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<110>  Wilmar (Shanghai) Biotechnology Research & Development Center Co.,Ltd 
 
<120>  A METHOD FOR RECOMBINANT EXPRESSION OF beta-GLUCOSIDASE GENE BGL1

<130>  143455

<160>  7     

<170>  PatentIn version 3.3

<210>  1
<211>  1483
<212>  DNA
<213>  artificial sequence

<400>  1
gtcggtaatc ccgctgtata gtaatacgag tcgcatctaa atactccgaa gctgctgcga     60
acccggagaa tcgagatgtg ctggaaagct tctagcgagc ggctaaatta gcatgaaagg    120
ctatgagaaa ttctggagac ggcttgttga atcatggcgt tccattcttc gacaagcaaa    180
gcgttccgtc gcagtagcag gcactcattc ccgaaaaaac tcggagattc ctaagtagcg    240
atggaaccgg aataatataa taggcaatac attgagttgc ctcgacggtt gcaatgcagg    300
ggtactgagc ttggacataa ctgttccgta ccccacctct tctcaacctt tggcgtttcc    360
ctgattcagc gtacccgtac aagtcgtaat cactattaac ccagactgac cggacgtgtt    420
ttgcccttca tttggagaaa taatgtcatt gcgatgtgta atttgcctgc ttgaccgact    480
ggggctgttc gaagcccgaa tgtaggattg ttatccgaac tctgctcgta gaggcatgtt    540
gtgaatctgt gtcgggcagg acacgcctcg aaggttcacg gcaagggaaa ccaccgatag    600
cagtgtctag tagcaacctg taaagccgca atgcagcatc actggaaaat acaaaccaat    660
ggctaaaagt acataagtta atgcctaaag gagtcatata ccagcggcta ataattgtac    720
aatcaagtgg ctaaacgtac cgtaatttgc caacggcttg tggggttgca gaagcaacgg    780
caaagcccca cttccccacg tttgtttctt cactcagtcc aatctcagct ggtgatcccc    840
caattgggtc gcttgtttgt tccggtgaag tgaaagaaga cagaggtaag aatgtctgac    900
tcggagcgtt ttgcatacaa ccaagggcag tgatggaaga cagtgaaatg ttgacattca    960
aggagtattt agccagggat gcttgagtgt atcgtgtaag gaggtttgtc tgccgatacg   1020
acgaatactg tatagtcact tctgatgaag tggtccatat tgaaatgtaa gtcggcactg   1080
aacaggcaaa agattgagtt gaaactgcct aagatctcgg gccctcgggc cttcggcctt   1140
tgggtgtaca tgtttgtgct ccgggcaaat gcaaagtgtg gtaggatcga acacactgct   1200
gcctttacca agcagctgag ggtatgtgat aggcaaatgt tcaggggcca ctgcatggtt   1260
tcgaatagaa agagaagctt agccaagaac aatagccgat aaagatagcc tcattaaacg   1320
gaatgagcta gtaggcaaag tcagcgaatg tgtatatata aaggttcgag gtccgtgcct   1380
ccctcatgct ctccccatct actcatcaac tcagatcctc caggagactt gtacaccatc   1440
ttttgaggca cagaaaccca atagtcaacc gcggactggc atc                     1483


<210>  2
<211>  51
<212>  DNA
<213>  artificial sequence

<400>  2
atgtatcgga agttggccgt catctcggcc ttcttggcca cagctcgtgc t              51


<210>  3
<211>  1220
<212>  DNA
<213>  artificial sequence

<400>  3
atgctcttct cttctattgc cattgttgcg gcagcaactg ctgccttggc atcgccggta     60
aagccaagtg ccaagactgc cgcgctatca gtgaagcgtg tctcgaacgt caaatcattg    120
aagaatattg tccaaaaggg ccaagcacgc atcaacaaga tcaatggcgt caaggacatc    180
gaggccagag ctagcggccc agttaccaac gaggatgtta gctatgttgc ctcagtcact    240
atcggtggtc aatcttggga cctcatcgtc gacactggat gtacgtcatc actacataga    300
cactgaacaa cgcatgtgct gacctgatat cctaactagc ttcaaacacg tggtgtggtg    360
cccaacgctc atgcgagcct tcatctactg gcaagtccac gggcggttcc gtccaggtta    420
gctatggttc cggctccttc tccggcaccg agtacaagga cacagttagc ttcggtggtt    480
tgactgtcac atcacagtcg gttggagctg cccgttcatc ctctggcttt tcaggtgtcg    540
atggaattat tggctttggt ccggtggatc tcactgagga caccgtctcc aacgccaaca    600
cggttccaac cttcctggat aatctctaca gccaaggttc catctcgact gaggtgctgg    660
gtgtttcctt caagccagag tctggcagtg acagcgatga caccaacggc gagttgaccc    720
tcggcggtac tgatagctcc aagtacacgg gctctctcac ctacttctca actctcaaga    780
gtggctctgc tgctccctac tggggcatct ctattgctag tttcacctac ggctcgacga    840
ccctcgcatc gtctgcgacc ggcattgtcg acactggtac tacgctcatc tacatcccca    900
ccaaggctta caatgcattc ctgtctgccg ctggtggcaa gactgacagc tcttctggcc    960
tcgccgtctt ctcaaaagcg ccaacatcca actttgctat caagtttggc tcaacgacct   1020
acaccctcac accttctcaa tacttggttc ccaccgctca gtacagcttc tacggactca   1080
gctctggaaa gtactacgct tggattaacg acggtggcag ctcgggtgtg aacaccatta   1140
ttggtcagaa gttcctggaa aactactact ccgtttttga tactaccaac ggccgcatcg   1200
gctttgctac cgcggcttaa                                               1220


<210>  4
<211>  48
<212>  DNA
<213>  artificial sequence

<400>  4
ggatccccag ccactaccac tggaagctct cccggaccta ccacgcgt                  48


<210>  5
<211>  2206
<212>  DNA
<213>  Trichoderma reesei

<400>  5
gttgtacctc ctgcagggac tccatgggga accgcgtacg acaaggcgaa ggccgcattg     60
gcaaagctca atctccaaga taaggtcggc atcgtgagcg gtgtcggctg gaacggcggt    120
ccttgcgttg gaaacacatc tccggcctcc aagatcagct atccatcgct atgccttcaa    180
gacggacccc tcggtgttcg atactcgaca ggcagcacag cctttacgcc gggcgttcaa    240
gcggcctcga cgtgggatgt caatttgatc cgcgaacgtg gacagttcat cggtgaggag    300
gtgaaggcct cggggattca tgtcatactt ggtcctgtgg ctgggccgct gggaaagact    360
ccgcagggcg gtcgcaactg ggagggcttc ggtgtcgatc catatctcac gggcattgcc    420
atgggtcaaa ccatcaacgg catccagtcg gtaggcgtgc aggcgacagc gaagcactat    480
atcctcaacg agcaggagct caatcgagaa accatttcga gcaacccaga tgaccgaact    540
ctccatgagc tgtatacttg gccatttgcc gacgcggttc aggccaatgt cgcttctgtc    600
atgtgctcgt acaacaaggt caataccacc tgggcctgcg aggatcagta cacgctgcag    660
actgtgctga aagaccagct ggggttccca ggctatgtca tgacggactg gaacgcacag    720
cacacgactg tccaaagcgc gaattctggg cttgacatgt caatgcctgg cacagacttc    780
aacggtaaca atcggctctg gggtccagct ctcaccaatg cggtaaatag caatcaggtc    840
cccacgagca gagtcgacga tatggtgact cgtatcctcg ccgcatggta cttgacaggc    900
caggaccagg caggctatcc gtcgttcaac atcagcagaa atgttcaagg aaaccacaag    960
accaatgtca gggcaattgc cagggacggc atcgttctgc tcaagaatga cgccaacatc   1020
ctgccgctca agaagcccgc tagcattgcc gtcgttggat ctgccgcaat cattggtaac   1080
cacgccagaa actcgccctc gtgcaacgac aaaggctgcg acgacggggc cttgggcatg   1140
ggttggggtt ccggcgccgt caactatccg tacttcgtcg cgccctacga tgccatcaat   1200
accagagcgt cttcgcaggg cacccaggtt accttgagca acaccgacaa cacgtcctca   1260
ggcgcatctg cagcaagagg aaaggacgtc gccatcgtct tcatcaccgc cgactcgggt   1320
gaaggctaca tcaccgtgga gggcaacgcg ggcgatcgca acaacctgga tccgtggcac   1380
aacggcaatg ccctggtcca ggcggtggcc ggtgccaaca gcaacgtcat tgttgttgtc   1440
cactccgttg gcgccatcat tctggagcag attcttgctc ttccgcaggt caaggccgtt   1500
gtctgggcgg gtcttccttc tcaggagagc ggcaatgcgc tcgtcgacgt gctgtgggga   1560
gatgtcagcc cttctggcaa gctggtgtac accattgcga agagccccaa tgactataac   1620
actcgcatcg tttccggcgg cagtgacagc ttcagcgagg gactgttcat cgactataag   1680
cacttcgacg acgccaatat cacgccgcgg tacgagttcg gctatggact gtgtaagttt   1740
gctaacctga acaatctatt agacaggttg actgacggat gactgtggaa tgatagctta   1800
caccaagttc aactactcac gcctctccgt cttgtcgacc gccaagtctg gtcctgcgac   1860
tggggccgtt gtgccgggag gcccgagtga tctgttccag aatgtcgcga cagtcaccgt   1920
tgacatcgca aactctggcc aagtgactgg tgccgaggta gcccagctgt acatcaccta   1980
cccatcttca gcacccagga cccctccgaa gcagctgcga ggctttgcca agctgaacct   2040
cacgcctggt cagagcggaa cagcaacgtt caacatccga cgacgagatc tcagctactg   2100
ggacacggct tcgcagaaat gggtggtgcc gtcggggtcg tttggcatca gcgtgggagc   2160
gagcagccgg gatatcaggc tgacgagcac tctgtcggta gcgtaa                  2206


<210>  6
<211>  338
<212>  DNA
<213>  artificial sequence

<400>  6
agctccgtgg cgaaagcctg acgcaccggt agattcttgg tgagcccgta tcatgacggc     60
ggcgggagct acatggcccc gggtgattta ttttttttgt atctacttct gacccttttc    120
aaatatacgg tcaactcatc tttcactgga gatgcggcct gcttggtatt gcgatgttgt    180
cagcttggca aattgtggct ttcgaaaaca caaaacgatt ccttagtagc catgcatttt    240
aagataacgg aatagaagaa agaggaaatt aaaaaaaaaa aaaaaacaaa catcccgttc    300
ataacccgta gaatcgccgc tcttcgtgta tcccagta                            338


<210>  7
<211>  1161
<212>  DNA
<213>  artificial sequence

<400>  7
atgctcttct cctctattgc cattgttgcg gcggcaactg ctgccttggc atcgccggta     60
aagccaagtg ccaagactgc cgcgctatca gtgaagcgtg tctcgaacgt caaatcattg    120
aagaatattg tccaaaaggg ccaggcacgc atcaacaaga tcaacggcgt caaagacatc    180
gaggccagag ctagcggccc agccaccaac gaggatgtta gctatgttgc ctcggtcact    240
attggtggta aatcctggga cctcatcgtc gacactggat cttcaaacac gtggtgtggt    300
gctcaaagct catgcgagcc ttcatctact ggcaagtcca cgggcggttc cgtccaggtc    360
agctatggtt ccggctcctt ctccggcacc gagtacaagg acacagttag cttcggtggt    420
ttgactgtca catcacagtc ggttggagct gcccgttcat cctctggctt ttcaggtgtc    480
gatggaatta ttggctttgg tccggtggat ctcactgagg acaccgtctc caacgccaac    540
acggttccaa ccttcttgga taatctctac agccaaggtt ccatctcgac tgaggtgctg    600
ggcgtttctt tcaagccaga gtctggcagt gacagtgatg acaccaacgg cgagttgacc    660
ctcggcggta ctgatagctc caagtacacg ggctctctca cctacttctc aactctcaag    720
agtggctctg ctgctcccta ctggggcatc tctattgcta gtttcaccta cggctcgacg    780
accctcgcat cgtctgcgac cggcattgtc gacactggta ctacgctcat ctacatcccc    840
accaaggctt acaatgcatt cctgtctgcc gctggtggca agactgacag ctcttctggc    900
ctcgccgtct tctcaaaagc gccaacatcc aactttgcta tcaagtttgg ctcaacgacc    960
tacaccctca caccttctca atacttggtt cccacctctc agtacagctt ctacggactc   1020
agctctggaa agtactacgc ttggattaac gacggtggca gctcgggtgt caacaccatt   1080
attggccaga agttcctgga aaactactac tccgtttttg atactaccaa cggccgcatc   1140
ggctttgcta ccgccgctta a                                             1161
