                         SEQUENCE LISTING

<110>  INSTITUT PASTEUR
 
<120>  A live and attenuated Japanese encephalitis virus comprising a 
       mutated M protein

<130>  B10995 - AD/DP

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  225
<212>  DNA
<213>  Japanese encephalitis virus

<400>  1
tccgtgtcgg tccaaacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggatcat aaggaatcct      120

ggctatgctt tcctggcggc ggtacttggc tggatgcttg gcagtaacaa cggtcaacgc      180

gtggtattca ccatcctcct gctgctggtt gctccggctt acagt                      225


<210>  2
<211>  75
<212>  PRT
<213>  Japanese encephalitis virus

<400>  2

Ser Val Ser Val Gln Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Ile Ile Arg Asn Pro Gly Tyr Ala Phe Leu Ala Ala Val 
        35                  40                  45              


Leu Gly Trp Met Leu Gly Ser Asn Asn Gly Gln Arg Val Val Phe Thr 
    50                  55                  60                  


Ile Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  3
<211>  225
<212>  DNA
<213>  Artificial

<220>
<223>  mutated JEV M


<220>
<221>  misc_feature
<222>  (106)..(108)
<223>  n is a, c, g, or t

<400>  3
tccgtgtcgg tccaaacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggnnnat aaggaatcct      120

ggctatgctt tcctggcggc ggtacttggc tggatgcttg gcagtaacaa cggtcaacgc      180

gtggtattca ccatcctcct gctgctggtt gctccggctt acagt                      225


<210>  4
<211>  75
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV M


<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Ser Val Ser Val Gln Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Xaa Ile Arg Asn Pro Gly Tyr Ala Phe Leu Ala Ala Val 
        35                  40                  45              


Leu Gly Trp Met Leu Gly Ser Asn Asn Gly Gln Arg Val Val Phe Thr 
    50                  55                  60                  


Ile Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  5
<211>  120
<212>  DNA
<213>  Artificial

<220>
<223>  mutated JEV ectoM

<400>  5
tccgtgtcgg tccaaacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggatcat aaggaatcct      120


<210>  6
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV ectoM

<400>  6

Ser Val Ser Val Gln Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Ile Ile Arg Asn Pro 
        35                  40  


<210>  7
<211>  120
<212>  DNA
<213>  Artificial

<220>
<223>  mutated JEV ectoM


<220>
<221>  misc_feature
<222>  (106)..(108)
<223>  n is a, c, g, or t

<400>  7
tccgtgtcgg tccaaacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggnnnat aaggaatcct      120


<210>  8
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV ectoM


<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<400>  8

Ser Val Ser Val Gln Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Xaa Ile Arg Asn Pro 
        35                  40  


<210>  9
<211>  225
<212>  DNA
<213>  Artificial

<220>
<223>  mutated JEV M


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (106)..(108)
<223>  n is a, c, g, or t

<400>  9
tccgtgtcgg tcnnnacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggnnnat aaggaatcct      120

ggctatgctt tcctggcggc ggtacttggc tggatgcttg gcagtaacaa cggtcaacgc      180

gtggtattca ccatcctcct gctgctggtt gctccggctt acagt                      225


<210>  10
<211>  75
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV M


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Ser Val Ser Val Xaa Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Xaa Ile Arg Asn Pro Gly Tyr Ala Phe Leu Ala Ala Val 
        35                  40                  45              


Leu Gly Trp Met Leu Gly Ser Asn Asn Gly Gln Arg Val Val Phe Thr 
    50                  55                  60                  


Ile Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  11
<211>  120
<212>  DNA
<213>  Artificial

<220>
<223>  mutated JEV ectoM


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (106)..(108)
<223>  n is a, c, g, or t

<400>  11
tccgtgtcgg tcnnnacaca tggggagagt tcactagtga ataaaaaaga ggcttggctg       60

gattcaacga aagccacacg atatctcatg aaaactgaga actggnnnat aaggaatcct      120


<210>  12
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV ectoM


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Ser Val Ser Val Xaa Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys 
1               5                   10                  15      


Glu Ala Trp Leu Asp Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr 
            20                  25                  30          


Glu Asn Trp Xaa Ile Arg Asn Pro 
        35                  40  


<210>  13
<211>  3432
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV polyprotein

<400>  13

Met Thr Lys Lys Pro Gly Gly Pro Gly Lys Asn Arg Ala Ile Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Leu Pro Arg Val Phe Pro Leu Val Gly Val Lys Arg 
            20                  25                  30          


Val Val Met Ser Leu Leu Asp Gly Arg Gly Pro Val Arg Phe Val Leu 
        35                  40                  45              


Ala Leu Ile Thr Phe Phe Lys Phe Thr Ala Leu Ala Pro Thr Lys Ala 
    50                  55                  60                  


Leu Leu Gly Arg Trp Lys Ala Val Glu Lys Ser Val Ala Met Lys His 
65                  70                  75                  80  


Leu Thr Ser Phe Lys Arg Glu Leu Gly Thr Leu Ile Asp Ala Val Asn 
                85                  90                  95      


Lys Arg Gly Arg Lys Gln Asn Lys Arg Gly Gly Asn Glu Gly Ser Ile 
            100                 105                 110         


Met Trp Leu Ala Ser Leu Ala Val Val Ile Ala Cys Ala Gly Ala Met 
        115                 120                 125             


Lys Leu Ser Asn Phe Gln Gly Lys Leu Leu Met Thr Ile Asn Asn Thr 
    130                 135                 140                 


Asp Ile Ala Asp Val Ile Val Ile Pro Thr Ser Lys Gly Glu Asn Arg 
145                 150                 155                 160 


Cys Trp Val Arg Ala Ile Asp Val Gly Tyr Met Cys Glu Asp Thr Ile 
                165                 170                 175     


Thr Tyr Glu Cys Pro Lys Leu Thr Met Gly Asn Asp Pro Glu Asp Val 
            180                 185                 190         


Asp Cys Trp Cys Asp Asn Gln Glu Val Tyr Val Gln Tyr Gly Arg Cys 
        195                 200                 205             


Thr Arg Thr Arg His Ser Lys Arg Ser Arg Arg Ser Val Ser Val Gln 
    210                 215                 220                 


Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys Glu Ala Trp Leu Asp 
225                 230                 235                 240 


Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr Glu Asn Trp Phe Ile 
                245                 250                 255     


Arg Asn Pro Gly Tyr Ala Phe Leu Ala Ala Val Leu Gly Trp Met Leu 
            260                 265                 270         


Gly Ser Asn Asn Gly Gln Arg Val Val Phe Thr Ile Leu Leu Leu Leu 
        275                 280                 285             


Val Ala Pro Ala Tyr Ser Phe Asn Cys Leu Gly Met Gly Asn Arg Asp 
    290                 295                 300                 


Phe Ile Glu Gly Ala Ser Gly Ala Thr Trp Val Asp Leu Val Leu Glu 
305                 310                 315                 320 


Gly Asp Ser Cys Leu Thr Ile Met Ala Asn Asp Lys Pro Thr Leu Asp 
                325                 330                 335     


Val Arg Met Ile Asn Ile Glu Ala Ser Gln Leu Ala Glu Val Arg Ser 
            340                 345                 350         


Tyr Cys Tyr His Ala Ser Val Thr Asp Ile Ser Thr Val Ala Arg Cys 
        355                 360                 365             


Pro Thr Thr Gly Glu Ala His Asn Glu Lys Arg Ala Asp Ser Ser Tyr 
    370                 375                 380                 


Val Cys Lys Gln Gly Phe Thr Asp Arg Gly Trp Gly Asn Gly Cys Gly 
385                 390                 395                 400 


Leu Phe Gly Lys Gly Ser Ile Asp Thr Cys Ala Lys Phe Ser Cys Thr 
                405                 410                 415     


Ser Lys Ala Ile Gly Arg Thr Ile Gln Pro Glu Asn Ile Lys Tyr Glu 
            420                 425                 430         


Val Gly Ile Phe Val His Gly Thr Thr Thr Ser Glu Asn His Gly Asn 
        435                 440                 445             


Tyr Ser Ala Gln Val Gly Ala Ser Gln Ala Ala Lys Phe Thr Val Thr 
    450                 455                 460                 


Pro Asn Ala Pro Ser Ile Thr Leu Lys Leu Gly Asp Tyr Gly Glu Val 
465                 470                 475                 480 


Thr Leu Asp Cys Glu Pro Arg Ser Gly Leu Asn Thr Glu Ala Phe Tyr 
                485                 490                 495     


Val Met Thr Val Gly Ser Lys Ser Phe Leu Val His Arg Glu Trp Phe 
            500                 505                 510         


His Asp Leu Ala Leu Pro Trp Thr Ser Pro Ser Ser Thr Ala Trp Arg 
        515                 520                 525             


Asn Arg Glu Leu Leu Met Glu Phe Glu Glu Ala His Ala Thr Lys Gln 
    530                 535                 540                 


Ser Val Val Ala Leu Gly Ser Gln Glu Gly Gly Leu His Gln Ala Leu 
545                 550                 555                 560 


Ala Gly Ala Ile Val Val Glu Tyr Ser Ser Ser Val Lys Leu Thr Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Ala Leu Lys Gly 
            580                 585                 590         


Thr Thr Tyr Gly Met Cys Thr Glu Lys Phe Ser Phe Ala Lys Asn Pro 
        595                 600                 605             


Ala Asp Thr Gly His Gly Thr Val Val Ile Glu Leu Ser Tyr Ser Gly 
    610                 615                 620                 


Ser Asp Gly Pro Cys Lys Ile Pro Ile Val Ser Val Ala Ser Leu Asn 
625                 630                 635                 640 


Asp Met Thr Pro Val Gly Arg Leu Val Thr Val Asn Pro Phe Val Ala 
                645                 650                 655     


Thr Ser Ser Ala Asn Ser Lys Val Leu Val Glu Met Glu Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Val Gly Arg Gly Asp Lys Gln Ile Asn His 
        675                 680                 685             


His Trp His Lys Ala Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr 
    690                 695                 700                 


Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile Gly Lys Ala Val His 
                725                 730                 735     


Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn 
        755                 760                 765             


Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val 
    770                 775                 780                 


Leu Val Phe Leu Ala Thr Asn Val His Ala Asp Thr Gly Cys Ala Ile 
785                 790                 795                 800 


Asp Ile Thr Arg Lys Glu Met Arg Cys Gly Ser Gly Ile Phe Val His 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Val Asp Arg Tyr Lys Tyr Leu Pro Glu Thr 
            820                 825                 830         


Pro Arg Ser Leu Ala Lys Ile Val His Lys Ala His Lys Glu Gly Val 
        835                 840                 845             


Cys Gly Val Arg Ser Val Thr Arg Leu Glu His Gln Met Trp Glu Ala 
    850                 855                 860                 


Val Arg Asp Glu Leu Asn Val Leu Leu Lys Glu Asn Ala Val Asp Leu 
865                 870                 875                 880 


Ser Val Val Val Asn Lys Pro Val Gly Arg Tyr Arg Ser Ala Pro Lys 
                885                 890                 895     


Arg Leu Ser Met Thr Gln Glu Lys Phe Glu Met Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Ile Leu Phe Ala Pro Glu Leu Ala Asn Ser Thr Phe Val 
        915                 920                 925             


Val Asp Gly Pro Glu Thr Lys Glu Cys Pro Asp Glu His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Met Gln Ile Glu Asp Phe Gly Phe Gly Ile Thr Ser Thr Arg 
945                 950                 955                 960 


Val Trp Leu Lys Ile Arg Glu Glu Ser Thr Asp Glu Cys Asp Gly Ala 
                965                 970                 975     


Ile Ile Gly Thr Ala Val Lys Gly His Val Ala Val His Ser Asp Leu 
            980                 985                 990         


Ser Tyr Trp Ile Glu Ser Arg Tyr  Asn Asp Thr Trp Lys  Leu Glu Arg 
        995                 1000                 1005             


Ala Val  Phe Gly Glu Val Lys  Ser Cys Thr Trp Pro  Glu Thr His 
    1010                 1015                 1020             


Thr Leu  Trp Gly Asp Gly Val  Glu Glu Ser Glu Leu  Ile Ile Pro 
    1025                 1030                 1035             


His Thr  Val Ala Gly Pro Lys  Ser Lys His Asn Arg  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Lys  Thr Gln Asn Gln Gly  Pro Trp Asp Glu Asn  Gly Ile Val 
    1055                 1060                 1065             


Leu Asp  Phe Asp Tyr Cys Pro  Gly Thr Lys Val Thr  Ile Thr Glu 
    1070                 1075                 1080             


Asp Cys  Gly Lys Arg Gly Pro  Ser Val Arg Thr Thr  Thr Asp Ser 
    1085                 1090                 1095             


Gly Lys  Leu Ile Thr Asp Trp  Cys Cys Arg Ser Cys  Ser Leu Pro 
    1100                 1105                 1110             


Pro Leu  Arg Phe Arg Thr Glu  Asn Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Val Arg His Asp  Glu Thr Thr Leu Val  Arg Ser Gln 
    1130                 1135                 1140             


Val Asp  Ala Phe Asn Gly Glu  Met Val Asp Pro Phe  Gln Leu Gly 
    1145                 1150                 1155             


Leu Leu  Val Met Phe Leu Ala  Thr Gln Glu Val Leu  Arg Lys Arg 
    1160                 1165                 1170             


Trp Thr  Ala Arg Leu Thr Ile  Pro Ala Val Leu Gly  Ala Leu Leu 
    1175                 1180                 1185             


Val Leu  Met Leu Gly Gly Ile  Thr Tyr Thr Asp Leu  Ala Arg Tyr 
    1190                 1195                 1200             


Val Val  Leu Val Ala Ala Ala  Phe Ala Glu Ala Asn  Ser Gly Gly 
    1205                 1210                 1215             


Asp Val  Leu His Leu Ala Leu  Ile Ala Val Phe Lys  Ile Gln Pro 
    1220                 1225                 1230             


Ala Phe  Leu Val Met Asn Met  Leu Ser Thr Arg Trp  Thr Asn Gln 
    1235                 1240                 1245             


Glu Asn  Val Val Leu Val Leu  Gly Ala Ala Phe Phe  Gln Leu Ala 
    1250                 1255                 1260             


Ser Val  Asp Leu Gln Ile Gly  Val His Gly Ile Leu  Asn Ala Ala 
    1265                 1270                 1275             


Ala Ile  Ala Trp Met Ile Val  Arg Ala Ile Thr Phe  Pro Thr Thr 
    1280                 1285                 1290             


Ser Ser  Val Thr Met Pro Val  Leu Ala Leu Leu Thr  Pro Gly Met 
    1295                 1300                 1305             


Arg Ala  Leu Tyr Leu Asp Thr  Tyr Arg Ile Ile Leu  Leu Val Ile 
    1310                 1315                 1320             


Gly Ile  Cys Ser Leu Leu Gln  Glu Arg Lys Lys Thr  Met Ala Lys 
    1325                 1330                 1335             


Lys Lys  Gly Ala Val Leu Leu  Gly Leu Ala Leu Thr  Ser Thr Gly 
    1340                 1345                 1350             


Trp Phe  Ser Pro Thr Thr Ile  Ala Ala Gly Leu Met  Val Cys Asn 
    1355                 1360                 1365             


Pro Asn  Lys Lys Arg Gly Trp  Pro Ala Thr Glu Phe  Leu Ser Ala 
    1370                 1375                 1380             


Val Gly  Leu Met Phe Ala Ile  Val Gly Gly Leu Ala  Glu Leu Asp 
    1385                 1390                 1395             


Ile Glu  Ser Met Ser Ile Pro  Phe Met Leu Ala Gly  Leu Met Ala 
    1400                 1405                 1410             


Val Ser  Tyr Val Val Ser Gly  Lys Ala Thr Asp Met  Trp Leu Glu 
    1415                 1420                 1425             


Arg Ala  Ala Asp Ile Ser Trp  Glu Met Asp Ala Ala  Ile Thr Gly 
    1430                 1435                 1440             


Ser Ser  Arg Arg Leu Asp Val  Lys Leu Asp Glu Asp  Gly Asp Phe 
    1445                 1450                 1455             


His Leu  Ile Asp Asp Pro Gly  Val Pro Trp Lys Val  Trp Val Leu 
    1460                 1465                 1470             


Arg Met  Ser Cys Ile Gly Leu  Ala Ala Leu Thr Pro  Trp Ala Ile 
    1475                 1480                 1485             


Val Pro  Ala Ala Phe Gly Tyr  Trp Leu Thr Leu Lys  Thr Thr Lys 
    1490                 1495                 1500             


Arg Gly  Gly Val Phe Trp Asp  Thr Pro Ser Pro Lys  Pro Cys Ser 
    1505                 1510                 1515             


Lys Gly  Asp Thr Thr Thr Gly  Val Tyr Arg Ile Met  Ala Arg Gly 
    1520                 1525                 1530             


Ile Leu  Gly Thr Tyr Gln Ala  Gly Val Gly Val Met  Tyr Glu Asn 
    1535                 1540                 1545             


Val Phe  His Thr Leu Trp His  Thr Thr Arg Gly Ala  Ala Ile Met 
    1550                 1555                 1560             


Ser Gly  Glu Gly Lys Leu Thr  Pro Tyr Trp Gly Ser  Val Lys Glu 
    1565                 1570                 1575             


Asp Arg  Ile Ala Tyr Gly Gly  Pro Trp Arg Phe Asp  Arg Lys Trp 
    1580                 1585                 1590             


Asn Gly  Thr Asp Asp Val Gln  Val Ile Val Val Glu  Pro Gly Lys 
    1595                 1600                 1605             


Ala Ala  Val Asn Ile Gln Thr  Lys Pro Gly Val Phe  Arg Thr Pro 
    1610                 1615                 1620             


Phe Gly  Glu Val Gly Ala Val  Ser Leu Asp Tyr Pro  Arg Gly Thr 
    1625                 1630                 1635             


Ser Gly  Ser Pro Ile Leu Asp  Ser Asn Gly Asp Ile  Ile Gly Leu 
    1640                 1645                 1650             


Tyr Gly  Asn Gly Val Glu Leu  Gly Asp Gly Ser Tyr  Val Ser Ala 
    1655                 1660                 1665             


Ile Val  Gln Gly Asp Arg Gln  Glu Glu Pro Val Pro  Glu Ala Tyr 
    1670                 1675                 1680             


Thr Pro  Asn Met Leu Arg Lys  Arg Gln Met Thr Val  Leu Asp Leu 
    1685                 1690                 1695             


His Pro  Gly Ser Gly Lys Thr  Arg Lys Ile Leu Pro  Gln Ile Ile 
    1700                 1705                 1710             


Lys Asp  Ala Ile Gln Gln Arg  Leu Arg Thr Ala Val  Leu Ala Pro 
    1715                 1720                 1725             


Thr Arg  Val Val Ala Ala Glu  Met Ala Glu Ala Leu  Arg Gly Leu 
    1730                 1735                 1740             


Pro Val  Arg Tyr Gln Thr Ser  Ala Val Gln Arg Glu  His Gln Gly 
    1745                 1750                 1755             


Asn Glu  Ile Val Asp Val Met  Cys His Ala Thr Leu  Thr His Arg 
    1760                 1765                 1770             


Leu Met  Ser Pro Asn Arg Val  Pro Asn Tyr Asn Leu  Phe Val Met 
    1775                 1780                 1785             


Asp Glu  Ala His Phe Thr Asp  Pro Ala Ser Ile Ala  Ala Arg Gly 
    1790                 1795                 1800             


Tyr Ile  Ala Thr Lys Val Glu  Leu Gly Glu Ala Ala  Ala Ile Phe 
    1805                 1810                 1815             


Met Thr  Ala Thr Pro Pro Gly  Thr Thr Asp Pro Phe  Pro Asp Ser 
    1820                 1825                 1830             


Asn Ala  Pro Ile His Asp Leu  Gln Asp Glu Ile Pro  Asp Arg Ala 
    1835                 1840                 1845             


Trp Ser  Ser Gly Tyr Glu Trp  Ile Thr Glu Tyr Ala  Gly Lys Thr 
    1850                 1855                 1860             


Val Trp  Phe Val Ala Ser Val  Lys Met Gly Asn Glu  Ile Ala Met 
    1865                 1870                 1875             


Cys Leu  Gln Arg Ala Gly Lys  Lys Val Ile Gln Leu  Asn Arg Lys 
    1880                 1885                 1890             


Ser Tyr  Asp Thr Glu Tyr Pro  Lys Cys Lys Asn Gly  Asp Trp Asp 
    1895                 1900                 1905             


Phe Val  Ile Thr Thr Asp Ile  Ser Glu Met Gly Ala  Asn Phe Gly 
    1910                 1915                 1920             


Ala Ser  Arg Val Ile Asp Cys  Arg Lys Ser Val Lys  Pro Thr Ile 
    1925                 1930                 1935             


Leu Glu  Glu Gly Glu Gly Arg  Val Ile Leu Gly Asn  Pro Ser Pro 
    1940                 1945                 1950             


Ile Thr  Ser Ala Ser Ala Ala  Gln Arg Arg Gly Arg  Val Gly Arg 
    1955                 1960                 1965             


Asn Pro  Asn Gln Val Gly Asp  Glu Tyr His Tyr Gly  Gly Ala Thr 
    1970                 1975                 1980             


Ser Glu  Asp Asp Ser Asn Leu  Ala His Trp Thr Glu  Ala Lys Ile 
    1985                 1990                 1995             


Met Leu  Asp Asn Ile His Met  Pro Asn Gly Leu Val  Ala Gln Leu 
    2000                 2005                 2010             


Tyr Gly  Pro Glu Arg Glu Lys  Ala Phe Thr Met Asp  Gly Glu Tyr 
    2015                 2020                 2025             


Arg Leu  Arg Gly Glu Glu Lys  Lys Asn Phe Leu Glu  Leu Leu Arg 
    2030                 2035                 2040             


Thr Ala  Asp Leu Pro Val Trp  Leu Ala Tyr Lys Val  Ala Ser Asn 
    2045                 2050                 2055             


Gly Ile  Gln Tyr Thr Asp Arg  Lys Trp Cys Phe Asp  Gly Pro Arg 
    2060                 2065                 2070             


Thr Asn  Ala Ile Leu Glu Asp  Asn Thr Glu Val Glu  Ile Val Thr 
    2075                 2080                 2085             


Arg Met  Gly Glu Arg Lys Ile  Leu Lys Pro Arg Trp  Leu Asp Ala 
    2090                 2095                 2100             


Arg Val  Tyr Ala Asp His Gln  Ala Leu Lys Trp Phe  Lys Asp Phe 
    2105                 2110                 2115             


Ala Ala  Gly Lys Arg Ser Ala  Val Ser Phe Ile Glu  Val Leu Gly 
    2120                 2125                 2130             


Arg Met  Pro Glu His Phe Met  Gly Lys Thr Arg Glu  Ala Leu Asp 
    2135                 2140                 2145             


Thr Met  Tyr Leu Val Ala Thr  Ala Glu Lys Gly Gly  Lys Ala His 
    2150                 2155                 2160             


Arg Met  Ala Leu Glu Glu Leu  Pro Asp Ala Leu Glu  Thr Ile Thr 
    2165                 2170                 2175             


Leu Ile  Val Ala Ile Thr Val  Met Thr Gly Gly Phe  Phe Leu Leu 
    2180                 2185                 2190             


Met Met  Gln Arg Lys Gly Ile  Gly Lys Met Gly Leu  Gly Ala Leu 
    2195                 2200                 2205             


Val Leu  Thr Leu Ala Thr Phe  Phe Leu Trp Ala Ala  Glu Val Pro 
    2210                 2215                 2220             


Gly Thr  Lys Ile Ala Gly Thr  Leu Leu Ile Ala Leu  Leu Leu Met 
    2225                 2230                 2235             


Val Val  Leu Ile Pro Glu Pro  Glu Lys Gln Arg Ser  Gln Thr Asp 
    2240                 2245                 2250             


Asn Gln  Leu Ala Val Phe Leu  Ile Cys Val Leu Thr  Val Val Gly 
    2255                 2260                 2265             


Val Val  Ala Ala Asn Glu Tyr  Gly Met Leu Glu Lys  Thr Lys Ala 
    2270                 2275                 2280             


Asp Leu  Lys Ser Met Phe Gly  Gly Lys Thr Gln Ala  Ser Gly Leu 
    2285                 2290                 2295             


Thr Gly  Leu Pro Ser Met Ala  Leu Asp Leu Arg Pro  Ala Thr Ala 
    2300                 2305                 2310             


Trp Ala  Leu Tyr Gly Gly Ser  Thr Val Val Leu Thr  Pro Leu Leu 
    2315                 2320                 2325             


Lys His  Leu Ile Thr Ser Glu  Tyr Val Thr Thr Ser  Leu Ala Ser 
    2330                 2335                 2340             


Ile Asn  Ser Gln Ala Gly Ser  Leu Phe Val Leu Pro  Arg Gly Val 
    2345                 2350                 2355             


Pro Phe  Thr Asp Leu Asp Leu  Thr Val Gly Leu Val  Phe Leu Gly 
    2360                 2365                 2370             


Cys Trp  Gly Gln Ile Thr Leu  Thr Thr Phe Leu Thr  Ala Met Val 
    2375                 2380                 2385             


Leu Ala  Thr Leu His Tyr Gly  Tyr Met Leu Pro Gly  Trp Gln Ala 
    2390                 2395                 2400             


Glu Ala  Leu Arg Ala Ala Gln  Arg Arg Thr Ala Ala  Gly Ile Met 
    2405                 2410                 2415             


Lys Asn  Ala Val Val Asp Gly  Met Val Ala Thr Asp  Val Pro Glu 
    2420                 2425                 2430             


Leu Glu  Arg Thr Thr Pro Leu  Met Gln Lys Lys Val  Gly Gln Val 
    2435                 2440                 2445             


Leu Leu  Ile Gly Val Ser Val  Ala Ala Phe Leu Val  Asn Pro Asn 
    2450                 2455                 2460             


Val Thr  Thr Val Arg Glu Ala  Gly Val Leu Val Thr  Ala Ala Thr 
    2465                 2470                 2475             


Leu Thr  Leu Trp Asp Asn Gly  Ala Ser Ala Val Trp  Asn Ser Thr 
    2480                 2485                 2490             


Thr Ala  Thr Gly Leu Cys His  Val Met Arg Gly Ser  Tyr Leu Ala 
    2495                 2500                 2505             


Gly Gly  Ser Ile Ala Trp Thr  Leu Ile Lys Asn Ala  Asp Lys Pro 
    2510                 2515                 2520             


Ser Leu  Lys Arg Gly Arg Pro  Gly Gly Arg Thr Leu  Gly Glu Gln 
    2525                 2530                 2535             


Trp Lys  Glu Lys Leu Asn Ala  Met Ser Arg Glu Glu  Phe Phe Lys 
    2540                 2545                 2550             


Tyr Arg  Arg Glu Ala Ile Ile  Glu Val Asp Arg Thr  Glu Ala Arg 
    2555                 2560                 2565             


Arg Ala  Arg Arg Glu Asn Asn  Ile Val Gly Gly His  Pro Val Ser 
    2570                 2575                 2580             


Arg Gly  Ser Ala Lys Leu Arg  Trp Leu Val Glu Lys  Gly Phe Val 
    2585                 2590                 2595             


Ser Pro  Ile Gly Lys Val Ile  Asp Leu Gly Cys Gly  Arg Gly Gly 
    2600                 2605                 2610             


Trp Ser  Tyr Tyr Ala Ala Thr  Leu Lys Lys Val Gln  Glu Val Arg 
    2615                 2620                 2625             


Gly Tyr  Thr Lys Gly Gly Ala  Gly His Glu Glu Pro  Met Leu Met 
    2630                 2635                 2640             


Gln Ser  Tyr Gly Trp Asn Leu  Val Ser Leu Lys Ser  Gly Val Asp 
    2645                 2650                 2655             


Val Phe  Tyr Lys Pro Ser Glu  Pro Ser Asp Thr Leu  Phe Cys Asp 
    2660                 2665                 2670             


Ile Gly  Glu Ser Ser Pro Ser  Pro Glu Val Glu Glu  Gln Arg Thr 
    2675                 2680                 2685             


Leu Arg  Val Leu Glu Met Thr  Ser Asp Trp Leu His  Arg Gly Pro 
    2690                 2695                 2700             


Arg Glu  Phe Cys Ile Lys Val  Leu Cys Pro Tyr Met  Pro Lys Val 
    2705                 2710                 2715             


Ile Glu  Lys Met Glu Val Leu  Gln Arg Arg Phe Gly  Gly Gly Leu 
    2720                 2725                 2730             


Val Arg  Leu Pro Leu Ser Arg  Asn Ser Asn His Glu  Met Tyr Trp 
    2735                 2740                 2745             


Val Ser  Gly Ala Ala Gly Asn  Val Val His Ala Val  Asn Met Thr 
    2750                 2755                 2760             


Ser Gln  Val Leu Leu Gly Arg  Met Asp Arg Thr Val  Trp Arg Gly 
    2765                 2770                 2775             


Pro Lys  Tyr Glu Glu Asp Val  Asn Leu Gly Ser Gly  Thr Arg Ala 
    2780                 2785                 2790             


Val Gly  Lys Gly Glu Val His  Ser Asn Gln Glu Lys  Ile Lys Lys 
    2795                 2800                 2805             


Arg Ile  Gln Lys Leu Lys Glu  Glu Phe Ala Thr Thr  Trp His Lys 
    2810                 2815                 2820             


Asp Pro  Glu His Pro Tyr Arg  Thr Trp Thr Tyr His  Gly Ser Tyr 
    2825                 2830                 2835             


Glu Val  Lys Ala Thr Gly Ser  Ala Ser Ser Leu Val  Asn Gly Val 
    2840                 2845                 2850             


Val Lys  Leu Met Ser Lys Pro  Trp Asp Ala Ile Ala  Asn Val Thr 
    2855                 2860                 2865             


Thr Met  Ala Met Thr Asp Thr  Thr Pro Phe Gly Gln  Gln Arg Val 
    2870                 2875                 2880             


Phe Lys  Glu Lys Val Asp Thr  Lys Ala Pro Glu Pro  Pro Ala Gly 
    2885                 2890                 2895             


Ala Lys  Glu Val Leu Asn Glu  Thr Thr Asn Trp Leu  Trp Ala Tyr 
    2900                 2905                 2910             


Leu Ser  Arg Glu Lys Arg Pro  Arg Leu Cys Thr Lys  Glu Glu Phe 
    2915                 2920                 2925             


Ile Lys  Lys Val Asn Ser Asn  Ala Ala Leu Gly Ala  Val Phe Ala 
    2930                 2935                 2940             


Glu Gln  Asn Gln Trp Ser Thr  Ala Arg Glu Ala Val  Asp Asp Pro 
    2945                 2950                 2955             


Arg Phe  Trp Glu Met Val Asp  Glu Glu Arg Glu Asn  His Leu Arg 
    2960                 2965                 2970             


Gly Glu  Cys His Thr Cys Ile  Tyr Asn Met Met Gly  Lys Arg Glu 
    2975                 2980                 2985             


Lys Lys  Pro Gly Glu Phe Gly  Lys Ala Lys Gly Ser  Arg Ala Ile 
    2990                 2995                 3000             


Trp Phe  Met Trp Leu Gly Ala  Arg Tyr Leu Glu Phe  Glu Ala Leu 
    3005                 3010                 3015             


Gly Phe  Leu Asn Glu Asp His  Trp Leu Ser Arg Glu  Asn Ser Gly 
    3020                 3025                 3030             


Gly Gly  Val Glu Gly Ser Gly  Val Gln Lys Leu Gly  Tyr Ile Leu 
    3035                 3040                 3045             


Arg Asp  Ile Ala Gly Lys Gln  Gly Gly Lys Met Tyr  Ala Asp Asp 
    3050                 3055                 3060             


Thr Ala  Gly Trp Asp Thr Arg  Ile Thr Arg Thr Asp  Leu Glu Asn 
    3065                 3070                 3075             


Glu Ala  Lys Val Leu Glu Leu  Leu Asp Gly Glu His  Arg Met Leu 
    3080                 3085                 3090             


Ala Arg  Ala Ile Ile Glu Leu  Thr Tyr Arg His Lys  Val Val Lys 
    3095                 3100                 3105             


Val Met  Arg Pro Ala Ala Glu  Gly Lys Thr Val Met  Asp Val Ile 
    3110                 3115                 3120             


Ser Arg  Glu Asp Gln Arg Gly  Ser Gly Gln Val Val  Thr Tyr Ala 
    3125                 3130                 3135             


Leu Asn  Thr Phe Thr Asn Ile  Ala Val Gln Leu Val  Arg Leu Met 
    3140                 3145                 3150             


Glu Ala  Glu Gly Val Ile Gly  Pro Gln His Leu Glu  Gln Leu Pro 
    3155                 3160                 3165             


Arg Lys  Asn Lys Ile Ala Val  Arg Thr Trp Leu Phe  Glu Asn Gly 
    3170                 3175                 3180             


Glu Glu  Arg Val Thr Arg Met  Ala Ile Ser Gly Asp  Asp Cys Val 
    3185                 3190                 3195             


Val Lys  Pro Leu Asp Asp Arg  Phe Ala Thr Ala Leu  His Phe Leu 
    3200                 3205                 3210             


Asn Ala  Met Ser Lys Val Arg  Lys Asp Ile Gln Glu  Trp Lys Pro 
    3215                 3220                 3225             


Ser His  Gly Trp His Asp Trp  Gln Gln Val Pro Phe  Cys Ser Asn 
    3230                 3235                 3240             


His Phe  Gln Glu Ile Val Met  Lys Asp Gly Arg Ser  Ile Val Val 
    3245                 3250                 3255             


Pro Cys  Arg Gly Gln Asp Glu  Leu Ile Gly Arg Ala  Arg Ile Ser 
    3260                 3265                 3270             


Pro Gly  Ala Gly Trp Asn Val  Lys Asp Thr Ala Cys  Leu Ala Lys 
    3275                 3280                 3285             


Ala Tyr  Ala Gln Met Trp Leu  Leu Leu Tyr Phe His  Arg Arg Asp 
    3290                 3295                 3300             


Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser Ala Val  Pro Val Asp 
    3305                 3310                 3315             


Trp Val  Pro Thr Gly Arg Thr  Ser Trp Ser Ile His  Ser Lys Gly 
    3320                 3325                 3330             


Glu Trp  Met Thr Thr Glu Asp  Met Leu Gln Val Trp  Asn Arg Val 
    3335                 3340                 3345             


Trp Ile  Glu Glu Asn Glu Trp  Met Met Asp Lys Thr  Pro Ile Thr 
    3350                 3355                 3360             


Ser Trp  Thr Asp Val Pro Tyr  Val Gly Lys Arg Glu  Asp Ile Trp 
    3365                 3370                 3375             


Cys Gly  Ser Leu Ile Gly Thr  Arg Ser Arg Ala Thr  Trp Ala Glu 
    3380                 3385                 3390             


Asn Ile  Tyr Ala Ala Ile Asn  Gln Val Arg Ala Val  Ile Gly Lys 
    3395                 3400                 3405             


Glu Asn  Tyr Val Asp Tyr Met  Thr Ser Leu Arg Arg  Tyr Glu Asp 
    3410                 3415                 3420             


Val Leu  Ile Gln Glu Asp Arg  Val Ile 
    3425                 3430         


<210>  14
<211>  3432
<212>  PRT
<213>  Artificial

<220>
<223>  mutated JEV polyprotein

<400>  14

Met Thr Lys Lys Pro Gly Gly Pro Gly Lys Asn Arg Ala Ile Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Leu Pro Arg Val Phe Pro Leu Val Gly Val Lys Arg 
            20                  25                  30          


Val Val Met Ser Leu Leu Asp Gly Arg Gly Pro Val Arg Phe Val Leu 
        35                  40                  45              


Ala Leu Ile Thr Phe Phe Lys Phe Thr Ala Leu Ala Pro Thr Lys Ala 
    50                  55                  60                  


Leu Leu Gly Arg Trp Lys Ala Val Glu Lys Ser Val Ala Met Lys His 
65                  70                  75                  80  


Leu Thr Ser Phe Lys Arg Glu Leu Gly Thr Leu Ile Asp Ala Val Asn 
                85                  90                  95      


Lys Arg Gly Arg Lys Gln Asn Lys Arg Gly Gly Asn Glu Gly Ser Ile 
            100                 105                 110         


Met Trp Leu Ala Ser Leu Ala Val Val Ile Ala Cys Ala Gly Ala Met 
        115                 120                 125             


Lys Leu Ser Asn Phe Gln Gly Lys Leu Leu Met Thr Ile Asn Asn Thr 
    130                 135                 140                 


Asp Ile Ala Asp Val Ile Val Ile Pro Thr Ser Lys Gly Glu Asn Arg 
145                 150                 155                 160 


Cys Trp Val Arg Ala Ile Asp Val Gly Tyr Met Cys Glu Asp Thr Ile 
                165                 170                 175     


Thr Tyr Glu Cys Pro Lys Leu Thr Met Gly Asn Asp Pro Glu Asp Val 
            180                 185                 190         


Asp Cys Trp Cys Asp Asn Gln Glu Val Tyr Val Gln Tyr Gly Arg Cys 
        195                 200                 205             


Thr Arg Thr Arg His Ser Lys Arg Ser Arg Arg Ser Val Ser Val Pro 
    210                 215                 220                 


Thr His Gly Glu Ser Ser Leu Val Asn Lys Lys Glu Ala Trp Leu Asp 
225                 230                 235                 240 


Ser Thr Lys Ala Thr Arg Tyr Leu Met Lys Thr Glu Asn Trp Phe Ile 
                245                 250                 255     


Arg Asn Pro Gly Tyr Ala Phe Leu Ala Ala Val Leu Gly Trp Met Leu 
            260                 265                 270         


Gly Ser Asn Asn Gly Gln Arg Val Val Phe Thr Ile Leu Leu Leu Leu 
        275                 280                 285             


Val Ala Pro Ala Tyr Ser Phe Asn Cys Leu Gly Met Gly Asn Arg Asp 
    290                 295                 300                 


Phe Ile Glu Gly Ala Ser Gly Ala Thr Trp Val Asp Leu Val Leu Glu 
305                 310                 315                 320 


Gly Asp Ser Cys Leu Thr Ile Met Ala Asn Asp Lys Pro Thr Leu Asp 
                325                 330                 335     


Val Arg Met Ile Asn Ile Glu Ala Ser Gln Leu Ala Glu Val Arg Ser 
            340                 345                 350         


Tyr Cys Tyr His Ala Ser Val Thr Asp Ile Ser Thr Val Ala Arg Cys 
        355                 360                 365             


Pro Thr Thr Gly Glu Ala His Asn Glu Lys Arg Ala Asp Ser Ser Tyr 
    370                 375                 380                 


Val Cys Lys Gln Gly Phe Thr Asp Arg Gly Trp Gly Asn Gly Cys Gly 
385                 390                 395                 400 


Leu Phe Gly Lys Gly Ser Ile Asp Thr Cys Ala Lys Phe Ser Cys Thr 
                405                 410                 415     


Ser Lys Ala Ile Gly Arg Thr Ile Gln Pro Glu Asn Ile Lys Tyr Glu 
            420                 425                 430         


Val Gly Ile Phe Val His Gly Thr Thr Thr Ser Glu Asn His Gly Asn 
        435                 440                 445             


Tyr Ser Ala Gln Val Gly Ala Ser Gln Ala Ala Lys Phe Thr Val Thr 
    450                 455                 460                 


Pro Asn Ala Pro Ser Ile Thr Leu Lys Leu Gly Asp Tyr Gly Glu Val 
465                 470                 475                 480 


Thr Leu Asp Cys Glu Pro Arg Ser Gly Leu Asn Thr Glu Ala Phe Tyr 
                485                 490                 495     


Val Met Thr Val Gly Ser Lys Ser Phe Leu Val His Arg Glu Trp Phe 
            500                 505                 510         


His Asp Leu Ala Leu Pro Trp Thr Ser Pro Ser Ser Thr Ala Trp Arg 
        515                 520                 525             


Asn Arg Glu Leu Leu Met Glu Phe Glu Glu Ala His Ala Thr Lys Gln 
    530                 535                 540                 


Ser Val Val Ala Leu Gly Ser Gln Glu Gly Gly Leu His Gln Ala Leu 
545                 550                 555                 560 


Ala Gly Ala Ile Val Val Glu Tyr Ser Ser Ser Val Lys Leu Thr Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Ala Leu Lys Gly 
            580                 585                 590         


Thr Thr Tyr Gly Met Cys Thr Glu Lys Phe Ser Phe Ala Lys Asn Pro 
        595                 600                 605             


Ala Asp Thr Gly His Gly Thr Val Val Ile Glu Leu Ser Tyr Ser Gly 
    610                 615                 620                 


Ser Asp Gly Pro Cys Lys Ile Pro Ile Val Ser Val Ala Ser Leu Asn 
625                 630                 635                 640 


Asp Met Thr Pro Val Gly Arg Leu Val Thr Val Asn Pro Phe Val Ala 
                645                 650                 655     


Thr Ser Ser Ala Asn Ser Lys Val Leu Val Glu Met Glu Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Val Gly Arg Gly Asp Lys Gln Ile Asn His 
        675                 680                 685             


His Trp His Lys Ala Gly Ser Thr Leu Gly Lys Ala Phe Ser Thr Thr 
    690                 695                 700                 


Leu Lys Gly Ala Gln Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Ile Gly Gly Val Phe Asn Ser Ile Gly Lys Ala Val His 
                725                 730                 735     


Gln Val Phe Gly Gly Ala Phe Arg Thr Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Ile Thr Gln Gly Leu Met Gly Ala Leu Leu Leu Trp Met Gly Val Asn 
        755                 760                 765             


Ala Arg Asp Arg Ser Ile Ala Leu Ala Phe Leu Ala Thr Gly Gly Val 
    770                 775                 780                 


Leu Val Phe Leu Ala Thr Asn Val His Ala Asp Thr Gly Cys Ala Ile 
785                 790                 795                 800 


Asp Ile Thr Arg Lys Glu Met Arg Cys Gly Ser Gly Ile Phe Val His 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Val Asp Arg Tyr Lys Tyr Leu Pro Glu Thr 
            820                 825                 830         


Pro Arg Ser Leu Ala Lys Ile Val His Lys Ala His Lys Glu Gly Val 
        835                 840                 845             


Cys Gly Val Arg Ser Val Thr Arg Leu Glu His Gln Met Trp Glu Ala 
    850                 855                 860                 


Val Arg Asp Glu Leu Asn Val Leu Leu Lys Glu Asn Ala Val Asp Leu 
865                 870                 875                 880 


Ser Val Val Val Asn Lys Pro Val Gly Arg Tyr Arg Ser Ala Pro Lys 
                885                 890                 895     


Arg Leu Ser Met Thr Gln Glu Lys Phe Glu Met Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Ile Leu Phe Ala Pro Glu Leu Ala Asn Ser Thr Phe Val 
        915                 920                 925             


Val Asp Gly Pro Glu Thr Lys Glu Cys Pro Asp Glu His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Met Gln Ile Glu Asp Phe Gly Phe Gly Ile Thr Ser Thr Arg 
945                 950                 955                 960 


Val Trp Leu Lys Ile Arg Glu Glu Ser Thr Asp Glu Cys Asp Gly Ala 
                965                 970                 975     


Ile Ile Gly Thr Ala Val Lys Gly His Val Ala Val His Ser Asp Leu 
            980                 985                 990         


Ser Tyr Trp Ile Glu Ser Arg Tyr  Asn Asp Thr Trp Lys  Leu Glu Arg 
        995                 1000                 1005             


Ala Val  Phe Gly Glu Val Lys  Ser Cys Thr Trp Pro  Glu Thr His 
    1010                 1015                 1020             


Thr Leu  Trp Gly Asp Gly Val  Glu Glu Ser Glu Leu  Ile Ile Pro 
    1025                 1030                 1035             


His Thr  Val Ala Gly Pro Lys  Ser Lys His Asn Arg  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Lys  Thr Gln Asn Gln Gly  Pro Trp Asp Glu Asn  Gly Ile Val 
    1055                 1060                 1065             


Leu Asp  Phe Asp Tyr Cys Pro  Gly Thr Lys Val Thr  Ile Thr Glu 
    1070                 1075                 1080             


Asp Cys  Gly Lys Arg Gly Pro  Ser Val Arg Thr Thr  Thr Asp Ser 
    1085                 1090                 1095             


Gly Lys  Leu Ile Thr Asp Trp  Cys Cys Arg Ser Cys  Ser Leu Pro 
    1100                 1105                 1110             


Pro Leu  Arg Phe Arg Thr Glu  Asn Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Val Arg His Asp  Glu Thr Thr Leu Val  Arg Ser Gln 
    1130                 1135                 1140             


Val Asp  Ala Phe Asn Gly Glu  Met Val Asp Pro Phe  Gln Leu Gly 
    1145                 1150                 1155             


Leu Leu  Val Met Phe Leu Ala  Thr Gln Glu Val Leu  Arg Lys Arg 
    1160                 1165                 1170             


Trp Thr  Ala Arg Leu Thr Ile  Pro Ala Val Leu Gly  Ala Leu Leu 
    1175                 1180                 1185             


Val Leu  Met Leu Gly Gly Ile  Thr Tyr Thr Asp Leu  Ala Arg Tyr 
    1190                 1195                 1200             


Val Val  Leu Val Ala Ala Ala  Phe Ala Glu Ala Asn  Ser Gly Gly 
    1205                 1210                 1215             


Asp Val  Leu His Leu Ala Leu  Ile Ala Val Phe Lys  Ile Gln Pro 
    1220                 1225                 1230             


Ala Phe  Leu Val Met Asn Met  Leu Ser Thr Arg Trp  Thr Asn Gln 
    1235                 1240                 1245             


Glu Asn  Val Val Leu Val Leu  Gly Ala Ala Phe Phe  Gln Leu Ala 
    1250                 1255                 1260             


Ser Val  Asp Leu Gln Ile Gly  Val His Gly Ile Leu  Asn Ala Ala 
    1265                 1270                 1275             


Ala Ile  Ala Trp Met Ile Val  Arg Ala Ile Thr Phe  Pro Thr Thr 
    1280                 1285                 1290             


Ser Ser  Val Thr Met Pro Val  Leu Ala Leu Leu Thr  Pro Gly Met 
    1295                 1300                 1305             


Arg Ala  Leu Tyr Leu Asp Thr  Tyr Arg Ile Ile Leu  Leu Val Ile 
    1310                 1315                 1320             


Gly Ile  Cys Ser Leu Leu Gln  Glu Arg Lys Lys Thr  Met Ala Lys 
    1325                 1330                 1335             


Lys Lys  Gly Ala Val Leu Leu  Gly Leu Ala Leu Thr  Ser Thr Gly 
    1340                 1345                 1350             


Trp Phe  Ser Pro Thr Thr Ile  Ala Ala Gly Leu Met  Val Cys Asn 
    1355                 1360                 1365             


Pro Asn  Lys Lys Arg Gly Trp  Pro Ala Thr Glu Phe  Leu Ser Ala 
    1370                 1375                 1380             


Val Gly  Leu Met Phe Ala Ile  Val Gly Gly Leu Ala  Glu Leu Asp 
    1385                 1390                 1395             


Ile Glu  Ser Met Ser Ile Pro  Phe Met Leu Ala Gly  Leu Met Ala 
    1400                 1405                 1410             


Val Ser  Tyr Val Val Ser Gly  Lys Ala Thr Asp Met  Trp Leu Glu 
    1415                 1420                 1425             


Arg Ala  Ala Asp Ile Ser Trp  Glu Met Asp Ala Ala  Ile Thr Gly 
    1430                 1435                 1440             


Ser Ser  Arg Arg Leu Asp Val  Lys Leu Asp Glu Asp  Gly Asp Phe 
    1445                 1450                 1455             


His Leu  Ile Asp Asp Pro Gly  Val Pro Trp Lys Val  Trp Val Leu 
    1460                 1465                 1470             


Arg Met  Ser Cys Ile Gly Leu  Ala Ala Leu Thr Pro  Trp Ala Ile 
    1475                 1480                 1485             


Val Pro  Ala Ala Phe Gly Tyr  Trp Leu Thr Leu Lys  Thr Thr Lys 
    1490                 1495                 1500             


Arg Gly  Gly Val Phe Trp Asp  Thr Pro Ser Pro Lys  Pro Cys Ser 
    1505                 1510                 1515             


Lys Gly  Asp Thr Thr Thr Gly  Val Tyr Arg Ile Met  Ala Arg Gly 
    1520                 1525                 1530             


Ile Leu  Gly Thr Tyr Gln Ala  Gly Val Gly Val Met  Tyr Glu Asn 
    1535                 1540                 1545             


Val Phe  His Thr Leu Trp His  Thr Thr Arg Gly Ala  Ala Ile Met 
    1550                 1555                 1560             


Ser Gly  Glu Gly Lys Leu Thr  Pro Tyr Trp Gly Ser  Val Lys Glu 
    1565                 1570                 1575             


Asp Arg  Ile Ala Tyr Gly Gly  Pro Trp Arg Phe Asp  Arg Lys Trp 
    1580                 1585                 1590             


Asn Gly  Thr Asp Asp Val Gln  Val Ile Val Val Glu  Pro Gly Lys 
    1595                 1600                 1605             


Ala Ala  Val Asn Ile Gln Thr  Lys Pro Gly Val Phe  Arg Thr Pro 
    1610                 1615                 1620             


Phe Gly  Glu Val Gly Ala Val  Ser Leu Asp Tyr Pro  Arg Gly Thr 
    1625                 1630                 1635             


Ser Gly  Ser Pro Ile Leu Asp  Ser Asn Gly Asp Ile  Ile Gly Leu 
    1640                 1645                 1650             


Tyr Gly  Asn Gly Val Glu Leu  Gly Asp Gly Ser Tyr  Val Ser Ala 
    1655                 1660                 1665             


Ile Val  Gln Gly Asp Arg Gln  Glu Glu Pro Val Pro  Glu Ala Tyr 
    1670                 1675                 1680             


Thr Pro  Asn Met Leu Arg Lys  Arg Gln Met Thr Val  Leu Asp Leu 
    1685                 1690                 1695             


His Pro  Gly Ser Gly Lys Thr  Arg Lys Ile Leu Pro  Gln Ile Ile 
    1700                 1705                 1710             


Lys Asp  Ala Ile Gln Gln Arg  Leu Arg Thr Ala Val  Leu Ala Pro 
    1715                 1720                 1725             


Thr Arg  Val Val Ala Ala Glu  Met Ala Glu Ala Leu  Arg Gly Leu 
    1730                 1735                 1740             


Pro Val  Arg Tyr Gln Thr Ser  Ala Val Gln Arg Glu  His Gln Gly 
    1745                 1750                 1755             


Asn Glu  Ile Val Asp Val Met  Cys His Ala Thr Leu  Thr His Arg 
    1760                 1765                 1770             


Leu Met  Ser Pro Asn Arg Val  Pro Asn Tyr Asn Leu  Phe Val Met 
    1775                 1780                 1785             


Asp Glu  Ala His Phe Thr Asp  Pro Ala Ser Ile Ala  Ala Arg Gly 
    1790                 1795                 1800             


Tyr Ile  Ala Thr Lys Val Glu  Leu Gly Glu Ala Ala  Ala Ile Phe 
    1805                 1810                 1815             


Met Thr  Ala Thr Pro Pro Gly  Thr Thr Asp Pro Phe  Pro Asp Ser 
    1820                 1825                 1830             


Asn Ala  Pro Ile His Asp Leu  Gln Asp Glu Ile Pro  Asp Arg Ala 
    1835                 1840                 1845             


Trp Ser  Ser Gly Tyr Glu Trp  Ile Thr Glu Tyr Ala  Gly Lys Thr 
    1850                 1855                 1860             


Val Trp  Phe Val Ala Ser Val  Lys Met Gly Asn Glu  Ile Ala Met 
    1865                 1870                 1875             


Cys Leu  Gln Arg Ala Gly Lys  Lys Val Ile Gln Leu  Asn Arg Lys 
    1880                 1885                 1890             


Ser Tyr  Asp Thr Glu Tyr Pro  Lys Cys Lys Asn Gly  Asp Trp Asp 
    1895                 1900                 1905             


Phe Val  Ile Thr Thr Asp Ile  Ser Glu Met Gly Ala  Asn Phe Gly 
    1910                 1915                 1920             


Ala Ser  Arg Val Ile Asp Cys  Arg Lys Ser Val Lys  Pro Thr Ile 
    1925                 1930                 1935             


Leu Glu  Glu Gly Glu Gly Arg  Val Ile Leu Gly Asn  Pro Ser Pro 
    1940                 1945                 1950             


Ile Thr  Ser Ala Ser Ala Ala  Gln Arg Arg Gly Arg  Val Gly Arg 
    1955                 1960                 1965             


Asn Pro  Asn Gln Val Gly Asp  Glu Tyr His Tyr Gly  Gly Ala Thr 
    1970                 1975                 1980             


Ser Glu  Asp Asp Ser Asn Leu  Ala His Trp Thr Glu  Ala Lys Ile 
    1985                 1990                 1995             


Met Leu  Asp Asn Ile His Met  Pro Asn Gly Leu Val  Ala Gln Leu 
    2000                 2005                 2010             


Tyr Gly  Pro Glu Arg Glu Lys  Ala Phe Thr Met Asp  Gly Glu Tyr 
    2015                 2020                 2025             


Arg Leu  Arg Gly Glu Glu Lys  Lys Asn Phe Leu Glu  Leu Leu Arg 
    2030                 2035                 2040             


Thr Ala  Asp Leu Pro Val Trp  Leu Ala Tyr Lys Val  Ala Ser Asn 
    2045                 2050                 2055             


Gly Ile  Gln Tyr Thr Asp Arg  Lys Trp Cys Phe Asp  Gly Pro Arg 
    2060                 2065                 2070             


Thr Asn  Ala Ile Leu Glu Asp  Asn Thr Glu Val Glu  Ile Val Thr 
    2075                 2080                 2085             


Arg Met  Gly Glu Arg Lys Ile  Leu Lys Pro Arg Trp  Leu Asp Ala 
    2090                 2095                 2100             


Arg Val  Tyr Ala Asp His Gln  Ala Leu Lys Trp Phe  Lys Asp Phe 
    2105                 2110                 2115             


Ala Ala  Gly Lys Arg Ser Ala  Val Ser Phe Ile Glu  Val Leu Gly 
    2120                 2125                 2130             


Arg Met  Pro Glu His Phe Met  Gly Lys Thr Arg Glu  Ala Leu Asp 
    2135                 2140                 2145             


Thr Met  Tyr Leu Val Ala Thr  Ala Glu Lys Gly Gly  Lys Ala His 
    2150                 2155                 2160             


Arg Met  Ala Leu Glu Glu Leu  Pro Asp Ala Leu Glu  Thr Ile Thr 
    2165                 2170                 2175             


Leu Ile  Val Ala Ile Thr Val  Met Thr Gly Gly Phe  Phe Leu Leu 
    2180                 2185                 2190             


Met Met  Gln Arg Lys Gly Ile  Gly Lys Met Gly Leu  Gly Ala Leu 
    2195                 2200                 2205             


Val Leu  Thr Leu Ala Thr Phe  Phe Leu Trp Ala Ala  Glu Val Pro 
    2210                 2215                 2220             


Gly Thr  Lys Ile Ala Gly Thr  Leu Leu Ile Ala Leu  Leu Leu Met 
    2225                 2230                 2235             


Val Val  Leu Ile Pro Glu Pro  Glu Lys Gln Arg Ser  Gln Thr Asp 
    2240                 2245                 2250             


Asn Gln  Leu Ala Val Phe Leu  Ile Cys Val Leu Thr  Val Val Gly 
    2255                 2260                 2265             


Val Val  Ala Ala Asn Glu Tyr  Gly Met Leu Glu Lys  Thr Lys Ala 
    2270                 2275                 2280             


Asp Leu  Lys Ser Met Phe Gly  Gly Lys Thr Gln Ala  Ser Gly Leu 
    2285                 2290                 2295             


Thr Gly  Leu Pro Ser Met Ala  Leu Asp Leu Arg Pro  Ala Thr Ala 
    2300                 2305                 2310             


Trp Ala  Leu Tyr Gly Gly Ser  Thr Val Val Leu Thr  Pro Leu Leu 
    2315                 2320                 2325             


Lys His  Leu Ile Thr Ser Glu  Tyr Val Thr Thr Ser  Leu Ala Ser 
    2330                 2335                 2340             


Ile Asn  Ser Gln Ala Gly Ser  Leu Phe Val Leu Pro  Arg Gly Val 
    2345                 2350                 2355             


Pro Phe  Thr Asp Leu Asp Leu  Thr Val Gly Leu Val  Phe Leu Gly 
    2360                 2365                 2370             


Cys Trp  Gly Gln Ile Thr Leu  Thr Thr Phe Leu Thr  Ala Met Val 
    2375                 2380                 2385             


Leu Ala  Thr Leu His Tyr Gly  Tyr Met Leu Pro Gly  Trp Gln Ala 
    2390                 2395                 2400             


Glu Ala  Leu Arg Ala Ala Gln  Arg Arg Thr Ala Ala  Gly Ile Met 
    2405                 2410                 2415             


Lys Asn  Ala Val Val Asp Gly  Met Val Ala Thr Asp  Val Pro Glu 
    2420                 2425                 2430             


Leu Glu  Arg Thr Thr Pro Leu  Met Gln Lys Lys Val  Gly Gln Val 
    2435                 2440                 2445             


Leu Leu  Ile Gly Val Ser Val  Ala Ala Phe Leu Val  Asn Pro Asn 
    2450                 2455                 2460             


Val Thr  Thr Val Arg Glu Ala  Gly Val Leu Val Thr  Ala Ala Thr 
    2465                 2470                 2475             


Leu Thr  Leu Trp Asp Asn Gly  Ala Ser Ala Val Trp  Asn Ser Thr 
    2480                 2485                 2490             


Thr Ala  Thr Gly Leu Cys His  Val Met Arg Gly Ser  Tyr Leu Ala 
    2495                 2500                 2505             


Gly Gly  Ser Ile Ala Trp Thr  Leu Ile Lys Asn Ala  Asp Lys Pro 
    2510                 2515                 2520             


Ser Leu  Lys Arg Gly Arg Pro  Gly Gly Arg Thr Leu  Gly Glu Gln 
    2525                 2530                 2535             


Trp Lys  Glu Lys Leu Asn Ala  Met Ser Arg Glu Glu  Phe Phe Lys 
    2540                 2545                 2550             


Tyr Arg  Arg Glu Ala Ile Ile  Glu Val Asp Arg Thr  Glu Ala Arg 
    2555                 2560                 2565             


Arg Ala  Arg Arg Glu Asn Asn  Ile Val Gly Gly His  Pro Val Ser 
    2570                 2575                 2580             


Arg Gly  Ser Ala Lys Leu Arg  Trp Leu Val Glu Lys  Gly Phe Val 
    2585                 2590                 2595             


Ser Pro  Ile Gly Lys Val Ile  Asp Leu Gly Cys Gly  Arg Gly Gly 
    2600                 2605                 2610             


Trp Ser  Tyr Tyr Ala Ala Thr  Leu Lys Lys Val Gln  Glu Val Arg 
    2615                 2620                 2625             


Gly Tyr  Thr Lys Gly Gly Ala  Gly His Glu Glu Pro  Met Leu Met 
    2630                 2635                 2640             


Gln Ser  Tyr Gly Trp Asn Leu  Val Ser Leu Lys Ser  Gly Val Asp 
    2645                 2650                 2655             


Val Phe  Tyr Lys Pro Ser Glu  Pro Ser Asp Thr Leu  Phe Cys Asp 
    2660                 2665                 2670             


Ile Gly  Glu Ser Ser Pro Ser  Pro Glu Val Glu Glu  Gln Arg Thr 
    2675                 2680                 2685             


Leu Arg  Val Leu Glu Met Thr  Ser Asp Trp Leu His  Arg Gly Pro 
    2690                 2695                 2700             


Arg Glu  Phe Cys Ile Lys Val  Leu Cys Pro Tyr Met  Pro Lys Val 
    2705                 2710                 2715             


Ile Glu  Lys Met Glu Val Leu  Gln Arg Arg Phe Gly  Gly Gly Leu 
    2720                 2725                 2730             


Val Arg  Leu Pro Leu Ser Arg  Asn Ser Asn His Glu  Met Tyr Trp 
    2735                 2740                 2745             


Val Ser  Gly Ala Ala Gly Asn  Val Val His Ala Val  Asn Met Thr 
    2750                 2755                 2760             


Ser Gln  Val Leu Leu Gly Arg  Met Asp Arg Thr Val  Trp Arg Gly 
    2765                 2770                 2775             


Pro Lys  Tyr Glu Glu Asp Val  Asn Leu Gly Ser Gly  Thr Arg Ala 
    2780                 2785                 2790             


Val Gly  Lys Gly Glu Val His  Ser Asn Gln Glu Lys  Ile Lys Lys 
    2795                 2800                 2805             


Arg Ile  Gln Lys Leu Lys Glu  Glu Phe Ala Thr Thr  Trp His Lys 
    2810                 2815                 2820             


Asp Pro  Glu His Pro Tyr Arg  Thr Trp Thr Tyr His  Gly Ser Tyr 
    2825                 2830                 2835             


Glu Val  Lys Ala Thr Gly Ser  Ala Ser Ser Leu Val  Asn Gly Val 
    2840                 2845                 2850             


Val Lys  Leu Met Ser Lys Pro  Trp Asp Ala Ile Ala  Asn Val Thr 
    2855                 2860                 2865             


Thr Met  Ala Met Thr Asp Thr  Thr Pro Phe Gly Gln  Gln Arg Val 
    2870                 2875                 2880             


Phe Lys  Glu Lys Val Asp Thr  Lys Ala Pro Glu Pro  Pro Ala Gly 
    2885                 2890                 2895             


Ala Lys  Glu Val Leu Asn Glu  Thr Thr Asn Trp Leu  Trp Ala Tyr 
    2900                 2905                 2910             


Leu Ser  Arg Glu Lys Arg Pro  Arg Leu Cys Thr Lys  Glu Glu Phe 
    2915                 2920                 2925             


Ile Lys  Lys Val Asn Ser Asn  Ala Ala Leu Gly Ala  Val Phe Ala 
    2930                 2935                 2940             


Glu Gln  Asn Gln Trp Ser Thr  Ala Arg Glu Ala Val  Asp Asp Pro 
    2945                 2950                 2955             


Arg Phe  Trp Glu Met Val Asp  Glu Glu Arg Glu Asn  His Leu Arg 
    2960                 2965                 2970             


Gly Glu  Cys His Thr Cys Ile  Tyr Asn Met Met Gly  Lys Arg Glu 
    2975                 2980                 2985             


Lys Lys  Pro Gly Glu Phe Gly  Lys Ala Lys Gly Ser  Arg Ala Ile 
    2990                 2995                 3000             


Trp Phe  Met Trp Leu Gly Ala  Arg Tyr Leu Glu Phe  Glu Ala Leu 
    3005                 3010                 3015             


Gly Phe  Leu Asn Glu Asp His  Trp Leu Ser Arg Glu  Asn Ser Gly 
    3020                 3025                 3030             


Gly Gly  Val Glu Gly Ser Gly  Val Gln Lys Leu Gly  Tyr Ile Leu 
    3035                 3040                 3045             


Arg Asp  Ile Ala Gly Lys Gln  Gly Gly Lys Met Tyr  Ala Asp Asp 
    3050                 3055                 3060             


Thr Ala  Gly Trp Asp Thr Arg  Ile Thr Arg Thr Asp  Leu Glu Asn 
    3065                 3070                 3075             


Glu Ala  Lys Val Leu Glu Leu  Leu Asp Gly Glu His  Arg Met Leu 
    3080                 3085                 3090             


Ala Arg  Ala Ile Ile Glu Leu  Thr Tyr Arg His Lys  Val Val Lys 
    3095                 3100                 3105             


Val Met  Arg Pro Ala Ala Glu  Gly Lys Thr Val Met  Asp Val Ile 
    3110                 3115                 3120             


Ser Arg  Glu Asp Gln Arg Gly  Ser Gly Gln Val Val  Thr Tyr Ala 
    3125                 3130                 3135             


Leu Asn  Thr Phe Thr Asn Ile  Ala Val Gln Leu Val  Arg Leu Met 
    3140                 3145                 3150             


Glu Ala  Glu Gly Val Ile Gly  Pro Gln His Leu Glu  Gln Leu Pro 
    3155                 3160                 3165             


Arg Lys  Asn Lys Ile Ala Val  Arg Thr Trp Leu Phe  Glu Asn Gly 
    3170                 3175                 3180             


Glu Glu  Arg Val Thr Arg Met  Ala Ile Ser Gly Asp  Asp Cys Val 
    3185                 3190                 3195             


Val Lys  Pro Leu Asp Asp Arg  Phe Ala Thr Ala Leu  His Phe Leu 
    3200                 3205                 3210             


Asn Ala  Met Ser Lys Val Arg  Lys Asp Ile Gln Glu  Trp Lys Pro 
    3215                 3220                 3225             


Ser His  Gly Trp His Asp Trp  Gln Gln Val Pro Phe  Cys Ser Asn 
    3230                 3235                 3240             


His Phe  Gln Glu Ile Val Met  Lys Asp Gly Arg Ser  Ile Val Val 
    3245                 3250                 3255             


Pro Cys  Arg Gly Gln Asp Glu  Leu Ile Gly Arg Ala  Arg Ile Ser 
    3260                 3265                 3270             


Pro Gly  Ala Gly Trp Asn Val  Lys Asp Thr Ala Cys  Leu Ala Lys 
    3275                 3280                 3285             


Ala Tyr  Ala Gln Met Trp Leu  Leu Leu Tyr Phe His  Arg Arg Asp 
    3290                 3295                 3300             


Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser Ala Val  Pro Val Asp 
    3305                 3310                 3315             


Trp Val  Pro Thr Gly Arg Thr  Ser Trp Ser Ile His  Ser Lys Gly 
    3320                 3325                 3330             


Glu Trp  Met Thr Thr Glu Asp  Met Leu Gln Val Trp  Asn Arg Val 
    3335                 3340                 3345             


Trp Ile  Glu Glu Asn Glu Trp  Met Met Asp Lys Thr  Pro Ile Thr 
    3350                 3355                 3360             


Ser Trp  Thr Asp Val Pro Tyr  Val Gly Lys Arg Glu  Asp Ile Trp 
    3365                 3370                 3375             


Cys Gly  Ser Leu Ile Gly Thr  Arg Ser Arg Ala Thr  Trp Ala Glu 
    3380                 3385                 3390             


Asn Ile  Tyr Ala Ala Ile Asn  Gln Val Arg Ala Val  Ile Gly Lys 
    3395                 3400                 3405             


Glu Asn  Tyr Val Asp Tyr Met  Thr Ser Leu Arg Arg  Tyr Glu Asp 
    3410                 3415                 3420             


Val Leu  Ile Gln Glu Asp Arg  Val Ile 
    3425                 3430         


<210>  15
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  15
catgaaaact gagaactggt tcataaggaa tcctggcta                              39


<210>  16
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  16
tagccaggat tccttatgaa ccagttctca gttttcatg                              39


