                         SEQUENCE LISTING

<110>  iQUR LIMITED
 
<120>  Vaccines based on hepatitis B core antigens

<130>  N403290WO

<150>  GB1421692.3
<151>  2014-12-05

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  639
<212>  DNA
<213>  Hepatitis B virus

<220>
<223>  HBcAg ayw subtype nucleotide and amino acid sequence


<220>
<221>  CDS
<222>  (1)..(639)

<400>  1
atg caa ctt ttt cac ctc tgc cta atc atc tct tgt tca tgt cct act         48
Met Gln Leu Phe His Leu Cys Leu Ile Ile Ser Cys Ser Cys Pro Thr           
1               5                   10                  15                

gtt caa gcc tcc aag ctg tgc ctt ggg tgg ctt tgg ggc atg gac atc         96
Val Gln Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp Ile           
            20                  25                  30                    

gac cct tat aaa gaa ttt gga gct act gtg gag tta ctc tcg ttt ttg        144
Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu           
        35                  40                  45                        

cct tct gac ttc ttt cct tca gta cga gat ctt cta gat acc gcc tca        192
Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser           
    50                  55                  60                            

gct ctg tat cgg gaa gcc tta gag tct cct gag cat tgt tca cct cac        240
Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His           
65                  70                  75                  80            

cat act gca ctc agg caa gca att ctt tgc tgg ggg gaa cta atg act        288
His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr           
                85                  90                  95                

cta gct acc tgg gtg ggt gtt aat ttg gaa gat cca gcg tct aga gac        336
Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp           
            100                 105                 110                   

cta gta gtc agt tat gtc aac act aat atg ggc cta aag ttc agg caa        384
Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gln           
        115                 120                 125                       

ctc ttg tgg ttt cac att tct tgt ctc act ttt gga aga gaa aca gtt        432
Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val           
    130                 135                 140                           

ata gag tat ttg gtg tct ttc gga gtg tgg att cgc act cct cca gct        480
Ile Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala           
145                 150                 155                 160           

tat aga cca cca aat gcc cct atc cta tca aca ctt ccg gag act act        528
Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr           
                165                 170                 175               

gtt gtt aga cga cga ggc agg tcc cct aga aga aga act ccc tcg cct        576
Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro           
            180                 185                 190                   

cgc aga cga agg tct caa tcg ccg cgt cgc aga aga tct caa tct cgg        624
Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg Arg Ser Gln Ser Arg           
        195                 200                 205                       

gaa tct caa tgt tag                                                    639
Glu Ser Gln Cys                                                           
    210                                                                   


<210>  2
<211>  212
<212>  PRT
<213>  Hepatitis B virus

<220>
<223>  HBcAg ayw subtype protein sequence

<400>  2

Met Gln Leu Phe His Leu Cys Leu Ile Ile Ser Cys Ser Cys Pro Thr 
1               5                   10                  15      


Val Gln Ala Ser Lys Leu Cys Leu Gly Trp Leu Trp Gly Met Asp Ile 
            20                  25                  30          


Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu 
        35                  40                  45              


Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser 
    50                  55                  60                  


Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His 
65                  70                  75                  80  


His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr 
                85                  90                  95      


Leu Ala Thr Trp Val Gly Val Asn Leu Glu Asp Pro Ala Ser Arg Asp 
            100                 105                 110         


Leu Val Val Ser Tyr Val Asn Thr Asn Met Gly Leu Lys Phe Arg Gln 
        115                 120                 125             


Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
    130                 135                 140                 


Ile Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala 
145                 150                 155                 160 


Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr 
                165                 170                 175     


Val Val Arg Arg Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
            180                 185                 190         


Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg Arg Ser Gln Ser Arg 
        195                 200                 205             


Glu Ser Gln Cys 
    210             



<210>  3
<211>  97
<212>  PRT
<213>  Influenza A virus

<220>
<223>  Strain A/34/PR8 M2 protein

<400>  3

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Cys Arg Cys Asn Gly Ser Ser Asp Pro Leu Thr Ile Ala Ala Asn Ile 
            20                  25                  30          


Ile Gly Ile Leu His Leu Thr Leu Trp Ile Leu Asp Arg Leu Phe Phe 
        35                  40                  45              


Lys Cys Ile Tyr Arg Arg Phe Lys Tyr Gly Leu Lys Gly Gly Pro Ser 
    50                  55                  60                  


Thr Glu Gly Val Pro Lys Ser Met Arg Glu Glu Tyr Arg Lys Glu Gln 
65                  70                  75                  80  


Gln Ser Ala Val Asp Ala Asp Asp Gly His Phe Val Ser Ile Glu Leu 
                85                  90                  95      


Glu 
    


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Possible linker sequence

<400>  4

Gly Gly Gly Gly Ser 
1               5   


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Possible linker sequence

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  6
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild-type M2e sequence

<400>  6

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Cys Arg Cys Asn Gly Ser Ser Asp 
            20                  


<210>  7
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M2e sequence, position 17 Cys to Ser substitution

<400>  7

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Ser Arg Cys Asn Gly Ser Ser Asp 
            20                  


<210>  8
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M2e sequence, positions 17 and 19 Cys to Ser substitution

<400>  8

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Ser Arg Ser Asn Gly Ser Ser Asp 
            20                  


<210>  9
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Universal M2e consensus sequence (Cys to Ser substitutions)

<400>  9

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Gly Trp Gly 
1               5                   10                  15      


Ser Lys Ser Asn Gly Ser Ser Asp 
            20                  


<210>  10
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant M2e sequence

<400>  10

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Glu Trp Glu 
1               5                   10                  15      


Ser Arg Ser Ser Gly Ser Ser Asp 
            20                  


<210>  11
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tandem core with three M2e sequences

<400>  11

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Gly Ser Gly 
65                  70                  75                  80  


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ser 
                85                  90                  95      


Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Gly Trp Gly Ser Lys 
            100                 105                 110         


Ser Asn Gly Ser Ser Asp Met Ser Leu Leu Thr Glu Val Glu Thr Pro 
        115                 120                 125             


Ile Arg Asn Glu Trp Gly Ser Arg Ser Asn Gly Ser Ser Asp Met Ser 
    130                 135                 140                 


Leu Leu Thr Glu Val Glu Thr Pro Thr Arg Asn Glu Trp Glu Ser Arg 
145                 150                 155                 160 


Ser Ser Gly Ser Ser Asp Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                165                 170                 175     


Gly Gly Gly Gly Ser Gly Arg Asp Pro Ala Ser Arg Asp Leu Val Val 
            180                 185                 190         


Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln Leu Leu Trp 
        195                 200                 205             


Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu Tyr 
    210                 215                 220                 


Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala Tyr Arg Pro 
225                 230                 235                 240 


Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr Val Val Gly 
                245                 250                 255     


Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Thr Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly 
        275                 280                 285             


Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser 
    290                 295                 300                 


Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu 
305                 310                 315                 320 


Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu Arg Gln Ala 
                325                 330                 335     


Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp Val Gly Asn 
            340                 345                 350         


Asn Leu Glu Phe Ala Gly Ala Ser Asp Pro Ala Ser Arg Asp Leu Val 
        355                 360                 365             


Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln Leu Leu 
    370                 375                 380                 


Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu 
385                 390                 395                 400 


Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala Tyr Arg 
                405                 410                 415     


Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr Val Val 
            420                 425                 430         


Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro 
        435                 440                 445             


Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg Arg Ser Gln Ser Arg 
    450                 455                 460                 


Glu Ser Gln Cys Leu Glu His His His His His His 
465                 470                 475     


<210>  12
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tandem core with a HA stalk in one copy of HBcAg

<400>  12

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Gly Ser Met 
65                  70                  75                  80  


Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu Glu Lys 
                85                  90                  95      


Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile 
            100                 105                 110         


Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr 
        115                 120                 125             


Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg 
    130                 135                 140                 


Ser Gln Leu Lys Asn Asn Ala Ser Gly Arg Asp Pro Ala Ser Arg Asp 
145                 150                 155                 160 


Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln 
                165                 170                 175     


Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
            180                 185                 190         


Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala 
        195                 200                 205             


Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr 
    210                 215                 220                 


Val Val Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Ser Gly Gly Ser Thr Met Asp Ile Asp Pro Tyr Lys 
                245                 250                 255     


Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe 
            260                 265                 270         


Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg 
        275                 280                 285             


Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu 
    290                 295                 300                 


Arg Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp 
305                 310                 315                 320 


Val Gly Asn Asn Leu Glu Phe Ala Gly Ala Ser Asp Pro Ala Ser Arg 
                325                 330                 335     


Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg 
            340                 345                 350         


Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr 
        355                 360                 365             


Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro 
    370                 375                 380                 


Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr 
385                 390                 395                 400 


Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg Arg Arg Thr 
                405                 410                 415     


Pro Ser Pro Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Arg Arg Ser 
            420                 425                 430         


Gln Ser Arg Glu Ser Gln Cys Leu Glu His His His His His His 
        435                 440                 445         


<210>  13
<211>  547
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tandem core with HA stalk and three M2e sequences

<400>  13

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Gly Ser Met 
65                  70                  75                  80  


Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu Glu Lys 
                85                  90                  95      


Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile 
            100                 105                 110         


Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr 
        115                 120                 125             


Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg 
    130                 135                 140                 


Ser Gln Leu Lys Asn Asn Ala Ser Gly Arg Asp Pro Ala Ser Arg Asp 
145                 150                 155                 160 


Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln 
                165                 170                 175     


Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
            180                 185                 190         


Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala 
        195                 200                 205             


Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr 
    210                 215                 220                 


Val Val Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Ser Gly Gly Ser Thr Met Asp Ile Asp Pro Tyr Lys 
                245                 250                 255     


Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe 
            260                 265                 270         


Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg 
        275                 280                 285             


Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu 
    290                 295                 300                 


Arg Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp 
305                 310                 315                 320 


Val Gly Asn Asn Leu Glu Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                325                 330                 335     


Ser Gly Gly Gly Gly Ser Met Ser Leu Leu Thr Glu Val Glu Thr Pro 
            340                 345                 350         


Thr Arg Asn Gly Trp Gly Ser Lys Ser Asn Gly Ser Ser Asp Met Ser 
        355                 360                 365             


Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser Arg 
    370                 375                 380                 


Ser Asn Gly Ser Ser Asp Met Ser Leu Leu Thr Glu Val Glu Thr Pro 
385                 390                 395                 400 


Thr Arg Asn Glu Trp Glu Ser Arg Ser Ser Gly Ser Ser Asp Gly Gly 
                405                 410                 415     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Asp 
            420                 425                 430         


Pro Ala Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly 
        435                 440                 445             


Leu Lys Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe 
    450                 455                 460                 


Gly Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile 
465                 470                 475                 480 


Arg Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr 
                485                 490                 495     


Leu Pro Glu Thr Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro 
            500                 505                 510         


Arg Arg Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gln Ser Pro Arg 
        515                 520                 525             


Arg Arg Arg Ser Gln Ser Arg Glu Ser Gln Cys Leu Glu His His His 
    530                 535                 540                 


His His His 
545         


<210>  14
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M2e sequence with OH group added at C-terminus


<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Addition of an OH group at the C-terminus

<400>  14

Met Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly 
1               5                   10                  15      


Cys Arg Cys Asn Gly Ser Ser Asp 
            20                  


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Balance alpha-helices in HBcAg

<400>  15

Ala Ala Ala Leu Ala Ala Ala 
1               5           


<210>  16
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Possible linker

<400>  16

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  


<210>  17
<211>  4807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The DNA sequence for VLP1

<400>  17
agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag       60

gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt      120

tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc      180

agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta      240

acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta      300

tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg      360

agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct      420

gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg      480

ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt      540

cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct      600

ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct      660

ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact      720

gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat      780

atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt      840

actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga      900

caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattgcc gtgatcactg      960

cagaaaaaaa tggacatcga cccatacaaa gagttcggag ctactgttga gttgttgtcc     1020

ttcttgcctt ccgacttctt cccatccgtt agagacttgt tggacactgc ttccgctttg     1080

tacagagaag ctttggaatc tccagagcac tgttctccac atcacactgc tttgagacag     1140

gctatcttgt gttggggtga gttgatgact ttggctactt gggttggtaa caatctagaa     1200

ggatccatga atacacagtt cacagcagta ggtaaagagt tcaaccacct ggaaaaaaga     1260

atagagaatt taaataaaaa agttgatgat ggtttcctgg acatttggac ttacaatgcc     1320

gaactgttgg ttctattgga aaatgaaaga actttggact accacgattc aaatgtgaag     1380

aacttatatg aaaaggtaag aagccagtta aaaaacaatg ccagcggccg cgatcctgct     1440

tcaagagatt tggttgttaa ttacgttaac actaacatgg gtttgaagat cagacagttg     1500

ttgtggttcc acatctcctg tttgactttc ggtagagaga ctgttttgga gtacttggtt     1560

tccttcggtg tttggatcag aactccacca gcttacagac caccaaacgc tccaattttg     1620

tccactttgc cagagactac tgttgttggt gggagctctg gtggttcagg tggatcaggt     1680

ggttccggtg gatctggtgg atcaggtggg tcgactatgg atatcgatcc ttacaaagaa     1740

tttggtgcta cagttgagtt gttgtctttt ttgccatctg atttttttcc atctgttaga     1800

gatttgttgg atacagcttc tgctttgtat agagaggctt tggagtcccc tgaacactgt     1860

tcacctcacc atacagcttt gagacaagct attttgtgtt ggggagaatt gatgacattg     1920

gctacatggg ttggaaacaa cttggaattc ggtggtggtg gtagcggagg tggtggttct     1980

ggtggtggtg gttcaatgag tcttctaacc gaggtcgaaa cgcctactag aaacggttgg     2040

gggtccaaat ccaacggttc aagtgatatg agtcttctaa ccgaggtcga aacgcctatc     2100

agaaacgaat gggggtccag atccaacggt tcaagtgata tgagtcttct aaccgaggtc     2160

gaaacgccta ctagaaacga atgggaatcc agatcctccg gttcaagtga tggtggtggt     2220

ggaagcggag gtggtggttc tggtggtggt ggtgctagcg atccagcttc cagagacttg     2280

gttgttaatt atgttaacac aaatatggga ttgaaaatta gacagttgtt gtggtttcat     2340

atttcttgtt tgacatttgg aagagaaaca gttttggaat atttggtttc ttttggagtt     2400

tggattagaa cacctcctgc ttatagacct cctaatgctc ctatcttgtc tacattgcct     2460

gaaacaacag ttgttagaag aagagacaga ggtagatccc caagaagaag aactccatct     2520

cctagaagaa gaagatccca gtcaccacgt agaagaagat cacaatccag agaatcccag     2580

tgttaaggcg cctgatgact cgagggccca ccggtcttgc tagattctaa tcaagaggat     2640

gtcagaatgc catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa     2700

cctatatagt ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga     2760

tcagcctatc tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt     2820

ttgatgtttt tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct     2880

tcgtttgtgc ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc     2940

ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca     3000

tactaaattt tccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg     3060

gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt     3120

ttatcacgtt tctttttctt gaaatttttt tttttagttt ttttctcttt cagtgacctc     3180

cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt catttttctt     3240

gttctattac aacttttttt acttcttgtt cattagaaag aaagcatagc aatctaatct     3300

aaggggcggt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa     3360

ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga     3420

cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga     3480

ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga     3540

ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta     3600

cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac     3660

cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg     3720

cgtgcacttc gtggccgagg agcaggactg acacgtccga cggcggccca cgggtcccag     3780

gcctcggaga tccgtccccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct     3840

tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg     3900

aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat     3960

ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa     4020

ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctg gagaccaaca     4080

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     4140

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     4200

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     4260

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     4320

tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     4380

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     4440

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     4500

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     4560

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     4620

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     4680

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     4740

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     4800

tgagatc                                                               4807


<210>  18
<211>  538
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tandem core, HA stalk, 3 copies M2e, linkers

<400>  18

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Gly Ser Met 
65                  70                  75                  80  


Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu Glu Lys 
                85                  90                  95      


Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile 
            100                 105                 110         


Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr 
        115                 120                 125             


Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg 
    130                 135                 140                 


Ser Gln Leu Lys Asn Asn Ala Ser Gly Arg Asp Pro Ala Ser Arg Asp 
145                 150                 155                 160 


Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln 
                165                 170                 175     


Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val 
            180                 185                 190         


Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala 
        195                 200                 205             


Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr 
    210                 215                 220                 


Val Val Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Ser Gly Gly Ser Thr Met Asp Ile Asp Pro Tyr Lys 
                245                 250                 255     


Glu Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe 
            260                 265                 270         


Phe Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg 
        275                 280                 285             


Glu Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu 
    290                 295                 300                 


Arg Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp 
305                 310                 315                 320 


Val Gly Asn Asn Leu Glu Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                325                 330                 335     


Ser Gly Gly Gly Gly Ser Met Ser Leu Leu Thr Glu Val Glu Thr Pro 
            340                 345                 350         


Thr Arg Asn Gly Trp Gly Ser Lys Ser Asn Gly Ser Ser Asp Met Ser 
        355                 360                 365             


Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Ser Arg 
    370                 375                 380                 


Ser Asn Gly Ser Ser Asp Met Ser Leu Leu Thr Glu Val Glu Thr Pro 
385                 390                 395                 400 


Thr Arg Asn Glu Trp Glu Ser Arg Ser Ser Gly Ser Ser Asp Gly Gly 
                405                 410                 415     


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Asp Pro 
            420                 425                 430         


Ala Ser Arg Asp Leu Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu 
        435                 440                 445             


Lys Ile Arg Gln Leu Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly 
    450                 455                 460                 


Arg Glu Thr Val Leu Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg 
465                 470                 475                 480 


Thr Pro Pro Ala Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu 
                485                 490                 495     


Pro Glu Thr Thr Val Val Arg Arg Arg Asp Arg Gly Arg Ser Pro Arg 
            500                 505                 510         


Arg Arg Thr Pro Ser Pro Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg 
        515                 520                 525             


Arg Arg Ser Gln Ser Arg Glu Ser Gln Cys 
    530                 535             


<210>  19
<211>  4495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The DNA sequence for VLP2

<400>  19
agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag       60

gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt      120

tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc      180

agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta      240

acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta      300

tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg      360

agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct      420

gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg      480

ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt      540

cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct      600

ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct      660

ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact      720

gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat      780

atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt      840

actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga      900

caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattgcc gtgatcactg      960

cagaaaaaaa tggacatcga cccatacaaa gagttcggag ctactgttga gttgttgtcc     1020

ttcttgcctt ccgacttctt cccatccgtt agagacttgt tggacactgc ttccgctttg     1080

tacagagaag ctttggaatc tccagagcac tgttctccac atcacactgc tttgagacag     1140

gctatcttgt gttggggtga gttgatgact ttggctactt gggttggtaa caatctagaa     1200

ggatccagaa tccaagattt ggaaaagtac gttgaagata ctaagattga tttgtggtct     1260

tacaacgctg aattgttggt tgctttggaa aaccaacata ctattgattt gactgattct     1320

gaaatgaaca agttgtttga aaagactaga agacaattga gagaaaacgc tagcggccgc     1380

gatcctgctt caagagattt ggttgttaat tacgttaaca ctaacatggg tttgaagatc     1440

agacagttgt tgtggttcca catctcctgt ttgactttcg gtagagagac tgttttggag     1500

tacttggttt ccttcggtgt ttggatcaga actccaccag cttacagacc accaaacgct     1560

ccaattttgt ccactttgcc agagactact gttgttggtg ggagctctgg tggttcaggt     1620

ggatcaggtg gttccggtgg atctggtgga tcaggtgggt cgactatgga tatcgatcct     1680

tacaaagaat ttggtgctac agttgagttg ttgtcttttt tgccatctga tttttttcca     1740

tctgttagag atttgttgga tacagcttct gctttgtata gagaggcttt ggagtcccct     1800

gaacactgtt cacctcacca tacagctttg agacaagcta ttttgtgttg gggagaattg     1860

atgacattgg ctacatgggt tggaaacaac ttggaattcg gttccggatc aggtggtgga     1920

aagggtggtg gatcaggttc tgctagcgat ccagcttcca gagacttggt tgttaattat     1980

gttaacacaa atatgggatt gaaaattaga cagttgttgt ggtttcatat ttcttgtttg     2040

acatttggaa gagaaacagt tttggaatat ttggtttctt ttggagtttg gattagaaca     2100

cctcctgctt atagacctcc taatgctcct atcttgtcta cattgcctga aacaacagtt     2160

gttagaagaa gagacagagg tagatcccca agaagaagaa ctccatctcc tagaagaaga     2220

agatcccagt caccacgtag aagaagatca caatccagag aatcccagtg ttaaggcgcc     2280

tgatgactcg agggcccacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca     2340

tttgcctgag agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat     2400

aggatttttt ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc     2460

gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc     2520

ttggtatttc ccactcctct tcagagtaca gaagattaag tgagaccttc gtttgtgcgg     2580

atcccccaca caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc     2640

tcggactccg cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc     2700

cctctttctt cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa     2760

gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc     2820

tttttcttga aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta     2880

agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa     2940

ctttttttac ttcttgttca ttagaaagaa agcatagcaa tctaatctaa ggggcggtgt     3000

tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta     3060

aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc     3120

ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc     3180

cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc     3240

ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc     3300

ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga     3360

gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt     3420

ggccgaggag caggactgac acgtccgacg gcggcccacg ggtcccaggc ctcggagatc     3480

cgtccccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc     3540

ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc     3600

ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc     3660

ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga     3720

aggttttggg acgctcgaag gctttaattt gcaagctgga gaccaacatg tgagcaaaag     3780

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     3840

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     3900

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     3960

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     4020

aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     4080

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     4140

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     4200

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     4260

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     4320

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     4380

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     4440

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agatc          4495


<210>  20
<211>  434
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tandem core, HA stalk, null insert

<400>  20

Met Asp Ile Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Glu Leu Leu 
1               5                   10                  15      


Ser Phe Leu Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Leu Leu Asp 
            20                  25                  30          


Thr Ala Ser Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Glu His Cys 
        35                  40                  45              


Ser Pro His His Thr Ala Leu Arg Gln Ala Ile Leu Cys Trp Gly Glu 
    50                  55                  60                  


Leu Met Thr Leu Ala Thr Trp Val Gly Asn Asn Leu Glu Gly Ser Arg 
65                  70                  75                  80  


Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu Trp 
                85                  90                  95      


Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile 
            100                 105                 110         


Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Arg 
        115                 120                 125             


Gln Leu Arg Glu Asn Ala Ser Gly Arg Asp Pro Ala Ser Arg Asp Leu 
    130                 135                 140                 


Val Val Asn Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln Leu 
145                 150                 155                 160 


Leu Trp Phe His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu 
                165                 170                 175     


Glu Tyr Leu Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala Tyr 
            180                 185                 190         


Arg Pro Pro Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr Val 
        195                 200                 205             


Val Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
    210                 215                 220                 


Ser Gly Gly Ser Gly Gly Ser Thr Met Asp Ile Asp Pro Tyr Lys Glu 
225                 230                 235                 240 


Phe Gly Ala Thr Val Glu Leu Leu Ser Phe Leu Pro Ser Asp Phe Phe 
                245                 250                 255     


Pro Ser Val Arg Asp Leu Leu Asp Thr Ala Ser Ala Leu Tyr Arg Glu 
            260                 265                 270         


Ala Leu Glu Ser Pro Glu His Cys Ser Pro His His Thr Ala Leu Arg 
        275                 280                 285             


Gln Ala Ile Leu Cys Trp Gly Glu Leu Met Thr Leu Ala Thr Trp Val 
    290                 295                 300                 


Gly Asn Asn Leu Glu Phe Gly Ser Gly Ser Gly Gly Gly Lys Gly Gly 
305                 310                 315                 320 


Gly Ser Gly Ser Ala Ser Asp Pro Ala Ser Arg Asp Leu Val Val Asn 
                325                 330                 335     


Tyr Val Asn Thr Asn Met Gly Leu Lys Ile Arg Gln Leu Leu Trp Phe 
            340                 345                 350         


His Ile Ser Cys Leu Thr Phe Gly Arg Glu Thr Val Leu Glu Tyr Leu 
        355                 360                 365             


Val Ser Phe Gly Val Trp Ile Arg Thr Pro Pro Ala Tyr Arg Pro Pro 
    370                 375                 380                 


Asn Ala Pro Ile Leu Ser Thr Leu Pro Glu Thr Thr Val Val Arg Arg 
385                 390                 395                 400 


Arg Asp Arg Gly Arg Ser Pro Arg Arg Arg Thr Pro Ser Pro Arg Arg 
                405                 410                 415     


Arg Arg Ser Gln Ser Pro Arg Arg Arg Arg Ser Gln Ser Arg Glu Ser 
            420                 425                 430         


Gln Cys 
        

<210>  21
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Null insert

<400>  21

Gly Ser Gly Ser Gly Gly Gly Lys Gly Gly Gly Ser Gly Ser 
1               5                   10                  


<210>  22
<211>  72
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA Stalk 403-474 (from H1N1)

<400>  22

Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu Glu 
1               5                   10                  15      


Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp 
            20                  25                  30          


Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg 
        35                  40                  45              


Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val 
    50                  55                  60                  


Arg Ser Gln Leu Lys Asn Asn Ala 
65                  70          


<210>  23
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA long alpha helix (LAH) 420-474 (from H1N1)

<400>  23

Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp Ile 
1               5                   10                  15      


Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr 
            20                  25                  30          


Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val Arg 
        35                  40                  45              


Ser Gln Leu Lys Asn Asn Ala 
    50                  55  


<210>  24
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA long alpha helix (LAH) 421-475 (from H3N2)

<400>  24

Arg Ile Gln Asp Leu Glu Lys Tyr Val Glu Asp Thr Lys Ile Asp Leu 
1               5                   10                  15      


Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr 
            20                  25                  30          


Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg 
        35                  40                  45              


Arg Gln Leu Arg Glu Asn Ala 
    50                  55  


