			SEQUENCE LISTING

<110> CODEXIS, INC.
      ALVIZO, OSCAR
      MAYO, MELISSA ANN
      MOORE, JEFFREY C.
 
<120> ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
 
<130> CX2-145USP1
 
<140> 
<141> 
 
<150> 62/084,037
<151> 2014-11-25
 
<160> 102
 
<210> 1
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 1
atgatcgagt caaaaacata cgccgttttg ggaatgggaa gcgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtattct tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 2
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 2
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Ser Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 3
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 3
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcaacca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaattt agaagctgta 600
atacacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcaa atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtattct tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 4
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 4
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Thr Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Lys Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 5
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 5
atgatcgagt caaaaacata cgccgttttg ggaatgggaa gcgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcaacca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaga cttactcttt 420
gtagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaattt agaagctgta 600
atacacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacaat tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 6
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 6
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Ser Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Thr Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asp Leu Leu Phe Val Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 7
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 7
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cctgctcttt 420
gaagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 8
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 8
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Leu Leu Phe Glu Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 9
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 9
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcaacca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 10
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 10
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Thr Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 11
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 11
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaga cttactcttt 420
gaagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggtcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 12
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 12
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asp Leu Leu Phe Glu Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 13
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 13
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cttactcttt 420
gaagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 14
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 14
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Leu Leu Phe Glu Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 15
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 15
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaga cttactcttt 420
gaagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacaat tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 16
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 16
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asp Leu Leu Phe Glu Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 17
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 17
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cctgctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
ggtcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 18
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 18
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Leu Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Gly His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 19
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 19
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaga tctgctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 20
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 20
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asp Leu Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 21
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 21
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 22
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 22
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 23
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 23
atgatcgagt caaaaacata cgccgttttg ggaatgggaa gcgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtagctagat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
atacacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtattct tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 24
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 24
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Ser Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 25
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 25
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa ctttctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 26
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 26
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Phe Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 27
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 27
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtagctagat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtattct tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 28
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 28
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 29
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 29
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaga cttactcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
ggccacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggtcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 30
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 30
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asp Leu Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Gly His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 31
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 31
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 32
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 32
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 33
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 33
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cggtctcttt 420
gtagctaaat ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtacaat tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 34
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 34
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Lys Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 35
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 35
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcaacaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cctgctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 36
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 36
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Thr Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Leu Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 37
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 37
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcaa aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cctgctcttt 420
gtcgctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tgggacctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 38
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 38
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Leu Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Gly Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 39
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 39
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 40
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 40
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 41
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 41
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 42
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 42
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 43
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 43
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 44
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 44
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 45
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 45
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 46
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 46
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 47
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 47
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 48
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 48
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 49
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 49
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 50
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 50
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 51
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 51
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgta gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 52
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 52
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Ser Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 53
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 53
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 54
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 54
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 55
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 55
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 56
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 56
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 57
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 57
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 58
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 58
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 59
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 59
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 60
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 60
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 61
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 61
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
atggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 62
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 62
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Met Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 63
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 63
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 64
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 64
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 65
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 65
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagatgc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 66
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 66
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Met Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 67
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 67
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 68
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 68
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 69
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 69
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gacagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 70
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 70
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 71
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 71
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 72
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 72
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 73
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 73
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 74
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 74
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 75
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 75
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 76
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 76
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 77
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 77
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 78
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 78
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 79
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 79
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 80
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 80
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 81
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 81
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 82
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 82
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 83
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 83
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 84
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 84
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 85
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 85
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 86
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 86
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 87
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 87
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 88
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 88
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 89
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 89
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 90
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 90
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 91
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 91
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 92
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 92
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 93
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 93
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 94
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 94
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 95
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 95
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctgg cagccttaac acggtacagt tcaaaaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 96
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 96
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Gly Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Lys Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 97
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 97
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtatttt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 98
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 98
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Phe Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 99
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 99
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggctgcgg acatcgatgc tcagagaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atgggttaac 780
gatgtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctaa aagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 100
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 100
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Ala Asp Ile Asp Ala Gln Arg Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Val Asn Asp Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Lys Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

 
<210> 101
<211> 1077
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 101
atgatcgagt caaaaacata cgccgttttg ggaatgggaa acgggggtca cgcatttgcc 60
gcctatttag cattgaaggg ccagcgtgta ctggcttggg acatcgatgc tcagaaaatc 120
aaagagattc aggaccgtgg agccatcata gcggaaggac caggacttgc tgggactgct 180
cacccggatc ttgtgacctc agatataggt ttggcggtta aagacgctga tgtgatactg 240
attaccgtgc cggccatcca ccacgcttcc attgcagcca aaatcgccag ttacatttcc 300
gagggtcagt tgattatcct tagccctggg gcgacgggtg gagcgctgga attccggaaa 360
atcctgcgtg aaaatggtgc gccggaggta acgatcgggg aaacctcaaa cgggctcttt 420
gtagctcggt ccgagagacc aggacaggtt acggtatatt ttatacagct ggcccaggac 480
tttgcatgtc tcccagcggc aatggctgga tgggccctgg aacagatagg gtcggttctt 540
cctcaatacc aggctgtcga aaacgtgttg catacttcgc tgactaatct ggaagctgta 600
attcacccgc tgactaatct gttaaacgct gctcgttgtg agtctggtac cccattcgat 660
tattataccg aggggattac gccatcagtc ggggcgcttt gcgagaaagt ggaccaggaa 720
cgcatagcaa tcgcaaaggc tttcgattta aacgttccgt cggtcggcga atggattaac 780
gaagtttgtg gccagacccc ggccacaatt tatgaagcga tgcagggtct gccttttctg 840
aggggctgga tggttcctac cagccttaac acggtacagt tcagcaacga tgtaagcacc 900
ggactggtac cgttatccga actgggtcgg gtggtgaatg tgcctacccc acttatcgat 960
gctgttcttc tgttgatcag tgaactggtt gacgtggatt tcagaaagga aggtcgtacc 1020
ttggaaaaat taggtctgag cggacttacc gccgcggaaa taagaagtgc tgttgag    1077

 
<210> 102
<211> 359
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Opine dehydrogease Variant from Arthrobactor sp. strain 1C 
 
<400> 102
Met Ile Glu Ser Lys Thr Tyr Ala Val Leu Gly Met Gly Asn Gly Gly 
1               5                   10                  15      
His Ala Phe Ala Ala Tyr Leu Ala Leu Lys Gly Gln Arg Val Leu Ala 
            20                  25                  30          
Trp Asp Ile Asp Ala Gln Lys Ile Lys Glu Ile Gln Asp Arg Gly Ala 
        35                  40                  45              
Ile Ile Ala Glu Gly Pro Gly Leu Ala Gly Thr Ala His Pro Asp Leu 
    50                  55                  60                  
Val Thr Ser Asp Ile Gly Leu Ala Val Lys Asp Ala Asp Val Ile Leu 
65                  70                  75                  80  
Ile Thr Val Pro Ala Ile His His Ala Ser Ile Ala Ala Lys Ile Ala 
                85                  90                  95      
Ser Tyr Ile Ser Glu Gly Gln Leu Ile Ile Leu Ser Pro Gly Ala Thr 
            100                 105                 110         
Gly Gly Ala Leu Glu Phe Arg Lys Ile Leu Arg Glu Asn Gly Ala Pro 
        115                 120                 125             
Glu Val Thr Ile Gly Glu Thr Ser Asn Gly Leu Phe Val Ala Arg Ser 
    130                 135                 140                 
Glu Arg Pro Gly Gln Val Thr Val Tyr Phe Ile Gln Leu Ala Gln Asp 
145                 150                 155                 160 
Phe Ala Cys Leu Pro Ala Ala Met Ala Gly Trp Ala Leu Glu Gln Ile 
                165                 170                 175     
Gly Ser Val Leu Pro Gln Tyr Gln Ala Val Glu Asn Val Leu His Thr 
            180                 185                 190         
Ser Leu Thr Asn Leu Glu Ala Val Ile His Pro Leu Thr Asn Leu Leu 
        195                 200                 205             
Asn Ala Ala Arg Cys Glu Ser Gly Thr Pro Phe Asp Tyr Tyr Thr Glu 
    210                 215                 220                 
Gly Ile Thr Pro Ser Val Gly Ala Leu Cys Glu Lys Val Asp Gln Glu 
225                 230                 235                 240 
Arg Ile Ala Ile Ala Lys Ala Phe Asp Leu Asn Val Pro Ser Val Gly 
                245                 250                 255     
Glu Trp Ile Asn Glu Val Cys Gly Gln Thr Pro Ala Thr Ile Tyr Glu 
            260                 265                 270         
Ala Met Gln Gly Leu Pro Phe Leu Arg Gly Trp Met Val Pro Thr Ser 
        275                 280                 285             
Leu Asn Thr Val Gln Phe Ser Asn Asp Val Ser Thr Gly Leu Val Pro 
    290                 295                 300                 
Leu Ser Glu Leu Gly Arg Val Val Asn Val Pro Thr Pro Leu Ile Asp 
305                 310                 315                 320 
Ala Val Leu Leu Leu Ile Ser Glu Leu Val Asp Val Asp Phe Arg Lys 
                325                 330                 335     
Glu Gly Arg Thr Leu Glu Lys Leu Gly Leu Ser Gly Leu Thr Ala Ala 
            340                 345                 350         
Glu Ile Arg Ser Ala Val Glu                                     
        355                                                     

