                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
 
<120>  Plant protection from a pest or pathogen by expression of 
       double-stranded RNAs in the plastid

<130>  X3492 PCT S3

<150>  EP 14 19 9415.2
<151>  2014-12-19

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct Nt-ptDP-ACT+SHR

<400>  1
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcagg cacgaggttt ttctgtctag tgagcagtgt ccaacctcaa aagacaacat      180

gtgtgacgac gatgtagcgg ctcttgtcgt agacaatgga tccggtatgt gcaaagccgg      240

tttcgcagga gatgacgcac cccgtgccgt cttcccctcg atcgtcggtc gcccaaggca      300

tcaaggagtc atggtcggta tgggacaaaa ggactcatac gtaggagatg aagcccaaag      360

caaaagaggt atcctcaccc tgaaataccc catcgaacac ggtatcatca ccaactggga      420

tgacatgcaa tgtcatccat catgtcgtgt acattgtcca cgtccaagtt tttatgggct      480

ttcttaagag cttcagctgc atttttcata gattccaata ctgtggtgtt cgtactagct      540

ccctccagag cttctcgttg aagttcaata gtagttaaag tgccatctat ttgcaactga      600

tttttttcta atcgcttctt ccgcttcagc gcttgcatgg ccgctcagat ccccgggtac      660

cgagctcgta tccaagcgct tcgtattcgc ccggagttcg ctcccagaaa tatagccatc      720

cctgccccct cacgtcaatc ccacgagcct cttatccatt ctcattgaac gacggcgggg      780

gagc                                                                   784


<210>  2
<211>  817
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct Nt-ptSL-ACT+SHR

<400>  2
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcgggtggg tggaaaacca cccacccctg caggcacgag gtttttctgt ctagtgagca      180

gtgtccaacc tcaaaagaca acatgtgtga cgacgatgta gcggctcttg tcgtagacaa      240

tggatccggt atgtgcaaag ccggtttcgc aggagatgac gcaccccgtg ccgtcttccc      300

ctcgatcgtc ggtcgcccaa ggcatcaagg agtcatggtc ggtatgggac aaaaggactc      360

atacgtagga gatgaagccc aaagcaaaag aggtatcctc accctgaaat accccatcga      420

acacggtatc atcaccaact gggatgacat gcaatgtcat ccatcatgtc gtgtacattg      480

tccacgtcca agtttttatg ggctttctta agagcttcag ctgcattttt catagattcc      540

aatactgtgg tgttcgtact agctccctcc agagcttctc gttgaagttc aatagtagtt      600

aaagtgccat ctatttgcaa ctgatttttt tctaatcgct tcttccgctt cagcgcttgc      660

atggccgctc agatcccgca cgctcctaat ggagcgtgcg gtatccaagc gcttcgtatt      720

cgcccggagt tcgctcccag aaatatagcc atccctgccc cctcacgtca atcccacgag      780

cctcttatcc attctcattg aacgacggcg ggggagc                               817


<210>  3
<211>  1609
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct Nt-ptHP-ACT+SHR

<400>  3
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcagg cacgaggttt ttctgtctag tgagcagtgt ccaacctcaa aagacaacat      180

gtgtgacgac gatgtagcgg ctcttgtcgt agacaatgga tccggtatgt gcaaagccgg      240

tttcgcagga gatgacgcac cccgtgccgt cttcccctcg atcgtcggtc gcccaaggca      300

tcaaggagtc atggtcggta tgggacaaaa ggactcatac gtaggagatg aagcccaaag      360

caaaagaggt atcctcaccc tgaaataccc catcgaacac ggtatcatca ccaactggga      420

tgacatgcaa tgtcatccat catgtcgtgt acattgtcca cgtccaagtt tttatgggct      480

ttcttaagag cttcagctgc atttttcata gattccaata ctgtggtgtt cgtactagct      540

ccctccagag cttctcgttg aagttcaata gtagttaaag tgccatctat ttgcaactga      600

tttttttcta atcgcttctt ccgcttcagc gcttgcatgg ccgctcagat ctggtacgga      660

ccgtactact ctattcgttt caatatattt atttgtttca gctgactgca agattcaaaa      720

atttctttat tattttaaat tttgtgtcac tcaaaaccag ataaacaatt tgatatagag      780

gcactatata tatacatatt ctcgattata tatgtaaatg agttaacctt ttttttccac      840

ttaaaattat atagggggat ccccggggag cggccatgca agcgctgaag cggaagaagc      900

gattagaaaa aaatcagttg caaatagatg gcactttaac tactattgaa cttcaacgag      960

aagctctgga gggagctagt acgaacacca cagtattgga atctatgaaa aatgcagctg     1020

aagctcttaa gaaagcccat aaaaacttgg acgtggacaa tgtacacgac atgatggatg     1080

acattgcatg tcatcccagt tggtgatgat accgtgttcg atggggtatt tcagggtgag     1140

gatacctctt ttgctttggg cttcatctcc tacgtatgag tccttttgtc ccataccgac     1200

catgactcct tgatgccttg ggcgaccgac gatcgagggg aagacggcac ggggtgcgtc     1260

atctcctgcg aaaccggctt tgcacatacc ggatccattg tctacgacaa gagccgctac     1320

atcgtcgtca cacatgttgt cttttgaggt tggacactgc tcactagaca gaaaaacctc     1380

gtgcgagctc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc     1440

tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac     1500

gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat     1560

caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctac                 1609


<210>  4
<211>  549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct St-ptDP-ACT

<400>  4
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcagg cacgaggttt ttctgtctag tgagcagtgt ccaacctcaa aagacaacat      180

gtgtgacgac gatgtagcgg ctcttgtcgt agacaatgga tccggtatgt gcaaagccgg      240

tttcgcagga gatgacgcac cccgtgccgt cttcccctcg atcgtcggtc gcccaaggca      300

tcaaggagtc atggtcggta tgggacaaaa ggactcatac gtaggagatg aagcccaaag      360

caaaagaggt atcctcaccc tgaaataccc catcgaacac ggtatcatca ccaactggga      420

tgacatgagc tcgtatccaa gcgcttcgta ttcgcccgga gttcgctccc agaaatatag      480

ccatccctgc cccctcacgt caatcccacg agcctcttat ccattctcat tgaacgacgg      540

cgggggagc                                                              549


<210>  5
<211>  472
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct St-ptDP-SHR

<400>  5
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcagg caatgtcatc catcatgtcg tgtacattgt ccacgtccaa gtttttatgg      180

gctttcttaa gagcttcagc tgcatttttc atagattcca atactgtggt gttcgtacta      240

gctccctcca gagcttctcg ttgaagttca atagtagtta aagtgccatc tatttgcaac      300

tgattttttt ctaatcgctt cttccgcttc agcgcttgca tggccgctcg agctcgtatc      360

caagcgcttc gtattcgccc ggagttcgct cccagaaata tagccatccc tgccccctca      420

cgtcaatccc acgagcctct tatccattct cattgaacga cggcggggga gc              472


<210>  6
<211>  784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct St-ptDP-ACT+SHR

<400>  6
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcagg cacgaggttt ttctgtctag tgagcagtgt ccaacctcaa aagacaacat      180

gtgtgacgac gatgtagcgg ctcttgtcgt agacaatgga tccggtatgt gcaaagccgg      240

tttcgcagga gatgacgcac cccgtgccgt cttcccctcg atcgtcggtc gcccaaggca      300

tcaaggagtc atggtcggta tgggacaaaa ggactcatac gtaggagatg aagcccaaag      360

caaaagaggt atcctcaccc tgaaataccc catcgaacac ggtatcatca ccaactggga      420

tgacatgcaa tgtcatccat catgtcgtgt acattgtcca cgtccaagtt tttatgggct      480

ttcttaagag cttcagctgc atttttcata gattccaata ctgtggtgtt cgtactagct      540

ccctccagag cttctcgttg aagttcaata gtagttaaag tgccatctat ttgcaactga      600

tttttttcta atcgcttctt ccgcttcagc gcttgcatgg ccgctcagat ccccgggtac      660

cgagctcgta tccaagcgct tcgtattcgc ccggagttcg ctcccagaaa tatagccatc      720

cctgccccct cacgtcaatc ccacgagcct cttatccatt ctcattgaac gacggcgggg      780

gagc                                                                   784


<210>  7
<211>  905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette of the construct St-ptDP-EPI+PMA

<400>  7
gctcccccgc cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg       60

cagggatggc tatatttctg ggagcgaact ccgggcgaat acgaagcgct tggatacgca      120

tgcctgcaga aagaaggaag tcacgccaga ggacacggag ctgctccaga aggcgcagag      180

caatgtgagc gcatacaaca gcgacgtcac ctcgcgcatc tgctacctga aggtcgacag      240

tctcgagact caagtcgtct cgggcgagaa ctacaagttc cacgtttccg gttgcagcgt      300

caactcggac aaggagctgg gcggctgtgc caatcagaat tgcgagtcat ccaagtacga      360

catcgtcatc tactcgcagt cgtggaccaa cacgctgaag gtgacgtcga ttacgcccgc      420

caacgctggt gccgcaggta actcgtacat gtccatggcg acgcccaacg acgtcaagaa      480

ctacacgaac gacgttggcc agatccagtg ggcgcaggtg ccgctgaacg ccgcgcttga      540

caagctcaag tcgtcccgtg agggtctgac atccgatgag gctgagaagc gtctggccga      600

gtacggcccg aacaagctgc cggaggagaa ggtgaacaag ctgacgctgt tcctgggctt      660

catgtggaac ccgctgtcgt gggccatgga ggtggccgcc gtgctgtcga ttgtgctgct      720

ggattacgct gatttcgcgc tgatcctgtt cctgctgctg ctaaacagat ctcccgggta      780

ccgagctcgt atccaagcgc ttcgtattcg cccggagttc gctcccagaa atatagccat      840

ccctgccccc tcacgtcaat cccacgagcc tcttatccat tctcattgaa cgacggcggg      900

ggagc                                                                  905


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the Prrn promoter; introducing a HindIII site

<400>  8
aaaaaagctt gctcccccgc cgtcgttc                                          28


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the Prrn promoter; introducing an SphI site

<400>  9
aaaagcatgc gtatccaagc gcttcgtat                                         29


<210>  10
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the Prrn promoter; introducing an EcoRI site

<400>  10
aaaagaattc gctcccccgc cgtcgttc                                          28


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the Prrn promoter; introducing a SacI site

<400>  11
aaaagagctc gtatccaagc gcttcgtat                                         29


<210>  12
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the Prrn promoter; introducing a PstI site

<400>  12
aaaactgcag gggtgggtgg ttttccaccc acccgtatcc aagcgcttcg tat              53


<210>  13
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the Prrn promoter; introducing a BamHI site

<400>  13
aaaaggatcc cgcacgctcc attaggagcg tgcggtatcc aagcgcttcg tat              53


<210>  14
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT gene fragment; introducing an SbfI site

<400>  14
aaacctgcag gcacgaggtt tttctgtcta g                                      31


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the ACT gene fragment; introducing a SacI site

<400>  15
aaaagagctc atgtcatccc agttggtgat                                        30


<210>  16
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the SHR gene fragment; introducing an SbfI site

<400>  16
aaaacctgca ggcaatgtca tccatcatgt cg                                     32


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the SHR gene fragment; introducing a SacI site

<400>  17
aaaagagctc gagcggccat gcaagc                                            26


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the E. coli rrnB terminator; introducing a SacI
       site

<400>  18
aaaagagctc atcaaataaa acgaaaggct cagtcg                                 36


<210>  19
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reserve primer of the E. coli rrnB terminator; introducing an 
       EcoRI site

<400>  19
aaaagaattc gtagaaacgc aaaaaggcca tcc                                    33


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT+SHR fragment; introducing a SacI site

<400>  20
aaaagagctc gcacgaggtt tttctgtc                                          28


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the ACT+SHR fragment; introducing a SmaI site

<400>  21
aaaacccggg agcggccatg caagc                                             25


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT gene fragment; introducing an XbaI site

<400>  22
aaaatctaga cacgaggttt ttctgtctag                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT gene fragment; introducing an XhoI site

<400>  23
aaaactcgag cacgaggttt ttctgtctag                                        30


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the ACT gene fragment; introducing a BglI site

<400>  24
aaaagatcta tgtcatccca gttggtgat                                         29


<210>  25
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the SHR gene fragment; introducing an XbaI site

<400>  25
aaaatctaga caatgtcatc catcatgtcg                                        30


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the SHR gene fragment; introducing an XhoI site

<400>  26
aaaactcgag caatgtcatc catcatgtcg                                        30


<210>  27
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the SHR gene fragment; introducing a BamHI site

<400>  27
aaaaggatcc gagcggccat gcaagc                                            26


<210>  28
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT+SHR fragment; introducing the T7 
       promoter sequence; for in vitro dsRNA synthesis

<400>  28
taatacgact cactataggc ctgcaggcac gaggtttttc tgt                         43


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the ACT+SHR fragment; introducing the T7 
       promoter sequence; for in vitro dsRNA synthesis

<400>  29
taatacgact cactataggg gcccgggatc cgatatgcc                              39


<210>  30
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the ACT fragment; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  30
taatacgact cactatagga tgtgtgacga cgatgtagcg                             40


<210>  31
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the ACT fragment; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  31
taatacgact cactataggt tccatgtcat cccagttgg                              39


<210>  32
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the SHR fragment; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  32
taatacgact cactataggg agtggccctg caagccctca a                           41


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the SHR fragment; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  33
taatacgact cactataggg caatgtcatc catcatgtc                              39


<210>  34
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of the gfp gene; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  34
taatacgact cactatagga ggacgacggc aactacaag                              39


<210>  35
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of the gfp gene; introducing the T7 promoter 
       sequence; for in vitro dsRNA synthesis

<400>  35
taatacgact cactataggc tgggtgctca ggtagtggt                              39


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of ACT expression

<400>  36
ccagtcctcc tcactgaagc                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of ACT expression

<400>  37
acgaccagaa gcgtacaagg                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of SHR expression

<400>  38
gatgatttgg acgatgctga                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of SHR expression

<400>  39
tagctggttt gactggcttg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of RPS18 expression (as reference gene)

<400>  40
gcgggagaat gtacagagga                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of RPS18 expression (as reference gene)

<400>  41
aagtcttcac ggagcttgga                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer of RPS4 expression (as reference gene)

<400>  42
cgtcaaagaa acgagcattg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer of RPS4 expression (as reference gene)

<400>  43
tcgctgacac tgtagggttg                                                   20


<210>  44
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  introducing SphI and PstI restriction sites

<400>  44
gcatgcctgc ag                                                           12


