               SEQUENCE LISTING

<110> Global Bioenergies
      Scientist Of Fortune S.A.

<120> Enzymatic production of acrylyl-CoA from glycerol

<130> X3295 PCT S3

<150> EP14 19 9144.8
<151> 2014-12-19

<160> 43

<170> BiSSAP 1.2

<210> 1
<211> 422
<212> PRT
<213> Jeotgalibacillus sp. ATCC 8456


<400> 1
Met Ala Thr Leu Lys Arg Asp Lys Gly Leu Asp Asn Thr Leu Lys Val 
1               5                   10                  15      
Leu Lys Gln Gly Tyr Leu Tyr Thr Thr Asn Gln Arg Asn Arg Leu Asn 
            20                  25                  30          
Thr Ser Val Phe Gln Thr Lys Ala Leu Gly Gly Lys Pro Phe Val Val 
        35                  40                  45              
Val Thr Gly Lys Glu Gly Ala Glu Met Phe Tyr Asn Asn Asp Val Val 
    50                  55                  60                  
Gln Arg Glu Gly Met Leu Pro Lys Arg Ile Val Asn Thr Leu Phe Gly 
65                  70                  75                  80  
Lys Gly Ala Ile His Thr Val Asp Gly Lys Lys His Val Asp Arg Lys 
                85                  90                  95      
Ala Leu Phe Met Ser Leu Met Thr Glu Gly Asn Leu Asn Tyr Val Arg 
            100                 105                 110         
Glu Leu Thr Arg Thr Leu Trp His Ala Asn Thr Gln Arg Met Glu Ser 
        115                 120                 125             
Met Asp Glu Val Asn Ile Tyr Arg Glu Ser Ile Val Leu Leu Thr Lys 
    130                 135                 140                 
Val Gly Thr Arg Trp Ala Gly Val Gln Ala Pro Pro Glu Asp Ile Glu 
145                 150                 155                 160 
Arg Ile Ala Thr Asp Met Asp Ile Met Ile Asp Ser Phe Arg Ala Leu 
                165                 170                 175     
Gly Gly Ala Phe Lys Gly Tyr Lys Ala Ser Lys Glu Ala Arg Arg Arg 
            180                 185                 190         
Val Glu Asp Trp Leu Glu Glu Gln Ile Ile Glu Thr Arg Lys Gly Asn 
        195                 200                 205             
Ile His Pro Pro Glu Gly Thr Ala Leu Tyr Glu Phe Ala His Trp Glu 
    210                 215                 220                 
Asp Tyr Leu Gly Asn Pro Met Asp Ser Arg Thr Cys Ala Ile Asp Leu 
225                 230                 235                 240 
Met Asn Thr Phe Arg Pro Leu Ile Ala Ile Asn Arg Phe Val Ser Phe 
                245                 250                 255     
Gly Leu His Ala Met Asn Glu Asn Pro Ile Thr Arg Glu Lys Ile Lys 
            260                 265                 270         
Ser Glu Pro Asp Tyr Ala Tyr Lys Phe Ala Gln Glu Val Arg Arg Tyr 
        275                 280                 285             
Tyr Pro Phe Val Pro Phe Leu Pro Gly Lys Ala Lys Val Asp Ile Asp 
    290                 295                 300                 
Phe Gln Gly Val Thr Ile Pro Ala Gly Val Gly Leu Ala Leu Asp Val 
305                 310                 315                 320 
Tyr Gly Thr Thr His Asp Glu Ser Leu Trp Asp Asp Pro Asn Glu Phe 
                325                 330                 335     
Arg Pro Glu Arg Phe Glu Thr Trp Asp Gly Ser Pro Phe Asp Leu Ile 
            340                 345                 350         
Pro Gln Gly Gly Gly Asp Tyr Trp Thr Asn His Arg Cys Ala Gly Glu 
        355                 360                 365             
Trp Ile Thr Val Ile Ile Met Glu Glu Thr Met Lys Tyr Phe Ala Glu 
    370                 375                 380                 
Lys Ile Thr Tyr Asp Val Pro Glu Gln Asp Leu Glu Val Asp Leu Asn 
385                 390                 395                 400 
Ser Ile Pro Gly Tyr Val Lys Ser Gly Phe Val Ile Lys Asn Val Arg 
                405                 410                 415     
Glu Val Val Asp Arg Thr 
            420         

<210> 2
<211> 441
<212> PRT
<213> Macrococcus caseolyticus (strain JCSC5402)


<400> 2
Met Arg Val Glu Phe Thr Ile Asn Tyr Ile Asn Val Glu Gly Ile Ser 
1               5                   10                  15      
Met Ser Lys Arg Val Pro Lys Asp Arg Gly Ile Asp Asn Ser Leu Lys 
            20                  25                  30          
Ile Met Lys Glu Gly Tyr Glu Tyr Val Pro Ala Arg Met Lys Lys Phe 
        35                  40                  45              
Asn Thr Asn Ile Phe Glu Thr Arg Val Leu Gly Gly Lys Thr Ala Val 
    50                  55                  60                  
Val Ile Ser Gly Lys Asp Ala Ala Glu Leu Phe Tyr Asp Asn Asp Lys 
65                  70                  75                  80  
Thr Glu Arg Lys Gly Thr Leu Pro Lys Arg Val Val Lys Thr Leu Phe 
                85                  90                  95      
Gly Lys Gly Ala Ile His Thr Thr Thr Gly Lys Lys His Ile Asp Arg 
            100                 105                 110         
Lys Ala Leu Phe Met Ser Leu Met Thr Asp Glu Asn Leu Ala Tyr Leu 
        115                 120                 125             
Arg Lys Leu Thr Arg Ile Tyr Trp Phe Gln Asn Ile Glu His Met Gln 
    130                 135                 140                 
Tyr Lys Gln Lys Val Asn Val Tyr Glu Glu Ala Thr Glu Leu Leu Thr 
145                 150                 155                 160 
Lys Val Gly Leu Arg Trp Ala Gly Ile Val Asp His Pro Glu Asn Ile 
                165                 170                 175     
Gln Lys Met Ala Asp Asp Met Asn Lys Met Ile Asp Ser Phe Ser Ala 
            180                 185                 190         
Ile Gly Ser Leu Tyr Gly Gly Tyr Arg Glu Ala Lys Lys Ala Arg Ala 
        195                 200                 205             
Arg Val Glu Gln Phe Leu Glu Asp Gln Ile Thr Ala Val Arg Lys Gly 
    210                 215                 220                 
Lys Ile His Pro Glu Lys Gly Thr Ala Leu Tyr Glu Phe Ser His Trp 
225                 230                 235                 240 
Glu Asp Met Asn Gly Lys Pro Met Asp Ala Arg Leu Cys Ala Val Asp 
                245                 250                 255     
Leu Met Asn Val Ile Arg Pro Leu Val Ala Ile Asn Lys Phe Val Ser 
            260                 265                 270         
Phe Gly Val Leu Ala Leu His Glu Phe Pro Gly Glu Arg Val Arg Val 
        275                 280                 285             
Ala Leu Asn Glu Gly Asp Tyr Ala Tyr Lys Phe Val Gln Glu Val Arg 
    290                 295                 300                 
Arg Tyr Tyr Pro Phe Val Pro Phe Leu Pro Gly Lys Ala Lys Glu Asn 
305                 310                 315                 320 
Ile Thr Phe Asp Gly Tyr Lys Ile Gln Lys Asp Thr Met Met Leu Leu 
                325                 330                 335     
Asp Ile Tyr Gly Thr Leu His Arg Asp Asp Leu Phe Ser Glu Pro Glu 
            340                 345                 350         
Arg Phe Asn Pro Tyr Arg Phe Asp Asn Trp Asp Gly Ser Pro Phe Asp 
        355                 360                 365             
Leu Ile Pro Gln Gly Gly Gly Asp Tyr Tyr Thr Asn His Arg Cys Ala 
    370                 375                 380                 
Gly Glu Trp Met Thr Ile Ile Ile Met Glu Glu Thr Met Lys Phe Phe 
385                 390                 395                 400 
Ala Asn Glu Ile Ser Tyr Asp Val Pro Pro Gln Asp Phe Thr Val Asp 
                405                 410                 415     
Thr Thr Lys Phe Pro Gly Lys Val Ala Ser Gly Met Asp Ile Glu Asn 
            420                 425                 430         
Ile Arg Val Asn Ile Asp Arg Thr Lys 
        435                 440     

<210> 3
<211> 527
<212> PRT
<213> Rhodotorula minuta


<400> 3
Met Gly Ile Val Gln Glu Ala Ala Ala Phe Val Gly Ser Leu Thr Ala 
1               5                   10                  15      
Val Gln Leu Ala Gly Ala Phe Ala Ala Leu Val Leu Ala Phe His Val 
            20                  25                  30          
Val Pro Tyr Ile Leu Asp Pro His Gly Leu Arg Lys Tyr Pro Ala Ala 
        35                  40                  45              
Gly Pro Phe Gly Ile Ala Ala Phe Thr Asp Ala Trp Val Val Trp Gln 
    50                  55                  60                  
Ala Arg Arg Gly Lys Arg Phe Glu Ala Leu Asp Lys Ala His Arg Lys 
65                  70                  75                  80  
Tyr Gly Lys Tyr Val Arg Ile Ser Pro Arg Gln Val Ser Ile Ala Asp 
                85                  90                  95      
Pro Lys Ala Leu Asp Thr Ile Tyr Gly His Gly Thr Gly Thr Met Lys 
            100                 105                 110         
Pro Ala Phe Tyr Asp Ala Phe Val Gly Leu Lys Asn Val Arg Gly Leu 
        115                 120                 125             
Phe Asn Thr Arg Asp Arg Ala Glu His Thr Arg Lys Arg Lys Ile Val 
    130                 135                 140                 
Ser Ala Thr Phe Ala Gln Arg Asn Val Leu Glu Phe Glu Pro Tyr Ile 
145                 150                 155                 160 
Ala Ser Val Met Arg Gln Leu Leu Asn Lys Trp Asp Ala Met Cys Asp 
                165                 170                 175     
Lys Ala Val Lys Asp Gly Lys Gly Trp Val Thr Leu Asp Thr Leu Thr 
            180                 185                 190         
Trp Leu Asn Phe Leu Ala Phe Asp Ile Ile Gly Asp Leu Ala Phe Gly 
        195                 200                 205             
Ala Pro Phe Gly Met Val Glu Arg Glu Ala Asp Ile Cys Glu Ile Glu 
    210                 215                 220                 
Gln Glu Asp Gly Ser Ile Lys His Leu Pro Ala Val Thr Ile Leu Asn 
225                 230                 235                 240 
Glu Arg Gly Glu Tyr Ser Asn Cys Leu Gly Val Met Pro Pro His Trp 
                245                 250                 255     
Arg Pro Phe Ser Lys Tyr Val Asp Pro Trp Phe Ser Arg Gly His Ala 
            260                 265                 270         
Ser Val Ile Asn Leu Ala Gly Met Ala Arg Ala Arg Val Asn Ala Arg 
        275                 280                 285             
Leu Lys Ser Gly Ala Gly Asp Arg Lys Asp Leu Leu Ala Arg Leu Gln 
    290                 295                 300                 
Glu Ala Arg Asp Glu Ser Gly Asn Pro Met Asp Ile Asp Glu Leu Thr 
305                 310                 315                 320 
Ala Glu Ala Leu Thr Gln Leu Ile Ala Gly Ser Asp Thr Thr Ser Asn 
                325                 330                 335     
Ser Ser Cys Ala Ile Ile Phe Tyr Leu Ala Arg Asn Gln Asp Ala Gln 
            340                 345                 350         
Lys Lys Leu Gln Ala Glu Leu Asp Lys Thr Phe Lys Asp Arg Gly Ile 
        355                 360                 365             
Ser Gly Val Met Glu Tyr Glu Asp Ile Lys Thr Leu Pro Tyr Leu Glu 
    370                 375                 380                 
Ala Cys Ile Asn Glu Ala Leu Arg Met His Ser Thr Ser Ser Met Gly 
385                 390                 395                 400 
Leu Pro Arg Val Met Pro Ala Gln Gly Ala Asp Phe Gln Gly Glu His 
                405                 410                 415     
Phe Lys Glu Gly Thr Glu Val Ser Val Pro Ala Tyr Thr Ile His His 
            420                 425                 430         
Leu Glu Ser Ile Trp Gly Asp Pro Phe Asn Tyr Arg Pro Glu Arg Trp 
        435                 440                 445             
Leu Gly Ser Asp Ala Lys Gln Leu Glu Lys Ser Phe Ile Pro Phe Ser 
    450                 455                 460                 
Ile Gly Pro Arg Ser Cys Val Gly Arg Asn Leu Ala Thr Met Glu Leu 
465                 470                 475                 480 
Leu Val Phe Met Ser Thr Leu Phe Tyr Arg Tyr Asp Phe Lys Leu Ala 
                485                 490                 495     
Asp Glu Asn Gln Thr Lys Leu Glu Thr Ser Glu Gly Phe Leu Arg Lys 
            500                 505                 510         
Pro Leu Glu Ser Trp Ile Lys Met Arg Arg Arg Ser Ile Asp Ala 
        515                 520                 525         

<210> 4
<211> 132
<212> PRT
<213> Escherichia coli (strain K12) 


<400> 4
Met Ser Thr Thr His Asn Val Pro Gln Gly Asp Leu Val Leu Arg Thr 
1               5                   10                  15      
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 
            20                  25                  30          
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu 
        35                  40                  45              
Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe 
    50                  55                  60                  
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys 
65                  70                  75                  80  
Val Gln Lys Gly Thr Thr Ser Val Ser Ile Asn Ile Glu Val Trp Val 
                85                  90                  95      
Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu 
            100                 105                 110         
Ala Leu Phe Lys Tyr Val Ala Val Asp Pro Glu Gly Lys Pro Arg Ala 
        115                 120                 125             
Leu Pro Val Glu 
    130         

<210> 5
<211> 286
<212> PRT
<213> Escherichia coli (strain K12) 


<400> 5
Met Ser Gln Ala Leu Lys Asn Leu Leu Thr Leu Leu Asn Leu Glu Lys 
1               5                   10                  15      
Ile Glu Glu Gly Leu Phe Arg Gly Gln Ser Glu Asp Leu Gly Leu Arg 
            20                  25                  30          
Gln Val Phe Gly Gly Gln Val Val Gly Gln Ala Leu Tyr Ala Ala Lys 
        35                  40                  45              
Glu Thr Val Pro Glu Glu Arg Leu Val His Ser Phe His Ser Tyr Phe 
    50                  55                  60                  
Leu Arg Pro Gly Asp Ser Lys Lys Pro Ile Ile Tyr Asp Val Glu Thr 
65                  70                  75                  80  
Leu Arg Asp Gly Asn Ser Phe Ser Ala Arg Arg Val Ala Ala Ile Gln 
                85                  90                  95      
Asn Gly Lys Pro Ile Phe Tyr Met Thr Ala Ser Phe Gln Ala Pro Glu 
            100                 105                 110         
Ala Gly Phe Glu His Gln Lys Thr Met Pro Ser Ala Pro Ala Pro Asp 
        115                 120                 125             
Gly Leu Pro Ser Glu Thr Gln Ile Ala Gln Ser Leu Ala His Leu Leu 
    130                 135                 140                 
Pro Pro Val Leu Lys Asp Lys Phe Ile Cys Asp Arg Pro Leu Glu Val 
145                 150                 155                 160 
Arg Pro Val Glu Phe His Asn Pro Leu Lys Gly His Val Ala Glu Pro 
                165                 170                 175     
His Arg Gln Val Trp Ile Arg Ala Asn Gly Ser Val Pro Asp Asp Leu 
            180                 185                 190         
Arg Val His Gln Tyr Leu Leu Gly Tyr Ala Ser Asp Leu Asn Phe Leu 
        195                 200                 205             
Pro Val Ala Leu Gln Pro His Gly Ile Gly Phe Leu Glu Pro Gly Ile 
    210                 215                 220                 
Gln Ile Ala Thr Ile Asp His Ser Met Trp Phe His Arg Pro Phe Asn 
225                 230                 235                 240 
Leu Asn Glu Trp Leu Leu Tyr Ser Val Glu Ser Thr Ser Ala Ser Ser 
                245                 250                 255     
Ala Arg Gly Phe Val Arg Gly Glu Phe Tyr Thr Gln Asp Gly Val Leu 
            260                 265                 270         
Val Ala Ser Thr Val Gln Glu Gly Val Met Arg Asn His Asn 
        275                 280                 285     

<210> 6
<211> 289
<212> PRT
<213> Pseudomonas putida


<400> 6
Met Ser His Val Leu Asp Asp Leu Val Asp Leu Leu Ser Leu Glu Ser 
1               5                   10                  15      
Ile Glu Glu Asn Leu Phe Arg Gly Arg Ser Gln Asp Leu Gly Phe Arg 
            20                  25                  30          
Gln Leu Tyr Gly Gly Gln Val Leu Gly Gln Ser Leu Ser Ala Ala Ser 
        35                  40                  45              
Gln Thr Val Glu Asp Ala Arg His Val His Ser Leu His Gly Tyr Phe 
    50                  55                  60                  
Leu Arg Pro Gly Asp Ala Ser Leu Pro Val Val Tyr Ser Val Asp Arg 
65                  70                  75                  80  
Val Arg Asp Gly Gly Ser Phe Ser Thr Arg Arg Val Thr Ala Ile Gln 
                85                  90                  95      
Lys Gly Gln Thr Ile Phe Thr Cys Ser Ala Ser Phe Gln Tyr Asp Glu 
            100                 105                 110         
Glu Gly Phe Glu His Gln Ala Gln Met Pro Asp Val Val Gly Pro Glu 
        115                 120                 125             
Asn Leu Pro Thr Glu Val Glu Leu Ala His Ala Met Ala Asp Gln Leu 
    130                 135                 140                 
Pro Glu Arg Ile Arg Asp Lys Val Leu Cys Ala Lys Pro Ile Glu Ile 
145                 150                 155                 160 
Arg Pro Val Thr Glu Arg Asp Pro Phe Asn Pro Lys Pro Gly Asp Pro 
                165                 170                 175     
Val Lys Tyr Ala Trp Phe Arg Ala Asp Gly Asn Leu Pro Asp Val Pro 
            180                 185                 190         
Ala Leu His Lys Tyr Met Leu Ala Tyr Ala Ser Asp Phe Gly Leu Leu 
        195                 200                 205             
Thr Thr Ala Leu Leu Pro His Gly Lys Ser Val Trp Gln Arg Asp Met 
    210                 215                 220                 
Gln Ile Ala Ser Leu Asp His Ser Leu Trp Phe His Gly Asn Leu Arg 
225                 230                 235                 240 
Ala Asp Gln Trp Leu Leu Tyr Ala Thr Asp Ser Pro Trp Ala Gly Asn 
                245                 250                 255     
Ser Arg Gly Phe Cys Arg Gly Ser Ile Phe Asn Gln Ala Gly Gln Leu 
            260                 265                 270         
Val Ala Ser Ser Ser Gln Glu Gly Leu Ile Arg His Arg Lys Asp Trp 
        275                 280                 285             
Ala 
    

<210> 7
<211> 154
<212> PRT
<213> Haemophilus influenzae (strain R2866)

<220> 
<223> Acyl-CoA thioesterase YciA

<400> 7
Met Ser Ala Asn Phe Thr Asp Lys Asn Gly Arg Gln Ser Lys Gly Val 
1               5                   10                  15      
Leu Leu Leu Arg Thr Leu Ala Met Pro Ser Asp Thr Asn Ala Asn Gly 
            20                  25                  30          
Asp Ile Phe Gly Gly Trp Ile Met Ser Gln Met Asp Met Gly Gly Ala 
        35                  40                  45              
Ile Leu Ala Lys Glu Ile Ala His Gly Arg Val Val Thr Val Ala Val 
    50                  55                  60                  
Glu Ser Met Asn Phe Ile Lys Pro Ile Ser Val Gly Asp Val Val Cys 
65                  70                  75                  80  
Cys Tyr Gly Glu Cys Leu Lys Val Gly Arg Ser Ser Ile Lys Ile Lys 
                85                  90                  95      
Val Glu Val Trp Val Lys Lys Val Ala Ser Glu Pro Ile Gly Glu Arg 
            100                 105                 110         
Tyr Cys Val Thr Asp Ala Val Phe Thr Phe Val Ala Val Asp Asn Asn 
        115                 120                 125             
Gly Arg Ser Arg Thr Ile Pro Arg Glu Asn Asn Gln Glu Leu Glu Lys 
    130                 135                 140                 
Ala Leu Ala Leu Ile Ser Glu Gln Pro Leu 
145                 150                 

<210> 8
<211> 140
<212> PRT
<213> Homo sapiens

<220> 
<223> Acyl-coenzyme A thioesterase

<400> 8
Met Thr Ser Met Thr Gln Ser Leu Arg Glu Val Ile Lys Ala Met Thr 
1               5                   10                  15      
Lys Ala Arg Asn Phe Glu Arg Val Leu Gly Lys Ile Thr Leu Val Ser 
            20                  25                  30          
Ala Ala Pro Gly Lys Val Ile Cys Glu Met Lys Val Glu Glu Glu His 
        35                  40                  45              
Thr Asn Ala Ile Gly Thr Leu His Gly Gly Leu Thr Ala Thr Leu Val 
    50                  55                  60                  
Asp Asn Ile Ser Thr Met Ala Leu Leu Cys Thr Glu Arg Gly Ala Pro 
65                  70                  75                  80  
Gly Val Ser Val Asp Met Asn Ile Thr Tyr Met Ser Pro Ala Lys Leu 
                85                  90                  95      
Gly Glu Asp Ile Val Ile Thr Ala His Val Leu Lys Gln Gly Lys Thr 
            100                 105                 110         
Leu Ala Phe Thr Ser Val Asp Leu Thr Asn Lys Ala Thr Gly Lys Leu 
        115                 120                 125             
Ile Ala Gln Gly Arg His Thr Lys His Leu Gly Asn 
    130                 135                 140 

<210> 9
<211> 259
<212> PRT
<213> Metallosphaera cuprina Ar-4

<220> 
<223> 3-hydroxypropionyl-CoA dehydratase

<400> 9
Met Glu Tyr Glu Thr Leu Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp 
1               5                   10                  15      
Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ser Lys Leu 
            20                  25                  30          
Leu Glu Glu Leu Asn Arg Ala Val Ser Glu Gly Glu Ser Asp Pro Glu 
        35                  40                  45              
Val Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly 
    50                  55                  60                  
Ala Asp Ile Thr Gln Phe Asn Gln Leu Ser Pro Thr Asp Ala Trp Arg 
65                  70                  75                  80  
Phe Ser Lys Arg Gly Arg Glu Val Met Asp Lys Ile Glu Ser Leu Ser 
                85                  90                  95      
Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu 
            100                 105                 110         
Glu Leu Ala Leu Ser Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln 
        115                 120                 125             
Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly 
    130                 135                 140                 
Thr Gln Arg Leu Thr Arg Ile Val Gly Lys Gly Arg Ala Leu Glu Ile 
145                 150                 155                 160 
Met Met Thr Gly Asp Arg Leu Ser Gly Lys Asp Ala Glu Arg Tyr Gly 
                165                 170                 175     
Leu Val Asn Arg Val Thr Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg 
            180                 185                 190         
Lys Leu Ala Glu Lys Ile Ala Arg Lys Ser Pro Val Ser Leu Ala Leu 
        195                 200                 205             
Ile Lys Glu Val Val Asn Lys Gly Leu Asp Ser Pro Leu Ala Ser Gly 
    210                 215                 220                 
Leu Ser Leu Glu Ser Ile Gly Trp Gly Val Ile Phe Ser Thr Glu Asp 
225                 230                 235                 240 
Lys Lys Glu Gly Val Asn Ala Phe Leu Glu Lys Arg Glu Pro Asn Phe 
                245                 250                 255     
Lys Gly Lys 
            

<210> 10
<211> 259
<212> PRT
<213> Metallosphaera sedula

<220> 
<223> 3-hydroxypropionyl-coenzyme A dehydratase

<400> 10
Met Glu Phe Glu Thr Ile Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp 
1               5                   10                  15      
Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ala Lys Leu 
            20                  25                  30          
Leu Glu Glu Leu Asp Arg Ala Val Ser Gln Ala Glu Ser Asp Pro Glu 
        35                  40                  45              
Ile Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly 
    50                  55                  60                  
Ala Asp Ile Thr Gln Phe Asn Gln Leu Thr Pro Ala Glu Ala Trp Lys 
65                  70                  75                  80  
Phe Ser Lys Lys Gly Arg Glu Ile Met Asp Lys Ile Glu Ala Leu Ser 
                85                  90                  95      
Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu 
            100                 105                 110         
Glu Leu Ala Leu Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln 
        115                 120                 125             
Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly 
    130                 135                 140                 
Thr Gln Arg Leu Thr Arg Val Ile Gly Lys Gly Arg Ala Leu Glu Met 
145                 150                 155                 160 
Met Met Thr Gly Asp Arg Ile Pro Gly Lys Asp Ala Glu Lys Tyr Gly 
                165                 170                 175     
Leu Val Asn Arg Val Val Pro Leu Ala Asn Leu Glu Gln Glu Thr Arg 
            180                 185                 190         
Lys Leu Ala Glu Lys Ile Ala Lys Lys Ser Pro Ile Ser Leu Ala Leu 
        195                 200                 205             
Ile Lys Glu Val Val Asn Arg Gly Leu Asp Ser Pro Leu Leu Ser Gly 
    210                 215                 220                 
Leu Ala Leu Glu Ser Val Gly Trp Gly Val Val Phe Ser Thr Glu Asp 
225                 230                 235                 240 
Lys Lys Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Glu Pro Thr Phe 
                245                 250                 255     
Lys Gly Lys 
            

<210> 11
<211> 257
<212> PRT
<213> Sulfolobus tokodaii str. 7

<220> 
<223> 3-hydroxypropionyl-CoA dehydratase

<400> 11
Met Glu Thr Ile Val Ile Lys Lys Glu Thr Pro Ile Gly Trp Ile Tyr 
1               5                   10                  15      
Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Gln Gln Met Ile Lys 
            20                  25                  30          
Glu Leu Arg Gln Gly Ile Asp Glu Met Val Tyr Asp Ser Asp Ile Lys 
        35                  40                  45              
Val Ile Ile Ile Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Arg Phe Lys Glu Leu Asn Gly Tyr Thr Ala Trp Gln Phe Ala 
65                  70                  75                  80  
Lys Ser Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Ile Ser Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Val Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Met Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Val Arg Leu Ile Gly Lys Gly Lys Ala Leu Glu Leu Met Leu 
145                 150                 155                 160 
Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Lys Ile Gly Leu Val 
                165                 170                 175     
Asn Lys Val Val Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg Asn Phe 
            180                 185                 190         
Ala Leu Lys Leu Ala Glu Lys Pro Pro Ile Ser Ile Ala Leu Ile Lys 
        195                 200                 205             
Leu Leu Val Asn Gln Gly Ile Asp Leu Pro Ile Leu Ala Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Val Phe Ser Thr Glu Asp Glu Lys 
225                 230                 235                 240 
Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Gln Phe Lys Gly 
                245                 250                 255     
Lys 
    

<210> 12
<211> 258
<212> PRT
<213> Brevibacillus laterosporus LMG 15441

<220> 
<223> 3-hydroxypropionyl-coenzyme A dehydratase

<400> 12
Met Ser Tyr Pro Asn Leu Thr Leu Val Thr Glu Gly Thr Ile Ala Ile 
1               5                   10                  15      
Val Thr Ile Asn His Pro Pro Ala Asn Ala Leu Asn Gln Ala Thr Leu 
            20                  25                  30          
Thr Ser Leu Ala Gln Ala Leu Asp Asp Leu Glu Gln Asn Asp Gln Ile 
        35                  40                  45              
Arg Ala Ile Val Ile Thr Gly Glu Gly Arg Phe Phe Ile Ala Gly Ala 
    50                  55                  60                  
Asp Ile Lys Glu Phe Thr Ala Leu Ala Glu Gln Ser Pro Gln Gln Val 
65                  70                  75                  80  
Ala Glu Arg Gly Gln Gln Leu Phe Leu Arg Met Glu Thr Phe Ser Lys 
                85                  90                  95      
Pro Ile Ile Ala Ala Ile Asn Gly Ala Cys Leu Gly Gly Gly Leu Glu 
            100                 105                 110         
Leu Ala Met Ala Cys His Ile Arg Tyr Val Ala Lys Glu Ala Lys Leu 
        115                 120                 125             
Gly Leu Pro Glu Leu Asn Leu Gly Leu Ile Pro Gly Tyr Gly Gly Thr 
    130                 135                 140                 
Gln Arg Leu Pro Arg Leu Ile Gly Arg Gly Lys Ala Thr Gln Leu Ile 
145                 150                 155                 160 
Leu Thr Ser Asp Met Ile Asp Gly Glu Glu Ala Leu Ala Ile Gly Leu 
                165                 170                 175     
Ala Glu Ala Val Tyr Pro Val Glu Gln Leu Leu Glu Glu Ser Lys Lys 
            180                 185                 190         
Leu Ala Arg Lys Ile Ser Glu Lys Gly Ala Ile Ser Val Lys Tyr Ala 
        195                 200                 205             
Leu Asp Ala Ile His Ser Gly Val Glu Leu Gly Leu Ser Ala Gly Met 
    210                 215                 220                 
Lys Arg Glu Ala Glu Leu Phe Gly Gln Val Phe Thr Thr Glu Asp Met 
225                 230                 235                 240 
Lys Glu Gly Val Thr Ala Phe Leu Glu Lys Arg Lys Pro Gln Phe Ser 
                245                 250                 255     
Asn Arg 
        

<210> 13
<211> 257
<212> PRT
<213> Sulfolobus acidocaldarius DSM 639

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 13
Met Glu Thr Ile Ile Val Lys Asn Glu Pro Pro Phe Leu Arg Ile Thr 
1               5                   10                  15      
Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Lys Lys Met Ile Glu 
            20                  25                  30          
Glu Ile Arg Thr Val Leu Glu Glu Thr Val Lys Asp Glu Lys Val Arg 
        35                  40                  45              
Val Ile Ile Phe Thr Gly Asn Gly Arg Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Gln Phe Lys Glu Leu Glu Gly Leu Thr Ala Trp Gln Phe Ala 
65                  70                  75                  80  
Met Lys Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Tyr Pro Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Leu Ala Cys Asp Ile Arg Ile Ala Ser Asp Glu Ala Gln Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Thr Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Leu Lys Leu Val Gly Lys Ser Arg Thr Leu Glu Met Ile Met 
145                 150                 155                 160 
Leu Gly Glu Arg Ile Ser Ala Lys Asp Ala Glu Arg Ile Gly Leu Val 
                165                 170                 175     
Asn Arg Val Val Pro Ser Asn Asp Leu Glu Lys Glu Thr Leu Asn Leu 
            180                 185                 190         
Ala Ser Lys Leu Ala Glu Arg Pro Pro Leu Ala Ile Gln Leu Ser Lys 
        195                 200                 205             
Leu Ile Val Asn Gln Gly Met Asn Ser Pro Ile Thr Val Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Ile Phe Thr Thr Lys Asp Ser Lys 
225                 230                 235                 240 
Glu Gly Val Asn Ala Phe Leu Glu Lys Arg Lys Pro Asn Phe Lys Gly 
                245                 250                 255     
Glu 
    

<210> 14
<211> 257
<212> PRT
<213> Acidianus hospitalis W1

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 14
Met Glu Thr Val Glu Thr Lys Ile Glu Asn Gly Ile Gly Trp Ile Ile 
1               5                   10                  15      
Leu Asn Arg Pro Asp Lys Leu Asn Ala Ile Asn Leu Lys Met Leu Glu 
            20                  25                  30          
Glu Leu Glu Glu Val Thr Lys Asn Phe Glu Glu Asn Asn Asp Val Lys 
        35                  40                  45              
Ile Ile Ile Phe Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Gln Phe Lys Glu Leu Asn Ser Ile Ser Ala Trp Asn Phe Ala 
65                  70                  75                  80  
Lys Lys Gly Arg Arg Val Met Asp Tyr Ile Glu Ser Val Ser Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Leu Ala Cys Asp Phe Arg Ile Ala Ala Glu Glu Ala Ser Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Val Arg Ala Ile Gly Lys Ala Lys Ala Met Glu Leu Met Met 
145                 150                 155                 160 
Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Arg Ile Gly Leu Val 
                165                 170                 175     
Asn Lys Val Val Ser Leu Ser Ser Leu Lys Glu Glu Thr Ile Lys Phe 
            180                 185                 190         
Ala Gly Lys Leu Met Glu Lys Ser Pro Ile Ala Leu Ala Ile Leu Lys 
        195                 200                 205             
His Ile Ile Leu Tyr Gly Asn Asp Ser Pro Leu Leu Asp Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Ala Phe Ser Thr Glu Asp Glu Lys 
225                 230                 235                 240 
Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Val Phe Lys Gly 
                245                 250                 255     
Lys 
    

<210> 15
<211> 429
<212> PRT
<213> Staphylococcus aureus C0637

<220> 
<223> Uncharacterized protein

<400> 15
Met Gly Lys Gln Ile Pro Lys Asp Arg Gly Leu Asp Ser Thr Leu Lys 
1               5                   10                  15      
Val Leu Lys Glu Gly Tyr Lys Tyr Val Pro Asn Arg Leu Glu Lys Phe 
            20                  25                  30          
Asp Thr Asn Ile Phe Glu Leu Arg Ala Leu Gly Gly Arg Arg Thr Val 
        35                  40                  45              
Val Phe Ser Gly Lys Glu Ala Ala Glu Ile Phe Tyr Asn Asn Glu Leu 
    50                  55                  60                  
Ile Glu Arg Gln Gly Thr Leu Pro Lys Arg Val Val Asn Thr Leu Phe 
65                  70                  75                  80  
Gly Lys Gly Ala Ile His Thr Thr Gly Gly Lys Lys His Ile Asp Arg 
                85                  90                  95      
Lys Ala Leu Phe Met Ser Leu Met Thr Glu Glu Asn Leu Glu Tyr Leu 
            100                 105                 110         
Arg Glu Leu Thr Arg Ser Thr Trp Phe Met Asn Thr Glu Arg Met Glu 
        115                 120                 125             
Arg Met Asp Glu Val Asn Val Tyr Lys Glu Ser Ile Ile Leu Leu Thr 
    130                 135                 140                 
Lys Val Gly Phe Arg Trp Ala Gly Ile Ile Ala Ser Pro Glu Glu Ile 
145                 150                 155                 160 
Glu Ser Cys Ala Lys Asp Met Asp Thr Met Ile Asp Ser Phe Lys Asn 
                165                 170                 175     
Ile Gly Thr Ala Phe Lys Gly Tyr Arg Glu Ala Lys Lys Ala Arg Asp 
            180                 185                 190         
Arg Val Glu Thr Phe Leu Glu Asn Gln Ile Ile Ala Val Arg Glu Gly 
        195                 200                 205             
Lys Leu Thr Pro Pro Gln Gly Thr Ala Leu His Glu Phe Ser His Trp 
    210                 215                 220                 
Glu Asp Phe Glu Gly Asn Leu Met Asp Ser Arg Leu Cys Ala Ile Asp 
225                 230                 235                 240 
Leu Met Asn Val Val Arg Pro Leu Val Ala Ile Asn Arg Phe Val Ser 
                245                 250                 255     
Phe Gly Val Lys Ala Leu His Asp Tyr Pro Gly Glu Ala Glu Lys Val 
            260                 265                 270         
Phe Asn Asn Glu Asn Asp Tyr Ala Tyr Lys Phe Val Gln Glu Val Arg 
        275                 280                 285             
Arg Phe Tyr Pro Phe Val Pro Phe Leu Pro Gly Lys Ala Ala Val Asp 
    290                 295                 300                 
Ile Glu Phe Asp Gly Tyr Thr Ile Glu Lys Asp Thr Phe Leu Val Leu 
305                 310                 315                 320 
Asp Ile Tyr Gly Thr Leu His Arg Glu Gly Leu Trp Glu Asn Pro Glu 
                325                 330                 335     
Arg Phe Tyr Pro Asn Arg Phe Ser Asp Trp Asp Gly Ser Pro Phe Asp 
            340                 345                 350         
Leu Ile Pro Gln Gly Gly Gly Asp Tyr Tyr Thr Asn His Arg Cys Ala 
        355                 360                 365             
Gly Glu Trp Met Thr Ile Ile Ile Met Glu Glu Ser Met Lys Tyr Phe 
    370                 375                 380                 
Ala Arg Asn Ile Ser Tyr Asp Met Lys Lys Asp Gln Asp Leu Ser Val 
385                 390                 395                 400 
Asn Leu Asn Lys Leu Pro Gly Arg Val Val Ser Gly Thr Ile Ile Glu 
                405                 410                 415     
Asn Val Asn Ala Leu Val Asn Arg Asn Val Glu Ser Val 
            420                 425                 

<210> 16
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..55
<223> /mol_type="unassigned DNA"
      /note="Primer oRH1 fwd"
      /organism="Artificial Sequence"

<400> 16
taattttgtt taactttaag aaggagatat acatatggct gattgggtaa caggc          55


<210> 17
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..52
<223> /mol_type="unassigned DNA"
      /note="Primer oRH2 Rev"
      /organism="Artificial Sequence"

<400> 17
catctcagta cctcctcatt ttgttgaatt cctattacca gtaatgctcc gc             52


<210> 18
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..53
<223> /mol_type="unassigned DNA"
      /note="Primer oRH3 Fwd"
      /organism="Artificial Sequence"

<400> 18
ataggaattc aacaaaatga ggaggtactg agatggcaac cctgaaacgt gaa            53


<210> 19
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..55
<223> /mol_type="unassigned DNA"
      /note="Primer oRH4 Rev"
      /organism="Artificial Sequence"

<400> 19
ggctagcccg tttgatctcg agtgcggccg cttattaggt gcgatccaca acttc          55


<210> 20
<211> 301
<212> PRT
<213> Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787

<220> 
<223> Phosphate butyryltransferase

<400> 20
Met Ile Lys Ser Phe Asn Glu Ile Ile Met Lys Val Lys Ser Lys Glu 
1               5                   10                  15      
Met Lys Lys Val Ala Val Ala Val Ala Gln Asp Glu Pro Val Leu Glu 
            20                  25                  30          
Ala Val Arg Asp Ala Lys Lys Asn Gly Ile Ala Asp Ala Ile Leu Val 
        35                  40                  45              
Gly Asp His Asp Glu Ile Val Ser Ile Ala Leu Lys Ile Gly Met Asp 
    50                  55                  60                  
Val Asn Asp Phe Glu Ile Val Asn Glu Pro Asn Val Lys Lys Ala Ala 
65                  70                  75                  80  
Leu Lys Ala Val Glu Leu Val Ser Thr Gly Lys Ala Asp Met Val Met 
                85                  90                  95      
Lys Gly Leu Val Asn Thr Ala Thr Phe Leu Arg Ser Val Leu Asn Lys 
            100                 105                 110         
Glu Val Gly Leu Arg Thr Gly Lys Thr Met Ser His Val Ala Val Phe 
        115                 120                 125             
Glu Thr Glu Lys Phe Asp Arg Leu Leu Phe Leu Thr Asp Val Ala Phe 
    130                 135                 140                 
Asn Thr Tyr Pro Glu Leu Lys Glu Lys Ile Asp Ile Val Asn Asn Ser 
145                 150                 155                 160 
Val Lys Val Ala His Ala Ile Gly Ile Glu Asn Pro Lys Val Ala Pro 
                165                 170                 175     
Ile Cys Ala Val Glu Val Ile Asn Pro Lys Met Pro Ser Thr Leu Asp 
            180                 185                 190         
Ala Ala Met Leu Ser Lys Met Ser Asp Arg Gly Gln Ile Lys Gly Cys 
        195                 200                 205             
Val Val Asp Gly Pro Leu Ala Leu Asp Ile Ala Leu Ser Glu Glu Ala 
    210                 215                 220                 
Ala His His Lys Gly Val Thr Gly Glu Val Ala Gly Lys Ala Asp Ile 
225                 230                 235                 240 
Phe Leu Met Pro Asn Ile Glu Thr Gly Asn Val Met Tyr Lys Thr Leu 
                245                 250                 255     
Thr Tyr Thr Thr Asp Ser Lys Asn Gly Gly Ile Leu Val Gly Thr Ser 
            260                 265                 270         
Ala Pro Val Val Leu Thr Ser Arg Ala Asp Ser His Glu Thr Lys Met 
        275                 280                 285             
Asn Ser Ile Ala Leu Ala Ala Leu Val Ala Gly Asn Lys 
    290                 295                 300     

<210> 21
<211> 329
<212> PRT
<213> Corynebacterium glutamicum ATCC 13032

<220> 
<223> Phosphate acetyltransferase

<400> 21
Met Ser Ala Glu Leu Phe Glu Asn Trp Leu Leu Lys Arg Ala Arg Ala 
1               5                   10                  15      
Glu His Ser His Ile Val Leu Pro Glu Gly Asp Asp Asp Arg Ile Leu 
            20                  25                  30          
Met Ala Ala His Gln Leu Leu Asp Gln Asp Ile Cys Asp Ile Thr Ile 
        35                  40                  45              
Leu Gly Asp Pro Val Lys Ile Lys Glu Arg Ala Thr Glu Leu Gly Leu 
    50                  55                  60                  
His Leu Asn Thr Ala Tyr Leu Val Asn Pro Leu Thr Asp Pro Arg Leu 
65                  70                  75                  80  
Glu Glu Phe Ala Glu Gln Phe Ala Glu Leu Arg Lys Ser Lys Ser Val 
                85                  90                  95      
Thr Ile Asp Glu Ala Arg Glu Ile Met Lys Asp Ile Ser Tyr Phe Gly 
            100                 105                 110         
Thr Met Met Val His Asn Gly Asp Ala Asp Gly Met Val Ser Gly Ala 
        115                 120                 125             
Ala Asn Thr Thr Ala His Thr Ile Lys Pro Ser Phe Gln Ile Ile Lys 
    130                 135                 140                 
Thr Val Pro Glu Ala Ser Val Val Ser Ser Ile Phe Leu Met Val Leu 
145                 150                 155                 160 
Arg Gly Arg Leu Trp Ala Phe Gly Asp Cys Ala Val Asn Pro Asn Pro 
                165                 170                 175     
Thr Ala Glu Gln Leu Gly Glu Ile Ala Val Val Ser Ala Lys Thr Ala 
            180                 185                 190         
Ala Gln Phe Gly Ile Asp Pro Arg Val Ala Ile Leu Ser Tyr Ser Thr 
        195                 200                 205             
Gly Asn Ser Gly Gly Gly Ser Asp Val Asp Arg Ala Ile Asp Ala Leu 
    210                 215                 220                 
Ala Glu Ala Arg Arg Leu Asn Pro Glu Leu Cys Val Asp Gly Pro Leu 
225                 230                 235                 240 
Gln Phe Asp Ala Ala Val Asp Pro Gly Val Ala Arg Lys Lys Met Pro 
                245                 250                 255     
Asp Ser Asp Val Ala Gly Gln Ala Asn Val Phe Ile Phe Pro Asp Leu 
            260                 265                 270         
Glu Ala Gly Asn Ile Gly Tyr Lys Thr Ala Gln Arg Thr Gly His Ala 
        275                 280                 285             
Leu Ala Val Gly Pro Ile Leu Gln Gly Leu Asn Lys Pro Val Asn Asp 
    290                 295                 300                 
Leu Ser Arg Gly Ala Thr Val Pro Asp Ile Val Asn Thr Val Ala Ile 
305                 310                 315                 320 
Thr Ala Ile Gln Ala Gly Gly Arg Ser 
                325                 

<210> 22
<211> 555
<212> PRT
<213> Klebsiella pneumoniae

<220> 
<223> Glycerol dehydratase alpha subunit

<400> 22
Met Lys Arg Ser Lys Arg Phe Ala Val Leu Ala Gln Arg Pro Val Asn 
1               5                   10                  15      
Gln Asp Gly Leu Ile Gly Glu Trp Pro Glu Glu Gly Leu Ile Ala Met 
            20                  25                  30          
Asp Ser Pro Phe Asp Pro Val Ser Ser Val Lys Val Asp Asn Gly Leu 
        35                  40                  45              
Ile Val Glu Leu Asp Gly Lys Arg Arg Asp Gln Phe Asp Met Ile Asp 
    50                  55                  60                  
Arg Phe Ile Ala Asp Tyr Ala Ile Asn Val Glu Arg Thr Glu Gln Ala 
65                  70                  75                  80  
Met Arg Leu Glu Ala Val Glu Ile Ala Arg Met Leu Val Asp Ile His 
                85                  90                  95      
Val Ser Arg Glu Glu Ile Ile Ala Ile Thr Thr Ala Ile Thr Pro Ala 
            100                 105                 110         
Lys Ala Val Glu Val Met Ala Gln Met Asn Val Val Glu Met Met Met 
        115                 120                 125             
Ala Leu Gln Lys Met Arg Ala Arg Arg Thr Pro Ser Asn Gln Cys His 
    130                 135                 140                 
Val Thr Asn Leu Lys Asp Asn Pro Val Gln Ile Ala Ala Asp Ala Ala 
145                 150                 155                 160 
Glu Ala Gly Ile Arg Gly Phe Ser Glu Gln Glu Thr Thr Val Gly Ile 
                165                 170                 175     
Ala Arg Tyr Ala Pro Phe Asn Ala Leu Ala Leu Leu Val Gly Ser Gln 
            180                 185                 190         
Cys Gly Arg Pro Gly Val Leu Thr Gln Cys Ser Val Glu Glu Ala Thr 
        195                 200                 205             
Glu Leu Glu Leu Gly Met Arg Gly Leu Thr Ser Tyr Ala Glu Thr Val 
    210                 215                 220                 
Ser Val Tyr Gly Thr Glu Ala Val Phe Thr Asp Gly Asp Asp Thr Pro 
225                 230                 235                 240 
Trp Ser Lys Ala Phe Leu Ala Ser Ala Tyr Ala Ser Arg Gly Leu Lys 
                245                 250                 255     
Met Arg Tyr Thr Ser Gly Thr Gly Ser Glu Ala Leu Met Gly Tyr Ser 
            260                 265                 270         
Glu Ser Lys Ser Met Leu Tyr Leu Glu Ser Arg Cys Ile Phe Ile Thr 
        275                 280                 285             
Lys Gly Ala Gly Val Gln Gly Leu Gln Asn Gly Ala Val Ser Cys Ile 
    290                 295                 300                 
Gly Met Thr Gly Ala Val Pro Ser Gly Ile Arg Ala Val Leu Ala Glu 
305                 310                 315                 320 
Asn Leu Ile Ala Ser Met Leu Asp Leu Glu Val Ala Ser Ala Asn Asp 
                325                 330                 335     
Gln Thr Phe Ser His Ser Asp Ile Arg Arg Thr Ala Arg Thr Leu Met 
            340                 345                 350         
Gln Met Leu Pro Gly Thr Asp Phe Ile Phe Ser Gly Tyr Ser Ala Val 
        355                 360                 365             
Pro Asn Tyr Asp Asn Met Phe Ala Gly Ser Asn Phe Asp Ala Glu Asp 
    370                 375                 380                 
Phe Asp Asp Tyr Asn Ile Leu Gln Arg Asp Leu Met Val Asp Gly Gly 
385                 390                 395                 400 
Leu Arg Pro Val Thr Glu Ala Glu Thr Ile Ala Ile Arg Gln Lys Ala 
                405                 410                 415     
Ala Arg Ala Ile Gln Ala Val Phe Arg Glu Leu Gly Leu Pro Pro Ile 
            420                 425                 430         
Ala Asp Glu Glu Val Glu Ala Ala Thr Tyr Ala His Gly Ser Asn Glu 
        435                 440                 445             
Met Pro Pro Arg Asn Val Val Glu Asp Leu Ser Ala Val Glu Glu Met 
    450                 455                 460                 
Met Lys Arg Asn Ile Thr Gly Leu Asp Ile Val Gly Ala Leu Ser Arg 
465                 470                 475                 480 
Ser Gly Phe Glu Asp Ile Ala Ser Asn Ile Leu Asn Met Leu Arg Gln 
                485                 490                 495     
Arg Val Thr Gly Asp Tyr Leu Gln Thr Ser Ala Ile Leu Asp Arg Gln 
            500                 505                 510         
Phe Glu Val Val Ser Ala Val Asn Asp Ile Asn Asp Tyr Gln Gly Pro 
        515                 520                 525             
Gly Thr Gly Tyr Arg Ile Ser Ala Glu Arg Trp Ala Glu Ile Lys Asn 
    530                 535                 540                 
Ile Pro Gly Val Val Gln Pro Asp Thr Ile Glu 
545                 550                 555 

<210> 23
<211> 146
<212> PRT
<213> Klebsiella pneumoniae

<220> 
<223> Glycerol dehydratase medium subunit

<400> 23
Met Pro His Gly Ala Ile Leu Lys Glu Leu Ile Ala Gly Val Glu Glu 
1               5                   10                  15      
Glu Gly Leu His Ala Arg Val Val Arg Ile Leu Arg Thr Ser Asp Val 
            20                  25                  30          
Ser Phe Met Ala Trp Asp Ala Ala Asn Leu Ser Gly Ser Gly Ile Gly 
        35                  40                  45              
Ile Gly Ile Gln Ser Lys Gly Thr Thr Val Ile His Gln Arg Asp Leu 
    50                  55                  60                  
Leu Pro Leu Ser Asn Leu Glu Leu Phe Ser Gln Ala Pro Leu Leu Thr 
65                  70                  75                  80  
Leu Glu Thr Tyr Arg Gln Ile Gly Lys Asn Ala Ala Arg Tyr Ala Arg 
                85                  90                  95      
Lys Glu Ser Pro Ser Pro Val Pro Val Val Asn Asp Gln Met Val Arg 
            100                 105                 110         
Pro Lys Phe Met Ala Lys Ala Ala Leu Phe His Ile Lys Glu Thr Lys 
        115                 120                 125             
His Val Val Gln Asp Ala Glu Pro Val Thr Leu His Val Asp Leu Val 
    130                 135                 140                 
Arg Glu 
145     

<210> 24
<211> 141
<212> PRT
<213> Klebsiella pneumoniae

<220> 
<223> Glycerol dehydratase gamma subunit

<400> 24
Met Ser Glu Lys Thr Met Arg Val Gln Asp Tyr Pro Leu Ala Thr Arg 
1               5                   10                  15      
Cys Pro Glu His Ile Leu Thr Pro Thr Gly Lys Pro Leu Thr Asp Ile 
            20                  25                  30          
Thr Leu Glu Lys Val Leu Ser Gly Glu Val Gly Pro Gln Asp Val Arg 
        35                  40                  45              
Ile Ser Arg Gln Thr Leu Glu Tyr Gln Ala Gln Ile Ala Glu Gln Met 
    50                  55                  60                  
Gln Arg His Ala Val Ala Arg Asn Phe Arg Arg Ala Ala Glu Leu Ile 
65                  70                  75                  80  
Ala Ile Pro Asp Glu Arg Ile Leu Ala Ile Tyr Asn Ala Leu Arg Pro 
                85                  90                  95      
Phe Arg Ser Ser Gln Ala Glu Leu Leu Ala Ile Ala Asp Glu Leu Glu 
            100                 105                 110         
His Thr Trp His Ala Thr Val Asn Ala Ala Phe Val Arg Glu Ser Ala 
        115                 120                 125             
Glu Val Tyr Gln Gln Arg His Lys Leu Arg Lys Gly Ser 
    130                 135                 140     

<210> 25
<211> 477
<212> PRT
<213> Lactobacillus reuteri JCM 1112


<400> 25
Met Gln Ile Asn Asp Ile Glu Ser Ala Val Arg Lys Ile Leu Ala Glu 
1               5                   10                  15      
Glu Leu Asp Asn Ala Ser Ser Ser Ser Ala Asn Val Ala Ala Thr Thr 
            20                  25                  30          
Asp Asn Gly His Arg Gly Ile Phe Thr Asn Val Asn Asp Ala Ile Ala 
        35                  40                  45              
Ala Ala Lys Ala Ala Gln Glu Ile Tyr Arg Asp Lys Pro Ile Ala Val 
    50                  55                  60                  
Arg Gln Gln Val Ile Asp Ala Ile Lys Glu Gly Phe Arg Pro Tyr Ile 
65                  70                  75                  80  
Glu Lys Met Ala Lys Asp Ile Lys Glu Glu Thr Gly Met Gly Thr Val 
                85                  90                  95      
Glu Ala Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly 
            100                 105                 110         
Pro Glu Ile Leu Glu Pro Val Val Glu Asn Gly Asp Gly Gly Met Val 
        115                 120                 125             
Met Tyr Glu Arg Leu Pro Tyr Gly Val Ile Gly Ala Val Gly Pro Ser 
    130                 135                 140                 
Thr Asn Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala 
145                 150                 155                 160 
Gly Gly Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Asn Val 
                165                 170                 175     
Thr Arg Trp Thr Ile Glu Lys Met Asn Asp Phe Ile Ala Asp Ala Thr 
            180                 185                 190         
Gly Leu His Asn Leu Val Val Ser Ile Glu Thr Pro Thr Ile Glu Ser 
        195                 200                 205             
Val Gln Gln Met Met Lys His Pro Asp Ile Ala Met Leu Ala Val Thr 
    210                 215                 220                 
Gly Gly Pro Ala Val Val His Gln Ala Met Thr Ser Gly Lys Lys Ala 
225                 230                 235                 240 
Val Gly Ala Gly Pro Gly Asn Pro Pro Ala Met Val Asp Ala Thr Ala 
                245                 250                 255     
Asp Ile Asp Leu Ala Ala His Asn Ile Ile Thr Ser Ala Ser Phe Asp 
            260                 265                 270         
Asn Asp Ile Leu Cys Thr Ala Glu Lys Glu Val Val Ala Glu Ser Ser 
        275                 280                 285             
Ile Lys Asp Glu Leu Ile Arg Lys Met Gln Asp Glu Gly Ala Phe Val 
    290                 295                 300                 
Val Asn Arg Glu Gln Ala Asp Lys Leu Ala Asp Met Cys Ile Gln Glu 
305                 310                 315                 320 
Asn Gly Ala Pro Asp Arg Lys Phe Val Gly Lys Asp Ala Thr Tyr Ile 
                325                 330                 335     
Leu Asp Gln Ala Asn Ile Pro Tyr Thr Gly His Pro Val Glu Ile Ile 
            340                 345                 350         
Cys Glu Leu Pro Lys Glu His Pro Leu Val Met Thr Glu Met Leu Met 
        355                 360                 365             
Pro Ile Leu Pro Val Val Ser Cys Pro Thr Phe Asp Asp Val Leu Lys 
    370                 375                 380                 
Thr Ala Val Glu Val Glu Lys Gly Asn His His Thr Ala Thr Ile His 
385                 390                 395                 400 
Ser Asn Asn Leu Lys His Ile Asn Asn Ala Ala His Arg Met Gln Cys 
                405                 410                 415     
Ser Ile Phe Val Val Asn Gly Pro Ser Tyr Val Gly Thr Gly Val Ala 
            420                 425                 430         
Asp Asn Gly Ala His Ser Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro 
        435                 440                 445             
Thr Gly Glu Gly Thr Cys Thr Ala Arg Thr Phe Thr Arg Arg Val Arg 
    450                 455                 460                 
Leu Asn Ser Pro Gln Gly Phe Ser Val Arg Asn Trp Tyr 
465                 470                 475         

<210> 26
<211> 332
<212> PRT
<213> Metallosphaera sedula (strain ATCC 51363 /DSM 5348)


<400> 26
Met Lys Ala Val Val Val Lys Gly His Lys Gln Gly Tyr Glu Val Arg 
1               5                   10                  15      
Glu Val Gln Asp Pro Lys Pro Ala Ser Gly Glu Val Ile Ile Lys Val 
            20                  25                  30          
Arg Arg Ala Ala Leu Cys Tyr Arg Asp Leu Leu Gln Leu Gln Gly Phe 
        35                  40                  45              
Tyr Pro Arg Met Lys Tyr Pro Val Val Leu Gly His Glu Val Val Gly 
    50                  55                  60                  
Glu Ile Leu Glu Val Gly Glu Gly Val Thr Gly Phe Ser Pro Gly Asp 
65                  70                  75                  80  
Arg Val Ile Ser Leu Leu Tyr Ala Pro Asp Gly Thr Cys His Tyr Cys 
                85                  90                  95      
Arg Gln Gly Glu Glu Ala Tyr Cys His Ser Arg Leu Gly Tyr Ser Glu 
            100                 105                 110         
Glu Leu Asp Gly Phe Phe Ser Glu Met Ala Lys Val Lys Val Thr Ser 
        115                 120                 125             
Leu Val Lys Val Pro Thr Arg Ala Ser Asp Glu Gly Ala Val Met Val 
    130                 135                 140                 
Pro Cys Val Thr Gly Met Val Tyr Arg Gly Leu Arg Arg Ala Asn Leu 
145                 150                 155                 160 
Arg Glu Gly Glu Thr Val Leu Val Thr Gly Ala Ser Gly Gly Val Gly 
                165                 170                 175     
Ile His Ala Leu Gln Val Ala Lys Ala Met Gly Ala Arg Val Val Gly 
            180                 185                 190         
Val Thr Thr Ser Glu Glu Lys Ala Ser Ile Val Gly Lys Tyr Ala Asp 
        195                 200                 205             
Arg Val Ile Val Gly Ser Lys Phe Ser Glu Glu Ala Lys Lys Glu Asp 
    210                 215                 220                 
Ile Asn Val Val Ile Asp Thr Val Gly Thr Pro Thr Phe Asp Glu Ser 
225                 230                 235                 240 
Leu Lys Ser Leu Trp Met Gly Gly Arg Ile Val Gln Ile Gly Asn Val 
                245                 250                 255     
Asp Pro Thr Gln Ser Tyr Gln Leu Arg Leu Gly Tyr Thr Ile Leu Lys 
            260                 265                 270         
Asp Ile Ala Ile Ile Gly His Ala Ser Ala Thr Arg Arg Asp Ala Glu 
        275                 280                 285             
Gly Ala Leu Lys Leu Thr Ala Glu Gly Lys Ile Arg Pro Val Val Ala 
    290                 295                 300                 
Gly Thr Val His Leu Glu Glu Ile Asp Lys Gly Tyr Glu Met Leu Lys 
305                 310                 315                 320 
Asp Lys His Lys Val Gly Lys Val Leu Leu Thr Thr 
                325                 330         

<210> 27
<211> 402
<212> PRT
<213> Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

<220> 
<223> Salty propionate kinase

<400> 27
Met Asn Glu Phe Pro Val Val Leu Val Ile Asn Cys Gly Ser Ser Ser 
1               5                   10                  15      
Ile Lys Phe Ser Val Leu Asp Val Ala Thr Cys Asp Val Leu Met Ala 
            20                  25                  30          
Gly Ile Ala Asp Gly Met Asn Thr Glu Asn Ala Phe Leu Ser Ile Asn 
        35                  40                  45              
Gly Asp Lys Pro Ile Asn Leu Ala His Ser Asn Tyr Glu Asp Ala Leu 
    50                  55                  60                  
Lys Ala Ile Ala Phe Glu Leu Glu Lys Arg Asp Leu Thr Asp Ser Val 
65                  70                  75                  80  
Ala Leu Ile Gly His Arg Ile Ala His Gly Gly Glu Leu Phe Thr Gln 
                85                  90                  95      
Ser Val Ile Ile Thr Asp Glu Ile Ile Asp Asn Ile Arg Arg Val Ser 
            100                 105                 110         
Pro Leu Ala Pro Leu His Asn Tyr Ala Asn Leu Ser Gly Ile Asp Ala 
        115                 120                 125             
Ala Arg His Leu Phe Pro Ala Val Arg Gln Val Ala Val Phe Asp Thr 
    130                 135                 140                 
Ser Phe His Gln Thr Leu Ala Pro Glu Ala Tyr Leu Tyr Gly Leu Pro 
145                 150                 155                 160 
Trp Glu Tyr Phe Ser Ser Leu Gly Val Arg Arg Tyr Gly Phe His Gly 
                165                 170                 175     
Thr Ser His Arg Tyr Val Ser Arg Arg Ala Tyr Glu Leu Leu Asp Leu 
            180                 185                 190         
Asp Glu Lys Asp Ser Gly Leu Ile Val Ala His Leu Gly Asn Gly Ala 
        195                 200                 205             
Ser Ile Cys Ala Val Arg Asn Gly Gln Ser Val Asp Thr Ser Met Gly 
    210                 215                 220                 
Met Thr Pro Leu Glu Gly Leu Met Met Gly Thr Arg Ser Gly Asp Val 
225                 230                 235                 240 
Asp Phe Gly Ala Met Ala Trp Ile Ala Lys Glu Thr Gly Gln Thr Leu 
                245                 250                 255     
Ser Asp Leu Glu Arg Val Val Asn Lys Glu Ser Gly Leu Leu Gly Ile 
            260                 265                 270         
Ser Gly Leu Ser Ser Asp Leu Arg Val Leu Glu Lys Ala Trp His Glu 
        275                 280                 285             
Gly His Glu Arg Ala Arg Leu Ala Ile Lys Thr Phe Val His Arg Ile 
    290                 295                 300                 
Ala Arg His Ile Ala Gly His Ala Ala Ser Leu His Arg Leu Asp Gly 
305                 310                 315                 320 
Ile Ile Phe Thr Gly Gly Ile Gly Glu Asn Ser Val Leu Ile Arg Gln 
                325                 330                 335     
Leu Val Ile Glu His Leu Gly Val Leu Gly Leu Thr Leu Asp Val Glu 
            340                 345                 350         
Met Asn Lys Gln Pro Asn Ser His Gly Glu Arg Ile Ile Ser Ala Asn 
        355                 360                 365             
Pro Ser Gln Val Ile Cys Ala Val Ile Pro Thr Asn Glu Glu Lys Met 
    370                 375                 380                 
Ile Ala Leu Asp Ala Ile His Leu Gly Asn Val Lys Ala Pro Val Glu 
385                 390                 395                 400 
Phe Ala 
        

<210> 28
<211> 402
<212> PRT
<213> Escherichia coli (strain K12) 

<220> 
<223> Propionate kinase

<400> 28
Met Asn Glu Phe Pro Val Val Leu Val Ile Asn Cys Gly Ser Ser Ser 
1               5                   10                  15      
Ile Lys Phe Ser Val Leu Asp Ala Ser Asp Cys Glu Val Leu Met Ser 
            20                  25                  30          
Gly Ile Ala Asp Gly Ile Asn Ser Glu Asn Ala Phe Leu Ser Val Asn 
        35                  40                  45              
Gly Gly Glu Pro Ala Pro Leu Ala His His Ser Tyr Glu Gly Ala Leu 
    50                  55                  60                  
Lys Ala Ile Ala Phe Glu Leu Glu Lys Arg Asn Leu Asn Asp Ser Val 
65                  70                  75                  80  
Ala Leu Ile Gly His Arg Ile Ala His Gly Gly Ser Ile Phe Thr Glu 
                85                  90                  95      
Ser Ala Ile Ile Thr Asp Glu Val Ile Asp Asn Ile Arg Arg Val Ser 
            100                 105                 110         
Pro Leu Ala Pro Leu His Asn Tyr Ala Asn Leu Ser Gly Ile Glu Ser 
        115                 120                 125             
Ala Gln Gln Leu Phe Pro Gly Val Thr Gln Val Ala Val Phe Asp Thr 
    130                 135                 140                 
Ser Phe His Gln Thr Met Ala Pro Glu Ala Tyr Leu Tyr Gly Leu Pro 
145                 150                 155                 160 
Trp Lys Tyr Tyr Glu Glu Leu Gly Val Arg Arg Tyr Gly Phe His Gly 
                165                 170                 175     
Thr Ser His Arg Tyr Val Ser Gln Arg Ala His Ser Leu Leu Asn Leu 
            180                 185                 190         
Ala Glu Asp Asp Ser Gly Leu Val Val Ala His Leu Gly Asn Gly Ala 
        195                 200                 205             
Ser Ile Cys Ala Val Arg Asn Gly Gln Ser Val Asp Thr Ser Met Gly 
    210                 215                 220                 
Met Thr Pro Leu Glu Gly Leu Met Met Gly Thr Arg Ser Gly Asp Val 
225                 230                 235                 240 
Asp Phe Gly Ala Met Ser Trp Val Ala Ser Gln Thr Asn Gln Ser Leu 
                245                 250                 255     
Gly Asp Leu Glu Arg Val Val Asn Lys Glu Ser Gly Leu Leu Gly Ile 
            260                 265                 270         
Ser Gly Leu Ser Ser Asp Leu Arg Val Leu Glu Lys Ala Trp His Glu 
        275                 280                 285             
Gly His Glu Arg Ala Gln Leu Ala Ile Lys Thr Phe Val His Arg Ile 
    290                 295                 300                 
Ala Arg His Ile Ala Gly His Ala Ala Ser Leu Arg Arg Leu Asp Gly 
305                 310                 315                 320 
Ile Ile Phe Thr Gly Gly Ile Gly Glu Asn Ser Ser Leu Ile Arg Arg 
                325                 330                 335     
Leu Val Met Glu His Leu Ala Val Leu Gly Leu Glu Ile Asp Thr Glu 
            340                 345                 350         
Met Asn Asn Arg Ser Asn Ser Cys Gly Glu Arg Ile Val Ser Ser Glu 
        355                 360                 365             
Asn Ala Arg Val Ile Cys Ala Val Ile Pro Thr Asn Glu Glu Lys Met 
    370                 375                 380                 
Ile Ala Leu Asp Ala Ile His Leu Gly Lys Val Asn Ala Pro Ala Glu 
385                 390                 395                 400 
Phe Ala 
        

<210> 29
<211> 260
<212> PRT
<213> Pseudomonas aeruginosa (strain UCBPP-PA14)

<220> 
<223> Uncharacterized protein

<400> 29
Met Ser Glu Phe Phe Asp Arg Thr Gly Pro Leu Gln Glu Ala Gly Ser 
1               5                   10                  15      
Tyr Pro Gln Trp Ala Gln Gln Leu Ile Val Asp Cys Gln Ala Ser Lys 
            20                  25                  30          
Asp Arg Val Ser Gly His Glu Leu Tyr Arg Arg Met Arg Asp Ala Glu 
        35                  40                  45              
Leu Ser Pro Ala Leu Met Arg Leu Tyr Leu Ile Gly Gly Trp Pro Val 
    50                  55                  60                  
Val Glu Gln Phe Pro Leu Tyr Met Ser Gln Asn Leu Leu Lys Thr Arg 
65                  70                  75                  80  
Phe Ala Arg His Pro Gly Glu Asp Met Ala Arg Arg Trp Leu Met Arg 
                85                  90                  95      
Asn Ile Arg Val Glu Leu Asn His Ala Asp Tyr Trp Leu His Trp Ala 
            100                 105                 110         
Glu Ala His Gly Val Ser Leu Ala Glu Ile Gln Ala Gln Asp Val Pro 
        115                 120                 125             
Ala Glu Leu His Ala Leu Ser His Trp Cys Trp His Thr Cys Ala Ser 
    130                 135                 140                 
Asp Ser Leu Pro Val Ala Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly 
145                 150                 155                 160 
Val Thr Gly Glu Trp Ser Ala Leu Val Cys Ser Asn Gly Val Tyr Glu 
                165                 170                 175     
Asn Ala Phe Pro Lys Glu Gly Arg Lys Arg Ala Met Lys Trp Leu Lys 
            180                 185                 190         
Leu His Ala Gln Tyr Asp Asp Ala His Pro Trp Glu Ala Leu Glu Ile 
        195                 200                 205             
Ile Cys Thr Leu Ala Gly Thr Asn Pro Ser Ala Glu Leu Arg Arg Gln 
    210                 215                 220                 
Leu Arg Asp Ala Ile Cys Lys Ser Tyr Asp Tyr Met Tyr Leu Phe Leu 
225                 230                 235                 240 
Glu Arg Cys Met Gln Leu Glu Glu Ala Arg Ser Ala Arg Lys Leu Ala 
                245                 250                 255     
Val Gly Ala Glu 
            260 

<210> 30
<211> 261
<212> PRT
<213> Pseudomonas syringae pv. tomato (strain DC3000)

<220> 
<223> Uncharacterized protein

<400> 30
Met Ile Asp Thr Phe Glu Arg Thr Gly Pro Leu Met Glu Ala Ser Ser 
1               5                   10                  15      
Tyr Pro Ala Trp Ala Gln Gln Leu Ile Asn Asp Cys Ser Pro Ala Lys 
            20                  25                  30          
Ala Arg Val Val Glu His Glu Leu Tyr Gln Gln Met Arg Asp Ala Lys 
        35                  40                  45              
Leu Ser Pro Gln Ile Met Arg Gln Tyr Leu Ile Gly Gly Trp Pro Val 
    50                  55                  60                  
Val Glu Gln Phe Ala Val Tyr Met Ala Lys Asn Leu Thr Lys Thr Arg 
65                  70                  75                  80  
Phe Gly Arg His Pro Gly Glu Asp Met Ala Arg Arg Trp Leu Met Arg 
                85                  90                  95      
Asn Ile Arg Val Glu Leu Asn His Ala Asp Tyr Trp Val Asn Trp Cys 
            100                 105                 110         
Ala Ala His Asp Val Thr Leu Glu Asp Leu His Asp Gln Arg Val Ala 
        115                 120                 125             
Pro Glu Leu His Ala Leu Ser His Trp Cys Trp Gln Thr Ser Ser Ser 
    130                 135                 140                 
Asp Ser Leu Ala Val Ala Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly 
145                 150                 155                 160 
Ala Thr Gly Glu Trp Ser Ala Val Val Cys Ser Thr Gly Val Tyr Ala 
                165                 170                 175     
Glu Ala Phe Ala Glu Glu Thr Arg Lys Lys Ser Met Lys Trp Leu Lys 
            180                 185                 190         
Met His Ala Gln Tyr Asp Asp Ala His Pro Trp Glu Ala Leu Glu Ile 
        195                 200                 205             
Ile Cys Thr Leu Val Gly Asn Lys Pro Ser Leu Gln Leu Gln Ala Glu 
    210                 215                 220                 
Leu Arg Gln Ala Val Thr Lys Ser Tyr Asp Tyr Met Tyr Leu Phe Leu 
225                 230                 235                 240 
Glu Arg Cys Ile Gln Leu Asp Lys Val Lys Ser Pro Arg Gly Arg Val 
                245                 250                 255     
Ala Ala Leu Glu Met 
            260     

<210> 31
<211> 270
<212> PRT
<213> OS=Pseudomonas putida (strain F1 / ATCC 700007)

<220> 
<223> Pyrroloquinoline quinone (Coenzyme PQQ) biosynthesis protein

<400> 31
Met Glu Ile Thr Arg Ile Lys Glu Leu Lys Val Ile Asp Ala Phe Val 
1               5                   10                  15      
Arg Ile Gly Pro Leu Met Asp Pro Ala Ser Tyr Pro Gln Trp Ala Gln 
            20                  25                  30          
Gln Leu Ile Glu Asp Cys Arg Glu Ser Lys Arg Arg Val Val Glu His 
        35                  40                  45              
Glu Phe Tyr Ala Arg Leu Arg Asp Gly Gln Leu Lys Gln Ser Thr Ile 
    50                  55                  60                  
Arg Gln Tyr Leu Ile Gly Gly Trp Pro Val Val Glu Gln Phe Ser Leu 
65                  70                  75                  80  
Tyr Met Ala His Asn Leu Thr Lys Thr Arg Tyr Gly Arg His Gln Gly 
                85                  90                  95      
Glu Asp Met Ala Arg Arg Trp Leu Met Arg Asn Ile Arg Val Glu Leu 
            100                 105                 110         
Asn His Ala Asp Tyr Trp Val Asn Trp Cys Gln Ala His Gly Val His 
        115                 120                 125             
Leu His Glu Leu Gln Ala Gln Glu Val Pro Pro Glu Leu Asn Gly Leu 
    130                 135                 140                 
Asn Asp Trp Cys Trp Arg Val Cys Ala Thr Glu Asn Leu Ala Ile Ser 
145                 150                 155                 160 
Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly Ala Thr Gly Glu Trp Ser 
                165                 170                 175     
Ala Val Val Cys Ser Thr Asp Thr Tyr Ala Gln Gly Phe Pro Glu Glu 
            180                 185                 190         
Gly Arg Lys Arg Ala Met Lys Trp Leu Lys Met His Ala Gln Tyr Asp 
        195                 200                 205             
Asp Ala His Pro Trp Glu Ala Leu Glu Ile Ile Cys Thr Leu Ala Gly 
    210                 215                 220                 
Glu Asn Pro Thr Leu Gly Leu Arg Thr Glu Leu Arg Arg Ala Ile Cys 
225                 230                 235                 240 
Lys Ser Tyr Asp Cys Met Phe Leu Phe Leu Glu Arg Cys Met Gln Leu 
                245                 250                 255     
Glu Gly Arg Gln Gln Gly Arg Met Arg Pro Ala Leu Ala Ala 
            260                 265                 270 

<210> 32
<211> 394
<212> PRT
<213> Cupriavidus necator

<220> 
<223> Cupriavidus necator ATCC 17699

<220> 
<223> beta-ketothiolase BktB

<400> 32
Met Thr Arg Glu Val Val Val Val Ser Gly Val Arg Thr Ala Ile Gly 
1               5                   10                  15      
Thr Phe Gly Gly Ser Leu Lys Asp Val Ala Pro Ala Glu Leu Gly Ala 
            20                  25                  30          
Leu Val Val Arg Glu Ala Leu Ala Arg Ala Gln Val Ser Gly Asp Asp 
        35                  40                  45              
Val Gly His Val Val Phe Gly Asn Val Ile Gln Thr Glu Pro Arg Asp 
    50                  55                  60                  
Met Tyr Leu Gly Arg Val Ala Ala Val Asn Gly Gly Val Thr Ile Asn 
65                  70                  75                  80  
Ala Pro Ala Leu Thr Val Asn Arg Leu Cys Gly Ser Gly Leu Gln Ala 
                85                  90                  95      
Ile Val Ser Ala Ala Gln Thr Ile Leu Leu Gly Asp Thr Asp Val Ala 
            100                 105                 110         
Ile Gly Gly Gly Ala Glu Ser Met Ser Arg Ala Pro Tyr Leu Ala Pro 
        115                 120                 125             
Ala Ala Arg Trp Gly Ala Arg Met Gly Asp Ala Gly Leu Val Asp Met 
    130                 135                 140                 
Met Leu Gly Ala Leu His Asp Pro Phe His Arg Ile His Met Gly Val 
145                 150                 155                 160 
Thr Ala Glu Asn Val Ala Lys Glu Tyr Asp Ile Ser Arg Ala Gln Gln 
                165                 170                 175     
Asp Glu Ala Ala Leu Glu Ser His Arg Arg Ala Ser Ala Ala Ile Lys 
            180                 185                 190         
Ala Gly Tyr Phe Lys Asp Gln Ile Val Pro Val Val Ser Lys Gly Arg 
        195                 200                 205             
Lys Gly Asp Val Thr Phe Asp Thr Asp Glu His Val Arg His Asp Ala 
    210                 215                 220                 
Thr Ile Asp Asp Met Thr Lys Leu Arg Pro Val Phe Val Lys Glu Asn 
225                 230                 235                 240 
Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Ala Ala Ala 
                245                 250                 255     
Ala Val Val Met Met Glu Arg Ala Glu Ala Glu Arg Arg Gly Leu Lys 
            260                 265                 270         
Pro Leu Ala Arg Leu Val Ser Tyr Gly His Ala Gly Val Asp Pro Lys 
        275                 280                 285             
Ala Met Gly Ile Gly Pro Val Pro Ala Thr Lys Ile Ala Leu Glu Arg 
    290                 295                 300                 
Ala Gly Leu Gln Val Ser Asp Leu Asp Val Ile Glu Ala Asn Glu Ala 
305                 310                 315                 320 
Phe Ala Ala Gln Ala Cys Ala Val Thr Lys Ala Leu Gly Leu Asp Pro 
                325                 330                 335     
Ala Lys Val Asn Pro Asn Gly Ser Gly Ile Ser Leu Gly His Pro Ile 
            340                 345                 350         
Gly Ala Thr Gly Ala Leu Ile Thr Val Lys Ala Leu His Glu Leu Asn 
        355                 360                 365             
Arg Val Gln Gly Arg Tyr Ala Leu Val Thr Met Cys Ile Gly Gly Gly 
    370                 375                 380                 
Gln Gly Ile Ala Ala Ile Phe Glu Arg Ile 
385                 390                 

<210> 33
<211> 282
<212> PRT
<213> Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787

<220> 
<223> 3-hydroxybutyryl-CoA dehydrogenase

<400> 33
Met Lys Lys Val Cys Val Ile Gly Ala Gly Thr Met Gly Ser Gly Ile 
1               5                   10                  15      
Ala Gln Ala Phe Ala Ala Lys Gly Phe Glu Val Val Leu Arg Asp Ile 
            20                  25                  30          
Lys Asp Glu Phe Val Asp Arg Gly Leu Asp Phe Ile Asn Lys Asn Leu 
        35                  40                  45              
Ser Lys Leu Val Lys Lys Gly Lys Ile Glu Glu Ala Thr Lys Val Glu 
    50                  55                  60                  
Ile Leu Thr Arg Ile Ser Gly Thr Val Asp Leu Asn Met Ala Ala Asp 
65                  70                  75                  80  
Cys Asp Leu Val Ile Glu Ala Ala Val Glu Arg Met Asp Ile Lys Lys 
                85                  90                  95      
Gln Ile Phe Ala Asp Leu Asp Asn Ile Cys Lys Pro Glu Thr Ile Leu 
            100                 105                 110         
Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr Glu Val Ala Ser Ala Thr 
        115                 120                 125             
Lys Arg Pro Asp Lys Val Ile Gly Met His Phe Phe Asn Pro Ala Pro 
    130                 135                 140                 
Val Met Lys Leu Val Glu Val Ile Arg Gly Ile Ala Thr Ser Gln Glu 
145                 150                 155                 160 
Thr Phe Asp Ala Val Lys Glu Thr Ser Ile Ala Ile Gly Lys Asp Pro 
                165                 170                 175     
Val Glu Val Ala Glu Ala Pro Gly Phe Val Val Asn Arg Ile Leu Ile 
            180                 185                 190         
Pro Met Ile Asn Glu Ala Val Gly Ile Leu Ala Glu Gly Ile Ala Ser 
        195                 200                 205             
Val Glu Asp Ile Asp Lys Ala Met Lys Leu Gly Ala Asn His Pro Met 
    210                 215                 220                 
Gly Pro Leu Glu Leu Gly Asp Phe Ile Gly Leu Asp Ile Cys Leu Ala 
225                 230                 235                 240 
Ile Met Asp Val Leu Tyr Ser Glu Thr Gly Asp Ser Lys Tyr Arg Pro 
                245                 250                 255     
His Thr Leu Leu Lys Lys Tyr Val Arg Ala Gly Trp Leu Gly Arg Lys 
            260                 265                 270         
Ser Gly Lys Gly Phe Tyr Asp Tyr Ser Lys 
        275                 280         

<210> 34
<211> 259
<212> PRT
<213> Metallosphaera cuprina Ar-4

<220> 
<223> 3-hydroxypropionyl-CoA dehydratase

<400> 34
Met Glu Tyr Glu Thr Leu Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp 
1               5                   10                  15      
Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ser Lys Leu 
            20                  25                  30          
Leu Glu Glu Leu Asn Arg Ala Val Ser Glu Gly Glu Ser Asp Pro Glu 
        35                  40                  45              
Val Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly 
    50                  55                  60                  
Ala Asp Ile Thr Gln Phe Asn Gln Leu Ser Pro Thr Asp Ala Trp Arg 
65                  70                  75                  80  
Phe Ser Lys Arg Gly Arg Glu Val Met Asp Lys Ile Glu Ser Leu Ser 
                85                  90                  95      
Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu 
            100                 105                 110         
Glu Leu Ala Leu Ser Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln 
        115                 120                 125             
Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly 
    130                 135                 140                 
Thr Gln Arg Leu Thr Arg Ile Val Gly Lys Gly Arg Ala Leu Glu Ile 
145                 150                 155                 160 
Met Met Thr Gly Asp Arg Leu Ser Gly Lys Asp Ala Glu Arg Tyr Gly 
                165                 170                 175     
Leu Val Asn Arg Val Thr Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg 
            180                 185                 190         
Lys Leu Ala Glu Lys Ile Ala Arg Lys Ser Pro Val Ser Leu Ala Leu 
        195                 200                 205             
Ile Lys Glu Val Val Asn Lys Gly Leu Asp Ser Pro Leu Ala Ser Gly 
    210                 215                 220                 
Leu Ser Leu Glu Ser Ile Gly Trp Gly Val Ile Phe Ser Thr Glu Asp 
225                 230                 235                 240 
Lys Lys Glu Gly Val Asn Ala Phe Leu Glu Lys Arg Glu Pro Asn Phe 
                245                 250                 255     
Lys Gly Lys 
            

<210> 35
<211> 259
<212> PRT
<213> Metallosphaera sedula

<220> 
<223> 3-hydroxypropionyl-coenzyme A dehydratase

<400> 35
Met Glu Phe Glu Thr Ile Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp 
1               5                   10                  15      
Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ala Lys Leu 
            20                  25                  30          
Leu Glu Glu Leu Asp Arg Ala Val Ser Gln Ala Glu Ser Asp Pro Glu 
        35                  40                  45              
Ile Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly 
    50                  55                  60                  
Ala Asp Ile Thr Gln Phe Asn Gln Leu Thr Pro Ala Glu Ala Trp Lys 
65                  70                  75                  80  
Phe Ser Lys Lys Gly Arg Glu Ile Met Asp Lys Ile Glu Ala Leu Ser 
                85                  90                  95      
Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu 
            100                 105                 110         
Glu Leu Ala Leu Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln 
        115                 120                 125             
Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly 
    130                 135                 140                 
Thr Gln Arg Leu Thr Arg Val Ile Gly Lys Gly Arg Ala Leu Glu Met 
145                 150                 155                 160 
Met Met Thr Gly Asp Arg Ile Pro Gly Lys Asp Ala Glu Lys Tyr Gly 
                165                 170                 175     
Leu Val Asn Arg Val Val Pro Leu Ala Asn Leu Glu Gln Glu Thr Arg 
            180                 185                 190         
Lys Leu Ala Glu Lys Ile Ala Lys Lys Ser Pro Ile Ser Leu Ala Leu 
        195                 200                 205             
Ile Lys Glu Val Val Asn Arg Gly Leu Asp Ser Pro Leu Leu Ser Gly 
    210                 215                 220                 
Leu Ala Leu Glu Ser Val Gly Trp Gly Val Val Phe Ser Thr Glu Asp 
225                 230                 235                 240 
Lys Lys Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Glu Pro Thr Phe 
                245                 250                 255     
Lys Gly Lys 
            

<210> 36
<211> 257
<212> PRT
<213> Sulfolobus tokodaii str. 7

<220> 
<223> 3-hydroxypropionyl-CoA dehydratase

<400> 36
Met Glu Thr Ile Val Ile Lys Lys Glu Thr Pro Ile Gly Trp Ile Tyr 
1               5                   10                  15      
Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Gln Gln Met Ile Lys 
            20                  25                  30          
Glu Leu Arg Gln Gly Ile Asp Glu Met Val Tyr Asp Ser Asp Ile Lys 
        35                  40                  45              
Val Ile Ile Ile Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Arg Phe Lys Glu Leu Asn Gly Tyr Thr Ala Trp Gln Phe Ala 
65                  70                  75                  80  
Lys Ser Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Ile Ser Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Val Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Met Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Val Arg Leu Ile Gly Lys Gly Lys Ala Leu Glu Leu Met Leu 
145                 150                 155                 160 
Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Lys Ile Gly Leu Val 
                165                 170                 175     
Asn Lys Val Val Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg Asn Phe 
            180                 185                 190         
Ala Leu Lys Leu Ala Glu Lys Pro Pro Ile Ser Ile Ala Leu Ile Lys 
        195                 200                 205             
Leu Leu Val Asn Gln Gly Ile Asp Leu Pro Ile Leu Ala Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Val Phe Ser Thr Glu Asp Glu Lys 
225                 230                 235                 240 
Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Gln Phe Lys Gly 
                245                 250                 255     
Lys 
    

<210> 37
<211> 258
<212> PRT
<213> Brevibacillus laterosporus LMG 15441

<220> 
<223> 3-hydroxypropionyl-coenzyme A dehydratase

<400> 37
Met Ser Tyr Pro Asn Leu Thr Leu Val Thr Glu Gly Thr Ile Ala Ile 
1               5                   10                  15      
Val Thr Ile Asn His Pro Pro Ala Asn Ala Leu Asn Gln Ala Thr Leu 
            20                  25                  30          
Thr Ser Leu Ala Gln Ala Leu Asp Asp Leu Glu Gln Asn Asp Gln Ile 
        35                  40                  45              
Arg Ala Ile Val Ile Thr Gly Glu Gly Arg Phe Phe Ile Ala Gly Ala 
    50                  55                  60                  
Asp Ile Lys Glu Phe Thr Ala Leu Ala Glu Gln Ser Pro Gln Gln Val 
65                  70                  75                  80  
Ala Glu Arg Gly Gln Gln Leu Phe Leu Arg Met Glu Thr Phe Ser Lys 
                85                  90                  95      
Pro Ile Ile Ala Ala Ile Asn Gly Ala Cys Leu Gly Gly Gly Leu Glu 
            100                 105                 110         
Leu Ala Met Ala Cys His Ile Arg Tyr Val Ala Lys Glu Ala Lys Leu 
        115                 120                 125             
Gly Leu Pro Glu Leu Asn Leu Gly Leu Ile Pro Gly Tyr Gly Gly Thr 
    130                 135                 140                 
Gln Arg Leu Pro Arg Leu Ile Gly Arg Gly Lys Ala Thr Gln Leu Ile 
145                 150                 155                 160 
Leu Thr Ser Asp Met Ile Asp Gly Glu Glu Ala Leu Ala Ile Gly Leu 
                165                 170                 175     
Ala Glu Ala Val Tyr Pro Val Glu Gln Leu Leu Glu Glu Ser Lys Lys 
            180                 185                 190         
Leu Ala Arg Lys Ile Ser Glu Lys Gly Ala Ile Ser Val Lys Tyr Ala 
        195                 200                 205             
Leu Asp Ala Ile His Ser Gly Val Glu Leu Gly Leu Ser Ala Gly Met 
    210                 215                 220                 
Lys Arg Glu Ala Glu Leu Phe Gly Gln Val Phe Thr Thr Glu Asp Met 
225                 230                 235                 240 
Lys Glu Gly Val Thr Ala Phe Leu Glu Lys Arg Lys Pro Gln Phe Ser 
                245                 250                 255     
Asn Arg 
        

<210> 38
<211> 257
<212> PRT
<213> Sulfolobus acidocaldarius DSM 639

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 38
Met Glu Thr Ile Ile Val Lys Asn Glu Pro Pro Phe Leu Arg Ile Thr 
1               5                   10                  15      
Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Lys Lys Met Ile Glu 
            20                  25                  30          
Glu Ile Arg Thr Val Leu Glu Glu Thr Val Lys Asp Glu Lys Val Arg 
        35                  40                  45              
Val Ile Ile Phe Thr Gly Asn Gly Arg Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Gln Phe Lys Glu Leu Glu Gly Leu Thr Ala Trp Gln Phe Ala 
65                  70                  75                  80  
Met Lys Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Tyr Pro Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Leu Ala Cys Asp Ile Arg Ile Ala Ser Asp Glu Ala Gln Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Thr Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Leu Lys Leu Val Gly Lys Ser Arg Thr Leu Glu Met Ile Met 
145                 150                 155                 160 
Leu Gly Glu Arg Ile Ser Ala Lys Asp Ala Glu Arg Ile Gly Leu Val 
                165                 170                 175     
Asn Arg Val Val Pro Ser Asn Asp Leu Glu Lys Glu Thr Leu Asn Leu 
            180                 185                 190         
Ala Ser Lys Leu Ala Glu Arg Pro Pro Leu Ala Ile Gln Leu Ser Lys 
        195                 200                 205             
Leu Ile Val Asn Gln Gly Met Asn Ser Pro Ile Thr Val Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Ile Phe Thr Thr Lys Asp Ser Lys 
225                 230                 235                 240 
Glu Gly Val Asn Ala Phe Leu Glu Lys Arg Lys Pro Asn Phe Lys Gly 
                245                 250                 255     
Glu 
    

<210> 39
<211> 257
<212> PRT
<213> Acidianus hospitalis W1

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 39
Met Glu Thr Val Glu Thr Lys Ile Glu Asn Gly Ile Gly Trp Ile Ile 
1               5                   10                  15      
Leu Asn Arg Pro Asp Lys Leu Asn Ala Ile Asn Leu Lys Met Leu Glu 
            20                  25                  30          
Glu Leu Glu Glu Val Thr Lys Asn Phe Glu Glu Asn Asn Asp Val Lys 
        35                  40                  45              
Ile Ile Ile Phe Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp 
    50                  55                  60                  
Ile Ser Gln Phe Lys Glu Leu Asn Ser Ile Ser Ala Trp Asn Phe Ala 
65                  70                  75                  80  
Lys Lys Gly Arg Arg Val Met Asp Tyr Ile Glu Ser Val Ser Lys Pro 
                85                  90                  95      
Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 
            100                 105                 110         
Ala Leu Ala Cys Asp Phe Arg Ile Ala Ala Glu Glu Ala Ser Leu Gly 
        115                 120                 125             
Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 
    130                 135                 140                 
Arg Leu Val Arg Ala Ile Gly Lys Ala Lys Ala Met Glu Leu Met Met 
145                 150                 155                 160 
Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Arg Ile Gly Leu Val 
                165                 170                 175     
Asn Lys Val Val Ser Leu Ser Ser Leu Lys Glu Glu Thr Ile Lys Phe 
            180                 185                 190         
Ala Gly Lys Leu Met Glu Lys Ser Pro Ile Ala Leu Ala Ile Leu Lys 
        195                 200                 205             
His Ile Ile Leu Tyr Gly Asn Asp Ser Pro Leu Leu Asp Gly Leu Asn 
    210                 215                 220                 
Met Glu Ser Leu Gly Trp Gly Val Ala Phe Ser Thr Glu Asp Glu Lys 
225                 230                 235                 240 
Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Val Phe Lys Gly 
                245                 250                 255     
Lys 
    

<210> 40
<211> 261
<212> PRT
<213> Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 40
Met Glu Leu Asn Asn Val Ile Leu Glu Lys Glu Gly Lys Val Ala Val 
1               5                   10                  15      
Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Asp Thr 
            20                  25                  30          
Leu Lys Glu Met Asp Tyr Val Ile Gly Glu Ile Glu Asn Asp Ser Glu 
        35                  40                  45              
Val Leu Ala Val Ile Leu Thr Gly Ala Gly Glu Lys Ser Phe Val Ala 
    50                  55                  60                  
Gly Ala Asp Ile Ser Glu Met Lys Glu Met Asn Thr Ile Glu Gly Arg 
65                  70                  75                  80  
Lys Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Arg Leu Glu Leu Leu 
                85                  90                  95      
Glu Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly 
            100                 105                 110         
Cys Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Ser Asn Ala 
        115                 120                 125             
Arg Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly 
    130                 135                 140                 
Gly Thr Gln Arg Leu Ser Arg Leu Val Gly Met Gly Met Ala Lys Gln 
145                 150                 155                 160 
Leu Ile Phe Thr Ala Gln Asn Ile Lys Ala Asp Glu Ala Leu Arg Ile 
                165                 170                 175     
Gly Leu Val Asn Lys Val Val Glu Pro Ser Glu Leu Met Asn Thr Ala 
            180                 185                 190         
Lys Glu Ile Ala Asn Lys Ile Val Ser Asn Ala Pro Val Ala Val Lys 
        195                 200                 205             
Leu Ser Lys Gln Ala Ile Asn Arg Gly Met Gln Cys Asp Ile Asp Thr 
    210                 215                 220                 
Ala Leu Ala Phe Glu Ser Glu Ala Phe Gly Glu Cys Phe Ser Thr Glu 
225                 230                 235                 240 
Asp Gln Lys Asp Ala Met Thr Ala Phe Ile Glu Lys Arg Lys Ile Glu 
                245                 250                 255     
Gly Phe Lys Asn Arg 
            260     

<210> 41
<211> 258
<212> PRT
<213> Myxococcus xanthus (strain DK 1622)

<220> 
<223> 3-hydroxybutyryl-CoA dehydratase

<400> 41
Met Pro Glu Phe Lys Val Asp Ala Arg Gly Pro Ile Glu Ile Trp Thr 
1               5                   10                  15      
Ile Asp Gly Glu Ser Arg Arg Asn Ala Ile Ser Arg Ala Met Leu Lys 
            20                  25                  30          
Glu Leu Gly Glu Leu Val Thr Arg Val Ser Ser Ser Arg Asp Val Arg 
        35                  40                  45              
Ala Val Val Ile Thr Gly Ala Gly Asp Lys Ala Phe Cys Ala Gly Ala 
    50                  55                  60                  
Asp Leu Lys Glu Arg Ala Thr Met Ala Glu Asp Glu Val Arg Ala Phe 
65                  70                  75                  80  
Leu Asp Gly Leu Arg Arg Thr Phe Arg Ala Ile Glu Lys Ser Asp Cys 
                85                  90                  95      
Val Phe Ile Ala Ala Ile Asn Gly Ala Ala Leu Gly Gly Gly Thr Glu 
            100                 105                 110         
Leu Ala Leu Ala Cys Asp Leu Arg Val Ala Ala Pro Ala Ala Glu Leu 
        115                 120                 125             
Gly Leu Thr Glu Val Lys Leu Gly Ile Ile Pro Gly Gly Gly Gly Thr 
    130                 135                 140                 
Gln Arg Leu Ala Arg Leu Val Gly Pro Gly Arg Ala Lys Asp Leu Ile 
145                 150                 155                 160 
Leu Thr Ala Arg Arg Ile Asn Ala Ala Glu Ala Phe Ser Val Gly Leu 
                165                 170                 175     
Ala Asn Arg Leu Ala Pro Glu Gly His Leu Leu Ala Val Ala Tyr Gly 
            180                 185                 190         
Leu Ala Glu Ser Val Val Glu Asn Ala Pro Ile Ala Val Ala Thr Ala 
        195                 200                 205             
Lys His Ala Ile Asp Glu Gly Thr Gly Leu Glu Leu Asp Asp Ala Leu 
    210                 215                 220                 
Ala Leu Glu Leu Arg Lys Tyr Glu Glu Ile Leu Lys Thr Glu Asp Arg 
225                 230                 235                 240 
Leu Glu Gly Leu Arg Ala Phe Ala Glu Lys Arg Ala Pro Val Tyr Lys 
                245                 250                 255     
Gly Arg 
        

<210> 42
<211> 301
<212> PRT
<213> Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787

<220> 
<223> phosphate butyryltransferase

<400> 42
Met Ile Lys Ser Phe Asn Glu Ile Ile Met Lys Val Lys Ser Lys Glu 
1               5                   10                  15      
Met Lys Lys Val Ala Val Ala Val Ala Gln Asp Glu Pro Val Leu Glu 
            20                  25                  30          
Ala Val Arg Asp Ala Lys Lys Asn Gly Ile Ala Asp Ala Ile Leu Val 
        35                  40                  45              
Gly Asp His Asp Glu Ile Val Ser Ile Ala Leu Lys Ile Gly Met Asp 
    50                  55                  60                  
Val Asn Asp Phe Glu Ile Val Asn Glu Pro Asn Val Lys Lys Ala Ala 
65                  70                  75                  80  
Leu Lys Ala Val Glu Leu Val Ser Thr Gly Lys Ala Asp Met Val Met 
                85                  90                  95      
Lys Gly Leu Val Asn Thr Ala Thr Phe Leu Arg Ser Val Leu Asn Lys 
            100                 105                 110         
Glu Val Gly Leu Arg Thr Gly Lys Thr Met Ser His Val Ala Val Phe 
        115                 120                 125             
Glu Thr Glu Lys Phe Asp Arg Leu Leu Phe Leu Thr Asp Val Ala Phe 
    130                 135                 140                 
Asn Thr Tyr Pro Glu Leu Lys Glu Lys Ile Asp Ile Val Asn Asn Ser 
145                 150                 155                 160 
Val Lys Val Ala His Ala Ile Gly Ile Glu Asn Pro Lys Val Ala Pro 
                165                 170                 175     
Ile Cys Ala Val Glu Val Ile Asn Pro Lys Met Pro Ser Thr Leu Asp 
            180                 185                 190         
Ala Ala Met Leu Ser Lys Met Ser Asp Arg Gly Gln Ile Lys Gly Cys 
        195                 200                 205             
Val Val Asp Gly Pro Leu Ala Leu Asp Ile Ala Leu Ser Glu Glu Ala 
    210                 215                 220                 
Ala His His Lys Gly Val Thr Gly Glu Val Ala Gly Lys Ala Asp Ile 
225                 230                 235                 240 
Phe Leu Met Pro Asn Ile Glu Thr Gly Asn Val Met Tyr Lys Thr Leu 
                245                 250                 255     
Thr Tyr Thr Thr Asp Ser Lys Asn Gly Gly Ile Leu Val Gly Thr Ser 
            260                 265                 270         
Ala Pro Val Val Leu Thr Ser Arg Ala Asp Ser His Glu Thr Lys Met 
        275                 280                 285             
Asn Ser Ile Ala Leu Ala Ala Leu Val Ala Gly Asn Lys 
    290                 295                 300     

<210> 43
<211> 355
<212> PRT
<213> Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787

<220> 
<223> butyrate kinase 1

<400> 43
Met Tyr Arg Leu Leu Ile Ile Asn Pro Gly Ser Thr Ser Thr Lys Ile 
1               5                   10                  15      
Gly Ile Tyr Asp Asp Glu Lys Glu Ile Phe Glu Lys Thr Leu Arg His 
            20                  25                  30          
Ser Ala Glu Glu Ile Glu Lys Tyr Asn Thr Ile Phe Asp Gln Phe Gln 
        35                  40                  45              
Phe Arg Lys Asn Val Ile Leu Asp Ala Leu Lys Glu Ala Asn Ile Glu 
    50                  55                  60                  
Val Ser Ser Leu Asn Ala Val Val Gly Arg Gly Gly Leu Leu Lys Pro 
65                  70                  75                  80  
Ile Val Ser Gly Thr Tyr Ala Val Asn Gln Lys Met Leu Glu Asp Leu 
                85                  90                  95      
Lys Val Gly Val Gln Gly Gln His Ala Ser Asn Leu Gly Gly Ile Ile 
            100                 105                 110         
Ala Asn Glu Ile Ala Lys Glu Ile Asn Val Pro Ala Tyr Ile Val Asp 
        115                 120                 125             
Pro Val Val Val Asp Glu Leu Asp Glu Val Ser Arg Ile Ser Gly Met 
    130                 135                 140                 
Ala Asp Ile Pro Arg Lys Ser Ile Phe His Ala Leu Asn Gln Lys Ala 
145                 150                 155                 160 
Val Ala Arg Arg Tyr Ala Lys Glu Val Gly Lys Lys Tyr Glu Asp Leu 
                165                 170                 175     
Asn Leu Ile Val Val His Met Gly Gly Gly Thr Ser Val Gly Thr His 
            180                 185                 190         
Lys Asp Gly Arg Val Ile Glu Val Asn Asn Thr Leu Asp Gly Glu Gly 
        195                 200                 205             
Pro Phe Ser Pro Glu Arg Ser Gly Gly Val Pro Ile Gly Asp Leu Val 
    210                 215                 220                 
Arg Leu Cys Phe Ser Asn Lys Tyr Thr Tyr Glu Glu Val Met Lys Lys 
225                 230                 235                 240 
Ile Asn Gly Lys Gly Gly Val Val Ser Tyr Leu Asn Thr Ile Asp Phe 
                245                 250                 255     
Lys Ala Val Val Asp Lys Ala Leu Glu Gly Asp Lys Lys Cys Ala Leu 
            260                 265                 270         
Ile Tyr Glu Ala Phe Thr Phe Gln Val Ala Lys Glu Ile Gly Lys Cys 
        275                 280                 285             
Ser Thr Val Leu Lys Gly Asn Val Asp Ala Ile Ile Leu Thr Gly Gly 
    290                 295                 300                 
Ile Ala Tyr Asn Glu His Val Cys Asn Ala Ile Glu Asp Arg Val Lys 
305                 310                 315                 320 
Phe Ile Ala Pro Val Val Arg Tyr Gly Gly Glu Asp Glu Leu Leu Ala 
                325                 330                 335     
Leu Ala Glu Gly Gly Leu Arg Val Leu Arg Gly Glu Glu Lys Ala Lys 
            340                 345                 350         
Glu Tyr Lys 
        355 

