                         SEQUENCE LISTING

<110>  Merial, Inc.
       Lawrence, Paulraj
       Bey, Russell
       Stoll, Mike
       Bumgardner, Eric
       Stine, Douglas
 
<120>  Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of 
       Anti-Mycoplasma hyorhinis IgG in Swine Serum

<130>  MER 14-250

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p37 Forward Primer

<400>  1
gggattgaag tggttgtctg atttaag                                           27


<210>  2
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p37 Reverse Primer

<400>  2
caatgaacca gatgcagaac aatc                                              24


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p37 Forward Primer

<400>  3
gacgacgaca agatgcaggc ctctgcagtc gac                                    33


<210>  4
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p37 Reverse Primer

<400>  4
gaggagaagc ccggttattt aatcgccttt tcgtag                                 36


<210>  5
<211>  460
<212>  PRT
<213>  Mycobacterium hyorhinis

<400>  5

Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Lys Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Met Lys Ala Ser Ala 
        35                  40                  45              


Val Asp Gly Pro Gly Ile Arg Ser Asn Met Leu Lys Lys Leu Lys Asn 
    50                  55                  60                  


Phe Ile Leu Phe Ser Ser Ile Phe Ser Pro Ile Ala Phe Ala Ile Ser 
65                  70                  75                  80  


Cys Ser Asn Thr Gly Val Val Lys Gln Glu Asp Val Ser Val Ser Gln 
                85                  90                  95      


Gly Gln Trp Asp Lys Ser Ile Thr Phe Gly Val Ser Glu Ala Trp Leu 
            100                 105                 110         


Asn Lys Lys Lys Gly Gly Glu Lys Val Asn Lys Glu Val Ile Asn Thr 
        115                 120                 125             


Phe Leu Glu Asn Phe Lys Lys Glu Phe Asn Lys Leu Lys Asn Ala Asn 
    130                 135                 140                 


Asp Lys Thr Lys Asn Phe Asp Asp Val Asp Phe Lys Val Thr Pro Ile 
145                 150                 155                 160 


Gln Asp Phe Thr Val Leu Leu Asn Asn Leu Ser Thr Asp Asn Pro Glu 
                165                 170                 175     


Leu Asp Phe Gly Ile Asn Ala Ser Gly Lys Leu Val Glu Phe Leu Lys 
            180                 185                 190         


Asn Asn Pro Gly Ile Ile Thr Pro Ala Leu Glu Thr Thr Thr Asn Ser 
        195                 200                 205             


Phe Val Phe Asp Lys Glu Lys Asp Lys Phe Tyr Val Asp Gly Thr Asp 
    210                 215                 220                 


Ser Asp Pro Leu Val Lys Ile Ala Lys Glu Ile Asn Lys Ile Phe Val 
225                 230                 235                 240 


Glu Thr Pro Tyr Ala Ser Trp Thr Asp Glu Asn His Lys Trp Asn Gly 
                245                 250                 255     


Asn Val Tyr Gln Ser Val Tyr Asp Pro Thr Val Gln Ala Asn Phe Tyr 
            260                 265                 270         


Arg Gly Met Ile Trp Ile Lys Gly Asn Asp Glu Thr Leu Ala Lys Ile 
        275                 280                 285             


Lys Lys Ala Trp Asn Asp Lys Asp Trp Asn Thr Phe Arg Asn Phe Gly 
    290                 295                 300                 


Ile Leu His Gly Lys Asp Asn Ser Phe Ser Lys Phe Lys Leu Glu Glu 
305                 310                 315                 320 


Thr Ile Leu Lys Asn His Phe Gln Asn Lys Phe Thr Thr Leu Asn Glu 
                325                 330                 335     


Asp Arg Ser Ala His Pro Asn Ala Tyr Lys Gln Lys Ser Ala Asp Thr 
            340                 345                 350         


Leu Gly Thr Leu Asp Asp Phe His Ile Ala Phe Ser Glu Glu Gly Ser 
        355                 360                 365             


Phe Ala Trp Thr His Asn Lys Ser Ala Thr Lys Pro Phe Glu Thr Lys 
    370                 375                 380                 


Ala Asn Glu Lys Met Glu Ala Leu Ile Val Thr Asn Pro Ile Pro Tyr 
385                 390                 395                 400 


Asp Val Gly Val Phe Arg Lys Ser Val Asn Gln Leu Glu Gln Asn Leu 
                405                 410                 415     


Ile Val Gln Thr Phe Ile Asn Leu Ala Lys Asn Lys Gln Asp Thr Tyr 
            420                 425                 430         


Gly Pro Leu Leu Gly Tyr Asn Gly Tyr Lys Lys Ile Asp Asn Phe Gln 
        435                 440                 445             


Lys Glu Ile Val Glu Val Tyr Glu Lys Ala Ile Lys 
    450                 455                 460 


<210>  6
<211>  403
<212>  PRT
<213>  Mycobacterium hyorhinis

<400>  6

Leu Leu Lys Lys Phe Lys Asn Phe Ile Leu Phe Ser Ser Ile Phe Ser 
1               5                   10                  15      


Pro Ile Ala Phe Ala Ile Ser Cys Ser Asn Thr Gly Val Val Lys Gln 
            20                  25                  30          


Glu Asp Val Ser Val Ser Gln Gly Gln Trp Asp Lys Ser Ile Thr Phe 
        35                  40                  45              


Gly Val Ser Glu Ala Trp Leu Asn Lys Lys Lys Gly Asp Lys Glu Val 
    50                  55                  60                  


Asn Lys Glu Val Ile Asn Thr Phe Leu Glu Asn Phe Lys Lys Glu Phe 
65                  70                  75                  80  


Asn Lys Leu Lys Asn Ala Asn Asp Lys Thr Lys Asn Phe Asp Asp Val 
                85                  90                  95      


Asp Phe Lys Val Thr Pro Ile Gln Asp Ser Thr Val Leu Leu Asn Asn 
            100                 105                 110         


Leu Ser Thr Asp Asn Pro Glu Leu Asp Phe Gly Ile Asn Ala Ser Gly 
        115                 120                 125             


Lys Leu Val Glu Phe Leu Lys Asn Asn Pro Gly Ile Ile Thr Pro Ala 
    130                 135                 140                 


Leu Glu Thr Thr Thr Asn Ser Phe Val Phe Asp Lys Glu Lys Asp Lys 
145                 150                 155                 160 


Phe Tyr Val Asp Gly Thr Asp Ser Asp Pro Leu Val Lys Ile Ala Lys 
                165                 170                 175     


Glu Ile Asn Lys Ile Phe Val Glu Thr Pro Tyr Ala Ser Trp Thr Asp 
            180                 185                 190         


Glu Asn His Lys Trp Asn Gly Asn Val Tyr Gln Ser Val Tyr Asp Pro 
        195                 200                 205             


Thr Val Gln Ala Asn Phe Tyr Arg Gly Met Ile Trp Ile Lys Gly Asn 
    210                 215                 220                 


Asp Glu Thr Leu Ala Lys Ile Lys Lys Ala Trp Asn Asp Lys Asp Trp 
225                 230                 235                 240 


Asn Thr Phe Arg Asn Phe Gly Ile Leu His Gly Lys Asp Asn Ser Ser 
                245                 250                 255     


Ser Lys Phe Lys Leu Glu Glu Thr Ile Leu Lys Asn His Phe Gln Asn 
            260                 265                 270         


Lys Phe Thr Thr Leu Asn Glu Asp Arg Ser Ala His Pro Asn Ala Tyr 
        275                 280                 285             


Lys Gln Lys Ser Ala Asp Thr Leu Gly Thr Leu Asp Asp Phe His Ile 
    290                 295                 300                 


Ala Phe Ser Glu Glu Gly Ser Phe Ala Trp Thr His Asn Lys Ser Ala 
305                 310                 315                 320 


Thr Lys Pro Phe Glu Thr Lys Ala Asn Glu Lys Met Glu Ala Leu Ile 
                325                 330                 335     


Val Thr Asn Pro Ile Pro Tyr Asp Val Gly Val Phe Arg Lys Ser Val 
            340                 345                 350         


Asn Gln Leu Glu Gln Asn Leu Ile Val Gln Thr Phe Ile Asn Leu Ala 
        355                 360                 365             


Lys Asn Lys Gln Asp Thr Tyr Gly Pro Leu Leu Gly Tyr Asn Gly Tyr 
    370                 375                 380                 


Lys Lys Ile Asp Asn Phe Gln Lys Glu Ile Val Glu Val Tyr Glu Lys 
385                 390                 395                 400 


Ala Ile Lys 
            


<210>  7
<211>  403
<212>  PRT
<213>  Mycobacterium hyorhinis

<400>  7

Leu Leu Lys Lys Phe Lys Asn Phe Ile Leu Phe Ser Ser Ile Phe Ser 
1               5                   10                  15      


Pro Ile Ala Phe Ala Ile Ser Cys Ser Asn Thr Gly Val Val Lys Gln 
            20                  25                  30          


Glu Asp Val Ser Val Ser Gln Gly Gln Trp Asp Lys Ser Ile Thr Phe 
        35                  40                  45              


Gly Val Ser Glu Ala Trp Leu Asn Lys Lys Lys Gly Asp Lys Glu Val 
    50                  55                  60                  


Asn Lys Glu Val Ile Asn Thr Phe Leu Glu Asn Phe Lys Lys Glu Phe 
65                  70                  75                  80  


Asn Lys Leu Lys Asn Ala Asn Asp Lys Thr Lys Asn Phe Asp Asp Val 
                85                  90                  95      


Asp Phe Lys Val Thr Pro Ile Gln Asp Ser Thr Val Leu Leu Asn Asn 
            100                 105                 110         


Leu Ser Thr Asp Asn Pro Glu Leu Asp Phe Gly Ile Asn Ala Ser Gly 
        115                 120                 125             


Lys Leu Val Glu Phe Leu Lys Asn Asn Pro Gly Ile Ile Thr Pro Ala 
    130                 135                 140                 


Leu Glu Thr Thr Thr Asn Ser Phe Val Phe Asp Lys Glu Lys Asp Lys 
145                 150                 155                 160 


Phe Tyr Val Asp Gly Lys Asp Ser Asp Pro Leu Val Lys Ile Ala Lys 
                165                 170                 175     


Glu Ile Asn Lys Ile Phe Val Glu Thr Pro Tyr Ala Ser Trp Thr Asp 
            180                 185                 190         


Glu Asn His Lys Trp Asn Gly Asn Val Tyr Gln Ser Val Tyr Asp Pro 
        195                 200                 205             


Thr Val Gln Ala Asn Phe Tyr Arg Gly Met Ile Trp Ile Lys Gly Asn 
    210                 215                 220                 


Asp Glu Thr Leu Ala Lys Ile Lys Lys Ala Trp Asn Asp Lys Asp Trp 
225                 230                 235                 240 


Asn Thr Phe Arg Asn Phe Gly Ile Leu His Gly Lys Asp Asn Ser Ser 
                245                 250                 255     


Ser Lys Phe Lys Leu Glu Glu Thr Ile Leu Lys Asn His Phe Gln Asn 
            260                 265                 270         


Lys Phe Thr Thr Leu Asn Glu Asp Arg Ser Ala His Pro Asn Ala Tyr 
        275                 280                 285             


Lys Gln Lys Ser Ala Asp Thr Leu Gly Thr Leu Asp Asp Phe His Ile 
    290                 295                 300                 


Ala Phe Ser Glu Glu Gly Ser Phe Ala Trp Thr His Asn Lys Ser Ala 
305                 310                 315                 320 


Thr Lys Pro Phe Glu Thr Lys Ala Asn Glu Lys Met Glu Ala Leu Ile 
                325                 330                 335     


Val Thr Asn Pro Ile Pro Tyr Asp Val Gly Val Phe Arg Lys Ser Val 
            340                 345                 350         


Asn Gln Leu Glu Gln Asn Leu Ile Val Gln Thr Phe Ile Asn Leu Ala 
        355                 360                 365             


Lys Asn Lys Gln Asp Thr Tyr Gly Pro Leu Leu Gly Tyr Asn Gly Tyr 
    370                 375                 380                 


Lys Lys Ile Asp Asn Phe Gln Lys Glu Ile Val Glu Val Tyr Glu Lys 
385                 390                 395                 400 


Ala Ile Lys 
            


