                         SEQUENCE LISTING

<110>  ElitechGroup B.V.
       Afonina, Irina
       Belousov, Yevgeniy
 
<120>  Methods and Compositions for Detecting Antibiotic Resistant 
       Bacteria

<130>  ELI-0133

<150>  62/091007
<151>  2014-12-12

<160>  83    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-FAM-1 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6 amino 1H pyrazolo[3,4 d]pyrimidin 4(5H) one attached to 
       FAM

<220>
<221>  m
<222>  (16)..(16)
<223>  m is Eclipse Dark Quencher attached to minor groove binder

<400>  1
mcaggttccg gttttm                                                       16


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-L1 primer

<400>  2
aataaatcat gtcatttgcc gtgccatac                                         29


<210>  3
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-E1 primer

<400>  3
aataaatcat aagcagactg ggcagtcgg                                         29


<210>  4
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-4 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6 amino 1H pyrazolo[3,4 d]pyrimidin 4(5H) one attached to 
       AP593 dye

<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (18)..(18)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  4
mgmcacactc cagataam                                                     18


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-5 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6 amino 1H pyrazolo[3,4 d]pyrimidin 4(5H) one attached to 
       AP593 dye

<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (18)..(18)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  5
mgmcacactc aagataam                                                     18


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-15 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6 amino 1H pyrazolo[3,4 d]pyrimidin 4(5H) one attached to 
       AP593 dye

<220>
<221>  m
<222>  (5)..(6)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (10)..(10)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (12)..(12)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (19)..(19)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  6
mctgmmttam cmaatgagm                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-16 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (5)..(6)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (10)..(10)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (12)..(12)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (19)..(19)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  7
mctgmmttam cmaatgaam                                                    19


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L1 primer


<220>
<221>  m
<222>  (16)..(16)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<400>  8
aataaatcat ggaatmgagt ggcttaattc tc                                     32


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L2 primer


<220>
<221>  m
<222>  (16)..(16)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<400>  9
aataaatcat ggaatmgggt ggcttaattc tc                                     32


<210>  10
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L3 primer


<220>
<221>  m
<222>  (16)..(16)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<400>  10
aataaatcat ggaatmgaat ggcttaactc tc                                     32


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E7 primer

<400>  11
aataaatagg caaccaaacc actacgttat ct                                     32


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E8 primer

<400>  12
aataaatagg cagccaaact actaggttat ct                                     32


<210>  13
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-AP593-7 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (14)..(14)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  13
mtgcgcttcg gtcm                                                         14


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-L4 primer

<400>  14
aataacgcat tctctagaag gactctcatc                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-L6 primer

<400>  15
aataacgcac tctctaaaag cgctctcctc                                        30


<210>  16
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-E6 primer


<220>
<221>  m
<222>  (25)..(25)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  16
aataaatcac gaatgcgcag caccmggata ga                                     32


<210>  17
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-AP593-2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (15)..(15)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  17
macatgccgg gtttm                                                        15


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-L2 primer

<400>  18
aataaatcat aagtctggca gcacacttcc ta                                     32


<210>  19
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-E2 primer

<400>  19
aataaatcat aacgccatcc ctgacgatca aac                                    33


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-AP662-7 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye

<220>
<221>  m
<222>  (18)..(18)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  20
mtgtttttgg tggcatcm                                                     18


<210>  21
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-L1 primer

<400>  21
aataaatcaa tgcgtgtatt agccttatcg gc                                     32


<210>  22
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-E1 primer

<400>  22
aataaatcat tcttgccatt cctttgctac cg                                     32


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E6132-L primer

<400>  23
ctgcacggac cagttacttt acg                                               23


<210>  24
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E6132-E primer

<400>  24
ctcatttttt ctaccggaga tcttgt                                            26


<210>  25
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E6132-AP525-TM3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP525 dye

<220>
<221>  m
<222>  (17)..(17)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  25
maccacgtac cgcattm                                                      17


<210>  26
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-Ozr705-1 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       Quasar 705 dye

<220>
<221>  m
<222>  (16)..(16)
<223>  m is C attached to 
       (E)-4-((4-((2-chloro-4-nitrophenyl)diazenyl)-2,5-dimethoxyphenyl)
       (ethyl)amino)butanoic acid dye attached to minor groove binder

<400>  26
mgtggcatcg attatm                                                       16


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-AP662-7 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP622 dye attached to minor groove binder

<220>
<221>  m
<222>  (18)..(18)
<223>  m is G attached to Eclipse Dark Quencher

<400>  27
mtgtttttgg tggcatcm                                                     18


<210>  28
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flap sequence of primer

<400>  28
aataaatcat aa                                                           12


<210>  29
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flap sequence of primer

<400>  29
aataaatcat                                                              10


<210>  30
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flap sequence of primer

<400>  30
aataaatca                                                                9


<210>  31
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flap sequence of primer

<400>  31
aataaat                                                                  7


<210>  32
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flap sequence of primer

<400>  32
aataa                                                                    5


<210>  33
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence for internal control

<400>  33
ctgcacggac cagttacttt acggaccacg taccgcattg gtacaagatc tccggtagaa       60

aaaatgag                                                                68


<210>  34
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-E2 primer

<400>  34
aataaatcat aaacagcgag gccgtcatc                                         29


<210>  35
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-L3 primer

<400>  35
aataaatcat aacccaatcc ctcgagcg                                          28


<210>  36
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-L4 primer

<400>  36
aataaatcat aacccaatcc ctcgagcgc                                         29


<210>  37
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-F2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       FAM

<220>
<221>  m
<222>  (15)..(15)
<223>  m is G attached to Eclipse Dark Quencher and minor groove binder

<400>  37
mtctagccgc agcgm                                                        15


<210>  38
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-F3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       FAM

<220>
<221>  m
<222>  (15)..(15)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  38
mtctagccgc agcgm                                                        15


<210>  39
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-L5 primer

<400>  39
aataaatcat aacgggctga cggccttcat g                                      31


<210>  40
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-E3 primer

<400>  40
aataaatcat aaagctccca gcggtccaga                                        30


<210>  41
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-F4 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       FAM

<220>
<221>  m
<222>  (16)..(16)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  41
mcgataccac gttccm                                                       16


<210>  42
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-F5 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       FAM

<220>
<221>  m
<222>  (17)..(17)
<223>  m is A attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  42
mtggtatcgc cgatagm                                                      17


<210>  43
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KPC-F6 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       FAM attached to minor groove binder

<220>
<221>  m
<222>  (17)..(17)
<223>  m is G attached to Eclipse Dark Quencher

<400>  43
mgcgatacca cgttccm                                                      17


<210>  44
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L5 primer


<220>
<221>  m
<222>  (15)..(15)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (27)..(27)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  44
gtttatgttc atacmtcgtt tgaagamgtt aa                                     32


<210>  45
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L6 primer


<220>
<221>  m
<222>  (15)..(15)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (27)..(27)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  45
gtttatgttc atacmtcgtt cgaagamgtt aa                                     32


<210>  46
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E5 primer


<220>
<221>  m
<222>  (22)..(22)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (25)..(25)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  46
aataaataca agaaccacca amccmtgttt ag                                     32


<210>  47
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E6 primer


<220>
<221>  m
<222>  (22)..(22)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (25)..(25)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  47
aataaataca agaaccacta amccmtgttt ag                                     32


<210>  48
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (2)..(3)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (8)..(8)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (14)..(14)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (16)..(16)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  48
mmmcaacmcc ccamcm                                                       16


<210>  49
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-7 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye attached to minor groove binder

<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (7)..(7)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (11)..(11)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (14)..(14)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (15)..(15)
<223>  m is T attached to Eclipse Dark Quencher

<400>  49
mgmcacmctc magmm                                                        15


<210>  50
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L7 primer


<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (6)..(6)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (9)..(9)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (27)..(27)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  50
aamaamaama ttgaagtttt ttatccmggc cc                                     32


<210>  51
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-L8 primer


<220>
<221>  m
<222>  (15)..(15)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (18)..(18)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (21)..(21)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (26)..(26)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (28)..(28)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  51
tattggctag ttaamaamaa mattgmamtt tt                                     32


<210>  52
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E7 primer

<400>  52
aataaatagg caaccaaacc actacgttat ct                                     32


<210>  53
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-E8 primer

<400>  53
aataaatagg cagccaaact actaggttat ct                                     32


<210>  54
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (5)..(5)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (7)..(7)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (11)..(11)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (15)..(15)
<223>  m is T attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  54
mgmcmcmctc magam                                                        15


<210>  55
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IMP-AP593-7


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye attached to minor groove binder

<220>
<221>  m
<222>  (3)..(3)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (7)..(7)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (11)..(11)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (14)..(14)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (15)..(15)
<223>  m is T attached to minor groove binder

<400>  55
mgmcacmctc magmm                                                        15


<210>  56
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-L3 primer


<220>
<221>  m
<222>  (16)..(16)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<400>  56
aataaatcat aaggcmcttc tcgcggagat tg                                     32


<210>  57
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-E2 primer

<400>  57
aataaatcat aagacgcggt cgtcatgaaa g                                      31


<210>  58
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-E3 primer


<220>
<221>  m
<222>  (19)..(19)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (28)..(28)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  58
aataaatcat aagacgcgmt cgtcatgmaa g                                      31


<210>  59
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-E4 primer


<220>
<221>  m
<222>  (24)..(24)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  59
aataaatcat acgtggagac tgcmcgcgtt ac                                     32


<210>  60
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-AP593-2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (18)..(18)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  60
maagcaaatt ggacttcm                                                     18


<210>  61
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-AP593-4 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye attached to minor groove binder

<220>
<221>  m
<222>  (18)..(18)
<223>  m is C attached to Eclipse Dark Quencher

<400>  61
maagcaaatt ggmcttcm                                                     18


<210>  62
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-L4 primer

<400>  62
aataacgcat tctctagaag gactctcatc                                        30


<210>  63
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-E5 primer


<220>
<221>  m
<222>  (23)..(23)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  63
aataaatcat atgcgcagca ccmggataga ag                                     32


<210>  64
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-AP593-3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (17)..(17)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  64
mcgcttcggt ccagtam                                                      17


<210>  65
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VIM-AP593-6 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye attached to minor groove binder

<220>
<221>  m
<222>  (16)..(16)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (17)..(17)
<223>  m is G attached to Eclipse Dark Quencher

<400>  65
mcgcttcggt ccagtmm                                                      17


<210>  66
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-L2 primer

<400>  66
aataaatcat aagtctggca gcacacttcc ta                                     32


<210>  67
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-E2 primer

<400>  67
aataaatcat aacgccatcc ctgacgatca aac                                    33


<210>  68
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-AP593-2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (15)..(15)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  68
macatgccgg gtttm                                                        15


<210>  69
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-L3 primer


<220>
<221>  m
<222>  (26)..(26)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  69
aataaatcat aataccgcct ggaccmatga c                                      31


<210>  70
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-E3 primer

<400>  70
aataaatcat aaaccggcag gttgatctcc t                                      31


<210>  71
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NDM-AP593-3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP593 dye

<220>
<221>  m
<222>  (17)..(17)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  71
mcccagatcc tcaactm                                                      17


<210>  72
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-E2 primer

<400>  72
aataaatcat aaaaacgggc gaaccaagca                                        30


<210>  73
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-E3 primer

<400>  73
aataaatcat aacgcgtctg tccatcccac tt                                     32


<210>  74
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-AP662-2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye

<220>
<221>  m
<222>  (12)..(15)
<223>  m is 5-(4-hydroxy-but-1-ynyl)-1H-pyrimidine-2,4-dione

<220>
<221>  m
<222>  (19)..(19)
<223>  m is G attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  74
mctattggga ammmmaaam                                                    19


<210>  75
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-L3 primer

<400>  75
aataaatcat accgaagcca atggtgacta ta                                     32


<210>  76
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-E3 primer


<220>
<221>  m
<222>  (13)..(13)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<400>  76
aataaatcaa ccmacccacc agccaatctt ag                                     32


<210>  77
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-AP662-3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye

<220>
<221>  m
<222>  (10)..(10)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (18)..(18)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  77
mgctaaaacm ggatactm                                                     18


<210>  78
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-AP662-6 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye attached to minor groove binder

<220>
<221>  m
<222>  (9)..(9)
<223>  m is 3-aminobutynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one

<220>
<221>  m
<222>  (14)..(14)
<223>  m is 
       4-(4,6-Diamino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-but-3-yn-1-ol

<220>
<221>  m
<222>  (18)..(18)
<223>  m is G attached to Eclipse Dark Quencher

<400>  78
mctaaaacmg gatmctcm                                                     18


<210>  79
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-PBI1 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye and minor groove binder

<220>
<221>  m
<222>  (19)..(19)
<223>  m is A attached to Eclipse Dark Quencher

<400>  79
mataatcgat gccaccaam                                                    19


<210>  80
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-PBI2 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP662 dye attached to minor groove binder

<220>
<221>  m
<222>  (19)..(19)
<223>  m is A attached to Eclipse Dark Quencher

<400>  80
mataatcgat gccaccaam                                                    19


<210>  81
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-PBI3 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       AP680 dye attached to minor groove binder

<220>
<221>  m
<222>  (19)..(19)
<223>  m is A attached to Eclipse Dark Quencher

<400>  81
mataatcgat gccaccaam                                                    19


<210>  82
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-PBI4 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attahced to 
       AP680 dye attached to minor groove binder

<220>
<221>  m
<222>  (19)..(19)
<223>  m is A attached to Eclipse Dark Quencher

<400>  82
mataatcgat gccaccaam                                                    19


<210>  83
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OXA-Cy55-1 probe


<220>
<221>  m
<222>  (1)..(1)
<223>  m is 6-amino-1H pyrazolo[3,4-d]pyrimidin-4(5H)-one attached to 
       Cy5.5 dye

<220>
<221>  m
<222>  (16)..(16)
<223>  m is C attached to Eclipse Dark Quencher attached to minor groove
       binder

<400>  83
mgtggcatcg attatm                                                       16


