                         SEQUENCE LISTING

<110>  Intervet International BV
 
<120>  Improved HVT-vectored ND-IBD vaccine

<130>  23888

<160>  1     

<170>  PatentIn version 3.5

<210>  1
<211>  6674
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HVP360 expression cassette with flanking regions


<220>
<221>  gene
<222>  (1)..(399)
<223>  Upstream part of HVT Us2 gene; AF291866, nt 140143-140541

<220>
<221>  promoter
<222>  (630)..(2020)
<223>  murine cytomegalovirus immediate early 1 gene promoter-enhancer

<220>
<221>  gene
<222>  (2052)..(3410)
<223>  IBDV strain 52/70, VP2 gene

<220>
<221>  polyA_signal
<222>  (3441)..(3650)
<223>  SV40 polyA signal + terminator

<220>
<221>  promoter
<222>  (3805)..(4149)
<223>  Human cytomegalovirus immediate early 1 gene promoter core

<220>
<221>  gene
<222>  (4174)..(5835)
<223>  NDV Clone 30 F-gene

<220>
<221>  terminator
<222>  (5815)..(6127)
<223>  hCMV IE gene terminator

<220>
<221>  gene
<222>  (6156)..(6674)
<223>  Downstream part of HVT Us2 gene; AF291866, nt 140541-141059

<400>  1
atctactgtc ttaacgccca tgggaacgga ggcgtcgtcg tcatgtattg gacggcaaca       60

taggcagcaa cacaaattgc gtttaggtgg ggtgcatgtg gactcgatac caagcccctg      120

cagctgggga acgtctggtg gagagccgat aatttgatat acgcacgcca tattactgtc      180

gttgaagtac gccttatctt ctatgttttc aaatttaggt tcccaagtgg acgtgagaag      240

tgtttgtatc tcacatggaa tggcccaagg cattccagcc caggtgcctg gtactttaat      300

ggcaaacaaa cgttttggta gaggtattga ttctattgca gttctgcaga tatctgcagc      360

cccgagtatc cacaggctat acgatacgtt atcggaggca agctgcggcc gctctagaac      420

tagtggatcc cccgggctgc agcccaatgt ggaattcgcc cttgcacatt gttactcctg      480

catcttaaaa atatatcctg tagtaatttt cacagcaatg tcataacatc atctcgctaa      540

agaatgacct gggattggag aagtaatgaa tatttgcaac caatgcattg aataaactaa      600

cattaaacga attcactagt ggatccccca actccgcccg ttttatgact agaaccaata      660

gtttttaatg ccaaatgcac tgaaatcccc taatttgcaa agccaaacgc cccctatgtg      720

agtaatacgg ggacttttta cccaatttcc caagcggaaa gccccctaat acactcatat      780

ggcatatgaa tcagcacggt catgcactct aatggcggcc catagggact ttccacatag      840

ggggcgttca ccatttccca gcataggggt ggtgactcaa tggcctttac ccaagtacat      900

tgggtcaatg ggaggtaagc caatgggttt ttcccattac tggcaagcac actgagtcaa      960

atgggacttt ccactgggtt ttgcccaagt acattgggtc aatgggaggt gagccaatgg     1020

gaaaaaccca ttgctgccaa gtacactgac tcaataggga ctttccaatg ggtttttcca     1080

ttgttggcaa gcatataagg tcaatgtggg tgagtcaata gggactttcc attgtattct     1140

gcccagtaca taaggtcaat agggggtgaa tcaacaggaa agtcccattg gagccaagta     1200

cactgcgtca atagggactt tccattgggt tttgcccagt acataaggtc aataggggat     1260

gagtcaatgg gaaaaaccca ttggagccaa gtacactgac tcaataggga ctttccattg     1320

ggttttgccc agtacataag gtcaataggg ggtgagtcaa caggaaagtc ccattggagc     1380

caagtacatt gagtcaatag ggactttcca atgggttttg cccagtacat aaggtcaatg     1440

ggaggtaagc caatgggttt ttcccattac tggcacgtat actgagtcat tagggacttt     1500

ccaatgggtt ttgcccagta cataaggtca ataggggtga atcaacagga aagtcccatt     1560

ggagccaagt acactgagtc aatagggact ttccattggg ttttgcccag tacaaaaggt     1620

caataggggg tgagtcaatg ggtttttccc attattggca cgtacataag gtcaataggg     1680

gtgagtcatt gggtttttcc agccaattta attaaaacgc catgtacttt cccaccattg     1740

acgtcaatgg gctattgaaa ctaatgcaac gtgaccttta aacggtactt tcccatagct     1800

gattaatggg aaagtaccgt tctcgagcca atacacgtca atgggaagtg aaagggcagc     1860

caaaacgtaa caccgccccg gttttcccct ggaaattcca tattggcacg cattctattg     1920

gctgagctgc gttctacgtg ggtataagag gcgcgaccag cgtcggtacc gtcgcagtct     1980

tcggtctgac caccgtagaa cgcagagctc ctcgctgcag gcggccgctc tagaactcgt     2040

cgatcgcagc gatgacaaac ctgcaagatc aaacccaaca gattgttccg ttcatacgga     2100

gccttctgat gccaacaacc ggaccggcgt ccattccgga cgacaccctg gagaagcaca     2160

ctctcaggtc agagacctcg acctacaatt tgactgtggg ggacacaggg tcagggctaa     2220

ttgtcttttt ccctggattc cctggctcaa ttgtgggtgc tcactacaca ctgcagagca     2280

atgggaacta caagttcgat cagatgctcc tgactgccca gaacctaccg gccagctaca     2340

actactgcag actagtgagt cggagtctca cagtgaggtc aagcacactc cctggtggcg     2400

tttatgcact aaacggcacc ataaacgccg tgaccttcca aggaagcctg agtgaactga     2460

cagatgttag ctacaatggg ttgatgtctg caacagccaa catcaacgac aaaattggga     2520

atgtcctggt aggggaaggg gtcactgtcc tcagcctacc cacatcatat gatcttgggt     2580

atgtgaggct tggtgacccc attcccgcta tagggcttga cccaaaaatg gtagctacat     2640

gcgacagcag tgacaggccc agagtctaca ccataactgc agccgatgat taccaattct     2700

catcacagta ccaaccaggt ggggtaacaa tcacactgtt ctcagccaac attgatgcta     2760

tcacaagcct cagcattggg ggagagctcg tgtttcaaac aagcgtccaa ggccttgtac     2820

tgggcgccac catctacctt ataggctttg atgggactgc ggtaatcacc agagctgtag     2880

ccgcagataa tgggctgacg gccggcaccg acaatcttat gccattcaat cttgtcattc     2940

caaccaatga gataacccag ccaatcacat ccatcaaact ggagatagtg acctccaaaa     3000

gtggtggtca ggcaggggat cagatgtcat ggtcggcaag tgggagccta gcagtgacga     3060

tccatggtgg caactatcca ggggccctcc gtcccgtcac actagtagcc tacgaaagag     3120

tggcaacagg atccgtcgtt acggtcgctg gggtgagtaa cttcgagctg attccaaatc     3180

ctgaactagc aaagaacctg gttacagaat acggccgatt tgacccagga gccatgaact     3240

acacaaaatt gatactgagt gagagggacc gtcttggcat caagaccgtc tggccaacaa     3300

gggagtacac tgattttcgt gagtacttca tggaggtggc cgacctcaac tctcccctga     3360

agattgcagg agcatttggc ttcaaagaca taatccgggc tataaggagg taagcttgat     3420

ctagagcggc cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac     3480

aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt     3540

tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt     3600

tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga caattatttc     3660

atttaataac atatagccca aagacctcta tgaacattta gtttcccgta tactcaacgg     3720

cgcgtgtaca cacaagggcg aattccacag tggatatcaa gcttaattaa gtaccgagct     3780

cgaattggcg cgccaggtca attccctggc attatgccca gtacatgacc ttatgggact     3840

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt     3900

ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc     3960

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc     4020

gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata     4080

taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg     4140

acctccatag aagacaccgg gcgcgccgga tccatgggcc ccagaccttc taccaagaac     4200

ccagtaccta tgatgctgac tgtccgagtc gcgctggtac tgagttgcat ctgtccggca     4260

aactccattg atggcaggcc tcttgcggct gcaggaattg tggttacagg agacaaagcc     4320

gtcaacatat acacctcatc ccagacagga tcaatcatag ttaagctcct cccgaatctg     4380

cccaaggata aggaggcatg tgcgaaagcc cccttggatg catacaacag gacattgacc     4440

actttgctca ccccccttgg tgactctatc cgtaggatac aagagtctgt gactacatct     4500

ggagggggga gacaggggcg ccttataggc gccattattg gcggtgtggc tcttggggtt     4560

gcaactgccg cacaaataac agcggccgca gctctgatac aagccaaaca aaatgctgcc     4620

aacatcctcc gacttaaaga gagcattgcc gcaaccaatg aggctgtgca tgaggtcact     4680

gacggattat cgcaactagc agtggcagtt gggaagatgc agcagtttgt taatgaccaa     4740

tttaataaaa cagctcagga attagactgc atcaaaattg cacagcaagt tggtgtagag     4800

ctcaacctgt acctaaccga attgactaca gtattcggac cacaaatcac ttcacctgct     4860

ttaaacaagc tgactattca ggcactttac aatctagctg gtggaaatat ggattactta     4920

ttgactaagt taggtgtagg gaacaatcaa ctcagctcat taatcggtag cggcttaatc     4980

accggtaacc ctattctata cgactcacag actcaactct tgggtataca ggtaactcta     5040

ccttcagtcg ggaacctaaa taatatgcgt gccacctact tggaaacctt atccgtaagc     5100

acaaccaggg gatttgcctc ggcacttgtc ccaaaagtgg tgacacaggt cggttctgtg     5160

atagaagaac ttgacacctc atactgtata gaaactgact tagatttata ttgtacaaga     5220

atagtaacgt tccctatgtc ccctggtatt tattcctgct tgagcggcaa tacgtcggcc     5280

tgtatgtact caaagaccga aggcgcactt actacaccat acatgactat caaaggttca     5340

gtcatcgcca actgcaagat gacaacatgt agatgtgtaa accccccggg tatcatatcg     5400

caaaactatg gagaagccgt gtctctaata gataaacaat catgcaatgt tttatcctta     5460

ggcgggataa ctttaaggct cagtggggaa ttcgatgtaa cttatcagaa gaatatctca     5520

atacaagatt ctcaagtaat aataacaggc aatcttgata tctcaactga gcttgggaat     5580

gtcaacaact cgatcagtaa tgctttgaat aagttagagg aaagcaacag aaaactagac     5640

aaagtcaatg tcaaactgac tagcacatct gctctcatta cctatatcgt tttgactatc     5700

atatctcttg tttttggtat acttagcccg attctagcat gctacctaat gtacaagcaa     5760

aaggcgcaac aaaagacctt attatggctt gggaataata ctctagatca gatgagagcc     5820

actacaaaaa tgtgaggatc tctcgaggaa ttctagatcc cacgtcacta ttgtatactc     5880

tatattatac tctatgttat actctgtaat cctactcaat aaacgtgtca cgcctgtgaa     5940

accgtactaa gtctcccgtg tcttcttatc accatcaggt gacatcctcg cccaggctgt     6000

caatcatgcc ggtatcgatt ccagtagcac cggccccacg ctgacaaccc actcttgcag     6060

cgttagcagc gcccctctta acaagccgac ccccaccagc gtcgcggtta ctaacactcc     6120

tctccccgac ctgcaactag tgcggccgca gcttgcctcc gattctagca ttacatagcc     6180

ggtcagtaga tcctgccatt cggtagcgca accggctaca tcttcaaaca gtctcacgat     6240

aaatgcatct ctcgttcctg ccaatccgga accgggcata ccactcccgc ctgccgattt     6300

aattctcaca attgggcgat gccggcgggg caaaacgaat gtggatttgg caaaccgaca     6360

caggtctgct gtacggacta atatgggcac acccacatca ttcttcagat gctccatgca     6420

ttgttctatg agaaagatcc atagggtgga ggcagcgtca cgagatcgcc caggcaatcg     6480

atcgcattcg tctagtaaag tgacgagagt tatcatgcac acacccatgc ccacgccttc     6540

cgaataactg gagctgtgga agatcggaaa cgtctttttg actgccggtc tcgtactact     6600

ttcgcacagg tgtatacccg gacgcgtact atatatttta tatcatccaa cgtcccgaaa     6660

ttacatacgt ggcg                                                       6674


