               SEQUENCE LISTING

<110> FUNDACION PARA LA INVESTIGACION MEDICA APLICADA

<120> NUCLEIC ACID CONSTRUCTS AND GENE THERAPY VECTORS FOR USE IN THE TREATMENT OF WILSON DISEASE AND OTHER CONDITIONS

<130> 2014005EP00

<160> 21

<170> BiSSAP 1.3

<210> 1
<211> 5107
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleic acid construct of expression vector AAV2-AAT-wtATP7B

<220> 
<221> repeat_region
<222> 1..141
<223> /note="5' ITR of adeno-associated virus serotype 2"

<220> 
<221> promoter
<222> 156..460
<223> /note="alpha 1 antitrypsin"

<220> 
<221> CDS
<222> 473..4870
<223> /note="Sequence encoding ATP7B (Copper transporting ATPase 2)"
      /transl_table=1

<220> 
<221> polyA_signal
<222> 4877..4932

<220> 
<221> repeat_region
<222> 4968..5107
<223> /standard_name="3' ITR of adeno-associated virus serotype 2"

<400> 1
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc    60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca   120

actccatcac taggggttcc tgcggccgca cgcgtcgcca ccccctccac cttggacaca   180

ggacgctgtg gtttctgagc caggtacaat gactcctttc ggtaagtgca gtggaagctg   240

tacactgccc aggcaaagcg tccgggcagc gtaggcgggc gactcagatc ccagccagtg   300

gacttagccc ctgtttgctc ctccgataac tggggtgacc ttggttaata ttcaccagca   360

gcctcccccg ttgcccctct ggatccactg cttaaatacg gacgaggaca gggccctgtc   420

tcctcagctt caggcaccac cactgacctg ggacagtgaa gcggccgcca cc atg cct   478
                                                          Met Pro 
                                                          1       

gag cag gag aga cag atc aca gcc aga gaa ggg gcc agt cgg aaa atc     526
Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg Lys Ile 
        5                   10                  15              

tta tct aag ctt tct ttg cct acc cgt gcc tgg gaa cca gca atg aag     574
Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala Met Lys 
    20                  25                  30                  

aag agt ttt gct ttt gac aat gtt ggc tat gaa ggt ggt ctg gat ggc     622
Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu Asp Gly 
35                  40                  45                  50  

ctg ggc cct tct tct cag gtg gcc acc agc aca gtc agg atc ttg ggc     670
Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile Leu Gly 
                55                  60                  65      

atg act tgc cag tca tgt gtg aag tcc att gag gac agg att tcc aat     718
Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile Ser Asn 
            70                  75                  80          

ttg aaa ggc atc atc agc atg aag gtt tcc ctg gaa caa ggc agt gcc     766
Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly Ser Ala 
        85                  90                  95              

act gtg aaa tat gtg cca tcg gtt gtg tgc ctg caa cag gtt tgc cat     814
Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val Cys His 
    100                 105                 110                 

caa att ggg gac atg ggc ttc gag gcc agc att gca gaa gga aag gca     862
Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly Lys Ala 
115                 120                 125                 130 

gcc tcc tgg ccc tca agg tcc ttg cct gcc cag gag gct gtg gtc aag     910
Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val Val Lys 
                135                 140                 145     

ctc cgg gtg gag ggc atg acc tgc cag tcc tgt gtc agc tcc att gaa     958
Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser Ile Glu 
            150                 155                 160         

ggc aag gtc cgg aaa ctg caa gga gta gtg aga gtc aaa gtc tca ctc    1006
Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val Ser Leu 
        165                 170                 175             

agc aac caa gag gcc gtc atc act tat cag cct tat ctc att cag ccc    1054
Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile Gln Pro 
    180                 185                 190                 

gaa gac ctc agg gac cat gta aat gac atg gga ttt gaa gct gcc atc    1102
Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala Ala Ile 
195                 200                 205                 210 

aag agc aaa gtg gct ccc tta agc ctg gga cca att gat att gag cgg    1150
Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Asp Ile Glu Arg 
                215                 220                 225     

tta caa agc act aac cca aag aga cct tta tct tct gct aac cag aat    1198
Leu Gln Ser Thr Asn Pro Lys Arg Pro Leu Ser Ser Ala Asn Gln Asn 
            230                 235                 240         

ttt aat aat tct gag acc ttg ggg cac caa gga agc cat gtg gtc acc    1246
Phe Asn Asn Ser Glu Thr Leu Gly His Gln Gly Ser His Val Val Thr 
        245                 250                 255             

ctc caa ctg aga ata gat gga atg cat tgt aag tct tgc gtc ttg aat    1294
Leu Gln Leu Arg Ile Asp Gly Met His Cys Lys Ser Cys Val Leu Asn 
    260                 265                 270                 

att gaa gaa aat att ggc cag ctc cta ggg gtt caa agt att caa gtg    1342
Ile Glu Glu Asn Ile Gly Gln Leu Leu Gly Val Gln Ser Ile Gln Val 
275                 280                 285                 290 

tcc ttg gag aac aaa act gcc caa gta aag tat gac cct tct tgt acc    1390
Ser Leu Glu Asn Lys Thr Ala Gln Val Lys Tyr Asp Pro Ser Cys Thr 
                295                 300                 305     

agc cca gtg gct ctg cag agg gct atc gag gca ctt cca cct ggg aat    1438
Ser Pro Val Ala Leu Gln Arg Ala Ile Glu Ala Leu Pro Pro Gly Asn 
            310                 315                 320         

ttt aaa gtt tct ctt cct gat gga gcc gaa ggg agt ggg aca gat cac    1486
Phe Lys Val Ser Leu Pro Asp Gly Ala Glu Gly Ser Gly Thr Asp His 
        325                 330                 335             

agg tct tcc agt tct cat tcc cct ggc tcc cca ccg aga aac cag gtc    1534
Arg Ser Ser Ser Ser His Ser Pro Gly Ser Pro Pro Arg Asn Gln Val 
    340                 345                 350                 

cag ggc aca tgc agt acc act ctg att gcc att gcc ggc atg acc tgt    1582
Gln Gly Thr Cys Ser Thr Thr Leu Ile Ala Ile Ala Gly Met Thr Cys 
355                 360                 365                 370 

gca tcc tgt gtc cat tcc att gaa ggc atg atc tcc caa ctg gaa ggg    1630
Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu Glu Gly 
                375                 380                 385     

gtg cag caa ata tcg gtg tct ttg gcc gaa ggg act gca aca gtt ctt    1678
Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr Val Leu 
            390                 395                 400         

tat aat ccc tct gta att agc cca gaa gaa ctc aga gct gct ata gaa    1726
Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala Ile Glu 
        405                 410                 415             

gac atg gga ttt gag gct tca gtc gtt tct gaa agc tgt tct act aac    1774
Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser Thr Asn 
    420                 425                 430                 

cct ctt gga aac cac agt gct ggg aat tcc atg gtg caa act aca gat    1822
Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr Thr Asp 
435                 440                 445                 450 

ggt aca cct aca tct gtg cag gaa gtg gct ccc cac act ggg agg ctc    1870
Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly Arg Leu 
                455                 460                 465     

cct gca aac cat gcc ccg gac atc ttg gca aag tcc cca caa tca acc    1918
Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln Ser Thr 
            470                 475                 480         

aga gca gtg gca ccg cag aag tgc ttc tta cag atc aaa ggc atg acc    1966
Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly Met Thr 
        485                 490                 495             

tgt gca tcc tgt gtg tct aac ata gaa agg aat ctg cag aaa gaa gct    2014
Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys Glu Ala 
    500                 505                 510                 

ggt gtt ctc tcc gtg ttg gtt gcc ttg atg gca gga aag gca gag atc    2062
Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala Glu Ile 
515                 520                 525                 530 

aag tat gac cca gag gtc atc cag ccc ctc gag ata gct cag ttc atc    2110
Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln Phe Ile 
                535                 540                 545     

cag gac ctg ggt ttt gag gca gca gtc atg gag gac tac gca ggc tcc    2158
Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala Gly Ser 
            550                 555                 560         

gat ggc aac att gag ctg aca atc aca ggg atg acc tgc gcg tcc tgt    2206
Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala Ser Cys 
        565                 570                 575             

gtc cac aac ata gag tcc aaa ctc acg agg aca aat ggc atc act tat    2254
Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile Thr Tyr 
    580                 585                 590                 

gcc tcc gtt gcc ctt gcc acc agc aaa gcc ctt gtt aag ttt gac ccg    2302
Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe Asp Pro 
595                 600                 605                 610 

gaa att atc ggt cca cgg gat att atc aaa att att gag gaa att ggc    2350
Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu Ile Gly 
                615                 620                 625     

ttt cat gct tcc ctg gcc cag aga aac ccc aac gct cat cac ttg gac    2398
Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His Leu Asp 
            630                 635                 640         

cac aag atg gaa ata aag cag tgg aag aag tct ttc ctg tgc agc ctg    2446
His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys Ser Leu 
        645                 650                 655             

gtg ttt ggc atc cct gtc atg gcc tta atg atc tat atg ctg ata ccc    2494
Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu Ile Pro 
    660                 665                 670                 

agc aac gag ccc cac cag tcc atg gtc ctg gac cac aac atc att cca    2542
Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile Ile Pro 
675                 680                 685                 690 

gga ctg tcc att cta aat ctc atc ttc ttt atc ttg tgt acc ttt gtc    2590
Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr Phe Val 
                695                 700                 705     

cag ctc ctc ggt ggg tgg tac ttc tac gtt cag gcc tac aaa tct ctg    2638
Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys Ser Leu 
            710                 715                 720         

aga cac agg tca gcc aac atg gac gtg ctc atc gtc ctg gcc aca agc    2686
Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala Thr Ser 
        725                 730                 735             

att gct tat gtt tat tct ctg gtc atc ctg gtg gtt gct gtg gct gag    2734
Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val Ala Glu 
    740                 745                 750                 

aag gcg gag agg agc cct gtg aca ttc ttc gac acg ccc ccc atg ctc    2782
Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro Met Leu 
755                 760                 765                 770 

ttt gtg ttc att gcc ctg ggc cgg tgg ctg gaa cac ttg gca aag agc    2830
Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala Lys Ser 
                775                 780                 785     

aaa acc tca gaa gcc ctg gct aaa ctc atg tct ctc caa gcc aca gaa    2878
Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala Thr Glu 
            790                 795                 800         

gcc acc gtt gtg acc ctt ggt gag gac aat tta atc atc agg gag gag    2926
Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg Glu Glu 
        805                 810                 815             

caa gtc ccc atg gag ctg gtg cag cgg ggc gat atc gtc aag gtg gtc    2974
Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys Val Val 
    820                 825                 830                 

cct ggg gga aag ttt cca gtg gat ggg aaa gtc ctg gaa ggc aat acc    3022
Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly Asn Thr 
835                 840                 845                 850 

atg gct gat gag tcc ctc atc aca gga gaa gcc atg cca gtc act aag    3070
Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val Thr Lys 
                855                 860                 865     

aaa ccc gga agc act gta att gcg ggg tct ata aat gca cat ggc tct    3118
Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His Gly Ser 
            870                 875                 880         

gtg ctc att aaa gct acc cac gtg ggc aat gac acc act ttg gct cag    3166
Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu Ala Gln 
        885                 890                 895             

att gtg aaa ctg gtg gaa gag gct cag atg tca aag gca ccc att cag    3214
Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro Ile Gln 
    900                 905                 910                 

cag ctg gct gac cgg ttt agt gga tat ttt gtc cca ttt atc atc atc    3262
Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile Ile Ile 
915                 920                 925                 930 

atg tca act ttg acg ttg gtg gta tgg att gta atc ggt ttt atc gat    3310
Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe Ile Asp 
                935                 940                 945     

ttt ggt gtt gtt cag aga tac ttt cct aac ccc aac aag cac atc tcc    3358
Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His Ile Ser 
            950                 955                 960         

cag aca gag gtg atc atc cgg ttt gct ttc cag acg tcc atc acg gtg    3406
Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile Thr Val 
        965                 970                 975             

ctg tgc att gcc tgc ccc tgc tcc ctg ggg ctg gcc acg ccc acg gct    3454
Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro Thr Ala 
    980                 985                 990                 

gtc atg gtg ggc acc ggg gtg gcc gcg cag aac ggc atc ctc atc aag    3502
Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu Ile Lys 
995                 1000                1005                1010

gga ggc aag ccc ctg gag atg gcg cac aag ata aag act gtg atg ttt    3550
Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val Met Phe 
                1015                1020                1025    

gac aag act ggc acc att acc cat ggc gtc ccc agg gtc atg cgg gtg    3598
Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met Arg Val 
            1030                1035                1040        

ctc ctg ctg ggg gat gtg gcc aca ctg ccc ctc agg aag gtt ctg gct    3646
Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val Leu Ala 
        1045                1050                1055            

gtg gtg ggg act gcg gag gcc agc agt gaa cac ccc ttg ggc gtg gca    3694
Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly Val Ala 
    1060                1065                1070                

gtc acc aaa tac tgt aaa gag gaa ctt gga aca gag acc ttg gga tac    3742
Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu Gly Tyr 
1075                1080                1085                1090

tgc acg gac ttc cag gca gtg cca ggc tgt gga att ggg tgc aaa gtc    3790
Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys Lys Val 
                1095                1100                1105    

agc aac gtg gaa ggc atc ctg gcc cac agt gag cgc cct ttg agt gca    3838
Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu Ser Ala 
            1110                1115                1120        

ccg gcc agt cac ctg aat gag gct ggc agc ctt ccc gca gaa aaa gat    3886
Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu Lys Asp 
        1125                1130                1135            

gca gtc ccc cag acc ttc tct gtg ctg att gga aac cgt gag tgg ctg    3934
Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu Trp Leu 
    1140                1145                1150                

agg cgc aac ggt tta acc att tct agc gat gtc agt gac gct atg aca    3982
Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala Met Thr 
1155                1160                1165                1170

gac cac gag atg aaa gga cag aca gcc atc ctg gtg gct att gac ggt    4030
Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile Asp Gly 
                1175                1180                1185    

gtg ctc tgt ggg atg atc gca atc gca gac gct gtc aag cag gag gct    4078
Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln Glu Ala 
            1190                1195                1200        

gcc ctg gct gtg cac acg ctg cag agc atg ggt gtg gac gtg gtt ctg    4126
Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val Val Leu 
        1205                1210                1215            

atc acg ggg gac aac cgg aag aca gcc aga gct att gcc acc cag gtt    4174
Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr Gln Val 
    1220                1225                1230                

ggc atc aac aaa gtc ttt gca gag gtg ctg cct tcg cac aag gtg gcc    4222
Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys Val Ala 
1235                1240                1245                1250

aag gtc cag gag ctc cag aat aaa ggg aag aaa gtc gcc atg gtg ggg    4270
Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met Val Gly 
                1255                1260                1265    

gat ggg gtc aat gac tcc ccg gcc ttg gcc cag gca gac atg ggt gtg    4318
Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met Gly Val 
            1270                1275                1280        

gcc att ggc acc ggc acg gat gtg gcc atc gag gca gcc gac gtc gtc    4366
Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp Val Val 
        1285                1290                1295            

ctt atc aga aat gat ttg ctg gat gtg gtg gct agc att cac ctt tcc    4414
Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His Leu Ser 
    1300                1305                1310                

aag agg act gtc cga agg ata cgc atc aac ctg gtc ctg gca ctg att    4462
Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala Leu Ile 
1315                1320                1325                1330

tat aac ctg gtt ggg ata ccc att gca gca ggt gtc ttc atg ccc atc    4510
Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met Pro Ile 
                1335                1340                1345    

ggc att gtg ctg cag ccc tgg atg ggc tca gcg gcc atg gca gcc tcc    4558
Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala Ala Ser 
            1350                1355                1360        

tct gtg tct gtg gtg ctc tca tcc ctg cag ctc aag tgc tat aag aag    4606
Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr Lys Lys 
        1365                1370                1375            

cct gac ctg gag agg tat gag gca cag gcg cat ggc cac atg aag ccc    4654
Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met Lys Pro 
    1380                1385                1390                

ctg acg gca tcc cag gtc agt gtg cac ata ggc atg gat gac agg tgg    4702
Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp Arg Trp 
1395                1400                1405                1410

cgg gac tcc ccc agg gcc aca cca tgg gac cag gtc agc tat gtc agc    4750
Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr Val Ser 
                1415                1420                1425    

cag gtg tcg ctg tcc tcc ctg acg tcc gac aag cca tct cgg cac agc    4798
Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg His Ser 
            1430                1435                1440        

gct gca gca gac gat gat ggg gac aag tgg tct ctg ctc ctg aat ggc    4846
Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu Asn Gly 
        1445                1450                1455            

agg gat gag gag cag tac atc tga ggtaccaata aagacctctt attttcattc   4900
Arg Asp Glu Glu Gln Tyr Ile                                      
    1460                1465                                     

atcaggtgtg gttggttttt ttgtgtgggg gcggatccat cggatcccgt gcggaccgag  4960

cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc  5020

actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg  5080

agcgagcgag cgcgcagctg cctgcag                                      5107

<210> 2
<211> 1465
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:473..4870 from SEQ ID NO 1

<400> 2
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              
Asp Gly Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile 
    50                  55                  60                  
Leu Gly Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile 
65                  70                  75                  80  
Ser Asn Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly 
                85                  90                  95      
Ser Ala Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val 
            100                 105                 110         
Cys His Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly 
        115                 120                 125             
Lys Ala Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val 
    130                 135                 140                 
Val Lys Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser 
145                 150                 155                 160 
Ile Glu Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val 
                165                 170                 175     
Ser Leu Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile 
            180                 185                 190         
Gln Pro Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala 
        195                 200                 205             
Ala Ile Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Asp Ile 
    210                 215                 220                 
Glu Arg Leu Gln Ser Thr Asn Pro Lys Arg Pro Leu Ser Ser Ala Asn 
225                 230                 235                 240 
Gln Asn Phe Asn Asn Ser Glu Thr Leu Gly His Gln Gly Ser His Val 
                245                 250                 255     
Val Thr Leu Gln Leu Arg Ile Asp Gly Met His Cys Lys Ser Cys Val 
            260                 265                 270         
Leu Asn Ile Glu Glu Asn Ile Gly Gln Leu Leu Gly Val Gln Ser Ile 
        275                 280                 285             
Gln Val Ser Leu Glu Asn Lys Thr Ala Gln Val Lys Tyr Asp Pro Ser 
    290                 295                 300                 
Cys Thr Ser Pro Val Ala Leu Gln Arg Ala Ile Glu Ala Leu Pro Pro 
305                 310                 315                 320 
Gly Asn Phe Lys Val Ser Leu Pro Asp Gly Ala Glu Gly Ser Gly Thr 
                325                 330                 335     
Asp His Arg Ser Ser Ser Ser His Ser Pro Gly Ser Pro Pro Arg Asn 
            340                 345                 350         
Gln Val Gln Gly Thr Cys Ser Thr Thr Leu Ile Ala Ile Ala Gly Met 
        355                 360                 365             
Thr Cys Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu 
    370                 375                 380                 
Glu Gly Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr 
385                 390                 395                 400 
Val Leu Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala 
                405                 410                 415     
Ile Glu Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser 
            420                 425                 430         
Thr Asn Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr 
        435                 440                 445             
Thr Asp Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly 
    450                 455                 460                 
Arg Leu Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln 
465                 470                 475                 480 
Ser Thr Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly 
                485                 490                 495     
Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys 
            500                 505                 510         
Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala 
        515                 520                 525             
Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln 
    530                 535                 540                 
Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala 
545                 550                 555                 560 
Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala 
                565                 570                 575     
Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile 
            580                 585                 590         
Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe 
        595                 600                 605             
Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu 
    610                 615                 620                 
Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His 
625                 630                 635                 640 
Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys 
                645                 650                 655     
Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu 
            660                 665                 670         
Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile 
        675                 680                 685             
Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr 
    690                 695                 700                 
Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys 
705                 710                 715                 720 
Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala 
                725                 730                 735     
Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val 
            740                 745                 750         
Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro 
        755                 760                 765             
Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala 
    770                 775                 780                 
Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala 
785                 790                 795                 800 
Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg 
                805                 810                 815     
Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys 
            820                 825                 830         
Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly 
        835                 840                 845             
Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val 
    850                 855                 860                 
Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His 
865                 870                 875                 880 
Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu 
                885                 890                 895     
Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro 
            900                 905                 910         
Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile 
        915                 920                 925             
Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe 
    930                 935                 940                 
Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His 
945                 950                 955                 960 
Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile 
                965                 970                 975     
Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro 
            980                 985                 990         
Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu 
        995                 1000                1005            
Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val 
    1010                1015                1020                
Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met 
1025                1030                1035                1040
Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val 
                1045                1050                1055    
Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly 
            1060                1065                1070        
Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu 
        1075                1080                1085            
Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys 
    1090                1095                1100                
Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu 
1105                1110                1115                1120
Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu 
                1125                1130                1135    
Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu 
            1140                1145                1150        
Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala 
        1155                1160                1165            
Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile 
    1170                1175                1180                
Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln 
1185                1190                1195                1200
Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val 
                1205                1210                1215    
Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr 
            1220                1225                1230        
Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys 
        1235                1240                1245            
Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met 
    1250                1255                1260                
Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met 
1265                1270                1275                1280
Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp 
                1285                1290                1295    
Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His 
            1300                1305                1310        
Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala 
        1315                1320                1325            
Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met 
    1330                1335                1340                
Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala 
1345                1350                1355                1360
Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr 
                1365                1370                1375    
Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met 
            1380                1385                1390        
Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp 
        1395                1400                1405            
Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr 
    1410                1415                1420                
Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg 
1425                1430                1435                1440
His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu 
                1445                1450                1455    
Asn Gly Arg Asp Glu Glu Gln Tyr Ile 
            1460                1465

<210> 3
<211> 5107
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleic acid construct of expression vector AAV2-AAT-coATP7B

<220> 
<221> repeat_region
<222> 1..141
<223> /note="5' ITR of adeno-associated virus serotype 2"

<220> 
<221> promoter
<222> 156..460
<223> /note="alpha 1 antitrypsin"

<220> 
<221> CDS
<222> 473..4870
<223> /note="Codon optimized sequence encoding ATP7B"
      /transl_table=1

<220> 
<221> polyA_signal
<222> 4877..4932

<220> 
<221> repeat_region
<222> 4968..5107
<223> /note="3' ITR of adeno-associated virus serotype 2"

<400> 3
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc    60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca   120

actccatcac taggggttcc tgcggccgca cgcgtcgcca ccccctccac cttggacaca   180

ggacgctgtg gtttctgagc caggtacaat gactcctttc ggtaagtgca gtggaagctg   240

tacactgccc aggcaaagcg tccgggcagc gtaggcgggc gactcagatc ccagccagtg   300

gacttagccc ctgtttgctc ctccgataac tggggtgacc ttggttaata ttcaccagca   360

gcctcccccg ttgcccctct ggatccactg cttaaatacg gacgaggaca gggccctgtc   420

tcctcagctt caggcaccac cactgacctg ggacagtgaa gcggccgcca cc atg cca   478
                                                          Met Pro 
                                                          1       

gaa cag gaa cgc cag atc aca gca aga gag gga gca agt cgg aaa atc     526
Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg Lys Ile 
        5                   10                  15              

ctg agc aaa ctg agc ctg cca acc aga gca tgg gaa ccc gca atg aag     574
Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala Met Lys 
    20                  25                  30                  

aaa agc ttc gcc ttt gac aac gtg gga tac gag gga ggg ctg gat gga     622
Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu Asp Gly 
35                  40                  45                  50  

ctg gga cct agc tcc cag gtg gcc acc tct aca gtc cga atc ctg ggc     670
Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile Leu Gly 
                55                  60                  65      

atg act tgc cag agt tgc gtg aaa tca att gaa gac cgg atc agt aat     718
Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile Ser Asn 
            70                  75                  80          

ctg aag gga atc att agc atg aaa gtg tcc ctg gag cag ggc tca gcc     766
Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly Ser Ala 
        85                  90                  95              

acc gtg aag tat gtc cct agc gtg gtc tgc ctg cag cag gtg tgc cac     814
Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val Cys His 
    100                 105                 110                 

cag atc ggc gat atg ggg ttc gag gcc tcc att gct gaa ggg aaa gcc     862
Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly Lys Ala 
115                 120                 125                 130 

gct tct tgg cct agc cgg tcc ctg cca gca cag gaa gca gtg gtc aag     910
Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val Val Lys 
                135                 140                 145     

ctg aga gtg gag gga atg aca tgc cag agc tgc gtg agc agt atc gaa     958
Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser Ile Glu 
            150                 155                 160         

gga aag gtc cga aaa ctg cag ggc gtg gtc cgg gtg aag gtc tct ctg    1006
Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val Ser Leu 
        165                 170                 175             

agt aac cag gag gcc gtg att acc tac cag ccc tat ctg atc cag cct    1054
Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile Gln Pro 
    180                 185                 190                 

gaa gac ctg agg gat cac gtg aat gac atg ggc ttc gag gca gcc atc    1102
Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala Ala Ile 
195                 200                 205                 210 

aag tcc aaa gtg gcc cca ctg tct ctg ggg ccc att gat atc gaa aga    1150
Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Asp Ile Glu Arg 
                215                 220                 225     

ctg cag tcc acc aac cca aag agg ccc ctg tca agc gcc aac cag aac    1198
Leu Gln Ser Thr Asn Pro Lys Arg Pro Leu Ser Ser Ala Asn Gln Asn 
            230                 235                 240         

ttc aac aat agt gag acc ctg gga cac cag ggc tca cat gtg gtc aca    1246
Phe Asn Asn Ser Glu Thr Leu Gly His Gln Gly Ser His Val Val Thr 
        245                 250                 255             

ctg cag ctg agg att gac ggc atg cac tgc aag tct tgc gtg ctg aac    1294
Leu Gln Leu Arg Ile Asp Gly Met His Cys Lys Ser Cys Val Leu Asn 
    260                 265                 270                 

att gag gaa aat atc ggc cag ctg ctg ggg gtg cag tct atc cag gtc    1342
Ile Glu Glu Asn Ile Gly Gln Leu Leu Gly Val Gln Ser Ile Gln Val 
275                 280                 285                 290 

agt ctg gag aac aag act gct cag gtg aaa tac gat cct tca tgc acc    1390
Ser Leu Glu Asn Lys Thr Ala Gln Val Lys Tyr Asp Pro Ser Cys Thr 
                295                 300                 305     

agc cca gtg gca ctg cag cgc gct atc gaa gca ctg ccc cct gga aat    1438
Ser Pro Val Ala Leu Gln Arg Ala Ile Glu Ala Leu Pro Pro Gly Asn 
            310                 315                 320         

ttc aag gtg agc ctg cct gac gga gca gag gga tcc gga acc gat cac    1486
Phe Lys Val Ser Leu Pro Asp Gly Ala Glu Gly Ser Gly Thr Asp His 
        325                 330                 335             

agg tcc tct agt tca cat tcc cca ggg tct cca cca cga aac cag gtg    1534
Arg Ser Ser Ser Ser His Ser Pro Gly Ser Pro Pro Arg Asn Gln Val 
    340                 345                 350                 

cag gga aca tgt tcc acc aca ctg att gca atc gcc ggc atg act tgc    1582
Gln Gly Thr Cys Ser Thr Thr Leu Ile Ala Ile Ala Gly Met Thr Cys 
355                 360                 365                 370 

gcc tca tgc gtg cac agc att gaa ggg atg atc tct cag ctg gag gga    1630
Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu Glu Gly 
                375                 380                 385     

gtg cag cag atc tca gtc agc ctg gcc gag ggc act gct acc gtg ctg    1678
Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr Val Leu 
            390                 395                 400         

tac aat ccc agt gtc atc tca cct gag gaa ctg cgg gct gca att gag    1726
Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala Ile Glu 
        405                 410                 415             

gac atg ggg ttc gaa gct tcc gtg gtc tcc gaa tct tgc agt acc aac    1774
Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser Thr Asn 
    420                 425                 430                 

ccc ctg ggg aat cat tcc gcc gga aac tct atg gtg cag act acc gac    1822
Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr Thr Asp 
435                 440                 445                 450 

ggg aca cct act tct gtg cag gag gtc gca cca cac aca gga cgc ctg    1870
Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly Arg Leu 
                455                 460                 465     

cca gcc aat cat gct ccc gat atc ctg gcc aaa agc ccc cag tcc act    1918
Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln Ser Thr 
            470                 475                 480         

cga gct gtg gca cct cag aag tgt ttt ctg cag atc aaa ggc atg acc    1966
Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly Met Thr 
        485                 490                 495             

tgc gcc tct tgc gtg agc aac att gag cgg aat ctg cag aag gaa gct    2014
Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys Glu Ala 
    500                 505                 510                 

ggg gtg ctg agc gtg ctg gtc gca ctg atg gcc gga aag gct gag atc    2062
Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala Glu Ile 
515                 520                 525                 530 

aag tac gac cct gaa gtg atc cag cca ctg gag att gcc cag ttc atc    2110
Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln Phe Ile 
                535                 540                 545     

cag gat ctg ggc ttt gag gcc gct gtg atg gaa gac tat gct ggg agc    2158
Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala Gly Ser 
            550                 555                 560         

gat gga aac att gaa ctg acc atc acc gga atg act tgt gcc tct tgc    2206
Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala Ser Cys 
        565                 570                 575             

gtg cac aac atc gag agt aaa ctg act aga acc aat ggg att acc tac    2254
Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile Thr Tyr 
    580                 585                 590                 

gcc agt gtg gcc ctg gct aca tca aag gct ctg gtg aaa ttc gac ccc    2302
Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe Asp Pro 
595                 600                 605                 610 

gag atc att gga cct agg gat atc att aag atc att gag gaa atc ggc    2350
Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu Ile Gly 
                615                 620                 625     

ttt cac gca agc ctg gcc cag cgc aac cca aat gcc cac cat ctg gac    2398
Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His Leu Asp 
            630                 635                 640         

cat aag atg gag atc aag cag tgg aag aaa agt ttc ctg tgc tca ctg    2446
His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys Ser Leu 
        645                 650                 655             

gtg ttt gga atc ccc gtc atg gcc ctg atg atc tac atg ctg atc cct    2494
Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu Ile Pro 
    660                 665                 670                 

agc aac gag cca cac cag tcc atg gtg ctg gat cat aac atc att cct    2542
Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile Ile Pro 
675                 680                 685                 690 

ggc ctg tcc atc ctg aat ctg att ttc ttt atc ctg tgc aca ttc gtg    2590
Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr Phe Val 
                695                 700                 705     

cag ctg ctg gga ggc tgg tac ttt tat gtg cag gca tat aaa tca ctg    2638
Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys Ser Leu 
            710                 715                 720         

cga cac cgg agc gcc aat atg gac gtg ctg att gtc ctg gca acc tct    2686
Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala Thr Ser 
        725                 730                 735             

atc gcc tac gtg tat agt ctg gtc atc ctg gtg gtc gca gtg gca gag    2734
Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val Ala Glu 
    740                 745                 750                 

aag gca gaa cgg agc cca gtg act ttc ttt gat acc cct cca atg ctg    2782
Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro Met Leu 
755                 760                 765                 770 

ttc gtg ttt atc gct ctg ggc aga tgg ctg gaa cat ctg gca aag tca    2830
Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala Lys Ser 
                775                 780                 785     

aaa acc agc gag gct ctg gca aag ctg atg agc ctg cag gct acc gaa    2878
Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala Thr Glu 
            790                 795                 800         

gca aca gtg gtc act ctg gga gag gac aac ctg atc att cgc gag gaa    2926
Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg Glu Glu 
        805                 810                 815             

cag gtg cct atg gaa ctg gtc cag cga ggc gat atc gtg aag gtg gtc    2974
Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys Val Val 
    820                 825                 830                 

cca ggg gga aaa ttc ccc gtg gac ggc aag gtc ctg gag ggg aat act    3022
Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly Asn Thr 
835                 840                 845                 850 

atg gcc gat gaa tcc ctg atc acc ggc gag gct atg cct gtg aca aag    3070
Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val Thr Lys 
                855                 860                 865     

aaa cca gga tca act gtc att gct ggc agc atc aac gca cac ggg tcc    3118
Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His Gly Ser 
            870                 875                 880         

gtg ctg atc aag gcc aca cat gtc ggg aat gac aca act ctg gct cag    3166
Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu Ala Gln 
        885                 890                 895             

att gtg aaa ctg gtc gag gaa gcc cag atg tcc aag gct cct atc cag    3214
Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro Ile Gln 
    900                 905                 910                 

cag ctg gcc gat cgg ttc tcc ggc tac ttc gtg ccc ttc atc att atc    3262
Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile Ile Ile 
915                 920                 925                 930 

atg tct aca ctg act ctg gtg gtc tgg att gtg atc gga ttc att gac    3310
Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe Ile Asp 
                935                 940                 945     

ttt ggc gtg gtc cag aga tat ttt ccc aac cct aat aag cac atc agc    3358
Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His Ile Ser 
            950                 955                 960         

cag acc gaa gtg atc atc agg ttc gca ttt cag acc agt att aca gtg    3406
Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile Thr Val 
        965                 970                 975             

ctg tgc atc gcc tgc cca tgt tca ctg ggg ctg gct acc ccc aca gca    3454
Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro Thr Ala 
    980                 985                 990                 

gtg atg gtc gga aca gga gtg gca gca cag aac gga att ctg atc aag    3502
Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu Ile Lys 
995                 1000                1005                1010

ggc ggg aaa ccc ctg gag atg gcc cac aag atc aaa act gtg atg ttt    3550
Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val Met Phe 
                1015                1020                1025    

gac aaa act ggg acc att aca cat gga gtg ccc cgc gtc atg cga gtg    3598
Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met Arg Val 
            1030                1035                1040        

ctg ctg ctg ggc gat gtg gca acc ctg cct ctg aga aag gtc ctg gca    3646
Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val Leu Ala 
        1045                1050                1055            

gtg gtc gga aca gca gag gct agc tcc gaa cac cca ctg ggg gtg gcc    3694
Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly Val Ala 
    1060                1065                1070                

gtc aca aag tac tgc aaa gag gaa ctg ggc act gag acc ctg ggg tat    3742
Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu Gly Tyr 
1075                1080                1085                1090

tgt act gac ttc cag gca gtg ccc gga tgc gga atc gga tgt aaa gtc    3790
Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys Lys Val 
                1095                1100                1105    

tct aac gtg gaa ggg att ctg gct cac agt gag cgg ccc ctg agc gca    3838
Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu Ser Ala 
            1110                1115                1120        

cct gca tcc cat ctg aat gaa gca gga agc ctg cca gca gag aag gac    3886
Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu Lys Asp 
        1125                1130                1135            

gct gtg cct cag acc ttt tcc gtc ctg atc ggc aac aga gaa tgg ctg    3934
Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu Trp Leu 
    1140                1145                1150                

cgg aga aat ggg ctg aca att tct agt gac gtg tcc gat gcc atg aca    3982
Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala Met Thr 
1155                1160                1165                1170

gat cac gag atg aaa ggc cag act gca att ctg gtg gcc atc gac gga    4030
Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile Asp Gly 
                1175                1180                1185    

gtc ctg tgc ggc atg att gct atc gca gat gcc gtg aag cag gag gct    4078
Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln Glu Ala 
            1190                1195                1200        

gca ctg gcc gtc cat acc ctg cag tct atg ggc gtg gac gtg gtc ctg    4126
Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val Val Leu 
        1205                1210                1215            

atc acc ggg gat aac cgg aaa aca gct aga gca att gcc act caa gtg    4174
Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr Gln Val 
    1220                1225                1230                

ggc atc aat aag gtg ttc gct gaa gtc ctg cct agc cac aag gtc gca    4222
Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys Val Ala 
1235                1240                1245                1250

aaa gtg cag gag ctg cag aac aag ggc aag aaa gtc gcc atg gtg gga    4270
Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met Val Gly 
                1255                1260                1265    

gac ggc gtg aat gat agc cca gct ctg gca cag gca gac atg gga gtc    4318
Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met Gly Val 
            1270                1275                1280        

gct att ggg aca gga act gac gtg gca atc gag gcc gct gat gtg gtc    4366
Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp Val Val 
        1285                1290                1295            

ctg att agg aat gac ctg ctg gat gtg gtc gct tct att cat ctg agt    4414
Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His Leu Ser 
    1300                1305                1310                

aag agg aca gtg agg cgc att cgc atc aac ctg gtg ctg gcc ctg atc    4462
Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala Leu Ile 
1315                1320                1325                1330

tac aat ctg gtg gga att cca atc gca gcc ggc gtg ttt atg cca att    4510
Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met Pro Ile 
                1335                1340                1345    

ggg atc gtc ctg cag ccc tgg atg ggc tca gct gca atg gcc gct tca    4558
Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala Ala Ser 
            1350                1355                1360        

agc gtg agc gtg gtc ctg tcc tct ctg cag ctg aaa tgc tac aag aaa    4606
Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr Lys Lys 
        1365                1370                1375            

cca gac ctg gag cgg tac gaa gct cag gca cac gga cat atg aag ccc    4654
Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met Lys Pro 
    1380                1385                1390                

ctg acc gct tcc cag gtg tct gtc cac atc ggc atg gac gat aga tgg    4702
Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp Arg Trp 
1395                1400                1405                1410

agg gac agc cca agg gcc act cca tgg gat cag gtc agt tac gtg agc    4750
Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr Val Ser 
                1415                1420                1425    

cag gtc agc ctg agt tca ctg acc agc gac aag ccc tcc cgc cat tct    4798
Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg His Ser 
            1430                1435                1440        

gca gcc gct gat gac gac ggg gac aag tgg agc ctg ctg ctg aac gga    4846
Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu Asn Gly 
        1445                1450                1455            

agg gac gaa gaa cag tat atc taa ggtaccaata aagacctctt attttcattc   4900
Arg Asp Glu Glu Gln Tyr Ile                                      
    1460                1465                                     

atcaggtgtg gttggttttt ttgtgtgggg gcggatccat cggatcccgt gcggaccgag  4960

cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc  5020

actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg  5080

agcgagcgag cgcgcagctg cctgcag                                      5107

<210> 4
<211> 1465
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:473..4870 from SEQ ID NO 3

<400> 4
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              
Asp Gly Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile 
    50                  55                  60                  
Leu Gly Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile 
65                  70                  75                  80  
Ser Asn Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly 
                85                  90                  95      
Ser Ala Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val 
            100                 105                 110         
Cys His Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly 
        115                 120                 125             
Lys Ala Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val 
    130                 135                 140                 
Val Lys Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser 
145                 150                 155                 160 
Ile Glu Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val 
                165                 170                 175     
Ser Leu Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile 
            180                 185                 190         
Gln Pro Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala 
        195                 200                 205             
Ala Ile Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Asp Ile 
    210                 215                 220                 
Glu Arg Leu Gln Ser Thr Asn Pro Lys Arg Pro Leu Ser Ser Ala Asn 
225                 230                 235                 240 
Gln Asn Phe Asn Asn Ser Glu Thr Leu Gly His Gln Gly Ser His Val 
                245                 250                 255     
Val Thr Leu Gln Leu Arg Ile Asp Gly Met His Cys Lys Ser Cys Val 
            260                 265                 270         
Leu Asn Ile Glu Glu Asn Ile Gly Gln Leu Leu Gly Val Gln Ser Ile 
        275                 280                 285             
Gln Val Ser Leu Glu Asn Lys Thr Ala Gln Val Lys Tyr Asp Pro Ser 
    290                 295                 300                 
Cys Thr Ser Pro Val Ala Leu Gln Arg Ala Ile Glu Ala Leu Pro Pro 
305                 310                 315                 320 
Gly Asn Phe Lys Val Ser Leu Pro Asp Gly Ala Glu Gly Ser Gly Thr 
                325                 330                 335     
Asp His Arg Ser Ser Ser Ser His Ser Pro Gly Ser Pro Pro Arg Asn 
            340                 345                 350         
Gln Val Gln Gly Thr Cys Ser Thr Thr Leu Ile Ala Ile Ala Gly Met 
        355                 360                 365             
Thr Cys Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu 
    370                 375                 380                 
Glu Gly Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr 
385                 390                 395                 400 
Val Leu Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala 
                405                 410                 415     
Ile Glu Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser 
            420                 425                 430         
Thr Asn Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr 
        435                 440                 445             
Thr Asp Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly 
    450                 455                 460                 
Arg Leu Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln 
465                 470                 475                 480 
Ser Thr Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly 
                485                 490                 495     
Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys 
            500                 505                 510         
Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala 
        515                 520                 525             
Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln 
    530                 535                 540                 
Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala 
545                 550                 555                 560 
Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala 
                565                 570                 575     
Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile 
            580                 585                 590         
Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe 
        595                 600                 605             
Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu 
    610                 615                 620                 
Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His 
625                 630                 635                 640 
Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys 
                645                 650                 655     
Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu 
            660                 665                 670         
Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile 
        675                 680                 685             
Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr 
    690                 695                 700                 
Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys 
705                 710                 715                 720 
Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala 
                725                 730                 735     
Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val 
            740                 745                 750         
Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro 
        755                 760                 765             
Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala 
    770                 775                 780                 
Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala 
785                 790                 795                 800 
Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg 
                805                 810                 815     
Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys 
            820                 825                 830         
Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly 
        835                 840                 845             
Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val 
    850                 855                 860                 
Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His 
865                 870                 875                 880 
Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu 
                885                 890                 895     
Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro 
            900                 905                 910         
Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile 
        915                 920                 925             
Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe 
    930                 935                 940                 
Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His 
945                 950                 955                 960 
Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile 
                965                 970                 975     
Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro 
            980                 985                 990         
Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu 
        995                 1000                1005            
Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val 
    1010                1015                1020                
Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met 
1025                1030                1035                1040
Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val 
                1045                1050                1055    
Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly 
            1060                1065                1070        
Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu 
        1075                1080                1085            
Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys 
    1090                1095                1100                
Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu 
1105                1110                1115                1120
Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu 
                1125                1130                1135    
Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu 
            1140                1145                1150        
Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala 
        1155                1160                1165            
Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile 
    1170                1175                1180                
Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln 
1185                1190                1195                1200
Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val 
                1205                1210                1215    
Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr 
            1220                1225                1230        
Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys 
        1235                1240                1245            
Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met 
    1250                1255                1260                
Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met 
1265                1270                1275                1280
Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp 
                1285                1290                1295    
Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His 
            1300                1305                1310        
Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala 
        1315                1320                1325            
Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met 
    1330                1335                1340                
Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala 
1345                1350                1355                1360
Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr 
                1365                1370                1375    
Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met 
            1380                1385                1390        
Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp 
        1395                1400                1405            
Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr 
    1410                1415                1420                
Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg 
1425                1430                1435                1440
His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu 
                1445                1450                1455    
Asn Gly Arg Asp Glu Glu Gln Tyr Ile 
            1460                1465

<210> 5
<211> 695
<212> DNA
<213> Artificial Sequence

<220> 
<223> Hybrid promoter EalbPa1AT

<220> 
<221> enhancer
<222> 1..382
<223> /note="Albumin gene enhancer"

<220> 
<221> promoter
<222> 391..695
<223> /note="Alpha 1 antitrypsin promoter"

<400> 5
ctcgaggttc ctagattaca ttacacattc tgcaagcata gcacagagca atgttctact      60

ttaattactt tcattttctt gtatcctcac agcctagaaa ataacctgcg ttacagcatc     120

cactcagtat cccttgagca tgaggtgaca ctacttaaca tagggacgag atggtacttt     180

gtgtctcctg ctctgtcagc agggcacagt acttgctgat accagggaat gtttgttctt     240

aaataccatc attccggacg tgtttgcctt ggccagtttt ccatgtacat gcagaaagaa     300

gtttggactg atcaatacag tcctctgcct ttaaagcaat aggaaaaggc caacttgtct     360

acgtttagta tgtggctgta gatctgtacc cgccaccccc tccaccttgg acacaggacg     420

ctgtggtttc tgagccaggt acaatgactc ctttcggtaa gtgcagtgga agctgtacac     480

tgcccaggca aagcgtccgg gcagcgtagg cgggcgactc agatcccagc cagtggactt     540

agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc     600

ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc     660

agcttcaggc accaccactg acctgggaca gtgaa                                695


<210> 6
<211> 3817
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleic acid construct of expression vector
      AAV2-AAT-ATP7B(d57-486)

<220> 
<221> repeat_region
<222> 1..141
<223> /note="5' ITR of adeno-associated virus serotype 2"

<220> 
<221> promoter
<222> 156..460
<223> /note="alpha 1 antitrysin"

<220> 
<221> CDS
<222> 473..3580
<223> /note="Truncated ATP7B (Copper-transporting ATPase 2)"
      /note="Sequence encoding a truncated ATP7B (Copper transporting
      ATPase 2), carrying a deletion of amino acids 57..486"
      /transl_table=1

<220> 
<221> polyA_signal
<222> 3587..3642

<220> 
<221> repeat_region
<222> 3678..3817
<223> /note="3' ITR of adeno-associated virus serotype 2"

<400> 6
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc    60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca   120

actccatcac taggggttcc tgcggccgca cgcgtcgcca ccccctccac cttggacaca   180

ggacgctgtg gtttctgagc caggtacaat gactcctttc ggtaagtgca gtggaagctg   240

tacactgccc aggcaaagcg tccgggcagc gtaggcgggc gactcagatc ccagccagtg   300

gacttagccc ctgtttgctc ctccgataac tggggtgacc ttggttaata ttcaccagca   360

gcctcccccg ttgcccctct ggatccactg cttaaatacg gacgaggaca gggccctgtc   420

tcctcagctt caggcaccac cactgacctg ggacagtgaa gcggccgcca cc atg cct   478
                                                          Met Pro 
                                                          1       

gag cag gag aga cag atc aca gcc aga gaa ggg gcc agt cgg aaa atc     526
Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg Lys Ile 
        5                   10                  15              

tta tct aag ctt tct ttg cct acc cgt gcc tgg gaa cca gca atg aag     574
Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala Met Lys 
    20                  25                  30                  

aag agt ttt gct ttt gac aat gtt ggc tat gaa ggt ggt ctg gat ggc     622
Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu Asp Gly 
35                  40                  45                  50  

ctg ggc cct tct tct cag ccg cag aag tgc ttc tta cag atc aaa ggc     670
Leu Gly Pro Ser Ser Gln Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly 
                55                  60                  65      

atg acc tgt gca tcc tgt gtg tct aac ata gaa agg aat ctg cag aaa     718
Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys 
            70                  75                  80          

gaa gct ggt gtt ctc tcc gtg ttg gtt gcc ttg atg gca gga aag gca     766
Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala 
        85                  90                  95              

gag atc aag tat gac cca gag gtc atc cag ccc ctc gag ata gct cag     814
Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln 
    100                 105                 110                 

ttc atc cag gac ctg ggt ttt gag gca gca gtc atg gag gac tac gca     862
Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala 
115                 120                 125                 130 

ggc tcc gat ggc aac att gag ctg aca atc aca ggg atg acc tgc gcg     910
Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala 
                135                 140                 145     

tcc tgt gtc cac aac ata gag tcc aaa ctc acg agg aca aat ggc atc     958
Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile 
            150                 155                 160         

act tat gcc tcc gtt gcc ctt gcc acc agc aaa gcc ctt gtt aag ttt    1006
Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe 
        165                 170                 175             

gac ccg gaa att atc ggt cca cgg gat att atc aaa att att gag gaa    1054
Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu 
    180                 185                 190                 

att ggc ttt cat gct tcc ctg gcc cag aga aac ccc aac gct cat cac    1102
Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His 
195                 200                 205                 210 

ttg gac cac aag atg gaa ata aag cag tgg aag aag tct ttc ctg tgc    1150
Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys 
                215                 220                 225     

agc ctg gtg ttt ggc atc cct gtc atg gcc tta atg atc tat atg ctg    1198
Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu 
            230                 235                 240         

ata ccc agc aac gag ccc cac cag tcc atg gtc ctg gac cac aac atc    1246
Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile 
        245                 250                 255             

att cca gga ctg tcc att cta aat ctc atc ttc ttt atc ttg tgt acc    1294
Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr 
    260                 265                 270                 

ttt gtc cag ctc ctc ggt ggg tgg tac ttc tac gtt cag gcc tac aaa    1342
Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys 
275                 280                 285                 290 

tct ctg aga cac agg tca gcc aac atg gac gtg ctc atc gtc ctg gcc    1390
Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala 
                295                 300                 305     

aca agc att gct tat gtt tat tct ctg gtc atc ctg gtg gtt gct gtg    1438
Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val 
            310                 315                 320         

gct gag aag gcg gag agg agc cct gtg aca ttc ttc gac acg ccc ccc    1486
Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro 
        325                 330                 335             

atg ctc ttt gtg ttc att gcc ctg ggc cgg tgg ctg gaa cac ttg gca    1534
Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala 
    340                 345                 350                 

aag agc aaa acc tca gaa gcc ctg gct aaa ctc atg tct ctc caa gcc    1582
Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala 
355                 360                 365                 370 

aca gaa gcc acc gtt gtg acc ctt ggt gag gac aat tta atc atc agg    1630
Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg 
                375                 380                 385     

gag gag caa gtc ccc atg gag ctg gtg cag cgg ggc gat atc gtc aag    1678
Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys 
            390                 395                 400         

gtg gtc cct ggg gga aag ttt cca gtg gat ggg aaa gtc ctg gaa ggc    1726
Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly 
        405                 410                 415             

aat acc atg gct gat gag tcc ctc atc aca gga gaa gcc atg cca gtc    1774
Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val 
    420                 425                 430                 

act aag aaa ccc gga agc act gta att gcg ggg tct ata aat gca cat    1822
Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His 
435                 440                 445                 450 

ggc tct gtg ctc att aaa gct acc cac gtg ggc aat gac acc act ttg    1870
Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu 
                455                 460                 465     

gct cag att gtg aaa ctg gtg gaa gag gct cag atg tca aag gca ccc    1918
Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro 
            470                 475                 480         

att cag cag ctg gct gac cgg ttt agt gga tat ttt gtc cca ttt atc    1966
Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile 
        485                 490                 495             

atc atc atg tca act ttg acg ttg gtg gta tgg att gta atc ggt ttt    2014
Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe 
    500                 505                 510                 

atc gat ttt ggt gtt gtt cag aga tac ttt cct aac ccc aac aag cac    2062
Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His 
515                 520                 525                 530 

atc tcc cag aca gag gtg atc atc cgg ttt gct ttc cag acg tcc atc    2110
Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile 
                535                 540                 545     

acg gtg ctg tgc att gcc tgc ccc tgc tcc ctg ggg ctg gcc acg ccc    2158
Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro 
            550                 555                 560         

acg gct gtc atg gtg ggc acc ggg gtg gcc gcg cag aac ggc atc ctc    2206
Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu 
        565                 570                 575             

atc aag gga ggc aag ccc ctg gag atg gcg cac aag ata aag act gtg    2254
Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val 
    580                 585                 590                 

atg ttt gac aag act ggc acc att acc cat ggc gtc ccc agg gtc atg    2302
Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met 
595                 600                 605                 610 

cgg gtg ctc ctg ctg ggg gat gtg gcc aca ctg ccc ctc agg aag gtt    2350
Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val 
                615                 620                 625     

ctg gct gtg gtg ggg act gcg gag gcc agc agt gaa cac ccc ttg ggc    2398
Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly 
            630                 635                 640         

gtg gca gtc acc aaa tac tgt aaa gag gaa ctt gga aca gag acc ttg    2446
Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu 
        645                 650                 655             

gga tac tgc acg gac ttc cag gca gtg cca ggc tgt gga att ggg tgc    2494
Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys 
    660                 665                 670                 

aaa gtc agc aac gtg gaa ggc atc ctg gcc cac agt gag cgc cct ttg    2542
Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu 
675                 680                 685                 690 

agt gca ccg gcc agt cac ctg aat gag gct ggc agc ctt ccc gca gaa    2590
Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu 
                695                 700                 705     

aaa gat gca gtc ccc cag acc ttc tct gtg ctg att gga aac cgt gag    2638
Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu 
            710                 715                 720         

tgg ctg agg cgc aac ggt tta acc att tct agc gat gtc agt gac gct    2686
Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala 
        725                 730                 735             

atg aca gac cac gag atg aaa gga cag aca gcc atc ctg gtg gct att    2734
Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile 
    740                 745                 750                 

gac ggt gtg ctc tgt ggg atg atc gca atc gca gac gct gtc aag cag    2782
Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln 
755                 760                 765                 770 

gag gct gcc ctg gct gtg cac acg ctg cag agc atg ggt gtg gac gtg    2830
Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val 
                775                 780                 785     

gtt ctg atc acg ggg gac aac cgg aag aca gcc aga gct att gcc acc    2878
Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr 
            790                 795                 800         

cag gtt ggc atc aac aaa gtc ttt gca gag gtg ctg cct tcg cac aag    2926
Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys 
        805                 810                 815             

gtg gcc aag gtc cag gag ctc cag aat aaa ggg aag aaa gtc gcc atg    2974
Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met 
    820                 825                 830                 

gtg ggg gat ggg gtc aat gac tcc ccg gcc ttg gcc cag gca gac atg    3022
Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met 
835                 840                 845                 850 

ggt gtg gcc att ggc acc ggc acg gat gtg gcc atc gag gca gcc gac    3070
Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp 
                855                 860                 865     

gtc gtc ctt atc aga aat gat ttg ctg gat gtg gtg gct agc att cac    3118
Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His 
            870                 875                 880         

ctt tcc aag agg act gtc cga agg ata cgc atc aac ctg gtc ctg gca    3166
Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala 
        885                 890                 895             

ctg att tat aac ctg gtt ggg ata ccc att gca gca ggt gtc ttc atg    3214
Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met 
    900                 905                 910                 

ccc atc ggc att gtg ctg cag ccc tgg atg ggc tca gcg gcc atg gca    3262
Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala 
915                 920                 925                 930 

gcc tcc tct gtg tct gtg gtg ctc tca tcc ctg cag ctc aag tgc tat    3310
Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr 
                935                 940                 945     

aag aag cct gac ctg gag agg tat gag gca cag gcg cat ggc cac atg    3358
Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met 
            950                 955                 960         

aag ccc ctg acg gca tcc cag gtc agt gtg cac ata ggc atg gat gac    3406
Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp 
        965                 970                 975             

agg tgg cgg gac tcc ccc agg gcc aca cca tgg gac cag gtc agc tat    3454
Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr 
    980                 985                 990                 

gtc agc cag gtg tcg ctg tcc tcc ctg acg tcc gac aag cca tct cgg    3502
Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg 
995                 1000                1005                1010

cac agc gct gca gca gac gat gat ggg gac aag tgg tct ctg ctc ctg    3550
His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu 
                1015                1020                1025    

aat ggc agg gat gag gag cag tac atc tga ggtaccaata aagacctctt      3600
Asn Gly Arg Asp Glu Glu Gln Tyr Ile                           
            1030                1035                          

attttcattc atcaggtgtg gttggttttt ttgtgtgggg gcggatccat cggatcccgt  3660

gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc  3720

tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc  3780

ggcctcagtg agcgagcgag cgcgcagctg cctgcag                           3817

<210> 7
<211> 1035
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:473..3580 from SEQ ID NO 6

<400> 7
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              
Asp Gly Leu Gly Pro Ser Ser Gln Pro Gln Lys Cys Phe Leu Gln Ile 
    50                  55                  60                  
Lys Gly Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu 
65                  70                  75                  80  
Gln Lys Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly 
                85                  90                  95      
Lys Ala Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile 
            100                 105                 110         
Ala Gln Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp 
        115                 120                 125             
Tyr Ala Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr 
    130                 135                 140                 
Cys Ala Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn 
145                 150                 155                 160 
Gly Ile Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val 
                165                 170                 175     
Lys Phe Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile 
            180                 185                 190         
Glu Glu Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala 
        195                 200                 205             
His His Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe 
    210                 215                 220                 
Leu Cys Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr 
225                 230                 235                 240 
Met Leu Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His 
                245                 250                 255     
Asn Ile Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu 
            260                 265                 270         
Cys Thr Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala 
        275                 280                 285             
Tyr Lys Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val 
    290                 295                 300                 
Leu Ala Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val 
305                 310                 315                 320 
Ala Val Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr 
                325                 330                 335     
Pro Pro Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His 
            340                 345                 350         
Leu Ala Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu 
        355                 360                 365             
Gln Ala Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile 
    370                 375                 380                 
Ile Arg Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile 
385                 390                 395                 400 
Val Lys Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu 
                405                 410                 415     
Glu Gly Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met 
            420                 425                 430         
Pro Val Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn 
        435                 440                 445             
Ala His Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr 
    450                 455                 460                 
Thr Leu Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys 
465                 470                 475                 480 
Ala Pro Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro 
                485                 490                 495     
Phe Ile Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile 
            500                 505                 510         
Gly Phe Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn 
        515                 520                 525             
Lys His Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr 
    530                 535                 540                 
Ser Ile Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala 
545                 550                 555                 560 
Thr Pro Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly 
                565                 570                 575     
Ile Leu Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys 
            580                 585                 590         
Thr Val Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg 
        595                 600                 605             
Val Met Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg 
    610                 615                 620                 
Lys Val Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro 
625                 630                 635                 640 
Leu Gly Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu 
                645                 650                 655     
Thr Leu Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile 
            660                 665                 670         
Gly Cys Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg 
        675                 680                 685             
Pro Leu Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro 
    690                 695                 700                 
Ala Glu Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn 
705                 710                 715                 720 
Arg Glu Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser 
                725                 730                 735     
Asp Ala Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val 
            740                 745                 750         
Ala Ile Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val 
        755                 760                 765             
Lys Gln Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val 
    770                 775                 780                 
Asp Val Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile 
785                 790                 795                 800 
Ala Thr Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser 
                805                 810                 815     
His Lys Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val 
            820                 825                 830         
Ala Met Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala 
        835                 840                 845             
Asp Met Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala 
    850                 855                 860                 
Ala Asp Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser 
865                 870                 875                 880 
Ile His Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val 
                885                 890                 895     
Leu Ala Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val 
            900                 905                 910         
Phe Met Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala 
        915                 920                 925             
Met Ala Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys 
    930                 935                 940                 
Cys Tyr Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly 
945                 950                 955                 960 
His Met Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met 
                965                 970                 975     
Asp Asp Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val 
            980                 985                 990         
Ser Tyr Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro 
        995                 1000                1005            
Ser Arg His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu 
    1010                1015                1020                
Leu Leu Asn Gly Arg Asp Glu Glu Gln Tyr Ile 
1025                1030                1035

<210> 8
<211> 3108
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon optimized sequence coATP7B(d57-486) encoding truncated
      ATP7B, with deletion of amino acids 57-486

<220> 
<221> CDS
<222> 1..3108
<223> /transl_table=1

<400> 8
atg cca gaa cag gaa cgc cag atc aca gca aga gag gga gca agt cgg      48
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      

aaa atc ctg agc aaa ctg agc ctg cca acc aga gca tgg gaa ccc gca      96
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          

atg aag aaa agc ttc gcc ttt gac aac gtg gga tac gag gga ggg ctg     144
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              

gat gga ctg gga cct agc tcc cag cct cag aag tgt ttt ctg cag atc     192
Asp Gly Leu Gly Pro Ser Ser Gln Pro Gln Lys Cys Phe Leu Gln Ile 
    50                  55                  60                  

aaa ggc atg acc tgc gcc tct tgc gtg agc aac att gag cgg aat ctg     240
Lys Gly Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu 
65                  70                  75                  80  

cag aag gaa gct ggg gtg ctg agc gtg ctg gtc gca ctg atg gcc gga     288
Gln Lys Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly 
                85                  90                  95      

aag gct gag atc aag tac gac cct gaa gtg atc cag cca ctg gag att     336
Lys Ala Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile 
            100                 105                 110         

gcc cag ttc atc cag gat ctg ggc ttt gag gcc gct gtg atg gaa gac     384
Ala Gln Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp 
        115                 120                 125             

tat gct ggg agc gat gga aac att gaa ctg acc atc acc gga atg act     432
Tyr Ala Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr 
    130                 135                 140                 

tgt gcc tct tgc gtg cac aac atc gag agt aaa ctg act aga acc aat     480
Cys Ala Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn 
145                 150                 155                 160 

ggg att acc tac gcc agt gtg gcc ctg gct aca tca aag gct ctg gtg     528
Gly Ile Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val 
                165                 170                 175     

aaa ttc gac ccc gag atc att gga cct agg gat atc att aag atc att     576
Lys Phe Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile 
            180                 185                 190         

gag gaa atc ggc ttt cac gca agc ctg gcc cag cgc aac cca aat gcc     624
Glu Glu Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala 
        195                 200                 205             

cac cat ctg gac cat aag atg gag atc aag cag tgg aag aaa agt ttc     672
His His Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe 
    210                 215                 220                 

ctg tgc tca ctg gtg ttt gga atc ccc gtc atg gcc ctg atg atc tac     720
Leu Cys Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr 
225                 230                 235                 240 

atg ctg atc cct agc aac gag cca cac cag tcc atg gtg ctg gat cat     768
Met Leu Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His 
                245                 250                 255     

aac atc att cct ggc ctg tcc atc ctg aat ctg att ttc ttt atc ctg     816
Asn Ile Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu 
            260                 265                 270         

tgc aca ttc gtg cag ctg ctg gga ggc tgg tac ttt tat gtg cag gca     864
Cys Thr Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala 
        275                 280                 285             

tat aaa tca ctg cga cac cgg agc gcc aat atg gac gtg ctg att gtc     912
Tyr Lys Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val 
    290                 295                 300                 

ctg gca acc tct atc gcc tac gtg tat agt ctg gtc atc ctg gtg gtc     960
Leu Ala Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val 
305                 310                 315                 320 

gca gtg gca gag aag gca gaa cgg agc cca gtg act ttc ttt gat acc    1008
Ala Val Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr 
                325                 330                 335     

cct cca atg ctg ttc gtg ttt atc gct ctg ggc aga tgg ctg gaa cat    1056
Pro Pro Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His 
            340                 345                 350         

ctg gca aag tca aaa acc agc gag gct ctg gca aag ctg atg agc ctg    1104
Leu Ala Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu 
        355                 360                 365             

cag gct acc gaa gca aca gtg gtc act ctg gga gag gac aac ctg atc    1152
Gln Ala Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile 
    370                 375                 380                 

att cgc gag gaa cag gtg cct atg gaa ctg gtc cag cga ggc gat atc    1200
Ile Arg Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile 
385                 390                 395                 400 

gtg aag gtg gtc cca ggg gga aaa ttc ccc gtg gac ggc aag gtc ctg    1248
Val Lys Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu 
                405                 410                 415     

gag ggg aat act atg gcc gat gaa tcc ctg atc acc ggc gag gct atg    1296
Glu Gly Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met 
            420                 425                 430         

cct gtg aca aag aaa cca gga tca act gtc att gct ggc agc atc aac    1344
Pro Val Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn 
        435                 440                 445             

gca cac ggg tcc gtg ctg atc aag gcc aca cat gtc ggg aat gac aca    1392
Ala His Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr 
    450                 455                 460                 

act ctg gct cag att gtg aaa ctg gtc gag gaa gcc cag atg tcc aag    1440
Thr Leu Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys 
465                 470                 475                 480 

gct cct atc cag cag ctg gcc gat cgg ttc tcc ggc tac ttc gtg ccc    1488
Ala Pro Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro 
                485                 490                 495     

ttc atc att atc atg tct aca ctg act ctg gtg gtc tgg att gtg atc    1536
Phe Ile Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile 
            500                 505                 510         

gga ttc att gac ttt ggc gtg gtc cag aga tat ttt ccc aac cct aat    1584
Gly Phe Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn 
        515                 520                 525             

aag cac atc agc cag acc gaa gtg atc atc agg ttc gca ttt cag acc    1632
Lys His Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr 
    530                 535                 540                 

agt att aca gtg ctg tgc atc gcc tgc cca tgt tca ctg ggg ctg gct    1680
Ser Ile Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala 
545                 550                 555                 560 

acc ccc aca gca gtg atg gtc gga aca gga gtg gca gca cag aac gga    1728
Thr Pro Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly 
                565                 570                 575     

att ctg atc aag ggc ggg aaa ccc ctg gag atg gcc cac aag atc aaa    1776
Ile Leu Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys 
            580                 585                 590         

act gtg atg ttt gac aaa act ggg acc att aca cat gga gtg ccc cgc    1824
Thr Val Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg 
        595                 600                 605             

gtc atg cga gtg ctg ctg ctg ggc gat gtg gca acc ctg cct ctg aga    1872
Val Met Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg 
    610                 615                 620                 

aag gtc ctg gca gtg gtc gga aca gca gag gct agc tcc gaa cac cca    1920
Lys Val Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro 
625                 630                 635                 640 

ctg ggg gtg gcc gtc aca aag tac tgc aaa gag gaa ctg ggc act gag    1968
Leu Gly Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu 
                645                 650                 655     

acc ctg ggg tat tgt act gac ttc cag gca gtg ccc gga tgc gga atc    2016
Thr Leu Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile 
            660                 665                 670         

gga tgt aaa gtc tct aac gtg gaa ggg att ctg gct cac agt gag cgg    2064
Gly Cys Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg 
        675                 680                 685             

ccc ctg agc gca cct gca tcc cat ctg aat gaa gca gga agc ctg cca    2112
Pro Leu Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro 
    690                 695                 700                 

gca gag aag gac gct gtg cct cag acc ttt tcc gtc ctg atc ggc aac    2160
Ala Glu Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn 
705                 710                 715                 720 

aga gaa tgg ctg cgg aga aat ggg ctg aca att tct agt gac gtg tcc    2208
Arg Glu Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser 
                725                 730                 735     

gat gcc atg aca gat cac gag atg aaa ggc cag act gca att ctg gtg    2256
Asp Ala Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val 
            740                 745                 750         

gcc atc gac gga gtc ctg tgc ggc atg att gct atc gca gat gcc gtg    2304
Ala Ile Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val 
        755                 760                 765             

aag cag gag gct gca ctg gcc gtc cat acc ctg cag tct atg ggc gtg    2352
Lys Gln Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val 
    770                 775                 780                 

gac gtg gtc ctg atc acc ggg gat aac cgg aaa aca gct aga gca att    2400
Asp Val Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile 
785                 790                 795                 800 

gcc act caa gtg ggc atc aat aag gtg ttc gct gaa gtc ctg cct agc    2448
Ala Thr Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser 
                805                 810                 815     

cac aag gtc gca aaa gtg cag gag ctg cag aac aag ggc aag aaa gtc    2496
His Lys Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val 
            820                 825                 830         

gcc atg gtg gga gac ggc gtg aat gat agc cca gct ctg gca cag gca    2544
Ala Met Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala 
        835                 840                 845             

gac atg gga gtc gct att ggg aca gga act gac gtg gca atc gag gcc    2592
Asp Met Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala 
    850                 855                 860                 

gct gat gtg gtc ctg att agg aat gac ctg ctg gat gtg gtc gct tct    2640
Ala Asp Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser 
865                 870                 875                 880 

att cat ctg agt aag agg aca gtg agg cgc att cgc atc aac ctg gtg    2688
Ile His Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val 
                885                 890                 895     

ctg gcc ctg atc tac aat ctg gtg gga att cca atc gca gcc ggc gtg    2736
Leu Ala Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val 
            900                 905                 910         

ttt atg cca att ggg atc gtc ctg cag ccc tgg atg ggc tca gct gca    2784
Phe Met Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala 
        915                 920                 925             

atg gcc gct tca agc gtg agc gtg gtc ctg tcc tct ctg cag ctg aaa    2832
Met Ala Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys 
    930                 935                 940                 

tgc tac aag aaa cca gac ctg gag cgg tac gaa gct cag gca cac gga    2880
Cys Tyr Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly 
945                 950                 955                 960 

cat atg aag ccc ctg acc gct tcc cag gtg tct gtc cac atc ggc atg    2928
His Met Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met 
                965                 970                 975     

gac gat aga tgg agg gac agc cca agg gcc act cca tgg gat cag gtc    2976
Asp Asp Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val 
            980                 985                 990         

agt tac gtg agc cag gtc agc ctg agt tca ctg acc agc gac aag ccc    3024
Ser Tyr Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro 
        995                 1000                1005            

tcc cgc cat tct gca gcc gct gat gac gac ggg gac aag tgg agc ctg    3072
Ser Arg His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu 
    1010                1015                1020                

ctg ctg aac gga agg gac gaa gaa cag tat atc taa                    3108
Leu Leu Asn Gly Arg Asp Glu Glu Gln Tyr Ile     
1025                1030                1035     

<210> 9
<211> 1035
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..3108 from SEQ ID NO 8

<400> 9
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              
Asp Gly Leu Gly Pro Ser Ser Gln Pro Gln Lys Cys Phe Leu Gln Ile 
    50                  55                  60                  
Lys Gly Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu 
65                  70                  75                  80  
Gln Lys Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly 
                85                  90                  95      
Lys Ala Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile 
            100                 105                 110         
Ala Gln Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp 
        115                 120                 125             
Tyr Ala Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr 
    130                 135                 140                 
Cys Ala Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn 
145                 150                 155                 160 
Gly Ile Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val 
                165                 170                 175     
Lys Phe Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile 
            180                 185                 190         
Glu Glu Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala 
        195                 200                 205             
His His Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe 
    210                 215                 220                 
Leu Cys Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr 
225                 230                 235                 240 
Met Leu Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His 
                245                 250                 255     
Asn Ile Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu 
            260                 265                 270         
Cys Thr Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala 
        275                 280                 285             
Tyr Lys Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val 
    290                 295                 300                 
Leu Ala Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val 
305                 310                 315                 320 
Ala Val Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr 
                325                 330                 335     
Pro Pro Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His 
            340                 345                 350         
Leu Ala Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu 
        355                 360                 365             
Gln Ala Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile 
    370                 375                 380                 
Ile Arg Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile 
385                 390                 395                 400 
Val Lys Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu 
                405                 410                 415     
Glu Gly Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met 
            420                 425                 430         
Pro Val Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn 
        435                 440                 445             
Ala His Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr 
    450                 455                 460                 
Thr Leu Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys 
465                 470                 475                 480 
Ala Pro Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro 
                485                 490                 495     
Phe Ile Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile 
            500                 505                 510         
Gly Phe Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn 
        515                 520                 525             
Lys His Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr 
    530                 535                 540                 
Ser Ile Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala 
545                 550                 555                 560 
Thr Pro Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly 
                565                 570                 575     
Ile Leu Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys 
            580                 585                 590         
Thr Val Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg 
        595                 600                 605             
Val Met Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg 
    610                 615                 620                 
Lys Val Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro 
625                 630                 635                 640 
Leu Gly Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu 
                645                 650                 655     
Thr Leu Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile 
            660                 665                 670         
Gly Cys Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg 
        675                 680                 685             
Pro Leu Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro 
    690                 695                 700                 
Ala Glu Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn 
705                 710                 715                 720 
Arg Glu Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser 
                725                 730                 735     
Asp Ala Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val 
            740                 745                 750         
Ala Ile Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val 
        755                 760                 765             
Lys Gln Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val 
    770                 775                 780                 
Asp Val Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile 
785                 790                 795                 800 
Ala Thr Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser 
                805                 810                 815     
His Lys Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val 
            820                 825                 830         
Ala Met Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala 
        835                 840                 845             
Asp Met Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala 
    850                 855                 860                 
Ala Asp Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser 
865                 870                 875                 880 
Ile His Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val 
                885                 890                 895     
Leu Ala Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val 
            900                 905                 910         
Phe Met Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala 
        915                 920                 925             
Met Ala Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys 
    930                 935                 940                 
Cys Tyr Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly 
945                 950                 955                 960 
His Met Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met 
                965                 970                 975     
Asp Asp Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val 
            980                 985                 990         
Ser Tyr Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro 
        995                 1000                1005            
Ser Arg His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu 
    1010                1015                1020                
Leu Leu Asn Gly Arg Asp Glu Glu Gln Tyr Ile 
1025                1030                1035

<210> 10
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer AAT-Forward

<400> 10
ctggtctaga acgcgtcgcc accccctcca ccttgg                               36


<210> 11
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer AAT-Reverse

<400> 11
atcatgatgc ggccgcttca ctgtcccagg tcagtg                               36


<210> 12
<211> 3966
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sequence encoding truncated ATP7B(d223-366) that encodes ATP7B
      with a deletion of amino acids 223..366

<220> 
<221> CDS
<222> 1..3966
<223> /transl_table=1

<400> 12
atg cct gag cag gag aga cag atc aca gcc aga gaa ggg gcc agt cgg      48
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      

aaa atc tta tct aag ctt tct ttg cct acc cgt gcc tgg gaa cca gca      96
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          

atg aag aag agt ttt gct ttt gac aat gtt ggc tat gaa ggt ggt ctg     144
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              

gat ggc ctg ggc cct tct tct cag gtg gcc acc agc aca gtc agg atc     192
Asp Gly Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile 
    50                  55                  60                  

ttg ggc atg act tgc cag tca tgt gtg aag tcc att gag gac agg att     240
Leu Gly Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile 
65                  70                  75                  80  

tcc aat ttg aaa ggc atc atc agc atg aag gtt tcc ctg gaa caa ggc     288
Ser Asn Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly 
                85                  90                  95      

agt gcc act gtg aaa tat gtg cca tcg gtt gtg tgc ctg caa cag gtt     336
Ser Ala Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val 
            100                 105                 110         

tgc cat caa att ggg gac atg ggc ttc gag gcc agc att gca gaa gga     384
Cys His Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly 
        115                 120                 125             

aag gca gcc tcc tgg ccc tca agg tcc ttg cct gcc cag gag gct gtg     432
Lys Ala Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val 
    130                 135                 140                 

gtc aag ctc cgg gtg gag ggc atg acc tgc cag tcc tgt gtc agc tcc     480
Val Lys Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser 
145                 150                 155                 160 

att gaa ggc aag gtc cgg aaa ctg caa gga gta gtg aga gtc aaa gtc     528
Ile Glu Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val 
                165                 170                 175     

tca ctc agc aac caa gag gcc gtc atc act tat cag cct tat ctc att     576
Ser Leu Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile 
            180                 185                 190         

cag ccc gaa gac ctc agg gac cat gta aat gac atg gga ttt gaa gct     624
Gln Pro Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala 
        195                 200                 205             

gcc atc aag agc aaa gtg gct ccc tta agc ctg gga cca att ggc atg     672
Ala Ile Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Gly Met 
    210                 215                 220                 

acc tgt gca tcc tgt gtc cat tcc att gaa ggc atg atc tcc caa ctg     720
Thr Cys Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu 
225                 230                 235                 240 

gaa ggg gtg cag caa ata tcg gtg tct ttg gcc gaa ggg act gca aca     768
Glu Gly Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr 
                245                 250                 255     

gtt ctt tat aat ccc tct gta att agc cca gaa gaa ctc aga gct gct     816
Val Leu Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala 
            260                 265                 270         

ata gaa gac atg gga ttt gag gct tca gtc gtt tct gaa agc tgt tct     864
Ile Glu Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser 
        275                 280                 285             

act aac cct ctt gga aac cac agt gct ggg aat tcc atg gtg caa act     912
Thr Asn Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr 
    290                 295                 300                 

aca gat ggt aca cct aca tct gtg cag gaa gtg gct ccc cac act ggg     960
Thr Asp Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly 
305                 310                 315                 320 

agg ctc cct gca aac cat gcc ccg gac atc ttg gca aag tcc cca caa    1008
Arg Leu Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln 
                325                 330                 335     

tca acc aga gca gtg gca ccg cag aag tgc ttc tta cag atc aaa ggc    1056
Ser Thr Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly 
            340                 345                 350         

atg acc tgt gca tcc tgt gtg tct aac ata gaa agg aat ctg cag aaa    1104
Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys 
        355                 360                 365             

gaa gct ggt gtt ctc tcc gtg ttg gtt gcc ttg atg gca gga aag gca    1152
Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala 
    370                 375                 380                 

gag atc aag tat gac cca gag gtc atc cag ccc ctc gag ata gct cag    1200
Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln 
385                 390                 395                 400 

ttc atc cag gac ctg ggt ttt gag gca gca gtc atg gag gac tac gca    1248
Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala 
                405                 410                 415     

ggc tcc gat ggc aac att gag ctg aca atc aca ggg atg acc tgc gcg    1296
Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala 
            420                 425                 430         

tcc tgt gtc cac aac ata gag tcc aaa ctc acg agg aca aat ggc atc    1344
Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile 
        435                 440                 445             

act tat gcc tcc gtt gcc ctt gcc acc agc aaa gcc ctt gtt aag ttt    1392
Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe 
    450                 455                 460                 

gac ccg gaa att atc ggt cca cgg gat att atc aaa att att gag gaa    1440
Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu 
465                 470                 475                 480 

att ggc ttt cat gct tcc ctg gcc cag aga aac ccc aac gct cat cac    1488
Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His 
                485                 490                 495     

ttg gac cac aag atg gaa ata aag cag tgg aag aag tct ttc ctg tgc    1536
Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys 
            500                 505                 510         

agc ctg gtg ttt ggc atc cct gtc atg gcc tta atg atc tat atg ctg    1584
Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu 
        515                 520                 525             

ata ccc agc aac gag ccc cac cag tcc atg gtc ctg gac cac aac atc    1632
Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile 
    530                 535                 540                 

att cca gga ctg tcc att cta aat ctc atc ttc ttt atc ttg tgt acc    1680
Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr 
545                 550                 555                 560 

ttt gtc cag ctc ctc ggt ggg tgg tac ttc tac gtt cag gcc tac aaa    1728
Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys 
                565                 570                 575     

tct ctg aga cac agg tca gcc aac atg gac gtg ctc atc gtc ctg gcc    1776
Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala 
            580                 585                 590         

aca agc att gct tat gtt tat tct ctg gtc atc ctg gtg gtt gct gtg    1824
Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val 
        595                 600                 605             

gct gag aag gcg gag agg agc cct gtg aca ttc ttc gac acg ccc ccc    1872
Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro 
    610                 615                 620                 

atg ctc ttt gtg ttc att gcc ctg ggc cgg tgg ctg gaa cac ttg gca    1920
Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala 
625                 630                 635                 640 

aag agc aaa acc tca gaa gcc ctg gct aaa ctc atg tct ctc caa gcc    1968
Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala 
                645                 650                 655     

aca gaa gcc acc gtt gtg acc ctt ggt gag gac aat tta atc atc agg    2016
Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg 
            660                 665                 670         

gag gag caa gtc ccc atg gag ctg gtg cag cgg ggc gat atc gtc aag    2064
Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys 
        675                 680                 685             

gtg gtc cct ggg gga aag ttt cca gtg gat ggg aaa gtc ctg gaa ggc    2112
Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly 
    690                 695                 700                 

aat acc atg gct gat gag tcc ctc atc aca gga gaa gcc atg cca gtc    2160
Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val 
705                 710                 715                 720 

act aag aaa ccc gga agc act gta att gcg ggg tct ata aat gca cat    2208
Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His 
                725                 730                 735     

ggc tct gtg ctc att aaa gct acc cac gtg ggc aat gac acc act ttg    2256
Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu 
            740                 745                 750         

gct cag att gtg aaa ctg gtg gaa gag gct cag atg tca aag gca ccc    2304
Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro 
        755                 760                 765             

att cag cag ctg gct gac cgg ttt agt gga tat ttt gtc cca ttt atc    2352
Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile 
    770                 775                 780                 

atc atc atg tca act ttg acg ttg gtg gta tgg att gta atc ggt ttt    2400
Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe 
785                 790                 795                 800 

atc gat ttt ggt gtt gtt cag aga tac ttt cct aac ccc aac aag cac    2448
Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His 
                805                 810                 815     

atc tcc cag aca gag gtg atc atc cgg ttt gct ttc cag acg tcc atc    2496
Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile 
            820                 825                 830         

acg gtg ctg tgc att gcc tgc ccc tgc tcc ctg ggg ctg gcc acg ccc    2544
Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro 
        835                 840                 845             

acg gct gtc atg gtg ggc acc ggg gtg gcc gcg cag aac ggc atc ctc    2592
Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu 
    850                 855                 860                 

atc aag gga ggc aag ccc ctg gag atg gcg cac aag ata aag act gtg    2640
Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val 
865                 870                 875                 880 

atg ttt gac aag act ggc acc att acc cat ggc gtc ccc agg gtc atg    2688
Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met 
                885                 890                 895     

cgg gtg ctc ctg ctg ggg gat gtg gcc aca ctg ccc ctc agg aag gtt    2736
Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val 
            900                 905                 910         

ctg gct gtg gtg ggg act gcg gag gcc agc agt gaa cac ccc ttg ggc    2784
Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly 
        915                 920                 925             

gtg gca gtc acc aaa tac tgt aaa gag gaa ctt gga aca gag acc ttg    2832
Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu 
    930                 935                 940                 

gga tac tgc acg gac ttc cag gca gtg cca ggc tgt gga att ggg tgc    2880
Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys 
945                 950                 955                 960 

aaa gtc agc aac gtg gaa ggc atc ctg gcc cac agt gag cgc cct ttg    2928
Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu 
                965                 970                 975     

agt gca ccg gcc agt cac ctg aat gag gct ggc agc ctt ccc gca gaa    2976
Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu 
            980                 985                 990         

aaa gat gca gtc ccc cag acc ttc tct gtg ctg att gga aac cgt gag    3024
Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu 
        995                 1000                1005            

tgg ctg agg cgc aac ggt tta acc att tct agc gat gtc agt gac gct    3072
Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala 
    1010                1015                1020                

atg aca gac cac gag atg aaa gga cag aca gcc atc ctg gtg gct att    3120
Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile 
1025                1030                1035                1040

gac ggt gtg ctc tgt ggg atg atc gca atc gca gac gct gtc aag cag    3168
Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln 
                1045                1050                1055    

gag gct gcc ctg gct gtg cac acg ctg cag agc atg ggt gtg gac gtg    3216
Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val 
            1060                1065                1070        

gtt ctg atc acg ggg gac aac cgg aag aca gcc aga gct att gcc acc    3264
Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr 
        1075                1080                1085            

cag gtt ggc atc aac aaa gtc ttt gca gag gtg ctg cct tcg cac aag    3312
Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys 
    1090                1095                1100                

gtg gcc aag gtc cag gag ctc cag aat aaa ggg aag aaa gtc gcc atg    3360
Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met 
1105                1110                1115                1120

gtg ggg gat ggg gtc aat gac tcc ccg gcc ttg gcc cag gca gac atg    3408
Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met 
                1125                1130                1135    

ggt gtg gcc att ggc acc ggc acg gat gtg gcc atc gag gca gcc gac    3456
Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp 
            1140                1145                1150        

gtc gtc ctt atc aga aat gat ttg ctg gat gtg gtg gct agc att cac    3504
Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His 
        1155                1160                1165            

ctt tcc aag agg act gtc cga agg ata cgc atc aac ctg gtc ctg gca    3552
Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala 
    1170                1175                1180                

ctg att tat aac ctg gtt ggg ata ccc att gca gca ggt gtc ttc atg    3600
Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met 
1185                1190                1195                1200

ccc atc ggc att gtg ctg cag ccc tgg atg ggc tca gcg gcc atg gca    3648
Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala 
                1205                1210                1215    

gcc tcc tct gtg tct gtg gtg ctc tca tcc ctg cag ctc aag tgc tat    3696
Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr 
            1220                1225                1230        

aag aag cct gac ctg gag agg tat gag gca cag gcg cat ggc cac atg    3744
Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met 
        1235                1240                1245            

aag ccc ctg acg gca tcc cag gtc agt gtg cac ata ggc atg gat gac    3792
Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp 
    1250                1255                1260                

agg tgg cgg gac tcc ccc agg gcc aca cca tgg gac cag gtc agc tat    3840
Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr 
1265                1270                1275                1280

gtc agc cag gtg tcg ctg tcc tcc ctg acg tcc gac aag cca tct cgg    3888
Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg 
                1285                1290                1295    

cac agc gct gca gca gac gat gat ggg gac aag tgg tct ctg ctc ctg    3936
His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu 
            1300                1305                1310        

aat ggc agg gat gag gag cag tac atc tga                            3966
Asn Gly Arg Asp Glu Glu Gln Tyr Ile     
        1315                1320        

<210> 13
<211> 1321
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..3966 from SEQ ID NO 12

<400> 13
Met Pro Glu Gln Glu Arg Gln Ile Thr Ala Arg Glu Gly Ala Ser Arg 
1               5                   10                  15      
Lys Ile Leu Ser Lys Leu Ser Leu Pro Thr Arg Ala Trp Glu Pro Ala 
            20                  25                  30          
Met Lys Lys Ser Phe Ala Phe Asp Asn Val Gly Tyr Glu Gly Gly Leu 
        35                  40                  45              
Asp Gly Leu Gly Pro Ser Ser Gln Val Ala Thr Ser Thr Val Arg Ile 
    50                  55                  60                  
Leu Gly Met Thr Cys Gln Ser Cys Val Lys Ser Ile Glu Asp Arg Ile 
65                  70                  75                  80  
Ser Asn Leu Lys Gly Ile Ile Ser Met Lys Val Ser Leu Glu Gln Gly 
                85                  90                  95      
Ser Ala Thr Val Lys Tyr Val Pro Ser Val Val Cys Leu Gln Gln Val 
            100                 105                 110         
Cys His Gln Ile Gly Asp Met Gly Phe Glu Ala Ser Ile Ala Glu Gly 
        115                 120                 125             
Lys Ala Ala Ser Trp Pro Ser Arg Ser Leu Pro Ala Gln Glu Ala Val 
    130                 135                 140                 
Val Lys Leu Arg Val Glu Gly Met Thr Cys Gln Ser Cys Val Ser Ser 
145                 150                 155                 160 
Ile Glu Gly Lys Val Arg Lys Leu Gln Gly Val Val Arg Val Lys Val 
                165                 170                 175     
Ser Leu Ser Asn Gln Glu Ala Val Ile Thr Tyr Gln Pro Tyr Leu Ile 
            180                 185                 190         
Gln Pro Glu Asp Leu Arg Asp His Val Asn Asp Met Gly Phe Glu Ala 
        195                 200                 205             
Ala Ile Lys Ser Lys Val Ala Pro Leu Ser Leu Gly Pro Ile Gly Met 
    210                 215                 220                 
Thr Cys Ala Ser Cys Val His Ser Ile Glu Gly Met Ile Ser Gln Leu 
225                 230                 235                 240 
Glu Gly Val Gln Gln Ile Ser Val Ser Leu Ala Glu Gly Thr Ala Thr 
                245                 250                 255     
Val Leu Tyr Asn Pro Ser Val Ile Ser Pro Glu Glu Leu Arg Ala Ala 
            260                 265                 270         
Ile Glu Asp Met Gly Phe Glu Ala Ser Val Val Ser Glu Ser Cys Ser 
        275                 280                 285             
Thr Asn Pro Leu Gly Asn His Ser Ala Gly Asn Ser Met Val Gln Thr 
    290                 295                 300                 
Thr Asp Gly Thr Pro Thr Ser Val Gln Glu Val Ala Pro His Thr Gly 
305                 310                 315                 320 
Arg Leu Pro Ala Asn His Ala Pro Asp Ile Leu Ala Lys Ser Pro Gln 
                325                 330                 335     
Ser Thr Arg Ala Val Ala Pro Gln Lys Cys Phe Leu Gln Ile Lys Gly 
            340                 345                 350         
Met Thr Cys Ala Ser Cys Val Ser Asn Ile Glu Arg Asn Leu Gln Lys 
        355                 360                 365             
Glu Ala Gly Val Leu Ser Val Leu Val Ala Leu Met Ala Gly Lys Ala 
    370                 375                 380                 
Glu Ile Lys Tyr Asp Pro Glu Val Ile Gln Pro Leu Glu Ile Ala Gln 
385                 390                 395                 400 
Phe Ile Gln Asp Leu Gly Phe Glu Ala Ala Val Met Glu Asp Tyr Ala 
                405                 410                 415     
Gly Ser Asp Gly Asn Ile Glu Leu Thr Ile Thr Gly Met Thr Cys Ala 
            420                 425                 430         
Ser Cys Val His Asn Ile Glu Ser Lys Leu Thr Arg Thr Asn Gly Ile 
        435                 440                 445             
Thr Tyr Ala Ser Val Ala Leu Ala Thr Ser Lys Ala Leu Val Lys Phe 
    450                 455                 460                 
Asp Pro Glu Ile Ile Gly Pro Arg Asp Ile Ile Lys Ile Ile Glu Glu 
465                 470                 475                 480 
Ile Gly Phe His Ala Ser Leu Ala Gln Arg Asn Pro Asn Ala His His 
                485                 490                 495     
Leu Asp His Lys Met Glu Ile Lys Gln Trp Lys Lys Ser Phe Leu Cys 
            500                 505                 510         
Ser Leu Val Phe Gly Ile Pro Val Met Ala Leu Met Ile Tyr Met Leu 
        515                 520                 525             
Ile Pro Ser Asn Glu Pro His Gln Ser Met Val Leu Asp His Asn Ile 
    530                 535                 540                 
Ile Pro Gly Leu Ser Ile Leu Asn Leu Ile Phe Phe Ile Leu Cys Thr 
545                 550                 555                 560 
Phe Val Gln Leu Leu Gly Gly Trp Tyr Phe Tyr Val Gln Ala Tyr Lys 
                565                 570                 575     
Ser Leu Arg His Arg Ser Ala Asn Met Asp Val Leu Ile Val Leu Ala 
            580                 585                 590         
Thr Ser Ile Ala Tyr Val Tyr Ser Leu Val Ile Leu Val Val Ala Val 
        595                 600                 605             
Ala Glu Lys Ala Glu Arg Ser Pro Val Thr Phe Phe Asp Thr Pro Pro 
    610                 615                 620                 
Met Leu Phe Val Phe Ile Ala Leu Gly Arg Trp Leu Glu His Leu Ala 
625                 630                 635                 640 
Lys Ser Lys Thr Ser Glu Ala Leu Ala Lys Leu Met Ser Leu Gln Ala 
                645                 650                 655     
Thr Glu Ala Thr Val Val Thr Leu Gly Glu Asp Asn Leu Ile Ile Arg 
            660                 665                 670         
Glu Glu Gln Val Pro Met Glu Leu Val Gln Arg Gly Asp Ile Val Lys 
        675                 680                 685             
Val Val Pro Gly Gly Lys Phe Pro Val Asp Gly Lys Val Leu Glu Gly 
    690                 695                 700                 
Asn Thr Met Ala Asp Glu Ser Leu Ile Thr Gly Glu Ala Met Pro Val 
705                 710                 715                 720 
Thr Lys Lys Pro Gly Ser Thr Val Ile Ala Gly Ser Ile Asn Ala His 
                725                 730                 735     
Gly Ser Val Leu Ile Lys Ala Thr His Val Gly Asn Asp Thr Thr Leu 
            740                 745                 750         
Ala Gln Ile Val Lys Leu Val Glu Glu Ala Gln Met Ser Lys Ala Pro 
        755                 760                 765             
Ile Gln Gln Leu Ala Asp Arg Phe Ser Gly Tyr Phe Val Pro Phe Ile 
    770                 775                 780                 
Ile Ile Met Ser Thr Leu Thr Leu Val Val Trp Ile Val Ile Gly Phe 
785                 790                 795                 800 
Ile Asp Phe Gly Val Val Gln Arg Tyr Phe Pro Asn Pro Asn Lys His 
                805                 810                 815     
Ile Ser Gln Thr Glu Val Ile Ile Arg Phe Ala Phe Gln Thr Ser Ile 
            820                 825                 830         
Thr Val Leu Cys Ile Ala Cys Pro Cys Ser Leu Gly Leu Ala Thr Pro 
        835                 840                 845             
Thr Ala Val Met Val Gly Thr Gly Val Ala Ala Gln Asn Gly Ile Leu 
    850                 855                 860                 
Ile Lys Gly Gly Lys Pro Leu Glu Met Ala His Lys Ile Lys Thr Val 
865                 870                 875                 880 
Met Phe Asp Lys Thr Gly Thr Ile Thr His Gly Val Pro Arg Val Met 
                885                 890                 895     
Arg Val Leu Leu Leu Gly Asp Val Ala Thr Leu Pro Leu Arg Lys Val 
            900                 905                 910         
Leu Ala Val Val Gly Thr Ala Glu Ala Ser Ser Glu His Pro Leu Gly 
        915                 920                 925             
Val Ala Val Thr Lys Tyr Cys Lys Glu Glu Leu Gly Thr Glu Thr Leu 
    930                 935                 940                 
Gly Tyr Cys Thr Asp Phe Gln Ala Val Pro Gly Cys Gly Ile Gly Cys 
945                 950                 955                 960 
Lys Val Ser Asn Val Glu Gly Ile Leu Ala His Ser Glu Arg Pro Leu 
                965                 970                 975     
Ser Ala Pro Ala Ser His Leu Asn Glu Ala Gly Ser Leu Pro Ala Glu 
            980                 985                 990         
Lys Asp Ala Val Pro Gln Thr Phe Ser Val Leu Ile Gly Asn Arg Glu 
        995                 1000                1005            
Trp Leu Arg Arg Asn Gly Leu Thr Ile Ser Ser Asp Val Ser Asp Ala 
    1010                1015                1020                
Met Thr Asp His Glu Met Lys Gly Gln Thr Ala Ile Leu Val Ala Ile 
1025                1030                1035                1040
Asp Gly Val Leu Cys Gly Met Ile Ala Ile Ala Asp Ala Val Lys Gln 
                1045                1050                1055    
Glu Ala Ala Leu Ala Val His Thr Leu Gln Ser Met Gly Val Asp Val 
            1060                1065                1070        
Val Leu Ile Thr Gly Asp Asn Arg Lys Thr Ala Arg Ala Ile Ala Thr 
        1075                1080                1085            
Gln Val Gly Ile Asn Lys Val Phe Ala Glu Val Leu Pro Ser His Lys 
    1090                1095                1100                
Val Ala Lys Val Gln Glu Leu Gln Asn Lys Gly Lys Lys Val Ala Met 
1105                1110                1115                1120
Val Gly Asp Gly Val Asn Asp Ser Pro Ala Leu Ala Gln Ala Asp Met 
                1125                1130                1135    
Gly Val Ala Ile Gly Thr Gly Thr Asp Val Ala Ile Glu Ala Ala Asp 
            1140                1145                1150        
Val Val Leu Ile Arg Asn Asp Leu Leu Asp Val Val Ala Ser Ile His 
        1155                1160                1165            
Leu Ser Lys Arg Thr Val Arg Arg Ile Arg Ile Asn Leu Val Leu Ala 
    1170                1175                1180                
Leu Ile Tyr Asn Leu Val Gly Ile Pro Ile Ala Ala Gly Val Phe Met 
1185                1190                1195                1200
Pro Ile Gly Ile Val Leu Gln Pro Trp Met Gly Ser Ala Ala Met Ala 
                1205                1210                1215    
Ala Ser Ser Val Ser Val Val Leu Ser Ser Leu Gln Leu Lys Cys Tyr 
            1220                1225                1230        
Lys Lys Pro Asp Leu Glu Arg Tyr Glu Ala Gln Ala His Gly His Met 
        1235                1240                1245            
Lys Pro Leu Thr Ala Ser Gln Val Ser Val His Ile Gly Met Asp Asp 
    1250                1255                1260                
Arg Trp Arg Asp Ser Pro Arg Ala Thr Pro Trp Asp Gln Val Ser Tyr 
1265                1270                1275                1280
Val Ser Gln Val Ser Leu Ser Ser Leu Thr Ser Asp Lys Pro Ser Arg 
                1285                1290                1295    
His Ser Ala Ala Ala Asp Asp Asp Gly Asp Lys Trp Ser Leu Leu Leu 
            1300                1305                1310        
Asn Gly Arg Asp Glu Glu Gln Tyr Ile 
        1315                1320    

<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer F1

<400> 14
ctagatgcgg ccgccaccat gcctg                                          25


<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer R1

<400> 15
ctgagaagaa gggcccaggc c                                              21


<210> 16
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer F2

<400> 16
ggcccttctt ctcagccgca gaagtgcttc ttacag                               36


<210> 17
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer R2

<400> 17
accaaaatcg ataaaaccga ttacaatcc                                      29


<210> 18
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer F3

<400> 18
acgcgtgcgg ccgccaccat gccag                                          25


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer R3

<400> 19
ctgggagcta ggtcccagtc c                                              21


<210> 20
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer F4

<400> 20
ggacctagct cccagcctca gaagtgtttt ctgcag                               36


<210> 21
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer R4

<400> 21
tgttcctcgc gaatgatcag gttgtcctc                                      29


