                         SEQUENCE LISTING

<110>  Danisco US, Inc.
       Beck, Zachary Q
       Fujdala, Meredith K
       Hansson, Henrik
       Kaper, Thijs
       Kralj, Slavko
       Liu, Amy D
       Mikkelsen, Nils E
       Sandgren, Mats
 
<120>  ENGINEERED MULTIFUNCTIONAL ENZYMES AND METHODS OF USE

<130>  NB40460WOPCT

<150>  US 62/093630
<151>  2014-12-18

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  2532
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic construct

<400>  1
caaggaagac cgtgagccat tgaaggacag ccggacgcaa tggtgaataa cgcagctctt       60

ctcgccgccc tgtcggctct cctgcccacg gccctggcgc agaacaatca aacatacgcc      120

aactactctg ctcagggcca gcctgatctc taccccgaga cacttgccac gctcacactc      180

tcgttccccg actgcgaaca tggccccctc aagaacaatc tcgtctgtga ctcatcggcc      240

ggctatgtag agcgagccca ggccctcatc tcgctcttca ccctcgagga gctcattctc      300

aacacgcaaa actcgggccc cggcgtgcct cgcctgggtc ttccgaacta ccaagtctgg      360

aatgaggctc tgcacggctt ggaccgcgcc aacttcgcca ccaagggcgg ccagttcgaa      420

tgggcgacct cgttccccat gcccatcctc actacggcgg ccctcaaccg cacattgatc      480

caccagattg ccgacatcat ctcgacccaa gctcgagcat tcagcaacag cggccgttac      540

ggtctcgacg tctatgcgcc aaacgtcaat ggcttccgaa gccccctctg gggccgtggc      600

caggagacgc ccggcgaaga cgcctttttc ctcagctccg cctatactta cgagtacatc      660

acgggcatcc agggtggcgt cgaccctgag cacctcaagg ttgccgccac ggtgaagcac      720

tttgccggat acgacctcga gaactggaac aaccagtccc gtctcggttt cgacgccatc      780

ataactcagc aggacctctc cgaatactac actccccagt tcctcgctgc ggcccgttat      840

gcaaagtcac gcagcttgat gtgcgcatac aactccgtca acggcgtgcc cagctgtgcc      900

aacagcttct tcctgcagac gcttttgcgc gagagctggg gcttccccga atggggatac      960

gtctcgtccg attgcgatgc cgtctacaac gttttcaacc ctcatgacta cgccagcaac     1020

cagtcgtcag ccgccgccag ctcactgcga gccggcaccg atatcgactg cggtcagact     1080

tacccgtggc acctcaacga gtcctttgtg gccggcgaag tctcccgcgg cgagatcgag     1140

cggtccgtca cccgtctgta cgccaacctc gtccgtctcg gatacttcga caagaagaac     1200

cagtaccgct cgctcggttg gaaggatgtc gtcaagactg atgcctggaa catctcgtac     1260

gaggctgctg ttgagggcat cgtcctgctc aagaacgatg gcactctccc tctgtccaag     1320

aaggtgcgca gcattgctct gatcggacca tgggccaatg ccacaaccca aatgcaaggc     1380

aactactatg gccctgcccc atacctcatc agccctctgg aagctgctaa gaaggccggc     1440

tatcacgtca actttgaact cggcacagag atcgccggca acagcaccac tggctttgcc     1500

aaggccattg ctgccgccaa gaagtcggat gccatcatct acctcggtgg aattgacaac     1560

accattgaac aggagggcgc tgaccgcacg gacattgctt ggcccggtaa tcagctggat     1620

ctcatcaagc agctcagcga ggtcggcaaa ccccttgtcg tcctgcaaat gggcggtggt     1680

caggtagact catcctcgct caagagcaac aagaaggtca actccctcgt ctggggcgga     1740

tatcccggcc agtcgggagg cgttgccctc ttcgacattc tctctggcaa gcgtgctcct     1800

gccggccgac tggtcaccac tcagtacccg gctgagtatg ttcaccaatt cccccagaat     1860

gacatgaacc tccgacccga tggaaagtca aaccctggac agacttacat ctggtacacc     1920

ggcaaacccg tctacgagtt tggcagtggt ctcttctaca ccaccttcaa ggagactctc     1980

gccagccacc ccaagagcct caagttcaac acctcatcga tcctctctgc tcctcacccc     2040

ggatacactt acagcgagca gattcccgtc ttcaccttcg aggccaacat caagaactcg     2100

ggcaagacgg agtccccata tacggccatg ctgtttgttc gcacaagcaa cgctggccca     2160

gccccgtacc cgaacaagtg gctcgtcgga ttcgaccgac ttgccgacat caagcctggt     2220

cactcttcca agctcagcat ccccatccct gtcagtgctc tcgcccgtgt tgattctcac     2280

ggaaaccgga ttgtataccc cggcaagtat gagctagcct tgaacaccga cgagtctgtg     2340

aagcttgagt ttgagttggt gggagaagag gtaacgattg agaactggcc gttggaggag     2400

caacagatca aggatgctac acctgacgca taagggtttt aatgatgttg ttatgacaaa     2460

cgggtagagt agttaatgat ggaataggaa gaggccatag ttttctgttt gcaaaccatt     2520

tttgccattg cg                                                         2532


<210>  2
<211>  797
<212>  PRT
<213>  Trichoderma reesei

<400>  2

Met Val Asn Asn Ala Ala Leu Leu Ala Ala Leu Ser Ala Leu Leu Pro 
1               5                   10                  15      


Thr Ala Leu Ala Gln Asn Asn Gln Thr Tyr Ala Asn Tyr Ser Ala Gln 
            20                  25                  30          


Gly Gln Pro Asp Leu Tyr Pro Glu Thr Leu Ala Thr Leu Thr Leu Ser 
        35                  40                  45              


Phe Pro Asp Cys Glu His Gly Pro Leu Lys Asn Asn Leu Val Cys Asp 
    50                  55                  60                  


Ser Ser Ala Gly Tyr Val Glu Arg Ala Gln Ala Leu Ile Ser Leu Phe 
65                  70                  75                  80  


Thr Leu Glu Glu Leu Ile Leu Asn Thr Gln Asn Ser Gly Pro Gly Val 
                85                  90                  95      


Pro Arg Leu Gly Leu Pro Asn Tyr Gln Val Trp Asn Glu Ala Leu His 
            100                 105                 110         


Gly Leu Asp Arg Ala Asn Phe Ala Thr Lys Gly Gly Gln Phe Glu Trp 
        115                 120                 125             


Ala Thr Ser Phe Pro Met Pro Ile Leu Thr Thr Ala Ala Leu Asn Arg 
    130                 135                 140                 


Thr Leu Ile His Gln Ile Ala Asp Ile Ile Ser Thr Gln Ala Arg Ala 
145                 150                 155                 160 


Phe Ser Asn Ser Gly Arg Tyr Gly Leu Asp Val Tyr Ala Pro Asn Val 
                165                 170                 175     


Asn Gly Phe Arg Ser Pro Leu Trp Gly Arg Gly Gln Glu Thr Pro Gly 
            180                 185                 190         


Glu Asp Ala Phe Phe Leu Ser Ser Ala Tyr Thr Tyr Glu Tyr Ile Thr 
        195                 200                 205             


Gly Ile Gln Gly Gly Val Asp Pro Glu His Leu Lys Val Ala Ala Thr 
    210                 215                 220                 


Val Lys His Phe Ala Gly Tyr Asp Leu Glu Asn Trp Asn Asn Gln Ser 
225                 230                 235                 240 


Arg Leu Gly Phe Asp Ala Ile Ile Thr Gln Gln Asp Leu Ser Glu Tyr 
                245                 250                 255     


Tyr Thr Pro Gln Phe Leu Ala Ala Ala Arg Tyr Ala Lys Ser Arg Ser 
            260                 265                 270         


Leu Met Cys Ala Tyr Asn Ser Val Asn Gly Val Pro Ser Cys Ala Asn 
        275                 280                 285             


Ser Phe Phe Leu Gln Thr Leu Leu Arg Glu Ser Trp Gly Phe Pro Glu 
    290                 295                 300                 


Trp Gly Tyr Val Ser Ser Asp Cys Asp Ala Val Tyr Asn Val Phe Asn 
305                 310                 315                 320 


Pro His Asp Tyr Ala Ser Asn Gln Ser Ser Ala Ala Ala Ser Ser Leu 
                325                 330                 335     


Arg Ala Gly Thr Asp Ile Asp Cys Gly Gln Thr Tyr Pro Trp His Leu 
            340                 345                 350         


Asn Glu Ser Phe Val Ala Gly Glu Val Ser Arg Gly Glu Ile Glu Arg 
        355                 360                 365             


Ser Val Thr Arg Leu Tyr Ala Asn Leu Val Arg Leu Gly Tyr Phe Asp 
    370                 375                 380                 


Lys Lys Asn Gln Tyr Arg Ser Leu Gly Trp Lys Asp Val Val Lys Thr 
385                 390                 395                 400 


Asp Ala Trp Asn Ile Ser Tyr Glu Ala Ala Val Glu Gly Ile Val Leu 
                405                 410                 415     


Leu Lys Asn Asp Gly Thr Leu Pro Leu Ser Lys Lys Val Arg Ser Ile 
            420                 425                 430         


Ala Leu Ile Gly Pro Trp Ala Asn Ala Thr Thr Gln Met Gln Gly Asn 
        435                 440                 445             


Tyr Tyr Gly Pro Ala Pro Tyr Leu Ile Ser Pro Leu Glu Ala Ala Lys 
    450                 455                 460                 


Lys Ala Gly Tyr His Val Asn Phe Glu Leu Gly Thr Glu Ile Ala Gly 
465                 470                 475                 480 


Asn Ser Thr Thr Gly Phe Ala Lys Ala Ile Ala Ala Ala Lys Lys Ser 
                485                 490                 495     


Asp Ala Ile Ile Tyr Leu Gly Gly Ile Asp Asn Thr Ile Glu Gln Glu 
            500                 505                 510         


Gly Ala Asp Arg Thr Asp Ile Ala Trp Pro Gly Asn Gln Leu Asp Leu 
        515                 520                 525             


Ile Lys Gln Leu Ser Glu Val Gly Lys Pro Leu Val Val Leu Gln Met 
    530                 535                 540                 


Gly Gly Gly Gln Val Asp Ser Ser Ser Leu Lys Ser Asn Lys Lys Val 
545                 550                 555                 560 


Asn Ser Leu Val Trp Gly Gly Tyr Pro Gly Gln Ser Gly Gly Val Ala 
                565                 570                 575     


Leu Phe Asp Ile Leu Ser Gly Lys Arg Ala Pro Ala Gly Arg Leu Val 
            580                 585                 590         


Thr Thr Gln Tyr Pro Ala Glu Tyr Val His Gln Phe Pro Gln Asn Asp 
        595                 600                 605             


Met Asn Leu Arg Pro Asp Gly Lys Ser Asn Pro Gly Gln Thr Tyr Ile 
    610                 615                 620                 


Trp Tyr Thr Gly Lys Pro Val Tyr Glu Phe Gly Ser Gly Leu Phe Tyr 
625                 630                 635                 640 


Thr Thr Phe Lys Glu Thr Leu Ala Ser His Pro Lys Ser Leu Lys Phe 
                645                 650                 655     


Asn Thr Ser Ser Ile Leu Ser Ala Pro His Pro Gly Tyr Thr Tyr Ser 
            660                 665                 670         


Glu Gln Ile Pro Val Phe Thr Phe Glu Ala Asn Ile Lys Asn Ser Gly 
        675                 680                 685             


Lys Thr Glu Ser Pro Tyr Thr Ala Met Leu Phe Val Arg Thr Ser Asn 
    690                 695                 700                 


Ala Gly Pro Ala Pro Tyr Pro Asn Lys Trp Leu Val Gly Phe Asp Arg 
705                 710                 715                 720 


Leu Ala Asp Ile Lys Pro Gly His Ser Ser Lys Leu Ser Ile Pro Ile 
                725                 730                 735     


Pro Val Ser Ala Leu Ala Arg Val Asp Ser His Gly Asn Arg Ile Val 
            740                 745                 750         


Tyr Pro Gly Lys Tyr Glu Leu Ala Leu Asn Thr Asp Glu Ser Val Lys 
        755                 760                 765             


Leu Glu Phe Glu Leu Val Gly Glu Glu Val Thr Ile Glu Asn Trp Pro 
    770                 775                 780                 


Leu Glu Glu Gln Gln Ile Lys Asp Ala Thr Pro Asp Ala 
785                 790                 795         


<210>  3
<211>  777
<212>  PRT
<213>  Trichoderma reesei

<400>  3

Gln Asn Asn Gln Thr Tyr Ala Asn Tyr Ser Ala Gln Gly Gln Pro Asp 
1               5                   10                  15      


Leu Tyr Pro Glu Thr Leu Ala Thr Leu Thr Leu Ser Phe Pro Asp Cys 
            20                  25                  30          


Glu His Gly Pro Leu Lys Asn Asn Leu Val Cys Asp Ser Ser Ala Gly 
        35                  40                  45              


Tyr Val Glu Arg Ala Gln Ala Leu Ile Ser Leu Phe Thr Leu Glu Glu 
    50                  55                  60                  


Leu Ile Leu Asn Thr Gln Asn Ser Gly Pro Gly Val Pro Arg Leu Gly 
65                  70                  75                  80  


Leu Pro Asn Tyr Gln Val Trp Asn Glu Ala Leu His Gly Leu Asp Arg 
                85                  90                  95      


Ala Asn Phe Ala Thr Lys Gly Gly Gln Phe Glu Trp Ala Thr Ser Phe 
            100                 105                 110         


Pro Met Pro Ile Leu Thr Thr Ala Ala Leu Asn Arg Thr Leu Ile His 
        115                 120                 125             


Gln Ile Ala Asp Ile Ile Ser Thr Gln Ala Arg Ala Phe Ser Asn Ser 
    130                 135                 140                 


Gly Arg Tyr Gly Leu Asp Val Tyr Ala Pro Asn Val Asn Gly Phe Arg 
145                 150                 155                 160 


Ser Pro Leu Trp Gly Arg Gly Gln Glu Thr Pro Gly Glu Asp Ala Phe 
                165                 170                 175     


Phe Leu Ser Ser Ala Tyr Thr Tyr Glu Tyr Ile Thr Gly Ile Gln Gly 
            180                 185                 190         


Gly Val Asp Pro Glu His Leu Lys Val Ala Ala Thr Val Lys His Phe 
        195                 200                 205             


Ala Gly Tyr Asp Leu Glu Asn Trp Asn Asn Gln Ser Arg Leu Gly Phe 
    210                 215                 220                 


Asp Ala Ile Ile Thr Gln Gln Asp Leu Ser Glu Tyr Tyr Thr Pro Gln 
225                 230                 235                 240 


Phe Leu Ala Ala Ala Arg Tyr Ala Lys Ser Arg Ser Leu Met Cys Ala 
                245                 250                 255     


Tyr Asn Ser Val Asn Gly Val Pro Ser Cys Ala Asn Ser Phe Phe Leu 
            260                 265                 270         


Gln Thr Leu Leu Arg Glu Ser Trp Gly Phe Pro Glu Trp Gly Tyr Val 
        275                 280                 285             


Ser Ser Asp Cys Asp Ala Val Tyr Asn Val Phe Asn Pro His Asp Tyr 
    290                 295                 300                 


Ala Ser Asn Gln Ser Ser Ala Ala Ala Ser Ser Leu Arg Ala Gly Thr 
305                 310                 315                 320 


Asp Ile Asp Cys Gly Gln Thr Tyr Pro Trp His Leu Asn Glu Ser Phe 
                325                 330                 335     


Val Ala Gly Glu Val Ser Arg Gly Glu Ile Glu Arg Ser Val Thr Arg 
            340                 345                 350         


Leu Tyr Ala Asn Leu Val Arg Leu Gly Tyr Phe Asp Lys Lys Asn Gln 
        355                 360                 365             


Tyr Arg Ser Leu Gly Trp Lys Asp Val Val Lys Thr Asp Ala Trp Asn 
    370                 375                 380                 


Ile Ser Tyr Glu Ala Ala Val Glu Gly Ile Val Leu Leu Lys Asn Asp 
385                 390                 395                 400 


Gly Thr Leu Pro Leu Ser Lys Lys Val Arg Ser Ile Ala Leu Ile Gly 
                405                 410                 415     


Pro Trp Ala Asn Ala Thr Thr Gln Met Gln Gly Asn Tyr Tyr Gly Pro 
            420                 425                 430         


Ala Pro Tyr Leu Ile Ser Pro Leu Glu Ala Ala Lys Lys Ala Gly Tyr 
        435                 440                 445             


His Val Asn Phe Glu Leu Gly Thr Glu Ile Ala Gly Asn Ser Thr Thr 
    450                 455                 460                 


Gly Phe Ala Lys Ala Ile Ala Ala Ala Lys Lys Ser Asp Ala Ile Ile 
465                 470                 475                 480 


Tyr Leu Gly Gly Ile Asp Asn Thr Ile Glu Gln Glu Gly Ala Asp Arg 
                485                 490                 495     


Thr Asp Ile Ala Trp Pro Gly Asn Gln Leu Asp Leu Ile Lys Gln Leu 
            500                 505                 510         


Ser Glu Val Gly Lys Pro Leu Val Val Leu Gln Met Gly Gly Gly Gln 
        515                 520                 525             


Val Asp Ser Ser Ser Leu Lys Ser Asn Lys Lys Val Asn Ser Leu Val 
    530                 535                 540                 


Trp Gly Gly Tyr Pro Gly Gln Ser Gly Gly Val Ala Leu Phe Asp Ile 
545                 550                 555                 560 


Leu Ser Gly Lys Arg Ala Pro Ala Gly Arg Leu Val Thr Thr Gln Tyr 
                565                 570                 575     


Pro Ala Glu Tyr Val His Gln Phe Pro Gln Asn Asp Met Asn Leu Arg 
            580                 585                 590         


Pro Asp Gly Lys Ser Asn Pro Gly Gln Thr Tyr Ile Trp Tyr Thr Gly 
        595                 600                 605             


Lys Pro Val Tyr Glu Phe Gly Ser Gly Leu Phe Tyr Thr Thr Phe Lys 
    610                 615                 620                 


Glu Thr Leu Ala Ser His Pro Lys Ser Leu Lys Phe Asn Thr Ser Ser 
625                 630                 635                 640 


Ile Leu Ser Ala Pro His Pro Gly Tyr Thr Tyr Ser Glu Gln Ile Pro 
                645                 650                 655     


Val Phe Thr Phe Glu Ala Asn Ile Lys Asn Ser Gly Lys Thr Glu Ser 
            660                 665                 670         


Pro Tyr Thr Ala Met Leu Phe Val Arg Thr Ser Asn Ala Gly Pro Ala 
        675                 680                 685             


Pro Tyr Pro Asn Lys Trp Leu Val Gly Phe Asp Arg Leu Ala Asp Ile 
    690                 695                 700                 


Lys Pro Gly His Ser Ser Lys Leu Ser Ile Pro Ile Pro Val Ser Ala 
705                 710                 715                 720 


Leu Ala Arg Val Asp Ser His Gly Asn Arg Ile Val Tyr Pro Gly Lys 
                725                 730                 735     


Tyr Glu Leu Ala Leu Asn Thr Asp Glu Ser Val Lys Leu Glu Phe Glu 
            740                 745                 750         


Leu Val Gly Glu Glu Val Thr Ile Glu Asn Trp Pro Leu Glu Glu Gln 
        755                 760                 765             


Gln Ile Lys Asp Ala Thr Pro Asp Ala 
    770                 775         


<210>  4
<211>  744
<212>  PRT
<213>  Trichoderma reesei

<400>  4

Met Arg Tyr Arg Thr Ala Ala Ala Leu Ala Leu Ala Thr Gly Pro Phe 
1               5                   10                  15      


Ala Arg Ala Asp Ser His Ser Thr Ser Gly Ala Ser Ala Glu Ala Val 
            20                  25                  30          


Val Pro Pro Ala Gly Thr Pro Trp Gly Thr Ala Tyr Asp Lys Ala Lys 
        35                  40                  45              


Ala Ala Leu Ala Lys Leu Asn Leu Gln Asp Lys Val Gly Ile Val Ser 
    50                  55                  60                  


Gly Val Gly Trp Asn Gly Gly Pro Cys Val Gly Asn Thr Ser Pro Ala 
65                  70                  75                  80  


Ser Lys Ile Ser Tyr Pro Ser Leu Cys Leu Gln Asp Gly Pro Leu Gly 
                85                  90                  95      


Val Arg Tyr Ser Thr Gly Ser Thr Ala Phe Thr Pro Gly Val Gln Ala 
            100                 105                 110         


Ala Ser Thr Trp Asp Val Asn Leu Ile Arg Glu Arg Gly Gln Phe Ile 
        115                 120                 125             


Gly Glu Glu Val Lys Ala Ser Gly Ile His Val Ile Leu Gly Pro Val 
    130                 135                 140                 


Ala Gly Pro Leu Gly Lys Thr Pro Gln Gly Gly Arg Asn Trp Glu Gly 
145                 150                 155                 160 


Phe Gly Val Asp Pro Tyr Leu Thr Gly Ile Ala Met Gly Gln Thr Ile 
                165                 170                 175     


Asn Gly Ile Gln Ser Val Gly Val Gln Ala Thr Ala Lys His Tyr Ile 
            180                 185                 190         


Leu Asn Glu Gln Glu Leu Asn Arg Glu Thr Ile Ser Ser Asn Pro Asp 
        195                 200                 205             


Asp Arg Thr Leu His Glu Leu Tyr Thr Trp Pro Phe Ala Asp Ala Val 
    210                 215                 220                 


Gln Ala Asn Val Ala Ser Val Met Cys Ser Tyr Asn Lys Val Asn Thr 
225                 230                 235                 240 


Thr Trp Ala Cys Glu Asp Gln Tyr Thr Leu Gln Thr Val Leu Lys Asp 
                245                 250                 255     


Gln Leu Gly Phe Pro Gly Tyr Val Met Thr Asp Trp Asn Ala Gln His 
            260                 265                 270         


Thr Thr Val Gln Ser Ala Asn Ser Gly Leu Asp Met Ser Met Pro Gly 
        275                 280                 285             


Thr Asp Phe Asn Gly Asn Asn Arg Leu Trp Gly Pro Ala Leu Thr Asn 
    290                 295                 300                 


Ala Val Asn Ser Asn Gln Val Pro Thr Ser Arg Val Asp Asp Met Val 
305                 310                 315                 320 


Thr Arg Ile Leu Ala Ala Trp Tyr Leu Thr Gly Gln Asp Gln Ala Gly 
                325                 330                 335     


Tyr Pro Ser Phe Asn Ile Ser Arg Asn Val Gln Gly Asn His Lys Thr 
            340                 345                 350         


Asn Val Arg Ala Ile Ala Arg Asp Gly Ile Val Leu Leu Lys Asn Asp 
        355                 360                 365             


Ala Asn Ile Leu Pro Leu Lys Lys Pro Ala Ser Ile Ala Val Val Gly 
    370                 375                 380                 


Ser Ala Ala Ile Ile Gly Asn His Ala Arg Asn Ser Pro Ser Cys Asn 
385                 390                 395                 400 


Asp Lys Gly Cys Asp Asp Gly Ala Leu Gly Met Gly Trp Gly Ser Gly 
                405                 410                 415     


Ala Val Asn Tyr Pro Tyr Phe Val Ala Pro Tyr Asp Ala Ile Asn Thr 
            420                 425                 430         


Arg Ala Ser Ser Gln Gly Thr Gln Val Thr Leu Ser Asn Thr Asp Asn 
        435                 440                 445             


Thr Ser Ser Gly Ala Ser Ala Ala Arg Gly Lys Asp Val Ala Ile Val 
    450                 455                 460                 


Phe Ile Thr Ala Asp Ser Gly Glu Gly Tyr Ile Thr Val Glu Gly Asn 
465                 470                 475                 480 


Ala Gly Asp Arg Asn Asn Leu Asp Pro Trp His Asn Gly Asn Ala Leu 
                485                 490                 495     


Val Gln Ala Val Ala Gly Ala Asn Ser Asn Val Ile Val Val Val His 
            500                 505                 510         


Ser Val Gly Ala Ile Ile Leu Glu Gln Ile Leu Ala Leu Pro Gln Val 
        515                 520                 525             


Lys Ala Val Val Trp Ala Gly Leu Pro Ser Gln Glu Ser Gly Asn Ala 
    530                 535                 540                 


Leu Val Asp Val Leu Trp Gly Asp Val Ser Pro Ser Gly Lys Leu Val 
545                 550                 555                 560 


Tyr Thr Ile Ala Lys Ser Pro Asn Asp Tyr Asn Thr Arg Ile Val Ser 
                565                 570                 575     


Gly Gly Ser Asp Ser Phe Ser Glu Gly Leu Phe Ile Asp Tyr Lys His 
            580                 585                 590         


Phe Asp Asp Ala Asn Ile Thr Pro Arg Tyr Glu Phe Gly Tyr Gly Leu 
        595                 600                 605             


Ser Tyr Thr Lys Phe Asn Tyr Ser Arg Leu Ser Val Leu Ser Thr Ala 
    610                 615                 620                 


Lys Ser Gly Pro Ala Thr Gly Ala Val Val Pro Gly Gly Pro Ser Asp 
625                 630                 635                 640 


Leu Phe Gln Asn Val Ala Thr Val Thr Val Asp Ile Ala Asn Ser Gly 
                645                 650                 655     


Gln Val Thr Gly Ala Glu Val Ala Gln Leu Tyr Ile Thr Tyr Pro Ser 
            660                 665                 670         


Ser Ala Pro Arg Thr Pro Pro Lys Gln Leu Arg Gly Phe Ala Lys Leu 
        675                 680                 685             


Asn Leu Thr Pro Gly Gln Ser Gly Thr Ala Thr Phe Asn Ile Arg Arg 
    690                 695                 700                 


Arg Asp Leu Ser Tyr Trp Asp Thr Ala Ser Gln Lys Trp Val Val Pro 
705                 710                 715                 720 


Ser Gly Ser Phe Gly Ile Ser Val Gly Ala Ser Ser Arg Asp Ile Arg 
                725                 730                 735     


Leu Thr Ser Thr Leu Ser Val Ala 
            740                 


<210>  5
<211>  713
<212>  PRT
<213>  Trichoderma reesei

<400>  5

Val Val Pro Pro Ala Gly Thr Pro Trp Gly Thr Ala Tyr Asp Lys Ala 
1               5                   10                  15      


Lys Ala Ala Leu Ala Lys Leu Asn Leu Gln Asp Lys Val Gly Ile Val 
            20                  25                  30          


Ser Gly Val Gly Trp Asn Gly Gly Pro Cys Val Gly Asn Thr Ser Pro 
        35                  40                  45              


Ala Ser Lys Ile Ser Tyr Pro Ser Leu Cys Leu Gln Asp Gly Pro Leu 
    50                  55                  60                  


Gly Val Arg Tyr Ser Thr Gly Ser Thr Ala Phe Thr Pro Gly Val Gln 
65                  70                  75                  80  


Ala Ala Ser Thr Trp Asp Val Asn Leu Ile Arg Glu Arg Gly Gln Phe 
                85                  90                  95      


Ile Gly Glu Glu Val Lys Ala Ser Gly Ile His Val Ile Leu Gly Pro 
            100                 105                 110         


Val Ala Gly Pro Leu Gly Lys Thr Pro Gln Gly Gly Arg Asn Trp Glu 
        115                 120                 125             


Gly Phe Gly Val Asp Pro Tyr Leu Thr Gly Ile Ala Met Gly Gln Thr 
    130                 135                 140                 


Ile Asn Gly Ile Gln Ser Val Gly Val Gln Ala Thr Ala Lys His Tyr 
145                 150                 155                 160 


Ile Leu Asn Glu Gln Glu Leu Asn Arg Glu Thr Ile Ser Ser Asn Pro 
                165                 170                 175     


Asp Asp Arg Thr Leu His Glu Leu Tyr Thr Trp Pro Phe Ala Asp Ala 
            180                 185                 190         


Val Gln Ala Asn Val Ala Ser Val Met Cys Ser Tyr Asn Lys Val Asn 
        195                 200                 205             


Thr Thr Trp Ala Cys Glu Asp Gln Tyr Thr Leu Gln Thr Val Leu Lys 
    210                 215                 220                 


Asp Gln Leu Gly Phe Pro Gly Tyr Val Met Thr Asp Trp Asn Ala Gln 
225                 230                 235                 240 


His Thr Thr Val Gln Ser Ala Asn Ser Gly Leu Asp Met Ser Met Pro 
                245                 250                 255     


Gly Thr Asp Phe Asn Gly Asn Asn Arg Leu Trp Gly Pro Ala Leu Thr 
            260                 265                 270         


Asn Ala Val Asn Ser Asn Gln Val Pro Thr Ser Arg Val Asp Asp Met 
        275                 280                 285             


Val Thr Arg Ile Leu Ala Ala Trp Tyr Leu Thr Gly Gln Asp Gln Ala 
    290                 295                 300                 


Gly Tyr Pro Ser Phe Asn Ile Ser Arg Asn Val Gln Gly Asn His Lys 
305                 310                 315                 320 


Thr Asn Val Arg Ala Ile Ala Arg Asp Gly Ile Val Leu Leu Lys Asn 
                325                 330                 335     


Asp Ala Asn Ile Leu Pro Leu Lys Lys Pro Ala Ser Ile Ala Val Val 
            340                 345                 350         


Gly Ser Ala Ala Ile Ile Gly Asn His Ala Arg Asn Ser Pro Ser Cys 
        355                 360                 365             


Asn Asp Lys Gly Cys Asp Asp Gly Ala Leu Gly Met Gly Trp Gly Ser 
    370                 375                 380                 


Gly Ala Val Asn Tyr Pro Tyr Phe Val Ala Pro Tyr Asp Ala Ile Asn 
385                 390                 395                 400 


Thr Arg Ala Ser Ser Gln Gly Thr Gln Val Thr Leu Ser Asn Thr Asp 
                405                 410                 415     


Asn Thr Ser Ser Gly Ala Ser Ala Ala Arg Gly Lys Asp Val Ala Ile 
            420                 425                 430         


Val Phe Ile Thr Ala Asp Ser Gly Glu Gly Tyr Ile Thr Val Glu Gly 
        435                 440                 445             


Asn Ala Gly Asp Arg Asn Asn Leu Asp Pro Trp His Asn Gly Asn Ala 
    450                 455                 460                 


Leu Val Gln Ala Val Ala Gly Ala Asn Ser Asn Val Ile Val Val Val 
465                 470                 475                 480 


His Ser Val Gly Ala Ile Ile Leu Glu Gln Ile Leu Ala Leu Pro Gln 
                485                 490                 495     


Val Lys Ala Val Val Trp Ala Gly Leu Pro Ser Gln Glu Ser Gly Asn 
            500                 505                 510         


Ala Leu Val Asp Val Leu Trp Gly Asp Val Ser Pro Ser Gly Lys Leu 
        515                 520                 525             


Val Tyr Thr Ile Ala Lys Ser Pro Asn Asp Tyr Asn Thr Arg Ile Val 
    530                 535                 540                 


Ser Gly Gly Ser Asp Ser Phe Ser Glu Gly Leu Phe Ile Asp Tyr Lys 
545                 550                 555                 560 


His Phe Asp Asp Ala Asn Ile Thr Pro Arg Tyr Glu Phe Gly Tyr Gly 
                565                 570                 575     


Leu Ser Tyr Thr Lys Phe Asn Tyr Ser Arg Leu Ser Val Leu Ser Thr 
            580                 585                 590         


Ala Lys Ser Gly Pro Ala Thr Gly Ala Val Val Pro Gly Gly Pro Ser 
        595                 600                 605             


Asp Leu Phe Gln Asn Val Ala Thr Val Thr Val Asp Ile Ala Asn Ser 
    610                 615                 620                 


Gly Gln Val Thr Gly Ala Glu Val Ala Gln Leu Tyr Ile Thr Tyr Pro 
625                 630                 635                 640 


Ser Ser Ala Pro Arg Thr Pro Pro Lys Gln Leu Arg Gly Phe Ala Lys 
                645                 650                 655     


Leu Asn Leu Thr Pro Gly Gln Ser Gly Thr Ala Thr Phe Asn Ile Arg 
            660                 665                 670         


Arg Arg Asp Leu Ser Tyr Trp Asp Thr Ala Ser Gln Lys Trp Val Val 
        675                 680                 685             


Pro Ser Gly Ser Phe Gly Ile Ser Val Gly Ala Ser Ser Arg Asp Ile 
    690                 695                 700                 


Arg Leu Thr Ser Thr Leu Ser Val Ala 
705                 710             


<210>  6
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  6
caccatggtg aataacgcag ctc                                               23


<210>  7
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  7
ttatgcgtca ggtgtagcat c                                                 21


<210>  8
<211>  29
<212>  PRT
<213>  Bacillus subtilis

<400>  8

Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 
1               5                   10                  15      


Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala 
            20                  25                  


<210>  9
<211>  32
<212>  PRT
<213>  Trichoderma reesei

<400>  9

Met Val Ser Phe Thr Ser Leu Leu Ala Ala Ser Pro Pro Ser Arg Ala 
1               5                   10                  15      


Ser Cys Arg Pro Ala Ala Glu Val Glu Ser Val Ala Val Glu Lys Arg 
            20                  25                  30          


<210>  10
<211>  16
<212>  PRT
<213>  Trichoderma reesei

<400>  10

Met Lys Ala Asn Val Ile Leu Cys Leu Leu Ala Pro Leu Val Ala Ala 
1               5                   10                  15      


<210>  11
<211>  19
<212>  PRT
<213>  Trichoderma reesei

<400>  11

Met Arg Tyr Arg Thr Ala Ala Ala Leu Ala Leu Ala Thr Gly Pro Phe 
1               5                   10                  15      


Ala Arg Ala 
            


<210>  12
<211>  18
<212>  PRT
<213>  Trichoderma reesei

<400>  12

Met Ile Val Gly Ile Leu Thr Thr Leu Ala Thr Leu Ala Thr Leu Ala 
1               5                   10                  15      


Ala Ser 
        


<210>  13
<211>  17
<212>  PRT
<213>  Trichoderma reesei

<400>  13

Met Tyr Arg Lys Leu Ala Val Ile Ser Ala Phe Leu Ala Thr Ala Arg 
1               5                   10                  15      


Ala 
    


<210>  14
<211>  23
<212>  PRT
<213>  Fusarium verticillioides

<400>  14

Met Leu Leu Asn Leu Gln Val Ala Ala Ser Ala Leu Ser Leu Ser Leu 
1               5                   10                  15      


Leu Gly Gly Leu Ala Glu Ala 
            20              


<210>  15
<211>  19
<212>  PRT
<213>  Fusarium verticillioides

<400>  15

Met Lys Leu Asn Trp Val Ala Ala Ala Leu Ser Ile Gly Ala Ala Gly 
1               5                   10                  15      


Thr Asp Ser 
            


<210>  16
<211>  19
<212>  PRT
<213>  Fusarium verticillioides

<400>  16

Met Ala Ser Ile Arg Ser Val Leu Val Ser Gly Leu Leu Ala Ala Gly 
1               5                   10                  15      


Val Asn Ala 
            


<210>  17
<211>  22
<212>  PRT
<213>  Fusarium verticillioides

<400>  17

Met Trp Leu Thr Ser Pro Leu Leu Phe Ala Ser Thr Leu Leu Gly Leu 
1               5                   10                  15      


Thr Gly Val Ala Leu Ala 
            20          


<210>  18
<211>  16
<212>  PRT
<213>  Fusarium verticillioides

<400>  18

Met Arg Phe Ser Trp Leu Leu Cys Pro Leu Leu Ala Met Gly Ser Ala 
1               5                   10                  15      


<210>  19
<211>  22
<212>  PRT
<213>  Fusarium verticillioides

<400>  19

Met Arg Leu Leu Ser Phe Pro Ser His Leu Leu Val Ala Phe Leu Thr 
1               5                   10                  15      


Leu Lys Glu Ala Ser Ser 
            20          


<210>  20
<211>  20
<212>  PRT
<213>  Fusarium verticillioides

<400>  20

Met Gln Leu Lys Phe Leu Ser Ser Ala Leu Leu Leu Ser Leu Thr Gly 
1               5                   10                  15      


Asn Cys Ala Ala 
            20  


<210>  21
<211>  18
<212>  PRT
<213>  Fusarium verticillioides

<400>  21

Met Lys Val Tyr Trp Leu Val Ala Trp Ala Thr Ser Leu Thr Pro Ala 
1               5                   10                  15      


Leu Ala 
        


<210>  22
<211>  19
<212>  PRT
<213>  Fusarium verticillioides

<400>  22

Met Val Arg Phe Ser Ser Ile Leu Ala Ala Ala Ala Cys Phe Val Ala 
1               5                   10                  15      


Val Glu Ser 
            


<210>  23
<211>  20
<212>  PRT
<213>  Podospora anserine

<400>  23

Met Ile His Leu Lys Pro Ala Leu Ala Ala Leu Leu Ala Leu Ser Thr 
1               5                   10                  15      


Gln Cys Val Ala 
            20  


<210>  24
<211>  17
<212>  PRT
<213>  Podospora anserine

<400>  24

Met Ala Leu Gln Thr Phe Phe Leu Leu Ala Ala Ala Met Leu Ala Asn 
1               5                   10                  15      


Ala 
    


<210>  25
<211>  19
<212>  PRT
<213>  Podospora anserine

<400>  25

Met Lys Leu Asn Lys Pro Phe Leu Ala Ile Tyr Leu Ala Phe Asn Leu 
1               5                   10                  15      


Ala Glu Ala 
            


<210>  26
<211>  20
<212>  PRT
<213>  Chaetomium globosum

<400>  26

Met Ala Pro Leu Ser Leu Arg Ala Leu Ser Leu Leu Ala Leu Thr Gly 
1               5                   10                  15      


Ala Ala Ala Ala 
            20  


<210>  27
<211>  19
<212>  PRT
<213>  Thermoascus aurantiacus

<400>  27

Met Val Arg Pro Thr Ile Leu Leu Thr Ser Leu Leu Leu Ala Pro Phe 
1               5                   10                  15      


Ala Ala Ala 
            


<210>  28
<211>  21
<212>  PRT
<213>  Aspergillus terreus

<400>  28

Met His Met His Ser Leu Val Ala Ala Leu Ala Ala Gly Thr Leu Pro 
1               5                   10                  15      


Leu Leu Ala Ser Ala 
            20      


<210>  29
<211>  19
<212>  PRT
<213>  Aspergillus fumigatus

<400>  29

Met Val His Leu Ser Ser Leu Ala Ala Ala Leu Ala Ala Leu Pro Leu 
1               5                   10                  15      


Val Tyr Gly 
            


<210>  30
<211>  17
<212>  PRT
<213>  Aspergillus fumigatus

<400>  30

Met Arg Phe Ser Leu Ala Ala Thr Thr Leu Leu Ala Gly Leu Ala Thr 
1               5                   10                  15      


Ala 
    


<210>  31
<211>  19
<212>  PRT
<213>  Aspergillus fumigatus

<400>  31

Met Val Val Leu Ser Lys Leu Val Ser Ser Ile Leu Phe Ala Ser Leu 
1               5                   10                  15      


Val Ser Ala 
            


<210>  32
<211>  19
<212>  PRT
<213>  Aspergillus kawachii

<400>  32

Met Val Gln Ile Lys Ala Ala Ala Leu Ala Met Leu Phe Ala Ser His 
1               5                   10                  15      


Val Leu Ser 
            


<210>  33
<211>  17
<212>  PRT
<213>  Magnaporthe grisea

<400>  33

Met Lys Ala Ser Ser Val Leu Leu Gly Leu Ala Pro Leu Ala Ala Leu 
1               5                   10                  15      


Ala 
    


<210>  34
<211>  19
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  34

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala 
            


<210>  35
<211>  85
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  35

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Asp Lys Arg 
                85  


<210>  36
<211>  20
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  36

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Arg 
            20  


