                         SEQUENCE LISTING

<110>  Washington University
       KRUPNICK, Alexander S.
       LAZEAR, Eric R.
       FREMONT, Daved H.
 
<120>  COMPOSITIONS AND METHODS FOR TARGETED CYTOKINE DELIVERY

<130>  047563-508855

<150>  62/091,898
<151>  2014-12-15

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  944
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  1
cacaaactcg cattcaactt caatctagaa ataaatggca gtgatacaca ttctacagta       60

gatgtatatc ttgatgattc tcaaattata acgtttgatg gaaaagatat ccgtccaacc      120

atcccgttca tgataggtga tgaaattttc ttaccgtttt ataaaaatgt gtttagtgag      180

tttttctctc tgtttagaag agttcctaca agtactccat atgaagactt gacatatttt      240

tatgaatgcg actatacaga caataaatct acatttgatc agttttatct ttataatggc      300

gaagaatata ctgtcaaaac acaggaggcc actaataaaa atatgtggct aactacttcc      360

gagtttagac taaaaaaatg gttcgatggc gaagattgta taatgcatct tagatcgtta      420

gttagaaaaa tggaggacag taaacgaaac actggtggta ccggaagtag cggtagtagt      480

gattacaagg acgatgacga caagcaccac catcatcatc atcaccacgg tagcagcggc      540

agcagtgccc ccacctctag cagcacaaag aagacccagc tgcaactgga acacctcctg      600

ctggacctgc agatgatcct gaacggcatc aacaactaca agaaccccaa gctgaccgcc      660

atgctgacca aaaagtttta catgcccaag aaggccaccg agcttaaaca cctgcaatgc      720

cttgaggagg agctgaagcc ctggaggagg tactgaacct ggcccagagc aagaactttc      780

atctgaggcc cagggacctg attagcaaca tcaacgtgat cgtgttggag ttgaagggca      840

gcgagaccac gttcatgtgc gagtacgccg acgagacggc caccatagtg gagtttctta      900

acaggtggat caccttctca cagtctatca tcagcaccct gacc                       944


<210>  2
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  2

His Lys Leu Ala Phe Asn Phe Asn Leu Glu Ile Asn Gly Ser Asp Thr 
1               5                   10                  15      


His Ser Thr Val Asp Val Tyr Leu Asp Asp Ser Gln Ile Ile Thr Phe 
            20                  25                  30          


Asp Gly Lys Asp Ile Arg Pro Thr Ile Pro Phe Met Ile Gly Asp Glu 
        35                  40                  45              


Ile Phe Leu Pro Phe Tyr Lys Asn Val Phe Ser Glu Phe Phe Ser Leu 
    50                  55                  60                  


Phe Arg Arg Val Pro Thr Ser Thr Pro Tyr Glu Asp Leu Thr Tyr Phe 
65                  70                  75                  80  


Tyr Glu Cys Asp Tyr Thr Asp Asn Lys Ser Thr Phe Asp Gln Phe Tyr 
                85                  90                  95      


Leu Tyr Asn Gly Glu Glu Tyr Thr Val Lys Thr Gln Glu Ala Thr Asn 
            100                 105                 110         


Lys Asn Met Trp Leu Thr Thr Ser Glu Phe Arg Leu Lys Lys Trp Phe 
        115                 120                 125             


Asp Gly Glu Asp Cys Ile Met His Leu Arg Ser Leu Val Arg Lys Met 
    130                 135                 140                 


Glu Asp Ser Lys Arg Asn Thr Gly Gly Thr Gly Ser Ser Gly Ser Ser 
145                 150                 155                 160 


Asp Tyr Lys Asp Asp Asp Asp Lys His His His His His His His His 
                165                 170                 175     


Gly Ser Ser Gly Ser Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr 
            180                 185                 190         


Gln Leu Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn 
        195                 200                 205             


Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr Ala Met Leu Thr Lys 
    210                 215                 220                 


Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys 
225                 230                 235                 240 


Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln 
                245                 250                 255     


Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn 
            260                 265                 270         


Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu 
        275                 280                 285             


Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile 
    290                 295                 300                 


Thr Phe Ser Gln Ser Ile Ile Ser Thr Leu Thr 
305                 310                 315 


<210>  3
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  3

Ser Val Tyr Asp Phe Phe Val Trp Leu 
1               5                   


<210>  4
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  4

Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               


<210>  5
<211>  133
<212>  PRT
<213>  Homo sapiens

<400>  5

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  6
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  6

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Ala Met Leu Thr Lys Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  7
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  7

His Lys Leu Ala Phe Asn Phe Asn Leu Glu Ile Asn Gly Ser Asp Thr 
1               5                   10                  15      


His Ser Thr Val Asp Val Tyr Leu Asp Asp Ser Gln Ile Ile Thr Phe 
            20                  25                  30          


Asp Gly Lys Asp Ile Arg Pro Thr Ile Pro Phe Met Ile Gly Asp Glu 
        35                  40                  45              


Ile Phe Leu Pro Phe Tyr Lys Asn Val Phe Ser Glu Phe Phe Ser Leu 
    50                  55                  60                  


Phe Arg Arg Val Pro Thr Ser Thr Pro Tyr Glu Asp Leu Thr Tyr Phe 
65                  70                  75                  80  


Tyr Glu Cys Asp Tyr Thr Asp Asn Lys Ser Thr Phe Asp Gln Phe Tyr 
                85                  90                  95      


Leu Tyr Asn Gly Glu Glu Tyr Thr Val Lys Thr Gln Glu Ala Thr Asn 
            100                 105                 110         


Lys Asn Met Trp Leu Thr Thr Ser Glu Phe Arg Leu Lys Lys Trp Phe 
        115                 120                 125             


Asp Gly Glu Asp Cys Ile Met His Leu Arg Ser Leu Val Arg Lys Met 
    130                 135                 140                 


Glu Asp Ser Lys Arg Asn Thr 
145                 150     


<210>  8
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  8

Gly Ser Ser Gly Ser Ser Asp Tyr Lys Asp Asp Asp Asp Lys His His 
1               5                   10                  15      


His His His His His His Gly Ser Ser Gly Ser Ser 
            20                  25              


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  9

Asp Tyr Lys Asp Asp Asp Lys 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  10

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  11
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  11

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  12

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  13
<211>  150
<212>  PRT
<213>  Cowpox virus

<400>  13

Gly His Lys Leu Ala Phe Asn Phe Asn Leu Glu Ile Asn Gly Ser Asp 
1               5                   10                  15      


Thr His Ser Thr Val Asp Val Tyr Leu Asp Asp Ser Gln Ile Ile Thr 
            20                  25                  30          


Phe Asp Gly Lys Asp Ile Arg Pro Thr Ile Pro Phe Met Ile Gly Asp 
        35                  40                  45              


Glu Ile Phe Leu Pro Phe Tyr Lys Asn Val Phe Ser Glu Phe Phe Ser 
    50                  55                  60                  


Leu Phe Arg Arg Val Pro Thr Ser Thr Pro Tyr Glu Asp Leu Thr Tyr 
65                  70                  75                  80  


Phe Tyr Glu Cys Asp Tyr Thr Asp Asn Lys Ser Thr Phe Asp Gln Phe 
                85                  90                  95      


Tyr Leu Tyr Asn Gly Glu Glu Tyr Thr Val Lys Thr Gln Glu Ala Thr 
            100                 105                 110         


Asn Lys Asn Met Trp Leu Thr Thr Ser Glu Phe Arg Leu Lys Lys Trp 
        115                 120                 125             


Phe Asp Gly Glu Asp Cys Ile Met His Leu Arg Ser Leu Val Arg Lys 
    130                 135                 140                 


Met Glu Asp Ser Lys Arg 
145                 150 


<210>  14
<211>  150
<212>  PRT
<213>  Monkeypox virus

<400>  14

His Lys Leu Val His Tyr Phe Asn Leu Lys Ile Asn Gly Ser Asp Ile 
1               5                   10                  15      


Thr Asn Thr Ala Asp Ile Leu Leu Asp Asn Tyr Pro Ile Met Thr Phe 
            20                  25                  30          


Asp Gly Lys Asp Ile Tyr Pro Ser Ile Ala Phe Met Val Gly Asn Lys 
        35                  40                  45              


Leu Phe Leu Asp Leu Tyr Lys Asn Ile Phe Val Glu Phe Phe Arg Leu 
    50                  55                  60                  


Phe Arg Val Ser Val Ser Ser Gln Tyr Glu Glu Leu Glu Tyr Tyr Tyr 
65                  70                  75                  80  


Ser Cys Asp Tyr Thr Asn Asn Arg Pro Thr Ile Lys Gln His Tyr Phe 
                85                  90                  95      


Tyr Asn Gly Glu Glu Tyr Thr Glu Ile Asp Arg Ser Lys Lys Ala Thr 
            100                 105                 110         


Asn Lys Asn Ser Trp Leu Ile Thr Ser Gly Phe Arg Leu Gln Lys Trp 
        115                 120                 125             


Phe Asp Ser Glu Asp Cys Ile Ile Tyr Leu Arg Ser Leu Val Arg Arg 
    130                 135                 140                 


Met Glu Asp Ser Asn Lys 
145                 150 


<210>  15
<211>  181
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Glu Pro His Ser Leu Arg Tyr Asn Leu Thr Val Leu Ser Trp Asp 
1               5                   10                  15      


Gly Ser Val Gln Ser Gly Phe Leu Thr Glu Val His Leu Asp Gly Gln 
            20                  25                  30          


Pro Phe Leu Arg Cys Arg Asp Arg Gln Lys Cys Arg Ala Lys Pro Gln 
        35                  40                  45              


Gly Gln Trp Ala Glu Asp Val Leu Gly Asn Lys Thr Trp Asp Arg Glu 
    50                  55                  60                  


Thr Arg Asp Leu Thr Gly Asn Gly Lys Asp Leu Arg Met Thr Leu Ala 
65                  70                  75                  80  


His Ile Lys Asp Gln Lys Glu Gly Leu His Ser Leu Gln Glu Ile Arg 
                85                  90                  95      


Val Cys Glu Ile His Glu Asp Asn Ser Thr Arg Ser Ser Gln His Phe 
            100                 105                 110         


Tyr Tyr Asp Gly Glu Leu Phe Leu Ser Gln Asn Leu Glu Thr Lys Glu 
        115                 120                 125             


Trp Thr Met Pro Gln Ser Ser Arg Ala Gln Thr Leu Ala Met Asn Val 
    130                 135                 140                 


Arg Asn Phe Leu Lys Glu Asp Ala Met Lys Thr Lys Thr His Tyr His 
145                 150                 155                 160 


Ala Met His Ala Asp Cys Leu Gln Glu Leu Arg Arg Tyr Leu Lys Ser 
                165                 170                 175     


Gly Val Val Leu Arg 
            180     


<210>  16
<211>  180
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Glu Pro His Ser Leu Arg Tyr Asn Leu Met Val Leu Ser Gln Asp 
1               5                   10                  15      


Gly Ser Val Gln Ser Gly Phe Leu Ala Glu Gly His Leu Asp Gly Gln 
            20                  25                  30          


Pro Phe Leu Arg Tyr Asp Arg Gln Lys Arg Arg Ala Lys Pro Gln Gly 
        35                  40                  45              


Gln Trp Ala Glu Asp Val Leu Gly Ala Glu Thr Trp Asp Thr Glu Thr 
    50                  55                  60                  


Glu Asp Leu Thr Glu Asn Gly Gln Asp Leu Arg Arg Thr Leu Thr His 
65                  70                  75                  80  


Ile Lys Asp Gln Lys Gly Gly Leu His Ser Leu Gln Glu Ile Arg Val 
                85                  90                  95      


Cys Glu Ile His Glu Asp Ser Ser Thr Arg Gly Ser Arg His Phe Tyr 
            100                 105                 110         


Tyr Asn Gly Glu Leu Phe Leu Ser Gln Asn Leu Glu Thr Gln Glu Ser 
        115                 120                 125             


Thr Val Pro Gln Ser Ser Arg Ala Gln Thr Leu Ala Met Asn Val Thr 
    130                 135                 140                 


Asn Phe Trp Lys Glu Asp Ala Met Lys Thr Lys Thr His Tyr Arg Ala 
145                 150                 155                 160 


Met Gln Ala Asp Cys Leu Gln Lys Leu Gln Arg Tyr Leu Lys Ser Gly 
                165                 170                 175     


Val Ala Ile Arg 
            180 


<210>  17
<211>  178
<212>  PRT
<213>  Homo sapiens

<400>  17

Asp Ala His Ser Leu Trp Tyr Asn Phe Thr Ile Ile His Leu Pro Arg 
1               5                   10                  15      


His Gly Gln Gln Trp Cys Glu Val Gln Ser Gln Val Asp Gln Lys Asn 
            20                  25                  30          


Phe Leu Ser Tyr Asp Cys Gly Ser Asp Lys Val Leu Ser Met Gly His 
        35                  40                  45              


Leu Glu Glu Gln Leu Tyr Ala Thr Asp Ala Trp Gly Lys Gln Leu Glu 
    50                  55                  60                  


Met Leu Arg Glu Val Gly Gln Arg Leu Arg Leu Glu Leu Ala Asp Thr 
65                  70                  75                  80  


Glu Leu Glu Asp Phe Thr Pro Ser Gly Pro Leu Thr Leu Gln Val Arg 
                85                  90                  95      


Met Ser Cys Glu Cys Glu Ala Asp Gly Tyr Ile Arg Gly Ser Trp Gln 
            100                 105                 110         


Phe Ser Phe Asp Gly Arg Lys Phe Leu Leu Phe Asp Ser Asn Asn Arg 
        115                 120                 125             


Lys Trp Thr Val Val His Ala Gly Ala Arg Arg Met Lys Glu Lys Trp 
    130                 135                 140                 


Glu Lys Asp Ser Gly Leu Thr Thr Phe Phe Lys Met Val Ser Met Arg 
145                 150                 155                 160 


Asp Cys Lys Ser Trp Leu Arg Asp Phe Leu Met His Arg Lys Lys Arg 
                165                 170                 175     


Leu Glu 
        


<210>  18
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  18

Asp Ala His Ser Leu Arg Cys Asn Leu Thr Ile Lys Asp Pro Thr Pro 
1               5                   10                  15      


Ala Asp Pro Leu Trp Tyr Glu Ala Lys Cys Phe Val Gly Glu Ile Leu 
            20                  25                  30          


Ile Leu His Leu Ser Asn Ile Asn Lys Thr Met Thr Ser Gly Asp Pro 
        35                  40                  45              


Gly Glu Thr Ala Asn Ala Thr Glu Val Lys Lys Cys Leu Thr Gln Pro 
    50                  55                  60                  


Leu Lys Asn Leu Cys Gln Lys Leu Arg Asn Lys Val Ser Asn Thr Lys 
65                  70                  75                  80  


Val Asp Thr His Lys Thr Asn Gly Tyr Pro His Leu Gln Val Thr Met 
                85                  90                  95      


Ile Tyr Pro Gln Ser Gln Gly Arg Thr Pro Ser Ala Thr Trp Glu Phe 
            100                 105                 110         


Asn Ile Ser Asp Ser Tyr Phe Phe Thr Phe Tyr Thr Glu Asn Met Ser 
        115                 120                 125             


Trp Arg Ser Ala Asn Asp Glu Ser Gly Val Ile Met Asn Lys Trp Lys 
    130                 135                 140                 


Asp Asp Gly Glu Phe Val Lys Gln Leu Lys Phe Leu Ile His Glu Cys 
145                 150                 155                 160 


Ser Gln Lys Met Asp Glu Phe Leu Lys Gln Ser Lys Glu Lys 
                165                 170                 


<210>  19
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  19

Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser 
1               5                   10                  15      


<210>  20
<211>  16
<212>  PRT
<213>  Chlorocebus sabaeus

<400>  20

Leu Thr Ile Ile Glu Met Gln Lys Gly Glu Cys Ala Leu Tyr Ala Ser 
1               5                   10                  15      


<210>  21
<211>  16
<212>  PRT
<213>  Callithrix jacchus

<400>  21

Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Val Tyr Ala Ser 
1               5                   10                  15      


<210>  22
<211>  16
<212>  PRT
<213>  Mus musculus

<400>  22

Leu Thr Leu Val Glu Ile Pro Lys Gly Ser Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  23
<211>  16
<212>  PRT
<213>  Rattus norvegicus

<400>  23

Leu Thr Leu Val Lys Thr Pro Ser Gly Thr Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  24
<211>  16
<212>  PRT
<213>  Cavia porcellus

<400>  24

Leu Thr Leu Met Asp Thr Gln Asn Gly Lys Cys Ala Leu Tyr Gly Ser 
1               5                   10                  15      


<210>  25
<211>  16
<212>  PRT
<213>  Spermophilus sensu stricto

<400>  25

Leu Thr Leu Val Glu Met Gln Asn Gly Thr Cys Ile Val Tyr Gly Ser 
1               5                   10                  15      


<210>  26
<211>  16
<212>  PRT
<213>  Peromyscus maniculatus

<400>  26

Leu Thr Val Val Glu Met Gln Ser Gly Ser Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  27
<211>  16
<212>  PRT
<213>  Heterocephalus glaber

<400>  27

Leu Ser Met Val Glu Met Gln Asn Gly Thr Cys Ala Val Tyr Ala Ser 
1               5                   10                  15      


<210>  28
<211>  16
<212>  PRT
<213>  Microtus ochrogaster

<400>  28

Leu Thr Leu Val Glu Met Gln Arg Gly Ser Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  29
<211>  16
<212>  PRT
<213>  Sorex araneus

<400>  29

Val Ser Ile Val Glu Met Gln Gly Gly Asn Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  30
<211>  16
<212>  PRT
<213>  Condylura cristata

<400>  30

Val Thr Val Tyr Glu Met Gln Asn Gly Ser Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  31
<211>  16
<212>  PRT
<213>  Cricetulus griseus

<400>  31

Leu Thr Leu Val Glu Met Gln Asn Gly Ser Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  32
<211>  16
<212>  PRT
<213>  Felis catus

<400>  32

Leu Thr Met Val Asp Met Gln Asn Gly Thr Cys Ala Val Tyr Gly Ser 
1               5                   10                  15      


<210>  33
<211>  5
<212>  PRT
<213>  Cowpox virus

<400>  33

Ala Ser Ser Phe Lys 
1               5   


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SYNTHESIZED

<400>  34

Tyr Ile Asn Met 
1               


