                         SEQUENCE LISTING

<110>  кŵHangzhou Joingenome
 
<120>  ΰϱ־ϸ𤸽Ӽ;MCAM AS LIVER CANCER DIAGNOSIS MARKER AND USE THEREOF

<130>  iarkip20141126

<160>  46    

<170>  PatentIn version 3.3

<210>  1
<211>  3332
<212>  DNA
<213>  ˣHomo sapiens

<400>  1
acttggctct cgccctccgg ccgggaagca tggggcttcc caggctggtc tgcgccttct     60

tgctcgccgc ctgctgctgc tgtcctcgcg tcgcgggtgt gcccggagag gctgagcagc    120

ctgcgcctga gctggtggag gtggaagtgg gcagcacagc ccttctgaag tgcggcctct    180

cccagtccca aggcaacctc agccatgtcg actggttttc tgtccacaag gagaagcgga    240

cgctcatctt ccgtgtgcgc cagggccagg gccagagcga acctggggag tacgagcagc    300

ggctcagcct ccaggacaga ggggctactc tggccctgac tcaagtcacc ccccaagacg    360

agcgcatctt cttgtgccag ggcaagcgcc ctcggtccca ggagtaccgc atccagctcc    420

gcgtctacaa agctccggag gagccaaaca tccaggtcaa ccccctgggc atccctgtga    480

acagtaagga gcctgaggag gtcgctacct gtgtagggag gaacgggtac cccattcctc    540

aagtcatctg gtacaagaat ggccggcctc tgaaggagga gaagaaccgg gtccacattc    600

agtcgtccca gactgtggag tcgagtggtt tgtacacctt gcagagtatt ctgaaggcac    660

agctggttaa agaagacaaa gatgcccagt tttactgtga gctcaactac cggctgccca    720

gtgggaacca catgaaggag tccagggaag tcaccgtccc tgttttctac ccgacagaaa    780

aagtgtggct ggaagtggag cccgtgggaa tgctgaagga aggggaccgc gtggaaatca    840

ggtgtttggc tgatggcaac cctccaccac acttcagcat cagcaagcag aaccccagca    900

ccagggaggc agaggaagag acaaccaacg acaacggggt cctggtgctg gagcctgccc    960

ggaaggaaca cagtgggcgc tatgaatgtc agggcctgga cttggacacc atgatatcgc   1020

tgctgagtga accacaggaa ctactggtga actatgtgtc tgacgtccga gtgagtcccg   1080

cagcccctga gagacaggaa ggcagcagcc tcaccctgac ctgtgaggca gagagtagcc   1140

aggacctcga gttccagtgg ctgagagaag agacaggcca ggtgctggaa agggggcctg   1200

tgcttcagtt gcatgacctg aaacgggagg caggaggcgg ctatcgctgc gtggcgtctg   1260

tgcccagcat acccggcctg aaccgcacac agctggtcaa cgtggccatt tttggccccc   1320

cttggatggc attcaaggag aggaaggtgt gggtgaaaga gaatatggtg ttgaatctgt   1380

cttgtgaagc gtcagggcac ccccggccca ccatctcctg gaacgtcaac ggcacggcaa   1440

gtgaacaaga ccaagatcca cagcgagtcc tgagcaccct gaatgtcctc gtgaccccgg   1500

agctgttgga gacaggtgtt gaatgcacgg cctccaacga cctgggcaaa aacaccagca   1560

tcctcttcct ggagctggtc aatttaacca ccctcacacc agactccaac acaaccactg   1620

gcctcagcac ttccactgcc agtcctcata ccagagccaa cagcacctcc acagagagaa   1680

agctgccgga gccggagagc cggggcgtgg tcatcgtggc tgtgattgtg tgcatcctgg   1740

tcctggcggt gctgggcgct gtcctctatt tcctctataa gaagggcaag ctgccgtgca   1800

ggcgctcagg gaagcaggag atcacgctac ccccgtctcg taagagcgaa cttgtagttg   1860

aagttaagtc agataagctc ccagaagaga tgggcctcct gcagggcagc agcggtgaca   1920

agagggctcc gggagaccag ggagagaaat acatcgatct gaggcattag ccccgaatca   1980

cttcagctcc cttccctgcc tggaccattc ccagctccct gctcactctt ctctcagcca   2040

aagcctccaa agggactaga gagaagcctc ctgctcccct cgcctgcaca ccccctttca   2100

gagggccact gggttaggac ctgaggacct cacttggccc tgcaaggccc gcttttcagg   2160

gaccagtcca ccaccatctc ctccacgttg agtgaagctc atcccaagca aggagcccca   2220

gtctcccgag cgggtaggag agtttcttgc agaacgtgtt ttttctttac acacattatg   2280

gctgtaaata cctggctcct gccagcagct gagctgggta gcctctctga gctggtttcc   2340

tgccccaaag gctggcttcc accatccagg tgcaccactg aagtgaggac acaccggagc   2400

caggcgcctg ctcatgttga agtgcgctgt tcacacccgc tccggagagc accccagcag   2460

catccagaag cagctgcagt gttgctgcca ccaccctcct gtctgcctct tcaaagtctc   2520

ctgtgacatt ttttctttgg tcagaagcca ggaactggtg tcattcctta aaagatacgt   2580

gccggggcca ggtgtggtgg ctcacgcctg taatcccagc actttgggag gccgaggcgg   2640

gcggatcaca aagtcaggac gagaccatcc tggctaacac ggtgaaaccc tgtctctact   2700

aaaaatacaa aaaaaaatta gctaggcgta gtggttggca cctatagtcc cagctactcg   2760

gaaggctgaa gcaggagaat ggtatgaatc caggaggtgg agcttgcagt gagccgagac   2820

cgtgccactg cactccagcc tgggcaacac agcgagactc cgtctcgagg aaaaaaaaag   2880

aaaagatacg tgcctgcggt gaggaagctg ggcgctgttt tcgagttcag gtgaattagc   2940

ctcaatcccc cgtgttcact tggctcccat agccctcttg atggatcacg taaaactgaa   3000

aggcagcggg gagcagacaa agatgaggtc tacactgtcc ttcatgggga ttaaagctat   3060

ggttatatta gcaccaaact tctacaaacc aagctcaggg ccccaaccct agaagggccc   3120

aaatgagaga atggtactta gggatggaaa acgggcctgg ctagagcttc gggtgtgtgt   3180

gtctgtctgt gtgtatgcat acatatgtgt gtatatatgg ttttgtcagg tgtgtaaatt   3240

tgcaaattgt ttcctttata tatgtatgta tatatatata tgaaaatata tatatatatg   3300

aaaaataaag cttaattgtc ccagaaatca ta                                 3332


<210>  2
<211>  646
<212>  PRT
<213>  ˣHomo sapiens

<400>  2

Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      


Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          


Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              


Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  


Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  


Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      


Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         


Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             


Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 


Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 


Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     


Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         


Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             


Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 


Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 


Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     


Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         


Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             


Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 


Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 


Gln Gly Leu Asp Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     


Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         


Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             


Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Gly Gln 
    370                 375                 380                 


Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 


Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     


Leu Asn Arg Thr Gln Leu Val Asn Val Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         


Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             


Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 


Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 


Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     


Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         


Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             


Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 


Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu Pro Glu Ser Arg Gly Val 
545                 550                 555                 560 


Val Ile Val Ala Val Ile Val Cys Ile Leu Val Leu Ala Val Leu Gly 
                565                 570                 575     


Ala Val Leu Tyr Phe Leu Tyr Lys Lys Gly Lys Leu Pro Cys Arg Arg 
            580                 585                 590         


Ser Gly Lys Gln Glu Ile Thr Leu Pro Pro Ser Arg Lys Ser Glu Leu 
        595                 600                 605             


Val Val Glu Val Lys Ser Asp Lys Leu Pro Glu Glu Met Gly Leu Leu 
    610                 615                 620                 


Gln Gly Ser Ser Gly Asp Lys Arg Ala Pro Gly Asp Gln Gly Glu Lys 
625                 630                 635                 640 


Tyr Ile Asp Leu Arg His 
                645     


<210>  3
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  3
atcggctagc atggggcttc ccaggctggt ctgcg                                35


<210>  4
<211>  64
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  4
atcggcggcc gctagagcgt agtctgggac gtcgtatggg taatgcctca gatcgatgta     60

tttc                                                                  64


<210>  5
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  5
atgcgatatc cggcgagcaa gaaggcgcag accag                                35


<210>  6
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  6
atgcggtacc agcctctggc ctctaagaag agctg                                35


<210>  7
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  7
atgcggtacc gacccaggga gaagccggcg ttttg                                35


<210>  8
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  8
atgcggtacc ggcagcggca gtggtgcaat cgttc                                35


<210>  9
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  9
atgcggtacc ccgaggacca ggtccgctac tgctc                                35


<210>  10
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  10
atgcggtacc ggggcggggc gggcccgggc gctcc                                35


<210>  11
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  11
atgcggtacc ccccctcccc cgcccccctg acgtc                                35


<210>  12
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  12
atgcggtacc tccgggcccc ctcccccgcc cccct                                35


<210>  13
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  13
atgcggtacc tgacgtcagc ccccggcagc ctcga                                35


<210>  14
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  14
atgcggtacc agcccccggc agcctcgagc tgctc                                35


<210>  15
<211>  35
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  15
aggctgccgg gggctagggg ggcgggggag ggggc                                35


<210>  16
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  Primers used for plasmids construction

<400>  16
agcccccggc agcctcgagc tgctc                                           25


<210>  17
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  17
ggcaaggtgg aacagttatc tccag                                           25


<210>  18
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  18
catcttctag ttggtctgtc tccgc                                           25


<210>  19
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  19
gcttaccaaa ggatagtgcg atgtt                                           25


<210>  20
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  20
tgtaaaatct gccaccaatt cctgc                                           25


<210>  21
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  21
tgtttttgga atggagcctc gcatc                                           25


<210>  22
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  22
ctgctgtaca ctggctgtcc acaag                                           25


<210>  23
<211>  20
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  23
ttgcatgacc tgaaacggga                                                 20


<210>  24
<211>  20
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  24
gtcttgttca cttgccgtgc                                                 20


<210>  25
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  25
ttaaagacag attctacatg cgcac                                           25


<210>  26
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  26
cctttgaaaa accggacttc atctc                                           25


<210>  27
<211>  20
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  27
atcatccctg cctctactgg                                                 20


<210>  28
<211>  20
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  28
gtcaggtcca ccactgacac                                                 20


<210>  29
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  29
agttatgtgt tgacatccag atcac                                           25


<210>  30
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  qPCRPrimers for qPCR

<400>  30
gaagctgctt tttacctttg acacc                                           25


<210>  31
<211>  27
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  RNA-IPPrimers for RNA-IP

<400>  31
tggaacagtt atctccagaa gaagaag                                         27


<210>  32
<211>  22
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  RNA-IPPrimers for RNA-IP

<400>  32
tcggtgagct gccaggatga ac                                              22


<210>  33
<211>  23
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  RNA-IPPrimers for RNA-IP

<400>  33
gcgctgttga cagcggcgga aag                                             23


<210>  34
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  RNA-IPPrimers for RNA-IP

<400>  34
ctctccgccg ccttctggtc tttac                                           25


<210>  35
<211>  23
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  ChIPPrimers for ChIP

<400>  35
gaccccgaga cacctgtcac tac                                             23


<210>  36
<211>  25
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  ChIPPrimers for ChIP

<400>  36
tcccggccgg agggcgagag ccaag                                           25


<210>  37
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  37
ccggaagctt tgagttctga catccctcga gggatgtcag aactcaaagc tttttttc       58


<210>  38
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  38
aattcaaaaa aagctttgag ttctgacatc cctcgaggga tgtcagaact caaagctt       58


<210>  39
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  39
ccggctggtc agagatactt cttaactcga gttaagaagt atctctgacc agtttttc       58


<210>  40
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  40
aattcaaaaa ctggtcagag atacttctta actcgagtta agaagtatct ctgaccag       58


<210>  41
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  41
ccggaactcc tcaagaatga tggcactcga gtgccatcat tcttgaggag tttttttg       58


<210>  42
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  42
aattcaaaaa aactcctcaa gaatgatggc actcgagtgc catcattctt gaggagtt       58


<210>  43
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  43
ccggaaccct caacaacttc tctgtctcga gacagagaag ttgttgaggg tttttttg       58


<210>  44
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  44
aattcaaaaa aaccctcaac aacttctctg tctcgagaca gagaagttgt tgagggtt       58


<210>  45
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  45
ccggaagcca tgaagatcct caagactcga gtcttgagga tcttcatggc tttttttg       58


<210>  46
<211>  58
<212>  DNA
<213>  ˹УArtificial

<220>
<223>  shRNAsPrimers for shRNAs

<400>  46
aattcaaaaa aagccatgaa gatcctcaag actcgagtct tgaggatctt catggctt       58


