SEQUENCE LISTING
 
<110> Fundao PIO XII
 
<120> PROTEINA RECOMBINANTE; PROCESSO DE CONSTRUCAO DA MESMA E O USO PARA O
TRATAMENTO DE DISTURBIOS RELACIONADOS COM A PROLIFERACAO CELULAR INDESEJADA.
<130> CONSTRUCAO DE PROTEINA RECOMBINANTE
<160> 8
<210> SEQ.ID.NO:1
<211> 197
<212> PRT
<213> PROTEINA sfTRAIL
<400>
Met	Gly	Try	Ile	Pro	Glu	Ala	Pro	Arg	Asp	Gly	Gln	Ala										
 
												
Try	Val	Arg	Lys	Asp	Gly	Glu	Try	Val	Leu	Leu	Ser	Thr											
 
											
Phe	Leu	Ala	Ser	Val	Arg	Glu	Arg	Gly	Pro	Gln 	Arg	Val												
 
											
Ala	Ala	His 	Ile	Thr	Gly	Thr	Arg	Gly	Arg	Ser	Asn	Thr												
 
											
Leu 	Ser	Ser 	Pro	Asn	Ser	Lys	Asn	Glu	Lys 	Ala	Leu	Gly												
 
												
Arg	Lys	Ile	Asn	Ser	Trp	Glu	Ser	Ser	Arg	Ser	Gly	His												
	
													
Ser	Phe	Leu	Ser	Ans	Leu	His	Leu	Arg	Asn	Gly	Glu	Leu												
		 
												
Val	Ile	His	Glu	Lys	Gly	Phe	Tyr	Tyr	Ile	Try	Ser	Gln									
 
												
Thr	Tyr	Phe	Arg	Phe	Gln	Glu	Glu	Ile	Lys	Glu	Asn	Thr												
	
												
Lys	Asn	Asp	Lys	Gln	Met	Val	Gln	Tyr	Ile	Tyr	Lys	Thr												
	
												
Ser	Tyr	Pro	Asp	Pro	Ile	Leu	Leu	Met	Lys	Ser	Ala	Arg												
 
									
Asn	Ser	Cys	Trp	Ser	Lys	Asp	Ala	Glu	Tyr	Gly	Leu	Tyr												
 
											
Ser	Ile	Tyr	Gln	Gly	Gly	Ile	Phe	Glu	Leu	Lys	Glu	Asn												
 
										
Asp	Arg	Ile	Phe	Val	Ser	Val	Thr	Asn	Glu	His	Leu	Ile												
 
											
Asp	Met	Asp	His	Glu	Ala	Ser	Phe	Phe	Gly	Ala	Phe	Gly
												
												
Val	Gly
	
		
 
<210> SEQ.ID.NO: 2
<211> 42
<212> DNA
<213> PRIMER
<400>
Tccagaattc gtgatgctag cgtgagagaa agaggtcctc ag 42
 
<210> SEQ.ID.NO:3
<211> 42
<DNA> DNA
<213> PRIMER
<400>
 
Cgacgaattc agatgcggcc gcttagccaa ctaaaaaggc cc 42
 
<210> SEQ.ID.NO:4
<211> 550
<212> DNA
<213> ARTIFICIAL SEQUENCE
<400>
tccagaattc gtgatgctag cgtgagagaa agaggtcctc agagagtagc agctcacata 60
actgggacca gaggaagaag caacacattg tcttctccaa actccaagaa tgaaaaggct 60
ctgggccgca aaataaactc ctgggaatca tcaaggagtg ggcattcatt cctgagcaac 60
ttgcacttga ggaatggtga actggtcatc catgaaaaag ggttttacta catctattcc 60
caaacatact ttcgatttca ggaggaaata aaagaaaaca caaagaacga caaacaaatg 60
gtccaatata tttacaaata cacaagttat cctgacccta tattgttgat gaaaagtgct 60
agaaatagtt gttggtctaa agatgcagaa tatggactct attccatcta tcaaggggga 60
atatttgagc ttaaggaaaa tgacagaatt tttgtttctg taacaaatga gcacttgata 60
gacatggacc atgaagccag ttttttcggg gcctttttag ttggctaagc ggccgcatct 60
gaattcgtcg 10
 
<210> SEQ.ID.NO 5
<211> 1608
<212> DNA
<213> ARTIFICIAL SEQUENCE
<400>
gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg 60
gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 60
atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 60
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 60
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 60
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 60
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 60
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 60
acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat 60
tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc gcagcaccat 60
ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc 60
agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 60
gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 60
cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 60
taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 60
gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 60
agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat tcttctgaca 60
caacagtctc gaacttaagg ctagagccac catgattgaa caagatggat tgcacgcagg 60
ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 60
ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 60
gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 60
ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 60
ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 60
cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 60
ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 60
cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgc 48
 
<210> SEQ.ID NO:6
<211> 27 
<212> PRT
<213> FOLDON
<400>
Met	Gly	Try	Ile	Pro	Glu	Ala	Pro	Arg	Asp	Gly	Gln	Ala
																								
 
Try	Val	Arg	Lys	Asp	Gly	Glu	Try	Val	Leu	Leu	Ser	Thr
																							
 
Phe
 
 
<210> SEQ.ID.NO:7
<211> 90
<212> DNA
<213> PRIMER
<400>
ctagcatggg ctacatcccc gaggccccca gagacggcca ggcctacgtg agaaaggacg 60
gcgagtgggt gctgctgagc accttcctgg 30
 
<210> SEQ.ID.NO:8
<211> 90
<212> DNA
<213> PRIMER
<400>
ctagccagga aggtgctcag cagcacccac tcgccgtcct ttctcacgta ggcctggccg 60
tctctggggg cctcggggat gtagcccatg 30
 
<210> SEQ. ID. NO: 9
<211> 94
<212> DNA
<213> ARTIFICIAL SEQUENCE
<400>
ctagcatggg ctacatcccc gaggccccca gagacggcca ggcctacgtg agaaaggacg 60
gcgagtgggt gctgctgagc accttcctgg gatc 34
 
<201> SEQ.ID.NO:10
<211> 6138
<212> DNA
<213> ARTIFICIAL SEQUENCE 
<400>
aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag 60
taaaacctct acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg 60
aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc 60
gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 60
acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 60
cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 60
tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 60
gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 60
cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 60
gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 60
gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat 60
ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct 60
ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt 60
gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 60
atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 60
tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 60
cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 60
tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 60
tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact 60
taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 60
ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 60
cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 60
tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 60
tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 60
cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 60
catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 60
ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 60
ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 60
ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 60
tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 60
ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 60
atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 60
cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 60
caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca 60
tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atccgcgtat ggtgcactct 60
cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 60
tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 60
ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 60
gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 60
gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 60
acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 60
aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 60
attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 60
tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 60
gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 60
cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 60
tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 60
agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 60
tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 60
tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 60
tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 60
acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 60
accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 60
tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 60
cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 60
tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 60
actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 60
tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 60
cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 60
gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 60
tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 60
gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 60
gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 60
ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 60
acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 60
agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 60
cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 60
tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 60
gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 60
ttttgctcac atggctcgac agatcttcaa tattggccat tagccatatt attcattggt 60
tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat 60
atgtacattt atattggctc atgtccaata tgaccgccat gttggcattg attattgact 60
agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 60
gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 60
acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 60
tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 60
agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 60
atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 60
atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga 60
tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 60
gactttccaa aatgtcgtaa caactgcgat cgcccgcccc gttgacgcaa atgggcggta 60
ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcacta 60
gaagctttat tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt gcttctgaca 60
caacagtctc gaacttaagc tgcagtgact ctcttaaggt agccttgcag aagttggtcg 60
tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 60
ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 60
gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 60
ggctagagta cttaatacga ctcactatag gctagcatgg gctacatccc cgaggccccc 60
agagacggcc aggcctacgt gagaaaggac ggcgagtggg tgctgctgag caccttcctg 60
gctagcgtga gagaaagagg tcctcagaga gtagcagctc acataaccgg gaccagagga 60
agaagcaaca cattgtcttc tccaaactcc aagaatgaaa aggctctggg ccgcaaaata 60
aactcctggg aatcatcaag gagtgggcat tcattcctga gcaacttgca cttgaggaat 60
ggtgaactgg tcatccatga aaaagggttt tactacatct attcccaaac atactttcga 60
tttcaggagg aaataaaaga aaacacaaag aacgacaaac aaatggtcca atatatttac 60
aaatacacaa gttatcctga ccctatattg ttgatgaaaa gtgctagaaa tagttgttgg 60
tctaaagatg cagaatatgg actctattcc atctatcaag ggggaatatt tgagcttaag 60
gaaaatgaca gaatttttgt ttctgtaaca aatgagcact tgatagacat ggaccatgaa 60
gccagttttt tcggggcctt tttagttggc taagcggccg caatcacgaa ttcacgcgtg 60
gtacctctag agtcgacccg ggcggccgct tccctttagt gagggttaat gcttcgagca 60
gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 60
tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 60
aaacaagtta acaacaac 18