaccttca aggaggacat ccagaaggcc caagtgtccg gacaaggaga ctccttgcac     2160

gagcacatcg ccaatttggc cggatccccc gcaatcaaaa aaggcatctt gcaaaccgtg     2220

aaagtggtcg acgaactggt gaaggtgatg ggacggcaca agcccgagaa catcgtgatc     2280

gaaatggccc gcgagaacca aaccacccaa aaaggacaga agaactcccg agagcgcatg     2340

aagcggatcg aagagggcat caaggagttg ggctcccaga tcctgaagga gcatcccgtg     2400

gagaataccc aattgcaaaa cgagaagctc tacctctact acctccagaa cgggcgggac     2460

atgtacgtcg accaagagct ggacatcaac cgcctctccg actacgatgt ggatcatatt     2520

gtgccccaga gcttcctcaa ggacgacagc atcgacaaca aggtcctgac gcgcagcgac     2580

aagaaccggg gcaagtctga caatgtgcct tccgaagaag tcgtgaagaa gatgaagaac     2640

tactggcggc agctgctcaa cgccaagctc atcacccaac ggaagttcga caacctgacc     2700

aaggccgaga gaggaggatt gtccgagttg gacaaagccg gcttcattaa acgccaactc     2760

gtggagaccc gccagatcac gaagcacgtg gcccaaatct tggactcccg gatgaacacg     2820

aaatacgacg agaatgacaa gctgatccgc gaggtgaagg tgatcacgct gaagtccaag     2880

ctggtgagcg acttccggaa ggacttccag ttctacaagg tgcgggagat caacaactac     2940

catcacgccc atgacgccta cctgaacgcc gtggtcggaa ccgccctgat caagaaatac     3000

cccaagctgg agtccgaatt cgtgtacgga gattacaagg tctacgacgt gcggaagatg     3060

atcgcgaagt ccgagcagga gatcggcaaa gccaccgcca agtacttctt ttactccaac     3120

atcatgaact tcttcaagac cgagatcacg ctcgccaacg gcgagatccg caagcgcccc     3180

ctgatcgaga ccaacggcga gacgggagag attgtgtggg acaaaggaag agattttgcc     3240

acagtgcgca aggtgctgtc catgcctcag gtgaacatcg tgaagaagac cgaggtgcaa     3300

acaggagggt tttccaaaga gtccattttg cctaagagga attccgacaa gctcatcgcc     3360

cgcaagaagg actgggaccc caagaagtac gggggcttcg actcccccac ggtggcctac     3420

tccgtgttgg tggtggccaa agtggagaaa gggaagagca agaagctgaa atccgtgaag     3480

gagttgctcg gaatcacgat catggaacga tcgtcgttcg agaaaaaccc catcgacttc     3540

ctcgaagcca aagggtacaa agaggtgaag aaggacctga tcatcaagct gcccaagtac     3600

tccctgttcg agctggagaa cggccgcaag cggatgctgg cctccgccgg ggaactgcag     3660

aaagggaacg aattggcctt gccctccaaa tacgtgaact tcctctactt ggcctcccat     3720

tacgaaaagc tcaaaggatc ccctgaggac aatgagcaga agcaactctt cgtggaacaa     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gcgcgtgatc     3840

ctcgccgacg ccaacctgga caaggtgctc tccgcctaca acaagcaccg cgacaagcct     3900

atccgcgagc aagccgagaa tatcattcac ctgtttaccc tgacgaattt gggagcccct     3960

gccgccttta aatactttga caccaccatc gaccgcaaaa gatacacctc caccaaggaa     4020

gtcttggacg ccaccctcat ccaccagtcc atcacgggcc tctacgagac gcgcatcgac     4080

ctctcccaat tgggcggcga c                                               4101


<210>  2
<211>  1367
<212>  PRT
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<223>  Cas9

<400>  2

Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          


Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              


Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys 
    50                  55                  60                  


Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  


Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      


Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         


Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             


Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 


Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 


Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     


Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         


Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             


Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 


Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 


Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     


Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         


Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             


Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 


Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 


Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     


Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         


Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             


Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 


Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 


Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     


Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         


Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 


Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 


Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     


Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         


Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             


Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 


Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 


Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     


Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 


Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 


Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 


Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1100                 1105                 1110             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1145                 1150                 1155             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1160                 1165                 1170             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1175                 1180                 1185             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1190                 1195                 1200             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1205                 1210                 1215             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1220                 1225                 1230             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1235                 1240                 1245             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1250                 1255                 1260             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1265                 1270                 1275             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1280                 1285                 1290             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1295                 1300                 1305             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1325                 1330                 1335             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  3
<211>  8
<212>  PRT
<213>  SV40 virus


<220>
<221>  misc_feature
<223>  nuclear localization signal peptide

<400>  3

Pro Lys Lys Lys Arg Lys Val Gly 
1               5               


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encoding codon optimized SV40 nuclear localization sequence

<400>  4
cccaagaaaa agcggaaggt cggc                                              24


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encoding FLAG tag

<400>  5
gactacaagg atgacgatga caag                                              24


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag peptide

<400>  6

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  7
<211>  4158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered Cas9 gene encoding NLS-Cas9-C terminal FLAG tag

<400>  7
atggctccca agaaaaagcg gaaggtcggc gacaagaagt actccatcgg gctggacatc       60

gggacgaact ccgtgggatg ggccgtgatc acagacgaat acaaggtgcc ttccaagaag      120

ttcaaggtgc tggggaacac ggacagacac tccatcaaga agaacctcat cggggccttg      180

ctcttcgact ccggagaaac cgccgaagca acgcgattga aaagaaccgc cagaagacga      240

tacacacgac ggaagaaccg catctgctac ctccaggaga tcttcagcaa cgagatggcc      300

aaggtggacg actcgttctt tcatcgcctg gaggagagct tcctggtgga ggaagacaag      360

aaacatgagc gccacccgat cttcgggaac atcgtggacg aagtggccta ccacgagaaa      420

taccccacga tctaccactt gcgcaagaaa ctcgtggact ccacggacaa agcggacttg      480

cggttgatct acttggcctt ggcccacatg atcaaatttc ggggccactt cctgatcgag      540

ggcgacttga atcccgacaa ttccgacgtg gacaagctct tcatccagct ggtgcagacc      600

tacaaccagc tcttcgagga gaaccccatc aatgcctccg gagtggacgc caaagccatc      660

ttgtccgccc gattgtccaa atccagacgc ttggagaact tgatcgcaca acttcctggc      720

gagaagaaga acggcctctt cggcaacttg atcgcgctgt cgctgggatt gacgcctaac      780

ttcaagtcca acttcgactt ggccgaggac gccaagttgc aactgtccaa ggacacctac      840

gacgacgacc tcgacaacct gctggcccaa attggcgacc aatacgcgga cttgtttttg      900

gcggccaaga acttgagcga cgccatcttg ttgagcgaca tcttgcgcgt gaatacggag      960

atcaccaaag cccctttgtc cgcctctatg atcaagcggt acgacgagca ccaccaagac     1020

ttgaccctgt tgaaagccct cgtgcggcaa caattgcccg agaagtacaa ggagatcttc     1080

ttcgaccagt ccaagaacgg gtacgccggc tacatcgacg gaggagcctc ccaagaagag     1140

ttctacaagt tcatcaagcc catcctggag aagatggacg gcaccgagga gttgctcgtg     1200

aagctgaacc gcgaagactt gttgcgaaaa cagcggacgt tcgacaatgg cagcatcccc     1260

caccaaatcc atttgggaga gttgcacgcc atcttgcgac ggcaagagga cttctacccg     1320

ttcctgaagg acaaccgcga gaaaatcgag aagatcctga cgttcagaat cccctactac     1380

gtgggaccct tggcccgagg caattcccgg tttgcatgga tgacgcgcaa aagcgaagag     1440

acgatcaccc cctggaactt cgaagaagtg gtcgacaaag gagcatccgc acagagcttc     1500

atcgagcgaa tgacgaactt cgacaagaac ctgcccaacg agaaggtgtt gcccaagcat     1560

tcgctgctgt acgagtactt cacggtgtac aacgagctga ccaaggtgaa gtacgtgacc     1620

gagggcatgc gcaaacccgc gttcctgtcg ggagagcaaa agaaggccat tgtggacctg     1680

ctgttcaaga ccaaccggaa ggtgaccgtg aaacagctga aagaggacta cttcaagaag     1740

atcgagtgct tcgactccgt ggagatctcc ggcgtggagg accgattcaa tgcctccttg     1800

ggaacctacc atgacctcct gaagatcatc aaggacaagg acttcctgga caacgaggag     1860

aacgaggaca tcctggagga catcgtgctg accctgaccc tgttcgagga ccgagagatg     1920

atcgaggaac ggttgaaaac gtacgcccac ttgttcgacg acaaggtgat gaagcagctg     1980

aaacgccgcc gctacaccgg atggggacga ttgagccgca aactgattaa tggaattcgc     2040

gacaagcaat ccggaaagac catcctggac ttcctgaagt ccgacgggtt cgccaaccgc     2100

aacttcatgc agctcatcca cgacgactcc ttgaccttca aggaggacat ccagaaggcc     2160

caagtgtccg gacaaggaga ctccttgcac gagcacatcg ccaatttggc cggatccccc     2220

gcaatcaaaa aaggcatctt gcaaaccgtg aaagtggtcg acgaactggt gaaggtgatg     2280

ggacggcaca agcccgagaa catcgtgatc gaaatggccc gcgagaacca aaccacccaa     2340

aaaggacaga agaactcccg agagcgcatg aagcggatcg aagagggcat caaggagttg     2400

ggctcccaga tcctgaagga gcatcccgtg gagaataccc aattgcaaaa cgagaagctc     2460

tacctctact acctccagaa cgggcgggac atgtacgtcg accaagagct ggacatcaac     2520

cgcctctccg actacgatgt ggatcatatt gtgccccaga gcttcctcaa ggacgacagc     2580

atcgacaaca aggtcctgac gcgcagcgac aagaaccggg gcaagtctga caatgtgcct     2640

tccgaagaag tcgtgaagaa gatgaagaac tactggcggc agctgctcaa cgccaagctc     2700

atcacccaac ggaagttcga caacctgacc aaggccgaga gaggaggatt gtccgagttg     2760

gacaaagccg gcttcattaa acgccaactc gtggagaccc gccagatcac gaagcacgtg     2820

gcccaaatct tggactcccg gatgaacacg aaatacgacg agaatgacaa gctgatccgc     2880

gaggtgaagg tgatcacgct gaagtccaag ctggtgagcg acttccggaa ggacttccag     2940

ttctacaagg tgcgggagat caacaactac catcacgccc atgacgccta cctgaacgcc     3000

gtggtcggaa ccgccctgat caagaaatac cccaagctgg agtccgaatt cgtgtacgga     3060

gattacaagg tctacgacgt gcggaagatg atcgcgaagt ccgagcagga gatcggcaaa     3120

gccaccgcca agtacttctt ttactccaac atcatgaact tcttcaagac cgagatcacg     3180

ctcgccaacg gcgagatccg caagcgcccc ctgatcgaga ccaacggcga gacgggagag     3240

attgtgtggg acaaaggaag agattttgcc acagtgcgca aggtgctgtc catgcctcag     3300

gtgaacatcg tgaagaagac cgaggtgcaa acaggagggt tttccaaaga gtccattttg     3360

cctaagagga attccgacaa gctcatcgcc cgcaagaagg actgggaccc caagaagtac     3420

gggggcttcg actcccccac ggtggcctac tccgtgttgg tggtggccaa agtggagaaa     3480

gggaagagca agaagctgaa atccgtgaag gagttgctcg gaatcacgat catggaacga     3540

tcgtcgttcg agaaaaaccc catcgacttc ctcgaagcca aagggtacaa agaggtgaag     3600

aaggacctga tcatcaagct gcccaagtac tccctgttcg agctggagaa cggccgcaag     3660

cggatgctgg cctccgccgg ggaactgcag aaagggaacg aattggcctt gccctccaaa     3720

tacgtgaact tcctctactt ggcctcccat tacgaaaagc tcaaaggatc ccctgaggac     3780

aatgagcaga agcaactctt cgtggaacaa cacaagcact acctggacga gatcatcgag     3840

cagatcagcg agttctccaa gcgcgtgatc ctcgccgacg ccaacctgga caaggtgctc     3900

tccgcctaca acaagcaccg cgacaagcct atccgcgagc aagccgagaa tatcattcac     3960

ctgtttaccc tgacgaattt gggagcccct gccgccttta aatactttga caccaccatc     4020

gaccgcaaaa gatacacctc caccaaggaa gtcttggacg ccaccctcat ccaccagtcc     4080

atcacgggcc tctacgagac gcgcatcgac ctctcccaat tgggcggcga cgactacaag     4140

gatgacgatg acaagtga                                                   4158


<210>  8
<211>  1385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS-Cas9-C terminal FLAG tag

<400>  8

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Asp Lys Lys Tyr Ser Ile 
1               5                   10                  15      


Gly Leu Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp 
            20                  25                  30          


Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp 
        35                  40                  45              


Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser 
    50                  55                  60                  


Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg 
65                  70                  75                  80  


Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser 
                85                  90                  95      


Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu 
            100                 105                 110         


Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe 
        115                 120                 125             


Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile 
    130                 135                 140                 


Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu 
145                 150                 155                 160 


Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His 
                165                 170                 175     


Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys 
            180                 185                 190         


Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn 
        195                 200                 205             


Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg 
    210                 215                 220                 


Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly 
225                 230                 235                 240 


Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly 
                245                 250                 255     


Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys 
            260                 265                 270         


Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu 
        275                 280                 285             


Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn 
    290                 295                 300                 


Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu 
305                 310                 315                 320 


Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu 
                325                 330                 335     


His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu 
            340                 345                 350         


Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr 
        355                 360                 365             


Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe 
    370                 375                 380                 


Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val 
385                 390                 395                 400 


Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn 
                405                 410                 415     


Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu 
            420                 425                 430         


Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys 
        435                 440                 445             


Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu 
    450                 455                 460                 


Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu 
465                 470                 475                 480 


Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser 
                485                 490                 495     


Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro 
            500                 505                 510         


Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr 
        515                 520                 525             


Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg 
    530                 535                 540                 


Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu 
545                 550                 555                 560 


Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp 
                565                 570                 575     


Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val 
            580                 585                 590         


Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys 
        595                 600                 605             


Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile 
    610                 615                 620                 


Leu Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met 
625                 630                 635                 640 


Ile Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val 
                645                 650                 655     


Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser 
            660                 665                 670         


Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile 
        675                 680                 685             


Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln 
    690                 695                 700                 


Leu Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala 
705                 710                 715                 720 


Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu 
                725                 730                 735     


Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val 
            740                 745                 750         


Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile 
        755                 760                 765             


Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys 
    770                 775                 780                 


Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu 
785                 790                 795                 800 


Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln 
                805                 810                 815     


Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr 
            820                 825                 830         


Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp 
        835                 840                 845             


His Ile Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys 
    850                 855                 860                 


Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro 
865                 870                 875                 880 


Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu 
                885                 890                 895     


Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala 
            900                 905                 910         


Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg 
        915                 920                 925             


Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu 
    930                 935                 940                 


Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg 
945                 950                 955                 960 


Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg 
                965                 970                 975     


Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His 
            980                 985                 990         


Ala His Asp Ala Tyr Leu Asn Ala  Val Val Gly Thr Ala  Leu Ile Lys 
        995                 1000                 1005             


Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val Tyr Gly  Asp Tyr Lys 
    1010                 1015                 1020             


Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser Glu  Gln Glu Ile 
    1025                 1030                 1035             


Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  Ile Met Asn 
    1040                 1045                 1050             


Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn Gly Glu  Ile Arg Lys 
    1055                 1060                 1065             


Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr Gly Glu  Ile Val Trp 
    1070                 1075                 1080             


Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg Lys Val  Leu Ser Met 
    1085                 1090                 1095             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly 
    1100                 1105                 1110             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg Asn Ser  Asp Lys Leu 
    1115                 1120                 1125             


Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys Lys Tyr  Gly Gly Phe 
    1130                 1135                 1140             


Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu Val Val  Ala Lys Val 
    1145                 1150                 1155             


Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser Val Lys  Glu Leu Leu 
    1160                 1165                 1170             


Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe Glu Lys  Asn Pro Ile 
    1175                 1180                 1185             


Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu Val Lys  Lys Asp Leu 
    1190                 1195                 1200             


Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly 
    1205                 1210                 1215             


Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu Leu Gln  Lys Gly Asn 
    1220                 1225                 1230             


Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn Phe Leu  Tyr Leu Ala 
    1235                 1240                 1245             


Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro Glu Asp  Asn Glu Gln 
    1250                 1255                 1260             


Lys Gln  Leu Phe Val Glu Gln  His Lys His Tyr Leu  Asp Glu Ile 
    1265                 1270                 1275             


Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg Val Ile  Leu Ala Asp 
    1280                 1285                 1290             


Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr Asn Lys  His Arg Asp 
    1295                 1300                 1305             


Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile Ile His  Leu Phe Thr 
    1310                 1315                 1320             


Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe Lys Tyr  Phe Asp Thr 
    1325                 1330                 1335             


Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr Lys Glu  Val Leu Asp 
    1340                 1345                 1350             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1355                 1360                 1365             


Ile Asp  Leu Ser Gln Leu Gly  Gly Asp Asp Tyr Lys  Asp Asp Asp 
    1370                 1375                 1380             


Asp Lys  
    1385 


<210>  9
<211>  1000
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  RPL7 promoter

<400>  9
gaatgctggc gacctcatga actttgttga tttttttaga attgtgtcat cgaaaaatat       60

acaatgtcga agtaaagttt atacactctg ggtactcttc ttctttgcca tccagtaggt      120

gtgggatagg agtgcgtggt aattctctga gacagttttc ctaccatgtt tcgggtccac      180

atagtacaag ttgttactcg tgacacctca tttcgctttt gtttctccac taatggacta      240

catttttcaa agcagcagcg tccgcgtttc tcgattaaga atctcccgtc cgctttgagt      300

ttgtttattt taaggattag aaggataaaa gcggagctgc aaaggtgaaa catgcctgag      360

aggactagaa gacatacgga gctgaagtgc ggggaggtta aacccgatga tcccattcct      420

tgcatttgtc aaggctcacc gcattcatag ttctaaaaag aaaatatttc cgaataatgt      480

taacacaaaa ataattaaat actgttctat gtactccgtt catttgttga tggaatcaaa      540

aggccagaaa aaaagagggg gggactccaa gctgccgcag acgctcgcct cgtctctcgt      600

tcgcggaatg ccggtcgtgg aaccgctgtg aagataccgt gccttgggga tcaattcaca      660

cattgacgag ggcgcctcac gccgtggcgt acttattcgg gccaaaatgt tatttccaag      720

gggtgacgga tggcatgctc gactcgtgtg atggctgatg catcaaccct ttcaatgctc      780

cttcctactt cctcttatcc actggtagtc tgtcatgaga tgtggtgaac cgcgtccgtg      840

ataatagtgt tctcatttaa cctaacaacc aacacaatac aaaacttgat tagataccca      900

gtgcatcgaa ttttgggtgc cttcaccgac aagcttgcac tttctcgagg gacgacaaca      960

acaaaccacc tacagcatta ccgggcaata gcagcacaac                           1000


<210>  10
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aspergillus terreus


<220>
<221>  misc_feature
<223>  BLAST gene codon optimized for N. gaditana

<400>  10
atggccaagc ctttatccca agaggaatcc acgctgatcg aacgtgcaac tgcgaccatc       60

aacagcatac ctattagcga ggactactcg gtggccagtg cagccctctc gtccgacggt      120

cggatcttta ccggcgtgaa tgtatatcat ttcaccggag ggccatgcgc ggagctcgtg      180

gtcctcggaa cggccgctgc ggctgctgcc ggaaatctga cgtgcatagt ggccatcggg      240

aacgaaaacc gcggcattct gtctccgtgc gggcgatgtc ggcaggtgct gcttgacttg      300

cacccgggga tcaaggcaat tgtcaaagat tccgatgggc agcccacagc ggttggcatc      360

agggagttgc ttccctctgg ctacgtctgg gagggttga                             399


<210>  11
<211>  999
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  TCTP promoter

<400>  11
cgtgcaggtg tacagattga aggaaacaat ggagatatct ttggcagttg aaaaccgtgt       60

tcgaatcatg cttttctact ctccaactga gacgaaattt atagcgccat gtcgcttctg      120

actaccaggc ttaggaaggc ctcatcacaa gctggatcgg ttcgaattaa gcaggcactg      180

aagccaagct tgcaagacag ccacctttta attccctcaa aacactttct caattcagcc      240

cggtaaatat gccgattcac agcggccaag atagagggga ggttagcaag aatgttgcga      300

tccctcccca gtcgttgcct cgcacacaac ctaggccttc acctttccat ggaaaattga      360

gaagtgaata ttggttttct tacggcatat cagatgaaat catgacccct aaacatgaag      420

agctgcaggc aaaacacctg ctctggacga gcacgatgaa atctcgagaa cccgccgtac      480

ttcagttgat cccgcatgat gacggccgcc attgaaataa gccacctcac tttattctag      540

caccgatttc caccgttgtg agggccgaac gaggacaatt tcgtgcgaaa caagcacgaa      600

cacgcacacg attagtagta cagacgagca gatcgatggc atgcggcacg gtctcgcgtt      660

ctcggcgacc aggacaacgg agcagaggga ggcctgccga gttccgaggg gcattttagt      720

ccaaaattgt gttgacacgt gaacaagtgg cttgaaaaga ggaaggaaat gcctgggttt      780

cccttcgaga gcgggaactc gcttgtgcgt catcctagct acccatggtc cctttgtggg      840

ggaggctgtt tcgtcctacc gaatgtgtgg cgctccatgc atcttctgcc tcccaaacca      900

ccaacatgag cacgcgaagg aaggagaaaa aagtggccgc aacgttctct tctcatattt      960

attgtctcat cacaaacata ggtacataat acaacaatc                             999


<210>  12
<211>  318
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  EIF3 terminator

<400>  12
ggcactgtaa ccccggttcc gctcgacgaa ggctgggagc gccctttcgg tgggataaaa       60

tggatgcttt accgctgcgc ttcggctgag gaagagagaa atgcgagcgg ggatcggggt      120

cctagaaacg aagaaaggag aacaagttcc tggccaaaga aaaacaagac aaataccctc      180

tccaggcctg ggcccattac ttttttttgc tgtttcttat acctgcactc gtgcttctct      240

agtctgtcga gaccttacct gatcttcctc cctccatcgc tccccgcccc ccccatccga      300

gcaaccgtcg accatacg                                                    318


<210>  13
<211>  112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chimeric guide RNA targeting acyl-CoA oxidase gene

<400>  13
gacgggggcu guggcgcgcg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuuuuuccuu uu              112


<210>  14
<211>  20
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  20 nucleotide target sequence of acyl-CoA oxidase gene

<400>  14
gacgggggct gtggcgcgcg                                                   20


<210>  15
<211>  861
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  U6 promoter

<400>  15
gcaaaatcct tttcgatgct gccatttact tccacagtct tcaggtttaa ttgcttctcg       60

atgtcagcag tcatcaggag taccatcaaa ctttgaatta tcaagacccg cgcgaccaag      120

aatgccggga acgaatgaat taatatgcat aatacttata tatgtatgga tttgaagatg      180

caattcaaaa gctgggtctg agtacgtctc cgaaaaggac tcccaaaata ggacaagcca      240

tacgtacgcg tcttgcacgt aaaaggagac cgtcgaaaac gccactttta cgcattccag      300

gaaggaatag tctcttgcat ggcgacatca aaagtaacca cgtcaaattt caataacgtg      360

ttacatgttg ttggtcaatg ggtgcatgag tgtccgtatg tcacaagagg gtggggggct      420

cgagctggaa catctgctta ctgcacgcta ggaaggtcgc cataagtact ttgtgcaaat      480

tgtggaacca tcactccttc actacactac tcattatcaa tgaaatgttg acagctgttc      540

ctagtcgtgg ttgtgtcaga ttttccctgc atttgcccaa ctaaaggcac taaagccgac      600

tggcatgcca gtaggattat ggtagttgcc tcgcattata tctctctcgt ccgtcttgat      660

agccagaaaa tacgtaacca ctacccatac ggcaagcatg tccatgtata aaggttactc      720

tgcaaagaaa caatttgaaa atcccagaaa agagagtgtt tgaaaaaacc ctgcccgata      780

tccacatgtt ccaaggccat tgcaaacaac accaagcttt atagctagca tggtatccgt      840

atttctcgtt tacgggaaac c                                                861


<210>  16
<211>  15
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  U6 terminator

<400>  16
tttttttttc ctttt                                                        15


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACO2-upstream Forward Primer

<400>  17
tcaaagatca tttagcagag acgggggctg tggcgcg                                37


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACO2-downstream Reverse Primer

<400>  18
agtcgagaga tggtgcgttc acggatctag ccagag                                 36


<210>  19
<211>  853
<212>  DNA
<213>  Nannochloropsis gaditana

<400>  19
tcaaagatca tttagcagag acgggggctg tggcgcgcgc ggtacgtgtg gagtcttgct       60

ttcttcaggg ggggtcccgg ggggggggcg agtcagacaa agggtagagc gtactggaag      120

gacagacaga tgctggcatg accctgcatc cagaacacac atttaataat tgagggtctg      180

gtgatcgagg ggctcggcaa ccgcgcggct gctagcacgt gacacggttt tttacctcta      240

ctgttccaca atcaacccct acttcccctt ctccctccct ctttccctcc ctcccctacg      300

ttaattttcc ctcccgctgt actatatcgc ttcactttca gccgattagc tcctctgcca      360

tcaatgccac gccttttgcg gcgaggacga cgcataccat ggagcgcatg gcaagggaac      420

gagccaaggc ctccttcccc gtccgagaca tgacgtactt cttggacggc gggaggagca      480

tgaccgaggt caaggtgggt gaaataaaaa cataaaaaga gaataaacaa cgaaaggcga      540

ggtctttggg ggatgcctga atacgtaggc aaccgtactg atcgttttgg acccttcttc      600

tcttggggcc gatggcccac aggagggcat gatggcggac ttggcggcga atccggtctt      660

tacggaccca gaatggaacg acttgaacag agatcaggta gggatatggg tgattggccg      720

gaaggtgggg aaggaggggg aggtgcccct tgtgagcttg ccctgtggtc ggagcgcttc      780

cctcgatcaa cccctccctc tcccttcccc ttcccccctc tggctagatc cgtgaacgca      840

ccatctctcg act                                                         853


<210>  20
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chimeric guide RNA for knocking out CHORD gene

<400>  20
ggaggcuggu caggaugaaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu                        103


<210>  21
<211>  335
<212>  PRT
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  CHORD protein

<400>  21

Met Lys Leu Tyr Val His Tyr Glu Glu Ala Gly Gln Asp Glu Lys Ala 
1               5                   10                  15      


Leu Thr Leu Lys Leu Thr Leu Pro Lys Ser Trp Ala Glu Gln Pro Leu 
            20                  25                  30          


Leu Gln Val Leu Glu Leu Phe Ile Glu Ser Tyr Asn Lys Lys Lys Thr 
        35                  40                  45              


Gly Leu Pro Pro Leu Asp Lys Asp Phe Val His Met Glu Lys Ala Gly 
    50                  55                  60                  


Gly Val Ile Leu Pro Val Gly Asn Ile Val Ser Asp Met Leu Ser Asp 
65                  70                  75                  80  


Arg Asp Asp Leu Tyr Ile Arg Ser Gly Pro Gly Pro Ala Arg Gly Lys 
                85                  90                  95      


Ile Ala His Leu Ser Ser Pro Pro Asn Ala His Ala Ser Ser Glu Ser 
            100                 105                 110         


Ser Thr Gly Leu Leu Arg Cys Lys Asn Tyr Gly Cys Asn Gln Ser Phe 
        115                 120                 125             


Ser Glu Glu Asn Asn Ser Glu Glu Ala Cys Arg Phe His Lys Ala Pro 
    130                 135                 140                 


Pro Val Phe His Asp Thr Lys Lys Gly Trp Ser Cys Cys Ala Lys Arg 
145                 150                 155                 160 


Val Tyr Asp Trp Asp Glu Phe His Thr Ile Glu Gly Cys Thr Thr Gly 
                165                 170                 175     


Arg His Ser Leu Ile Asp Pro Lys Glu Ile Phe Ala Pro Ser Pro Thr 
            180                 185                 190         


Leu Ala Ala Ala Ala Gln Ala Glu Arg Gly Asp Cys Ser Asn Thr Ser 
        195                 200                 205             


Ser Ala Ala Thr Val Ile Lys Ser Ile Asp Glu Phe Asn Gln Ser Asn 
    210                 215                 220                 


Pro Asn Ala Ala Ala Ala Cys Lys Thr Ala Ala Ser Met Thr Leu Ala 
225                 230                 235                 240 


Gly Thr Arg Cys Thr Val Lys Pro Asp Gly Ser Ala Thr Cys Leu Asn 
                245                 250                 255     


Lys Gly Cys Gln Lys Asp Tyr Leu Leu Lys Glu Asn His Pro Ser Ala 
            260                 265                 270         


Cys Arg Tyr His Ala Ala Gly Pro Val Phe His Asp Ala Gly Lys Tyr 
        275                 280                 285             


Trp Ser Cys Cys Pro Gly Thr Val Lys Tyr Asp Phe Asp Asp Phe Leu 
    290                 295                 300                 


Lys Ile Pro Gly Cys Met Leu Ser Ser His Tyr Asp Gly Ser Gln Glu 
305                 310                 315                 320 


Ser Leu Glu Ala Phe Thr Arg His Ala Lys Thr Ser Glu Gly Thr 
                325                 330                 335 


<210>  22
<211>  1029
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hygromycin resistance gene, codon optimized for Nannochloropsis

<400>  22
atggggaaga aaccggaact gaccgctacg tccgtggaga aattccttat tgagaagttc       60

gactctgtct ccgacttgat gcaactgagc gagggagagg agagtagggc gttctcgttt      120

gacgtagggg gtcggggata cgtgttgagg gttaatagtt gtgcggacgg gttctacaag      180

gatcggtatg tctaccgtca tttcgcctcc gccgctctcc ccataccaga ggtactggac      240

attggggagt ttagcgaatc tctcacgtac tgcatctcgc gccgagccca gggagtgacg      300

ttgcaagatc tgcccgaaac tgaattgcct gccgttttgc aacccgtggc cgaggccatg      360

gacgcgatcg ctgccgcaga tctgtctcag acgtccggct ttggaccttt tgggccccag      420

ggcatcgggc agtacacgac ctggcgagac ttcatctgcg ccattgccga tcctcacgtc      480

tatcattggc agacagtcat ggatgacacc gtgtctgcat ccgtggccca agcactggac      540

gaactcatgt tgtgggccga ggattgccct gaggtcaggc acctggtgca cgcggatttc      600

ggcagcaata acgtacttac agacaatggt cggattactg ctgtcatcga ctggtccgaa      660

gcgatgtttg gtgatagcca atacgaagtg gcgaacatat tcttctggcg tccctggttg      720

gcgtgcatgg agcagcagac acgctacttt gaacggaggc acccggagct ggccggctcc      780

ccacgactcc gcgcctatat gttgcgtatc ggactcgatc agctttacca gtctctcgtc      840

gacggcaact tcgacgacgc cgcgtgggcg cagggccgct gcgacgcgat agtccgcagc      900

ggggctggga cggtgggtcg gacccaaatc gcacgccggt cggctgcggt gtggacagac      960

ggctgtgttg aggtgcttgc ggactcgggc aaccgtaggc cgagcacccg accgcgtgca     1020

aaggagtga                                                             1029


<210>  23
<211>  1000
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  EIF3 promoter

<400>  23
tcataatcaa agatgagcca gccacgaagc taccggagaa ttctgtaaga aaaatgttta       60

aagttgaaaa tgctaacagt gaagtgatat ccttttttaa tggagtgttg aggtgaagtc      120

tagcatcgta ggggaaaaca ggattctgtg tcttccattc tactccttga taaagcgaag      180

aaatccgaca aaaccaaaga gattgttcaa gtttaagatt tgtaagcgta caactatgaa      240

cttcttctct ttgtaggcct gagtggtcgt atgcatacga ttcatgaagt gaatcagtat      300

cgctggattt tgcttaggag taaagcacaa ctaagaaaat atgctgcctg gcaggcatcc      360

tgagacatga ggcaagcgac gtagcaattg aatcctaatt taagccaggg catctgtatg      420

actctgttag ttaattgatg aaccaatgag ctttaaaaaa aaatcgttgc gcgtaatgta      480

gttttaattc tccgccttga ggtgcggggc catttcggac aaggttcttt ggacggagat      540

ggcagcatgt gtcccttctc caaattggtc cgtgtggtag ttgagatgct gccttaaaat      600

tctgctcggt catcctgcct tcgcattcac tcctttcgag ctgtcgggtt cctcacgagg      660

cctccgggag cggattgcgc agaaaggcga cccggagaca cagagaccat acaccgacta      720

aattgcactg gacgatacgg catggcgacg acgatggcca agcattgcta cgtgattatt      780

cgccttgtca ttcagggaga aatgatgaca tgtgtgggac ggtctttaca tgggaagagg      840

gcatgaaaat aacatggcct ggcgggatgg agcgtcacac ctgtgtatgc gttcgatcca      900

caagcaactc accatttgcg tcggggcctg tctccaatct gctttaggct acttttctct      960

aatttagcct attctataca gacagagaca cacagggatc                           1000


<210>  24
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TurboGFP gene codon optimized for N. gaditana

<400>  24
atgttggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc       60

accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc cgagcagggc      120

cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg      180

agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac      240

cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag      300

gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc      360

gacttcaagg tgatgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc      420

atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacga tctggatggc      480

agcttcaccc gcaccttcag cctgcgcgac ggcggctact acagctccgt ggtggacagc      540

cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc      600

gccttccgcc gcgtggagga ggatcacagc aacaccgagc tgggcatcgt ggagtaccag      660

cacgccttca agaccccgga tgcagatgcc ggtgaagaat aa                         702


<210>  25
<211>  1000
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  RPL24 promoter

<400>  25
aataagcata catcatatga atacaattca gcttaaattt atcatacaaa gatgtaagtg       60

cagcgtgggt ctgtaacgat cgggcgtaat ttaagataat gcgagggacc gggggaggtt      120

ttggaacgga atgaggaatg ggtcatggcc cataataata atatgggttt ggtcgcctcg      180

cacagcaacc gtacgtgcga aaaaggaaca gatccattta ataagttgaa cgttattctt      240

tcctatgcaa tgcgtgtatc ggaggcgaga gcaagtcata ggtggctgcg cacaataatt      300

gagtctcagc tgagcgccgt ccgcgggtgg tgtgagtggt catcctcctc ccggcctatc      360

gctcacatcg cctctcaatg gtggtggtgg ggcctgatat gacctcaatg ccgacccata      420

ttaaaaccca gtaaagcatt caccaacgaa cgaggggctc ttttgtgtgt gttttgagta      480

tgattttaca cctctttgtg catctctctg gtcttccttg gttcccgtag tttgggcatc      540

atcactcacg cttccctcga ccttcgttct tcctttacaa ccccgacaca ggtcagagtt      600

ggagtaatca aaaaaggggt gcacgaatga gatacattag attttgacag atatcctttt      660

actggagagg gttcaaggga tcaaatgaac agcgggcgtt ggcaatctag ggagggatcg      720

gaggttggca gcgagcgaaa gcgtgtccat ccttttggct gtcacacctc acgaaccaac      780

tgttagcagg ccagcacaga tgacatacga gaatctttat tatatcgtag accttatgtg      840

gatgaccttt ggtgctgtgt gtctggcaat gaacctgaag gcttgatagg gaggtggctc      900

ccgtaaaccc tttgtccttt ccacgctgag tctcccccgc actgtccttt atacaaattg      960

ttacagtcat ctgcaggcgg tttttctttg gcaggcaaag                           1000


<210>  26
<211>  317
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  Bidirectional terminator 2

<400>  26
agtgatgcgg cctttaggaa acaccacaaa agtaattgac aatctcagga acgatctgcg       60

tgtttacagc ttcccaaata acaattatac cacgtaccaa aaggggttta atgtatctca      120

caaattcttc taataggtac agcttctcaa attgggtgta tgatgtgaca cttcgtctca      180

cacacgtcac gataattcag cgtatggctt cccttcatca cattcacgca aacttctaca      240

caaccctggg catatttctt gtgttggcaa cactcccgaa atcgattctg cacacaatgg      300

ttcattcaat gattcaa                                                     317


<210>  27
<211>  10103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chord3-KOvector

<400>  27
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt       60

aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag      120

ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct      180

ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt      240

tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg      300

cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct      360

gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc      420

gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg      480

tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa      540

ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg      600

gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg      660

ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga      720

tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt      780

ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct      840

gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga      900

acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agtttaaaca gggggcatca      960

gaatctatgc cgtatatgag cttatgaatg tcatccatta tctcatgtct gtcaacgcgg     1020

tcctcgggtg taacaaaaca caggaggagc gtctcacttt tgaccagcag ctttctgcct     1080

tagagcgcac cgtccgtgtc aaagaaaatg agctcaaaga attggagctt ctatctagta     1140

cgtgacatta tataaggagg caaagtgctc tattaatatg ttgacgagaa ggtttaatac     1200

atgcagcagg taacagatgc aggacaagtg tgtgaaattt aaccggctac ttcctatgaa     1260

tcaaacagaa gatgcttaca atgcacagga gatcgctgaa caagaactag caaaagagcg     1320

ctctgaatat aatgaggagc gtgcgcgaag ggaaagaagg ctacgggaac gacagcgtaa     1380

tgcaaaatat tgtttacaaa gacaaatgca agagcaggag aagtcgcaag gcgaaagtgc     1440

gggtggtagc atatgccaac aggaaactga ccagcaggac gagcaagacg acgaagctgg     1500

tagcgaagat tacgtgacta cacagcaagt ggaagcggac agattaagaa ctttgacaat     1560

gcttgtcaaa agtataactg ggatcggtga tgcacaggca ttgcgctcaa aacttgagca     1620

ccaaggggag gtcttgcgga agtcacaaga aattagcaaa gataatcagg tgactacaag     1680

aatgatagac tggtactacc ccagactcgt tgacatccgg cgtgtcgagc gttcacatca     1740

gagatatgaa ggtagatgac ggtaaaagtt tcctaaaatc gagcacgcga cttttttttc     1800

ttcttcaata tctgagcagg atcgcttaga gaagctacag aaagagcggg acatcctctg     1860

gaatcaatta cagagttcca gatattcacc gcgccccctc aaggtctgta acgaataata     1920

agagaggtca tcacttggcc ctgatcgcga tgctgtagag gttattgatg aaaggggagg     1980

aaagaatggc aggggccgta tagaaataat cctcaggatt ttgaaggcgt gcgtcatcct     2040

agaccaaatt tgcaggaaag atgcttagaa atctcaaaat tgacgcggat atttttgact     2100

aatcgacaaa catgctttca cgaccacagt ccgaaggcct acttcgccag ctgttgcacg     2160

acaaggaaaa agtcaaggag atcgaaaata ggtgcgagta cttagcacaa cttgtggcac     2220

atttgcagtg tggtttagtg gagcgtttat ggcctttagt aatgcgaagc aatcggaatc     2280

accttgctgc ttcgccaaag acggaaaatc tcgatcgagt ggcgcttcaa cgtatgctta     2340

aaaagttaga agtccgctgc ttgcagctgc ttgacaccaa atccatccca caaaagcatt     2400

gaaattctca aatatgggga accaaacgct catccaaccc atcccaagcc ccaggcttgc     2460

ttctgtcgaa tccgcagcac tcagcacgca tgtgtagtat cttttggata aatcattgca     2520

aagaaggtgt ataaggcttt ttcccggaag caaaagctcc cgtgggcact gtagaaacaa     2580

aagtgaggtg atagttattt ttcgatgcac ggaacggtat atatattcgc aaagcttgtg     2640

gaggctatcg ctgtcctttg tttgaagcag tcttcagcga agctgccacg gagcttcgtt     2700

caggaccgtt tcttggcatt caaaactcta cgactatgat actatcgagc attccgcatg     2760

attacagcgc aaagcctacc aaaaagatgc ggtaattcac ttgcgggacc gcaatcattg     2820

tcagccatca gcgcactgta ctcgtaaaaa ggcgaacatt gtccggtaac tcgctcatgt     2880

ctcacttgag ctgatgtcct ctctaaaata atagggtcgg tcccaaactc ttttaaactc     2940

cgatgaaact ctatgtgcac tacgagcggc cgcaataagc atacatcata tgaatacaat     3000

tcagcttaaa tttatcatac aaagatgtaa gtgcagcgtg ggtctgtaac gatcgggcgt     3060

aatttaagat aatgcgaggg accgggggag gttttggaac ggaatgagga atgggtcatg     3120

gcccataata ataatatggg tttggtcgcc tcgcacagca accgtacgtg cgaaaaagga     3180

acagatccat ttaataagtt gaacgttatt ctttcctatg caatgcgtgt atcggaggcg     3240

agagcaagtc ataggtggct gcgcacaata attgagtctc agctgagcgc cgtccgcggg     3300

tggtgtgagt ggtcatcctc ctcccggcct atcgctcaca tcgcctctca atggtggtgg     3360

tggggcctga tatgacctca atgccgaccc atattaaaac ccagtaaagc attcaccaac     3420

gaacgagggg ctcttttgtg tgtgttttga gtatgatttt acacctcttt gtgcatctct     3480

ctggtcttcc ttggttcccg tagtttgggc atcatcactc acgcttccct cgaccttcgt     3540

tcttccttta caaccccgac acaggtcaga gttggagtaa tcaaaaaagg ggtgcacgaa     3600

tgagatacat tagattttga cagatatcct tttactggag agggttcaag ggatcaaatg     3660

aacagcgggc gttggcaatc tagggaggga tcggaggttg gcagcgagcg aaagcgtgtc     3720

catccttttg gctgtcacac ctcacgaacc aactgttagc aggccagcac agatgacata     3780

cgagaatctt tattatatcg tagaccttat gtggatgacc tttggtgctg tgtgtctggc     3840

aatgaacctg aaggcttgat agggaggtgg ctcccgtaaa ccctttgtcc tttccacgct     3900

gagtctcccc cgcactgtcc tttatacaaa ttgttacagt catctgcagg cggtttttct     3960

ttggcaggca aacatgttgg agagcgacga gagcggcctg cccgccatgg agatcgagtg     4020

ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac     4080

ccccgagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag     4140

cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag     4200

cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat     4260

cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg     4320

ccgcgtgatc ggcgacttca aggtgatggg caccggcttc cccgaggaca gcgtgatctt     4380

caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa     4440

cgatctggat ggcagcttca cccgcacctt cagcctgcgc gacggcggct actacagctc     4500

cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg     4560

gggccccatg ttcgccttcc gccgcgtgga ggaggatcac agcaacaccg agctgggcat     4620

cgtggagtac cagcacgcct tcaagacccc ggatgcagat gccggtgaag aataaattta     4680

aatagtgatg cggcctttag gaaacaccac aaaagtaatt gacaatctca ggaacgatct     4740

gcgtgtttac agcttcccaa ataacaatta taccacgtac caaaaggggt ttaatgtatc     4800

tcacaaattc ttctaatagg tacagcttct caaattgggt gtatgatgtg acacttcgtc     4860

tcacacacgt cacgataatt cagcgtatgg cttcccttca tcacattcac gcaaacttct     4920

acacaaccct gggcatattt cttgtgttgg caacactccc gaaatcgatt ctgcacacaa     4980

tggttcattc aatgattcaa tcactccttt gcacgcggtc gggtgctcgg cctacggttg     5040

cccgagtccg caagcacctc aacacagccg tctgtccaca ccgcagccga ccggcgtgcg     5100

atttgggtcc gacccaccgt cccagccccg ctgcggacta tcgcgtcgca gcggccctgc     5160

gcccacgcgg cgtcgtcgaa gttgccgtcg acgagagact ggtaaagctg atcgagtccg     5220

atacgcaaca tataggcgcg gagtcgtggg gagccggcca gctccgggtg cctccgttca     5280

aagtagcgtg tctgctgctc catgcacgcc aaccagggac gccagaagaa tatgttcgcc     5340

acttcgtatt ggctatcacc aaacatcgct tcggaccagt cgatgacagc agtaatccga     5400

ccattgtctg taagtacgtt attgctgccg aaatccgcgt gcaccaggtg cctgacctca     5460

gggcaatcct cggcccacaa catgagttcg tccagtgctt gggccacgga tgcagacacg     5520

gtgtcatcca tgactgtctg ccaatgatag acgtgaggat cggcaatggc gcagatgaag     5580

tctcgccagg tcgtgtactg cccgatgccc tggggcccaa aaggtccaaa gccggacgtc     5640

tgagacagat ctgcggcagc gatcgcgtcc atggcctcgg ccacgggttg caaaacggca     5700

ggcaattcag tttcgggcag atcttgcaac gtcactccct gggctcggcg cgagatgcag     5760

tacgtgagag attcgctaaa ctccccaatg tccagtacct ctggtatggg gagagcggcg     5820

gaggcgaaat gacggtagac ataccgatcc ttgtagaacc cgtccgcaca actattaacc     5880

ctcaacacgt atccccgacc ccctacgtca aacgagaacg ccctactctc ctctccctcg     5940

ctcagttgca tcaagtcgga gacagagtcg aacttctcaa taaggaattt ctccacggac     6000

gtagcggtca gttccggttt cttccccatg atccctgtgt gtctctgtct gtatagaata     6060

ggctaaatta gagaaaagta gcctaaagca gattggagac aggccccgac gcaaatggtg     6120

agttgcttgt ggatcgaacg catacacagg tgtgacgctc catcccgcca ggccatgtta     6180

ttttcatgcc ctcttcccat gtaaagaccg tcccacacat gtcatcattt ctccctgaat     6240

gacaaggcga ataatcacgt agcaatgctt ggccatcgtc gtcgccatgc cgtatcgtcc     6300

agtgcaattt agtcggtgta tggtctctgt gtctccgggt cgcctttctg cgcaatccgc     6360

tcccggaggc ctcgtgagga acccgacagc tcgaaaggag tgaatgcgaa ggcaggatga     6420

ccgagcagaa ttttaaggca gcatctcaac taccacacgg accaatttgg agaagggaca     6480

catgctgcca tctccgtcca aagaaccttg tccgaaatgg ccccgcacct caaggcggag     6540

aattaaaact acattacgcg caacgatttt tttttaaagc tcattggttc atcaattaac     6600

taacagagtc atacagatgc cctggcttaa attaggattc aattgctacg tcgcttgcct     6660

catgtctcag gatgcctgcc aggcagcata ttttcttagt tgtgctttac tcctaagcaa     6720

aatccagcga tactgattca cttcatgaat cgtatgcata cgaccactca ggcctacaaa     6780

gagaagaagt tcatagttgt acgcttacaa atcttaaact tgaacaatct ctttggtttt     6840

gtcggatttc ttcgctttat caaggagtag aatggaagac acagaatcct gttttcccct     6900

acgatgctag acttcacctc aacactccat taaaaaagga tatcacttca ctgttagcat     6960

tttcaacttt aaacattttt cttacagaat tctccggtag cttcgtggct ggctcatctt     7020

tgattatgag gcgcgccagg cattgacgct taagttgact ctgcccaaaa gctgggcgga     7080

gcagccgttg cttcaagtac tggagctgtt catcgaatcc tacaacaaga aaaagaccgg     7140

tctacctccc ttggacaaag actttgtcca catggaaaaa gctgggtaag tccttactcg     7200

tgacagcgtt ccctttctcc agactagacg cctaatagtg ttctaatgta ccactgggac     7260

acgcctcgct gcctgtgcac catgctccat actcaacgct gctacagggg cgtaatcctt     7320

ccagtcggca acattgtgag cgacatgttg agcgatagag atgatttgta tatcagatcc     7380

gggccagggc ctgctcgtgg gaagattgcc catctcagtt cgcccccaaa cgcgcacgct     7440

tcgagtgagt cgagcacagg attgttgcgc tgcaaaaact atggatgcaa tcagtcattt     7500

tcggaagaaa acaattcaga agaggcgtgc cgctttcaca aggcaccccc cgtctttcat     7560

gatacgaaga aagggtggtc gtgctgcgcg aagcgagtat atgactggga cgagttccat     7620

acggtaagcg tggaagtgtt cgttctcggc cccaggactt tgttttgagg caattggtgt     7680

actttaattg gcggataaag ggaggactca caactttcga tattcaccgt ctccagatcg     7740

aggggtgcac cacaggacgg cacagtctca tcgatccgaa ggaaattttc gcgccgtccc     7800

ccaccctggc tgcagccgcg caggccgaga ggggagattg cagcaatacg tcaagcgctg     7860

ctacagtcat caagagcatt gatgaattca atcagtcgaa tccaaatgcc gccgctgcat     7920

gcaaaacagc agcctcgatg acgctggcgg gcacgcgctg caccgtcaaa ccggacgggt     7980

ctgccacctg tttgaacaaa ggctgccaaa aggactactt gctcaaggag aatcacccct     8040

ctgcatgtcg gtaaggacac cgcgctcgat ggaatcgtga gctttacgtt cccacgccaa     8100

cacttcgcca tttctcctcc cttcctttct ttagctacca cgcagccggc cccgtcttcc     8160

acgacgcggg taaatactgg tcatgttgcc ctggaacggt caagtacgac ttcgacgact     8220

ttctcaagat ccctggatgc atgctcagta gtcattacga cggaagccag gagagcctgg     8280

aggcgttcac tagacacgcc aaaacgtctg agggcacatg agaatgtggg atggggagaa     8340

agaaccacgt atccccacga ggaatggcgc attgggagcg agggggatcg acataggaag     8400

aagcaataaa aattctgctc aatacggtat tttttatttt ttggcgtctt ccgttcgtag     8460

ccggcggacg aacagcgatg caaactcgag acggacagtg ccccacggtc ttcgttcgaa     8520

aacatcattg ccaacaacct cgcacactta cttccaggat cctataaata tcttacaacc     8580

accatccgcc tcttccttct cacgcgcatg gataatcaac gccacgattc acaccgttgc     8640

ctggacattt cccccccatg acgggtaatc ctacccgttt ccttccagcg ttccccttct     8700

cttccattcg aacttgaatg cactttactt atttctcttg tgagcatcgt gcatccgctt     8760

gctggcaacg gcaatgggaa tgataatcac aagcaggatc gccaggattg ccagtcccat     8820

aaccattttc atctagatcg tatggagcaa aggaagagag agggggtaag ggcgagaaga     8880

tcgcaatgaa aagggatgac ggcccacgcc gcctcctgcg acttccaatc gtccgaggtt     8940

tacacacatg gcacagccag aaacaccccc ggcataggag caggataggg gtaagacaac     9000

caagcacgta ctcggagctg tttccagcac atctgtttaa acgacgaaag ggcctcgtga     9060

tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca     9120

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata     9180

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga     9240

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc     9300

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg     9360

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc     9420

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat     9480

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact     9540

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat     9600

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga     9660

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc     9720

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga     9780

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag     9840

cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc     9900

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt     9960

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct    10020

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg    10080

cctcactgat taagcattgg taa                                            10103


<210>  28
<211>  2016
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  CHORD target upstream homology arm

<400>  28
agggggcatc agaatctatg ccgtatatga gcttatgaat gtcatccatt atctcatgtc       60

tgtcaacgcg gtcctcgggt gtaacaaaac acaggaggag cgtctcactt ttgaccagca      120

gctttctgcc ttagagcgca ccgtccgtgt caaagaaaat gagctcaaag aattggagct      180

tctatctagt acgtgacatt atataaggag gcaaagtgct ctattaatat gttgacgaga      240

aggtttaata catgcagcag gtaacagatg caggacaagt gtgtgaaatt taaccggcta      300

cttcctatga atcaaacaga agatgcttac aatgcacagg agatcgctga acaagaacta      360

gcaaaagagc gctctgaata taatgaggag cgtgcgcgaa gggaaagaag gctacgggaa      420

cgacagcgta atgcaaaata ttgtttacaa agacaaatgc aagagcagga gaagtcgcaa      480

ggcgaaagtg cgggtggtag catatgccaa caggaaactg accagcagga cgagcaagac      540

gacgaagctg gtagcgaaga ttacgtgact acacagcaag tggaagcgga cagattaaga      600

actttgacaa tgcttgtcaa aagtataact gggatcggtg atgcacaggc attgcgctca      660

aaacttgagc accaagggga ggtcttgcgg aagtcacaag aaattagcaa agataatcag      720

gtgactacaa gaatgataga ctggtactac cccagactcg ttgacatccg gcgtgtcgag      780

cgttcacatc agagatatga aggtagatga cggtaaaagt ttcctaaaat cgagcacgcg      840

actttttttt cttcttcaat atctgagcag gatcgcttag agaagctaca gaaagagcgg      900

gacatcctct ggaatcaatt acagagttcc agatattcac cgcgccccct caaggtctgt      960

aacgaataat aagagaggtc atcacttggc cctgatcgcg atgctgtaga ggttattgat     1020

gaaaggggag gaaagaatgg caggggccgt atagaaataa tcctcaggat tttgaaggcg     1080

tgcgtcatcc tagaccaaat ttgcaggaaa gatgcttaga aatctcaaaa ttgacgcgga     1140

tatttttgac taatcgacaa acatgctttc acgaccacag tccgaaggcc tacttcgcca     1200

gctgttgcac gacaaggaaa aagtcaagga gatcgaaaat aggtgcgagt acttagcaca     1260

acttgtggca catttgcagt gtggtttagt ggagcgttta tggcctttag taatgcgaag     1320

caatcggaat caccttgctg cttcgccaaa gacggaaaat ctcgatcgag tggcgcttca     1380

acgtatgctt aaaaagttag aagtccgctg cttgcagctg cttgacacca aatccatccc     1440

acaaaagcat tgaaattctc aaatatgggg aaccaaacgc tcatccaacc catcccaagc     1500

cccaggcttg cttctgtcga atccgcagca ctcagcacgc atgtgtagta tcttttggat     1560

aaatcattgc aaagaaggtg tataaggctt tttcccggaa gcaaaagctc ccgtgggcac     1620

tgtagaaaca aaagtgaggt gatagttatt tttcgatgca cggaacggta tatatattcg     1680

caaagcttgt ggaggctatc gctgtccttt gtttgaagca gtcttcagcg aagctgccac     1740

ggagcttcgt tcaggaccgt ttcttggcat tcaaaactct acgactatga tactatcgag     1800

cattccgcat gattacagcg caaagcctac caaaaagatg cggtaattca cttgcgggac     1860

cgcaatcatt gtcagccatc agcgcactgt actcgtaaaa aggcgaacat tgtccggtaa     1920

ctcgctcatg tctcacttga gctgatgtcc tctctaaaat aatagggtcg gtcccaaact     1980

cttttaaact ccgatgaaac tctatgtgca ctacga                               2016


<210>  29
<211>  1997
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  CHORD target upstream homology arm

<400>  29
aggcattgac gcttaagttg actctgccca aaagctgggc ggagcagccg ttgcttcaag       60

tactggagct gttcatcgaa tcctacaaca agaaaaagac cggtctacct cccttggaca      120

aagactttgt ccacatggaa aaagctgggt aagtccttac tcgtgacagc gttccctttc      180

tccagactag acgcctaata gtgttctaat gtaccactgg gacacgcctc gctgcctgtg      240

caccatgctc catactcaac gctgctacag gggcgtaatc cttccagtcg gcaacattgt      300

gagcgacatg ttgagcgata gagatgattt gtatatcaga tccgggccag ggcctgctcg      360

tgggaagatt gcccatctca gttcgccccc aaacgcgcac gcttcgagtg agtcgagcac      420

aggattgttg cgctgcaaaa actatggatg caatcagtca ttttcggaag aaaacaattc      480

agaagaggcg tgccgctttc acaaggcacc ccccgtcttt catgatacga agaaagggtg      540

gtcgtgctgc gcgaagcgag tatatgactg ggacgagttc catacggtaa gcgtggaagt      600

gttcgttctc ggccccagga ctttgttttg aggcaattgg tgtactttaa ttggcggata      660

aagggaggac tcacaacttt cgatattcac cgtctccaga tcgaggggtg caccacagga      720

cggcacagtc tcatcgatcc gaaggaaatt ttcgcgccgt cccccaccct ggctgcagcc      780

gcgcaggccg agaggggaga ttgcagcaat acgtcaagcg ctgctacagt catcaagagc      840

attgatgaat tcaatcagtc gaatccaaat gccgccgctg catgcaaaac agcagcctcg      900

atgacgctgg cgggcacgcg ctgcaccgtc aaaccggacg ggtctgccac ctgtttgaac      960

aaaggctgcc aaaaggacta cttgctcaag gagaatcacc cctctgcatg tcggtaagga     1020

caccgcgctc gatggaatcg tgagctttac gttcccacgc caacacttcg ccatttctcc     1080

tcccttcctt tctttagcta ccacgcagcc ggccccgtct tccacgacgc gggtaaatac     1140

tggtcatgtt gccctggaac ggtcaagtac gacttcgacg actttctcaa gatccctgga     1200

tgcatgctca gtagtcatta cgacggaagc caggagagcc tggaggcgtt cactagacac     1260

gccaaaacgt ctgagggcac atgagaatgt gggatgggga gaaagaacca cgtatcccca     1320

cgaggaatgg cgcattggga gcgaggggga tcgacatagg aagaagcaat aaaaattctg     1380

ctcaatacgg tattttttat tttttggcgt cttccgttcg tagccggcgg acgaacagcg     1440

atgcaaactc gagacggaca gtgccccacg gtcttcgttc gaaaacatca ttgccaacaa     1500

cctcgcacac ttacttccag gatcctataa atatcttaca accaccatcc gcctcttcct     1560

tctcacgcgc atggataatc aacgccacga ttcacaccgt tgcctggaca tttccccccc     1620

atgacgggta atcctacccg tttccttcca gcgttcccct tctcttccat tcgaacttga     1680

atgcacttta cttatttctc ttgtgagcat cgtgcatccg cttgctggca acggcaatgg     1740

gaatgataat cacaagcagg atcgccagga ttgccagtcc cataaccatt ttcatctaga     1800

tcgtatggag caaaggaaga gagagggggt aagggcgaga agatcgcaat gaaaagggat     1860

gacggcccac gccgcctcct gcgacttcca atcgtccgag gtttacacac atggcacagc     1920

cagaaacacc cccggcatag gagcaggata ggggtaagac aaccaagcac gtactcggag     1980

ctgtttccag cacatct                                                    1997


<210>  30
<211>  23
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  CHORD-CRISPR target, including PAM sequence

<400>  30
ggaggctggt caggatgaaa agg                                               23


<210>  31
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  20 nucleotide targeting sequence homologous to CHORD gene

<400>  31
ggaggcuggu caggaugaaa                                                   20


<210>  32
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Entire chimeric guide RNA for targeting CHORD gene

<400>  32
ggaggcuggu caggaugaaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu                        103


<210>  33
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo-chord3 sense oligonucleotide

<400>  33
taatacgact cactatagga ggctggtcag gatgaaagtt ttagagctag aaatagcaag       60

ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt      120


<210>  34
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo-chord3 reverse complement oligonucleotide

<400>  34
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aactttcatc ctgaccagcc tcctatagtg agtcgtatta      120


<210>  35
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 Promoter

<400>  35
taatacgact cactatagg                                                    19


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo- KFchord3 Forward primer

<400>  36
gtcggtccca aactctttta aactc                                             25


<210>  37
<211>  25
<212>  DNA
<213>  Synthetic


<220>
<221>  misc_feature
<223>  oligo- KFchord3 Reverse primer

<400>  37
gtcggtccca aactctttta aactc                                             25


<210>  38
<211>  11263
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSGE-6206 vector

<400>  38
gcggccgccg tatggtcgac ggttgctcgg atgggggggg cggggagcga tggagggagg       60

aagatcaggt aaggtctcga cagactagag aagcacgagt gcaggtataa gaaacagcaa      120

aaaaaagtaa tgggcccagg cctggagagg gtatttgtct tgtttttctt tggccaggaa      180

cttgttctcc tttcttcgtt tctaggaccc cgatccccgc tcgcatttct ctcttcctca      240

gccgaagcgc agcggtaaag catccatttt atcccaccga aagggcgctc ccagccttcg      300

tcgagcggaa ccggggttac agtgcctcaa ccctcccaga cgtagccaga gggaagcaac      360

tccctgatgc caaccgctgt gggctgccca tcggaatctt tgacaattgc cttgatcccc      420

gggtgcaagt caagcagcac ctgccgacat cgcccgcacg gagacagaat gccgcggttt      480

tcgttcccga tggccactat gcacgtcaga tttccggcag cagccgcagc ggccgttccg      540

aggaccacga gctccgcgca tggccctccg gtgaaatgat atacattcac gccggtaaag      600

atccgaccgt cggacgagag ggctgcactg gccaccgagt agtcctcgct aataggtatg      660

ctgttgatgg tcgcagttgc acgttcgatc agcgtggatt cctcttggga taaaggcttg      720

gccatcgagc tcggtacccg gggatccatg attgttgtat tatgtaccta tgtttgtgat      780

gagacaataa atatgagaag agaacgttgc ggccactttt ttctccttcc ttcgcgtgct      840

catgttggtg gtttgggagg cagaagatgc atggagcgcc acacattcgg taggacgaaa      900

cagcctcccc cacaaaggga ccatgggtag ctaggatgac gcacaagcga gttcccgctc      960

tcgaagggaa acccaggcat ttccttcctc ttttcaagcc acttgttcac gtgtcaacac     1020

aattttggac taaaatgccc ctcggaactc ggcaggcctc cctctgctcc gttgtcctgg     1080

tcgccgagaa cgcgagaccg tgccgcatgc catcgatctg ctcgtctgta ctactaatcg     1140

tgtgcgtgtt cgtgcttgtt tcgcacgaaa ttgtcctcgt tcggccctca caacggtgga     1200

aatcggtgct agaataaagt gaggtggctt atttcaatgg cggccgtcat catgcgggat     1260

caactgaagt acggcgggtt ctcgagattt catcgtgctc gtccagagca ggtgttttgc     1320

ctgcagctct tcatgtttag gggtcatgat ttcatctgat atgccgtaag aaaaccaata     1380

ttcacttctc aattttccat ggaaaggtga aggcctaggt tgtgtgcgag gcaacgactg     1440

gggagggatc gcaacattct tgctaacctc ccctctatct tggccgctgt gaatcggcat     1500

atttaccggg ctgaattgag aaagtgtttt gagggaatta aaaggtggct gtcttgcaag     1560

cttggcttca gtgcctgctt aattcgaacc gatccagctt gtgatgaggc cttcctaagc     1620

ctggtagtca gaagcgacat ggcgctataa atttcgtctc agttggagag tagaaaagca     1680

tgattcgaac acggttttca actgccaaag atatctccat tgtttccttc aatctgtaca     1740

cctgcacggt gcaccagttg gtacggcata ttatggttta ataagcatac atcatatgaa     1800

tacaattcag cttaaattta tcatacaaag atgtaagtgc agcgtgggtc tgtaacgatc     1860

gggcgtaatt taagataatg cgagggaccg ggggaggttt tggaacggaa tgaggaatgg     1920

gtcatggccc ataataataa tatgggtttg gtcgcctcgc acagcaaccg tacgtgcgaa     1980

aaaggaacag atccatttaa taagttgaac gttattcttt cctatgcaat gcgtgtatcg     2040

gaggcgagag caagtcatag gtggctgcgc acaataattg agtctcagct gagcgccgtc     2100

cgcgggtggt gtgagtggtc atcctcctcc cggcctatcg ctcacatcgc ctctcaatgg     2160

tggtggtggg gcctgatatg acctcaatgc cgacccatat taaaacccag taaagcattc     2220

accaacgaac gaggggctct tttgtgtgtg ttttgagtat gattttacac ctctttgtgc     2280

atctctctgg tcttccttgg ttcccgtagt ttgggcatca tcactcacgc ttccctcgac     2340

cttcgttctt cctttacaac cccgacacag gtcagagttg gagtaatcaa aaaaggggtg     2400

cacgaatgag atacattaga ttttgacaga tatcctttta ctggagaggg ttcaagggat     2460

caaatgaaca gcgggcgttg gcaatctagg gagggatcgg aggttggcag cgagcgaaag     2520

cgtgtccatc cttttggctg tcacacctca cgaaccaact gttagcaggc cagcacagat     2580

gacatacgag aatctttatt atatcgtaga ccttatgtgg atgacctttg gtgctgtgtg     2640

tctggcaatg aacctgaagg cttgataggg aggtggctcc cgtaaaccct ttgtcctttc     2700

cacgctgagt ctcccccgca ctgtccttta tacaaattgt tacagtcatc tgcaggcggt     2760

ttttctttgg caggcaaaga tgcccaagaa aaagcggaag gtcggcgact acaaggatga     2820

cgatgacaag ttggagcctg gagagaagcc ctacaaatgc cctgagtgcg gaaagagctt     2880

cagccaatct ggagccttga cccggcatca acgaacgcat acacgagaca agaagtactc     2940

catcgggctg gacatcggga cgaactccgt gggatgggcc gtgatcacag acgaatacaa     3000

ggtgccttcc aagaagttca aggtgctggg gaacacggac agacactcca tcaagaagaa     3060

cctcatcggg gccttgctct tcgactccgg agaaaccgcc gaagcaacgc gattgaaaag     3120

aaccgccaga agacgataca cacgacggaa gaaccgcatc tgctacctcc aggagatctt     3180

cagcaacgag atggccaagg tggacgactc gttctttcat cgcctggagg agagcttcct     3240

ggtggaggaa gacaagaaac atgagcgcca cccgatcttc gggaacatcg tggacgaagt     3300

ggcctaccac gagaaatacc ccacgatcta ccacttgcgc aagaaactcg tggactccac     3360

ggacaaagcg gacttgcggt tgatctactt ggccttggcc cacatgatca aatttcgggg     3420

ccacttcctg atcgagggcg acttgaatcc cgacaattcc gacgtggaca agctcttcat     3480

ccagctggtg cagacctaca accagctctt cgaggagaac cccatcaatg cctccggagt     3540

ggacgccaaa gccatcttgt ccgcccgatt gtccaaatcc agacgcttgg agaacttgat     3600

cgcacaactt cctggcgaga agaagaacgg cctcttcggc aacttgatcg cgctgtcgct     3660

gggattgacg cctaacttca agtccaactt cgacttggcc gaggacgcca agttgcaact     3720

gtccaaggac acctacgacg acgacctcga caacctgctg gcccaaattg gcgaccaata     3780

cgcggacttg tttttggcgg ccaagaactt gagcgacgcc atcttgttga gcgacatctt     3840

gcgcgtgaat acggagatca ccaaagcccc tttgtccgcc tctatgatca agcggtacga     3900

cgagcaccac caagacttga ccctgttgaa agccctcgtg cggcaacaat tgcccgagaa     3960

gtacaaggag atcttcttcg accagtccaa gaacgggtac gccggctaca tcgacggagg     4020

agcctcccaa gaagagttct acaagttcat caagcccatc ctggagaaga tggacggcac     4080

cgaggagttg ctcgtgaagc tgaaccgcga agacttgttg cgaaaacagc ggacgttcga     4140

caatggcagc atcccccacc aaatccattt gggagagttg cacgccatct tgcgacggca     4200

agaggacttc tacccgttcc tgaaggacaa ccgcgagaaa atcgagaaga tcctgacgtt     4260

cagaatcccc tactacgtgg gacccttggc ccgaggcaat tcccggtttg catggatgac     4320

gcgcaaaagc gaagagacga tcaccccctg gaacttcgaa gaagtggtcg acaaaggagc     4380

atccgcacag agcttcatcg agcgaatgac gaacttcgac aagaacctgc ccaacgagaa     4440

ggtgttgccc aagcattcgc tgctgtacga gtacttcacg gtgtacaacg agctgaccaa     4500

ggtgaagtac gtgaccgagg gcatgcgcaa acccgcgttc ctgtcgggag agcaaaagaa     4560

ggccattgtg gacctgctgt tcaagaccaa ccggaaggtg accgtgaaac agctgaaaga     4620

ggactacttc aagaagatcg agtgcttcga ctccgtggag atctccggcg tggaggaccg     4680

attcaatgcc tccttgggaa cctaccatga cctcctgaag atcatcaagg acaaggactt     4740

cctggacaac gaggagaacg aggacatcct ggaggacatc gtgctgaccc tgaccctgtt     4800

cgaggaccga gagatgatcg aggaacggtt gaaaacgtac gcccacttgt tcgacgacaa     4860

ggtgatgaag cagctgaaac gccgccgcta caccggatgg ggacgattga gccgcaaact     4920

gattaatgga attcgcgaca agcaatccgg aaagaccatc ctggacttcc tgaagtccga     4980

cgggttcgcc aaccgcaact tcatgcagct catccacgac gactccttga ccttcaagga     5040

ggacatccag aaggcccaag tgtccggaca aggagactcc ttgcacgagc acatcgccaa     5100

tttggccgga tcccccgcaa tcaaaaaagg catcttgcaa accgtgaaag tggtcgacga     5160

actggtgaag gtgatgggac ggcacaagcc cgagaacatc gtgatcgaaa tggcccgcga     5220

gaaccaaacc acccaaaaag gacagaagaa ctcccgagag cgcatgaagc ggatcgaaga     5280

gggcatcaag gagttgggct cccagatcct gaaggagcat cccgtggaga atacccaatt     5340

gcaaaacgag aagctctacc tctactacct ccagaacggg cgggacatgt acgtcgacca     5400

agagctggac atcaaccgcc tctccgacta cgatgtggat catattgtgc cccagagctt     5460

cctcaaggac gacagcatcg acaacaaggt cctgacgcgc agcgacaaga accggggcaa     5520

gtctgacaat gtgccttccg aagaagtcgt gaagaagatg aagaactact ggcggcagct     5580

gctcaacgcc aagctcatca cccaacggaa gttcgacaac ctgaccaagg ccgagagagg     5640

aggattgtcc gagttggaca aagccggctt cattaaacgc caactcgtgg agacccgcca     5700

gatcacgaag cacgtggccc aaatcttgga ctcccggatg aacacgaaat acgacgagaa     5760

tgacaagctg atccgcgagg tgaaggtgat cacgctgaag tccaagctgg tgagcgactt     5820

ccggaaggac ttccagttct acaaggtgcg ggagatcaac aactaccatc acgcccatga     5880

cgcctacctg aacgccgtgg tcggaaccgc cctgatcaag aaatacccca agctggagtc     5940

cgaattcgtg tacggagatt acaaggtcta cgacgtgcgg aagatgatcg cgaagtccga     6000

gcaggagatc ggcaaagcca ccgccaagta cttcttttac tccaacatca tgaacttctt     6060

caagaccgag atcacgctcg ccaacggcga gatccgcaag cgccccctga tcgagaccaa     6120

cggcgagacg ggagagattg tgtgggacaa aggaagagat tttgccacag tgcgcaaggt     6180

gctgtccatg cctcaggtga acatcgtgaa gaagaccgag gtgcaaacag gagggttttc     6240

caaagagtcc attttgccta agaggaattc cgacaagctc atcgcccgca agaaggactg     6300

ggaccccaag aagtacgggg gcttcgactc ccccacggtg gcctactccg tgttggtggt     6360

ggccaaagtg gagaaaggga agagcaagaa gctgaaatcc gtgaaggagt tgctcggaat     6420

cacgatcatg gaacgatcgt cgttcgagaa aaaccccatc gacttcctcg aagccaaagg     6480

gtacaaagag gtgaagaagg acctgatcat caagctgccc aagtactccc tgttcgagct     6540

ggagaacggc cgcaagcgga tgctggcctc cgccggggaa ctgcagaaag ggaacgaatt     6600

ggccttgccc tccaaatacg tgaacttcct ctacttggcc tcccattacg aaaagctcaa     6660

aggatcccct gaggacaatg agcagaagca actcttcgtg gaacaacaca agcactacct     6720

ggacgagatc atcgagcaga tcagcgagtt ctccaagcgc gtgatcctcg ccgacgccaa     6780

cctggacaag gtgctctccg cctacaacaa gcaccgcgac aagcctatcc gcgagcaagc     6840

cgagaatatc attcacctgt ttaccctgac gaatttggga gcccctgccg cctttaaata     6900

ctttgacacc accatcgacc gcaaaagata cacctccacc aaggaagtct tggacgccac     6960

cctcatccac cagtccatca cgggcctcta cgagacgcgc atcgacctct cccaattggg     7020

cggcgactaa agtgatgcgg cctttaggaa acaccacaaa agtaattgac aatctcagga     7080

acgatctgcg tgtttacagc ttcccaaata acaattatac cacgtaccaa aaggggttta     7140

atgtatctca caaattcttc taataggtac agcttctcaa attgggtgta tgatgtgaca     7200

cttcgtctca cacacgtcac gataattcag cgtatggctt cccttcatca cattcacgca     7260

aacttctaca caaccctggg catatttctt gtgttggcaa cactcccgaa atcgattctg     7320

cacacaatgg ttcattcaat gattcaagta cgttttagac ggactaggca gtttaattaa     7380

aaacatctat cctccagatc accagggcca gtgaggccgg cataaaggac ggcaaggaaa     7440

gaaaagaaag aaagaaaagg acacttatag catagtttga agttataagt agtcgcaatc     7500

tgtgtgcagc cgacagatgc tttttttttc cgtttggcag gaggtgtagg gatgtcgaag     7560

accagtccag ctagtatcta tcctacaagt caatcatgct gcgacaaaaa tttctcgcac     7620

gaggcctctc gataaacaaa actttaaaag cacacttcat tgtcatgcag agtaataact     7680

cttccgcgtc gatcaattta tcaatctcta tcatttccgc ccctttcctt gcatagagca     7740

agaaaagcga cccggatgag gataacatgt cctgcgccag tagtgtggca ttgcctgtct     7800

ctcatttaca cgtactgaaa gcataatgca cgcgcatacc aatatttttc gtgtacggag     7860

atgaagagac gcgacacgta agatcacgag aaggcgagca cggttgccaa tggcagacgc     7920

gctagtctcc attatcgcgt tgttcggtag cttgctgcat gtcttcagtg gcactatatc     7980

cactctgcct cgtcttctac acgagggcca catcggtgca agttcgaaaa atcatatctc     8040

aatcttcaga tcctttccag aaacggtgct caggcgggaa agtgaaggtt ttctactcta     8100

gtggctaccc caattctctc cgactgtcgc agacggtcct tcgttgcgca cgcaccgcgc     8160

actacctctg aaattcgaca accgaagttc aattttacat ctaacttctt tcccattctc     8220

tcaccaaaag cctagcttac atgttggaga gcgacgagag cggcctgccc gccatggaga     8280

tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag     8340

agggcacccc cgagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga     8400

ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct     8460

accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca     8520

cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg     8580

aggccggccg cgtgatcggc gacttcaagg tgatgggcac cggcttcccc gaggacagcg     8640

tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg     8700

gcgataacga tctggatggc agcttcaccc gcaccttcag cctgcgcgac ggcggctact     8760

acagctccgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc     8820

agaacggggg ccccatgttc gccttccgcc gcgtggagga ggatcacagc aacaccgagc     8880

tgggcatcgt ggagtaccag cacgccttca agaccccgga tgcagatgcc ggtgaagaat     8940

aagggtggga aggagtcggg gagggtcctg gcagagcggc gtcctcatga tgtgttggag     9000

acctggagag tcgagagctt cctcgtcacc tgattgtcat gtgtgtatag gttaaggggg     9060

cccactcaaa gccataaaga cgaacacaaa cactaatctc aacaaagtct actagcatgc     9120

cgtctgtcca tctttatttc ctggcgcgcc tatgcttgta aaccgttttg tgaaaaaatt     9180

tttaaaataa aaaaggggac ctctagggtc cccaattaat tagtaatata atctattaaa     9240

ggtcattcaa aaggtcatcc agacgaaagg gcctcgtgat acgcctattt ttataggtta     9300

atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg     9360

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     9420

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     9480

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     9540

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     9600

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     9660

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag     9720

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     9780

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     9840

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     9900

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     9960

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa    10020

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag    10080

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct    10140

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac    10200

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa    10260

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt    10320

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat    10380

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg    10440

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc    10500

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg    10560

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag    10620

cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact    10680

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg    10740

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc    10800

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg    10860

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg    10920

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag    10980

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc    11040

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct    11100

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc    11160

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc    11220

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga                      11263


<210>  39
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes N-terminal peptide linker

<400>  39
atgcccaaga aaaagcggaa ggtcggcgac tacaaggatg acgatgacaa gttggagcct       60

ggagagaagc cctacaaatg ccctgagtgc ggaaagagct tcagccaatc tggagccttg      120

acccggcatc aacgaacgca tacacga                                          147


<210>  40
<211>  822
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  4A-III promoter

<400>  40
ggcataaagg acggcaagga aagaaaagaa agaaagaaaa ggacacttat agcatagttt       60

gaagttataa gtagtcgcaa tctgtgtgca gccgacagat gctttttttt tccgtttggc      120

aggaggtgta gggatgtcga agaccagtcc agctagtatc tatcctacaa gtcaatcatg      180

ctgcgacaaa aatttctcgc acgaggcctc tcgataaaca aaactttaaa agcacacttc      240

attgtcatgc agagtaataa ctcttccgcg tcgatcaatt tatcaatctc tatcatttcc      300

gcccctttcc ttgcatagag caagaaaagc gacccggatg aggataacat gtcctgcgcc      360

agtagtgtgg cattgcctgt ctctcattta cacgtactga aagcataatg cacgcgcata      420

ccaatatttt tcgtgtacgg agatgaagag acgcgacacg taagatcacg agaaggcgag      480

cacggttgcc aatggcagac gcgctagtct ccattatcgc gttgttcggt agcttgctgc      540

atgtcttcag tggcactata tccactctgc ctcgtcttct acacgagggc cacatcggtg      600

caagttcgaa aaatcatatc tcaatcttca gatcctttcc agaaacggtg ctcaggcggg      660

aaagtgaagg ttttctactc tagtggctac cccaattctc tccgactgtc gcagacggtc      720

cttcgttgcg cacgcaccgc gcactacctc tgaaattcga caaccgaagt tcaattttac      780

atctaacttc tttcccattc tctcaccaaa agcctagctt ac                         822


<210>  41
<211>  200
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  bidirectional terminator 5

<400>  41
gggtgggaag gagtcgggga gggtcctggc agagcggcgt cctcatgatg tgttggagac       60

ctggagagtc gagagcttcc tcgtcacctg attgtcatgt gtgtataggt taagggggcc      120

cactcaaagc cataaagacg aacacaaaca ctaatctcaa caaagtctac tagcatgccg      180

tctgtccatc tttatttcct                                                  200


<210>  42
<211>  213
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  6487 terminator

<400>  42
tgagatgcgg aactcgcatc ttatattgca atttgatata cacagacatg acttggaaag       60

taaaagcaag ctgggtatcg gcgttacaag aaataccagg cctctggtga tgtattacac      120

tcaaaaaatt aaaaggtggt agagacaatc atgcatatgc attgtctttt gcctaatgag      180

gaaatttcga ggtgttcttc agaaaaaatc tta                                   213


<210>  43
<211>  27
<212>  DNA
<213>  Synthetic


<220>
<221>  misc_feature
<223>  5'ID sequence

<400>  43
tccacagccc gaacccatga gagagaa                                           27


<210>  44
<211>  27
<212>  DNA
<213>  Synthetic


<220>
<221>  misc_feature
<223>  3'ID sequence

<400>  44
gcccgaatcg agttgatggc ccgcaaa                                           27


<210>  45
<211>  2346
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HygR Cassette

<400>  45
tcataatcaa agatgagcca gccacgaagc taccggagaa ttctgtaaga aaaatgttta       60

aagttgaaaa tgctaacagt gaagtgatat ccttttttaa tggagtgttg aggtgaagtc      120

tagcatcgta ggggaaaaca ggattctgtg tcttccattc tactccttga taaagcgaag      180

aaatccgaca aaaccaaaga gattgttcaa gtttaagatt tgtaagcgta caactatgaa      240

cttcttctct ttgtaggcct gagtggtcgt atgcatacga ttcatgaagt gaatcagtat      300

cgctggattt tgcttaggag taaagcacaa ctaagaaaat atgctgcctg gcaggcatcc      360

tgagacatga ggcaagcgac gtagcaattg aatcctaatt taagccaggg catctgtatg      420

actctgttag ttaattgatg aaccaatgag ctttaaaaaa aaatcgttgc gcgtaatgta      480

gttttaattc tccgccttga ggtgcggggc catttcggac aaggttcttt ggacggagat      540

ggcagcatgt gtcccttctc caaattggtc cgtgtggtag ttgagatgct gccttaaaat      600

tctgctcggt catcctgcct tcgcattcac tcctttcgag ctgtcgggtt cctcacgagg      660

cctccgggag cggattgcgc agaaaggcga cccggagaca cagagaccat acaccgacta      720

aattgcactg gacgatacgg catggcgacg acgatggcca agcattgcta cgtgattatt      780

cgccttgtca ttcagggaga aatgatgaca tgtgtgggac ggtctttaca tgggaagagg      840

gcatgaaaat aacatggcct ggcgggatgg agcgtcacac ctgtgtatgc gttcgatcca      900

caagcaactc accatttgcg tcggggcctg tctccaatct gctttaggct acttttctct      960

aatttagcct attctataca gacagagaca cacagggatc atggggaaga aaccggaact     1020

gaccgctacg tccgtggaga aattccttat tgagaagttc gactctgtct ccgacttgat     1080

gcaactgagc gagggagagg agagtagggc gttctcgttt gacgtagggg gtcggggata     1140

cgtgttgagg gttaatagtt gtgcggacgg gttctacaag gatcggtatg tctaccgtca     1200

tttcgcctcc gccgctctcc ccataccaga ggtactggac attggggagt ttagcgaatc     1260

tctcacgtac tgcatctcgc gccgagccca gggagtgacg ttgcaagatc tgcccgaaac     1320

tgaattgcct gccgttttgc aacccgtggc cgaggccatg gacgcgatcg ctgccgcaga     1380

tctgtctcag acgtccggct ttggaccttt tgggccccag ggcatcgggc agtacacgac     1440

ctggcgagac ttcatctgcg ccattgccga tcctcacgtc tatcattggc agacagtcat     1500

ggatgacacc gtgtctgcat ccgtggccca agcactggac gaactcatgt tgtgggccga     1560

ggattgccct gaggtcaggc acctggtgca cgcggatttc ggcagcaata acgtacttac     1620

agacaatggt cggattactg ctgtcatcga ctggtccgaa gcgatgtttg gtgatagcca     1680

atacgaagtg gcgaacatat tcttctggcg tccctggttg gcgtgcatgg agcagcagac     1740

acgctacttt gaacggaggc acccggagct ggccggctcc ccacgactcc gcgcctatat     1800

gttgcgtatc ggactcgatc agctttacca gtctctcgtc gacggcaact tcgacgacgc     1860

cgcgtgggcg cagggccgct gcgacgcgat agtccgcagc ggggctggga cggtgggtcg     1920

gacccaaatc gcacgccggt cggctgcggt gtggacagac ggctgtgttg aggtgcttgc     1980

ggactcgggc aaccgtaggc cgagcacccg accgcgtgca aaggagtgat tgaatcattg     2040

aatgaaccat tgtgtgcaga atcgatttcg ggagtgttgc caacacaaga aatatgccca     2100

gggttgtgta gaagtttgcg tgaatgtgat gaagggaagc catacgctga attatcgtga     2160

cgtgtgtgag acgaagtgtc acatcataca cccaatttga gaagctgtac ctattagaag     2220

aatttgtgag atacattaaa ccccttttgg tacgtggtat aattgttatt tgggaagctg     2280

taaacacgca gatcgttcct gagattgtca attacttttg tggtgtttcc taaaggccgc     2340

atcact                                                                2346


<210>  46
<211>  2400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HygR Cassette with flanking ID sequences

<400>  46
tccacagccc gaacccatga gagagaatca taatcaaaga tgagccagcc acgaagctac       60

cggagaattc tgtaagaaaa atgtttaaag ttgaaaatgc taacagtgaa gtgatatcct      120

tttttaatgg agtgttgagg tgaagtctag catcgtaggg gaaaacagga ttctgtgtct      180

tccattctac tccttgataa agcgaagaaa tccgacaaaa ccaaagagat tgttcaagtt      240

taagatttgt aagcgtacaa ctatgaactt cttctctttg taggcctgag tggtcgtatg      300

catacgattc atgaagtgaa tcagtatcgc tggattttgc ttaggagtaa agcacaacta      360

agaaaatatg ctgcctggca ggcatcctga gacatgaggc aagcgacgta gcaattgaat      420

cctaatttaa gccagggcat ctgtatgact ctgttagtta attgatgaac caatgagctt      480

taaaaaaaaa tcgttgcgcg taatgtagtt ttaattctcc gccttgaggt gcggggccat      540

ttcggacaag gttctttgga cggagatggc agcatgtgtc ccttctccaa attggtccgt      600

gtggtagttg agatgctgcc ttaaaattct gctcggtcat cctgccttcg cattcactcc      660

tttcgagctg tcgggttcct cacgaggcct ccgggagcgg attgcgcaga aaggcgaccc      720

ggagacacag agaccataca ccgactaaat tgcactggac gatacggcat ggcgacgacg      780

atggccaagc attgctacgt gattattcgc cttgtcattc agggagaaat gatgacatgt      840

gtgggacggt ctttacatgg gaagagggca tgaaaataac atggcctggc gggatggagc      900

gtcacacctg tgtatgcgtt cgatccacaa gcaactcacc atttgcgtcg gggcctgtct      960

ccaatctgct ttaggctact tttctctaat ttagcctatt ctatacagac agagacacac     1020

agggatcatg gggaagaaac cggaactgac cgctacgtcc gtggagaaat tccttattga     1080

gaagttcgac tctgtctccg acttgatgca actgagcgag ggagaggaga gtagggcgtt     1140

ctcgtttgac gtagggggtc ggggatacgt gttgagggtt aatagttgtg cggacgggtt     1200

ctacaaggat cggtatgtct accgtcattt cgcctccgcc gctctcccca taccagaggt     1260

actggacatt ggggagttta gcgaatctct cacgtactgc atctcgcgcc gagcccaggg     1320

agtgacgttg caagatctgc ccgaaactga attgcctgcc gttttgcaac ccgtggccga     1380

ggccatggac gcgatcgctg ccgcagatct gtctcagacg tccggctttg gaccttttgg     1440

gccccagggc atcgggcagt acacgacctg gcgagacttc atctgcgcca ttgccgatcc     1500

tcacgtctat cattggcaga cagtcatgga tgacaccgtg tctgcatccg tggcccaagc     1560

actggacgaa ctcatgttgt gggccgagga ttgccctgag gtcaggcacc tggtgcacgc     1620

ggatttcggc agcaataacg tacttacaga caatggtcgg attactgctg tcatcgactg     1680

gtccgaagcg atgtttggtg atagccaata cgaagtggcg aacatattct tctggcgtcc     1740

ctggttggcg tgcatggagc agcagacacg ctactttgaa cggaggcacc cggagctggc     1800

cggctcccca cgactccgcg cctatatgtt gcgtatcgga ctcgatcagc tttaccagtc     1860

tctcgtcgac ggcaacttcg acgacgccgc gtgggcgcag ggccgctgcg acgcgatagt     1920

ccgcagcggg gctgggacgg tgggtcggac ccaaatcgca cgccggtcgg ctgcggtgtg     1980

gacagacggc tgtgttgagg tgcttgcgga ctcgggcaac cgtaggccga gcacccgacc     2040

gcgtgcaaag gagtgattga atcattgaat gaaccattgt gtgcagaatc gatttcggga     2100

gtgttgccaa cacaagaaat atgcccaggg ttgtgtagaa gtttgcgtga atgtgatgaa     2160

gggaagccat acgctgaatt atcgtgacgt gtgtgagacg aagtgtcaca tcatacaccc     2220

aatttgagaa gctgtaccta ttagaagaat ttgtgagata cattaaaccc cttttggtac     2280

gtggtataat tgttatttgg gaagctgtaa acacgcagat cgttcctgag attgtcaatt     2340

acttttgtgg tgtttcctaa aggccgcatc actgcccgaa tcgagttgat ggcccgcaaa     2400


<210>  47
<211>  8393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSGE-6281

<400>  47
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt       60

aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag      120

ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct      180

ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt      240

tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg      300

cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct      360

gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc      420

gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg      480

tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa      540

ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg      600

gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg      660

ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga      720

tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt      780

ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct      840

gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga      900

acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agtttaaaca gggggcatca      960

gaatctatgc cgtatatgag cttatgaatg tcatccatta tctcatgtct gtcaacgcgg     1020

tcctcgggtg taacaaaaca caggaggagc gtctcacttt tgaccagcag ctttctgcct     1080

tagagcgcac cgtccgtgtc aaagaaaatg agctcaaaga attggagctt ctatctagta     1140

cgtgacatta tataaggagg caaagtgctc tattaatatg ttgacgagaa ggtttaatac     1200

atgcagcagg taacagatgc aggacaagtg tgtgaaattt aaccggctac ttcctatgaa     1260

tcaaacagaa gatgcttaca atgcacagga gatcgctgaa caagaactag caaaagagcg     1320

ctctgaatat aatgaggagc gtgcgcgaag ggaaagaagg ctacgggaac gacagcgtaa     1380

tgcaaaatat tgtttacaaa gacaaatgca agagcaggag aagtcgcaag gcgaaagtgc     1440

gggtggtagc atatgccaac aggaaactga ccagcaggac gagcaagacg acgaagctgg     1500

tagcgaagat tacgtgacta cacagcaagt ggaagcggac agattaagaa ctttgacaat     1560

gcttgtcaaa agtataactg ggatcggtga tgcacaggca ttgcgctcaa aacttgagca     1620

ccaaggggag gtcttgcgga agtcacaaga aattagcaaa gataatcagg tgactacaag     1680

aatgatagac tggtactacc ccagactcgt tgacatccgg cgtgtcgagc gttcacatca     1740

gagatatgaa ggtagatgac ggtaaaagtt tcctaaaatc gagcacgcga cttttttttc     1800

ttcttcaata tctgagcagg atcgcttaga gaagctacag aaagagcggg acatcctctg     1860

gaatcaatta cagagttcca gatattcacc gcgccccctc aaggtctgta acgaataata     1920

agagaggtca tcacttggcc ctgatcgcga tgctgtagag gttattgatg aaaggggagg     1980

aaagaatggc aggggccgta tagaaataat cctcaggatt ttgaaggcgt gcgtcatcct     2040

agaccaaatt tgcaggaaag atgcttagaa atctcaaaat tgacgcggat atttttgact     2100

aatcgacaaa catgctttca cgaccacagt ccgaaggcct acttcgccag ctgttgcacg     2160

acaaggaaaa agtcaaggag atcgaaaata ggtgcgagta cttagcacaa cttgtggcac     2220

atttgcagtg tggtttagtg gagcgtttat ggcctttagt aatgcgaagc aatcggaatc     2280

accttgctgc ttcgccaaag acggaaaatc tcgatcgagt ggcgcttcaa cgtatgctta     2340

aaaagttaga agtccgctgc ttgcagctgc ttgacaccaa atccatccca caaaagcatt     2400

gaaattctca aatatgggga accaaacgct catccaaccc atcccaagcc ccaggcttgc     2460

ttctgtcgaa tccgcagcac tcagcacgca tgtgtagtat cttttggata aatcattgca     2520

aagaaggtgt ataaggcttt ttcccggaag caaaagctcc cgtgggcact gtagaaacaa     2580

aagtgaggtg atagttattt ttcgatgcac ggaacggtat atatattcgc aaagcttgtg     2640

gaggctatcg ctgtcctttg tttgaagcag tcttcagcga agctgccacg gagcttcgtt     2700

caggaccgtt tcttggcatt caaaactcta cgactatgat actatcgagc attccgcatg     2760

attacagcgc aaagcctacc aaaaagatgc ggtaattcac ttgcgggacc gcaatcattg     2820

tcagccatca gcgcactgta ctcgtaaaaa ggcgaacatt gtccggtaac tcgctcatgt     2880

ctcacttgag ctgatgtcct ctctaaaata atagggtcgg tcccaaactc ttttaaactc     2940

cgatgaaact ctatgtgcac tacgagcggc cgcagtgatg cggcctttag gaaacaccac     3000

aaaagtaatt gacaatctca ggaacgatct gcgtgtttac agcttcccaa ataacaatta     3060

taccacgtac caaaaggggt ttaatgtatc tcacaaattc ttctaatagg tacagcttct     3120

caaattgggt gtatgatgtg acacttcgtc tcacacacgt cacgataatt cagcgtatgg     3180

cttcccttca tcacattcac gcaaacttct acacaaccct gggcatattt cttgtgttgg     3240

caacactccc gaaatcgatt ctgcacacaa tggttcattc aatgattcaa tcactccttt     3300

gcacgcggtc gggtgctcgg cctacggttg cccgagtccg caagcacctc aacacagccg     3360

tctgtccaca ccgcagccga ccggcgtgcg atttgggtcc gacccaccgt cccagccccg     3420

ctgcggacta tcgcgtcgca gcggccctgc gcccacgcgg cgtcgtcgaa gttgccgtcg     3480

acgagagact ggtaaagctg atcgagtccg atacgcaaca tataggcgcg gagtcgtggg     3540

gagccggcca gctccgggtg cctccgttca aagtagcgtg tctgctgctc catgcacgcc     3600

aaccagggac gccagaagaa tatgttcgcc acttcgtatt ggctatcacc aaacatcgct     3660

tcggaccagt cgatgacagc agtaatccga ccattgtctg taagtacgtt attgctgccg     3720

aaatccgcgt gcaccaggtg cctgacctca gggcaatcct cggcccacaa catgagttcg     3780

tccagtgctt gggccacgga tgcagacacg gtgtcatcca tgactgtctg ccaatgatag     3840

acgtgaggat cggcaatggc gcagatgaag tctcgccagg tcgtgtactg cccgatgccc     3900

tggggcccaa aaggtccaaa gccggacgtc tgagacagat ctgcggcagc gatcgcgtcc     3960

atggcctcgg ccacgggttg caaaacggca ggcaattcag tttcgggcag atcttgcaac     4020

gtcactccct gggctcggcg cgagatgcag tacgtgagag attcgctaaa ctccccaatg     4080

tccagtacct ctggtatggg gagagcggcg gaggcgaaat gacggtagac ataccgatcc     4140

ttgtagaacc cgtccgcaca actattaacc ctcaacacgt atccccgacc ccctacgtca     4200

aacgagaacg ccctactctc ctctccctcg ctcagttgca tcaagtcgga gacagagtcg     4260

aacttctcaa taaggaattt ctccacggac gtagcggtca gttccggttt cttccccatg     4320

atccctgtgt gtctctgtct gtatagaata ggctaaatta gagaaaagta gcctaaagca     4380

gattggagac aggccccgac gcaaatggtg agttgcttgt ggatcgaacg catacacagg     4440

tgtgacgctc catcccgcca ggccatgtta ttttcatgcc ctcttcccat gtaaagaccg     4500

tcccacacat gtcatcattt ctccctgaat gacaaggcga ataatcacgt agcaatgctt     4560

ggccatcgtc gtcgccatgc cgtatcgtcc agtgcaattt agtcggtgta tggtctctgt     4620

gtctccgggt cgcctttctg cgcaatccgc tcccggaggc ctcgtgagga acccgacagc     4680

tcgaaaggag tgaatgcgaa ggcaggatga ccgagcagaa ttttaaggca gcatctcaac     4740

taccacacgg accaatttgg agaagggaca catgctgcca tctccgtcca aagaaccttg     4800

tccgaaatgg ccccgcacct caaggcggag aattaaaact acattacgcg caacgatttt     4860

tttttaaagc tcattggttc atcaattaac taacagagtc atacagatgc cctggcttaa     4920

attaggattc aattgctacg tcgcttgcct catgtctcag gatgcctgcc aggcagcata     4980

ttttcttagt tgtgctttac tcctaagcaa aatccagcga tactgattca cttcatgaat     5040

cgtatgcata cgaccactca ggcctacaaa gagaagaagt tcatagttgt acgcttacaa     5100

atcttaaact tgaacaatct ctttggtttt gtcggatttc ttcgctttat caaggagtag     5160

aatggaagac acagaatcct gttttcccct acgatgctag acttcacctc aacactccat     5220

taaaaaagga tatcacttca ctgttagcat tttcaacttt aaacattttt cttacagaat     5280

tctccggtag cttcgtggct ggctcatctt tgattatgag gcgcgccagg cattgacgct     5340

taagttgact ctgcccaaaa gctgggcgga gcagccgttg cttcaagtac tggagctgtt     5400

catcgaatcc tacaacaaga aaaagaccgg tctacctccc ttggacaaag actttgtcca     5460

catggaaaaa gctgggtaag tccttactcg tgacagcgtt ccctttctcc agactagacg     5520

cctaatagtg ttctaatgta ccactgggac acgcctcgct gcctgtgcac catgctccat     5580

actcaacgct gctacagggg cgtaatcctt ccagtcggca acattgtgag cgacatgttg     5640

agcgatagag atgatttgta tatcagatcc gggccagggc ctgctcgtgg gaagattgcc     5700

catctcagtt cgcccccaaa cgcgcacgct tcgagtgagt cgagcacagg attgttgcgc     5760

tgcaaaaact atggatgcaa tcagtcattt tcggaagaaa acaattcaga agaggcgtgc     5820

cgctttcaca aggcaccccc cgtctttcat gatacgaaga aagggtggtc gtgctgcgcg     5880

aagcgagtat atgactggga cgagttccat acggtaagcg tggaagtgtt cgttctcggc     5940

cccaggactt tgttttgagg caattggtgt actttaattg gcggataaag ggaggactca     6000

caactttcga tattcaccgt ctccagatcg aggggtgcac cacaggacgg cacagtctca     6060

tcgatccgaa ggaaattttc gcgccgtccc ccaccctggc tgcagccgcg caggccgaga     6120

ggggagattg cagcaatacg tcaagcgctg ctacagtcat caagagcatt gatgaattca     6180

atcagtcgaa tccaaatgcc gccgctgcat gcaaaacagc agcctcgatg acgctggcgg     6240

gcacgcgctg caccgtcaaa ccggacgggt ctgccacctg tttgaacaaa ggctgccaaa     6300

aggactactt gctcaaggag aatcacccct ctgcatgtcg gtaaggacac cgcgctcgat     6360

ggaatcgtga gctttacgtt cccacgccaa cacttcgcca tttctcctcc cttcctttct     6420

ttagctacca cgcagccggc cccgtcttcc acgacgcggg taaatactgg tcatgttgcc     6480

ctggaacggt caagtacgac ttcgacgact ttctcaagat ccctggatgc atgctcagta     6540

gtcattacga cggaagccag gagagcctgg aggcgttcac tagacacgcc aaaacgtctg     6600

agggcacatg agaatgtggg atggggagaa agaaccacgt atccccacga ggaatggcgc     6660

attgggagcg agggggatcg acataggaag aagcaataaa aattctgctc aatacggtat     6720

tttttatttt ttggcgtctt ccgttcgtag ccggcggacg aacagcgatg caaactcgag     6780

acggacagtg ccccacggtc ttcgttcgaa aacatcattg ccaacaacct cgcacactta     6840

cttccaggat cctataaata tcttacaacc accatccgcc tcttccttct cacgcgcatg     6900

gataatcaac gccacgattc acaccgttgc ctggacattt cccccccatg acgggtaatc     6960

ctacccgttt ccttccagcg ttccccttct cttccattcg aacttgaatg cactttactt     7020

atttctcttg tgagcatcgt gcatccgctt gctggcaacg gcaatgggaa tgataatcac     7080

aagcaggatc gccaggattg ccagtcccat aaccattttc atctagatcg tatggagcaa     7140

aggaagagag agggggtaag ggcgagaaga tcgcaatgaa aagggatgac ggcccacgcc     7200

gcctcctgcg acttccaatc gtccgaggtt tacacacatg gcacagccag aaacaccccc     7260

ggcataggag caggataggg gtaagacaac caagcacgta ctcggagctg tttccagcac     7320

atctgtttaa acgacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga     7380

taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     7440

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     7500

aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc     7560

ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga     7620

aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca     7680

acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt     7740

ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg     7800

gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc     7860

atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata     7920

acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt     7980

tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag     8040

ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca     8100

aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg     8160

aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg     8220

ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag     8280

atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg     8340

aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taa            8393


<210>  48
<211>  5712
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  acyl-CoA oxidase gene, genomic sequence

<400>  48
atgacgaccg ccaatgcccg tttgtcgagg ctcaaagatc atttagcaga gacgggggct       60

gtggcgcgcg cggtacgtgt ggagtcttgc tttcttcagg gggggtcccg gggggggggc      120

gagtcagaca aagggtagag cgtactggaa ggacagacag atgctggcat gaccctgcat      180

ccagaacaca catttaataa ttgagggtct ggtgatcgag gggctcggca accgcgcggc      240

tgctagcacg tgacacggtt ttttacctct actgttccac aatcaacccc tacttcccct      300

tctccctccc tctttccctc cctcccctac gttaattttc cctcccgctg tactatatcg      360

cttcactttc agccgattag ctcctctgcc atcaatgcca cgccttttgc ggcgaggacg      420

acgcatacca tggagcgcat ggcaagggaa cgagccaagg cctccttccc cgtccgagac      480

atgacgtact tcttggacgg cgggaggagc atgaccgagg tcaaggtggg tgaaataaaa      540

acataaaaag agaataaaca acgaaaggcg aggtctttgg gggatgcctg aatacgtagg      600

caaccgtact gatcgttttg gacccttctt ctcttggggc cgatggccca caggagggca      660

tgatggcgga cttggcggcg aatccggtct ttacggaccc agaatggaac gacttgaaca      720

gagatcaggt agggatatgg gtgattggcc ggaaggtggg gaaggagggg gaggtgcccc      780

ttgtgagctt gccctgtggt cggagcgctt ccctcgatca acccctccct ctcccttccc      840

cttcccccct ctggctagat ccgtgaacgc accatctctc gactgagagc tgcgtacaag      900

ctcctgatcc gagacggtgc cgatgtcagc cgccggaatg cccggcttga gattcacgcc      960

ctccatgact tgggtagccc ttcctccctc cctccctccc tccctccctt gctccctacc     1020

tcctccccgt gtcctcacct tcgttttttc caagcattta ttacttttca gtttcatgtg     1080

gacgatcatc aaaatgatgc cttcctcgcc cgacgcgatt ggtcctccct ccctccctct     1140

ttctctccct ccctccctcc ctccctccct ttgcagggtg gtacgtgcgg cagggtgtgc     1200

atttcggcct ctttatgggc gccttggccg ggcaggggag cgacgaacaa cgcgctgagt     1260

ggctgcccag gaccatgatg tgtgaggtag gagggaggga gggagggagg gagggagggg     1320

aaggacaaac aggctggtgg aaaccacttg caggaaggtt tgggaatgta cttcggtctg     1380

tccaatccgc catacagcat tttcattcct cccctccctc cctccccccc ctccttcctg     1440

ctctagatct acgggtgctt cgggatgacg gagttggggc acggctcatt cttgcggggc     1500

ctggagacca cagcgatgta cgacaaggta ggtacggagc gagggaggga gcgagggagg     1560

gagcaaggga gggagggagc gagggaggga gggagacagg gaccgacgga gggacgctac     1620

cgaccggttg tggttgttct ctcctttccg tacaggacac gcaagaattt gtaatcaatt     1680

cccccactga cacaagcacc aaatggtagg tgccctccct ccctccctcc ctccctcgcc     1740

tccctgtccc ctgcgaccat ccatccttcc tccctccctc cctccctccc tccctccagg     1800

tggatcggtg cggccgggca gacggccaca cattcggtgg ttttcgcccg cctcctcctt     1860

ccctcagggg acgacatggg tgtgcacaac ttcatcatac ccctccggga tatggaaacg     1920

cacttgcccc tccctggcat ccacattggt acctacctcc ctccctacct gcctccctcc     1980

ctccctcccg ccctgctcat tcctctggct ccctctctaa ttgtcgaaaa aaatatgcga     2040

agacttgtga caacgtcgag ccgcacgctt acttcctctc ctcctccccc tcctcccctc     2100

cttcccgccc tccttcctcc cctccttcct cccctccctt cctcgccctc cttcctcccc     2160

ctccgttccc cctcttcctc cccctccgtt cccccctccc caggcgattt gggggccaag     2220

atgggcttga atggcatcga caacgggtgg atgcaatttg accacgtccg cgtgccccgg     2280

gacaacatgc tttgtcgcta cgcacaggtg cccctccctc cctccctccc tccctccctc     2340

cctcttttcc tccctccctc cctacctcct tcctcctttc tcgctttgtg acacacacac     2400

accctttcgg gtctcccccc cgcgctcagc tccctgcctt ctctccctcc ctccctccct     2460

ccctcgatcc ctccctccct ccctccctcc ctccctcccc ccctcccagg tcaccccgga     2520

ggggaaatac atccgtcctc ccaggaagga gatggcttac ggcgctctca tcggcactcg     2580

ggcggctctg gtcaagacag ccgtggactt tcaaaaaaag gtgggtcctc cctccctccc     2640

tccctccctc cctccctccc tccctccctt ctttttcctg ttgcatgctt tctactcttg     2700

tcctctgtta tttgtgcctt tttcccctcc ctctctccct ccctccctcc tttcactgac     2760

aacgacggcg tgtgtaccga ccacccttcc ctccctccct ccctccctcc ctccctccct     2820

ccctccccca ggccctcatg atcgggatcc gctacaccgc cctccggaca cagggcgtgg     2880

tggaggaagg ccaaagggaa gagaccgcca tcatcgacta ccccatccac cgggacaaac     2940

tcctgaaact cttggcaagc cccgccctcc ctccctccct ccctccctcc ctccctcttt     3000

cccgtcctgc cgctccacgc cacggcctgc tactgacccc caccacctcc tcctccctcc     3060

ctccctccct ccctccctca ggcggccgcc tacgcctggc acttccaagc cgcctacgtt     3120

ctccacctga acgattcctt ggaggagggg ctcgaggcgg gggacctctc catcctcaag     3180

gatgtgcatg ggaccatggc tggtaccccc cgccctccct ccctccctcc ctccctccct     3240

ccctgtttcc cgccctgtgg ggcctcgcag ccttttgcat ccgcttcccc gtgacgctgc     3300

cctccctccc tccctcgctc cctccctccc gccctccctc caggcctcaa ggctttcgga     3360

acctggttca cgtacaacac gatcgaggcc tgccggcaag tgtgcggggg ccacgggtac     3420

agcaagtaca acggcctctc caacaccctc caggtccctc cttccctccc tccctccctc     3480

cctccctccc tcccttcctc cctttcctcc tttcttgtcg tatgatcgga gcgacgcact     3540

gcgtgacgcg ttcctacttc cttgtggtgc tgccgatttc atccctacct ccctccttcc     3600

ctccctccct tcctccctcc cttcctccct ccctcaggac tttgctgtca tgtgcacctg     3660

ggagggcgac aacaccgtga tggctctaca gacggcgcgg tatctagttc ggtcctacga     3720

gaaggcgaag cgggggggcg gtagggaggg agggagggag ggagggaggg agggagggag     3780

ggaaagagga ggaaagaaac ggccaaagaa aggagaacat ccattgcctt cacctcggtc     3840

aaatcctaaa cactgcagcg cacatgaaat taaatcaacc cctccctccc tccctccctc     3900

ctctctccct ccatccctcc ctccctcctt ccctcgttca gagaccctgg caggctccgt     3960

ctcatacctg caggatgcgc atcccccggc ttggcgggcg aggtctgcgg aggacttgat     4020

gaacatggaa gtgcagatgg aggtagggac cgaggaaggg atagggaggg gggaggaagg     4080

gccgggccgg gaggggggga gggagtgaag gaggggagga cggaaggaaa gagggaggga     4140

gggagggagg gagggagaga gggagagagt aggaaatgag ggggataaga gatgagaata     4200

gcacggtttg ttcaatgtgc agcagagatg atcctctccg cacctcctcc actccctttc     4260

accccttcca tccctccctc cctcgctccc tcccttcctc cctccttcca ggcctggcgg     4320

gccctcctag ccgccaaggt ctccagagcc tcagagcggg tcttggcaag gcaggcggcg     4380

ttgcggggga acgaggcgca ggtagggagg gagggaggga gggagggcgg gagggaggga     4440

gggagggagg gaaggcgagg aggacggtga ggggaaaaga tctcctgaaa attgatgggg     4500

acagagtcgt tcaaggagtg tcaaaccaga atttcatgta tgcacacccg tccttccttc     4560

cctccatccc tccctccctc cctccctggc aggccttcaa cgagcatcag gtggagcttt     4620

tcgagtgcgc caagacccat gtctacttca atgtggcggc gcggtttgcc gaggcggtcg     4680

tggaggtcgg gtggaagaaa gggagggagg gagggaggga gggagggagg gagggaggga     4740

gggggaaaga ttgaaagcct accttgtcgt ctctttgcat acgcgcctat ttgtccagcg     4800

gttccaacgt tttcttttcc tccctccctc cctcatttcc tccctccctc cctccctcat     4860

ttcctccctc cctctctccc tccctccctc cctccctccc tccctccctc cctccctccc     4920

tccctccctc cctccaggcc ggcaccaccc accccgccct ggcccctgtc ctcgcccgcc     4980

tctgccacct cttctctctc tcgagccttc tagaagacga agcctccctg ctcgccagcg     5040

gtttcgcctc cgcggggcag atgcagctca ttcgcgaggt ggggaaggag ggagagaggg     5100

agagacggag ggagggacgg aaggagggag ggagggccat gtccatgtcg ctgctaaagg     5160

gcctgacgga caagaaatgg gaagatccaa tcgccgtgac tcatccctcc ctccctccgt     5220

cccgccctcc ctcccgccct ccctccaggc cgtgggcgcc ctcctcctcg ccctccgccc     5280

ggacgcggtg gcccttgtcg acgccttcaa ctattccgac gaagttttga actcacattt     5340

aggcaccgcc aacggcgata tttatacggg ctacctccaa caggtgcagc gcctcgtccc     5400

tgagaacaag gtccgcccgc tcgccctccc tccctccctc cctcccgccc tcccgccctc     5460

cctccctctt cttctcttcc ggggccttgt gcccgtgttg acgacgagca cttgacccgc     5520

ttctcgcttt cctcgatccc tccctcctac ttcccctctt tttccctccc cccttctcct     5580

ccctccttcc cttccaatta cgtcctcttc ccctccttca tccagctggc cgtcgccccc     5640

tacatcatga gggaggtgaa gcctttaatg caaggagcag acctgatctc cacggacgag     5700

gaggaggact ga                                                         5712


<210>  49
<211>  23
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  aco2-CRISPR target sequence including PAM sequence

<400>  49
ggcgaggacg acgcatacca tgg                                               23


<210>  50
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Entire chimeric guide RNA for targeting N. gaditana aco2 gene

<400>  50
ggcgaggacg acgcauacca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu                        103


<210>  51
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aco2 sense oligonucleotide

<400>  51
taatacgact cactataggc gaggacgacg cataccagtt ttagagctag aaatagcaag       60

ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt      120


<210>  52
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aco2 reverse complement oligonucleotide

<400>  52
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aactggtatg cgtcgtcctc gcctatagtg agtcgtatta      120


<210>  53
<211>  6956
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSGE6282

<400>  53
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt       60

aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag      120

ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct      180

ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt      240

tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg      300

cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct      360

gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc      420

gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg      480

tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa      540

ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg      600

gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg      660

ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga      720

tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt      780

ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct      840

gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga      900

acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agtttaaaca tatatgagtg      960

cgattcgatg caaggggggg aggggggaag gttcgaaagc cgtcgacctt ctggataaag     1020

tacggagttt gtcagccctc cctcgcggtg tggccacgat aagtaacaaa gcatgtaata     1080

aggatgcagc ctggagattg atgtgcgatc agaatcatca tgacacgtca aaacacaggc     1140

ggcgacatgg cgagacccgt gaccaacgtg atagagaaac ctgtcctgag gcacgtattg     1200

taaacaaccc agagatgtca gaaaaacaaa ggcatgtcaa cgctgtttaa ggcagcatct     1260

ccaggcaatc tttcctccgt gaggtggatc tatcctccct cctttctctt taaaagcggc     1320

cgtccgtccg tggtcgcctt ttccccactc tccctgcacg ttctgcccac acgtcccttc     1380

catcaccaca aaaagagcac attattctgt catgtacata cacagctggg acgggacgtt     1440

gcggagggcg ggccggggct tgtggcccgg gcaacaggac acctttccct gcccttaaac     1500

cccgactgtc tcctttttgc tcgtggagcc atgaccttcc tcctaactac cccacatccc     1560

gatacctgtc tatggtggaa agaagagtcc tcattgcgcg gtgtctgcac gagcaattac     1620

gaaagttaac ttctccagcc ccagccctag cgggcctctg tcgccctata tccctcatga     1680

gagccatggc agcctcttgc tgcgcccctt tccatccttt ttctacctca agtcatttta     1740

agatacattt tcaagggaac gtgggggatt gttgtgcgat cgaggcttct attctccgac     1800

acactttgaa tgagagcggt gcctctccta gtgagctcat ttgcaacagg agaaggccct     1860

cctcccttta caaatataaa gtgcccctgg tcgtctcctt gccactgtat accattgctg     1920

tgtctttgat tgaaaaaaat cgtaaccatg gctttggagg gccccatcgc cacgtttgcg     1980

cgagaacagc aatacttatc ccttttagtt ccttcctttc tcgcatttgt ggtagccccg     2040

tgcgcgcggg gaaatgagtt aaagtcatgt gcaccagaaa ctacacattc cattttttac     2100

cacagcataa aagccttatt ttctactgct acccagagcg gggccaccat ttctacggtg     2160

acacccgcca aaataaagga catatgcccg tgtgacgtat cgcggggcta tggaggggta     2220

cctgcgagtt gctgttgaag tgcccccccc cccccgtatg taaaaccacg tccttcggtc     2280

cgtacccatc tcaccgcaca ttgattacac tttacggcag aaatgacgac cgccaatgcc     2340

cgtttgtcga ggctcaaaga tcatttagca gagacggggg ctgtggcgcg cgcggtacgt     2400

gtggagtctt gctttcttca gggggggtcc cggggggggg gcgagtcaga caaagggtag     2460

agcgtactgg aaggacagac agatgctggc atgaccctgc atccagaaca cacatttaat     2520

aattgagggt ctggtgatcg aggggctcgg caaccgcgcg gctgctagca cgtgacacgg     2580

ttttttacct ctactgttcc acaatcaacc cctacttccc cttctccctc cctctttccc     2640

tccctcccct acgttaattt tccctcccgc tgtactatat cgcttcactt tcagccgatt     2700

agctcctctg ccatcagcgg ccgctccaca gcccgaaccc atgagagaga atcataatca     2760

aagatgagcc agccacgaag ctaccggaga attctgtaag aaaaatgttt aaagttgaaa     2820

atgctaacag tgaagtgata tcctttttta atggagtgtt gaggtgaagt ctagcatcgt     2880

aggggaaaac aggattctgt gtcttccatt ctactccttg ataaagcgaa gaaatccgac     2940

aaaaccaaag agattgttca agtttaagat ttgtaagcgt acaactatga acttcttctc     3000

tttgtaggcc tgagtggtcg tatgcatacg attcatgaag tgaatcagta tcgctggatt     3060

ttgcttagga gtaaagcaca actaagaaaa tatgctgcct ggcaggcatc ctgagacatg     3120

aggcaagcga cgtagcaatt gaatcctaat ttaagccagg gcatctgtat gactctgtta     3180

gttaattgat gaaccaatga gctttaaaaa aaaatcgttg cgcgtaatgt agttttaatt     3240

ctccgccttg aggtgcgggg ccatttcgga caaggttctt tggacggaga tggcagcatg     3300

tgtcccttct ccaaattggt ccgtgtggta gttgagatgc tgccttaaaa ttctgctcgg     3360

tcatcctgcc ttcgcattca ctcctttcga gctgtcgggt tcctcacgag gcctccggga     3420

gcggattgcg cagaaaggcg acccggagac acagagacca tacaccgact aaattgcact     3480

ggacgatacg gcatggcgac gacgatggcc aagcattgct acgtgattat tcgccttgtc     3540

attcagggag aaatgatgac atgtgtggga cggtctttac atgggaagag ggcatgaaaa     3600

taacatggcc tggcgggatg gagcgtcaca cctgtgtatg cgttcgatcc acaagcaact     3660

caccatttgc gtcggggcct gtctccaatc tgctttaggc tacttttctc taatttagcc     3720

tattctatac agacagagac acacagggat catggggaag aaaccggaac tgaccgctac     3780

gtccgtggag aaattcctta ttgagaagtt cgactctgtc tccgacttga tgcaactgag     3840

cgagggagag gagagtaggg cgttctcgtt tgacgtaggg ggtcggggat acgtgttgag     3900

ggttaatagt tgtgcggacg ggttctacaa ggatcggtat gtctaccgtc atttcgcctc     3960

cgccgctctc cccataccag aggtactgga cattggggag tttagcgaat ctctcacgta     4020

ctgcatctcg cgccgagccc agggagtgac gttgcaagat ctgcccgaaa ctgaattgcc     4080

tgccgttttg caacccgtgg ccgaggccat ggacgcgatc gctgccgcag atctgtctca     4140

gacgtccggc tttggacctt ttgggcccca gggcatcggg cagtacacga cctggcgaga     4200

cttcatctgc gccattgccg atcctcacgt ctatcattgg cagacagtca tggatgacac     4260

cgtgtctgca tccgtggccc aagcactgga cgaactcatg ttgtgggccg aggattgccc     4320

tgaggtcagg cacctggtgc acgcggattt cggcagcaat aacgtactta cagacaatgg     4380

tcggattact gctgtcatcg actggtccga agcgatgttt ggtgatagcc aatacgaagt     4440

ggcgaacata ttcttctggc gtccctggtt ggcgtgcatg gagcagcaga cacgctactt     4500

tgaacggagg cacccggagc tggccggctc cccacgactc cgcgcctata tgttgcgtat     4560

cggactcgat cagctttacc agtctctcgt cgacggcaac ttcgacgacg ccgcgtgggc     4620

gcagggccgc tgcgacgcga tagtccgcag cggggctggg acggtgggtc ggacccaaat     4680

cgcacgccgg tcggctgcgg tgtggacaga cggctgtgtt gaggtgcttg cggactcggg     4740

caaccgtagg ccgagcaccc gaccgcgtgc aaaggagtga ttgaatcatt gaatgaacca     4800

ttgtgtgcag aatcgatttc gggagtgttg ccaacacaag aaatatgccc agggttgtgt     4860

agaagtttgc gtgaatgtga tgaagggaag ccatacgctg aattatcgtg acgtgtgtga     4920

gacgaagtgt cacatcatac acccaatttg agaagctgta cctattagaa gaatttgtga     4980

gatacattaa accccttttg gtacgtggta taattgttat ttgggaagct gtaaacacgc     5040

agatcgttcc tgagattgtc aattactttt gtggtgtttc ctaaaggccg catcactgcc     5100

cgaatcgagt tgatggcccg caaaggcgcg ccgagcatga ccgaggtcaa ggtgggtgaa     5160

ataaaaacat aaaaagagaa taaacaacga aaggcgaggt ctttggggga tgcctgaata     5220

cgtaggcaac cgtactgatc gttttggacc cttcttctct tggggccgat ggcccacagg     5280

agggcatgat ggcggacttg gcggcgaatc cggtctttac ggacccagaa tggaacgact     5340

tgaacagaga tcaggtaggg atatgggtga ttggccggaa ggtggggaag gagggggagg     5400

tgccccttgt gagcttgccc tgtggtcgga gcgcttccct cgatcaaccc ctccctctcc     5460

cttccccttc ccccctctgg ctagatccgt gaacgcacca tctctcgact gagagctgcg     5520

tacaagctcc tgatccgaga cggtgccgat gtcagccgcc ggaatgcccg gcttgagatt     5580

cacgccctcc atgacttggg tagcccttcc tccctccctc cctccctccc tcccttgctc     5640

cctacctcct ccccgtgtcc tcaccttcgt tttttccaag catttattac ttttcagttt     5700

catgtggacg atcatcaaaa tgatgccttc ctcgcccgac gcgattggtc ctccctccct     5760

ccctctttct ctccctccct ccctccctcc ctccctttgc agggtggtac gtgcggcagg     5820

gtgtgcattt cggcctcttt atgggcgcct tggccgggca ggggagcgac gaacaacgcg     5880

ctgagtggtt taaacgacga aagggcctcg tgatacgcct atttttatag gttaatgtca     5940

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     6000

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     6060

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     6120

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     6180

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     6240

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     6300

cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac     6360

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     6420

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     6480

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     6540

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     6600

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     6660

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     6720

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     6780

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     6840

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     6900

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaa         6956


<210>  54
<211>  1767
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  aco2 target upstream homology arm

<400>  54
atatatgagt gcgattcgat gcaagggggg gaggggggaa ggttcgaaag ccgtcgacct       60

tctggataaa gtacggagtt tgtcagccct ccctcgcggt gtggccacga taagtaacaa      120

agcatgtaat aaggatgcag cctggagatt gatgtgcgat cagaatcatc atgacacgtc      180

aaaacacagg cggcgacatg gcgagacccg tgaccaacgt gatagagaaa cctgtcctga      240

ggcacgtatt gtaaacaacc cagagatgtc agaaaaacaa aggcatgtca acgctgttta      300

aggcagcatc tccaggcaat ctttcctccg tgaggtggat ctatcctccc tcctttctct      360

ttaaaagcgg ccgtccgtcc gtggtcgcct tttccccact ctccctgcac gttctgccca      420

cacgtccctt ccatcaccac aaaaagagca cattattctg tcatgtacat acacagctgg      480

gacgggacgt tgcggagggc gggccggggc ttgtggcccg ggcaacagga cacctttccc      540

tgcccttaaa ccccgactgt ctcctttttg ctcgtggagc catgaccttc ctcctaacta      600

ccccacatcc cgatacctgt ctatggtgga aagaagagtc ctcattgcgc ggtgtctgca      660

cgagcaatta cgaaagttaa cttctccagc cccagcccta gcgggcctct gtcgccctat      720

atccctcatg agagccatgg cagcctcttg ctgcgcccct ttccatcctt tttctacctc      780

aagtcatttt aagatacatt ttcaagggaa cgtgggggat tgttgtgcga tcgaggcttc      840

tattctccga cacactttga atgagagcgg tgcctctcct agtgagctca tttgcaacag      900

gagaaggccc tcctcccttt acaaatataa agtgcccctg gtcgtctcct tgccactgta      960

taccattgct gtgtctttga ttgaaaaaaa tcgtaaccat ggctttggag ggccccatcg     1020

ccacgtttgc gcgagaacag caatacttat cccttttagt tccttccttt ctcgcatttg     1080

tggtagcccc gtgcgcgcgg ggaaatgagt taaagtcatg tgcaccagaa actacacatt     1140

ccatttttta ccacagcata aaagccttat tttctactgc tacccagagc ggggccacca     1200

tttctacggt gacacccgcc aaaataaagg acatatgccc gtgtgacgta tcgcggggct     1260

atggaggggt acctgcgagt tgctgttgaa gtgccccccc ccccccgtat gtaaaaccac     1320

gtccttcggt ccgtacccat ctcaccgcac attgattaca ctttacggca gaaatgacga     1380

ccgccaatgc ccgtttgtcg aggctcaaag atcatttagc agagacgggg gctgtggcgc     1440

gcgcggtacg tgtggagtct tgctttcttc agggggggtc ccgggggggg ggcgagtcag     1500

acaaagggta gagcgtactg gaaggacaga cagatgctgg catgaccctg catccagaac     1560

acacatttaa taattgaggg tctggtgatc gaggggctcg gcaaccgcgc ggctgctagc     1620

acgtgacacg gttttttacc tctactgttc cacaatcaac ccctacttcc ccttctccct     1680

ccctctttcc ctccctcccc tacgttaatt ttccctcccg ctgtactata tcgcttcact     1740

ttcagccgat tagctcctct gccatca                                         1767


<210>  55
<211>  755
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  aco2 target downstream homology arm

<400>  55
gagcatgacc gaggtcaagg tgggtgaaat aaaaacataa aaagagaata aacaacgaaa       60

ggcgaggtct ttgggggatg cctgaatacg taggcaaccg tactgatcgt tttggaccct      120

tcttctcttg gggccgatgg cccacaggag ggcatgatgg cggacttggc ggcgaatccg      180

gtctttacgg acccagaatg gaacgacttg aacagagatc aggtagggat atgggtgatt      240

ggccggaagg tggggaagga gggggaggtg ccccttgtga gcttgccctg tggtcggagc      300

gcttccctcg atcaacccct ccctctccct tccccttccc ccctctggct agatccgtga      360

acgcaccatc tctcgactga gagctgcgta caagctcctg atccgagacg gtgccgatgt      420

cagccgccgg aatgcccggc ttgagattca cgccctccat gacttgggta gcccttcctc      480

cctccctccc tccctccctc ccttgctccc tacctcctcc ccgtgtcctc accttcgttt      540

tttccaagca tttattactt ttcagtttca tgtggacgat catcaaaatg atgccttcct      600

cgcccgacgc gattggtcct ccctccctcc ctctttctct ccctccctcc ctccctccct      660

ccctttgcag ggtggtacgt gcggcagggt gtgcatttcg gcctctttat gggcgccttg      720

gccgggcagg ggagcgacga acaacgcgct gagtg                                 755


<210>  56
<211>  5607
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 with FLAG tag, nuclear localization sequences, peptide 
       linker, and introns, codon optimized for expression in 
       Parachlorella

<400>  56
atgcccaaga agaagcggaa agtcggggac tacaaggacg acgatgacaa actggagcct       60

ggggagaagc cctataagtg tcctgagtgc gggaagagct tcagccaatc tggagcactg      120

acaaggcacc agaggacaca tacacgcgac aagaagtaca gcatcgggct ggatatcggg      180

accaattctg tgggatgggc cgtgattacc gacgagtata aggtgcccag caagaagttc      240

aaggtgctgg ggaacacaga ccgccacagc attaagaaga acctgatcgg ggcgctgctg      300

tttgattctg gagagacagc agaggcaacc gtgagtgaga acagttttca gatcgaatag      360

cacccccccg cctctgcagc agtcgcatac cggctgcagt aatagcttgg ttcaacggcg      420

acctgaacaa gtactgtagt ttctatgcat acgaacttta tcgaatagaa tcacgcttgg      480

gtatcgatca taccttagcg ctcaatttca ttggctgcta cagaccatat tttcctcttc      540

acttgttgca gcgcctgaaa agaacagcaa gaaggcgcta cacccgccgc aagaatagga      600

tttgctacct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc      660

atagactgga ggagtcgttc ctggtggagg aggataagaa gcacgagagg caccccatct      720

tcggtgagaa gagtttggct accaaatcta tcttttcata tcacatatac cgcctgatat      780

tctgaggtgg tggcttttgt ctttttcttt cagtattttt cttcgttggg aacctaccgc      840

gagggcattc attgtggcgg atctgtaagt gcgaccaggc tgtatccaat attttttcct      900

atcgcaggga acattgtgga tgaggtggcc taccacgaga agtaccccac aatctaccac      960

ctgcgcaaga agctggtgag aatctctgct tgtcgaatgt gtccagttgt gtcttgaatc     1020

ctggcaagat gttcttttca ccatccgtcc tgcaaaagtg tcagaagtag catctctcga     1080

tcgcgttgtc acttcaacgc ctccgcaact ccccccgttg tgaatcctgt ggtcatggct     1140

cagcttttca gatctctacc tgcatgttgt ttgcctgtct cagtcctgcc tgcacaaatc     1200

atcgcccttg tttactcctt gcaatcacgg attgtgtgca ggtggacagc acagataagg     1260

ccgatctgag gctgatctac ctggcattgg cccacatgat caagtttagg gggcacttcc     1320

tcatcgaggg ggatttgaac cccgacaaca gcgatgtgga caagctgttc atccagctgg     1380

tgagtggagg gctggggttt gggggtgggg ggtggggagg gaggcacgga tggtgttttc     1440

tcatgtccaa ccgtggttca tgcaaccgaa cagcagtttc acaagatggt tccaacaggg     1500

tgctccattt ctccctgaca aaacctcgtg cggtccatct ggtatagctg ggttagtagg     1560

gggttgtggg ctgtccacag tcagtgcgaa gcaggctcta ttgagcgtgt gctagtgtgt     1620

gctgtgctga ttggcatttt gttgggccga gtgttaggat tagggtaaat caccctaatt     1680

aaccttacat aataggactg tatgcaaatt tgttttccaa aaactctacc cagcgtggtc     1740

agactgcatg cactgtggag catgcatggg gctgaccctg ttgatcctgc tcattctgct     1800

tcctccaggt gcagacctac aaccagctgt ttgaggagaa ccccatcaac gcatctgggg     1860

ttgacgcaaa ggccattctg tctgcaaggt aggtgcagga agaagtgaat gatgcacaca     1920

tggtggaatc gtgatacaag cagcagcaag tgttggacca agacatgtgc gtgctttgct     1980

gctgccaagc tggcactgca ccaggtcgtg cattgatctg cacatttgat atactgtgag     2040

agtcagacga cgtcctttca gagcctgtgt gtgattctcc aggggttaac acgagtttcc     2100

tttctgccag tgagtcaccc tctcgctgct cgctcctggt gcaggctgag caagtcaagg     2160

agactggaga acctgatcgc ccaattgcct ggagagaaga agaacgggct gttcgggaac     2220

ctgatcgcat tgtctctggg gttgaccccc aacttcaaga gcaacttcga cctggcagag     2280

gacgcaaaac tgcagctgag caaggacacc tacgacgatg atctggacaa cctgctggcc     2340

cagattggag atcagtacgc agacctgttc ctggcagcca agaatctgag cgacgcaatt     2400

ctgctgagcg acattctgcg cgtgaacacc gagatcacca aggcacctct gagcgcaagc     2460

atgatcaaga ggtacgacga gcaccaccaa gacctgacac tgctgaaagc actggtgaga     2520

cagcagctgc ctgagaagta caaggagatc ttcttcgacc agagcaagaa cgggtacgct     2580

gggtacattg atggaggagc aagccaagag gagttctaca agttcatcaa gcccatcctg     2640

gagaagatgg acgggacaga agagttgctg gtgaagctga atcgcgagga tctgctgagg     2700

aagcagagga cattcgacaa tgggagcatc ccacaccaga tccatctggg agagctgcac     2760

gcaattctga ggagacaaga ggacttctac ccgttcctga aggacaatcg cgagaagatc     2820

gagaagatcc tcacgttccg catcccgtac tatgtgggac ctctggcaag ggggaactct     2880

agatttgcct ggatgacccg caagagcgag gagacaatta caccctggaa cttcgaggag     2940

gtggtggata aaggggcatc tgcacagagc ttcatcgaga ggatgaccaa cttcgacaag     3000

aacctgccca acgagaaggt actgcctaag cattcactgc tgtacgagta cttcaccgtg     3060

tacaacgagc tgaccaaggt gaagtacgtg acagagggga tgaggaagcc agcatttctg     3120

agcggagagc aaaagaaggc catcgtggat ctgctgttca agaccaaccg caaggtgacc     3180

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgacag cgtggagatt     3240

tctggagtgg aggaccgctt caacgcatct ttggggacat accacgacct gctgaagatc     3300

atcaaggaca aggacttcct ggacaacgag gagaacgagg acatcctgga ggacattgtg     3360

ctgacactga ccctgttcga ggatagggag atgatcgagg agcgcctgaa gacatacgca     3420

cacctgtttg acgacaaggt gatgaagcag ctgaagagga ggcgctatac tggatgggga     3480

aggctgtcaa ggaagctgat taacgggatc cgcgacaagc agagcgggaa gacaattctg     3540

gacttcctga agagcgacgg gttcgcaaac cgcaacttca tgcagctgat ccacgacgat     3600

agcctgacct tcaaggagga catccagaag gcccaagtgt ctggacaagg ggatagcctg     3660

catgagcaca tcgcaaatct ggctgggtca cccgcaatca agaagggaat tctgcagacc     3720

gtgaaggtgg tggatgagct ggtgaaggtg atgggaaggc acaaacccga gaacatcgtg     3780

atcgagatgg caagggagaa ccagacaacc cagaagggac agaagaactc tagggagcgc     3840

atgaagcgca tcgaggaggg aattaaggag ctgggaagcc agatcctgaa ggagcatcct     3900

gtggagaaca cccaactgca gaacgagaag ctgtacctgt actacctgca gaacgggagg     3960

gacatgtacg tggatcaaga gctggacatc aaccgcctga gcgactatga cgtggaccac     4020

attgtgcctc agtcgttcct gaaggacgac agcatcgaca acaaggtgct gacaaggagc     4080

gacaagaatc gcggaaagag cgacaacgtg ccttcagaag aggtggtgaa gaagatgaag     4140

aactactggc gccagctgct gaacgcaaag ctgattacac agcgcaagtt cgacaacctg     4200

accaaggcag agaggggagg actgtcagaa ctggataagg ccgggttcat caagaggcaa     4260

ctggtggaga cacgccagat cacaaagcat gtggcccaga ttctggacag ccgcatgaac     4320

accaagtacg acgagaacga caagctgatc cgcgaggtga aggtgattac cctgaagagc     4380

aagctggtga gcgactttcg caaggacttc cagttctaca aggtgcgcga gatcaacaac     4440

taccaccacg cacacgacgc ctacctgaat gcagttgtgg gaacagccct gatcaagaag     4500

taccccaagc tggagagcga gttcgtgtat ggggactaca aggtgtacga cgtgcgcaag     4560

atgatcgcca agtctgagca agagatcggg aaggcaaccg ccaagtactt cttctacagc     4620

aacatcatga acttcttcaa gaccgagatc accctggcca atggggagat taggaagaga     4680

cccctgatcg agaccaacgg agagactgga gagatcgtgt gggataaggg gagggacttt     4740

gcaacagtgc gcaaagtgct gagcatgcct caagtgaaca tcgtgaagaa gaccgaggtg     4800

cagactgggg gattctcaaa ggagagcatt ctgcccaagc gcaacagcga taagctgatt     4860

gcacgcaaga aggactggga ccccaagaag tatggggggt ttgatagccc caccgtggca     4920

tattctgtgt tggttgtggc caaggtggag aaggggaaga gcaagaagct gaagagcgtg     4980

aaggagctgc tggggatcac cattatggag aggagcagct tcgagaagaa ccccatcgac     5040

ttcctggagg caaaggggta taaggaggtg aagaaggacc tgatcatcaa gctgcccaag     5100

tacagcctgt tcgagctgga gaatgggagg aagaggatgc tggcatctgc tggagaactg     5160

cagaagggga atgagttggc actgcctagc aagtacgtga acttcctgta cctggccagc     5220

cactacgaga agctgaaggg atcacccgag gacaatgagc agaagcagct gtttgtggag     5280

cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttcag caagcgcgtg     5340

attctggcag acgcaaacct ggataaggtg ctgagcgcct acaacaagca ccgcgataag     5400

cccattcgcg agcaagcaga gaacatcatc cacctgttca ccctgaccaa cctgggagca     5460

cctgcagcat tcaagtactt cgacaccacc atcgaccgca agaggtacac aagcaccaag     5520

gaagtgctgg acgcaaccct gattcaccag agcattactg ggctgtacga gacacgcatc     5580

gacctgtcac aactgggagg ggactga                                         5607


<210>  57
<211>  588
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  RPS17 promoter

<400>  57
caacacctag ttggtaaata ccgttgctga tattgctctg taccagtaaa agagggctgc       60

gatgagcgtt tttagtgcac ttcttcaaca cggaatattt ttcacaaatt ggtatgagaa      120

ccaattttgc aaaatgttcg ccctgtaaag tatcgctctg ggacgatcag cttgacgtaa      180

ttgtaggcga aaagggcgtt caaagtgcag ctttatgtat gaacgtcata aaatataaag      240

catagcacaa tcactgatag aaaatatttg tgcgcattaa aactctcact tctgttgcgg      300

atacaacgac ggaaatgaga agcttgtgta agaagcaatt caagttttca ttttgtcatc      360

taaggtgtga tcctccgata ttcattaccg aatgctgatc tgagttggaa agatggcaat      420

atttagctgt gcacactttg acctccaggc cttggcggga atttagtatt ctagctttcc      480

tattggaacg ataggccagc caagtctcca gcttgtatac gctacaccag cagacatgct      540

ctcaatttag ctgacagtgt cttcatattt gtattatctg ttgtgtct                   588


<210>  58
<211>  455
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  RPS17 terminator

<400>  58
ggtgcgaata gtgcttcagt aaaaaagtag caacttggtg caatatcgtc agggtcgtgt       60

ggtctgctcg ccagcaagtt ttttggcaca ggagagcgct ttttccgagt accgccaaag      120

ttcaagcatg tgctgtgatt cgctgttgcc tcttatgata attgctcaaa gtttccaagc      180

attctatgtc caccctgcac cactaagttg tatggtgctt attctgcagg ggatgattca      240

tggtgcctaa aaattttgtg ctgctgtcgc gtctgttttc tgtcgcagtt tagtgaatgt      300

aactccaaat accaaacttt tcatcacaat catattgatg cctttgtaag tgaattacag      360

cgttttttgc cataaaaaga agtaccgtga cattggggtc gtcataacaa gaagctttat      420

gaacaagcag cttgatctac gagacttata cataa                                 455


<210>  59
<211>  2691
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BlastR gene codon optimized for Parachlorella and containing 
       introns

<400>  59
atggcaaaac ctctctccca ggaagagtct accctgattg aaagggcaac agcaaccatc       60

aacagcattc ccattagcga ggactactct gttgtgagtt ctgagaagct gattgttgtt      120

taacttcttt gaaagcttta tcgaagattc tgcaagcgat gaacattgct tgtcaagacc      180

gagagctgca tgcccacttg acatccagct ttgaacggct cttcatgttt gatttgtttc      240

tgattgtagg catctgcagc attgagctct gatggaagga tctttacagg agtgagtgca      300

gcgtcagctg tggcagttgt tggctttcgt ctcagtcagt agtttgctgg gattgattat      360

ggagggcaca gttgcaattt tgagttgcac gttgcgacaa gcgtgttgac aaagcgtggt      420

caagccggcc agtcttgccg gtggcgggtg gcttggtcta acttccgctc tacggcaatc      480

gttttgttca tggttacggg gctggcgtgc cagaaagtcc tggtcagcca ccctcgcttc      540

aaagccgtag cccaacaact ttgcgaatat gttcgatttg caggtgaacg tgtaccactt      600

tacaggagga ccttgtgcag aattggtggt gttaggtaca gctctgcgtg caacaggttg      660

caagatgcag cgcaggtctt ccctggtcaa acgatgtatg cagagttgag aggcacttga      720

gctgggtgaa tggcgtgggc tcgtaggtag tgtgcagggc aggaagggca gccaattttg      780

gagttgtggt ccggtgtcgt tgcttcgagc cttattagga ctcttgctca tcaaagcgtt      840

agttgtgaat aagttgatct gaaaggatgt tatgtacagc aagcagcagc agttaagagt      900

ctggggagta gctgcacagg gcgaggtgtc aagatgggaa gggtcctgcc tccttatgtg      960

tttttccctg taggggagga agcctcttat gggcaatggt tgggcatatt ttccagccag     1020

cccttctttc tataggggcc agggtgggcc cagctcgtct tggcttccac caccaggaga     1080

gtgagggcat tgaagggcca taaatagtcc tcccatctac gtgcaccaga gggtgtcgtc     1140

taggctgtgc atgccacgag gggaaggagc caagaatgag tgtatgggtt gttttcatgt     1200

ttaggctggg ataaaactgt tttcaattgc gcctgccggg tgaaaaccac agcagcatca     1260

gcaagcttgg agaaggccag cccgcccagc acaggctcac gttcccactc aggcggtcag     1320

tcgggcgggg gtgtgagtca ggcaggcgag ggtgtctgtg cctgacatca gcacctctgc     1380

ttagccactg cagcccctgg agcagggtag ggcgtcattt gcagcaatca cctgctgcct     1440

cacacgtcgc agcttggaat ttcaacgacc atcagcgctg gggttgttga gggatcatag     1500

cagattttgg tgcagcctgg ttgtcatgct ctttgtggaa tggcctctat gttcgagcaa     1560

ttcgttggat gttgaggtgc ttggggacag agagtcgaat gatgggccag ggtcaaacat     1620

gcgagcgttt ggctgagtca gcggtttttg ctggtcactt tttcttttgt ttcttattta     1680

ggtttgatgg atgtgttttg tgctgctgcc ctgaagctgc agcagcgtgt ctgccctgcg     1740

ctactgcggg caccaaggct atgtgctggt gcactcggct gcgctgcacc tgtgcacctc     1800

gcactccgtc cagcctccat gcagcacacg tactcacggt gtcctcctga cctgtcgtac     1860

gctattccaa acttgctctt ttgctgccgc tgctctcgta cacaattgct gttgattatc     1920

gatatctaat cgagcgcctg ctgactgaac tccgcaggta cagcagctgc agcagcagca     1980

ggaaatttga catgcattgt ggcaataggt gggtgggctc tgaaggagga ggagggagcg     2040

ggtgattaaa cagggcctgc atgaagagga gcaggggctg cgtggacagc agggggaagg     2100

tgcagaaggg agggtcaagc ggggttcagg tggctgtggg tttctgcacg agcagtgaaa     2160

gaagctgtat ccttccacct gcttccactg gcgaaaggtt gaaaacagga tgtcgcagct     2220

ggaaagatgt tgcgctgtca agtgcaagcc atggttgagg gtatgcctgt gtgcatgtgc     2280

ttcttaaagt tactcctgtt ctatggttct gggtgcttgt tgtttgtggt gcagggaacg     2340

agaatagggg gattttgtca ccttgcggaa gatgtagaca ggtgttgttg gatctgcatc     2400

ccgggattaa ggtgaggggg catgtaagca atggcaggca attcaagaac gaatcattgc     2460

tgcaaatgct gggatggtat gcagctgagg tatctattgc cttgtatttt gtctcgcatt     2520

gcatcggtgg tgcgttctgt ggcctgaggc acagttcttg ctgtttgata agggttcgac     2580

tgagttgtcg tgtgtgctgt gctgcaggca atcgtgaagg attcagatgg gcagcctaca     2640

gcagtgggaa ttagagaact gctgccttct gggtatgtgt gggaaggata a              2691


<210>  60
<211>  531
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  RPS4 promoter

<400>  60
ccaccatggg ggaggtttga agtgtgcgcc tgatataatc atacacctaa aagcaccact       60

tgctgattgt gaagggacta tgtcgtttat gacgggacgt tacgctggcc gatggtttga      120

atttggacgc tgtggtagaa tgttatatgg acgtaaaggt tggcatattg aaaatcgtct      180

tcacaggcaa acttctagac gtgtgaccca ccggtaaaac gacaagcgtg gcgcgtcgat      240

tgcgctttga acgtcgtttg ttggactcca gatgaacctc aaaatcaaag cggtgattga      300

cgaaaatcaa atgacagccc gcaaaatttc atcagccttc ggatcggatt ctcagaatct      360

gattgtccct gctggctaca tttatgaaat ttcgtacatt ttggcagaaa tgtcccaata      420

ccatagcact gccgcctgag ctcacccgag caatgcatac tgggtacctc gcccatctcg      480

ccctctttcc aagcccagtg ctgttgtaaa tagccaaagg gctcagtaac a               531


<210>  61
<211>  546
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  RPS4 terminator

<400>  61
gcatagcatc agcctgtggc agggttgtgg tagggctgag tggcagggtt aaaggggttg       60

cctaccccac ccctactctc atgacaccag caacagcagc agctcatgca gtactcaaat      120

cactgatgtc aatggtgtga cacatttggt taaggctgct ttttaaagtg ctgctttggg      180

ggcagtgact gtgcagagct tggagcgtat ccccatgtaa tcagaaccga cgagagttcg      240

gggcaacctt tcatcttcac attttttgtg atcagctaca gagtctgaaa tcaaatagag      300

gctgccatct aaacgcagga gtcacaacga aggcgaaaac tccaattgct gtactcaatg      360

cactaagtga ttgttcaatg gataaataca ctatgctcaa ttcatgccag cagagctgct      420

ccttccagcc agctacaatg gctttttcca cgccttttga agtatgaatg ttcagcttgc      480

tgtgcttgat gcatcaccat aaacacaatt ctacaacatt tcatgccaac aacagtacgg      540

gctttc                                                                 546


<210>  62
<211>  572
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  ACP1 promoter

<400>  62
agtttgcata gttaagtatg ctggctattg cagtacctta tatgcaaaca agtgctcaat       60

ctgtttcatc attgtctgtg ggcaaattgc ctgccaatat tctccagtta ttgcctgttg      120

tttcaaatga ttgaaattgg aagttgtatt gctctacatt tttgacttgt gattttttca      180

tttgttgata tctgacaact gtgaactgca ctgaacttgc tgtgcttata aatgcatttt      240

tttgttttgg gccacgttga ttccttgtga tactttcctg ctatcaaacc aaaaatatac      300

tctcatgact gacgtgcaac aaatgcatgg aagctttcaa cgttacgaca gctgcttgcc      360

ccccatcagc tattctacat gtgtaaccta ccttgcatgg ccaccacaac gctactgcat      420

gcaagatctg gcgcaactgg atgtcccaat agtagaagta tccggattat ctccgagagt      480

tttacatatg taatcgacgc catttctgtc atcaactata aatccattgc tcctgcattt      540

ctggcactga cattctacca caagcaatac ca                                    572


<210>  63
<211>  869
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  ACP1 terminator

<400>  63
gcagcagctt gttatgcctt ccccatgggc atcagcatgc tgcaagctgt ctagatatcc       60

agctttcagt ggaggttgag cgagggtcag cagcggttcc ctggcgatgg cggtcagctt      120

ttctggaagc cttcactagg actgcgccca gcgcatgtga cgccaatcga acttgtgtgc      180

aaggccaaat tttgtgaccc tgtgctgcac ttcatgtatt caagaattga gaagaaattt      240

cattgctgcc cttctttcac tttaatttcc atccctggat ccacctccca ccattgtggt      300

tgatgggtag gggttttggg taggtgcagt tcgttgtgca cgttgacatg tgtaacggtg      360

agcaaaggaa ttgctgggca agtagctatt gcagcttaag ggcatggtga aacacttgtg      420

ctgtatttac agaggaagcc agacaggtaa ggagtgtgtg gcagcttgga acaggagggc      480

tggtcgcaac aagtatgcat atcccatgat tgttgacata agagcagcag gtgcatattg      540

ccagcctttg tgaaagtgga ttgaaaatcg attagttggt gtgatagctg aggctaggca      600

ctgccaacct gcagtgaaat gaggctccaa gaccgggtaa taatacaggc aatcgaatcc      660

agttgaaatt acggcgatta aatccaagcg agcgttgtaa gaacatctgc acctgtctga      720

agtagtgagc ggataatgag cattgcttgc cttctatcac tatacctgac agttacgtgt      780

cacacactct caagcacaac acacagcggc aaagttactt gctaaacctc acagtcaagc      840

tgaaaataaa ggctaaatta cgtgagacc                                        869


<210>  64
<211>  23
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  SRP54 CRISPR target sequence (including PAM)

<400>  64
ggcgtgggac atggtgcgca agg                                               23


<210>  65
<211>  1707
<212>  DNA
<213>  Parachlorella sp.


<220>
<221>  misc_feature
<223>  encodes cpSRP54

<400>  65
atgcttcggc agcagctgtt gcacagcggc aggcagccgg gtgcgacatg cagcttacta       60

acctgctcga catggcgacc gtctgccttg ttcggccgtc ctaagcccca aaaactgcac      120

agccagcgct tgcagcatca gggccgcccc tcccgcctcg tcgtgcgcag cgcaatgttc      180

gacaacctga gccgcagcct ggagagggcg tgggacatgg tgcgcaagga cgggcggcta      240

acggcggaca acatcaagga gcccatgcgg gagattcgca gggcgctgct tgaggcggat      300

gtgaggctgg gggcgccgct gatcagattc ttggtatcta cccccccccc ctcccaggtc      360

tccctccccg tggtgcgcaa gtttgtgaag gcggtggagg agaaggcgct gggttctgca      420

gtgaccaagg gtgtcacccc cgaccagcag ctggtgaagg tggtgtacga ccagctgcgg      480

gagctgatgg gggggcagca ggaagggctg gtgcccactt cgccagagga gccgcaggtg      540

atcttgatgg cggggctgca gggcacgggg aagacgacag ctgcggggaa gctggccttg      600

ttcctgcaga agaaggggca gaaggtgctg ctggtggcca ccgacatcta ccgccccgcc      660

gccatcgacc agctggtgaa gctgggcgac aggatagggg tgccggtgtt ccagctggga      720

acccaggtgc agccgccgga gattgcaagg caggggctgg agaaggcgcg agcagagggg      780

tttgacgccg tcatcgtcga cacggcgggg cggctgcaga tcgaccagag catgatggag      840

gagctggtgc agatcaagtc cacggtgaag ccctccgaca cgctgctagt ggtcgatgcg      900

atgacggggc aggaggcagc cgggctggtg aaggcgttca atgatgccgt ggacatcaca      960

ggcgccgtgc tgaccaagct tgacggggac agccgcggcg gcgccgcgct gagcgtgcgc     1020

caggtcagcg ggcggcccat caagtttgtg ggcatggggg agggcatgga ggcgctggag     1080

cccttctacc ccgagcgcat ggccagcagg attctgggca tgggtgacgt ggtcaccctg     1140

gtggagaagg ctgaggagag catcaaggaa gaggaggcgc aggagatatc gcggaagatg     1200

ctgtcggcca aatttgactt tgacgacttc ctgaagcagt acaagatggt ggcggggatg     1260

gggaacatgg cccaaatcat gaagatgctg ccaggcatga acaagtttac ggagaagcag     1320

ctggcgggcg ttgagaagca gtacaaggtg tacgagagca tgatccagag catgacggtg     1380

aaggagcgca agcagccgga gctgttggtg aagtcgccct ccaggaggcg gcgcatagcg     1440

cgcgggtcgg ggcgctcgga gcgggaggtc acagagctgc tgggggtgtt caccaacctg     1500

cggacgcaga tgcagagctt ctccaaaatg atggccatgg gggggatggg catgggctcc     1560

atgatgagcg acgaggagat gatgcaggcc acgctggcag gcgccggccc ccgccccgtg     1620

ccagctggca aggtgcggcg gaagaagctg gccgcggcgg gcgggtcgcg gggcatggct     1680

gagctggcat ccctgaaggc agaatga                                         1707


<210>  66
<211>  2667
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bleomycin resistance gene, codon optimized for Parachlorella and 
       containing introns

<400>  66
atggccaaac tgacatccgc tgttcctgtg ttgacagcaa gagatgttgc aggtgcagtg       60

gagttttgtg agttctgaga agctgattgt tgtttaactt ctttgaaagc tttatcgaag      120

attctgcaag cgatgaacat tgcttgtcaa gaccgagagc tgcatgccca cttgacatcc      180

agctttgaac ggctcttcat gtttgatttg tttctgattg tagggacaga tagactgggg      240

tttagcaggg actttgtgga ggacgatttt gcaggagtgg tgagggatga tgtgacactg      300

tttatctcag cagtgcagga tcaagtgagt gcagcgtcag ctgtggcagt tgttggcttt      360

cgtctcagtc agtagtttgc tgggattgat tatggagggc acagttgcaa ttttgagttg      420

cacgttgcga caagcgtgtt gacaaagcgt ggtcaagccg gccagtcttg ccggtggcgg      480

gtggcttggt ctaacttccg ctctacagca atcgttttgt tcatggttac ggggctggcg      540

tgccagaaag tcctggtcag ccaccctcgc ttcaaagccg tagcccaaca actttgcgaa      600

tatgttcgat ttgcaggtgg tgcccgataa tacactggca tgggtttggg tgagaggtac      660

agctctgcgt gcaacaggtt gcaagatgca gcgcaggtct tccctggtca aacgatgtat      720

gcagagttga gaggcacttg agctgggtga atggcgtggg ctcgtaggta gtgtgcaggg      780

caggaagggc agccaatttt ggagttgtgg tccggtgtcg ttgcttcgag ccttattagg      840

actcttgctc atcaaagcgt tagttgtgaa taagttgatc tgaaaggatg ttatgtacag      900

caagcagcag cagttaagag tctggggagt agctgcacag ggcgaggtgt caagatggga      960

agggtcctgc ctccttatgt gtttttccct gtaggggagg aagcctctta tgggcaatgg     1020

ttgggcatat tttccagcca gcccttcttt ctataggggc cagggtgggc ccagctcgtc     1080

ttggcttcca ccaccaggag agtgagggca ttgaagggcc ataaatagtc ctcccatcta     1140

cgtgcaccag agggtgtcgt ctaggctgtg catgccacga ggggaaggag ccaagaatga     1200

gtgtatgggt tgttttcatg tttaggctgg gataaaactg ttttcaattg cgcctgccgg     1260

gtgaaaacca cagcagcatc agcaagcttg gagaaggcca gcccgcccag cacaggctca     1320

cgttcccact caggcggtca gtcgggcggg ggtgtgagtc aggcaggcga gggtgtctgt     1380

gcctgacatc agcacctctg cttagccact gcagcccctg gagcagggta gggcgtcatt     1440

tgcagcaatc acctgctgcc tcacacgtcg cagcttggaa tttcaacgac catcagcgct     1500

ggggttgttg agggatcata gcagattttg gtgcagcctg gttgtcatgc tctttgtgga     1560

atggcctcta tgttcgagca attcgttgga tgttgaggtg cttggggaca gagagtcgaa     1620

tgatgggcca gggtcaaaca tgcgagcgtt tggctgagtc agcggttttt gctggtcact     1680

ttttcttttg tttcttattt aggtttgatg gatgtgtttt gtgctgctgc cctgaagctg     1740

cagcagcgtg tctgccctgc gctactgcgg gcaccaaggc tatgtgctgg tgcactcggc     1800

tgcgctgcac ctgtgcacct cgcactccgt ccagcctcca tgcagcacac gtactcacgg     1860

tgtcctcctg acctgtcgta cgctattcca aacttgctct tttgctgccg ctgctctcgt     1920

acacaattgc tgttgattat cgatatctaa tcgagcgcct gctgactgaa ctccgcaggt     1980

ttggatgaac tgtatgcaga gtggtctgaa gtggtgagca ccaactttag gtgggtgggc     2040

tctgaaggag gaggagggag cgggtgatta aacagggcct gcatgaagag gagcaggggc     2100

tgcatggaca gcagggggaa ggtgcagaag ggagggtcaa gcggggttca ggtggctgtg     2160

ggtttctgca cgagcagtga aagaagctgt atccttccac ctgctttcac tggcgaaagg     2220

ttgaaaacag gatgtcgcag ctggaaagat gttgcgctgt caagtgcaag ccatggttga     2280

gggtatgcct gtgtgcatgt gcttcttaaa gttactcctg ttctatggtt ctgggtgctt     2340

gttgtttgtg gtgcagggat gcaagcggac ctgcaatgac agagattgga gaacaacctt     2400

ggggaaggga gtttgcattg agagatcctg caggtgaggg ggcatgtaag caatggcagg     2460

caattcaaga acgaatcatt gctgcaaatg ctgggatggt atgcagctga ggtatctatt     2520

gccttgtatt ttgtctcgca ttgcatcggt ggtgcgttct gtggcctgag gcacagttct     2580

tgctgtttga taagggttcg actgagttgt cgtgtgtgct gtgctgcagg caattgcgtg     2640

cactttgttg cagaagaaca ggactga                                         2667


<210>  67
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AE596 Primer

<400>  67
tgcgacatgc agcttactaa cctgctcgac at                                     32


<210>  68
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AE597 Primer

<400>  68
atgggctcct tgatgttgtc cgccgtta                                          28


<210>  69
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AE405 Primer

<400>  69
acccaaaccc atgccagtgt a                                                 21


<210>  70
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AE406 Primer

<400>  70
actgtatgca gagtggtctg aagtg                                             25


<210>  71
<211>  4402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dual Promoter HygR cassette

<400>  71
cgtgcaggtg tacagattga aggaaacaat ggagatatct ttggcagttg aaaaccgtgt       60

tcgaatcatg cttttctact ctccaactga gacgaaattt atagcgccat gtcgcttctg      120

actaccaggc ttaggaaggc ctcatcacaa gctggatcgg ttcgaattaa gcaggcactg      180

aagccaagct tgcaagacag ccacctttta attccctcaa aacactttct caattcagcc      240

cggtaaatat gccgattcac agcggccaag atagagggga ggttagcaag aatgttgcga      300

tccctcccca gtcgttgcct cgcacacaac ctaggccttc acctttccat ggaaaattga      360

gaagtgaata ttggttttct tacggcatat cagatgaaat catgacccct aaacatgaag      420

agctgcaggc aaaacacctg ctctggacga gcacgatgaa atctcgagaa cccgccgtac      480

ttcagttgat cccgcatgat gacggccgcc attgaaataa gccacctcac tttattctag      540

caccgatttc caccgttgtg agggccgaac gaggacaatt tcgtgcgaaa caagcacgaa      600

cacgcacacg attagtagta cagacgagca gatcgatggc atgcggcacg gtctcgcgtt      660

ctcggcgacc aggacaacgg agcagaggga ggcctgccga gttccgaggg gcattttagt      720

ccaaaattgt gttgacacgt gaacaagtgg cttgaaaaga ggaaggaaat gcctgggttt      780

cccttcgaga gcgggaactc gcttgtgcgt catcctagct acccatggtc cctttgtggg      840

ggaggctgtt tcgtcctacc gaatgtgtgg cgctccatgc atcttctgcc tcccaaacca      900

ccaacatgag cacgcgaagg aaggagaaaa aagtggccgc aacgttctct tctcatattt      960

attgtctcat cacaaacata ggtacataat acaacaatca tgtccacagc ccgaacccat     1020

gagagagaat cataatcaaa gatgagccag ccacgaagct accggagaat tctgtaagaa     1080

aaatgtttaa agttgaaaat gctaacagtg aagtgatatc cttttttaat ggagtgttga     1140

ggtgaagtct agcatcgtag gggaaaacag gattctgtgt cttccattct actccttgat     1200

aaagcgaaga aatccgacaa aaccaaagag attgttcaag tttaagattt gtaagcgtac     1260

aactatgaac ttcttctctt tgtaggcctg agtggtcgta tgcatacgat tcatgaagtg     1320

aatcagtatc gctggatttt gcttaggagt aaagcacaac taagaaaata tgctgcctgg     1380

caggcatcct gagacatgag gcaagcgacg tagcaattga atcctaattt aagccagggc     1440

atctgtatga ctctgttagt taattgatga accaatgagc tttaaaaaaa aatcgttgcg     1500

cgtaatgtag ttttaattct ccgccttgag gtgcggggcc atttcggaca aggttctttg     1560

gacggagatg gcagcatgtg tcccttctcc aaattggtcc gtgtggtagt tgagatgctg     1620

ccttaaaatt ctgctcggtc atcctgcctt cgcattcact cctttcgagc tgtcgggttc     1680

ctcacgaggc ctccgggagc ggattgcgca gaaaggcgac ccggagacac agagaccata     1740

caccgactaa attgcactgg acgatacggc atggcgacga cgatggccaa gcattgctac     1800

gtgattattc gccttgtcat tcagggagaa atgatgacat gtgtgggacg gtctttacat     1860

gggaagaggg catgaaaata acatggcctg gcgggatgga gcgtcacacc tgtgtatgcg     1920

ttcgatccac aagcaactca ccatttgcgt cggggcctgt ctccaatctg ctttaggcta     1980

cttttctcta atttagccta ttctatacag acagagacac acagggatca tggggaagaa     2040

accggaactg accgctacgt ccgtggagaa attccttatt gagaagttcg actctgtctc     2100

cgacttgatg caactgagcg agggagagga gagtagggcg ttctcgtttg acgtaggggg     2160

tcggggatac gtgttgaggg ttaatagttg tgcggacggg ttctacaagg atcggtatgt     2220

ctaccgtcat ttcgcctccg ccgctctccc cataccagag gtactggaca ttggggagtt     2280

tagcgaatct ctcacgtact gcatctcgcg ccgagcccag ggagtgacgt tgcaagatct     2340

gcccgaaact gaattgcctg ccgttttgca acccgtggcc gaggccatgg acgcgatcgc     2400

tgccgcagat ctgtctcaga cgtccggctt tggacctttt gggccccagg gcatcgggca     2460

gtacacgacc tggcgagact tcatctgcgc cattgccgat cctcacgtct atcattggca     2520

gacagtcatg gatgacaccg tgtctgcatc cgtggcccaa gcactggacg aactcatgtt     2580

gtgggccgag gattgccctg aggtcaggca cctggtgcac gcggatttcg gcagcaataa     2640

cgtacttaca gacaatggtc ggattactgc tgtcatcgac tggtccgaag cgatgtttgg     2700

tgatagccaa tacgaagtgg cgaacatatt cttctggcgt ccctggttgg cgtgcatgga     2760

gcagcagaca cgctactttg aacggaggca cccggagctg gccggctccc cacgactccg     2820

cgcctatatg ttgcgtatcg gactcgatca gctttaccag tctctcgtcg acggcaactt     2880

cgacgacgcc gcgtgggcgc agggccgctg cgacgcgata gtccgcagcg gggctgggac     2940

ggtgggtcgg acccaaatcg cacgccggtc ggctgcggtg tggacagacg gctgtgttga     3000

ggtgcttgcg gactcgggca accgtaggcc gagcacccga ccgcgtgcaa aggagtgatt     3060

gaatcattga atgaaccatt gtgtgcagaa tcgatttcgg gagtgttgcc aacacaagaa     3120

atatgcccag ggttgtgtag aagtttgcgt gaatgtgatg aagggaagcc atacgctgaa     3180

ttatcgtgac gtgtgtgaga cgaagtgtca catcatacac ccaatttgag aagctgtacc     3240

tattagaaga atttgtgaga tacattaaac cccttttggt acgtggtata attgttattt     3300

gggaagctgt aaacacgcag atcgttcctg agattgtcaa ttacttttgt ggtgtttcct     3360

aaaggccgca tcactgcccg aatcgagttg atggcccgca aaaataagca tacatcatat     3420

gaatacaatt cagcttaaat ttatcataca aagatgtaag tgcagcgtgg gtctgtaacg     3480

atcgggcgta atttaagata atgcgaggga ccgggggagg ttttggaacg gaatgaggaa     3540

tgggtcatgg cccataataa taatatgggt ttggtcgcct cgcacagcaa ccgtacgtgc     3600

gaaaaaggaa cagatccatt taataagttg aacgttattc tttcctatgc aatgcgtgta     3660

tcggaggcga gagcaagtca taggtggctg cgcacaataa ttgagtctca gctgagcgcc     3720

gtccgcgggt ggtgtgagtg gtcatcctcc tcccggccta tcgctcacat cgcctctcaa     3780

tggtggtggt ggggcctgat atgacctcaa tgccgaccca tattaaaacc cagtaaagca     3840

ttcaccaacg aacgaggggc tcttttgtgt gtgttttgag tatgatttta cacctctttg     3900

tgcatctctc tggtcttcct tggttcccgt agtttgggca tcatcactca cgcttccctc     3960

gaccttcgtt cttcctttac aaccccgaca caggtcagag ttggagtaat caaaaaaggg     4020

gtgcacgaat gagatacatt agattttgac agatatcctt ttactggaga gggttcaagg     4080

gatcaaatga acagcgggcg ttggcaatct agggagggat cggaggttgg cagcgagcga     4140

aagcgtgtcc atccttttgg ctgtcacacc tcacgaacca actgttagca ggccagcaca     4200

gatgacatac gagaatcttt attatatcgt agaccttatg tggatgacct ttggtgctgt     4260

gtgtctggca atgaacctga aggcttgata gggaggtggc tcccgtaaac cctttgtcct     4320

ttccacgctg agtctccccc gcactgtcct ttatacaaat tgttacagtc atctgcaggc     4380

ggtttttctt tggcaggcaa ac                                              4402


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACCase target sequence 1

<400>  72
gggaaactgt gtgagaggag                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACCase target sequence 2

<400>  73
ggggaggggc ttccaccaca                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACCase target sequence 3

<400>  74
ggtgcgtcgc caagactgcc                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACCase target sequence 4

<400>  75
ggcgagctct cttttccgtg                                                   20


<210>  76
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WX120-Accase Forward primer

<400>  76
ccttgatggt gctatatg                                                     18


<210>  77
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   WX107-Accase Reverse primer

<400>  77
ttggagacga agagtgcc                                                     18


<210>  78
<211>  18
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  ZnCys-2845 gene target sequence

<400>  78
agtaggccat tcccggag                                                     18


<210>  79
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide for producing chimeric guide targeting 
       ZnCys-2845, first strand

<400>  79
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aacctccggg aatggcctac tcctatagtg agtcgtatta      120


<210>  80
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide for producing chimeric guide targeting 
       ZnCys-2845, opposite strand

<400>  80
taatacgact cactatagga gtaggccatt cccggaggtt ttagagctag aaatagcaag       60

ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt      120


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-ZnCys-Forward Primer

<400>  81
acctccttgt cactgagcag                                                   20


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-ZnCys-Reverse Primer

<400>  82
gatcccaaag gtcatatccg t                                                 21


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-1 CRISPR target sequence

<400>  83
ggctgtcaaa tcaacaaaac                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-2 CRISPR target sequence

<400>  84
ggagctcaga tatcttccag                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-3 CRISPR target sequence

<400>  85
ggatcttcca gtggtgggcg                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-4 CRISPR target sequence

<400>  86
gggggactgt cccattgtgc                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-5 CRISPR target sequence

<400>  87
ggtctgtcta aatcagcaca                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-6 CRISPR target sequence

<400>  88
gggccaagtg catcatgctc                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-7 CRISPR target sequence

<400>  89
gggctcaggt acgcatctca                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-8 CRISPR target sequence

<400>  90
ggattggaat caattttgaa                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-9 CRISPR target sequence

<400>  91
gggctgttca tcacaaagag                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-10 CRISPR target sequence

<400>  92
ggctctttgt gatgaacagc                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-11 CRISPR target sequence

<400>  93
ggcgtcggtt cacgccaatc                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bash-12 CRISPR target sequence

<400>  94
ggaactcgct cgtcgatcac                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-5'Bash-ZnCys Forward Primer

<400>  95
tagcagagca ggctcatcac                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-5'Bash-ZnCys Reverse primer

<400>  96
gaatatgtgg tctagctcgt                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-3'Bash-ZnCys Forward Primer

<400>  97
atggctccac cctctgtaag                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MA-3'Bash-ZnCys Reverse primer

<400>  98
ctgactacag ctagcacgat                                                   20


<210>  99
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZnCys-2845: Forward primer

<400>  99
atacaggaag cgtggttaca g                                                 21


<210>  100
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZnCys-2845 Reverse primer

<400>  100
gaagtattaa gggactggcc g                                                 21


<210>  101
<211>  849
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  1T5001704 Housekeeping gene

<400>  101
atgtcacggt cgcggtcctg ttccgaagct tctgcggcct cttcgtcatc ggcagcagca       60

gcgtcttcga ctcacgcccc ttcttcgcgc ggagcttcgg tggccgacgg tgctgcaagg      120

gagcgagaag ataatggcaa acgcctgagg tcaccgagcc ctgccggtgg tgaagcttct      180

ggttccgagg aagcggaaga ggatgatgag cccgccaaat tgcatgtttc tggtctaaca      240

agaaacgtga cagaggagca tctcaacgag atattcgcca catttgggaa gctgtcgcgt      300

gtggaactgg tacttgaccg acgagtgggc ttatcgcggg gcttcgccta tgttgagtac      360

gatcatcgga aggacgctga ggaagcccag ctgtacatgg acggtggtca gcttgacggc      420

gcacctttga aagtgaactt tgtgcttttg ggcggagccg cagccgatct cctgtatccc      480

gtggcggtgg tcgagaaagg gacctttacg atcgcaatgg cggtccgccg gagaggaggg      540

gcgggggagc tcaatgggag gggcggcggg gccggtctcg ttctccgcct cgggggggtc      600

gacacgaccg aggtcggttg ccgccagggc ggtttactcg aggagagcgc ggacgcagcc      660

ccccctaccg tcgccagcca gaccctcgcg gctggtcgcc gccacggcgc gggccgggtg      720

ggcgggcatc tccgcctcgg gccgcggtcg cagccggagc agccgctcct cacgcagccg      780

ttcctagatg gagggggcgg cgccaacagg gaaggcaagc aggagtctca cccgccgctt      840

gaggcttga                                                              849


<210>  102
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region of ZnCys-2845 gene targeted by RNAi

<400>  102
gtttaaacga tcagccacga cggctctcgc atgcacaacg atggcatgga ttggcgcgcg       60

aaggatgagg actgctcgta ccacaccgcc gtggacgcca gctgccacat cgacagtagc      120

taccaccatg ttgatgcctc aggccactcc atggtcgacg cctcgggtca cagcacgata      180

gacgcgtcgg gccacgactc cctcatcgac tcaagcggcc attacgacga ctatctggcg      240

cacaaggggg acgcccgcta catggagcac agttgtgaag cctgcaagcg ctcgaagaaa      300

cgatgcaacc gccgcaaccc ctgccagatc tgcacctcca ggggcatcaa gtgtgtgccg      360

caaatccggg gtcctgggcg ccccccgggc agtaaaagca gtcggggc                   408


<210>  103
<211>  163
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator 9

<400>  103
gagtcaaggg ggaaggtgca tagtgtgcaa caacagcatt aacgtcaaag aaaactgcac       60

gttcaagccc gcgtgaacct gccggtcttc tgatcgccta catatagcag atactagttg      120

tacttttttt tccaaaggga acattcatgt atcaatttga aat                        163


<210>  104
<211>  22313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSGE06337-Asc/Not Fragment


<220>
<221>  misc_feature
<223>  genes with promoters and terminators

<400>  104
gcggccgccg tatggtcgac ggttgctcgg atgggggggg cggggagcga tggagggagg       60

aagatcaggt aaggtctcga cagactagag aagcacgagt gcaggtataa gaaacagcaa      120

aaaaaagtaa tgggcccagg cctggagagg gtatttgtct tgtttttctt tggccaggaa      180

cttgttctcc tttcttcgtt tctaggaccc cgatccccgc tcgcatttct ctcttcctca      240

gccgaagcgc agcggtaaag catccatttt atcccaccga aagggcgctc ccagccttcg      300

tcgagcggaa ccggggttac agtgcctcac tcctttgcac gcggtcgggt gctcggccta      360

cggttgcccg agtccgcaag cacctcaaca cagccgtctg tccacaccgc agccgaccgg      420

cgtgcgattt gggtccgacc caccgtccca gccccgctgc ggactatcgc gtcgcagcgg      480

ccctgcgccc acgcggcgtc gtcgaagttg ccgtcgacga gagactggta aagctgatcg      540

agtccgatac gcaacatata ggcgcggagt cgtggggagc cggccagctc cgggtgcctc      600

cgttcaaagt agcgtgtctg ctgctccatg cacgccaacc agggacgcca gaagaatatg      660

ttcgccactt cgtattggct atcaccaaac atcgcttcgg accagtcgat gacagcagta      720

atccgaccat tgtctgtaag tacgttattg ctgccgaaat ccgcgtgcac caggtgcctg      780

acctcagggc aatcctcggc ccacaacatg agttcgtcca gtgcttgggc cacggatgca      840

gacacggtgt catccatgac tgtctgccaa tgatagacgt gaggatcggc aatggcgcag      900

atgaagtctc gccaggtcgt gtactgcccg atgccctggg gcccaaaagg tccaaagccg      960

gacgtctgag acagatctgc ggcagcgatc gcgtccatgg cctcggccac gggttgcaaa     1020

acggcaggca attcagtttc gggcagatct tgcaacgtca ctccctgggc tcggcgcgag     1080

atgcagtacg tgagagattc gctaaactcc ccaatgtcca gtacctctgg tatggggaga     1140

gcggcggagg cgaaatgacg gtagacatac cgatccttgt agaacccgtc cgcacaacta     1200

ttaaccctca acacgtatcc ccgaccccct acgtcaaacg agaacgccct actctcctct     1260

ccctcgctca gttgcatcaa gtcggagaca gagtcgaact tctcaataag gaatttctcc     1320

acggacgtag cggtcagttc cggtttcttc cccatcgagc tcggtacccg gggatccatg     1380

attgttgtat tatgtaccta tgtttgtgat gagacaataa atatgagaag agaacgttgc     1440

ggccactttt ttctccttcc ttcgcgtgct catgttggtg gtttgggagg cagaagatgc     1500

atggagcgcc acacattcgg taggacgaaa cagcctcccc cacaaaggga ccatgggtag     1560

ctaggatgac gcacaagcga gttcccgctc tcgaagggaa acccaggcat ttccttcctc     1620

ttttcaagcc acttgttcac gtgtcaacac aattttggac taaaatgccc ctcggaactc     1680

ggcaggcctc cctctgctcc gttgtcctgg tcgccgagaa cgcgagaccg tgccgcatgc     1740

catcgatctg ctcgtctgta ctactaatcg tgtgcgtgtt cgtgcttgtt tcgcacgaaa     1800

ttgtcctcgt tcggccctca caacggtgga aatcggtgct agaataaagt gaggtggctt     1860

atttcaatgg cggccgtcat catgcgggat caactgaagt acggcgggtt ctcgagattt     1920

catcgtgctc gtccagagca ggtgttttgc ctgcagctct tcatgtttag gggtcatgat     1980

ttcatctgat atgccgtaag aaaaccaata ttcacttctc aattttccat ggaaaggtga     2040

aggcctaggt tgtgtgcgag gcaacgactg gggagggatc gcaacattct tgctaacctc     2100

ccctctatct tggccgctgt gaatcggcat atttaccggg ctgaattgag aaagtgtttt     2160

gagggaatta aaaggtggct gtcttgcaag cttggcttca gtgcctgctt aattcgaacc     2220

gatccagctt gtgatgaggc cttcctaagc ctggtagtca gaagcgacat ggcgctataa     2280

atttcgtctc agttggagag tagaaaagca tgattcgaac acggttttca actgccaaag     2340

atatctccat tgtttccttc aatctgtaca cctgcacggt gcaccagttg gtacggcata     2400

ttatggtttg aatgctggcg acctcatgaa ctttgttgat ttttttagaa ttgtgtcatc     2460

gaaaaatata caatgtcgaa gtaaagttta tacactctgg gtactcttct tctttgccat     2520

ccagtaggtg tgggatagga gtgcgtggta attctctgag acagttttcc taccatgttt     2580

cgggtccaca tagtacaagt tgttactcgt gacacctcat ttcgcttttg tttctccact     2640

aatggactac atttttcaaa gcagcagcgt ccgcgtttct cgattaagaa tctcccgtcc     2700

gctttgagtt tgtttatttt aaggattaga aggataaaag cggagctgca aaggtgaaac     2760

atgcctgaga ggactagaag acatacggag ctgaagtgcg gggaggttaa acccgatgat     2820

cccattcctt gcatttgtca aggctcaccg cattcatagt tctaaaaaga aaatatttcc     2880

gaataatgtt aacacaaaaa taattaaata ctgttctatg tactccgttc atttgttgat     2940

ggaatcaaaa ggccagaaaa aaagaggggg ggactccaag ctgccgcaga cgctcgcctc     3000

gtctctcgtt cgcggaatgc cggtcgtgga accgctgtga agataccgtg ccttggggat     3060

caattcacac attgacgagg gcgcctcacg ccgtggcgta cttattcggg ccaaaatgtt     3120

atttccaagg ggtgacggat ggcatgctcg actcgtgtga tggctgatgc atcaaccctt     3180

tcaatgctcc ttcctacttc ctcttatcca ctggtagtct gtcatgagat gtggtgaacc     3240

gcgtccgtga taatagtgtt ctcatttaac ctaacaacca acacaataca aaacttgatt     3300

agatacccag tgcatcgaat tttgggtgcc ttcaccgaca agcttgcact ttctcgaggg     3360

acgacaacaa caaaccacct acagcattac cgggcaatag cagcacaaca tggaggacat     3420

cgtgattgcc ggaatttccg gaagattgcc tgagagcaac aacttggagg agttttggca     3480

gaacctcttc aatggcgtgg acatggtgac agaggatgac cgaaggtgga aacctggatt     3540

gtacggattg cctagacgga atgggaagtt gaaggagatt gaccggtttg acgccgcatt     3600

ttttggcgtg catcctaaac aagcccatac gatggaccct caattgcggt tgatgttgga     3660

gatcagttac gaggcgattg tggatggagg aattaatccc gtgagcatga gagggagcaa     3720

aactggcgtg tacatcggag tgagcggatc tgaagcagga gaggcctttt ctaaagaccc     3780

tgaagagttg ttgggatact ccatgacagg atgtcaacga gccatgttcg ccaataggct     3840

cagctacttc ttcgacttta acggacctag cacggcaatc gacacagctt gttcttctag     3900

cttgttggcc ttggaaaacg ccttcaatgc catccggcac ggacaatgtg atgccgcctt     3960

gattggagga gtgaatttgt tgttgaagcc caatacctcc gtgcaattca tgaagctcgg     4020

gatgttgtcc cctgagggaa cctgtaagtc ctttgatgcc tccggaaatg gatactgtcg     4080

gtctgaagca gctgtggctg tgttgttgac gaaaaagtct atggcaaagc ggatctacgc     4140

caccgtgttg aatgcgggga acaatacaga tggatacaaa gagcaaggag tgacctttcc     4200

tagcggagag atgcaacaac gattggtgcg gtccctttac caagaagcca acatctcccc     4260

tgagcaagtg gagtacattg aggcccatgg aaccggaacc aaagtcggag atcctcaaga     4320

agtgaatgga atcgtgagcg tgttttgcca atcccctaga gatcctttgt tgattggatc     4380

caccaagtcc aatatggggc accctgaacc tgcatctgga ttagccgcat tggccaaagt     4440

cgtgttgtca ttggagcatg gagtgtgggc acctaacgtg cactttaacg agcctaaccc     4500

tgacatccct gccttgacag atggaagatt gtgtgtggtc tccaaaccta cacctgtgag     4560

aggaggaatt gtcggaatta attcctttgg atttggggga tcgaacgtcc acgtcatctt     4620

gtctccccat gtgagcgact cttctgacaa aacacctgcc ccttctgtgc cccgattgtt     4680

gcaagcttct ggacgaactg aagaggccgt tacagccttg ttttctaatg cccaacaaca     4740

ccaagagaac tcctcctact tgtccctgtt gaatgatgtg tctggtgtgc ctactgctgg     4800

aatgccttac cgaggataca ctttgattgg cgcccaagga gaattgaccg aagtccaaca     4860

gacccaacct acgcctcgac ctttgtggta catctgctcc ggaatgggaa cgcaatgggc     4920

tggaatggga caatctttga tgcaattgtc tgagtttcga gagtccatta aaagatccga     4980

tatcgccctg aaggacaccg gattgtgcgt gtctcgattg ttgatggagg ccgatgagag     5040

cacctttgaa gacaccgtcc atgcatttgt gggattggcc gccattcaag tggcccaaat     5100

cgacatgttg cggaagatgg ggttggagcc taatggaatt gtgggacatt ctgtgggaga     5160

attggcatgt ggatatgctg atggatcttt gagccattcc gaagccattt tggcagcata     5220

ctggcgagga agatgcatta aagaggcgaa cttgccccct ggaggtatgg cagcagtggg     5280

attgacatgg gaagagtgta aagcgcaatg ccctcaagga gtggtgccag cttgccataa     5340

tgcagaagat acagtgacaa tttctggacc ccaagactcc gtgtccaaat ttgtggccca     5400

attgaaagaa tctggcgtgt ttgcgaaaga ggtgcgatct gctggagtgg cctttcattc     5460

gtactacatg gcctccattg cccctgcctt gttgtctgcc ttgcaaaaag tgatcaagtc     5520

cccccgccct agaacagcac gatggatttc tacatctatt ccccaatccg actgggaatc     5580

tcctcttgcc ttgtactctt ctgctgagta tcatgtgaac aatcttgtgt cccctgtcct     5640

cttccaagag gggttgaatc atgtccccga taatgccgtg gtcgtcgaaa tcgcccctca     5700

tgctttgttg caagccatcc tcaaacggtc cttgaagcct acctgttcca ttttgccctt     5760

gatgaaacgt ggccatgcca acaacctcga gttctttttg tcccatgtcg gaaaagtgta     5820

catgaacggg atcaacgtgg attccaacaa gctttaccct gccgtgaaat accctgtgcc     5880

tcgtggaacc cctctgattt ccccttacat ccagtgggat cactcccaat cctgggatgt     5940

gcctaaagtg gaagactttc ctgccggatc tggcggatct acatctgcaa ctgtgtacaa     6000

tatcgacatg aatcctgaat cccctgacta ctacatgatt ggacattgta tcgacggccg     6060

agtgttgtat cctgcaacgg gctacctggt gttggcttgg agaaccttga tgcgctcttt     6120

gggaacggtg atggaccata cccctgtgac gtttgaagat gtgaccatcc atcgagccac     6180

gatcttgcct aaaactggat ctgtgcaatt ggaagtgcgg ttgatgcctg caacaaatag     6240

atttgaagtg tccgagaatg gcaatttggc tgtgtccggg aaggtgtccg tgttggaaga     6300

ctctgggttg gatgcgtttc atgccgagct gaacaagccc attaccgctg acaatgaaga     6360

ccctaagctc cgcctgaaat ctggagatat ctacaaggag ttgcgattga ggggatacga     6420

ctacggaaag acctttcaag gaattttgga gagcaataat gccggagact ccgggaaact     6480

ccactggacg ggaaattggg tgacgttctt ggacacgatg ctgcagatga tcgtcgtggg     6540

attgcctgga agaagcttga gactccctac ccgaattcga tctgtgtgtg tggatcctaa     6600

attgcatgag gaacgcgtga atgactacga aggagaccaa aaagccgtga atgtttttgt     6660

gaatcggtgc ctcgacaaca tcacagccgg aggagtgcaa atctgtgggt tgcatgctac     6720

agccgcccct cgaagacaac aacaacaaac accccctacc ttggaggaat tcgtctttgt     6780

cccctacgaa gatgccgatt gcttgagaac caatgagaaa ttggccgacc aattgcgaca     6840

ctgtaaagga ttggtgcaac gattgcaacg caaattggcc aaacaaggcg tgaagatctc     6900

catccctggc ttggaaggag cgtccgaagg gcaattgatt gaagcagagg ccgagaaagg     6960

gttgttgagg ttgttgtccg tgttgtgcgg cttggagttg aatgggaatt tgagatccga     7020

attggaacaa acggtgcaaa aagagagaga ctgcttgctg caagatcccc tgttgaatgg     7080

cttgttggat tctcaagcgt tgcggcattg cctggatacc gcgctggaaa attccacacc     7140

tggcaagttc aaggtcttgg aggcccttgc tgctgatgga cgcgtgtttt ctcaagctgt     7200

gtctttgctg aatattcaac ctatgttgcg tttggactac actgcctctg atatttccgc     7260

cgaccaattg tccgcccaac aatcttcttt ggaagagcag ggaatctcta ccgcccagtg     7320

ggatcctctt caaggacccg tgacaggagg attggatgga gccgatcttg tggtgtgcaa     7380

ttgtgctgtt ggatctgcca cgaatcctgc attgttgatt gagaatttga catctgccgc     7440

tagagaagga ggattcatct tgttgcacac gttgttgaga ggagatacgt tgggagaaac     7500

agtggcgttc ctcacgagcc agaataaccg gaagggcctg ttgacgcaaa ccgagtggga     7560

agagctgttc caaaaagcct ccctgaatgt ggtgatgttg cggaagtcct attacggaag     7620

tgccttgttt ttgtgtcgaa gaagccaaca atccagccaa aagcaaccta ttcacatctt     7680

cgtggaccct accgactaca aatgggtgga gaccttgaaa tccacactcg ccgagtcctc     7740

cgatatcccc gtctggttga ttgccaccaa aggccataat ggagtcgtgg gaatggtgaa     7800

ctgcttgcgt caagagcctg gaggaaatag aattcgatgc acatttgtct ccaatttgtc     7860

caaaggagct gcagtgcctt ccttgttgcc taatgagaag gtgatgaaag ccctgttgaa     7920

gaaggacctg gtgatgaacg tccaccgcga cggactgtgg ggagtgtttc gacatcaatt     7980

gctgacccaa gacctgtccg aagaattgac cgagcaggcc tacgtgaatg tgttgacaag     8040

aggagatttg tctagtttga gatggattgc gtcccccttg aggcattttg tggcatcttc     8100

tcctaatgtg caattgtgca gagtgtacta ctcctccctg aacttccgcg acatcatgct     8160

cgccaccgga aaattgcccc ctgacgccat ccctggagat gtggcattgc agcaatgtat     8220

gttgggaatg gagttctccg gaagagatcc ttctggaaga agagtgatgg gattgttgcc     8280

tgctaaagga ttggccacat gtgtggatgc cgataaaaga tttttgtggg atgtgccttc     8340

ttcttggacg ttggagcaag ccgcctctgt gcctgtcgtc tacgctaccg cctactactc     8400

cttggtggtc agaggaagat tgagacctgg agaaagtgtg ttgattcatt ccggatctgg     8460

tggagttgga caagccgcta ttgcaattgc cttgagcatg cgctgtagag tgtttacaac     8520

agtgggatct aaagagaaga agcaatactt gcaagagcgc ttcccccagt tgacagccga     8580

gtccttcgcc aattcccgcg attcctcctt cgagcaacat gtgatgctga acacacaagg     8640

aaaaggagtg gacctcgtct tgaattccct cgccgaagag aaattgcaag cctccttgag     8700

atgtcttgcc agacatggac gatttttgga aatcggaaaa tacgatttgt ccaacaacac     8760

ccccctgggc atggccctgt tcctgaagaa cgtcgccttt cacgggatct tgctcgatgc     8820

actgtttgaa gagggcaatc gtgaatggga agaggtgtcc gacctgttga agaaaggaat     8880

ttcctccgga gtggtgcaac ctttgagaac gaccgtgttt gagagaaacc aagtggaaga     8940

agcatttaga tatatggcgc agggaaagca catcggcaag gtgttgctgc aagtgcgctc     9000

cgaagaatcc tcctcctctg gacctgcagt gtctgctttg agtattcctg ccatttgccg     9060

aacgttttgt cctgcaagtt tgtcctacat catcaccgga ggcttgggcg gatttggatt     9120

ggaattggcc caatggttga cagaaagagg agcaagaaaa ttggtgttga cgtctagatc     9180

cggaatccgc aacggctacc aagctaagag agtccgagag tggcaagcca tgggaattca     9240

agtgttggtc tccacatccg acgtgtctac cttggatgga acagaacgat tgatcacaga     9300

ggcctgtagg ttgggacctg tgggaggaat tttccatttg gcaatggtgt tgaaagatgg     9360

aatgctcgag aatttgaccc ctcaggagtt catcgaggtg aatcggccca agtacgacgg     9420

gacgatcaat ttggacagcg tgacccgaca aaaatgcccc caattgcagc aattcgtcgt     9480

gttttcttcc gtgtcttgtg gacgagggaa tgccggacag tccaattacg gatttgcaaa     9540

ctccacaatg gaaagaatct gcgaacaaag acgacaggag aatttgcctg gattggccgt     9600

gcaatgggga gccatcggag atgtgggagt ggtcttggag acaatgggag gcaatgatgc     9660

cgtgatcgga ggaacattac ctcaaagaat gtcttcctgc ttggaggtgt tggaccgctt     9720

tttgtgtcaa caacgacctg tgatgtcctc ttttgtgttg gcagaaaaag tggtggtgac     9780

aaaaggagag ggatccggac aaaaggacct ggtggaagct gtggcgcaca tcttgggagt     9840

gagggacgtg aatagcttga atgccgacgc atcattggcc gatttgggat tggatagctt     9900

gatgggagtg gaagtgagac aaaccttgga gagagactac gacattgtga tggctatgag     9960

agagatcaga caattgacga tcaacaagtt gcgggagttg agcaagcaat ccggcgggaa    10020

ggaggaatcc cctgtgaaga ggtctggagc ccaagcattg ttggaaagcg acttgtcccg    10080

aatgttggtg aatcctgacg gacctacaat ggcacccttg aatgaagtcc aatccgcaga    10140

aagacccttg tttttggtac atcctatcga aggatctatt gccgcatttc gaaccttgac    10200

agcgaagctc agcgtgccct gctacggatt gcaatgtacc aaagccgccc ctttggactc    10260

tatccaatct ttggccgcct actatgtcga atgtgtgagg caagtgcagt tggaaggacc    10320

ctacagaatt gccggatact cctttggagc ttgtgtcgct tttgaaatgt gttcccaatt    10380

gcaattggcg aaatgccctg tggagtacct gttcctgttc gacggatccc actcttacgt    10440

cgccgcgtac actcaatctt atcgagccaa gttgaccccc ggaaaagaag ctgaagcaga    10500

aacagaagcc ttgtgtgcct ttatccagca gttcaccgga atcgagtaca acaaactctt    10560

ggagaccttg ttgcctttgt ctgatttgga agccagagtg gacaaagcag tggacctgat    10620

cacctcctcc cacaagaacg tgtcccgcga tatgttgcat tttgccgcct ccacgtttta    10680

ccacaagttg aaagccgccg acagatacgt gcctacatcc aaataccacg ggaatgtgac    10740

cttgttgaga gccaaagcat cttctgagta tggagacgga ttgggatctg actacaaatt    10800

gcacgaagtc tgtgacggga aggtgtccgt gcatgtgatc gagggcgacc atagaacctt    10860

tttggaggga gaaggagtgg agtctatttc cagcatcatc cacagcagct tgtctgagcc    10920

tagagtgtcc acgagagaag gataaagtga tgcggccttt aggaaacacc acaaaagtaa    10980

ttgacaatct caggaacgat ctgcgtgttt acagcttccc aaataacaat tataccacgt    11040

accaaaaggg gtttaatgta tctcacaaat tcttctaata ggtacagctt ctcaaattgg    11100

gtgtatgatg tgacacttcg tctcacacac gtcacgataa ttcagcgtat ggcttccctt    11160

catcacattc acgcaaactt ctacacaacc ctgggcatat ttcttgtgtt ggcaacactc    11220

ccgaaatcga ttctgcacac aatggttcat tcaatgattc aagtacgttt tagacggact    11280

aggcagttca taatcaaaga tgagccagcc acgaagctac cggagaattc tgtaagaaaa    11340

atgtttaaag ttgaaaatgc taacagtgaa gtgatatcct tttttaatgg agtgttgagg    11400

tgaagtctag catcgtaggg gaaaacagga ttctgtgtct tccattctac tccttgataa    11460

agcgaagaaa tccgacaaaa ccaaagagat tgttcaagtt taagatttgt aagcgtacaa    11520

ctatgaactt cttctctttg taggcctgag tggtcgtatg catacgattc atgaagtgaa    11580

tcagtatcgc tggattttgc ttaggagtaa agcacaacta agaaaatatg ctgcctggca    11640

ggcatcctga gacatgaggc aagcgacgta gcaattgaat cctaatttaa gccagggcat    11700

ctgtatgact ctgttagtta attgatgaac caatgagctt taaaaaaaaa tcgttgcgcg    11760

taatgtagtt ttaattctcc gccttgaggt gcggggccat ttcggacaag gttctttgga    11820

cggagatggc agcatgtgtc ccttctccaa attggtccgt gtggtagttg agatgctgcc    11880

ttaaaattct gctcggtcat cctgccttcg cattcactcc tttcgagctg tcgggttcct    11940

cacgaggcct ccgggagcgg attgcgcaga aaggcgaccc ggagacacag agaccataca    12000

ccgactaaat tgcactggac gatacggcat ggcgacgacg atggccaagc attgctacgt    12060

gattattcgc cttgtcattc agggagaaat gatgacatgt gtgggacggt ctttacatgg    12120

gaagagggca tgaaaataac atggcctggc gggatggagc gtcacacctg tgtatgcgtt    12180

cgatccacaa gcaactcacc atttgcgtcg gggcctgtct ccaatctgct ttaggctact    12240

tttctctaat ttagcctatt ctatacagac agagacacac agggatcatg gatggagtga    12300

gatgggcctt tagatgtgga tcttgggtgc cttccagatc tgagtggaca ttggcagcaa    12360

gatgtgtgca acaagaggag aagttgagga tcggacagtt cgtgtttgcc aaagacgcca    12420

aatctgcaat ggccggacga ttgttgatca ggaagttggt gtgcgagaaa atggggtttg    12480

cctgggacgg atttaggttg caaagaacag agcggggaaa accttacttg ccccaaacaa    12540

gttccgcccc ttcttggagc tttaatgtga gccatcaagg cgattacgca gtgttagcag    12600

ccgaagctgg aagacaagtg ggaattgacg tcatgaaaac gtccagacct ggaagctctt    12660

ctgtgcaaga gttcttccgc atcatgaatc gccaatttac cgacctcgag tggaccaata    12720

tccgaaccgc aggatctgac tgggaccaac tgcatatgtt ttaccgccat tgggccttga    12780

aagagtcctt caacaaggca atcggaaccg gattgggctt cgacttgcaa cgtgccgaat    12840

ttcatatctc ccccaaccaa atgcgagaag gccaagtgta ccgacaaaca agaatgtacc    12900

tcgactccga agaagaagaa gactggacct tcgaggagtc cttgctcgac aaagatcacc    12960

atgtggctgt ggccttggga aaacctgaca tctctatgtc caagaaagac ggaggatcct    13020

cctgtgaggc accacctgca gcttttacag tgttgtcctt ttccgacttg gtgtcccaag    13080

caacacctct tttggacgaa gaccctacat attgggagga gtttcagaag aagaaggagg    13140

ccccttcccg acaatccgat caagagtaac cgtcaaaggc aaaggaggaa aagaagaagg    13200

cttacaccag tggcaccgaa atgttaatag cttgatgttt gcataaattg tcaaagtaaa    13260

tgaaggtcta gagcttgaag gttgcatctg aaaaaaatca acaacagatg gcgaaatcga    13320

tcgcatgctg accgtcaggc cttcagaatt accatcgcac tgcatagtcc gagcaggatg    13380

tcaatatagg gccgtcgtta aggatacgta ggccgttgct gtttgctgcc gcctgtggcc    13440

cgttcatcga atgaaaaaaa atgtgtatgt tcaagttaat ggttactaaa tggttcgcaa    13500

ggccgagttt cacagagcgg cagaagacat ccattgaaaa ctcgcaagta tgcaggagga    13560

atgaatatac tcagggtacg tgtatattta aaactattgt gaagcattag gatagaaagg    13620

aaggatctgc tacaaagtaa attcaggaac aatgtcggac acaccaaaga ttccgaccct    13680

gactgcccgc aaaaatggac gctattccaa aaacataaag gtaatgatac aaccccacgt    13740

accttggcat ttacgcatcc catacttatt tgtttggcac ccgttggtgt gttagtgggt    13800

agcgcttgtt cttactttcg ttcctgaacg cctgaaagcc aggaagagag gcacgatttg    13860

agtctttata ggggtcctcc ccggccaggc aacgcttctc tcccccagct gctctcgcaa    13920

aatttctctg agggctgtca tagctctgcg catcccctta tgtgacgtcg ccaccggtag    13980

atttcacgcg gacacgggca tatctcccat gtgcgggagg aacaaatgac gatggggtcg    14040

cattccatca tagacttggg ggcctctccc gtcatccagc tctgcgtcca gccttccggc    14100

actttgtctt gtgctcgtcg agcatcttcc ttcactccga cgcccatggt tcgcttgcgc    14160

gcttgcgttc gttgtccctt gggtacgaag gcgatgctgt tgtaatgaac gtcgataccc    14220

cggtggaggg atgggcgaag tttgtgaaag cgaggagagg tcatatgcag caggaagaga    14280

gagccaaccc gccctctccg attcgagatg aacccttcta aagctgtgtg tcactcatgc    14340

tcatgcattt ccctacaatg cccatctata atgcgcgaat agagaagaga tggccgctac    14400

tctccgaatg gatgaggcgg gagccaacga ggcagggggt cgtgttggtg gcgccggcgt    14460

ggaggaggtc cgggaacaag acagatattt gcccattgcc aacatcagtc ggataatgaa    14520

gaagtctttg gtaagtgtcg tgacgcatcc taatagtgcg ctccttctag atgtcccaac    14580

atctgtcgcc gatagacctt gttttgtccc acaagtctcc tgttcccatc ctcttctacc    14640

atgttctctt tcctcactca tgtgctccct ctctcgccac taatccgggc agccggcgaa    14700

tgccaaaata gccaaggacg ccaaagaaac cgtccaagaa tgtgtttcag agttcatttc    14760

tttcatcacc tccgaagcca gcgacaaatg ccaacaggag aagcgaaaga cgatcaacgg    14820

ggacgatctc ctctgggcca tgagcaccct gggcttcgac aaatacgtcg agcctctgaa    14880

acattatttg gtcaagtacc gtgagtcagt gaaggggggt gagaaggcgg acggagggaa    14940

gaaagggaag tctgagggca cccaagtgac aggaagtagc gcggcgggtc ctgtcacggc    15000

gaccgcgcta gctgagcctc agggtgacag tcgggtggaa tcgaccgaaa gcagtagtct    15060

ccccgagcag caacatcaca gttatgcagc ccgtgctgtg tcaaggcagc cgccttcctg    15120

aagacctgaa ggcgagacag taaattattt tcagagtcgt acaggaaatg gagagatgtt    15180

gcggaaaagt tatgccagta acgtacgcga cacacataca aactaaagat aaaagcaaaa    15240

ttgcccacgc acagtctaaa ttgaatattt tgctgcgaga ggctacatga tcaagtggag    15300

agcatccatt ttttgagccc catgaggttc attcaagtct acttaagttg tcttttttaa    15360

cttttcattg acatttttcg cctgttgtct cctctctgtg tcgatggcca ttcaataaaa    15420

gcactgtccc ggcattcaag agctgtagcg gtatcatgtc aacaatcagt aggaataagc    15480

atacatcata tgaatacaat tcagcttaaa tttatcatac aaagatgtaa gtgcagcgtg    15540

ggtctgtaac gatcgggcgt aatttaagat aatgcgaggg accgggggag gttttggaac    15600

ggaatgagga atgggtcatg gcccataata ataatatggg tttggtcgcc tcgcacagca    15660

accgtacgtg cgaaaaagga acagatccat ttaataagtt gaacgttatt ctttcctatg    15720

caatgcgtgt atcggaggcg agagcaagtc ataggtggct gcgcacaata attgagtctc    15780

agctgagcgc cgtccgcggg tggtgtgagt ggtcatcctc ctcccggcct atcgctcaca    15840

tcgcctctca atggtggtgg tggggcctga tatgacctca atgccgaccc atattaaaac    15900

ccagtaaagc attcaccaac gaacgagggg ctcttttgtg tgtgttttga gtatgatttt    15960

acacctcttt gtgcatctct ctggtcttcc ttggttcccg tagtttgggc atcatcactc    16020

acgcttccct cgaccttcgt tcttccttta caaccccgac acaggtcaga gttggagtaa    16080

tcaaaaaagg ggtgcacgaa tgagatacat tagattttga cagatatcct tttactggag    16140

agggttcaag ggatcaaatg aacagcgggc gttggcaatc tagggaggga tcggaggttg    16200

gcagcgagcg aaagcgtgtc catccttttg gctgtcacac ctcacgaacc aactgttagc    16260

aggccagcac agatgacata cgagaatctt tattatatcg tagaccttat gtggatgacc    16320

tttggtgctg tgtgtctggc aatgaacctg aaggcttgat agggaggtgg ctcccgtaaa    16380

ccctttgtcc tttccacgct gagtctcccc cgcactgtcc tttatacaaa ttgttacagt    16440

catctgcagg cggtttttct ttggcaggca aacatggaga cttcgacgag aaaaggcttt    16500

catgaacgca gtgacgcacg atttccagct ggagcgcttc atgattccat gaacttgaca    16560

tcaaatgatt cattcagaag ctctcagttt ggcaaggaag gcgccttcaa cgaagtcaag    16620

aaagaccctg gacgctggac ggctgaggaa catatgttgt ttcttagggg tctgcaatta    16680

cacggtaaat catggaagaa aatatccgag attgttacaa cgcggacagt ggtccaaatt    16740

cgtacacatg cccagaaata ccttataaag ttggaaaagg ctagaaaggc tggtcatcag    16800

ggtgtcttaa tgatggatgg gaaaggcgtc gataacactg aacgtcgagg cacttccaag    16860

aaatcatctt tgtccacgga aacggtgagt ttcacatcta cttctcctga atcttctgtt    16920

ctcgagcaga agagacagaa aaatgagcca gcggctcacc tgccaggtcc tgtgcagcat    16980

actactgtac gcccttttgc acctgtcaca agagccgcac cttcaggctg ccccccaact    17040

gcgcccgcag gattcgtgcc atggatggta ggcccatacg cttgtgtccc tccaacatac    17100

tacaacatgc aaatgatggc ccatggatat gactttggtg ctccgctggt atcgccatca    17160

tcacaatatc gtgcgagttt ttcaaatccc ctgtctgcac ccggaggaaa ttgccaagag    17220

caggacttgg gactaaacgc acctcaatct gtgagaatgc ccttggaggc tgaaatggct    17280

gcacaaattc attacctggg cattgacaac gaggaaacta tcgcgcaaaa tcagccaaat    17340

tcctataagc gtctacgtgt ccccgcaggt ctggctatgg cgacgcctgc cgcggtttct    17400

acggcacaat cctctcattg taaagtctac gttgccccgc agcagtcatg cacctcgcct    17460

tctgatatga ctccgactct aaccgcatca gccttacatc agccaccgca gggatctctt    17520

tgcccgttca tgacatcaaa tcctttagac gagctcatgc aaagcctgtc gcaggagaat    17580

ctttcttcct cctgctcctc gacctctcca tctatcgcac ccgaagttga tgtacccagc    17640

atgcatgaca tactcatttg ggacctaaat gggaaacata gccgcccttc ttcccttagc    17700

tcctgggacg aggagggctt cgatggagtc tcaacctcat cttcccctgc aagcattgac    17760

aatcacgttg tttgccaacc tccggatttg agcgagcttc agtatgcgaa agctgcgtga    17820

gagtcaaggg ggaaggtgca tagtgtgcaa caacagcatt aacgtcaaag aaaactgcac    17880

gttcaagccc gcgtgaacct gccggtcttc tgatcgccta catatagcag atactagttg    17940

tacttttttt tccaaaggga acattcatgt atcaatttga aatcaccaga tataggtgac    18000

ccgataacgg agccaataga gtccaggctt ttgcgtgtga cttgtacgtt tgggggcagc    18060

agcatagctg ggacggggtc gctcgtccct gtttcgagta tggcgcgcac tctgacgcct    18120

tcaagtgtgg ttgccatctt cgccgcaatc agctgagctg tcccgtcctc ataacccacc    18180

acgacagcta cttttcccat gccctccgct gacatcatca ctaagctttt ccctgccctt    18240

attgcacatg aatccttcag cgttgtccgt ggtggagcta gaaagcccca tgctccttgt    18300

tgcagcaaag tcagaaccgt gaagatcata aagccccaca atactgagaa atgcctgtgc    18360

catacacttt gtcttgcgcc tatcgacatt tttttactca ctatcactga tttggatccc    18420

agcttgtcct ccccgtcctg tatcgtgcat tgttgacttg agtttgatac cagaaatgct    18480

tcgaaagtag tcagtcagca tccatgcgag tgttacaaag gacatgtccg tgcgccgacg    18540

cgcttccatg caactttcac tgattggggc ttaaaaggag acaccccgcg gtacgcgata    18600

ccttgagacg gacgccggga gacggtctaa agcgggttcc caggggccag caagggggga    18660

ggtcgcttcc tgcgggggcg acggaggtga gcgctctctc cgtccatgtc cagccccgcc    18720

ccgccaggca ggtgtgatgc gggcgttgca tgcgtggtca ggcagcgaag agagcctgag    18780

acacctgcgc caaccactgc tgcctcaccc gtgcgcggtg gtggccgggc aagtgcgtgt    18840

aaggagggag ccggacatgg aggcgaggag gatgagagtg cacctcaagg ctctcctctt    18900

ctgctgtcca catcgcccaa ataaaaacca ccatccttta tatcattgga gcacatatcc    18960

tgttgaatca cagatcaacg ttttaaagag tcgtcaatcc gcgacgacag gagagggggc    19020

cgaagacagc gacgccacca tgtctatcag agggaagata tacctcattt ctggctccgc    19080

tgaaatcact gacctccccc gtggcgtctt cggtgttcga ggcggatgcg aagcagcagc    19140

aactcttgaa agattccctc accgcagacc ttaaactgct cttgcacgag tttgaacgct    19200

tccagcaagc gacagcatta gtgtcgagag agggctcgaa agaggtggag gcaatggagc    19260

gggcggcgaa agtggaattc ttcctaggct acatcggaaa ggtgcttcag gaacttgccg    19320

gcgccgacgc accgaagctc caggaattag aggttcggat caagaccagc ctccttccat    19380

tgaaggggca agtggtgaac aagcttgcat cttccctgct ctcgtcctcc gccctcggtg    19440

gtctgcagca tgagccttcc tcctccgcat ccatcccgtc gccttcctct tctccttcct    19500

cctcatgcag cacccacacc actcccccca tctcccccgt atcaggggag aagatgacag    19560

tccaggatga tggccggagg gagaggacgc atccgacggc cgccgcgctc atgccctccg    19620

tgcgagtcca acgcctcgac agctcctcca gtggcgccac cacctgctcg gagaactccg    19680

aggaggggcg cggacagctt gacgatatgg agtgcctgag cctgctcatg gaggaagacg    19740

gccagggact ggggaggcca cgatccggca ctgtctttgc ctttctctct gcgtcctctc    19800

cctccggcca tcatcctgga ctgtcatctt ctcccctgat acgggggaga tggggggagt    19860

ggtgtgggtg ctgcatgagg aggaaggaga agaggaaggc gacgggatgg atgcggagga    19920

ggaaggctca tgctgcagac caccgagggc ggaggacgag agcagggaag atgcaagctt    19980

gttcaccact tgccccttca atggaaggag gctggtcttg atccgaacct ctaattcctg    20040

gagcttcggt gcgtcggcgc cggcaagttc ctgaagcacc tttccgatgt agcctaggaa    20100

gaattccact ttcgccgccc gctccattgc ctccacctct ttcgagccct ctctcgacac    20160

taatgctgtc gcttgctgga agcgttcaaa ctcgtgcaag agcagtttaa ggtctgcggt    20220

gagggaatct ttcaagagtt gctgctgctt cgcgtccgcc tcgaacaccg aagacgccac    20280

gggggaggtc agtgatttca gcggagccac cggtgatccg gcactgtctt tgcctttctc    20340

tccacaggtg tccactccca ggttcaatac agctagagag aaaatgatgt gaagaggaag    20400

agataggttt ggaaaaagca tgtacaaagt aattacgatg gagattttgg tgctcttgcc    20460

aggaagaata tatgcttttt gtacactgct gcctctcagg actattttct cgggccttaa    20520

taacacacag tctacttaat taaaaacatc tatcctccag atcaccaggg ccagtgaggc    20580

cggcataaag gacggcaagg aaagaaaaga aagaaagaaa aggacactta tagcatagtt    20640

tgaagttata agtagtcgca atctgtgtgc agccgacaga tgcttttttt ttccgtttgg    20700

caggaggtgt agggatgtcg aagaccagtc cagctagtat ctatcctaca agtcaatcat    20760

gctgcgacaa aaatttctcg cacgaggcct ctcgataaac aaaactttaa aagcacactt    20820

cattgtcatg cagagtaata actcttccgc gtcgatcaat ttatcaatct ctatcatttc    20880

cgcccctttc cttgcataga gcaagaaaag cgacccggat gaggataaca tgtcctgcgc    20940

cagtagtgtg gcattgcctg tctctcattt acacgtactg aaagcataat gcacgcgcat    21000

accaatattt ttcgtgtacg gagatgaaga gacgcgacac gtaagatcac gagaaggcga    21060

gcacggttgc caatggcaga cgcgctagtc tccattatcg cgttgttcgg tagcttgctg    21120

catgtcttca gtggcactat atccactctg cctcgtcttc tacacgaggg ccacatcggt    21180

gcaagttcga aaaatcatat ctcaatcttc agatcctttc cagaaacggt gctcaggcgg    21240

gaaagtgaag gttttctact ctagtggcta ccccaattct ctccgactgt cgcagacggt    21300

ccttcgttgc gcacgcaccg cgcactacct ctgaaattcg acaaccgaag ttcaatttta    21360

catctaactt ctttcccatt ctctcaccaa aagcctagct tacatgttgg agagcgacga    21420

gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga    21480

gttcgagctg gtgggcggcg gagagggcac ccccgagcag ggccgcatga ccaacaagat    21540

gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta    21600

cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat    21660

caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca    21720

cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtgatggg    21780

caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac    21840

cgtggagcac ctgcacccca tgggcgataa cgatctggat ggcagcttca cccgcacctt    21900

cagcctgcgc gacggcggct actacagctc cgtggtggac agccacatgc acttcaagag    21960

cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga    22020

ggaggatcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc    22080

ggatgcagat gccggtgaag aataagggtg ggaaggagtc ggggagggtc ctggcagagc    22140

ggcgtcctca tgatgtgttg gagacctgga gagtcgagag cttcctcgtc acctgattgt    22200

catgtgtgta taggttaagg gggcccactc aaagccataa agacgaacac aaacactaat    22260

ctcaacaaag tctactagca tgccgtctgt ccatctttat ttcctggcgc gcc           22313


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HygR-forward primer

<400>  105
atggggaaga aaccggaact                                                   20


<210>  106
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9 Tracr RNA

<400>  106
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu                                                   80


<210>  107
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA targeting Nannochloropsis acyl-CoA oxidase gene

<400>  107
gacgggggcu guggcgcgcg guuuuagagc uaugcuguuu ug                          42


<210>  108
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA targeting Parachlorella cpSRP54 gene

<400>  108
ggcgugggac auggugcgca guuuuagagc uaugcuguuu ug                          42


<210>  109
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Entire chimeric guide RNA for targeting Nannochloropsis LAR1 gene

<400>  109
ggugugggug cugcaugagg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu                        103


<210>  110
<211>  342
<212>  PRT
<213>  P1 bacteriophage


<220>
<221>  misc_feature
<223>  Cre recombinase

<400>  110

Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val Asp 
1               5                   10                  15      


Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg Asp 
            20                  25                  30          


Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val Cys 
        35                  40                  45              


Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe Pro 
    50                  55                  60                  


Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala Arg 
65                  70                  75                  80  


Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn Met 
                85                  90                  95      


Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala Val 
            100                 105                 110         


Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly Glu 
        115                 120                 125             


Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln Val 
    130                 135                 140                 


Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn Leu 
145                 150                 155                 160 


Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu Ile 
                165                 170                 175     


Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg Met 
            180                 185                 190         


Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly Val 
        195                 200                 205             


Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp Ile 
    210                 215                 220                 


Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys Arg 
225                 230                 235                 240 


Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu Ser 
                245                 250                 255     


Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile Tyr 
            260                 265                 270         


Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly His 
        275                 280                 285             


Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val Ser 
    290                 295                 300                 


Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile Val 
305                 310                 315                 320 


Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val Arg 
                325                 330                 335     


Leu Leu Glu Asp Gly Asp 
            340         


<210>  111
<211>  1511
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic cre gene codon optimized for Nannochloropsis with 
       nuclear localization sequence and intron

<400>  111
atgccgaaaa agaaacgcaa ggtggggtcc aacctgttga cggtgcatca gaacttgcct       60

gccttgcctg tggatgccac atccgatgaa gtgcggaaga acctgatgga catgttccga      120

gacagacaag ccttcagcga gcacacctgg aagatgctgc tgtccgtgtg tagatcttgg      180

gcagcatggt gcaagctcaa taaccggaag tggttcccag ccgaacctga ggacgtgaga      240

gactacctgc tgtacctgca agccagagga ttggcagtga aaaccatcca gcagcacttg      300

ggccagctga acatgttgca tcgacgatcc gggttgccta gacctagcga ctctaatgcc      360

gtgtctctgg tgatgcgccg aatcagaaag gagaacgtgg atgccggaga acgggccaaa      420

caagcattgg cctttgagcg aaccgacttc gaccaagtga gatccttgat ggagaactcc      480

gaccggtgcc aagacatccg gaatctggcg ttcttgggaa tcgcctacaa cacgttgttg      540

agaatagccg agatcgcccg gatccgcgtg aaagacatct ccagaacaga cggaggacgg      600

atgttgatcc atatcggacg gacgaagacc ctggtgtcta cagctggagt ggaaaaggcc      660

ctgtccttgg gagtgacgaa attggtggag cgatggatct ccgtgtctgg agtggccgat      720

gatcccaaca actacctgtt ctgcagagtg cggaagaatg gagtggcagc ccctagtgcc      780

acgtcccaat tgtccacaag agccttagag ggaatcttcg aagccacaca tcgcctgatc      840

tacggcgcca aggacgattc cggacaacgg tatttggcct ggtctggaca ttctgcaaga      900

gtgggagcag cccgagatat ggtaagtgtt tgcaagaggt cgtgcggagg atgaagaggt      960

gcctgagaac gatagatgga aagggtcggg tggccttggt gatggcattc ttttcagagc     1020

tttccgaaca cagtcttgta tctgcagtat taattgatgt atgcagtgtg tatgatccca     1080

cccagtgcct ttatgcagca tgggattgtt aaatagatat gaaagcataa ccggtagaaa     1140

agaaagagag atgagacgct tggtagaacg ccataatcta tgcgttatat gaggagatac     1200

aagcataggc tgtcactcaa tatgtaaatg ggagaagaag cgtatgttac ttgtagatca     1260

gggagacgtg tggataaagc gcgcagcgat ttgtcttccc ctctccgtct cgataccttt     1320

ctgctcggta acaaactgac atggactcta tcttatataa atcacaacgt ttgtaggcgc     1380

gcgctggagt gtccattccc gagatcatgc aagctggagg atggaccaac gtgaacatcg     1440

tgatgaacta catccggaac ctggactccg agacgggagc aatggtgcgg ctgttggaag     1500

atggagatta a                                                          1511


<210>  112
<211>  683
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  Nitrite/Sulfite Reductase Promoter

<400>  112
ctggtgtcgt caacagccag ctgccacaag aaagtgaaca tgcgtctatt tatgacgtca       60

ttcatcaacc accccgtttc caaacaccgt cccacgcgct gttgagagat gattttttga      120

atgccatatg gtgctcaaac atgtgcatcg acgctgtcgc acaagcagga gcgggcttgc      180

ccactcgttc ttgttaacgg cttgattcaa aatccccgcc cggaacaaaa tatgccggag      240

cgatccaacg aagcaaaagt caaccagagc ctctctttcc gtccaacacc cgtgttggtg      300

ccatgttaac aatagattca tgcatggata ggcgaagacg tgagaagtta cggagtttgg      360

gtcatgcttg cgtacatcac tcaacccttt tccccaaaaa aaaatcccgc catgcgattg      420

ccttcgttgc accgcaaaac ggaaattagt tatggcgtca ttgctcaaga ttactgtttt      480

tcgacaaggt gctgcacaac cttggaagaa aactctgcaa atccgtcaat cacatgagtt      540

gtagtttttt tcggcaaggc gggtgagcgt agtgaattat attccttgta aggcaaagcg      600

gatactaatt ttcacgtagt tgccctgacc tcctatgctc ggaaacgccg ccgtactgcc      660

ccacccgaac tcagatcacc agt                                              683


<210>  113
<211>  990
<212>  DNA
<213>  Nannochloropsis gaditana


<220>
<221>  misc_feature
<223>  Nitrite/Sulfite Reductase Terminator

<400>  113
aggcagggtc cccgccaaaa agggtggcga ggacaagaag aagaaacagg aagggggggg       60

gcacgacgga ggacttgtcg agtccatcag ggaggtaggg gcgggaagcc tcgaatctgc      120

tagttggtag ggataaatag agttcaagga ccgaaggagg aggcgccagg atcagcgaaa      180

gcctggatta agagcgagac tccttgcgct gcagtcaagg cgattacagg acccccggtg      240

tctgggtttg gagatgacct cttggaggac ggcttgatgc gggtttttga ggaaggttgt      300

acatttttgt ttgaaatttg caaggaaagc gtcgcgctcc ggcatagagg gataggggga      360

ggaaagggca cttgtgcccg ctccgtctct gtacgggtct ttgaagaaaa gattcgagaa      420

accacccaaa gggcatcaaa tgcgaaacct ccctgaaaaa agtttcgatt ttctttattt      480

gttgaggagg agagggaaga gtggtatcca atgtggggtg tattcacgcc aacaaagcgg      540

ggggagctga cccagaggcc acctgccaca ggctccatcc aaacaagctt tcagggctga      600

ttccagaatt agggttagag taagaatgag ggctacgcca gcagtcatcc tttgcgggcg      660

tcttgagtcg caagaagctc tccaaggaaa gcgaaggcga attttcccca aaaacaaagg      720

cagtggcgag ctccttgtcc ctctttgagc acccctcctc gctaattttc ttactctgat      780

tttttgggga agtgtttctc cttctttcgg agacgtggcc ttatgctcca tcgccttcgc      840

gcaccgactc gaccatgccc acacactctc cgtgcccccc ttccctctgc cacccttccc      900

tctccccccc tcccttcctc cctccctccc tccctccctc cctccctcct cccaggcaca      960

cccctattgt ccacttcgcg ccccaggctc                                       990


<210>  114
<211>  34
<212>  DNA
<213>  P1 bacteriophage


<220>
<221>  misc_feature
<223>  loxP sequence

<400>  114
ataacttcgt ataatgtatg ctatacgaag ttat                                   34


<210>  115
<211>  4341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Floxed HygR-GFP fragment

<400>  115
tccacagccc gaacccctta agctagacga acacagttag cgcggccgca taacttcgta       60

tagcatacat tatacgaagt tatgatgcta gcgtgtttaa gaagtcactt aattaacgta      120

tggtcgacgg ttgctcggat ggggggggcg gggagcgatg gagggaggaa gatcaggtaa      180

ggtctcgaca gactagagaa gcacgagtgc aggtataaga aacagcaaaa aaaagtaatg      240

ggcccaggcc tggagagggt atttgtcttg tttttctttg gccaggaact tgttctcctt      300

tcttcgtttc taggaccccg atccccgctc gcatttctct cttcctcagc cgaagcgcag      360

cggtaaagca tccattttat cccaccgaaa gggcgctccc agccttcgtc gagcggaacc      420

ggggttacag tgcctcactc ctttgcacgc ggtcgggtgc tcggcctacg gttgcccgag      480

tccgcaagca cctcaacaca gccgtctgtc cacaccgcag ccgaccggcg tgcgatttgg      540

gtccgaccca ccgtcccagc cccgctgcgg actatcgcgt cgcagcggcc ctgcgcccac      600

gcggcgtcgt cgaagttgcc gtcgacgaga gactggtaaa gctgatcgag tccgatacgc      660

aacatatagg cgcggagtcg tggggagccg gccagctccg ggtgcctccg ttcaaagtag      720

cgtgtctgct gctccatgca cgccaaccag ggacgccaga agaatatgtt cgccacttcg      780

tattggctat caccaaacat cgcttcggac cagtcgatga cagcagtaat ccgaccattg      840

tctgtaagta cgttattgct gccgaaatcc gcgtgcacca ggtgcctgac ctcagggcaa      900

tcctcggccc acaacatgag ttcgtccagt gcttgggcca cggatgcaga cacggtgtca      960

tccatgactg tctgccaatg atagacgtga ggatcggcaa tggcgcagat gaagtctcgc     1020

caggtcgtgt actgcccgat gccctggggc ccaaaaggtc caaagccgga cgtctgagac     1080

agatctgcgg cagcgatcgc gtccatggcc tcggccacgg gttgcaaaac ggcaggcaat     1140

tcagtttcgg gcagatcttg caacgtcact ccctgggctc ggcgcgagat gcagtacgtg     1200

agagattcgc taaactcccc aatgtccagt acctctggta tggggagagc ggcggaggcg     1260

aaatgacggt agacataccg atccttgtag aacccgtccg cacaactatt aaccctcaac     1320

acgtatcccc gaccccctac gtcaaacgag aacgccctac tctcctctcc ctcgctcagt     1380

tgcatcaagt cggagacaga gtcgaacttc tcaataagga atttctccac ggacgtagcg     1440

gtcagttccg gtttcttccc catcgagctc ggtacccggg gatccatgat tgttgtatta     1500

tgtacctatg tttgtgatga gacaataaat atgagaagag aacgttgcgg ccactttttt     1560

ctccttcctt cgcgtgctca tgttggtggt ttgggaggca gaagatgcat ggagcgccac     1620

acattcggta ggacgaaaca gcctccccca caaagggacc atgggtagct aggatgacgc     1680

acaagcgagt tcccgctctc gaagggaaac ccaggcattt ccttcctctt ttcaagccac     1740

ttgttcacgt gtcaacacaa ttttggacta aaatgcccct cggaactcgg caggcctccc     1800

tctgctccgt tgtcctggtc gccgagaacg cgagaccgtg ccgcatgcca tcgatctgct     1860

cgtctgtact actaatcgtg tgcgtgttcg tgcttgtttc gcacgaaatt gtcctcgttc     1920

ggccctcaca acggtggaaa tcggtgctag aataaagtga ggtggcttat ttcaatggcg     1980

gccgtcatca tgcgggatca actgaagtac ggcgggttct cgagatttca tcgtgctcgt     2040

ccagagcagg tgttttgcct gcagctcttc atgtttaggg gtcatgattt catctgatat     2100

gccgtaagaa aaccaatatt cacttctcaa ttttccatgg aaaggtgaag gcctaggttg     2160

tgtgcgaggc aacgactggg gagggatcgc aacattcttg ctaacctccc ctctatcttg     2220

gccgctgtga atcggcatat ttaccgggct gaattgagaa agtgttttga gggaattaaa     2280

aggtggctgt cttgcaagct tggcttcagt gcctgcttaa ttcgaaccga tccagcttgt     2340

gatgaggcct tcctaagcct ggtagtcaga agcgacatgg cgctataaat ttcgtctcag     2400

ttggagagta gaaaagcatg attcgaacac ggttttcaac tgccaaagat atctccattg     2460

tttccttcaa tctgtacacc tgcacgggcc agtgaggcca ggaaataaag atggacagac     2520

ggcatgctag tagactttgt tgagattagt gtttgtgttc gtctttatgg ctttgagtgg     2580

gcccccttaa cctatacaca catgacaatc aggtgacgag gaagctctcg actctccagg     2640

tctccaacac atcatgagga cgccgctctg ccaggaccct ccccgactcc ttcccaccct     2700

tattcttcac cggcatctgc atccggggtc ttgaaggcgt gctggtactc cacgatgccc     2760

agctcggtgt tgctgtgatc ctcctccacg cggcggaagg cgaacatggg gcccccgttc     2820

tgcaggatgc tggggtggat ggcgctcttg aagtgcatgt ggctgtccac cacggagctg     2880

tagtagccgc cgtcgcgcag gctgaaggtg cgggtgaagc tgccatccag atcgttatcg     2940

cccatggggt gcaggtgctc cacggtggcg ttgctgcgga tgatcttgtc ggtgaagatc     3000

acgctgtcct cggggaagcc ggtgcccatc accttgaagt cgccgatcac gcggccggcc     3060

tcgtagcggt agctgaagct cacgtgcagc acgccgccgt cctcgtactt ctcgatgcgg     3120

gtgttggtgt agccgccgtt gttgatggcg tgcaggaagg ggttctcgta gccgctgggg     3180

taggtgccga agtggtagaa gccgtagccc atcacgtggc tcagcaggta ggggctgaag     3240

gtcagggcgc ctttggtgct cttcatcttg ttggtcatgc ggccctgctc gggggtgccc     3300

tctccgccgc ccaccagctc gaactccacg ccgttcaggg tgccggtgat gcggcactcg     3360

atctccatgg cgggcaggcc gctctcgtcg ctctccaaca tgtaagctag gcttttggtg     3420

agagaatggg aaagaagtta gatgtaaaat tgaacttcgg ttgtcgaatt tcagaggtag     3480

tgcgcggtgc gtgcgcaacg aaggaccgtc tgcgacagtc ggagagaatt ggggtagcca     3540

ctagagtaga aaaccttcac tttcccgcct gagcaccgtt tctggaaagg atctgaagat     3600

tgagatatga tttttcgaac ttgcaccgat gtggccctcg tgtagaagac gaggcagagt     3660

ggatatagtg ccactgaaga catgcagcaa gctaccgaac aacgcgataa tggagactag     3720

cgcgtctgcc attggcaacc gtgctcgcct tctcgtgatc ttacgtgtcg cgtctcttca     3780

tctccgtaca cgaaaaatat tggtatgcgc gtgcattatg ctttcagtac gtgtaaatga     3840

gagacaggca atgccacact actggcgcag gacatgttat cctcatccgg gtcgcttttc     3900

ttgctctatg caaggaaagg ggcggaaatg atagagattg ataaattgat cgacgcggaa     3960

gagttattac tctgcatgac aatgaagtgt gcttttaaag ttttgtttat cgagaggcct     4020

cgtgcgagaa atttttgtcg cagcatgatt gacttgtagg atagatacta gctggactgg     4080

tcttcgacat ccctacacct cctgccaaac ggaaaaaaaa agcatctgtc ggctgcacac     4140

agattgcgac tacttataac ttcaaactat gctataagtg tccttttctt tctttctttt     4200

ctttccttgc cgtcctttat gcccctgcag ggtacgtttt agacggacta ggcagtataa     4260

cttcgtatag catacattat acgaagttat ggcgcgccag gctacgttag ttcagcagct     4320

gagaacgacc acgaacggga a                                               4341


<210>  116
<211>  7536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes fatty acid synthase of Danio rerio, codon optimized for 
       Nannochloropsis

<400>  116
atggaggaca tcgtgattgc cggaatttcc ggaagattgc ctgagagcaa caacttggag       60

gagttttggc agaacctctt caatggcgtg gacatggtga cagaggatga ccgaaggtgg      120

aaacctggat tgtacggatt gcctagacgg aatgggaagt tgaaggagat tgaccggttt      180

gacgccgcat tttttggcgt gcatcctaaa caagcccata cgatggaccc tcaattgcgg      240

ttgatgttgg agatcagtta cgaggcgatt gtggatggag gaattaatcc cgtgagcatg      300

agagggagca aaactggcgt gtacatcgga gtgagcggat ctgaagcagg agaggccttt      360

tctaaagacc ctgaagagtt gttgggatac tccatgacag gatgtcaacg agccatgttc      420

gccaataggc tcagctactt cttcgacttt aacggaccta gcacggcaat cgacacagct      480

tgttcttcta gcttgttggc cttggaaaac gccttcaatg ccatccggca cggacaatgt      540

gatgccgcct tgattggagg agtgaatttg ttgttgaagc ccaatacctc cgtgcaattc      600

atgaagctcg ggatgttgtc ccctgaggga acctgtaagt cctttgatgc ctccggaaat      660

ggatactgtc ggtctgaagc agctgtggct gtgttgttga cgaaaaagtc tatggcaaag      720

cggatctacg ccaccgtgtt gaatgcgggg aacaatacag atggatacaa agagcaagga      780

gtgacctttc ctagcggaga gatgcaacaa cgattggtgc ggtcccttta ccaagaagcc      840

aacatctccc ctgagcaagt ggagtacatt gaggcccatg gaaccggaac caaagtcgga      900

gatcctcaag aagtgaatgg aatcgtgagc gtgttttgcc aatcccctag agatcctttg      960

ttgattggat ccaccaagtc caatatgggg caccctgaac ctgcatctgg attagccgca     1020

ttggccaaag tcgtgttgtc attggagcat ggagtgtggg cacctaacgt gcactttaac     1080

gagcctaacc ctgacatccc tgccttgaca gatggaagat tgtgtgtggt ctccaaacct     1140

acacctgtga gaggaggaat tgtcggaatt aattcctttg gatttggggg atcgaacgtc     1200

cacgtcatct tgtctcccca tgtgagcgac tcttctgaca aaacacctgc cccttctgtg     1260

ccccgattgt tgcaagcttc tggacgaact gaagaggccg ttacagcctt gttttctaat     1320

gcccaacaac accaagagaa ctcctcctac ttgtccctgt tgaatgatgt gtctggtgtg     1380

cctactgctg gaatgcctta ccgaggatac actttgattg gcgcccaagg agaattgacc     1440

gaagtccaac agacccaacc tacgcctcga cctttgtggt acatctgctc cggaatggga     1500

acgcaatggg ctggaatggg acaatctttg atgcaattgt ctgagtttcg agagtccatt     1560

aaaagatccg atatcgccct gaaggacacc ggattgtgcg tgtctcgatt gttgatggag     1620

gccgatgaga gcacctttga agacaccgtc catgcatttg tgggattggc cgccattcaa     1680

gtggcccaaa tcgacatgtt gcggaagatg gggttggagc ctaatggaat tgtgggacat     1740

tctgtgggag aattggcatg tggatatgct gatggatctt tgagccattc cgaagccatt     1800

ttggcagcat actggcgagg aagatgcatt aaagaggcga acttgccccc tggaggtatg     1860

gcagcagtgg gattgacatg ggaagagtgt aaagcgcaat gccctcaagg agtggtgcca     1920

gcttgccata atgcagaaga tacagtgaca atttctggac cccaagactc cgtgtccaaa     1980

tttgtggccc aattgaaaga atctggcgtg tttgcgaaag aggtgcgatc tgctggagtg     2040

gcctttcatt cgtactacat ggcctccatt gcccctgcct tgttgtctgc cttgcaaaaa     2100

gtgatcaagt ccccccgccc tagaacagca cgatggattt ctacatctat tccccaatcc     2160

gactgggaat ctcctcttgc cttgtactct tctgctgagt atcatgtgaa caatcttgtg     2220

tcccctgtcc tcttccaaga ggggttgaat catgtccccg ataatgccgt ggtcgtcgaa     2280

atcgcccctc atgctttgtt gcaagccatc ctcaaacggt ccttgaagcc tacctgttcc     2340

attttgccct tgatgaaacg tggccatgcc aacaacctcg agttcttttt gtcccatgtc     2400

ggaaaagtgt acatgaacgg gatcaacgtg gattccaaca agctttaccc tgccgtgaaa     2460

taccctgtgc ctcgtggaac ccctctgatt tccccttaca tccagtggga tcactcccaa     2520

tcctgggatg tgcctaaagt ggaagacttt cctgccggat ctggcggatc tacatctgca     2580

actgtgtaca atatcgacat gaatcctgaa tcccctgact actacatgat tggacattgt     2640

atcgacggcc gagtgttgta tcctgcaacg ggctacctgg tgttggcttg gagaaccttg     2700

atgcgctctt tgggaacggt gatggaccat acccctgtga cgtttgaaga tgtgaccatc     2760

catcgagcca cgatcttgcc taaaactgga tctgtgcaat tggaagtgcg gttgatgcct     2820

gcaacaaata gatttgaagt gtccgagaat ggcaatttgg ctgtgtccgg gaaggtgtcc     2880

gtgttggaag actctgggtt ggatgcgttt catgccgagc tgaacaagcc cattaccgct     2940

gacaatgaag accctaagct ccgcctgaaa tctggagata tctacaagga gttgcgattg     3000

aggggatacg actacggaaa gacctttcaa ggaattttgg agagcaataa tgccggagac     3060

tccgggaaac tccactggac gggaaattgg gtgacgttct tggacacgat gctgcagatg     3120

atcgtcgtgg gattgcctgg aagaagcttg agactcccta cccgaattcg atctgtgtgt     3180

gtggatccta aattgcatga ggaacgcgtg aatgactacg aaggagacca aaaagccgtg     3240

aatgtttttg tgaatcggtg cctcgacaac atcacagccg gaggagtgca aatctgtggg     3300

ttgcatgcta cagccgcccc tcgaagacaa caacaacaaa caccccctac cttggaggaa     3360

ttcgtctttg tcccctacga agatgccgat tgcttgagaa ccaatgagaa attggccgac     3420

caattgcgac actgtaaagg attggtgcaa cgattgcaac gcaaattggc caaacaaggc     3480

gtgaagatct ccatccctgg cttggaagga gcgtccgaag ggcaattgat tgaagcagag     3540

gccgagaaag ggttgttgag gttgttgtcc gtgttgtgcg gcttggagtt gaatgggaat     3600

ttgagatccg aattggaaca aacggtgcaa aaagagagag actgcttgct gcaagatccc     3660

ctgttgaatg gcttgttgga ttctcaagcg ttgcggcatt gcctggatac cgcgctggaa     3720

aattccacac ctggcaagtt caaggtcttg gaggcccttg ctgctgatgg acgcgtgttt     3780

tctcaagctg tgtctttgct gaatattcaa cctatgttgc gtttggacta cactgcctct     3840

gatatttccg ccgaccaatt gtccgcccaa caatcttctt tggaagagca gggaatctct     3900

accgcccagt gggatcctct tcaaggaccc gtgacaggag gattggatgg agccgatctt     3960

gtggtgtgca attgtgctgt tggatctgcc acgaatcctg cattgttgat tgagaatttg     4020

acatctgccg ctagagaagg aggattcatc ttgttgcaca cgttgttgag aggagatacg     4080

ttgggagaaa cagtggcgtt cctcacgagc cagaataacc ggaagggcct gttgacgcaa     4140

accgagtggg aagagctgtt ccaaaaagcc tccctgaatg tggtgatgtt gcggaagtcc     4200

tattacggaa gtgccttgtt tttgtgtcga agaagccaac aatccagcca aaagcaacct     4260

attcacatct tcgtggaccc taccgactac aaatgggtgg agaccttgaa atccacactc     4320

gccgagtcct ccgatatccc cgtctggttg attgccacca aaggccataa tggagtcgtg     4380

ggaatggtga actgcttgcg tcaagagcct ggaggaaata gaattcgatg cacatttgtc     4440

tccaatttgt ccaaaggagc tgcagtgcct tccttgttgc ctaatgagaa ggtgatgaaa     4500

gccctgttga agaaggacct ggtgatgaac gtccaccgcg acggactgtg gggagtgttt     4560

cgacatcaat tgctgaccca agacctgtcc gaagaattga ccgagcaggc ctacgtgaat     4620

gtgttgacaa gaggagattt gtctagtttg agatggattg cgtccccctt gaggcatttt     4680

gtggcatctt ctcctaatgt gcaattgtgc agagtgtact actcctccct gaacttccgc     4740

gacatcatgc tcgccaccgg aaaattgccc cctgacgcca tccctggaga tgtggcattg     4800

cagcaatgta tgttgggaat ggagttctcc ggaagagatc cttctggaag aagagtgatg     4860

ggattgttgc ctgctaaagg attggccaca tgtgtggatg ccgataaaag atttttgtgg     4920

gatgtgcctt cttcttggac gttggagcaa gccgcctctg tgcctgtcgt ctacgctacc     4980

gcctactact ccttggtggt cagaggaaga ttgagacctg gagaaagtgt gttgattcat     5040

tccggatctg gtggagttgg acaagccgct attgcaattg ccttgagcat gcgctgtaga     5100

gtgtttacaa cagtgggatc taaagagaag aagcaatact tgcaagagcg cttcccccag     5160

ttgacagccg agtccttcgc caattcccgc gattcctcct tcgagcaaca tgtgatgctg     5220

aacacacaag gaaaaggagt ggacctcgtc ttgaattccc tcgccgaaga gaaattgcaa     5280

gcctccttga gatgtcttgc cagacatgga cgatttttgg aaatcggaaa atacgatttg     5340

tccaacaaca cccccctggg catggccctg ttcctgaaga acgtcgcctt tcacgggatc     5400

ttgctcgatg cactgtttga agagggcaat cgtgaatggg aagaggtgtc cgacctgttg     5460

aagaaaggaa tttcctccgg agtggtgcaa cctttgagaa cgaccgtgtt tgagagaaac     5520

caagtggaag aagcatttag atatatggcg cagggaaagc acatcggcaa ggtgttgctg     5580

caagtgcgct ccgaagaatc ctcctcctct ggacctgcag tgtctgcttt gagtattcct     5640

gccatttgcc gaacgttttg tcctgcaagt ttgtcctaca tcatcaccgg aggcttgggc     5700

ggatttggat tggaattggc ccaatggttg acagaaagag gagcaagaaa attggtgttg     5760

acgtctagat ccggaatccg caacggctac caagctaaga gagtccgaga gtggcaagcc     5820

atgggaattc aagtgttggt ctccacatcc gacgtgtcta ccttggatgg aacagaacga     5880

ttgatcacag aggcctgtag gttgggacct gtgggaggaa ttttccattt ggcaatggtg     5940

ttgaaagatg gaatgctcga gaatttgacc cctcaggagt tcatcgaggt gaatcggccc     6000

aagtacgacg ggacgatcaa tttggacagc gtgacccgac aaaaatgccc ccaattgcag     6060

caattcgtcg tgttttcttc cgtgtcttgt ggacgaggga atgccggaca gtccaattac     6120

ggatttgcaa actccacaat ggaaagaatc tgcgaacaaa gacgacagga gaatttgcct     6180

ggattggccg tgcaatgggg agccatcgga gatgtgggag tggtcttgga gacaatggga     6240

ggcaatgatg ccgtgatcgg aggaacatta cctcaaagaa tgtcttcctg cttggaggtg     6300

ttggaccgct ttttgtgtca acaacgacct gtgatgtcct cttttgtgtt ggcagaaaaa     6360

gtggtggtga caaaaggaga gggatccgga caaaaggacc tggtggaagc tgtggcgcac     6420

atcttgggag tgagggacgt gaatagcttg aatgccgacg catcattggc cgatttggga     6480

ttggatagct tgatgggagt ggaagtgaga caaaccttgg agagagacta cgacattgtg     6540

atggctatga gagagatcag acaattgacg atcaacaagt tgcgggagtt gagcaagcaa     6600

tccggcggga aggaggaatc ccctgtgaag aggtctggag cccaagcatt gttggaaagc     6660

gacttgtccc gaatgttggt gaatcctgac ggacctacaa tggcaccctt gaatgaagtc     6720

caatccgcag aaagaccctt gtttttggta catcctatcg aaggatctat tgccgcattt     6780

cgaaccttga cagcgaagct cagcgtgccc tgctacggat tgcaatgtac caaagccgcc     6840

cctttggact ctatccaatc tttggccgcc tactatgtcg aatgtgtgag gcaagtgcag     6900

ttggaaggac cctacagaat tgccggatac tcctttggag cttgtgtcgc ttttgaaatg     6960

tgttcccaat tgcaattggc gaaatgccct gtggagtacc tgttcctgtt cgacggatcc     7020

cactcttacg tcgccgcgta cactcaatct tatcgagcca agttgacccc cggaaaagaa     7080

gctgaagcag aaacagaagc cttgtgtgcc tttatccagc agttcaccgg aatcgagtac     7140

aacaaactct tggagacctt gttgcctttg tctgatttgg aagccagagt ggacaaagca     7200

gtggacctga tcacctcctc ccacaagaac gtgtcccgcg atatgttgca ttttgccgcc     7260

tccacgtttt accacaagtt gaaagccgcc gacagatacg tgcctacatc caaataccac     7320

gggaatgtga ccttgttgag agccaaagca tcttctgagt atggagacgg attgggatct     7380

gactacaaat tgcacgaagt ctgtgacggg aaggtgtccg tgcatgtgat cgagggcgac     7440

catagaacct ttttggaggg agaaggagtg gagtctattt ccagcatcat ccacagcagc     7500

ttgtctgagc ctagagtgtc cacgagagaa ggataa                               7536


<210>  117
<211>  882
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes pantetheine phosphotransferase of Danio rerio, codon 
       optimized for Nannochloropsis

<400>  117
atggatggag tgagatgggc ctttagatgt ggatcttggg tgccttccag atctgagtgg       60

acattggcag caagatgtgt gcaacaagag gagaagttga ggatcggaca gttcgtgttt      120

gccaaagacg ccaaatctgc aatggccgga cgattgttga tcaggaagtt ggtgtgcgag      180

aaaatggggt ttgcctggga cggatttagg ttgcaaagaa cagagcgggg aaaaccttac      240

ttgccccaaa caagttccgc cccttcttgg agctttaatg tgagccatca aggcgattac      300

gcagtgttag cagccgaagc tggaagacaa gtgggaattg acgtcatgaa aacgtccaga      360

cctggaagct cttctgtgca agagttcttc cgcatcatga atcgccaatt taccgacctc      420

gagtggacca atatccgaac cgcaggatct gactgggacc aactgcatat gttttaccgc      480

cattgggcct tgaaagagtc cttcaacaag gcaatcggaa ccggattggg cttcgacttg      540

caacgtgccg aatttcatat ctcccccaac caaatgcgag aaggccaagt gtaccgacaa      600

acaagaatgt acctcgactc cgaagaagaa gaagactgga ccttcgagga gtccttgctc      660

gacaaagatc accatgtggc tgtggccttg ggaaaacctg acatctctat gtccaagaaa      720

gacggaggat cctcctgtga ggcaccacct gcagctttta cagtgttgtc cttttccgac      780

ttggtgtccc aagcaacacc tcttttggac gaagacccta catattggga ggagtttcag      840

aagaagaagg aggccccttc ccgacaatcc gatcaagagt aa                         882


<210>  118
<211>  12447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes Type I fatty acid synthase of Thraustochytrid sp., codon 
       optimized for Nannochloropsis

<400>  118
atgtccgacc agcagcaaca acagcagatc aaggtggagg agacagcccc tgtggcccaa       60

gagcctaaaa cccacttgtt tgtggaccct gaagtgtcct cctgtattgc cgcctttgga      120

ggccaaggat ccgattggtt gggagagttg cgatccttgc acaagaacgg gaaatcctcc      180

gtgcgggagt ttttggaact gggcctgtcc aaattggagg atattgccgc gtccgatgag      240

tggtacgcca accacggggg cttgaatgtg agagcctgga ttgcctccga caaatctgtg      300

ccgagcttcg acctgttgcg ctacgcccct gtgagctttc cgctgatctt catgacgcaa      360

atggccaact acatgcgggt gctggagttg ttgggaacga gccacgagaa agtcgcacaa      420

caaggctggt ttaagggagc cttgggacac agccagggag tcgtcgccgc cgctgtgact      480

gctgctgcat ctactgatcg agaattgaga aacttgtccg tcgccggttt ggagttcatg      540

tcccaaattg gattgggagc ccaaaagagc atgaacttcg agttgtcccg cagatccgcc      600

ggacctgaat cccctatgtt gtctgtgcaa ggcatgtccg aagccacgct cctgaaggcc      660

ttcaaggagg ccacgaagct cgccgtgcag aaagagacca tgatggccaa attttccacg      720

tcctccaagg acgacaaagc cgcccctaat gcctcccaaa gattgggaat tgccttgtgt      780

aatggaaccg acgactacgt ggtgtgtgga gaacctaagg acctgcgcat gttgcggaag      840

gtgatcgtgt ccatgtccgc cgaagtcggg aaagaggccc aagcccgagt gccattcagc      900

aaaagaaaac ctgtgaccca aacgacgttt ctgcgcatga cagcgccatt ccattctgcc      960

ttgaatgccg aagcctttga acaagtggcc gcttgggcag catcttctgc ttttggacag     1020

gaattggccc aacgcacctt gagaatccct gtgtgggaca ccgagaaagg agccgacctc     1080

cgcaagatgg agccttccca agtggtgaac atgttggccc gaaatacctt ggtctcctcc     1140

gccaatttgc tcagcacatt gagagccgcc gaagctgact gtaaagcctc ccacttgatc     1200

tcctttggac ctggatctgt ggccggacac cttatggcca atgcccttgt gggaactgga     1260

atccaggtga tccaggcgaa tgaccctgac tccaagagca aggaaaatgc cgccaccgcg     1320

agcagctcgt cgagccggag cagcgccaaa tctcttgccg ccatcttgac agccgaaaaa     1380

cctgagcgaa tccctgttgc aacaccttgg ggagagaaat tcgcccctaa gattgccgtg     1440

cgtgcgtgtg atggagaacg agtgttgatg accaagtaca cctccaccat cggacgagcc     1500

cctgtgatga tgtccgggat gacgcctacg acgtcgttcc acggaattga cttggtggcc     1560

gcctgtgcaa atgccggata caatgccgaa ttggctgctg gaggattgcc tacacctgac     1620

ctgtttaaga tgaaggtgca ggaactggcc tccaagttga accctggagt gggaatggcc     1680

attaacatgc tgtacctgaa cgcctaccag tggggcttcc agttcccact ggtgtgcgaa     1740

ttggcaaaag ccgggctccc catcgagagc atcacaatcg gagccggagt gcctaccgaa     1800

gaaaaagcca aggacatctt tgacggattg caaggagccg gcatcaactt gatcgggttc     1860

aaacctgggt ccaaacaagc cattagagac gtgctccaac ttgcctcctt gagaccctcc     1920

atgaacatca tgctccagtg gacctccgga cgcggcggcg gacatcacag ctttgaggac     1980

ttccacgagc ccttgttggc cacatacgaa gagatcagac aacacgacaa catcatcctg     2040

gtgatcggaa gcggctttgg agacgcccaa ggagtgatga cataccttga tggatcctgg     2100

tcccaatccc ccaaatttgg acgcttggcc aaaatgcctg tggacggcgt gctcttcggg     2160

tcccggtgta tggtggcgaa agaggcagcc acagcccctg aagtgaaaca attgatcgtc     2220

gatgctgctg gattggaaga cgagttgagc tgggagaagt cctacgacga agtggcagga     2280

ggagtcgtta ctgtgcaatc tgagttggga gaacccattc acaaattggc cacacggggc     2340

atactgttgt ggcgggagtt cgacaagcgg ttcttcagct tgcctcgggg ggagaaacgg     2400

agagatgcca tcttggcagc aaaagacgag attatcgcga agctcaatgc ggacttccag     2460

aaaccctact tcggccgcaa acgagacgga tctgtgtgtg aagtggagga catgacatat     2520

gctgaagtgc tggagcggat ggtgcagctg atgcacatca agaacggggg ggacaaggcc     2580

gggcggttgg cgcctacacg atggattgat cctacatatg cctctagagt gatgctgatg     2640

atgcaacgtt ccgccgcgag attggccaag gacaaaatgg acaaggtggt gccttccaac     2700

aagctgttga tggaagaccc tgacaaagcc atcgccgact tcttggcagc cattcctgcc     2760

ttggaggact ccctgatggc cgacgacgac gtgacctact tcctcgactt gtgcaaagtg     2820

cctacacgtg gaaaacctgt gaacttcgtg cccgtggtgg atgaggactt ggtgttttgg     2880

gtgaagaagg actccctgtg gttttccgag cagttggatg cagtgcctga cagagatcct     2940

ggacgagtgt gcgtgttgca tggacctgtg gctgcccgat actccaccat cgtggatgag     3000

cctattgccg acattatggg gaacatccac aaggacttgg tgcgggacct gaagtgtgac     3060

gaggtgaaag tgaacgtgtt ggcccctgtg gagttgcaac aagcaagcgt gctgaagatc     3120

atctccgaga caccccaatt ggtgagagga cgctcctttg tgcccaaccc catcgccaag     3180

gtgttgaaac gcgaggcctt cgagaaagtg aagtacacat ccgagggagg attggagtcc     3240

atcaacgtgc aagaccctga gcgaggattg accgtggcca ccttgtccca agtcggatcc     3300

gacaacaaat tggcagagtt gcaagtgttc gacaaagaag ccggagccgt gttgaagcag     3360

aagttcacca tcgacctgac cagcctccgc cccatctttc agacagagga ggacaacttg     3420

agcgcaacga agcagttgta cagaaccgcc tgggactgtc aaggcgagtt ccatgccgga     3480

gacacgttca ccgacgaagt ggtggtcaca tccgaaaaca ttgaggcctt taatcgaggg     3540

acgcataccg agtacaatgg atccgcagaa gcccctatcg acatcagcat catggccgga     3600

tggcggcctt tggcccgagc gttgttcgtg gaagaattgc aatccaacct cctgaagctc     3660

gtccatctga ccaacggcat ccggttgccc aaccccaaaa cccgaacacc tgtgaaagcc     3720

ggagaagtga ttagatctga ggtgcgcatt acaggaatca cgatccagcc caaagtgggc     3780

aagaaagtgg cggtgaaggg catcatcacc cgcgcctccg acaaaaatgc cacgcccgaa     3840

atgtgggtcg aaatgaattc cgccttcctc atccgcggag tcgccgagac acctgaagaa     3900

tacgccacga cgttcgaaga gtttcctgcc gaaacccatg tgattgatgt gaaagatgcc     3960

acggtggccg aattggtggc ctctagagcc tggatcaaac tggacaacgg ccgcaaggtg     4020

caagagggag accgagtgac gatttccttg tcccatgtgt ccaatcgctt tgccggaccc     4080

aaccgcttga aggacatcaa ggtgaccgga gacgtgttta tcgagtccac ctccgtgaaa     4140

tcctccaatt ccggatccac ccctttggga tcccctacga attcctccgt ctctggaaca     4200

caatccgacg acttcgtgga cgtggacacc tcctccaagg tgaaagcagg aacagtcgag     4260

tttgcctcct ccgagggcga agagttccag ttgaatcccg tgatgtcgtt cctggaaaag     4320

tactccgagc ctatgcacaa tggccatatc gccgagaatg gcgggtacga gttgatcgcc     4380

gagcctatgg tggtgcctgc acctgctgat tgtacgatgt acgcacgcgg atccagagat     4440

gccaatccta tccatcgcga atccgcattc gccgtgttgg ccgatttgcc tggaggagag     4500

cctatcgtcc atggaatgtg gacagcatgt atggctagag ctagattgga agaaggacct     4560

gccggaggaa atcctgcccg aatcgtctcc tacgaggcct cctttgtgga tatggtgcat     4620

tgcggcgacg aactggtggt gacggctaaa caaacagggg tgaaggacgg actgatgctg     4680

atcaacctga gcgtgaatcg ggcctccgac cgagcattgg tgatgactgc tagagctgaa     4740

ttggaacaac ctacaacggc ctacttgttc acggggcaag gatccgcctc tcccggaatg     4800

ggaatggaca gatatgcagc ttccgcaacg gtgcgcaaag tgtgggatgt ggcagatgag     4860

tacttgagaa accgatacgg gttcagcatc ctccagatcg tgcgggagaa ccccaagtcc     4920

ttgacggtcc actttggagg acctagagga aaggtgattc gcgaaaattt gcgctccctg     4980

cagaccgagg accctacaac cgggaaaaaa atgcccctga tccccgagat ctcctccacg     5040

accaagtcct tcaccttcaa ttcccctacc ggcttgttgt ttgccaccca attctcccaa     5100

cccgccctcg tcttggtgca gaaagccgcc tttgaagaat tgagagaagc cggccttgtc     5160

cctgaaaaag ccttctttgc cggccactct ttgggcgagt atgccgcatt ggccggattt     5220

gccgatgtcc tgtctgtgga ggaccttgtg gaaaccgtgt ttttgagggg aatggtgatg     5280

cagaatgccg tgcctaggga tgccgacgga accagcaatt acgccatggt ggcagcaaat     5340

cctttgagag tcggacgtgg atttacccct gagaccttgg gagaagtcgt ggacttgctg     5400

tgtgagcggg aggacttggg aaagcccttg ctgcagatcg tgaactacaa cgtgagatac     5460

acccagtacg tggtggcagg agagttgctc gcattggacg ccttgggaga agccctgaat     5520

ttggcctttg caaccggaaa taggaatgcc gcggaattgg ccgagaaagg agcacaagca     5580

gccttggcct ctttggcaaa gaggggagga cggaaagagc ccttgaaacg gggaaaagcc     5640

acaattcctt tgcctggaat cgatgtgccc ttccattccc ggaagttgtt gcccggagtg     5700

ggagcgttca gacaactgtt gagccctaga cttgccttga gtacgatgga gcggatctac     5760

caccggttgg tgggcaacta catccctaac gtgacagccg aggtcttgac gttggacaga     5820

tcctacgccg agaaagtgca gaaagtgacc ggaagtgccc ctatgttgga gttgttggcc     5880

gactacgatt ccgccacccc tgccgaaaaa tgccgaacgt tggtgatcga gttgttggca     5940

catcagttcg ccatgcctgt gcgctggatt gagacccaag atctgatgtt tggagcccga     6000

gtgcaacgcg tgatcgagat gggacctgca gccacattga caacaatggc caagcacacg     6060

ttgaaatccg gagcctttgg agacgccgac gagtacaacc ctgagatcat gtggtggaag     6120

aacgaccgag agcgagtgta ctacgagttg gacgacgagg gaccgagctt cgccgccttt     6180

gtggaacaat tgaaggccga gatggagtcc gaggccggag actctgagga gggagaagtg     6240

tccgaagcac ctgcagcacc tgcacctaca cctgctcctg cacctgcacc tgtggcacct     6300

aaacctgcac ctgcacctgt tgctgctcct gcacctgcac cttctggagg agcatctaca     6360

cctgatgcac ctgttgatac aaaacatgtt ttgagagtgt tgttagcctc caagctcaag     6420

aagcccattg gcgaagtccc tgcctctacg tccgtccaaa ccttgtccgc cggccgatct     6480

gctgtccaga acgagatcat gggcgagttg tccgccgagt tcaaaggcgg catccccgac     6540

aatgccggag aaatgccttt gtccgaattg gccggaaatt tgtcctccta caaagaacct     6600

ggagccgtgt ctacaaaact tgtcacccga acattgtctg cagccttgcc tggaggcttt     6660

ggagccaatg ccgccaagga ctacctcacg caacactggg ggttgggaac gggacggaac     6720

ttctccgtgc tgttgcactc ctgcacaatg gcccctgaga aacgcttgaa atctgaggag     6780

gaaggaaaac aatggctcga ctccgtctgc aaagcctacg gtgatgatgt tggagtgtct     6840

ttgtcccctg ccggagctgg aggagctgga ggagcacctt ctggaatgat gatgatgccc     6900

cagatgatgt ccatgggagg acctgccgcc gtgccgccac cggatgcacc tgtctctgca     6960

ttgcatgcta tgagagtgat gttggccaca aaattcgaga aagggttcaa cgagattagc     7020

gattctgcca cagtggcctc tctgtccaac gggaaatccg ccctccagaa cgaggtggcc     7080

ggagatttgg cagccgaatt tggagctgaa ggagacgatt ccgcccagaa gcccctcacc     7140

gaactcgccg ccgcttttca agccggatac tctggacctg gtaaagtttt gtcaagagac     7200

atcaataaag tgttgggaca atgtctcccc ggaggatttg gactgtccgc cgcacgagcc     7260

taccttgcat ctgaccgcct tttgcctgcc ggacgagttg aatccgtgat gatccactcc     7320

ctgaccatgg cccctaagga gcgcatcaag tccgccgaag acgcaaaggc ctggctggat     7380

acagtgtgtg gagcatatgg atcttttgca ggaattgata tccctagagc tggagcagga     7440

ggaggaggag gagccatgat gggatttgcg ggacctggag tgtcttctgc cgaagtgaat     7500

gggttgaaag cgaacctgca gtcgatggtg gagacccagt tggaggccct tcagcggttc     7560

atggagcagg accccttgca tgccgacaga ttgttggatg tggagagaaa attgagagga     7620

gaaacagagg ccaagttgga cgccatccac gccgagttga cagtggactt ctgcgagaga     7680

gtgcaacccc aattcgacga gaaacgtgtg cgagtgtacg actccttctg gaactgggtg     7740

gtgcaagacg cgatgcagat gcacctgcac gtgctgtccc ggttgaatga ggcccggaaa     7800

ggacaatcta caggattacc tgcaggagat gccaatcccc atttcgagga catgtccaag     7860

tggttgttgg gaacgtcgag cagcgaagtg ccccctacag cctggtttcg caatttcttg     7920

tgcaatagag ccacgcccca gttgttgcaa gccgtcaagt tcttcgccaa ctccatgcac     7980

gaggccggac acgtggacta cgcccaagcc attgctttgc ttgccgaaca ggtgcaatgc     8040

tggttgaata atgtgcctgt acatgtcgcc acctttgacc ccgtctcccc caatgtgcga     8100

gtgttggaca atggaacagt cgactacttc gagaccccta gagaaggcgt gcctgacgcc     8160

gtgagatacg tggcagaaat gagccgcggc ctgttctacg tgagaagatc tcctgccaga     8220

gtggccaatc cttcccaagc agtgaatgtg gctggagatg gacaattggc attgcctcct     8280

gctgctgatt ctaccggatt gcaacctgcc gatggagaac ttgcttctgg atggagacga     8340

cctagatccg aggccgaatt ggcccgagag ttgaacaacc ggtccggatc cggccttgaa     8400

gcattggact tggaagccga tgaagcctct gaggagtcta aagaagcctt gcccgaagga     8460

cctaccttgg accgattgag attgacggtg tctagagatg cagcctcttc tggagaagat     8520

gccggaagcc ctgggaaaat ttccacgagc tcgctgaaga acggctacga gtccatccac     8580

gtgtccaagc aggtgccctt cgtccacctc aagtccttgt ccggagtcga caaatctgtg     8640

cgcatcctca atgagcagct tacctccgag tacttttcgt gccttgacga aatcgccaca     8700

tccggagtgt cctttgccgg ccaagtcgcc ttggttacag gagcaggagc aggatccatt     8760

ggaacagagt tggtgaagtc cctgttggag ggaggagcaa ccgtgttgtg tgccatgcga     8820

accgccagat ccgagaatgc attgaccaag gaatacgccc gctttcagaa catctacaag     8880

gagtttgggg ccaaagacag caagctctac ctcgtgccgt gcaactgtgc gtccagccag     8940

gacatgaaga gcatcgtggc ctacacgtac gagcaattgg gattggacgt ggacttcgtc     9000

gtgccctttg ccgcagccgc ccaacaagga aaagatatct cctctattga cgccgcctct     9060

gaagcctctc ataggatgat gatgaccaat gtggtgcgcc tcttgggagc cttgcgtgat     9120

gccaaagcca gccggggaat tgtgacaaga cctgccatgg tgttgatccc ttgctctcct     9180

aatcatggcg agttcgggaa tgacggcctg tacgccgagt ccaaattggg atgtgaagcc     9240

ttgctcaaca agtggtcctc cgagggatgg ggagattatt tgtccttggc cgcctgcgtg     9300

atcggatgga caagaagtgc cctcatggag cacaacaaca ttgtcgcccc cggaatcgaa     9360

gccttgggat gcagaacatt tgcccctgaa gaaacgaact tcaacctggt cggcctcttg     9420

catccccgaa tggtgacctt ggccgccgaa gaacctttgt gggccgattt gacaggaaat     9480

tgggtggtga tccccaacat gaaggacgca gccgacggat tgagaagcga gttgatgacc     9540

aagtcccgca ttgccagagc tgtcgccaca tccaaccaat tggaagagtc caaaaaacct     9600

gagggaggac gggagttgcc gccaccggaa tccgccggac ctttggccgg aacgatgttg     9660

ggaatgacgc cgtttccgac cttgcctagc gaagaagccc gaaagtccct gtctgccttg     9720

gaaggaatgg tggacttgcg gaaagtcgtc gtgatcaccg gatacggaga agtgggacct     9780

tggggaaatg caagaacacg atgggaaatg gagagctacg gagagttctc cctggagggg     9840

gccatcgagt tggcctggat ggtcggattg atcaaaagac atgacggacc tttgcctagc     9900

ggacctcctc gacaacggta tgtgggatgg gtggatgcaa cgagtggaga agcagtggca     9960

gatcatgagg tgaaacggag atacgagaag accctgttgc aatcttgcgg aatccggatt    10020

gtggaacccg ccatcttcga gggctacaac cccgacgcca agcgcttttt gcatagtgtg    10080

gtgcttgacc gagatatgcc cgccatcgag ttggcctctc tggaagaagg gctgcagtac    10140

atgaaggagt tgggagagga gtgctgtgac gtgtttgcca gacctagcga tggacaatac    10200

atgatgcgcg tgaaaaaagg agccgaggtg agcatcgcca aagccctcaa gttcaaccgg    10260

aacgtggccg gacaagtgcc tacaggatgg gatgccagga gattgggatt gcctgccgat    10320

atcgcgaaca gtgtggaccc tgtgacgttg tacacgttgg tgagcaccgt ggaagccttg    10380

atggcagctg gattgtcaga cccttacgaa ttgtaccaat acgtccatgt gagcgaggtg    10440

gggaatacaa gcggaggagg aatgggggga atgaggtcgt tgaagcggat gttccatcag    10500

cggaagctcg acgaggacat ccctagtgac accttggccg agtccttcat caacacgatg    10560

cctgcctggg tgaacatgct gctcgtgtct agctccggac ctatcaaaac acctgtggga    10620

gcatgcgcaa cagcagccga atctttggat attggaatgg agaccatcct cagcggaaaa    10680

gccagagtgg tgatcgccgg aggatacgac gactttgggg aggagggatc ctacgagttc    10740

gcccaaatgg gagccacgaa caacaccgtc ttggatgccg cacgtggaag aacagtgaga    10800

gaatctagcc gacctatgtc gtcgagcagg gcaggatttg tggaatctca tggagccgga    10860

attcaagtgt tgatggatgc cgaattggcc ctggagatgg gagcccctat cttcgccatc    10920

ctggccctga caaataccgc caccgacaaa cagggacgct ccattcctgc acctggacgt    10980

ggaatcttga catctgccag agaaagcacc aaagccggag tgagccctat gctttccttg    11040

gaacgacgaa gacaaggctt ggaaatggaa ttggacgcct tgaagacgct gaatgcccag    11100

aaggagcagt ccgaggggga ggatgccgcc tttcttgaga gactcgtgca gaaacgacga    11160

gcagcagcct tggagacctg gggacaaggg tttttcaaga atgacccctc catcgcccct    11220

ttgagaggag ccttggccgt gtggggattg ggagtggatg atttgggagt tgcatcattt    11280

catggaacgt ctacgaagtt gaacgacacg aacgagtccg gcgtgctgaa caagcagatg    11340

gagcatttgg gacggagcaa gggcaacgtg ctcttcgtgg tggcccagaa gtacttgacg    11400

ggacacccta aaggagccgc ctgtgcgtgg atggtgaacg ggttggtgca gtgtatgttg    11460

gacgcacgag tgcctggaaa tagaaatttg gacaatgtgg atgtgaagtt gcagacgaac    11520

tcctacctcg tgtaccccaa tgagcccgtg caactcccca agatcgaagc cgccctgctg    11580

aagtccttcg gattcggaca agccggagca gaggtcgtga tcgtgcatcc tgaccgcttg    11640

ttggccacct tgtctcctga agccttcgcc aactacatcg aagccagaaa tattagagaa    11700

agacgaacgt tccgaaatgc ccagaacgtg atgtccggat cccgcaacat ggtgatcgtg    11760

aaggagcacc ccccctaccc tgccgagttg gaagaagccg tgtacctcga ccctcttgcc    11820

cgtgcctcct atgatgccaa ggagaatacc tggacgttcc gatccgccgc tggacttaca    11880

tcttctggaa tgcctaaaat tgcgtcgagc caagcgccgt cgtcgccgaa acctgcagca    11940

gcacctgtgt ctgaatctgc ctcccaatcc gcccagatgt ccgccaaaga ccgattgcag    12000

atgacgatgg ccgagcaagc cgccggaatc gcagcaagag ctggaggttc tggtgtggga    12060

gtcggagttg atgtcgaaaa tgtgtccact tttgctgatt acgctggatc caagcaagac    12120

ttcatccaac ggaacttcac cgaagccgag atcgcctact gcaagtccgc cgccgatcct    12180

gccgcctcct ttgctggaag atgggcagcc aaagaagccg tcgtcaaagc cttgtcctcc    12240

attgcccctg attcccgatc cttgtgggcc ggaggtcatg catctttagt tgatatcgaa    12300

gtggtcgcaa atccttctgg agcacctcaa attagattgc acggtcaccc tgagcaagtc    12360

tcccagatgc tcgccgtgaa cgacctgagc gtctccatct cccataccgc cgaagtggca    12420

atcgccaacg ccatcgcccg aaagtga                                        12447


