                         SEQUENCE LISTING

<110>  Winthrop-University Hospital
       Akirav, Eitan M.
 
<120>  DETECTION ASSAY TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED
        DNA

<130>  P/2526-181

<150>  62/090,611
<151>  2014-12-11

<150>  62/090,618
<151>  2014-12-11

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR Primer for insulin gene

<400>  1
gtgcggttta tatttggtgg aagtt                                             25


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer for insulin gene

<400>  2
acaacaataa acaattaact caccctacaa                                        30


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of methylated non-beta cell insulin DNA

<400>  3
acctcccgac gaatct                                                       16


<210>  4
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of methylated non-beta cell insulin DNA

<400>  4
tacctctcgt cgaatct                                                      17


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of demethylated beta cell insulin DNA

<400>  5
acctcccaac aaatct                                                       16


<210>  6
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for the detection of demethylated beta cell insulin dna

<400>  6
tacctcccat caaatct                                                      17


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer Forward PCR primer for nested PCR according to Akirav et 
       al. (2011)

<400>  7
ttaggggttt taaggtaggg tatttggt                                          28


<210>  8
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer Reverse PCR primer for nested PCR according to Akirav et 
       al. (2011)

<400>  8
accaaaaaca acaataaaca attaactcac cctacaa                                37


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Methylated Forward PCR primer for nested PCR according to 
       Akirav et al. (2011)

<400>  9
gtggatgcgt tttttgtttt tgttggc                                           27


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Methylated Reverse PCR primer for nested PCR according to 
       Akirav et al. (2011)

<400>  10
caccctacaa atcctctacc tcccg                                             25


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Demethylated Forward PCR primer for nested PCR according to
       Akirav et al. (2011)

<400>  11
ttgtggatgt gttttttgtt tttgttggt                                         29


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Demethylated Reverse PCR primer for nested PCR according to
       Akirav et al. (2011)

<400>  12
caccctacaa atcctctacc tccca                                             25


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypermethylated strand forward primer

<400>  13
gggtagttta gagtgatagg attaagatat                                        30


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypermethylated strand reverse primer

<400>  14
taaaaataaa ccaccctaaa aaaaa                                             25


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypermethylated strand probe

<400>  15
aacgttcttc ccaaaaaata tacga                                             25


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypomethylated strand forward primer

<400>  16
gggtagttta gagtgatagg attaagatat                                        30


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypomethylated strand reverse primer

<400>  17
taaaaataaa ccaccctaaa aaaaa                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 1, hypomethylated strand probe

<400>  18
aacattcttc ccaaaaaata tacaa                                             25


<210>  19
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypermethylated strand forward primer

<400>  19
gggtagttta gagtgatagg attaagatat                                        30


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypermethylated strand reverse primer

<400>  20
taaaaataaa ccaccctaaa aaaaa                                             25


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypermethylated strand probe

<400>  21
tacgacataa caattccact tcatccccga                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypomethylated strand forward primer

<400>  22
gggtagttta gagtgatagg attaagatat                                        30


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypomethylated strand reverse primer

<400>  23
taaaaataaa ccaccctaaa aaaaa                                             25


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human MOG Probe set 2, hypomethylated strand probe

<400>  24
tacaacataa caattccact tcatccccaa                                        30


<210>  25
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypermethylated strand forward primer

<400>  25
gagtgatagg attagggtat tttatt                                            26


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypermethylated strand reverse primer

<400>  26
tctacatctt aatccttacc atttc                                             25


<210>  27
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypermethylated strand probe

<400>  27
acccgtaaca tttttcccaa aaaaaatacg a                                      31


<210>  28
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypomethylated strand forward primer

<400>  28
gagtgatagg attagggtat tttatt                                            26


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypomethylated strand reverse primer

<400>  29
tctacatctt aatccttacc atttc                                             25


<210>  30
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 1, hypomethylated strand probe

<400>  30
acccataaca tttttcccaa aaaaaataca a                                      31


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypermethylated strand forward primer

<400>  31
gagtgatagg attagggtat tttatt                                            26


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypermethylated strand reverse primer

<400>  32
tctacatctt aatccttacc atttc                                             25


<210>  33
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypermethylated strand probe

<400>  33
tacgacacga caactctact tcat                                              24


<210>  34
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypomethylated strand forward primer

<400>  34
gagtgatagg attagggtat tttatt                                            26


<210>  35
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypomethylated strand reverse primer

<400>  35
tctacatctt aatccttacc atttc                                             25


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse MOG Probe set 2, hypomethylated strand probe

<400>  36
tacaacacaa caactctact tcat                                              24


<210>  37
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Total human Insulin probe (Tins_HEX) 5'-HEX-[037]-ZEN-[038]- 
       3IABkFQ

<400>  37
cttaaatat                                                                9


<210>  38
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Total human Insulin probe (Tins_HEX) 5'-HEX-[037]-ZEN-[038]- 
       3IABkFQ

<400>  38
ataaaaaaaa cctc                                                         14


<210>  39
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Demethylated human Insulin probe (HYPOhINS) 
       5'-6-FAM-[039]-ZEN-[040]- 3IABkFQ

<400>  39
acctcccaa                                                                9


<210>  40
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Demethylated human Insulin probe (HYPOhINS) 
       5'-6-FAM-[039]-ZEN-[040]- 3IABkFQ

<400>  40
caaatct                                                                  7


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Insulin forward PCR primer hIns_meth_F

<400>  41
gtgcggttta tatttggtgg aagtt                                             25


<210>  42
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human insulin PCR reverse primer hIns_meth_R

<400>  42
acaacaataa acaattaact caccctacaa                                        30


