                         SEQUENCE LISTING

<110>  Herzog, Christian
 
<120>  Biomarker, sowie Verfahren zur Bestimmung des Risikos einer 
       Arthrofibrose

<130>  X3423 PCT BS

<150>  EP 14198713.1
<151>  2014-12-18

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vorwrtsprimer

<400>  1
ctccagcaga agccacctaa ag                                                22


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rckwrtsprimer

<400>  2
tccatgagag gctggtagca ct                                                22


<210>  3
<211>  10
<212>  PRT
<213>  Ras inhibitory peptide

<400>  3

Val Pro Pro Pro Val Pro Pro Arg Arg Arg 
1               5                   10  


<210>  4
<211>  804
<212>  PRT
<213>  Homo sapiens RasGAP-activating-like protein 1

<400>  4

Met Ala Lys Ser Ser Ser Leu Asn Val Arg Val Val Glu Gly Arg Ala 
1               5                   10                  15      


Leu Pro Ala Lys Asp Val Ser Gly Ser Ser Asp Pro Tyr Cys Leu Val 
            20                  25                  30          


Lys Val Asp Asp Glu Val Val Ala Arg Thr Ala Thr Val Trp Arg Ser 
        35                  40                  45              


Leu Gly Pro Phe Trp Gly Glu Glu Tyr Thr Val His Leu Pro Leu Asp 
    50                  55                  60                  


Phe His Gln Leu Ala Phe Tyr Val Leu Asp Glu Asp Thr Val Gly His 
65                  70                  75                  80  


Asp Asp Ile Ile Gly Lys Ile Ser Leu Ser Arg Glu Ala Ile Thr Ala 
                85                  90                  95      


Asp Pro Arg Gly Ile Asp Ser Trp Ile Asn Leu Ser Arg Val Asp Pro 
            100                 105                 110         


Asp Ala Glu Val Gln Gly Glu Ile Cys Leu Ser Val Gln Met Leu Glu 
        115                 120                 125             


Asp Gly Gln Gly Arg Cys Leu Arg Cys His Val Leu Gln Ala Arg Asp 
    130                 135                 140                 


Leu Ala Pro Arg Asp Ile Ser Gly Thr Ser Asp Pro Phe Ala Arg Val 
145                 150                 155                 160 


Phe Trp Gly Ser Gln Ser Leu Glu Thr Ser Thr Ile Lys Lys Thr Arg 
                165                 170                 175     


Phe Pro His Trp Asp Glu Val Leu Glu Leu Arg Glu Met Pro Gly Ala 
            180                 185                 190         


Pro Ser Pro Leu Arg Val Glu Leu Trp Asp Trp Asp Met Val Gly Lys 
        195                 200                 205             


Asn Asp Phe Leu Gly Met Val Glu Phe Ser Pro Lys Thr Leu Gln Gln 
    210                 215                 220                 


Lys Pro Pro Lys Gly Trp Phe Arg Leu Leu Pro Phe Pro Arg Ala Glu 
225                 230                 235                 240 


Glu Asp Ser Gly Gly Asn Leu Gly Ala Leu Arg Val Lys Val Arg Leu 
                245                 250                 255     


Ile Glu Asp Arg Val Leu Pro Ser Gln Cys Tyr Gln Pro Leu Met Glu 
            260                 265                 270         


Leu Leu Met Glu Ser Val Gln Gly Pro Ala Glu Glu Asp Thr Ala Ser 
        275                 280                 285             


Pro Leu Ala Leu Leu Glu Glu Leu Thr Leu Gly Asp Cys Arg Gln Asp 
    290                 295                 300                 


Leu Ala Thr Lys Leu Val Lys Leu Phe Leu Gly Arg Gly Leu Ala Gly 
305                 310                 315                 320 


Arg Phe Leu Asp Tyr Leu Thr Arg Arg Glu Val Ala Arg Thr Met Asp 
                325                 330                 335     


Pro Asn Thr Leu Phe Arg Ser Asn Ser Leu Ala Ser Lys Ser Met Glu 
            340                 345                 350         


Gln Phe Met Lys Leu Val Gly Met Pro Tyr Leu His Glu Val Leu Lys 
        355                 360                 365             


Pro Val Ile Ser Arg Val Phe Glu Glu Lys Lys Tyr Met Glu Leu Asp 
    370                 375                 380                 


Pro Cys Lys Met Asp Leu Gly Arg Thr Arg Arg Ile Ser Phe Lys Gly 
385                 390                 395                 400 


Ala Leu Ser Glu Glu Gln Met Arg Glu Thr Ser Leu Gly Leu Leu Thr 
                405                 410                 415     


Gly Tyr Leu Gly Pro Ile Val Asp Ala Ile Val Gly Ser Val Gly Arg 
            420                 425                 430         


Cys Pro Pro Ala Met Arg Leu Ala Phe Lys Gln Leu His Arg Arg Val 
        435                 440                 445             


Glu Glu Arg Phe Pro Gln Ala Glu His Gln Asp Val Lys Tyr Leu Ala 
    450                 455                 460                 


Ile Ser Gly Phe Leu Phe Leu Arg Phe Phe Ala Pro Ala Ile Leu Thr 
465                 470                 475                 480 


Pro Lys Leu Phe Asp Leu Arg Asp Gln His Ala Asp Pro Gln Thr Ser 
                485                 490                 495     


Arg Ser Leu Leu Leu Leu Ala Lys Ala Val Gln Ser Ile Gly Asn Leu 
            500                 505                 510         


Gly Gln Gln Leu Gly Gln Gly Lys Glu Leu Trp Met Ala Pro Leu His 
        515                 520                 525             


Pro Phe Leu Leu Gln Cys Val Ser Arg Val Arg Asp Phe Leu Asp Arg 
    530                 535                 540                 


Leu Val Asp Val Asp Gly Asp Glu Ala Gly Val Pro Ala Arg Ala Leu 
545                 550                 555                 560 


Phe Pro Pro Ser Ala Ile Val Arg Glu Gly Tyr Leu Leu Lys Arg Lys 
                565                 570                 575     


Glu Glu Pro Ala Gly Leu Ala Thr Arg Phe Ala Phe Lys Lys Arg Tyr 
            580                 585                 590         


Val Trp Leu Ser Gly Glu Thr Leu Ser Phe Ser Lys Ser Pro Glu Trp 
        595                 600                 605             


Gln Met Cys His Ser Ile Pro Val Ser His Ile Arg Ala Val Glu Arg 
    610                 615                 620                 


Val Asp Glu Gly Ala Phe Gln Leu Pro His Val Met Gln Val Val Thr 
625                 630                 635                 640 


Gln Asp Gly Thr Gly Ala Leu His Thr Thr Tyr Leu Gln Cys Lys Asn 
                645                 650                 655     


Val Asn Glu Leu Asn Gln Trp Leu Ser Ala Leu Arg Lys Ala Ser Ala 
            660                 665                 670         


Pro Asn Pro Asn Lys Leu Ala Ala Cys His Pro Gly Ala Phe Arg Ser 
        675                 680                 685             


Ala Arg Trp Thr Cys Cys Leu Gln Ala Glu Arg Ser Ala Ala Gly Cys 
    690                 695                 700                 


Ser Arg Thr His Ser Ala Val Thr Leu Gly Asp Trp Ser Asp Pro Leu 
705                 710                 715                 720 


Asp Pro Asp Ala Glu Ala Gln Thr Val Tyr Arg Gln Leu Leu Leu Gly 
                725                 730                 735     


Arg Asp Gln Leu Arg Leu Lys Leu Leu Glu Asp Ser Asn Met Asp Thr 
            740                 745                 750         


Thr Leu Glu Ala Asp Thr Gly Ala Cys Pro Glu Val Leu Ala Arg Gln 
        755                 760                 765             


Arg Ala Ala Thr Ala Arg Leu Leu Glu Val Leu Ala Asp Leu Asp Arg 
    770                 775                 780                 


Ala His Glu Glu Phe Gln Gln Gln Glu Arg Gly Lys Ala Ala Leu Gly 
785                 790                 795                 800 


Pro Leu Gly Pro 
                


<210>  5
<211>  2331
<212>  DNA
<213>  Homo sapiens

<400>  5
atggccaaga gcagctccct gaatgttcgc gtggtggagg gccgcgcgct gcctgccaag       60

gacgtgtctg ggagcagcga cccctactgc ctagtgaaag tggacgacga ggtggtggcc      120

aggacagcta ctgtctggag gagcctgggc cccttctggg gggaggagta cacggtgcac      180

ctgcctctgg atttccacca gctggccttc tacgtgctgg atgaggacac tgtcgggcac      240

gacgacatca tcggcaagat ctcgctgagc agggaggcga ttacagccga cccccgaggg      300

attgacagct ggattaactt gagccgagtg gacccagatg cagaagtgca gggtgagatc      360

tgcctgtcag tgcagatgct ggaggatggg cagggccgct gccttcgctg ccatgtgctt      420

caggccaggg acctggctcc cagagacatc tctggcacat ctgacccatt tgcacgtgtg      480

ttttggggca gccagagctt ggagacctca accatcaaga agactcgctt cccgcactgg      540

gatgaagtgc tggagctgcg ggagatgcca ggtgccccgt ccccactgcg ggtggagctc      600

tgggactggg acatggtggg caagaatgac ttcttgggca tggtggagtt ctctccaaag      660

accctccagc agaagccacc taaaggctgg ttccgcctcc tgccctttcc cagagccgag      720

gaggattctg gggggaacct gggtgccctg cgagtgaagg tacgcctgat tgaggaccgc      780

gtcctgccct cccagtgcta ccagcctctc atggagctgc tcatggagtc tgtgcagggg      840

ccagcagagg aggacactgc tagccccttg gctttgctgg aagagctgac cttgggggac      900

tgccgccagg accttgccac caagctggtg aaactctttc ttggccgggg actggctggg      960

cactttctgg actatctcac ccggcgtgag gtggctcgga ccatggaccc caacaccctc     1020

ttccgttcta actccctggc atccaagtcg atggaacagt ttatgaagct cgtgggcatg     1080

ccctacctgc acgaggtcct gaagcctgtg attagccgtg tctttgagga gaagaagtac     1140

atggagctgg atccctgcaa gatggacctg ggccgcaccc ggaggatctc cttcaaaggc     1200

gcactctcgg aggagcagat gcgggagacc agcctggggc tgctgacggg ctacctgggg     1260

cccatcgtgg acgccatcgt gggctccgtg gggcgctgcc cgcccgccat gcgcctcgcc     1320

ttcaagcagc tgcaccggcg agtggaggag cgcttccccc aggccgagca ccaggatgtg     1380

aagtacctgg ccatcagtgg atttctcttc ttgcgattct tcgcacctgc catccttacc     1440

ccaaagctgt ttgaccttcg ggaccaacac gcggaccccc agactagccg ctcactgctg     1500

ttgcttgcca aggctgtgca gagcattgga aacctgggcc agcagctggg ccaaggcaag     1560

gaactgtgga tggcccccct gcaccccttc ctgctgcagt gtgtctcacg tgtgagagac     1620

ttcctggacc ggctggtgga tgtggatggg gatgaagctg gtgtcccagc cagggccctg     1680

ttcccgccct cggccattgt tcgagaaggc tatctgctga agcgcaagga ggagcctgcc     1740

ggcctggcca cgcgctttgc cttcaagaag cgctacgtct ggctcagcgg ggagaccctc     1800

tccttctcca agagtcctga gtggcaggtg gtgacgcagg acggcacggg ggcgctgcac     1860

accacctacc tccagtgcaa gaatgtgaat gagctcaacc agtggctctc ggccttgcgc     1920

aaggccagcg cccccaaccc gaacaagctg gccgcctgcc accccggtgc cttccgcagc     1980

gcgcgctgga cctgctgcct ccaggctgag cgctcagccg ccggctgcag ccgtacacac     2040

tcagctgtca ccctggggga ctggagtgac ccactggatc ctgatgctga ggcccagaca     2100

gtgtatcggc agctgctcct ggggcgggac cagctcaggc tgaaattact ggaggattct     2160

aacatggata caactctgga ggcagacaca ggggcctgtc ctgaggtcct ggcccggcaa     2220

agagcagcaa ctgcccgcct gctggaggtg ctcgcagacc tggatcgtgc ccacgaggag     2280

ttccagcagc aggagcgagg gaaggcggcc ctgggccccc ttggccccta a              2331


<210>  6
<211>  776
<212>  PRT
<213>  Homo sapiens Isoform 2 of RasGAP-activating-like protein 1

<400>  6

Met Ala Lys Ser Ser Ser Leu Asn Val Arg Val Val Glu Gly Arg Ala 
1               5                   10                  15      


Leu Pro Ala Lys Asp Val Ser Gly Ser Ser Asp Pro Tyr Cys Leu Val 
            20                  25                  30          


Lys Val Asp Asp Glu Val Val Ala Arg Thr Ala Thr Val Trp Arg Ser 
        35                  40                  45              


Leu Gly Pro Phe Trp Gly Glu Glu Tyr Thr Val His Leu Pro Leu Asp 
    50                  55                  60                  


Phe His Gln Leu Ala Phe Tyr Val Leu Asp Glu Asp Thr Val Gly His 
65                  70                  75                  80  


Asp Asp Ile Ile Gly Lys Ile Ser Leu Ser Arg Glu Ala Ile Thr Ala 
                85                  90                  95      


Asp Pro Arg Gly Ile Asp Ser Trp Ile Asn Leu Ser Arg Val Asp Pro 
            100                 105                 110         


Asp Ala Glu Val Gln Gly Glu Ile Cys Leu Ser Val Gln Met Leu Glu 
        115                 120                 125             


Asp Gly Gln Gly Arg Cys Leu Arg Cys His Val Leu Gln Ala Arg Asp 
    130                 135                 140                 


Leu Ala Pro Arg Asp Ile Ser Gly Thr Ser Asp Pro Phe Ala Arg Val 
145                 150                 155                 160 


Phe Trp Gly Ser Gln Ser Leu Glu Thr Ser Thr Ile Lys Lys Thr Arg 
                165                 170                 175     


Phe Pro His Trp Asp Glu Val Leu Glu Leu Arg Glu Met Pro Gly Ala 
            180                 185                 190         


Pro Ser Pro Leu Arg Val Glu Leu Trp Asp Trp Asp Met Val Gly Lys 
        195                 200                 205             


Asn Asp Phe Leu Gly Met Val Glu Phe Ser Pro Lys Thr Leu Gln Gln 
    210                 215                 220                 


Lys Pro Pro Lys Gly Trp Phe Arg Leu Leu Pro Phe Pro Arg Ala Glu 
225                 230                 235                 240 


Glu Asp Ser Gly Gly Asn Leu Gly Ala Leu Arg Val Lys Val Arg Leu 
                245                 250                 255     


Ile Glu Asp Arg Val Leu Pro Ser Gln Cys Tyr Gln Pro Leu Met Glu 
            260                 265                 270         


Leu Leu Met Glu Ser Val Gln Gly Pro Ala Glu Glu Asp Thr Ala Ser 
        275                 280                 285             


Pro Leu Ala Leu Leu Glu Glu Leu Thr Leu Gly Asp Cys Arg Gln Asp 
    290                 295                 300                 


Leu Ala Thr Lys Leu Val Lys Leu Phe Leu Gly Arg Gly Leu Ala Gly 
305                 310                 315                 320 


Arg Phe Leu Asp Tyr Leu Thr Arg Arg Glu Val Ala Arg Thr Met Asp 
                325                 330                 335     


Pro Asn Thr Leu Phe Arg Ser Asn Ser Leu Ala Ser Lys Ser Met Glu 
            340                 345                 350         


Gln Phe Met Lys Leu Val Gly Met Pro Tyr Leu His Glu Val Leu Lys 
        355                 360                 365             


Pro Val Ile Ser Arg Val Phe Glu Glu Lys Lys Tyr Met Glu Leu Asp 
    370                 375                 380                 


Pro Cys Lys Met Asp Leu Gly Arg Thr Arg Arg Ile Ser Phe Lys Gly 
385                 390                 395                 400 


Ala Leu Ser Glu Glu Gln Met Arg Glu Thr Ser Leu Gly Leu Leu Thr 
                405                 410                 415     


Gly Tyr Leu Gly Pro Ile Val Asp Ala Ile Val Gly Ser Val Gly Arg 
            420                 425                 430         


Cys Pro Pro Ala Met Arg Leu Ala Phe Lys Gln Leu His Arg Arg Val 
        435                 440                 445             


Glu Glu Arg Phe Pro Gln Ala Glu His Gln Asp Val Lys Tyr Leu Ala 
    450                 455                 460                 


Ile Ser Gly Phe Leu Phe Leu Arg Phe Phe Ala Pro Ala Ile Leu Thr 
465                 470                 475                 480 


Pro Lys Leu Phe Asp Leu Arg Asp Gln His Ala Asp Pro Gln Thr Ser 
                485                 490                 495     


Arg Ser Leu Leu Leu Leu Ala Lys Ala Val Gln Ser Ile Gly Asn Leu 
            500                 505                 510         


Gly Gln Gln Leu Gly Gln Gly Lys Glu Leu Trp Met Ala Pro Leu His 
        515                 520                 525             


Pro Phe Leu Leu Gln Cys Val Ser Arg Val Arg Asp Phe Leu Asp Arg 
    530                 535                 540                 


Leu Val Asp Val Asp Gly Asp Glu Ala Gly Val Pro Ala Arg Ala Leu 
545                 550                 555                 560 


Phe Pro Pro Ser Ala Ile Val Arg Glu Gly Tyr Leu Leu Lys Arg Lys 
                565                 570                 575     


Glu Glu Pro Ala Gly Leu Ala Thr Arg Phe Ala Phe Lys Lys Arg Tyr 
            580                 585                 590         


Val Trp Leu Ser Gly Glu Thr Leu Ser Phe Ser Lys Ser Pro Glu Trp 
        595                 600                 605             


Gln Val Val Thr Gln Asp Gly Thr Gly Ala Leu His Thr Thr Tyr Leu 
    610                 615                 620                 


Gln Cys Lys Asn Val Asn Glu Leu Asn Gln Trp Leu Ser Ala Leu Arg 
625                 630                 635                 640 


Lys Ala Ser Ala Pro Asn Pro Asn Lys Leu Ala Ala Cys His Pro Gly 
                645                 650                 655     


Ala Phe Arg Ser Ala Arg Trp Thr Cys Cys Leu Gln Ala Glu Arg Ser 
            660                 665                 670         


Ala Ala Gly Cys Ser Arg Thr His Ser Ala Val Thr Leu Gly Asp Trp 
        675                 680                 685             


Ser Asp Pro Leu Asp Pro Asp Ala Glu Ala Gln Thr Val Tyr Arg Gln 
    690                 695                 700                 


Leu Leu Leu Gly Arg Asp Gln Leu Arg Leu Lys Leu Leu Glu Asp Ser 
705                 710                 715                 720 


Asn Met Asp Thr Thr Leu Glu Ala Asp Thr Gly Ala Cys Pro Glu Val 
                725                 730                 735     


Leu Ala Arg Gln Arg Ala Ala Thr Ala Arg Leu Leu Glu Val Leu Ala 
            740                 745                 750         


Asp Leu Asp Arg Ala His Glu Glu Phe Gln Gln Gln Glu Arg Gly Lys 
        755                 760                 765             


Ala Ala Leu Gly Pro Leu Gly Pro 
    770                 775     


<210>  7
<211>  2328
<212>  DNA
<213>  Homo sapiens

<400>  7
atggcgaaaa gcagcagcct gaacgtgcgc gtggtggaag gccgcgcgct gccggcgaaa       60

gatgtgagcg gcagcagcga tccgtattgc ctggtgaaag tggatgatga agtggtggcg      120

cgcaccgcga ccgtgtggcg cagcctgggc ccgttttggg gcgaagaata taccgtgcat      180

ctgccgctgg attttcatca gctggcgttt tatgtgctgg atgaagatac cgtgggccat      240

gatgatatta ttggcaaaat tagcctgagc cgcgaagcga ttaccgcgga tccgcgcggc      300

attgatagct ggattaacct gagccgcgtg gatccggatg cggaagtgca gggcgaaatt      360

tgcctgagcg tgcagatgct ggaagatggc cagggccgct gcctgcgctg ccatgtgctg      420

caggcgcgcg atctggcgcc gcgcgatatt agcggcacca gcgatccgtt tgcgcgcgtg      480

ttttggggca gccagagcct ggaaaccagc accattaaaa aaacccgctt tccgcattgg      540

gatgaagtgc tggaactgcg cgaaatgccg ggcgcgccga gcccgctgcg cgtggaactg      600

tgggattggg atatggtggg caaaaacgat tttctgggca tggtggaatt tagcccgaaa      660

accctgcagc agaaaccgcc gaaaggctgg tttcgcctgc tgccgtttcc gcgcgcggaa      720

gaagatagcg gcggcaacct gggcgcgctg cgcgtgaaag tgcgcctgat tgaagatcgc      780

gtgctgccga gccagtgcta tcagccgctg atggaactgc tgatggaaag cgtgcagggc      840

ccggcggaag aagataccgc gagcccgctg gcgctgctgg aagaactgac cctgggcgat      900

tgccgccagg atctggcgac caaactggtg aaactgtttc tgggccgcgg cctggcgggc      960

cgctttctgg attatctgac ccgccgcgaa gtggcgcgca ccatggatcc gaacaccctg     1020

tttcgcagca acagcctggc gagcaaaagc atggaacagt ttatgaaact ggtgggcatg     1080

ccgtatctgc atgaagtgct gaaaccggtg attagccgcg tgtttgaaga aaaaaaatat     1140

atggaactgg atccgtgcaa aatggatctg ggccgcaccc gccgcattag ctttaaaggc     1200

gcgctgagcg aagaacagat gcgcgaaacc agcctgggcc tgctgaccgg ctatctgggc     1260

ccgattgtgg atgcgattgt gggcagcgtg ggccgctgcc cgccggcgat gcgcctggcg     1320

tttaaacagc tgcatcgccg cgtggaagaa cgctttccgc aggcggaaca tcaggatgtg     1380

aaatatctgg cgattagcgg ctttctgttt ctgcgctttt ttgcgccggc gattctgacc     1440

ccgaaactgt ttgatctgcg cgatcagcat gcggatccgc agaccagccg cagcctgctg     1500

ctgctggcga aagcggtgca gagcattggc aacctgggcc agcagctggg ccagggcaaa     1560

gaactgtgga tggcgccgct gcatccgttt ctgctgcagt gcgtgagccg cgtgcgcgat     1620

tttctggatc gcctggtgga tgtggatggc gatgaagcgg gcgtgccggc gcgcgcgctg     1680

tttccgccga gcgcgattgt gcgcgaaggc tatctgctga aacgcaaaga agaaccggcg     1740

ggcctggcga cccgctttgc gtttaaaaaa cgctatgtgt ggctgagcgg cgaaaccctg     1800

agctttagca aaagcccgga atggcaggtg gtgacccagg atggcaccgg cgcgctgcat     1860

accacctatc tgcagtgcaa aaacgtgaac gaactgaacc agtggctgag cgcgctgcgc     1920

aaagcgagcg cgccgaaccc gaacaaactg gcggcgtgcc atccgggcgc gtttcgcagc     1980

gcgcgctgga cctgctgcct gcaggcggaa cgcagcgcgg cgggctgcag ccgcacccat     2040

agcgcggtga ccctgggcga ttggagcgat ccgctggatc cggatgcgga agcgcagacc     2100

gtgtatcgcc agctgctgct gggccgcgat cagctgcgcc tgaaactgct ggaagatagc     2160

aacatggata ccaccctgga agcggatacc ggcgcgtgcc cggaagtgct ggcgcgccag     2220

cgcgcggcga ccgcgcgcct gctggaagtg ctggcggatc tggatcgcgc gcatgaagaa     2280

tttcagcagc aggaacgcgg caaagcggcg ctgggcccgc tgggcccg                  2328


<210>  8
<211>  806
<212>  PRT
<213>  Homo sapiens Isoform 3 of RasGAP-activating-like protein 1

<400>  8

Met Ala Lys Ser Ser Ser Leu Asn Val Arg Val Val Glu Gly Arg Ala 
1               5                   10                  15      


Leu Pro Ala Lys Asp Val Ser Gly Ser Ser Asp Pro Tyr Cys Leu Val 
            20                  25                  30          


Lys Val Asp Asp Glu Val Val Ala Arg Thr Ala Thr Val Trp Arg Ser 
        35                  40                  45              


Leu Gly Pro Phe Trp Gly Glu Glu Tyr Thr Val His Leu Pro Leu Asp 
    50                  55                  60                  


Phe His Gln Leu Ala Phe Tyr Val Leu Asp Glu Asp Thr Val Gly His 
65                  70                  75                  80  


Asp Asp Ile Ile Gly Lys Ile Ser Leu Ser Arg Glu Ala Ile Thr Ala 
                85                  90                  95      


Asp Pro Arg Gly Ile Asp Ser Trp Ile Asn Leu Ser Arg Val Asp Pro 
            100                 105                 110         


Asp Ala Glu Val Gln Gly Glu Ile Cys Leu Ser Val Gln Met Leu Glu 
        115                 120                 125             


Asp Gly Gln Gly Arg Cys Leu Arg Cys His Val Leu Gln Ala Arg Asp 
    130                 135                 140                 


Leu Ala Pro Arg Asp Ile Ser Gly Thr Ser Asp Pro Phe Ala Arg Val 
145                 150                 155                 160 


Phe Trp Gly Ser Gln Ser Leu Glu Thr Ser Thr Ile Lys Lys Thr Arg 
                165                 170                 175     


Phe Pro His Trp Asp Glu Val Leu Glu Leu Arg Glu Met Pro Gly Ala 
            180                 185                 190         


Pro Ser Pro Leu Arg Val Glu Leu Trp Asp Trp Asp Met Val Gly Lys 
        195                 200                 205             


Asn Asp Phe Leu Gly Met Val Glu Phe Ser Pro Lys Thr Leu Gln Gln 
    210                 215                 220                 


Lys Pro Pro Lys Gly Trp Phe Arg Leu Leu Pro Phe Pro Arg Ala Glu 
225                 230                 235                 240 


Glu Asp Ser Gly Gly Asn Leu Gly Ala Leu Arg Val Lys Val Arg Leu 
                245                 250                 255     


Ile Glu Asp Arg Val Leu Pro Ser Gln Cys Tyr Gln Pro Leu Met Glu 
            260                 265                 270         


Leu Leu Met Glu Ser Val Gln Gly Pro Ala Glu Glu Asp Thr Ala Ser 
        275                 280                 285             


Pro Leu Ala Leu Leu Glu Glu Leu Thr Leu Gly Asp Cys Arg Gln Asp 
    290                 295                 300                 


Leu Ala Thr Lys Leu Val Lys Leu Phe Leu Gly Arg Gly Leu Ala Gly 
305                 310                 315                 320 


Arg Phe Leu Asp Tyr Leu Thr Arg Arg Glu Val Ala Arg Thr Met Asp 
                325                 330                 335     


Pro Asn Thr Leu Phe Arg Ser Asn Ser Leu Ala Ser Lys Ser Met Glu 
            340                 345                 350         


Gln Phe Met Lys Leu Val Gly Met Pro Tyr Leu His Glu Val Leu Lys 
        355                 360                 365             


Pro Val Ile Ser Arg Val Phe Glu Glu Lys Lys Tyr Met Glu Leu Asp 
    370                 375                 380                 


Pro Cys Lys Met Asp Leu Gly Arg Thr Arg Arg Ile Ser Phe Lys Gly 
385                 390                 395                 400 


Ala Leu Ser Glu Glu Gln Met Arg Glu Thr Ser Leu Gly Leu Leu Thr 
                405                 410                 415     


Gly Tyr Leu Gly Pro Ile Val Asp Ala Ile Val Gly Ser Val Gly Arg 
            420                 425                 430         


Cys Pro Pro Ala Met Arg Leu Ala Phe Lys Gln Leu His Arg Arg Val 
        435                 440                 445             


Glu Glu Arg Phe Pro Gln Ala Glu His Gln Gln Asp Val Lys Tyr Leu 
    450                 455                 460                 


Ala Ile Ser Gly Phe Leu Phe Leu Arg Phe Phe Ala Pro Ala Ile Leu 
465                 470                 475                 480 


Thr Pro Lys Leu Phe Asp Leu Arg Asp Gln His Ala Asp Pro Gln Thr 
                485                 490                 495     


Ser Arg Ser Leu Leu Leu Leu Ala Lys Ala Val Gln Ser Ile Gly Asn 
            500                 505                 510         


Leu Gly Gln Gln Leu Gly Gln Gly Lys Glu Leu Trp Met Ala Pro Leu 
        515                 520                 525             


His Pro Phe Leu Leu Gln Cys Val Ser Arg Val Arg Asp Phe Leu Asp 
    530                 535                 540                 


Arg Leu Val Asp Val Asp Gly Asp Glu Glu Ala Gly Val Pro Ala Arg 
545                 550                 555                 560 


Ala Leu Phe Pro Pro Ser Ala Ile Val Arg Glu Gly Tyr Leu Leu Lys 
                565                 570                 575     


Arg Lys Glu Glu Pro Ala Gly Leu Ala Thr Arg Phe Ala Phe Lys Lys 
            580                 585                 590         


Arg Tyr Val Trp Leu Ser Gly Glu Thr Leu Ser Phe Ser Lys Ser Pro 
        595                 600                 605             


Glu Trp Gln Met Cys His Ser Ile Pro Val Ser His Ile Arg Ala Val 
    610                 615                 620                 


Glu Arg Val Asp Glu Gly Ala Phe Gln Leu Pro His Val Met Gln Val 
625                 630                 635                 640 


Val Thr Gln Asp Gly Thr Gly Ala Leu His Thr Thr Tyr Leu Gln Cys 
                645                 650                 655     


Lys Asn Val Asn Glu Leu Asn Gln Trp Leu Ser Ala Leu Arg Lys Ala 
            660                 665                 670         


Ser Ala Pro Asn Pro Asn Lys Leu Ala Ala Cys His Pro Gly Ala Phe 
        675                 680                 685             


Arg Ser Ala Arg Trp Thr Cys Cys Leu Gln Ala Glu Arg Ser Ala Ala 
    690                 695                 700                 


Gly Cys Ser Arg Thr His Ser Ala Val Thr Leu Gly Asp Trp Ser Asp 
705                 710                 715                 720 


Pro Leu Asp Pro Asp Ala Glu Ala Gln Thr Val Tyr Arg Gln Leu Leu 
                725                 730                 735     


Leu Gly Arg Asp Gln Leu Arg Leu Lys Leu Leu Glu Asp Ser Asn Met 
            740                 745                 750         


Asp Thr Thr Leu Glu Ala Asp Thr Gly Ala Cys Pro Glu Val Leu Ala 
        755                 760                 765             


Arg Gln Arg Ala Ala Thr Ala Arg Leu Leu Glu Val Leu Ala Asp Leu 
    770                 775                 780                 


Asp Arg Ala His Glu Glu Phe Gln Gln Gln Glu Arg Gly Lys Ala Ala 
785                 790                 795                 800 


Leu Gly Pro Leu Gly Pro 
                805     


<210>  9
<211>  2418
<212>  DNA
<213>  Homo sapiens

<400>  9
atggcgaaaa gcagcagcct gaacgtgcgc gtggtggaag gccgcgcgct gccggcgaaa       60

gatgtgagcg gcagcagcga tccgtattgc ctggtgaaag tggatgatga agtggtggcg      120

cgcaccgcga ccgtgtggcg cagcctgggc ccgttttggg gcgaagaata taccgtgcat      180

ctgccgctgg attttcatca gctggcgttt tatgtgctgg atgaagatac cgtgggccat      240

gatgatatta ttggcaaaat tagcctgagc cgcgaagcga ttaccgcgga tccgcgcggc      300

attgatagct ggattaacct gagccgcgtg gatccggatg cggaagtgca gggcgaaatt      360

tgcctgagcg tgcagatgct ggaagatggc cagggccgct gcctgcgctg ccatgtgctg      420

caggcgcgcg atctggcgcc gcgcgatatt agcggcacca gcgatccgtt tgcgcgcgtg      480

ttttggggca gccagagcct ggaaaccagc accattaaaa aaacccgctt tccgcattgg      540

gatgaagtgc tggaactgcg cgaaatgccg ggcgcgccga gcccgctgcg cgtggaactg      600

tgggattggg atatggtggg caaaaacgat tttctgggca tggtggaatt tagcccgaaa      660

accctgcagc agaaaccgcc gaaaggctgg tttcgcctgc tgccgtttcc gcgcgcggaa      720

gaagatagcg gcggcaacct gggcgcgctg cgcgtgaaag tgcgcctgat tgaagatcgc      780

gtgctgccga gccagtgcta tcagccgctg atggaactgc tgatggaaag cgtgcagggc      840

ccggcggaag aagataccgc gagcccgctg gcgctgctgg aagaactgac cctgggcgat      900

tgccgccagg atctggcgac caaactggtg aaactgtttc tgggccgcgg cctggcgggc      960

cgctttctgg attatctgac ccgccgcgaa gtggcgcgca ccatggatcc gaacaccctg     1020

tttcgcagca acagcctggc gagcaaaagc atggaacagt ttatgaaact ggtgggcatg     1080

ccgtatctgc atgaagtgct gaaaccggtg attagccgcg tgtttgaaga aaaaaaatat     1140

atggaactgg atccgtgcaa aatggatctg ggccgcaccc gccgcattag ctttaaaggc     1200

gcgctgagcg aagaacagat gcgcgaaacc agcctgggcc tgctgaccgg ctatctgggc     1260

ccgattgtgg atgcgattgt gggcagcgtg ggccgctgcc cgccggcgat gcgcctggcg     1320

tttaaacagc tgcatcgccg cgtggaagaa cgctttccgc aggcggaaca tcagcaggat     1380

gtgaaatatc tggcgattag cggctttctg tttctgcgct tttttgcgcc ggcgattctg     1440

accccgaaac tgtttgatct gcgcgatcag catgcggatc cgcagaccag ccgcagcctg     1500

ctgctgctgg cgaaagcggt gcagagcatt ggcaacctgg gccagcagct gggccagggc     1560

aaagaactgt ggatggcgcc gctgcatccg tttctgctgc agtgcgtgag ccgcgtgcgc     1620

gattttctgg atcgcctggt ggatgtggat ggcgatgaag aagcgggcgt gccggcgcgc     1680

gcgctgtttc cgccgagcgc gattgtgcgc gaaggctatc tgctgaaacg caaagaagaa     1740

ccggcgggcc tggcgacccg ctttgcgttt aaaaaacgct atgtgtggct gagcggcgaa     1800

accctgagct ttagcaaaag cccggaatgg cagatgtgcc atagcattcc ggtgagccat     1860

attcgcgcgg tggaacgcgt ggatgaaggc gcgtttcagc tgccgcatgt gatgcaggtg     1920

gtgacccagg atggcaccgg cgcgctgcat accacctatc tgcagtgcaa aaacgtgaac     1980

gaactgaacc agtggctgag cgcgctgcgc aaagcgagcg cgccgaaccc gaacaaactg     2040

gcggcgtgcc atccgggcgc gtttcgcagc gcgcgctgga cctgctgcct gcaggcggaa     2100

cgcagcgcgg cgggctgcag ccgcacccat agcgcggtga ccctgggcga ttggagcgat     2160

ccgctggatc cggatgcgga agcgcagacc gtgtatcgcc agctgctgct gggccgcgat     2220

cagctgcgcc tgaaactgct ggaagatagc aacatggata ccaccctgga agcggatacc     2280

ggcgcgtgcc cggaagtgct ggcgcgccag cgcgcggcga ccgcgcgcct gctggaagtg     2340

ctggcggatc tggatcgcgc gcatgaagaa tttcagcagc aggaacgcgg caaagcggcg     2400

ctgggcccgc tgggcccg                                                   2418


<210>  10
<211>  805
<212>  PRT
<213>  Homo sapiens Isoform 4 of RasGAP-activating-like protein 1

<400>  10

Met Ala Lys Ser Ser Ser Leu Asn Val Arg Val Val Glu Gly Arg Ala 
1               5                   10                  15      


Leu Pro Ala Lys Asp Val Ser Gly Ser Ser Asp Pro Tyr Cys Leu Val 
            20                  25                  30          


Lys Val Asp Asp Glu Val Val Ala Arg Thr Ala Thr Val Trp Arg Ser 
        35                  40                  45              


Leu Gly Pro Phe Trp Gly Glu Glu Tyr Thr Val His Leu Pro Leu Asp 
    50                  55                  60                  


Phe His Gln Leu Ala Phe Tyr Val Leu Asp Glu Asp Thr Val Gly His 
65                  70                  75                  80  


Asp Asp Ile Ile Gly Lys Ile Ser Leu Ser Arg Glu Ala Ile Thr Ala 
                85                  90                  95      


Asp Pro Arg Gly Ile Asp Ser Trp Ile Asn Leu Ser Arg Val Asp Pro 
            100                 105                 110         


Asp Ala Glu Val Gln Gly Glu Ile Cys Leu Ser Val Gln Met Leu Glu 
        115                 120                 125             


Asp Gly Gln Gly Arg Cys Leu Arg Cys His Val Leu Gln Ala Arg Asp 
    130                 135                 140                 


Leu Ala Pro Arg Asp Ile Ser Gly Thr Ser Asp Pro Phe Ala Arg Val 
145                 150                 155                 160 


Phe Trp Gly Ser Gln Ser Leu Glu Thr Ser Thr Ile Lys Lys Thr Arg 
                165                 170                 175     


Phe Pro His Trp Asp Glu Val Leu Glu Leu Arg Glu Met Pro Gly Ala 
            180                 185                 190         


Pro Ser Pro Leu Arg Val Glu Leu Trp Asp Trp Asp Met Val Gly Lys 
        195                 200                 205             


Asn Asp Phe Leu Gly Met Val Glu Phe Ser Pro Lys Thr Leu Gln Gln 
    210                 215                 220                 


Lys Pro Pro Lys Gly Trp Phe Arg Leu Leu Pro Phe Pro Arg Ala Glu 
225                 230                 235                 240 


Glu Asp Ser Gly Gly Asn Leu Gly Ala Leu Arg Val Lys Val Arg Leu 
                245                 250                 255     


Ile Glu Asp Arg Val Leu Pro Ser Gln Cys Tyr Gln Pro Leu Met Glu 
            260                 265                 270         


Leu Leu Met Glu Ser Val Gln Gly Pro Ala Glu Glu Asp Thr Ala Ser 
        275                 280                 285             


Pro Leu Ala Leu Leu Glu Glu Leu Thr Leu Gly Asp Cys Arg Gln Asp 
    290                 295                 300                 


Leu Ala Thr Lys Leu Val Lys Leu Phe Leu Gly Arg Gly Leu Ala Gly 
305                 310                 315                 320 


Arg Phe Leu Asp Tyr Leu Thr Arg Arg Glu Val Ala Arg Thr Met Asp 
                325                 330                 335     


Pro Asn Thr Leu Phe Arg Ser Asn Ser Leu Ala Ser Lys Ser Met Glu 
            340                 345                 350         


Gln Phe Met Lys Leu Val Gly Met Pro Tyr Leu His Glu Val Leu Lys 
        355                 360                 365             


Pro Val Ile Ser Arg Val Phe Glu Glu Lys Lys Tyr Met Glu Leu Asp 
    370                 375                 380                 


Pro Cys Lys Met Asp Leu Gly Arg Thr Arg Arg Ile Ser Phe Lys Gly 
385                 390                 395                 400 


Ala Leu Ser Glu Glu Gln Met Arg Glu Thr Ser Leu Gly Leu Leu Thr 
                405                 410                 415     


Gly Tyr Leu Gly Pro Ile Val Asp Ala Ile Val Gly Ser Val Gly Arg 
            420                 425                 430         


Cys Pro Pro Ala Met Arg Leu Ala Phe Lys Gln Leu His Arg Arg Val 
        435                 440                 445             


Glu Glu Arg Phe Pro Gln Ala Glu His Gln Asp Val Lys Tyr Leu Ala 
    450                 455                 460                 


Ile Ser Gly Phe Leu Phe Leu Arg Phe Phe Ala Pro Ala Ile Leu Thr 
465                 470                 475                 480 


Pro Lys Leu Phe Asp Leu Arg Asp Gln His Ala Asp Pro Gln Thr Ser 
                485                 490                 495     


Arg Ser Leu Leu Leu Leu Ala Lys Ala Val Gln Ser Ile Gly Asn Leu 
            500                 505                 510         


Gly Gln Gln Leu Gly Gln Gly Lys Glu Leu Trp Met Ala Pro Leu His 
        515                 520                 525             


Pro Phe Leu Leu Gln Cys Val Ser Arg Val Arg Asp Phe Leu Asp Arg 
    530                 535                 540                 


Leu Val Asp Val Asp Gly Asp Glu Glu Ala Gly Val Pro Ala Arg Ala 
545                 550                 555                 560 


Leu Phe Pro Pro Ser Ala Ile Val Arg Glu Gly Tyr Leu Leu Lys Arg 
                565                 570                 575     


Lys Glu Glu Pro Ala Gly Leu Ala Thr Arg Phe Ala Phe Lys Lys Arg 
            580                 585                 590         


Tyr Val Trp Leu Ser Gly Glu Thr Leu Ser Phe Ser Lys Ser Pro Glu 
        595                 600                 605             


Trp Gln Met Cys His Ser Ile Pro Val Ser His Ile Arg Ala Val Glu 
    610                 615                 620                 


Arg Val Asp Glu Gly Ala Phe Gln Leu Pro His Val Met Gln Val Val 
625                 630                 635                 640 


Thr Gln Asp Gly Thr Gly Ala Leu His Thr Thr Tyr Leu Gln Cys Lys 
                645                 650                 655     


Asn Val Asn Glu Leu Asn Gln Trp Leu Ser Ala Leu Arg Lys Ala Ser 
            660                 665                 670         


Ala Pro Asn Pro Asn Lys Leu Ala Ala Cys His Pro Gly Ala Phe Arg 
        675                 680                 685             


Ser Ala Arg Trp Thr Cys Cys Leu Gln Ala Glu Arg Ser Ala Ala Gly 
    690                 695                 700                 


Cys Ser Arg Thr His Ser Ala Val Thr Leu Gly Asp Trp Ser Asp Pro 
705                 710                 715                 720 


Leu Asp Pro Asp Ala Glu Ala Gln Thr Val Tyr Arg Gln Leu Leu Leu 
                725                 730                 735     


Gly Arg Asp Gln Leu Arg Leu Lys Leu Leu Glu Asp Ser Asn Met Asp 
            740                 745                 750         


Thr Thr Leu Glu Ala Asp Thr Gly Ala Cys Pro Glu Val Leu Ala Arg 
        755                 760                 765             


Gln Arg Ala Ala Thr Ala Arg Leu Leu Glu Val Leu Ala Asp Leu Asp 
    770                 775                 780                 


Arg Ala His Glu Glu Phe Gln Gln Gln Glu Arg Gly Lys Ala Ala Leu 
785                 790                 795                 800 


Gly Pro Leu Gly Pro 
                805 


<210>  11
<211>  2415
<212>  DNA
<213>  Homo sapiens

<400>  11
atggcgaaaa gcagcagcct gaacgtgcgc gtggtggaag gccgcgcgct gccggcgaaa       60

gatgtgagcg gcagcagcga tccgtattgc ctggtgaaag tggatgatga agtggtggcg      120

cgcaccgcga ccgtgtggcg cagcctgggc ccgttttggg gcgaagaata taccgtgcat      180

ctgccgctgg attttcatca gctggcgttt tatgtgctgg atgaagatac cgtgggccat      240

gatgatatta ttggcaaaat tagcctgagc cgcgaagcga ttaccgcgga tccgcgcggc      300

attgatagct ggattaacct gagccgcgtg gatccggatg cggaagtgca gggcgaaatt      360

tgcctgagcg tgcagatgct ggaagatggc cagggccgct gcctgcgctg ccatgtgctg      420

caggcgcgcg atctggcgcc gcgcgatatt agcggcacca gcgatccgtt tgcgcgcgtg      480

ttttggggca gccagagcct ggaaaccagc accattaaaa aaacccgctt tccgcattgg      540

gatgaagtgc tggaactgcg cgaaatgccg ggcgcgccga gcccgctgcg cgtggaactg      600

tgggattggg atatggtggg caaaaacgat tttctgggca tggtggaatt tagcccgaaa      660

accctgcagc agaaaccgcc gaaaggctgg tttcgcctgc tgccgtttcc gcgcgcggaa      720

gaagatagcg gcggcaacct gggcgcgctg cgcgtgaaag tgcgcctgat tgaagatcgc      780

gtgctgccga gccagtgcta tcagccgctg atggaactgc tgatggaaag cgtgcagggc      840

ccggcggaag aagataccgc gagcccgctg gcgctgctgg aagaactgac cctgggcgat      900

tgccgccagg atctggcgac caaactggtg aaactgtttc tgggccgcgg cctggcgggc      960

cgctttctgg attatctgac ccgccgcgaa gtggcgcgca ccatggatcc gaacaccctg     1020

tttcgcagca acagcctggc gagcaaaagc atggaacagt ttatgaaact ggtgggcatg     1080

ccgtatctgc atgaagtgct gaaaccggtg attagccgcg tgtttgaaga aaaaaaatat     1140

atggaactgg atccgtgcaa aatggatctg ggccgcaccc gccgcattag ctttaaaggc     1200

gcgctgagcg aagaacagat gcgcgaaacc agcctgggcc tgctgaccgg ctatctgggc     1260

ccgattgtgg atgcgattgt gggcagcgtg ggccgctgcc cgccggcgat gcgcctggcg     1320

tttaaacagc tgcatcgccg cgtggaagaa cgctttccgc aggcggaaca tcaggatgtg     1380

aaatatctgg cgattagcgg ctttctgttt ctgcgctttt ttgcgccggc gattctgacc     1440

ccgaaactgt ttgatctgcg cgatcagcat gcggatccgc agaccagccg cagcctgctg     1500

ctgctggcga aagcggtgca gagcattggc aacctgggcc agcagctggg ccagggcaaa     1560

gaactgtgga tggcgccgct gcatccgttt ctgctgcagt gcgtgagccg cgtgcgcgat     1620

tttctggatc gcctggtgga tgtggatggc gatgaagaag cgggcgtgcc ggcgcgcgcg     1680

ctgtttccgc cgagcgcgat tgtgcgcgaa ggctatctgc tgaaacgcaa agaagaaccg     1740

gcgggcctgg cgacccgctt tgcgtttaaa aaacgctatg tgtggctgag cggcgaaacc     1800

ctgagcttta gcaaaagccc ggaatggcag atgtgccata gcattccggt gagccatatt     1860

cgcgcggtgg aacgcgtgga tgaaggcgcg tttcagctgc cgcatgtgat gcaggtggtg     1920

acccaggatg gcaccggcgc gctgcatacc acctatctgc agtgcaaaaa cgtgaacgaa     1980

ctgaaccagt ggctgagcgc gctgcgcaaa gcgagcgcgc cgaacccgaa caaactggcg     2040

gcgtgccatc cgggcgcgtt tcgcagcgcg cgctggacct gctgcctgca ggcggaacgc     2100

agcgcggcgg gctgcagccg cacccatagc gcggtgaccc tgggcgattg gagcgatccg     2160

ctggatccgg atgcggaagc gcagaccgtg tatcgccagc tgctgctggg ccgcgatcag     2220

ctgcgcctga aactgctgga agatagcaac atggatacca ccctggaagc ggataccggc     2280

gcgtgcccgg aagtgctggc gcgccagcgc gcggcgaccg cgcgcctgct ggaagtgctg     2340

gcggatctgg atcgcgcgca tgaagaattt cagcagcagg aacgcggcaa agcggcgctg     2400

ggcccgctgg gcccg                                                      2415


