
                                SEQUENCE LISTING

<110> Juno Therapeutics, Inc.

<120> METHODS AND COMPOSITIONS FOR ADOPTIVE CELL THERAPY

<130> 735042001240

<140> Not Yet Assigned    
<141> Concurrently Herewith   

<150> US 62/087,224       
<151> 2014-12-03  

<160> 159

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> IgG4 hinge

<400> 1
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10          


<210> 2
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> CD28 transmembrane domain

<400> 2
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25          


<210> 3
<211> 42
<212> PRT
<213> Homo sapiens

<220> 
<223> 4-1BB costimulatory domain

<400> 3
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 4
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> CD3-zeta intracellular signaling domain

<400> 4
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 5
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> CD28-4-1BB

<400> 5
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 6
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 6
Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys
 1               5                  10                  15      
Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe
            20                  25                  30  


<210> 7
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 7
Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10                  15      


<210> 8
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 8
Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10                  15  


<210> 9
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 9
Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys
 1               5                  10                  15  


<210> 10
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 10
Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10                  


<210> 11
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 11
Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu
 1               5                  10              


<210> 12
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 12
Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10              


<210> 13
<211> 32
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> Variant junction region

<400> 13
Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Asn
 1               5                  10                  15      
Asn Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe
            20                  25                  30          


<210> 14
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> Variant peptide

<400> 14
Ile Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys Leu
 1               5                  10                  


<210> 15
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<220> 
<223> Variant peptide

<400> 15
Ile Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys
 1               5                  10              


<210> 16
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 16
Val Ala Phe Ile Ile Phe Trp Val Lys
 1               5                  


<210> 17
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 17
Ala Phe Ile Ile Phe Trp Val Lys Arg
 1               5                  


<210> 18
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 18
Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  


<210> 19
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 19
Ile Ile Phe Trp Val Lys Arg Gly Arg
 1               5                  


<210> 20
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 20
Ile Phe Trp Val Lys Arg Gly Arg Lys
 1               5                  


<210> 21
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 21
Phe Trp Val Lys Arg Gly Arg Lys Lys
 1               5                  


<210> 22
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic

<400> 22
Trp Val Lys Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 23
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      

<400> 23
Val Ala Phe Ile Ile Phe Trp Val Ser
 1               5                  


<210> 24
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      

<400> 24
Ala Phe Ile Ile Phe Trp Val Ser Arg
 1               5                  


<210> 25
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      

<400> 25
Phe Ile Ile Phe Trp Val Ser Arg Gly
 1               5                  


<210> 26
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      
<400> 26
Ile Ile Phe Trp Val Ser Arg Gly Arg
 1               5                  


<210> 27
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      
<400> 27
Ile Phe Trp Val Ser Arg Gly Arg Lys
 1               5                  


<210> 28
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      
<400> 28
Phe Trp Val Ser Arg Gly Arg Lys Lys
 1               5                  


<210> 29
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28S
      
<400> 29
Trp Val Ser Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 30
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L
        
<400> 30
Val Ala Phe Ile Ile Phe Trp Val Leu
 1               5                  


<210> 31
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L 
      
<400> 31
Ala Phe Ile Ile Phe Trp Val Leu Arg
 1               5                  


<210> 32
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L     
     
<400> 32
Phe Ile Ile Phe Trp Val Leu Arg Gly
 1               5                  


<210> 33
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L      
      
<400> 33
Ile Ile Phe Trp Val Leu Arg Gly Arg
 1               5                  


<210> 34
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L      
      
<400> 34
Ile Phe Trp Val Leu Arg Gly Arg Lys
 1               5                  


<210> 35
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L      
      
<400> 35
Phe Trp Val Leu Arg Gly Arg Lys Lys
 1               5                  


<210> 36
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28L      
      
<400> 36
Trp Val Leu Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 37
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
      
<400> 37
Val Ala Phe Ile Ile Phe Trp Val His
 1               5                  


<210> 38
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
      
<400> 38
Ala Phe Ile Ile Phe Trp Val His Arg
 1               5                  


<210> 39
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
     
<400> 39
Phe Ile Ile Phe Trp Val His Arg Gly
 1               5                  


<210> 40
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H    
      
<400> 40
Ile Ile Phe Trp Val His Arg Gly Arg
 1               5                  


<210> 41
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
      
<400> 41
Ile Phe Trp Val His Arg Gly Arg Lys
 1               5                  


<210> 42
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
      
<400> 42
Phe Trp Val His Arg Gly Arg Lys Lys
 1               5                  


<210> 43
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28H      
      
<400> 43
Trp Val His Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 44
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 44
Val Ala Phe Ile Ile Phe Trp Val Ala
 1               5                  


<210> 45
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 45
Ala Phe Ile Ile Phe Trp Val Ala Arg
 1               5                  


<210> 46
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 46
Ile Ile Phe Trp Val Ala Arg Gly Arg
 1               5                  


<210> 47
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 47
Ile Phe Trp Val Ala Arg Gly Arg Lys
 1               5                  


<210> 48
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 48
Phe Trp Val Ala Arg Gly Arg Lys Lys
 1               5                  


<210> 49
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 49
Trp Val Ala Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 50
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q     
      
<400> 50
Val Ala Phe Ile Ile Phe Trp Val Gln
 1               5                  


<210> 51
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q     
      
<400> 51
Ala Phe Ile Ile Phe Trp Val Gln Arg
 1               5                  


<210> 52
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q     
      
<400> 52
Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  


<210> 53
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q    
      
<400> 53
Ile Ile Phe Trp Val Gln Arg Gly Arg
 1               5                  


<210> 54
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q   
      
<400> 54
Ile Phe Trp Val Gln Arg Gly Arg Lys
 1               5                  


<210> 55
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q    
      
<400> 55
Phe Trp Val Gln Arg Gly Arg Lys Lys
 1               5                  


<210> 56
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q   
      
<400> 56
Trp Val Gln Arg Gly Arg Lys Lys Leu
 1               5                  


<210> 57
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31S   
      
<400> 57
Ile Ile Phe Trp Val Lys Arg Gly Ser
 1               5                  


<210> 58
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31S    
      
<400> 58
Ile Phe Trp Val Lys Arg Gly Ser Lys
 1               5                  


<210> 59
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31S   
      
<400> 59
Phe Trp Val Lys Arg Gly Ser Lys Lys
 1               5                  


<210> 60
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31S   
      
<400> 60
Trp Val Lys Arg Gly Ser Lys Lys Leu
 1               5                  


<210> 61
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31L     
      
<400> 61
Ile Ile Phe Trp Val Lys Arg Gly Leu
 1               5                  


<210> 62
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31L    
      
<400> 62
Ile Phe Trp Val Lys Arg Gly Leu Lys
 1               5                  


<210> 63
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31L   
      
<400> 63
Phe Trp Val Lys Arg Gly Leu Lys Lys
 1               5                  


<210> 64
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31L    
      
<400> 64
Trp Val Lys Arg Gly Leu Lys Lys Leu
 1               5                  


<210> 65
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31H   
      
<400> 65
Ile Ile Phe Trp Val Lys Arg Gly His
 1               5                  


<210> 66
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31H   
      
<400> 66
Ile Phe Trp Val Lys Arg Gly His Lys
 1               5                  


<210> 67
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31H    
      
<400> 67
Phe Trp Val Lys Arg Gly His Lys Lys
 1               5                  


<210> 68
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31H    
      
<400> 68
Trp Val Lys Arg Gly His Lys Lys Leu
 1               5                  


<210> 69
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31A     
      
<400> 69
Ile Ile Phe Trp Val Lys Arg Gly Ala
 1               5                  


<210> 70
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31A    
      
<400> 70
Ile Phe Trp Val Lys Arg Gly Ala Lys
 1               5                  


<210> 71
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31A     
      
<400> 71
Phe Trp Val Lys Arg Gly Ala Lys Lys
 1               5                  


<210> 72
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31A     
      
<400> 72
Trp Val Lys Arg Gly Ala Lys Lys Leu
 1               5                  


<210> 73
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31N     
      
<400> 73
Ile Ile Phe Trp Val Lys Arg Gly Asn
 1               5                  


<210> 74
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31N     
      
<400> 74
Ile Phe Trp Val Lys Arg Gly Asn Lys
 1               5                  


<210> 75
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31N     
      
<400> 75
Phe Trp Val Lys Arg Gly Asn Lys Lys
 1               5                  


<210> 76
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide R31N    
      
<400> 76
Trp Val Lys Arg Gly Asn Lys Lys Leu
 1               5                  


<210> 77
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31S    
      
<400> 77
Ile Ile Phe Trp Val Gln Arg Gly Ser
 1               5                  


<210> 78
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31S      
      
<400> 78
Ile Phe Trp Val Gln Arg Gly Ser Lys
 1               5                  


<210> 79
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31S    
      
<400> 79
Phe Trp Val Gln Arg Gly Ser Lys Lys
 1               5                  


<210> 80
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31S    
      
<400> 80
Trp Val Gln Arg Gly Ser Lys Lys Leu
 1               5                  


<210> 81
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31A     
      
<400> 81
Ile Ile Phe Trp Val Gln Arg Gly Ala
 1               5                  


<210> 82
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31A     
      
<400> 82
Ile Phe Trp Val Gln Arg Gly Ala Lys
 1               5                  


<210> 83
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31A    
      
<400> 83
Phe Trp Val Gln Arg Gly Ala Lys Lys
 1               5                  


<210> 84
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31A     
      
<400> 84
Trp Val Gln Arg Gly Ala Lys Lys Leu
 1               5                  


<210> 85
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31N     
      
<400> 85
Ile Ile Phe Trp Val Gln Arg Gly Asn
 1               5                  


<210> 86
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31N     
      
<400> 86
Ile Phe Trp Val Gln Arg Gly Asn Lys
 1               5                  


<210> 87
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31N    
      
<400> 87
Phe Trp Val Gln Arg Gly Asn Lys Lys
 1               5                  


<210> 88
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/R31N     
      
<400> 88
Trp Val Gln Arg Gly Asn Lys Lys Leu
 1               5                  


<210> 89
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide L34S     
      
<400> 89
Trp Val Lys Arg Gly Arg Lys Lys Ser
 1               5                  


<210> 90
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide L34A     
      
<400> 90
Trp Val Lys Arg Gly Arg Lys Lys Ala
 1               5                  


<210> 91
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/L34S    
      
<400> 91
Trp Val Gln Arg Gly Asn Lys Lys Ser
 1               5                  


<210> 92
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28Q/L34A     
      
<400> 92
Trp Val Gln Arg Gly Asn Lys Lys Ala
 1               5                  


<210> 93
<211> 255
<212> PRT
<213> Homo sapiens     
      
<220> 
<223> 4-1BB

<400> 93
Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu
 1               5                  10                  15      
Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro
            20                  25                  30          
Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys
        35                  40                  45              
Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile
    50                  55                  60                  
Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser
65                  70                  75                  80  
Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly
                85                  90                  95      
Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu
            100                 105                 110         
Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln
        115                 120                 125             
Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys
    130                 135                 140                 
Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro
145                 150                 155                 160 
Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala
                165                 170                 175     
Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu
            180                 185                 190         
Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu
        195                 200                 205             
Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe
    210                 215                 220                 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly
225                 230                 235                 240 
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
                245                 250                 255 


<210> 94
<211> 255
<212> PRT
<213> Homo sapiens  
      
<220> 
<223> CD28

<400> 94
Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu
 1               5                  10                  15      
Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro
            20                  25                  30          
Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys
        35                  40                  45              
Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile
    50                  55                  60                  
Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser
65                  70                  75                  80  
Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly
                85                  90                  95      
Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu
            100                 105                 110         
Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln
        115                 120                 125             
Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys
    130                 135                 140                 
Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro
145                 150                 155                 160 
Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala
                165                 170                 175     
Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu
            180                 185                 190         
Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu
        195                 200                 205             
Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe
    210                 215                 220                 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly
225                 230                 235                 240 
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
                245                 250                 255 


<210> 95
<211> 164
<212> PRT
<213> Homo sapiens   
      
<220> 
<223> CD3 zeta chain

<400> 95
Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu
 1               5                  10                  15      
Pro Ile Thr Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys
            20                  25                  30          
Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu Thr Ala
        35                  40                  45              
Leu Phe Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr
    50                  55                  60                  
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg
65                  70                  75                  80  
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met
                85                  90                  95      
Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn
            100                 105                 110         
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met
        115                 120                 125             
Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly
    130                 135                 140                 
Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala
145                 150                 155                 160 
Leu Pro Pro Arg
                


<210> 96
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic     
      
<400> 96
Phe Ile Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys
 1               5                  10                  


<210> 97
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<400> 97
Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10                  


<210> 98
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<400> 98
Phe Ile Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys
 1               5                  10                  


<210> 99
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<400> 99
Ile Phe Trp Val Asn Asn Lys Arg Gly Arg Lys Lys Leu Leu
 1               5                  10                  


<210> 100
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28R    
      
<400> 100
Val Ala Phe Ile Ile Phe Trp Val Arg
 1               5                  


<210> 101
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28R    
      
<400> 101
Ala Phe Ile Ile Phe Trp Val Arg Arg
 1               5                  


<210> 102
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic peptide K28A    
      
<400> 102
Phe Ile Ile Phe Trp Val Ala Arg Gly
 1               5                  


<210> 103
<211> 28
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28 transmembrane domain    
      
<400> 103
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser
 1               5                  10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25              


<210> 104
<211> 66
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28    
      
<400> 104
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn
 1               5                  10                  15      
Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu
            20                  25                  30          
Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly
        35                  40                  45              
Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe
    50                  55                  60                  
Trp Val
65      


<210> 105
<211> 112
<212> PRT
<213> Homo sapiens

<220> 
<223> CD3 zeta

<400> 105
Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 106
<211> 36
<212> DNA
<213> Homo sapiens

<220> 
<223> spacer (IgG4hinge)

<400> 106
gaatctaagt acggaccgcc ctgcccccct tgccct                           36

<210> 107
<211> 119
<212> PRT
<213> Homo sapiens

<220> 
<223> Hinge-CH3 spacer

<400> 107
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg
 1               5                  10                  15      
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            20                  25                  30          
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        35                  40                  45              
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    50                  55                  60                  
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
65                  70                  75                  80  
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                85                  90                  95      
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            100                 105                 110         
Leu Ser Leu Ser Leu Gly Lys
        115                 


<210> 108
<211> 229
<212> PRT
<213> Homo sapiens

<220> 
<223> Hinge-CH2-CH3 spacer

<400> 108
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220                 
Leu Ser Leu Gly Lys
225                 


<210> 109
<211> 282
<212> PRT
<213> Homo sapiens

<220> 
<223> IgD-hinge-Fc

<400> 109
Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala
 1               5                  10                  15      
Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala
            20                  25                  30          
Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys
        35                  40                  45              
Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro
    50                  55                  60                  
Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln
65                  70                  75                  80  
Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly
                85                  90                  95      
Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val
            100                 105                 110         
Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly
        115                 120                 125             
Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn
    130                 135                 140                 
Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro
145                 150                 155                 160 
Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys
                165                 170                 175     
Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser
            180                 185                 190         
Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu
        195                 200                 205             
Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro
    210                 215                 220                 
Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser
225                 230                 235                 240 
Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr
                245                 250                 255     
Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg
            260                 265                 270         
Ser Leu Glu Val Ser Tyr Val Thr Asp His
        275                 280         


<210> 110
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> T2A

<400> 110
Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp
 1               5                  10                  15      
Val Glu Glu Asn Pro Gly Pro Arg
            20                  


<210> 111
<211> 357
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> tEGFR

<400> 111
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly
            20                  25                  30          
Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe
        35                  40                  45              
Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala
    50                  55                  60                  
Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu
65                  70                  75                  80  
Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile
                85                  90                  95      
Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu
            100                 105                 110         
Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala
        115                 120                 125             
Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu
    130                 135                 140                 
Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr
145                 150                 155                 160 
Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys
                165                 170                 175     
Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly
            180                 185                 190         
Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu
        195                 200                 205             
Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys
    210                 215                 220                 
Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu
225                 230                 235                 240 
Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met
                245                 250                 255     
Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala
            260                 265                 270         
His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val
        275                 280                 285             
Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His
    290                 295                 300                 
Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro
305                 310                 315                 320 
Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala
                325                 330                 335     
Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly
            340                 345                 350         
Ile Gly Leu Phe Met
        355         


<210> 112
<211> 41
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28 cytoplasmic domain

<400> 112
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 113
<211> 41
<212> PRT
<213> Homo sapiens

<220> 
<223> CD28 cytoplasmic domain

<400> 113
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 114
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28A    
      
<400> 114
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Ala Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 115
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28H    
      
<400> 115
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val His Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 116
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28L     
      
<400> 116
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Leu Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 117
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q    
      
<400> 117
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 118
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28S    
      
<400> 118
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Ser Arg Gly Arg Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 119
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31A    
      
<400> 119
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Ala Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 120
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31H    
      
<400> 120
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly His Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 121
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31L     
      
<400> 121
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Leu Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 122
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31N    
      
<400> 122
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Asn Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 123
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic L34A    
      
<400> 123
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys
            20                  25                  30          
Lys Ala Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 124
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic L34S     
      
<400> 124
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys
            20                  25                  30          
Lys Ser Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 125
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/R31A    
      
<400> 125
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Ala Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 126
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/R31N    
      
<400> 126
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Asn Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 127
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/R31S    
      
<400> 127
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Ser Lys
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 128
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/L34A     
      
<400> 128
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Arg Lys
            20                  25                  30          
Lys Ala Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 129
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/L34S    
      
<400> 129
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Arg Lys
            20                  25                  30          
Lys Ser Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 130
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31N/L34A    
      
<400> 130
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Asn Lys
            20                  25                  30          
Lys Ala Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 131
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic R31N/L34S    
      
<400> 131
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Asn Lys
            20                  25                  30          
Lys Ser Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 132
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/R31N/L34A     
      
<400> 132
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Asn Lys
            20                  25                  30          
Lys Ala Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 133
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic K28Q/R31N/L34S   
      
<400> 133
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly Asn Lys
            20                  25                  30          
Lys Ser Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu
    50                  55                  60                  
Gly Gly Cys Glu Leu
65                  


<210> 134
<211> 71
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic     
      
<220> 
<223> NN Insertion

<400> 134
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Asn Asn Lys Arg Gly
            20                  25                  30          
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val
        35                  40                  45              
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu
    50                  55                  60                  
Glu Glu Gly Gly Cys Glu Leu
65                  70      


<210> 135
<211> 70
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<400> 135
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser
 1               5                  10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg
            20                  25                  30          
Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln
        35                  40                  45              
Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu
    50                  55                  60                  
Glu Gly Gly Cys Glu Leu
65                  70  


<210> 136
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<400> 136
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn
 1               5                  10                  15      
Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu
            20                  25                  30          
Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly
        35                  40                  45              
Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe
    50                  55                  60                  
Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro
65                  70                  75                  80  
Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys
                85                  90                  95      
Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
            100                 105             


<210> 137
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region

<400> 137
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 138
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14A

<400> 138
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Ala Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 139
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14H

<400> 139
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val His Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 140
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14L

<400> 140
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Leu Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 141
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic     
      
<220> 
<223> junction region K14Q

<400> 141
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 142
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14S

<400> 142
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Ser Arg Gly
 1               5                  10                  15      
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 143
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region R17A

<400> 143
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Ala Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 144
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region R17H

<400> 144
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
His Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 145
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region R17L

<400> 145
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Leu Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 146
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> junction region R17N

<400> 146
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Asn Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 147
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region L20A

<400> 147
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Arg Lys Lys Ala Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 148
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region L20S

<400> 148
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Arg Lys Lys Ser Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 149
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> junction region K14Q/R17A

<400> 149
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Ala Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 150
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> junction region K14Q/R17N

<400> 150
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Asn Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 151
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14Q/R17S

<400> 151
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Ser Lys Lys Leu Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 152
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> junction region K14Q/L20A

<400> 152
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Arg Lys Lys Ala Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 153
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic   
      
<220> 
<223> junction region K14Q/L20S

<400> 153
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Arg Lys Lys Ser Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 154
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region R17N/L20A

<400> 154
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Asn Lys Lys Ala Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 155
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic  
      
<220> 
<223> junction region R17N/L20S

<400> 155
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly
 1               5                  10                  15      
Asn Lys Lys Ser Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 156
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14Q/R17N/L20A

<400> 156
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Asn Lys Lys Ala Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 157
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> junction region K14Q/R17N/L20S

<400> 157
Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Gln Arg Gly
 1               5                  10                  15      
Asn Lys Lys Ser Leu Tyr Ile Phe Lys Gln
            20                  25      


<210> 158
<211> 36
<212> DNA
<213> Homo sapiens

<220> 
<223> spacer (IgG4hinge)

<400> 158
gaatctaagt acggaccgcc ctgcccccct tgccct                           36

<210> 159
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic    
      
<220> 
<223> CD3 Zeta

<400> 159
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         

