                         SEQUENCE LISTING

<110>  Sleevi, Mark C
       Lile, Jack
       Grimison, Bryn
 
<120>  PROCESS FOR REFOLDING RECOMBINANT CHYMOTRYPSIN

<130>  23873

<150>  62/081,227
<151>  2014-11-18

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  245
<212>  PRT
<213>  Sus scrufa

<400>  1

Cys Gly Val Pro Ala Ile Pro Pro Val Leu Ser Gly Leu Ser Arg Ile 
1               5                   10                  15      


Val Asn Gly Glu Asn Ala Val Pro Gly Ser Trp Pro Trp Gln Val Ser 
            20                  25                  30          


Leu Gln Asp Gly Thr Gly Phe His Phe Cys Gly Gly Ser Leu Ile Ser 
        35                  40                  45              


Glu Asp Trp Val Val Thr Ala Ala His Cys Gly Val Thr Thr Ser Asp 
    50                  55                  60                  


Val Val Val Ala Gly Glu Tyr Asp Gln Ala Ser Asp Ala Glu Asp Ile 
65                  70                  75                  80  


Gln Val Leu Lys Ile Ala Lys Val Phe Lys Asn Pro Asn Phe Ser Leu 
                85                  90                  95      


Leu Thr Val Arg Asn Asp Ile Thr Leu Leu Lys Leu Ala Thr Pro Ala 
            100                 105                 110         


Arg Phe Ser Arg Thr Val Ser Ala Val Cys Leu Pro Ser Ala Ser Asp 
        115                 120                 125             


Asp Phe Pro Ala Gly Thr Leu Cys Ala Thr Thr Gly Trp Gly Lys Thr 
    130                 135                 140                 


Lys Tyr Thr Ala Leu Lys Thr Pro Asp Lys Leu Gln Gln Ala Ala Leu 
145                 150                 155                 160 


Pro Ile Val Ser Ser Thr Val Cys Lys Ser Tyr Trp Gly Ser Lys Val 
                165                 170                 175     


Thr Asp Val Met Ile Cys Ala Gly Ala Ser Gly Val Ser Ser Cys Met 
            180                 185                 190         


Gly Asp Ser Gly Gly Pro Leu Val Cys Gln Lys Asn Gly Ala Trp Thr 
        195                 200                 205             


Leu Val Gly Ile Val Ser Trp Gly Ser Ser Thr Cys Ser Thr Thr Thr 
    210                 215                 220                 


Pro Ala Val Tyr Ala Arg Val Thr Ala Leu Ile Pro Trp Val Gln Gln 
225                 230                 235                 240 


Ile Leu Ala Asn Asn 
                245 


<210>  2
<211>  230
<212>  PRT
<213>  Sus scrufa

<400>  2

Ile Val Asn Gly Glu Asn Ala Val Pro Gly Ser Trp Pro Trp Gln Val 
1               5                   10                  15      


Ser Leu Gln Asp Gly Thr Gly Phe His Phe Cys Gly Gly Ser Leu Ile 
            20                  25                  30          


Ser Glu Asp Trp Val Val Thr Ala Ala His Cys Gly Val Thr Thr Ser 
        35                  40                  45              


Asp Val Val Val Ala Gly Glu Tyr Asp Gln Ala Ser Asp Ala Glu Asp 
    50                  55                  60                  


Ile Gln Val Leu Lys Ile Ala Lys Val Phe Lys Asn Pro Asn Phe Ser 
65                  70                  75                  80  


Leu Leu Thr Val Arg Asn Asp Ile Thr Leu Leu Lys Leu Ala Thr Pro 
                85                  90                  95      


Ala Arg Phe Ser Arg Thr Val Ser Ala Val Cys Leu Pro Ser Ala Ser 
            100                 105                 110         


Asp Asp Phe Pro Ala Gly Thr Leu Cys Ala Thr Thr Gly Trp Gly Lys 
        115                 120                 125             


Thr Lys Tyr Thr Ala Leu Lys Thr Pro Asp Lys Leu Gln Gln Ala Ala 
    130                 135                 140                 


Leu Pro Ile Val Ser Ser Thr Val Cys Lys Ser Tyr Trp Gly Ser Lys 
145                 150                 155                 160 


Val Thr Asp Val Met Ile Cys Ala Gly Ala Ser Gly Val Ser Ser Cys 
                165                 170                 175     


Met Gly Asp Ser Gly Gly Pro Leu Val Cys Gln Lys Asn Gly Ala Trp 
            180                 185                 190         


Thr Leu Val Gly Ile Val Ser Trp Gly Ser Ser Thr Cys Ser Thr Thr 
        195                 200                 205             


Thr Pro Ala Val Tyr Ala Arg Val Thr Ala Leu Ile Pro Trp Val Gln 
    210                 215                 220                 


Gln Ile Leu Ala Asn Asn 
225                 230 


<210>  3
<211>  254
<212>  PRT
<213>  Artificial sequence

<220>
<223>  modified chymotrypsin B with a leader of 6-8 amino acids


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is any amino acid or absent

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is any amino acid or absent

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  X is F, W, or Y

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Met Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Gly Val Pro Ala Ile Pro 
1               5                   10                  15      


Pro Val Leu Ser Gly Leu Ser Arg Ile Val Asn Gly Glu Asn Ala Val 
            20                  25                  30          


Pro Gly Ser Trp Pro Trp Gln Val Ser Leu Gln Asp Gly Thr Gly Phe 
        35                  40                  45              


His Phe Cys Gly Gly Ser Leu Ile Ser Glu Asp Trp Val Val Thr Ala 
    50                  55                  60                  


Ala His Cys Gly Val Thr Thr Ser Asp Val Val Val Ala Gly Glu Tyr 
65                  70                  75                  80  


Asp Gln Ala Ser Asp Ala Glu Asp Ile Gln Val Leu Lys Ile Ala Lys 
                85                  90                  95      


Val Phe Lys Asn Pro Asn Phe Ser Leu Leu Thr Val Arg Asn Asp Ile 
            100                 105                 110         


Thr Leu Leu Lys Leu Ala Thr Pro Ala Arg Phe Ser Arg Thr Val Ser 
        115                 120                 125             


Ala Val Cys Leu Pro Ser Ala Ser Asp Asp Phe Pro Ala Gly Thr Leu 
    130                 135                 140                 


Cys Ala Thr Thr Gly Trp Gly Lys Thr Lys Tyr Thr Ala Leu Lys Thr 
145                 150                 155                 160 


Pro Asp Lys Leu Gln Gln Ala Ala Leu Pro Ile Val Ser Ser Thr Val 
                165                 170                 175     


Cys Lys Ser Tyr Trp Gly Ser Lys Val Thr Asp Val Met Ile Cys Ala 
            180                 185                 190         


Gly Ala Ser Gly Val Ser Ser Cys Met Gly Asp Ser Gly Gly Pro Leu 
        195                 200                 205             


Val Cys Gln Lys Asn Gly Ala Trp Thr Leu Val Gly Ile Val Ser Trp 
    210                 215                 220                 


Gly Ser Ser Thr Cys Ser Thr Thr Thr Pro Ala Val Tyr Ala Arg Val 
225                 230                 235                 240 


Thr Ala Leu Ile Pro Trp Val Gln Gln Ile Leu Ala Asn Asn 
                245                 250                 


<210>  4
<211>  254
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chymotrypsin B with a 7 amino acid leader


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is any amino acid or absent

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is any amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is any amino acid or absent

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Met Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe Cys Gly Val Pro Ala Ile Pro 
1               5                   10                  15      


Pro Val Leu Ser Gly Leu Ser Arg Ile Val Asn Gly Glu Asn Ala Val 
            20                  25                  30          


Pro Gly Ser Trp Pro Trp Gln Val Ser Leu Gln Asp Gly Thr Gly Phe 
        35                  40                  45              


His Phe Cys Gly Gly Ser Leu Ile Ser Glu Asp Trp Val Val Thr Ala 
    50                  55                  60                  


Ala His Cys Gly Val Thr Thr Ser Asp Val Val Val Ala Gly Glu Tyr 
65                  70                  75                  80  


Asp Gln Ala Ser Asp Ala Glu Asp Ile Gln Val Leu Lys Ile Ala Lys 
                85                  90                  95      


Val Phe Lys Asn Pro Asn Phe Ser Leu Leu Thr Val Arg Asn Asp Ile 
            100                 105                 110         


Thr Leu Leu Lys Leu Ala Thr Pro Ala Arg Phe Ser Arg Thr Val Ser 
        115                 120                 125             


Ala Val Cys Leu Pro Ser Ala Ser Asp Asp Phe Pro Ala Gly Thr Leu 
    130                 135                 140                 


Cys Ala Thr Thr Gly Trp Gly Lys Thr Lys Tyr Thr Ala Leu Lys Thr 
145                 150                 155                 160 


Pro Asp Lys Leu Gln Gln Ala Ala Leu Pro Ile Val Ser Ser Thr Val 
                165                 170                 175     


Cys Lys Ser Tyr Trp Gly Ser Lys Val Thr Asp Val Met Ile Cys Ala 
            180                 185                 190         


Gly Ala Ser Gly Val Ser Ser Cys Met Gly Asp Ser Gly Gly Pro Leu 
        195                 200                 205             


Val Cys Gln Lys Asn Gly Ala Trp Thr Leu Val Gly Ile Val Ser Trp 
    210                 215                 220                 


Gly Ser Ser Thr Cys Ser Thr Thr Thr Pro Ala Val Tyr Ala Arg Val 
225                 230                 235                 240 


Thr Ala Leu Ile Pro Trp Val Gln Gln Ile Leu Ala Asn Asn 
                245                 250                 


