                         SEQUENCE LISTING

<110>  Danziger Innovations Ltd.
       VAINSTEIN, Alexander
       MARTON, Ira
       HONIG, Arik
       MARHEVKA, Elena
 
<120>  NUCLEIC ACID CONSTRUCTS FOR GENOME EDITING

<130>  64725

<150>  US 62/085,292
<151>  2014-11-27

<160>  107   

<170>  PatentIn version 3.5

<210>  1
<211>  103
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of nptII(k)-sgRNA

<400>  1
gctgtcagcg caggggcgcc gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  2
<211>  103
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of PDS sgRNA

<400>  2
gccgttaatt tgagagtcca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  3
<211>  167
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of QQR sgRNA cassette

<400>  3
gatatcaagc ttgttaacga ggtaattctt cccctcagga ggttttagag ctagaaatag       60

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt      120

ttttctcgag ggatttaagg acgtgaactc tgttgagatc ttacgta                    167


<210>  4
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of Hammerhead (HH) ribozyme

<400>  4
ggccatctga tgagtccgtg aggacgaaac gagtaagctc gtc                         43


<210>  5
<211>  68
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of hepatitis delta virus (HDV) ribozyme

<400>  5
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg       60

aatgggac                                                                68


<210>  6
<211>  1275
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pTRV2  CP- 2b with the QQR-sgRNA

<400>  6
ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat       60

gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc      120

ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac      180

cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt      240

aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac      300

gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg      360

ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt      420

tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg      480

tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg      540

aatcacttgc gctaatcaac aagcttaaaa gcttgttaac gaggtaattc ttcccctcag      600

gaggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa      660

agtggcaccg agtcggtgct ttttttctcg agggatttaa ggacgtgaac tctgttgaga      720

tctctgtgaa attcagaggg tgggtgatac catattcact gatgccatta gcgacatcta      780

aatagggcta attgtgacta atttgaggga atttccttta ccattgacgt cagtgtcgtt      840

ggtagcattt gagtttcgaa ttctctagaa ggcctccatg gggatccggt accgagctca      900

cgcgtctcga ggcccgggca tgtcccgaag acattaaact acggttcttt aagtagatcc      960

gtgtctgaag ttttaggttc aatttaaacc tacgagattg acattctcga ctgatcttga     1020

ttgatcggta agtcttttgt aatttaattt tctttttgat tttattttaa attgttatct     1080

gtttctgtgt atagactgtt tgagatcggc gtttggccga ctcattgtct taccataggg     1140

gaacggactt tgtttgtgtt gttattttat ttgtatttta ttaaaattct caacgatctg     1200

aaaaagcctc gcggctaaga gattgttggg gggtgagtaa gtacttttaa agtgatgatg     1260

gttacaaagg caaaa                                                      1275


<210>  7
<211>  2546
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pTRV2  CP-nptII(k)-sgRNA:  2b:DsRed

<400>  7
ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca       60

agacccttcc tctatataag gaagttcatt tcatttggag aggatccggc gcgccataaa      120

acattgcacc tatggtgttg ccctggctgg ggtatgtcag tgatcgcagt agaatgtact      180

aattgacaag ttggagaata cggtagaacg tccttatcca acacagcctt tatccctctc      240

cctgacgagg tttttgtcag tgtaatattt ctttttgaac tatccagctt agtaccgtac      300

gggaaagtga ctggtgtgct tatctttgaa atgttacttt gggtttcggt tctttaggtt      360

agtaagaaag cacttgtctt ctcatacaaa ggaaaacctg agacgtatcg cttacgaaag      420

tagcaatgaa agaaaggtgg tggttttaat cgctaccgca aaaacgatgg ggtcgtttta      480

attaacttct cctacgcaag cgtctaaacg gacgttgggg ttttgctagt ttctttagag      540

aaaactagct aagtctttaa tgttatcatt agagatggca taaatataat acttgtgtct      600

gctgataaga tcattttaat ttggacgatt agacttgttg aactacaggt tactgaatca      660

cttgcgctaa tcaacaagct tgttaacgag ggccatctga tgagtccgtg aggacgaaac      720

gagtaagctc gtcgctgtca gcgcaggggc gccgttttag agctagaaat agcaagttaa      780

aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggcc      840

ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg      900

ggacctcgag gtcgacagct ggatttaagg acgtgaactc tgttgagatc tctgtgaaat      960

tcagagggtg ggtgatacca tattcactga tgccattagc gacatctaaa tagggctaat     1020

tgtgactaat ttgagggaat ttcctttacc attgacgtca gtgtcgttgg tagcatttga     1080

gtttcgtaac tataacggtc ctaaggtagc gaacatcttg ttctggggtt tcacactatc     1140

tttagagaaa gtgttaagtt aattaagtta tcttaattaa gagcataatt atactgattt     1200

gtctctcgtt gatagagtct atcattctgt tactaaaaat ttgacaactc ggtttgctga     1260

cctactggtt actgtatcac ttacccgagt taacgagatg gcctcctccg agaacgtcat     1320

caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga     1380

gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt     1440

gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg     1500

ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc     1560

cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac     1620

ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa     1680

cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga     1740

gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa     1800

ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca     1860

gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta     1920

caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc tgtgagtcga     1980

ccccgaagac attaaactac ggttctttaa gtagatccgt gtctgaagtt taggttcaat     2040

ttaaacctac gagattgaca ttctcgactg atcttgattg atcggtaagt cttttgtaat     2100

ttaattttct ttttgatttt attttaaatt gttatctgtt tctgtgtata gactgtttga     2160

gatcggcgtt tggccgactc attgtcttac cataggggaa cggactttgt ttgtgttgtt     2220

attttatttg tattttatta aaattctcaa cgatctgaaa aagcctcgcg gctaagagat     2280

tgttgggggg tgagtaagta cttttaaagt gatgatggtt acaaaggcaa aaggggtaaa     2340

acccctcgcc tacgtaagcg ttattacgcc cgtctgtact tatatcagta cactgacgag     2400

tccctaaagg acgaaacggg aggaattctg caggagtggg gaggcacgat ggccgctttg     2460

gtcgatcgac gggatcgatc ctgctttaat gagatatgcg agacgcctat gatcgcatga     2520

tatttgcttt caattctgtt gtgcac                                          2546


<210>  8
<211>  2546
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pTRV2  CP-PDS-sgRNA:  2b:DsRed

<400>  8
ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca       60

agacccttcc tctatataag gaagttcatt tcatttggag aggatccggc gcgccataaa      120

acattgcacc tatggtgttg ccctggctgg ggtatgtcag tgatcgcagt agaatgtact      180

aattgacaag ttggagaata cggtagaacg tccttatcca acacagcctt tatccctctc      240

cctgacgagg tttttgtcag tgtaatattt ctttttgaac tatccagctt agtaccgtac      300

gggaaagtga ctggtgtgct tatctttgaa atgttacttt gggtttcggt tctttaggtt      360

agtaagaaag cacttgtctt ctcatacaaa ggaaaacctg agacgtatcg cttacgaaag      420

tagcaatgaa agaaaggtgg tggttttaat cgctaccgca aaaacgatgg ggtcgtttta      480

attaacttct cctacgcaag cgtctaaacg gacgttgggg ttttgctagt ttctttagag      540

aaaactagct aagtctttaa tgttatcatt agagatggca taaatataat acttgtgtct      600

gctgataaga tcattttaat ttggacgatt agacttgttg aactacaggt tactgaatca      660

cttgcgctaa tcaacaagct tgttaacgag ggccatctga tgagtccgtg aggacgaaac      720

gagtaagctc gtcgccgtta atttgagagt ccagttttag agctagaaat agcaagttaa      780

aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggcc      840

ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg      900

ggacctcgag gtcgacagct ggatttaagg acgtgaactc tgttgagatc tctgtgaaat      960

tcagagggtg ggtgatacca tattcactga tgccattagc gacatctaaa tagggctaat     1020

tgtgactaat ttgagggaat ttcctttacc attgacgtca gtgtcgttgg tagcatttga     1080

gtttcgtaac tataacggtc ctaaggtagc gaacatcttg ttctggggtt tcacactatc     1140

tttagagaaa gtgttaagtt aattaagtta tcttaattaa gagcataatt atactgattt     1200

gtctctcgtt gatagagtct atcattctgt tactaaaaat ttgacaactc ggtttgctga     1260

cctactggtt actgtatcac ttacccgagt taacgagatg gcctcctccg agaacgtcat     1320

caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga     1380

gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt     1440

gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg     1500

ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc     1560

cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac     1620

ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa     1680

cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga     1740

gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa     1800

ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca     1860

gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta     1920

caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc tgtgagtcga     1980

ccccgaagac attaaactac ggttctttaa gtagatccgt gtctgaagtt taggttcaat     2040

ttaaacctac gagattgaca ttctcgactg atcttgattg atcggtaagt cttttgtaat     2100

ttaattttct ttttgatttt attttaaatt gttatctgtt tctgtgtata gactgtttga     2160

gatcggcgtt tggccgactc attgtcttac cataggggaa cggactttgt ttgtgttgtt     2220

attttatttg tattttatta aaattctcaa cgatctgaaa aagcctcgcg gctaagagat     2280

tgttgggggg tgagtaagta cttttaaagt gatgatggtt acaaaggcaa aaggggtaaa     2340

acccctcgcc tacgtaagcg ttattacgcc cgtctgtact tatatcagta cactgacgag     2400

tccctaaagg acgaaacggg aggaattctg caggagtggg gaggcacgat ggccgctttg     2460

gtcgatcgac gggatcgatc ctgctttaat gagatatgcg agacgcctat gatcgcatga     2520

tatttgcttt caattctgtt gtgcac                                          2546


<210>  9
<211>  4104
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of Cas9

<400>  9
atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agac                                            4104


<210>  10
<211>  1368
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cas9 AA sequence

<400>  10

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  11
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  11
ctatccttcg caagaccctt cc                                                22


<210>  12
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  12
gtctgccagt tcagttcgtt gttc                                              24


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  13
agacaatcgg ctgctctgat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  14
ggccattttc caccatgata                                                   20


<210>  15
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  15
gctttgcttg agaaaagctc tc                                                22


<210>  16
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  16
acataacaaa ttcctttgca agc                                               23


<210>  17
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  17
ctatccttcg caagaccctt cc                                                22


<210>  18
<211>  2147
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pTRV  CP  2b QQR-sgRNA 2spP-DsRed

<400>  18
cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta gaatgtacta       60

attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt atccctctcc      120

ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta gtaccgtacg      180

ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt ctttaggtta      240

gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc ttacgaaagt      300

agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg gtcgttttaa      360

ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt tctttagaga      420

aaactagcta agtctttaat gttatcatta gagatggcat aaatataata cttgtgtctg      480

ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt actgaatcac      540

ttgcgctaat caacaagctt aaaagcttgt taacgaggta attcttcccc tcaggaggtt      600

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      660

accgagtcgg tgcttttttt ctcgagggat ttaaggacgt gaactctgtt gagatctctg      720

tgaaattcag agggtgggtg ataccatatt cactgatgcc attagcgaca tctaaatagg      780

gctaattgtg actaatttga gggaatttcc tttaccattg acgtcagtgt cgttggtagc      840

atttgagttt cgtaactata acggtcctaa ggtagcgaac atcttgttct ggggtttcac      900

actatcttta gagaaagtgt taagttaatt aagttatctt aattaagagc ataattatac      960

tgatttgtct ctcgttgata gagtctatca ttctgttact aaaaatttga caactcggtt     1020

tgctgaccta ctggttactg tatcacttac ccgagttaac gagatggcct cctccgagaa     1080

cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga     1140

gttcgagatc gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct     1200

gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca     1260

gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc     1320

cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac     1380

cgtgacccag gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg     1440

cgtgaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc     1500

caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa     1560

gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc     1620

cgtgcagctg cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga     1680

ggactacacc atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgtg     1740

agtcgaccgg gcatgtcccg aagacattaa actacggttc tttaagtaga tccgtgtctg     1800

aagttttagg ttcaatttaa acctacgaga ttgacattct cgactgatct tgattgatcg     1860

gtaagtcttt tgtaatttaa ttttcttttt gattttattt taaattgtta tctgtttctg     1920

tgtatagact gtttgagatc ggcgtttggc cgactcattg tcttaccata ggggaacgga     1980

ctttgtttgt gttgttattt tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc     2040

ctcgcggcta agagattgtt ggggggtgag taagtacttt taaagtgatg atggttacaa     2100

aggcaaaagg ggtaaaaccc ctcgcctacg taagcgttat tacgccc                   2147


<210>  19
<211>  14400
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pK7WGF2-hCas9

<400>  19
atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtga     4140

agggtgggcg cgccgaccca gctttcttgt acaaagtggt gatatcccgc ggccatgcta     4200

gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga     4260

ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt     4320

gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa     4380

aatttctaat tcctaaaacc aaaatccagt gacctgcagg catgcgacgt cgggccctct     4440

agaggatccc cgggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt     4500

atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa     4560

cgttgaagga gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca     4620

gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct     4680

tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat     4740

attcactctc aactcgatcg aggcatgatt gaacaagatg gattgcacgc aggttctccg     4800

gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct     4860

gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac     4920

ctgtccggtg ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg     4980

acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg     5040

ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa     5100

gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca     5160

ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt     5220

gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc     5280

aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc     5340

ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg     5400

ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt     5460

ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag     5520

cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggac     5580

tctagctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat     5640

cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta     5700

ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg     5760

caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta     5820

tcgcgcgcgg tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc     5880

ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa     5940

tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct     6000

cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag     6060

agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac     6120

taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga     6180

tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctcgt     6240

tcggttcgta agctgtaatg caagtagcgt aactgccgtc acgcaactgg tccagaacct     6300

tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttct tgttatgaca     6360

tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg     6420

ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa     6480

acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag     6540

gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc     6600

tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc     6660

gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct     6720

tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac     6780

atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat     6840

gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg     6900

acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat     6960

ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg     7020

ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac     7080

agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc     7140

ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa     7200

gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc     7260

gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg     7320

cttcgccggc gttaactcaa gcgattagat gcactaagca cataattgct cacagccaaa     7380

ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt     7440

gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     7500

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     7560

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     7620

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct     7680

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     7740

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     7800

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     7860

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     7920

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     7980

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     8040

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     8100

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     8160

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat     8220

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc     8280

agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg     8340

tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag     8400

ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca     8460

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     8520

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     8580

aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg     8640

ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg     8700

ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag     8760

ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt     8820

aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac     8880

ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt     8940

cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg     9000

acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc     9060

ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc     9120

cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt     9180

gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa     9240

ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat     9300

gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc     9360

tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt     9420

ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag     9480

gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat     9540

gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag     9600

tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta     9660

tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac     9720

actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg     9780

gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat     9840

gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc     9900

gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc     9960

tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat    10020

gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc    10080

cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca cgccgattcc tcgggcttgg    10140

gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc    10200

ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca    10260

tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct    10320

ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg    10380

tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct    10440

ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg    10500

gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt    10560

ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg    10620

attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac    10680

gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc    10740

gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct    10800

caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg    10860

gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg    10920

cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt    10980

caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc    11040

aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt    11100

gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa    11160

gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat    11220

atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg    11280

gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga    11340

caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac    11400

gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg    11460

gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg    11520

gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc    11580

gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt    11640

gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc    11700

tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg    11760

cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac    11820

acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta    11880

cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg    11940

cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc    12000

atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg    12060

gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg    12120

acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg    12180

ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa    12240

aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa    12300

gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa    12360

gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat    12420

aaaccttttc acgccctttt aaatatccgt tattctaata aacgctcttt tctcttaggt    12480

ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa    12540

tctgatccaa gctcaagcta agcttgagct ctcccatatg gtcgactaga gccaagctga    12600

tctcctttgc cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa    12660

agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct    12720

tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctca    12780

tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta    12840

aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc    12900

tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc    12960

aagtaataga gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt    13020

caaaaattca gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac    13080

agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca    13140

ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga    13200

cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc    13260

acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata    13320

aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac    13380

ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt    13440

gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc    13500

cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat    13560

accacaacaa aacaaacaac aaacaacatt acaatttact attctagtcg acctgcaggc    13620

ggccgcacta gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg    13680

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc    13740

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg    13800

ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc    13860

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag    13920

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag    13980

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac    14040

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac    14100

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc    14160

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg    14220

cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc    14280

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag    14340

ctgtacaagg atatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc    14400


<210>  20
<211>  2756
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of pTRV  2b ribo-DsRed-ribo

<400>  20
gatccggcgc gccataaaac attgcaccta tggtgttgcc ctggctgggg tatgtcagtg       60

atcgcagtag aatgtactaa ttgacaagtt ggagaatacg gtagaacgtc cttatccaac      120

acagccttta tccctctccc tgacgaggtt tttgtcagtg taatatttct ttttgaacta      180

tccagcttag taccgtacgg gaaagtgact ggtgtgctta tctttgaaat gttactttgg      240

gtttcggttc tttaggttag taagaaagca cttgtcttct catacaaagg aaaacctgag      300

acgtatcgct tacgaaagta gcaatgaaag aaaggtggtg gttttaatcg ctaccgcaaa      360

aacgatgggg tcgttttaat taacttctcc tacgcaagcg tctaaacgga cgttggggtt      420

ttgctagttt ctttagagaa aactagctaa gtctttaatg ttatcattag agatggcata      480

aatataatac ttgtgtctgc tgataagatc attttaattt ggacgattag acttgttgaa      540

ctacaggtta ctgaatcact tgcgctaatc aacaagctta tgggagatat gtacgatgaa      600

tcatttgaca agtcgggcgg tcctgctgac ttgatggacg attcttgggt ggaatcagtt      660

tcgtggaaag atctgttgaa gaagttacac agcataaaat ttgcactaca gtctggtaga      720

gatgagatca ctgggttact agcggcactg aatagacagt gtccttattc accatatgag      780

cagtttccag ataagaaggt gtatttcctt ttagactcac gggctaacag tgctcttggt      840

gtgattcaga acgcttcagc gttcaagaga cgagctgatg agaagaatgc agtggcgggt      900

gttacaaata ttcctgcgaa tccaaacaca acggttacga cgaaccaagg gagtactact      960

actaccaagg cgaacactgg ctcgactttg gaagaagact tgtacactta ttacaaattc     1020

gatgatgcct ctacagcttt ccacaaatct ctaacttcgt tagagaacat ggagttgaag     1080

agttattacc gaaggaactt tgagaaagta ttcgggatta agtttggtgg agcagctgct     1140

agttcatctg caccgcctcc agcgagtgga ggtccgatac gtcctaatcc ctagggattt     1200

aaggacgtga actctgttga gatctctgtg aaattcagag ggtgggtgat accatattca     1260

ctgatgccat tagcgacatc taaatagggc taattgtgac taatttgagg gaatttcctt     1320

taccattgac gtcagtgtcg ttggtagcat ttgagtttcg taactataac ggtcctaagg     1380

tagcgaacat cttgttctgg ggtttcacac tatctttaga gaaagtgtta agttaattaa     1440

gttatcttaa ttaagagcat aattatactg atttgtctct cgttgataga gtctatcatt     1500

ctgttactaa aaatttgaca actcggtttg ctgacctact ggttactgta tcacttaccc     1560

gagttaacga gggccatctg atgagtccgt gaggacgaaa cgagtaagct cgtcatggcc     1620

tcctccgaga acgtcatcac cgagttcatg cgcttcaagg tgcgcatgga gggcaccgtg     1680

aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac     1740

accgtgaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc     1800

ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac     1860

aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc     1920

ggcgtggcga ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg     1980

aagttcatcg gcgtgaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc     2040

tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagacccac     2100

aaggccctga agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg     2160

gccaagaagc ccgtgcagct gcccggctac tactacgtgg acgccaagct ggacatcacc     2220

tcccacaacg aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac     2280

ctgttcctgt gaggccggca tggtcccagc ctcctcgctg gcgccggctg ggcaacatgc     2340

ttcggcatgg cgaatgggac gtcgaccccg aagacattaa actacggttc tttaagtaga     2400

tccgtgtctg aagtttaggt tcaatttaaa cctacgagat tgacattctc gactgatctt     2460

gattgatcgg taagtctttt gtaatttaat tttctttttg attttatttt aaattgttat     2520

ctgtttctgt gtatagactg tttgagatcg gcgtttggcc gactcattgt cttaccatag     2580

gggaacggac tttgtttgtg ttgttatttt atttgtattt tattaaaatt ctcaacgatc     2640

tgaaaaagcc tcgcggctaa gagattgttg gggggtgagt aagtactttt aaagtgatga     2700

tggttacaaa ggcaaaaggg gtaaaacccc tcgcctacgt aagcgttatt acgccc         2756


<210>  21
<211>  214
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of RGR1RGR1

<400>  21
ggccatctga tgagtccgtg aggacgaaac gagtaagctc gtcgctgtca gcgcaggggc       60

gccgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa      120

agtggcaccg agtcggtgct ttttttggcc ggcatggtcc cagcctcctc gctggcgccg      180

gctgggcaac atgcttcggc atggcgaatg ggac                                  214


<210>  22
<211>  214
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of RGR2

<400>  22
ggccatctga tgagtccgtg aggacgaaac gagtaagctc gtcgccgtta atttgagagt       60

ccagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa      120

agtggcaccg agtcggtgct ttttttggcc ggcatggtcc cagcctcctc gctggcgccg      180

gctgggcaac atgcttcggc atggcgaatg ggac                                  214


<210>  23
<211>  202
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of CP subgenomic promoter

<400>  23
cgttttaatt aacttctcct acgcaagcgt ctaaacggac gttggggttt tgctagtttc       60

tttagagaaa actagctaag tctttaatgt tatcattaga gatggcataa atataatact      120

tgtgtctgct gataagatca ttttaatttg gacgattaga cttgttgaac tacaggttac      180

tgaatcactt gcgctaatca ac                                               202


<210>  24
<211>  253
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of NOS terminator

<400>  24
agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg       60

aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt      120

tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc      180

gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattaaac      240

tatcagtgtt tga                                                         253


<210>  25
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of omega enhancer

<400>  25
gtatttttac aacaattacc aacaacaaca aacaacagac aacattacaa ttactattta       60

caattacaat                                                              70


<210>  26
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of qqr

<400>  26
ttcttcccct cctgagggga agaa                                              24


<210>  27
<211>  1065
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of full length 2b

<400>  27
atgcacgaat tacttaggaa gtggcttgac gacactaatg tgttattgtt agataatggt       60

ttggtggtca aggtacgtag tagagtccca catattcgca cgtatgaagt aattggaaag      120

ttgtcagttt ttgataattc actgggagat gatacgctgt ttgagggaaa agtagagaac      180

gtatttgttt ttatgttcag gcggttcttg tgtgtcaaca aagatggaca ttgttactca      240

aggaagcacg atgagcttta ttattacgga cgagtggact tagattctgt gagtaaggtt      300

acctcagggt acgagaaact ctttattcac agagaacttt atatcttaac agatttaatt      360

gagagagtga gtaagttctt taacttagct caggatgtgg tagaagcaag ttttgagtat      420

gccaaggttg aagagaggtt aggtcacgtc agaaacgtgt tgcaactggc gggtggaaaa      480

tccacgaatg ccgatttgac aattaagatt tctgacgatg tcgaacaact gcttggaaaa      540

cgtggtggat tcttgaaggt tgtgaacggt atcttgagca agaatggtag tgacgtagtc      600

actaacgaca atgagcttat tcatgcaatt aaccaaaatc tggtaccaga taaagtcatg      660

tctgtgtcga acgtaatgaa agagactggg tttctgcagt ttccaaagtt tttatctaag      720

ttggaaggac aggtaccgaa aggaacaaaa tttctagaca aacacgttcc tgattttact      780

tggatacaag ctcttgaaga aagagtgaat attcggagag gagaatcggg acttcagact      840

ctattagctg atatcgttcc gaggaatgct attgctgctc agaaattgac aatgctaggt      900

tacatcgagt atcacgacta tgtggtgatc gtctgtcagt ctggagtatt tagtgacgat      960

tgggcgacat gtagaatgct ttgggcagca ctatctagtg ctcaactata tacctatgtt     1020

gacgccagta gaatcggtcc aatcgtttac ggttggttat tgtga                     1065


<210>  28
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  28
gtctgccagt tcagttcgtt gttc                                              24


<210>  29
<211>  73
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII

<400>  29
atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc       60

tgtccggtgc cct                                                          73


<210>  30
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -8bp indel.

<400>  30
atgccgccgt gttccggctg tcagcgcccg gttctttttg tcaagaccga cctgtccggt       60

gccct                                                                   65


<210>  31
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -8bp indel.

<400>  31
atgccgccgt gttccggctg tcagcgcacg gttctttttg tcaagaccga cctgtccggt       60

gccct                                                                   65


<210>  32
<211>  71
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -2bp indel.

<400>  32
atgccgccgt gttccggctg tcagcgcagg ggcccggttc tttttgtcaa gaccgacctg       60

tccggtgccc t                                                            71


<210>  33
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  33
atgccgccgt gttccggctg tcagcgcagg ggctgcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  34
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  34
atgccgccgt gttccggctg tcagcgcagg ggctgcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  35
<211>  63
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -10bp indel.

<400>  35
atgccgccgt gttccggctg tccgcccggt tctttttgtc aagaccgacc tgtccggtgc       60

cct                                                                     63


<210>  36
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  36
atgccgccgt gttccggctg tcagcgcagg ggctgcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  37
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  37
atgccgccgt gttccggctg tcagcgcagg ggcagcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  38
<211>  72
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -1bp indel.

<400>  38
atgccgccgt gttccggctg tcagcgcagg gggcccggtt ctttttgtca agaccgacct       60

gtccggtgcc ct                                                           72


<210>  39
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  39
atgccgccgt gttccggctg tcagcgcagg ggcagcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  40
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with +1bp indel.

<400>  40
atgccgccgt gttccggctg tcagcgcagg ggccgcccgg ttctttttgt caagaccgac       60

ctgtccggtg ccct                                                         74


<210>  41
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes NptII with -8bp indel.

<400>  41
atgccgccgt gttccggctg tcagcgcccg gttctttttg tcaagaccga cctgtccggt       60

gccct                                                                   65


<210>  42
<211>  73
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes PDS

<400>  42
taaaatgccc caaattggac ttgtttctgc cgttaatttg agagtccaag gtaattcagc       60

ttatctttgg agc                                                          73


<210>  43
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes PDS with +1bp indel.

<400>  43
taaaatgccc caaattggac ttgtttctgc cgttaatttg agagttccaa ggtaattcag       60

cttatctttg gagc                                                         74


<210>  44
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes PDS with +1bp indel.

<400>  44
taaaatgccc caaattggac ttgtttctgc cgttaatttg agagttccaa ggtaattcag       60

cttatctttg gagc                                                         74


<210>  45
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target genes PDS with -3bp indel.

<400>  45
taaaatgccc caaattggac ttgtttctgc cgttaatttg agccaaggta attcagctta       60

tctttggagc                                                              70


<210>  46
<211>  73
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of  a fragment of the mGUS gene, targeted by 
       the pTRV2 -QQR-sgRNA

<400>  46
ctgcagtcga cggtaccatg ttcttcccct cctgagggga agaattacgt cctgtagaaa       60

ccccaacccg tga                                                          73


<210>  47
<211>  69
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of  a fragment of the mGUS gene, targeted by 
       the pTRV2 -QQR-sgRNA  with -4bp indel.

<400>  47
ctgcagtcga cggtaccatg ttcttcccct cggggaagaa ttacgtcctg tagaaacccc       60

aacccgtga                                                               69


<210>  48
<211>  73
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of  a fragment of the mGUS gene, targeted by 
       the pTRV2 -QQR-sgRNA  with +1bp indel.

<400>  48
ctgcagtcga cggtaccatg ttcttcccct ccagagggga agaattacgt cctgtagaaa       60

ccccaacccg tga                                                          73


<210>  49
<211>  1593
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV RNA2-CP-sgNptII-k- sgNptII-i

<400>  49
cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta gaatgtacta       60

attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt atccctctcc      120

ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta gtaccgtacg      180

ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt ctttaggtta      240

gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc ttacgaaagt      300

agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg gtcgttttaa      360

ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt tctttagaga      420

aaactagcta agtctttaat gttatcatta gagatggcat aaatataata cttgtgtctg      480

ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt actgaatcac      540

ttgcgctaat caacaagctt aaaagcttgt taacgaggct gtcagcgcag gggcgccgtt      600

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      660

accgagtcgg tgcttttttt ctcgagggat ttaaggacgt gaactctgtt gagatctctg      720

tgaaattcag agggtgggtg ataccatatt cactgatgcc attagcgaca tctaaatagg      780

gctaattgtg actaatttga gggaatttcc tttaccattg acgtcagtgt cgttggtagc      840

atttgagttt cgtaactata acggtcctaa ggtagcgaac atcttgttct ggggtttcac      900

actatcttta gagaaagtgt taagttaatt aagttatctt aattaagagc ataattatac      960

tgatttgtct ctcgttgata gagtctatca ttctgttact aaaaatttga caactcggtt     1020

tgctgaccta ctggttactg tatcacttac ccgagttaac gaggctcgac gttgtcactg     1080

aaggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa     1140

agtggcaccg agtcggtgct ttttttctcg agcccgggta gggataacag ggtaatgtcg     1200

accccgaaga cattaaacta cggttcttta agtagatccg tgtctgaagt tttaggttca     1260

atttaaacct acgagattga cattctcgac tgatcttgat tgatcggtaa gtcttttgta     1320

atttaatttt ctttttgatt ttattttaaa ttgttatctg tttctgtgta tagactgttt     1380

gagatcggcg tttggccgac tcattgtctt accatagggg aacggacttt gtttgtgttg     1440

ttattttatt tgtattttat taaaattctc aacgatctga aaaagcctcg cggctaagag     1500

attgttgggg ggtgagtaag tacttttaaa gtgatgatgg ttacaaaggc aaaaggggta     1560

aaacccctcg cctacgtaag cgttattacg ccc                                  1593


<210>  50
<211>  2308
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV RNA2-CP-sgNptII-i- sgNptII-kDsRed2

<400>  50
cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta gaatgtacta       60

attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt atccctctcc      120

ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta gtaccgtacg      180

ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt ctttaggtta      240

gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc ttacgaaagt      300

agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg gtcgttttaa      360

ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt tctttagaga      420

aaactagcta agtctttaat gttatcatta gagatggcat aaatataata cttgtgtctg      480

ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt actgaatcac      540

ttgcgctaat caacaagctg taaagcttgt taacgaggct cgacgttgtc actgaaggtt      600

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      660

accgagtcgg tgcttttttt ctcgagtttt tcagcaagat ctcgaggacg tcagtgtcgt      720

tggtagcatt tgagtttcgg ctgtcagcgc aggggcgccg ttttagagct agaaatagca      780

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      840

ttcctaagga tttaaggacg tgaactctgt tgagatctct gtgaaattca gagggtgggt      900

gataccatat tcactgatgc cattagcgac atctaaatag ggctaattgt gactaatttg      960

agggaatttc ctttaccatt gacgtcagtg tcgttggtag catttgagtt tcgtaactat     1020

aacggtccta aggtagcgaa catcttgttc tggggtttca cactatcttt agagaaagtg     1080

ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat     1140

agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact     1200

gtatcactta cccgagttaa cgagatggcc tcctccgaga acgtcatcac cgagttcatg     1260

cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag     1320

ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc     1380

cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac     1440

gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag     1500

tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc     1560

ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac     1620

ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc     1680

cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga cggcggccac     1740

tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac     1800

tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac catcgtggag     1860

cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt gagtcgaccg ggcatgtccc     1920

gaagacatta aactacggtt ctttaagtag atccgtgtct gaagttttag gttcaattta     1980

aacctacgag attgacattc tcgactgatc ttgattgatc ggtaagtctt ttgtaattta     2040

attttctttt tgattttatt ttaaattgtt atctgtttct gtgtatagac tgtttgagat     2100

cggcgtttgg ccgactcatt gtcttaccat aggggaacgg actttgtttg tgttgttatt     2160

ttatttgtat tttattaaaa ttctcaacga tctgaaaaag cctcgcggct aagagattgt     2220

tggggggtga gtaagtactt ttaaagtgat gatggttaca aaggcaaaag gggtaaaacc     2280

cctcgcctac gtaagcgtta ttacgccc                                        2308


<210>  51
<211>  2237
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV RNA2-CP-sgNptII-i- sgNptII-kDsRed2

<400>  51
cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta gaatgtacta       60

attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt atccctctcc      120

ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta gtaccgtacg      180

ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt ctttaggtta      240

gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc ttacgaaagt      300

agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg gtcgttttaa      360

ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt tctttagaga      420

aaactagcta agtctttaat gttatcatta gagatggcat aaatataata cttgtgtctg      480

ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt actgaatcac      540

ttgcgctaat caacaagctg taaagcttgt taacgaggct cgacgttgtc actgaaggtt      600

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      660

accgagtcgg tgcttttttt ctcgagggat ttaaggacgt gaactctgtt gagatctctg      720

tgaaattcag agggtgggtg ataccatatt cactgatgcc attagcgaca tctaaatagg      780

gctaattgtg actaatttga gggaatttcc tttaccattg acgtcagtgt cgttggtagc      840

atttgagttt cggctgtcag cgcaggggcg ccgttttaga gctagaaata gcaagttaaa      900

ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttcctaa      960

ggtagcgaac atcttgttct ggggtttcac actatcttta gagaaagtgt taagttaatt     1020

aagttatctt aattaagagc ataattatac tgatttgtct ctcgttgata gagtctatca     1080

ttctgttact aaaaatttga caactcggtt tgctgaccta ctggttactg tatcacttac     1140

ccgagttaac gagatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt     1200

gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg     1260

cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt     1320

cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc     1380

cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt     1440

gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg     1500

ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat     1560

gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt     1620

gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga     1680

gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga     1740

cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg     1800

caccgagggc cgccaccacc tgttcctgtg agtcgaccgg gcatgtcccg aagacattaa     1860

actacggttc tttaagtaga tccgtgtctg aagttttagg ttcaatttaa acctacgaga     1920

ttgacattct cgactgatct tgattgatcg gtaagtcttt tgtaatttaa ttttcttttt     1980

gattttattt taaattgtta tctgtttctg tgtatagact gtttgagatc ggcgtttggc     2040

cgactcattg tcttaccata ggggaacgga ctttgtttgt gttgttattt tatttgtatt     2100

ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta agagattgtt ggggggtgag     2160

taagtacttt taaagtgatg atggttacaa aggcaaaagg ggtaaaaccc ctcgcctacg     2220

taagcgttat tacgccc                                                    2237


<210>  52
<211>  2053
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV RNA2-CP-sgNptII-i- sgNptII-kDsRed2

<400>  52
cataaaacat tgcacctatg gtgttgccct ggctggggta tgtcagtgat cgcagtagaa       60

tgtactaatt gacaagttgg agaatacggt agaacgtcct tatccaacac agcctttatc      120

cctctccctg acgaggtttt tgtcagtgta atatttcttt ttgaactatc cagcttagta      180

ccgtacggga aagtgactgg tgtgcttatc tttgaaatgt tactttgggt ttcggttctt      240

taggttagta agaaagcact tgtcttctca tacaaaggaa aacctgagac gtatcgctta      300

cgaaagtagc aatgaaagaa aggtggtggt tttaatcgct accgcaaaaa cgatggggtc      360

gttttaatta acttctccta cgcaagcgtc taaacggacg ttggggtttt gctagtttct      420

ttagagaaaa ctagctaagt ctttaatgtt atcattagag atggcataaa tataatactt      480

gtgtctgctg ataagatcat tttaatttgg acgattagac ttgttgaact acaggttact      540

gaatcacttg cgctaatcaa caagctgtaa agcttgttaa cgaggctcga cgttgtcact      600

gaaggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa      660

aagtggcacc gagtcggtgc tttttttctc gagggattta aggacgtgaa ctctgttgag      720

atctctgtga aattcagagg gtgggtgata ccatattcac tgatgccatt agcgacatct      780

aaatagggct aattgtgact aatttgaggg aatttccttt accattgacg tcagtgtcgt      840

tggtagcatt tgagtttcgg ctgtcagcgc aggggcgccg ttttagagct agaaatagca      900

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      960

ttcctaagga tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt caaggtgcgc     1020

atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc     1080

tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct gcccttcgcc     1140

tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc     1200

gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg     1260

aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca ggacggctgc     1320

ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtgatgcag     1380

aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg     1440

aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc     1500

aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggacgcc     1560

aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcacc     1620

gagggccgcc accacctgtt cctgtgagtc gaccgggcat gtcccgaaga cattaaacta     1680

cggttcttta agtagatccg tgtctgaagt tttaggttca atttaaacct acgagattga     1740

cattctcgac tgatcttgat tgatcggtaa gtcttttgta atttaatttt ctttttgatt     1800

ttattttaaa ttgttatctg tttctgtgta tagactgttt gagatcggcg tttggccgac     1860

tcattgtctt accatagggg aacggacttt gtttgtgttg ttattttatt tgtattttat     1920

taaaattctc aacgatctga aaaagcctcg cggctaagag attgttgggg ggtgagtaag     1980

tacttttaaa gtgatgatgg ttacaaaggc aaaaggggta aaacccctcg cctacgtaag     2040

cgttattacg ccc                                                        2053


<210>  53
<211>  103
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of nptII(i)-sgRNA

<400>  53
gctcgacgtt gtcactgaag gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  54
<211>  5414
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of a T-DNA with mGUS and nptII cassettes

<400>  54
gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac       60

aatctgatcc aagctcaagc taagcttagg cctgtcgacg cccgggcggt accgcgatcg      120

ctcgcgacct gcaggcataa agccgtcagt gtccgcataa agaaccaccc ataataccca      180

taatagctgt ttgccaaccg gtcaacatgt ggagcacgac acacttgtct actccaaaaa      240

tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat      300

atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt      360

ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga      420

agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga      480

aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga      540

gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc      600

aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc      660

tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca      720

ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg      780

acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca      840

agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc      900

gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacgt cgagagttct      960

caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca     1020

gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg     1080

aacgatagag atctcgagct caagcttcga attctgcagt cgacggtacc atgttcttcc     1140

cctcctgagg ggaagaatta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg     1200

acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg     1260

aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg     1320

ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac     1380

cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca     1440

aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag     1500

ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca     1560

acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga     1620

aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc     1680

tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag     1740

actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac     1800

tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag     1860

tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag     1920

ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag     1980

tgaagggcca acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc     2040

atgaagatgc ggacttacgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg     2100

cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag     2160

agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg     2220

gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca     2280

gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga     2340

tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata     2400

cccgtccgca agtgcacggg aatatttcgc cactggcgga agcaacgcgt aaactcgacc     2460

cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc gataccatca     2520

gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc caaagcggcg     2580

atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag gagaaactgc     2640

atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg cactcaatgt     2700

acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat caccgcgtct     2760

ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat tttgcgacct     2820

cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc gaccgcaaac     2880

cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc ggtgaaaaac     2940

cgcagcaggg aggcaaacaa tgaggatcca cctgatctag agtccgcaaa aatcaccagt     3000

ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca     3060

gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct     3120

tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca     3180

aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca tcgctacctt     3240

aggaccgtta tagttaatta ccctgttatc cctattaatt aagagctcgc taccttaaga     3300

gaggatatcg gcgcgccacc gggctgaaag cgacgttgga tgttaacatc tacaaattgc     3360

cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt gaggaaactg     3420

gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt cacctacgat     3480

ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg cctgtaagat     3540

cctttttacc gacaactcat ccacattgat ggtaggcaga aagttaaagg attatcgcaa     3600

gtcaatactt gcccattcat tgatctattt aaaggtgtgg cctcaaggat aatcgccaaa     3660

ccattatatt tgcaatctac caatccggac tcagatctcg agctcaagct tcgaattctg     3720

cagtcgacat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga     3780

ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc     3840

ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga     3900

atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg     3960

cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc     4020

cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg     4080

atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga     4140

aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc     4200

tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca     4260

tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg     4320

tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct     4380

atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg     4440

accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc     4500

gccttcttga cgagttcttc tgaggatcca cctagtctag aactagtccc tagagtcctg     4560

ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg     4620

cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt     4680

ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt     4740

actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg     4800

aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt     4860

attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca     4920

taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg     4980

cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat     5040

tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg     5100

gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg     5160

gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg     5220

gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggagcg gccgcggcgc     5280

gcctctagaa tttaaatgga tcctacgtac tcgaggaatt caattcggcg ttaattcagt     5340

acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac     5400

aatatatcct gcca                                                       5414


<210>  55
<211>  525
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene

<400>  55
agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc       60

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc      120

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag      180

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc      240

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg      300

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc      360

ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc      420

cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga      480

cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggcc                      525


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nptII 80F primer

<400>  56
agacaatcgg ctgctctgat                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nptII 604R primer

<400>  57
ggccattttc caccatgata                                                   20


<210>  58
<211>  390
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene

<400>  58
agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc       60

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc      120

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag      180

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc      240

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg      300

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc      360

ggatggaagc cggtcttgtc gatcaggatg                                       390


<210>  59
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nptII 470R primer

<400>  59
tcatcctgat cgacaagacc                                                   20


<210>  60
<211>  264
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene with 126bp deletion

<400>  60
agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg caagcgggaa       60

gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc      120

ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg      180

ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg      240

aagccggtct tgtcgatcag gatg                                             264


<210>  61
<211>  252
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene with 138bp deletion

<400>  61
agacaatcgg ctgctctgat gccgccgtgt tccggctgta agcgggaagg gactggctgc       60

tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag      120

tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat      180

tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg      240

tcgatcagga tg                                                          252


<210>  62
<211>  251
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene with 139bp deletion

<400>  62
gacaatcggc tgctctgatg ccgccgtgtt ccggctgtaa gcgggaaggg actggctgct       60

attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt      120

atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt      180

cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt      240

cgatcaggat g                                                           251


<210>  63
<211>  245
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene with 145bp deletion

<400>  63
agacaatcgg ctgctctgat gccgccgtgt tcaagcggga agggactggc tgctattggg       60

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat      120

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca      180

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca      240

ggatg                                                                  245


<210>  64
<211>  240
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of nptII gene with 150bp deletion

<400>  64
agacaatcgg ctgctctgat gccgccgaag cgggaaggga ctggctgcta ttgggcgaag       60

tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg      120

ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag      180

cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg      240


<210>  65
<211>  116
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of mGUS gene with 4bp deletion

<400>  65
accatttacg aacgatagag atctcgagct caagcttcga attctgcagt cgacggtacc       60

atgttcttcc cctcggggaa gaattacgtc ctgtagaaac cccaacccgt gaaatc          116


<210>  66
<211>  109
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Partial nucleotide sequence of mGUS gene with 11bp deletion

<400>  66
accatttacg aacgatagag atctcgagct caagcttcga attctgcagt cgacggtacc       60

atgttcttcc cctcctttac gtcctgtaga aaccccaacc cgtgaaatc                  109


<210>  67
<211>  2147
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV RNA2-CP-sgNptII-k2sgP-DsRed2

<400>  67
cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta gaatgtacta       60

attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt atccctctcc      120

ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta gtaccgtacg      180

ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt ctttaggtta      240

gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc ttacgaaagt      300

agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg gtcgttttaa      360

ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt tctttagaga      420

aaactagcta agtctttaat gttatcatta gagatggcat aaatataata cttgtgtctg      480

ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt actgaatcac      540

ttgcgctaat caacaagctt aaaagcttgt taacgaggct gtcagcgcag gggcgccgtt      600

ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc      660

accgagtcgg tgcttttttt ctcgagggat ttaaggacgt gaactctgtt gagatctctg      720

tgaaattcag agggtgggtg ataccatatt cactgatgcc attagcgaca tctaaatagg      780

gctaattgtg actaatttga gggaatttcc tttaccattg acgtcagtgt cgttggtagc      840

atttgagttt cgtaactata acggtcctaa ggtagcgaac atcttgttct ggggtttcac      900

actatcttta gagaaagtgt taagttaatt aagttatctt aattaagagc ataattatac      960

tgatttgtct ctcgttgata gagtctatca ttctgttact aaaaatttga caactcggtt     1020

tgctgaccta ctggttactg tatcacttac ccgagttaac gagatggcct cctccgagaa     1080

cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga     1140

gttcgagatc gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct     1200

gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca     1260

gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc     1320

cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac     1380

cgtgacccag gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg     1440

cgtgaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc     1500

caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa     1560

gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc     1620

cgtgcagctg cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga     1680

ggactacacc atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgtg     1740

agtcgaccgg gcatgtcccg aagacattaa actacggttc tttaagtaga tccgtgtctg     1800

aagttttagg ttcaatttaa acctacgaga ttgacattct cgactgatct tgattgatcg     1860

gtaagtcttt tgtaatttaa ttttcttttt gattttattt taaattgtta tctgtttctg     1920

tgtatagact gtttgagatc ggcgtttggc cgactcattg tcttaccata ggggaacgga     1980

ctttgtttgt gttgttattt tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc     2040

ctcgcggcta agagattgtt ggggggtgag taagtacttt taaagtgatg atggttacaa     2100

aggcaaaagg ggtaaaaccc ctcgcctacg taagcgttat tacgccc                   2147


<210>  68
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 3E,3F,3G

<400>  68
ttcttcccct caggggaaga a                                                 21


<210>  69
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 5C

<400>  69
ttcttcccct ctgaggggaa gaa                                               23


<210>  70
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 5F

<400>  70
ttcttcccct ctgaggggaa gaa                                               23


<210>  71
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 5H

<400>  71
ttcttcccct ctgaggggaa gaa                                               23


<210>  72
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 6A

<400>  72
ttcttcccct cggggaagaa                                                   20


<210>  73
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 7B

<400>  73
ttcttcccct ctctgagggg aagaa                                             25


<210>  74
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutated QQR target sequence in Tobacco lines 8A

<400>  74
ttcttcccct ccgt                                                         14


<210>  75
<211>  2314
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TRV with two guide RNA for the endogenic 
       TOM3 and TOM1 (Capital letters) petunia genes

<400>  75
ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat       60

gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc      120

ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac      180

cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt      240

aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac      300

gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg      360

ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt      420

tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg      480

tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg      540

aatcacttgc gctaatcaac aagcttgtta acgagtagtt gtgatgccta acctcgtttt      600

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      660

cgagtcggtg ctttttttct cgagggattt aaggacgtga actctgttga gatcttctag      720

aaagattaaa agcttgttaa cgagagtgtt tatcttccat cctagtttta gagctagaaa      780

tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc      840

tttttttctc gagggattta aggacgtgaa ctctgttgag atctctgtga aattcagagg      900

gtgggtgata ccatattcac tgatgccatt agcgacatct aaatagggct aattgtgact      960

aatttgaggg aatttccttt accattgacg tcagtgtcgt tggtagcatt tgagtttcgt     1020

aactataacg gtcctaaggt agcgaacatc ttgttctggg gtttcacact atctttagag     1080

aaagtgttaa gttaattaag ttatcttaat taagagcata attatactga tttgtctctc     1140

gttgatagag tctatcattc tgttactaaa aatttgacaa ctcggtttgc tgacctactg     1200

gttactgtat cacttacccg agttaacgag atggcctcct ccgagaacgt catcaccgag     1260

ttcatgcgct tcaaggtgcg catggagggc accgtgaacg gccacgagtt cgagatcgag     1320

ggcgagggcg agggccgccc ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag     1380

ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag     1440

gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc     1500

ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggcgaccgt gacccaggac     1560

tcctccctgc aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc     1620

tccgacggcc ccgtgatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg     1680

tacccccgcg acggcgtgct gaagggcgag acccacaagg ccctgaagct gaaggacggc     1740

ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc     1800

ggctactact acgtggacgc caagctggac atcacctccc acaacgagga ctacaccatc     1860

gtggagcagt acgagcgcac cgagggccgc caccacctgt tcctgtgagt cgaccgggca     1920

tgtcccgaag acattaaact acggttcttt aagtagatcc gtgtctgaag ttttaggttc     1980

aatttaaacc tacgagattg acattctcga ctgatcttga ttgatcggta agtcttttgt     2040

aatttaattt tctttttgat tttattttaa attgttatct gtttctgtgt atagactgtt     2100

tgagatcggc gtttggccga ctcattgtct taccataggg gaacggactt tgtttgtgtt     2160

gttattttat ttgtatttta ttaaaattct caacgatctg aaaaagcctc gcggctaaga     2220

gattgttggg gggtgagtaa gtacttttaa agtgatgatg gttacaaagg caaaaggggt     2280

aaaacccctc gcctacgtaa gcgttattac gccc                                 2314


<210>  76
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM1 Forward 110F primer

<400>  76
tcaactaata aggatcgagt tgagg                                             25


<210>  77
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM1 Reverse 783R primer

<400>  77
aacatggcca gacattattg c                                                 21


<210>  78
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM3 Forward 623F primer

<400>  78
tatttgccca cccacttgtt                                                   20


<210>  79
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM3 Reverse 411R primer

<400>  79
cgtgcctggt agtatatctc agc                                               23


<210>  80
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TOM1 target site

<400>  80
agtgtttatc ttccatccta                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of TOM3 target site

<400>  81
tagttgtgat gcctaacctc                                                   20


<210>  82
<211>  55
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM1 target site - 1base deletion in plant 3769-4

<400>  82
aattgtcttt ggatttcaca aacaagtgtt tatcttccat ctaaggtatg ttctt            55


<210>  83
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM1 target site - 1base insertion found in plant 3769-27

<400>  83
aattgtcttt ggatttcaca aacaagtgtt tatcttccat cactaaggta tgttctt          57


<210>  84
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM1 target site  10 base deletion found in plant 3769-41

<400>  84
aattgtcttt ggatttcaca aacaagtgtt tataaggtat gttctt                      46


<210>  85
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM1 target site  11 base deletion found in plant 3769-43

<400>  85
aattgtcttt ggatttcaca aacaagtgtt tataggtatg ttctt                       45


<210>  86
<211>  59
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  7 base deletion found in plant 3742-66

<400>  86
cgtcgggatg ttcagaagtt gcatcctgac atcacaacta tcttgatgtc cggttgtca        59


<210>  87
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  1 base deletion found in plant 3769-4

<400>  87
cgtcgggatg ttcagaagtt gcatcctgag ttaggcatca caactatctt gatgtccggt       60

tgtca                                                                   65


<210>  88
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  1 base insertion found in plant 3769-9

<400>  88
cgtcgggatg ttcagaagtt gcatcctgag agttaggcat cacaactatc ttgatgtccg       60

gttgtca                                                                 67


<210>  89
<211>  63
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  3 base deletion found in plant 3769-1

<400>  89
cgtcgggatg ttcagaagtt gcatcctgag aggcatcaca actatcttga tgtccggttg       60

tca                                                                     63


<210>  90
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  6 base deletion found in plant 3769-3

<400>  90
cgtcgggatg ttcagaagtt gcatcctgag catcacaact atcttgatgt ccggttgtca       60


<210>  91
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  8 base deletion and 2 base insertion 
       found in plant 3769-6

<400>  91
cgtcgggatg ttcagaagtt gcatcctgag cgtcacgact atcttgatgt ccggttgtca       60


<210>  92
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM3 target site  1 base deletion found in plant 3769-9

<400>  92
cgtcgggatg ttcagaagtt gcatcctgag agttaggcat cacaactatc ttgatgtccg       60

gttgtca                                                                 67


<210>  93
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  mutate TOM1 target site - 1base insertion found in plant 3769-27

<400>  93
aattgtcttt ggatttcaca aacaagtgtt tatcttccat ctctaaggta tgttctt          57


<210>  94
<211>  825
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM1 Petunia hybrida Coding sequence (taken from literature).

<400>  94
atgagtatga ccaactggtg ggatgagatc gatgaatcag ttgattggca agatgggatt       60

ttctactctc tttgtgcttc ttatgctctt gtctcagctg ttgcccttat tcaactaata      120

aggatcgagt tgagggtacc cgagtatggt tggacaacac aaaaggtttt ccatctgatg      180

aactttattg taaatggagt tcgtgcaatt gtctttggat ttcacaaaca agtgtttatc      240

ttccatccta aggttcttac tctgacaata ttggacctac cagggctcct tttcttctca      300

acatttacac tccttgttct attctgggct gaaatatatc accaggctag gagtttacca      360

acagataagc tcagaatttc ctatatttcg ataaatgctg caatgtactt cattcaggcc      420

tgtatctggg tttacatctg gctcaacgac aatagcactg tggaattcat tgggaagata      480

tttatagcag ttgtatcctt tattgcagcg ttgggctttc tgctctatgg cggaaggtta      540

tttctcatgc tgcggcgctt tcctattgag tctaaaggga ggagaaagaa gcttcatgag      600

gttggatctg tgactgccat atgtttcacc tgtttcctca ttagatgctt tgtggttgtt      660

ttatccgctt ttgattcaga cgcatctctt gatgtcttgg atcatcccct tctgaatctg      720

atatactacc tgctggtaga aatacttcct tcagcccttg tactgtacat cttacgaaag      780

ttgcctccaa aaagagtatc tgcacaatac cacccaatca gttaa                      825


<210>  95
<211>  871
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TOM3 Petunia hybrida Coding sequence (taken from literature).

<400>  95
atggggcggg cagagatggt ggtaggcccg tcgtccacgg cggtgacgta tcagctgata       60

agttggtggg aagaggtgaa cagatcacgc gcttggcaag accgtatatt ccacgtcctt      120

gctatccttt acggcattgt cgccgccgtt gctcttgttc aattaatacg cattcaaatg      180

agagttcctg aatatggttg gaccactcaa aaagtcttcc acttcctcaa tttcttggtg      240

aatggggttc gctcgctagt gtttgtattt cgtcgggatg ttcagaagtt gcatcctgag      300

attgtccagc atatcttgct tgatatgcca agtcttgcat tcttcacaac ttatgctctg      360

ctagtattat tctgggctga gatatactac caggcacgcg ctgtatccac ggatgggctt      420

agacccagtt tcttcacaat caatggagtg gtttatgcta ttcagattat attatggctg      480

ataatatggt ggaaacctat tcgagtactc gtcattttat ctaagatgtt ctttgcaggt      540

gtatctctat ttgctgcgtt ggggtttctc ctctatggtg gaaggctttt tcttatgtta      600

caacggtttc ctgtggaatc aaaagggaga cgtaagaagc tacaggaggt tggctatgtc      660

acaacaatat gtttttcatg cttcctcatt agatgcgtta tgatgtgctt caatgcattt      720

gataaagctg cagatcttga tgttttggat catccaattt tgaatttgat atattacctg      780

ttagtggaga tactaccttc ttcacttgtc ctttttattt tgaggaagtt acctccaaag      840

cgagggatca cacagtacca ccctattcgc t                                     871


<210>  96
<211>  713
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  96
ctcgaggacg tcagtgtcgt tggtagcatt tgagtttcgg ctgtcagcgc aggggcgccg       60

ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg      120

gcaccgagtc ggtgcttttt ttcctaagga tttaaggacg tgaactctgt tgagatctac      180

atcttgttct ggggtttcac actatcttta gagaaagtgt taagttaatt aagttatctt      240

aattaagagc ataattatac tgatttgtct ctcgttgata gagtctatca ttctgttact      300

aaaaatttga caactcggtt tgctgaccta ctggttactg tatcacttac ccgagttaac      360

gagcctaagg atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg      420

catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc      480

ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc      540

ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc      600

cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat      660

gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc agg             713


<210>  97
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -2bp indel.

<400>  97
tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc ccggttcttt ttgt             54


<210>  98
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -8bp indel.

<400>  98
tgctctgatg ccgccgtgtt ccggctgtca gcgcccggtt ctttttgt                    48


<210>  99
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -36bp indel.

<400>  99
tgctctgatg ttctttttgt                                                   20


<210>  100
<211>  49
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -8bp+1bp indel.

<400>  100
tgctctgatg ccgccgtgtt ccggctgtca gcgcgccggt tctttttgt                   49


<210>  101
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with +1bp indel.

<400>  101
tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc cgcccggttc tttttgt          57


<210>  102
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with +1bp indel.

<400>  102
tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc agcccggttc tttttgt          57


<210>  103
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -3bp indel.

<400>  103
tgctctgatg ccgccgtgtt ccggctgtca gcgcagggcc cggttctttt tgt              53


<210>  104
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -8bp indel.

<400>  104
tgctctgatg ccgccgtgtt ccggctgtca gcgcagggtt ctttttgt                    48


<210>  105
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with +1bp indel.

<400>  105
tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc tgcccggttc tttttgt          57


<210>  106
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -6bp indel.

<400>  106
tgctctgatg ccgccgtgtt ccggctgtca gcgcgcccgg ttctttttgt                  50


<210>  107
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleotide sequence of the target site detected in the N. 
       benthamiana target gene NptII with -8bp indel.


<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  n is a, c, g, or t

<400>  107
tgctctgatg ccgccgtgtt ccggctgtca gcgcagggnt ctttttgt                    48


