<110> Universidad de Santiago de Chile 
<120> Plasmido y metodo para obtener particulas virales
<130> CL3146-2014 
<140> 
<141> 
<150> CL 3146-2014 
<151> 2014-11-20 
<160> 1
<210> 1 
<211> 576 
<212> DNA 
<213> Salmo salar 
<220> 
<221> 
<222> 
<400> 1 
Gctagcacgg gtttgccacc cgccggcatg gggctgcgct ccacaaacca aactctgctg 60 tgggtcgggt agggtagggg gctcacgcct cccgcctctc ccttccctcg gcgcgggtga 120 actggtccta gcccggttcc ccgcagttcc tttttgcctg ggatgcgccc aactggctcc 180 atcccctttc cccgttaggc acggctagat gacgcaccga tgggtgggtg tgtaggccgc 240 taccgagggg actgggggtg tccggtgaac cgggacttcc cgaaatggtc tcacattttt 300 aagcggcttg agtatcaccc agtatcttcg cgcggcactg ggaacccagt caaccgctct 360 gcgccccggc gcaggcgggg gtttaatgtc tccccggccc caccggcgct tcggcgacgg 420 cggcagagga gcacccggag gcccccataa agttaaacct acctgtcttt gaactatgac 480 ctctcgctct ggcgaagggc gggcagagga acggagggca acctcccatc tctgccttag 540 cctatagcct ctgcgtaaaa ctggacaaaa aagagta

<210> 2 
<211> 44 
<212> DNA
<213> Escherichia coli
<400> 2 
ttttactctg atgaggcctt cgggccgaaa cggtgaaagc cgta
<210> 3 
<211> 24 
<212> DNA
<213> desconocido
<213> unsure
<213> Sitio de multiple clonamiento
<400> 3 
agaagagccc ggactcgctc ttct
<210> 4 
<211> 84 
<212> DNA
<213> Hepatitis delta virus
<400> 4 
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacattccg aggggaccgt 60 cccctcggta atggcgaatg ggac
<210> 5 
<211> 163
<212> DNA
<213> Oryctolagus sp.
<400> 5 
gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcggaa ggacatatgg gagggcaaat catttaagtc gac 

<210> 6 
<211> 893
<212> DNA
<213> Infectious salmon anemia virus
<400> 6 
gctagcacgg gtttgccacc cgccggcatg gggctgcgct ccacaaacca aactctgctg 60 tgggtcgggt agggtagggg gctcacgcct cccgcctctc ccttccctcg gcgcgggtga 120 actggtccta gcccggttcc ccgcagttcc tttttgcctg ggatgcgccc aactggctcc 180 atcccctttc cccgttaggc acggctagat gacgcaccga tgggtgggtg tgtaggccgc 240 taccgagggg actgggggtg tccggtgaac cgggacttcc cgaaatggtc tcacattttt 300 aagcggcttg agtatcaccc agtatcttcg cgcggcactg ggaacccagt caaccgctct 360 gcgccccggc gcaggcgggg gtttaatgtc tccccggccc caccggcgct tcggcgacgg 420 cggcagagga gcacccggag gcccccataa agttaaacct acctgtcttt gaactatgac 480 ctctcgctct ggcgaagggc gggcagagga acggagggca acctcccatc tctgccttag 540 cctatagcct ctgcgtaaaa ctggacaaaa aagagtattt ttactctgat gaggccttcg 600 ggccgaaacg gtgaaagccg taagaagagc ccggactcgc tcttctggcc ggcatggtcc 660 cagcctcctc gctggcgccg gctgggcaac attccgaggg gaccgtcccc tcggtaatgg 720 cgaatgggac gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 780 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 840 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaagtc gac
<210> 7 
<211> 2169 
<212> DNA
<213> Infectious salmon anemia virus
<400> 7 
atggacttta tatcagaaaa cacgatcagc gacaaaacca cgctagaaga actgaaaaat 60 gctactttat tccaagttac taaagtagac gacagggact gtctaaaagc aagaagaata 120 tgtaatgcac ctaaagggca ctgggctggt ctaatggaaa aagccaaggc aatgggagac 180 ccaactgaag aggaaaaaga tgaattaaag aaaatagtcg agtcatacaa cacagtatca 240 gtgctgggtg tgaccaagtc agaaggagca acaggaccaa ggctggtctc tagtttgaaa 300 ggactaaaaa accttctacc tggagtaaac cccaagacac tacaggaaac acttctggta 360 ggggcaccat gcccctcaac ggaaccaact accgaggagt actggaatgt gtgtagagca 420 gcagttggag catcaatggg atcagcaaag atcaacatgt ctcagaaggt ggtgatggga 480 gctagcgtca tcggatgggg ccaactgaac caaagcggtc caggagtgta tttcttaaac 540 actaaggaaa tagttacagc agaagggaag gtagacgaaa caagaggacc ccttgaaaga 600 acatcagctc ctctaatgag agacatatcc aggctcattc aagaaacaat agaagaagtt 660 gaaacaggag gagatccctc cttttcagta agaagtgaag gaggatcgaa gattgaagga 720 agaattgcat tctctctcca ttcagaagtg tcaacattga aaatgagaat agcactggag 780 caaaaactgg ctaaatacga gtacatggga gagaacttgt tgacacttgt caagaacacg 840 tcaatagaca ggatgcagcc ggactctgct atgatgggga agatggtgct ggaaagcctg 900  agaacgcaca ctgtttcatc tgagcagctg aatgggagga tgatcacagt acagtctcaa 960 ggattggaaa ccatagcaat atcaagtccg ttcgatgtgg aatacgatga tggatacgtt 1020 ttcacgagga tgaaagggaa ctttgttgca atcggacgag attacaaagg ggccatattg 1080 tgtttcagag aaggtcaagg aacattcttc agcggaagag gaaattggtc aggactgatg 1140 gaaaaatgtc tggttgagat gagactgtgt ccgtgcttct acagctgcac atggcaagac 1200 tacccagata agaagagtct gtacgaaaag gcaaccttcg aagccaaaca gatagtgttt 1260 gcaatgggag aaaacacagg ggtggacata agagtgaaca cagacggtga gatcggagat 1320 aaaggaatat cgctactcac aagagagaga gaagataagt acatgtcaaa ggtgtcatac 1380 gaatgcagag ttgtgagcgg aaaactggtt atgggtctgg acaagatgag cagagtagcg 1440 aaggggaact tagaagtagt tagggagaaa ggagatgata ctagtcagtc agactctttt 1500 tacgagggag ttttacaagt gggtagcatg ataggcacaa cgatggaaag cctcaaacaa 1560 caacttcaag gacctgtcgg aatctggaga gcttcagggg tgtctgctat ggagaggtgt 1620 atgaagagag gtcaaagtaa gacagtggtg gcaagtgcaa gatacacttt ccagaaaatg 1680 atggagaaga tggcaacagg tagagaagtg tcgaagtaca gcttgattat tgtcatgaga 1740 tgctgcattg gtttcacttc agaagctaac aaaagagcac tcaccaacat ctcaggaaca 1800 ggttactaca ttagtgttgc acagccaaca gtggtgaaac tagccggaga atggttgatc 1860 accccagtgg gtagatccaa aactggtgag gtgcaatatg tttcggccaa actgaagaag 1920 ggaatgacca caggaaaact agaactgatt aaaaaagcag acagatcaga tctggacaat 1980 tttcctgaac catcagcaga tgaactgctg agagagggaa cgatcgtgct aatgcaaata 2040 gggaaagaca agtggctttg cagagtaaga acaggggaca gaagagttag gacagacaca 2100 gacatacaaa gagccgaagc caagtcacaa gtcgaaaaag aagacctgat ggatgaatat 2160 ggtgtttaa
<210> 8 
<211> 2127 
<212> DNA
<213> Infectious salmon anemia virus
<400> 8 
atggaaactc tagtaggtgg gctgctgact ggggaagatt ctctggtcag tatgtcgaat 60 gatgtgtctt gtctttacgt ttatgatgga ccaatgaggg ttttttcaca aaacgcacta 120 atgcctactt tgcaaagtgt gaagagaagt gaccagttct caaaagggaa aacaaagaga 180 ttcattatag atttgtttgg aatgaaaagg atgtgggaca tagggaacaa acatctagaa 240 gatgaaaatt tggacgaaac tgttggtgtt gcagacttag gtcttgtgaa atacctgata 300 aacaacaagt acgatgaggc agagaaaact agcttaagga agtcaatgga agaagctttc 360 gagaagtcta tgaacgaaga atttgtcgta ctgaacaaag ggaaatcagc taatgacatt 420 atctccgaca caaacgccat gtgtaaattc tgcgttaaaa actggatagt agcaacaggt 480 ttcagaggaa gaaccatgtc agacctgatt gaacaccatt tcaggtgtat gcaggggaaa 540 caagaagtga aaggatacat atggaaacat aaatacaacg agagacttaa gagaaaacaa 600 ctaagcaaag aagaagttaa gttcgacaga gaagaataca cctcaagaag cttcagacta 660 ctttcattcc tgaaaaacag cgaaagaact aagctggagc cgagagcagt gttcacagct 720 ggagtacctt ggagggcctt catcttcgtt ctagaacaga caatgctcgt cgtgaacaag 780 ttggacccaa actcagtcat ctggatggga agcgatgcca aaatcaacac aacaaactcc 840 aggataaagg agatcggaat gaagaaccaa gggcaaacac tggtaacgct tactggagac 900 aactctaagt acaatgagag catgtgccca gaagtgatga tgattttcct aagagaactt 960 gggataaagg gaccaatgct ggaagtactc gactatgctt tgtggcagtt ttctcaaaaa 1020 agtgtcaaac cagtagcacc tataaagaaa agaacaagca ggtctactgt tgtagtgaag 1080 gcggacgctg ttaacgaatg cagggacgct ttcaatgaga aggagctgga actcatacaa 1140 ggagtagagt ggatggaaga tggattcatc agagtgagaa gaggaatgtt gatgggaatg 1200 gcaaacaatg ctttcacaac ggcatctacg attgcttctt ctttcagttt cacacctgag 1260 gcagtgtaca cactacaaag ttcggatgac ttcgtgactg ggagctgtgg gagagacgta 1320 caacatgcaa gacaaagact cgaaatggct cttaaagtga gcaaagctgc aggtttgaac 1380 gtttctcaga agaagtcttt ctatgtggag gggacgactt tcgagttcaa ctctatgttt 1440 gtaagagacg ggaaagtcat ggcaaatgga ggcaactttg aaaacataac agttccaggt 1500 ggacttggac cctcaacaga tctatttgtt gttgggaaac aggcgagaaa ctccatgttg 1560 agaggaaacc tctctttcag ccaagccatg gaaatgtgca aaattggaat cacaaatgtt 1620 gaaaaggtgt actatgggaa cagaaaatac caagaactga agaacgaaat cagagaaaaa 1680 tgtggagaag agacaatgtc tatacctgag agcatgggag gtgataggaa acccaaacct 1740 tgggaacttc ctcagagttt cgatggaatt gcactcaaag aagcagtaaa caggggacac 1800 tggaaggccg ccaagtacat aaaatcttgt tgcagcattg agttcgacga ggaaggagac 1860 cagtcctggg acacgtccaa aacggcacta gtggtaatca gaaaaaacga aactgacatg 1920 agaagaagaa cagttaaaac aaggaaccct aaggacaaga tcttcaatga tgcaatgaac 1980 aaggccaaga ggatgtacga aacagtggtg gacagaaatc ctttgcttgg acttaagggg 2040 aaaggtggga gactgactgt aaaggacctg aaagcaagaa agcttatcga cgaggtggag 2100 gtgattaaga agaaaaagcg tgtttga
<210> 9
<211> 1737 
<212> DNA
<213> Infectious salmon anemia virus
<400> 9 
atggataacc tccgtgaatg cataaaccgc aaaagaagac tacttgcact accagatgta 60 cctgaaactt cagacgcttt cctaagtgat ttgaggcatc tatacatgtg cgttgctttt 120 tgtgaccaac acagaaccac tggagacgag gcaaggttca caaacctgga actgctcgat 180 caagacgaag cgctgggtgc acagagagct ttcgaggcca aacatggaat caaaggaggg 240 tcactaggag acgtgttaga ccatgagctc aaaaaggtga ttgaattcac ttttacttca 300 ggaagtcttt acattgcaga gcaaagaaaa aggaaaactc aagcagactc catcattgta 360  tgtgtgtcag aaggactaaa tgacttcagc gtgtcacacg gtgtactaga catgggtcta 420 gtcgaaacag gagttaatgc agtaagagac ttttgcactc aaaatggaat accaatgaag 480 atcaatcagg taggatccac aagaacaccc acaccgatca gcacatgcag catttccgaa 540 cagataacaa gacagataaa cagcactatt actgacagaa aaatggagaa tgtcctggca 600 gcaattgcaa ccaaaccgga actcaaatta acacagaaag ggtgcagacc ctgcaaagaa 660 ttagaagacg aaaacgtgtt atggatggac ccgcagttct gtgaaattga tgaaagtttt 720 ccttacagag gagggccata cgggaacttc ctgcaagaat tgctgctcac aaccaacgac 780 gtagagacca acgggaaaga cagagaagag gtagtcaaga agatactgga taataaggcc 840 tttactgttg aaagcggcga atgtatcatc acactacctg acaaaatgac ttgttttggc 900 gaacaggaga agaaaaaacc agcgacatta gatgagataa gagtggcagg agagaggttc 960 gaacccagtg tgaaacctaa agcacaaaga tacggaaagc tatcagagaa gtggctagaa 1020 cttgaaaagt tcatttttac tgctagcaag acagaagtcg acacattcct ttcggtggga 1080 actgagaggc ttgagtcaat tggcgtgtgc gtaggtgctt tacacagggc gacaacaacg 1140 cggataatca gaccaatgat acagggagga aaatgctggg ggatgatgtt caagaccaag 1200 tcaaagatgg gagacacaag aaaggaagga tactgccatg caataatctt tgggaaagga 1260 gaggataaat caggccaaaa caggatgact atgatgggga agacagtata ctggcatttg 1320 agagttgtaa agtccaaagg ggactggatg gcacaacaac tgtgtgcaaa caaaagcagg 1380 atctggcaac atgacccgga gctcgtcaca gagggagtta cagtgctgat gacacctttc 1440 tcacagaaga tagctacaat tagcagatgg agagcaatga ggcttgatag catgtttcac 1500 gtctcaagtg catggcacca ttcaccagca tgtgaagctg cttcagcaat gctcaggaag 1560 tttgttgaga tagtacacgc aataaaccag aggagagact ggggtgttgt gggaagcatg 1620 gaggacatgg tcaaggaagt tgaagaaata ggcgaacatc ttcagacggc ttgtgatttt 1680 agggtacata acatgtgtaa agccttgatt cagaaaattg cagtcagtac ccaatga

<210> 10
<211> 1851 
<212> DNA
<213> Infectious salmon anemia virus
<400> 10 
atggccgata aaggtatgac ttattctttt gatgtcagag acaacacctt ggttgtaaga 60 agatctaccg ctactaaaag tggtataaag atctcctaca gagaagatag aggtacttca 120 cttctccaaa aggcctttgc ggggacagat gatgaattct ggctggagct ggatcaagat 180 gtgtacgtag acaagaggac tagaaagttc cttgaggaag agaagatgaa ggacatgagt 240 cccagagtgt ctggttctgt cgctgcagca atcgaaaggt cagtggagtt cgacaacttt 300 tcaaaggaag catctgcgaa catcgagatg tcaggagagg acgaggaaga agcaggtggg 360 agtggcatgg ttgacaacaa gaggaggaac aaaggggtat ccaacatggc gtacaacctt 420 tcactgttca ttggaatggt cttccctgca atcaccacgt tcttcagcgc aatcctgtca 480 gaaggtgaaa tgagcatctg gcaaaacggg caggcaatca tgaggattct cgctttggct 540 gatgaagacg gaaagaggca aacgagaaca ggcggacaga gagtggacat ggcagatgtt 600 accaagctaa acgtggtgac agcaaacggg aaagtcaagc aggttgaagt caatttgaat 660 gacctgaagg cagctttcag acagagcaga cccaaaaggt cagactacag gaaaggacaa 720 ggatcaaagg caactgaatc aagtatttcc aaccagtgca tggctctgat tatgaagtca 780 gtgttgtcag cagaccagct gtttgcacca ggtgtgaaga tgatgaggac caatggtttc 840 aacgcatcat acactacact agcagaagga gccaacattc caagcaagta cctaaggcac 900 atgaggaact gcggaggagt tgctctagat cttatgggga tgaagaggat caagaattca 960 ccggaaggag ctaagtctaa gatcttttct atcattcaga agaaagtcag gggaaggtgt 1020 cgcactgagg agcaacggct gctgactagt gcattgaaga tcagtgatgg cgagaacaag 1080 ttccaaagga tcatggacac tctgtgcaca agctttctga ttgacccacc tagaacaacc 1140 aaatgcttca ttccacctat ctctagtctc ttgatgtaca ttcaggacgg aaattcagtg 1200 ttggcaatgg acttcatgaa gaacggagaa gatgcttgca ggatctgcag agaggcaaag 1260 ctgaaggttg gagtgaacgg cacattcaca atgtctgtgg ctagaacatg tgtagctgtg 1320 tcaatggttg caacagcatt ctgttcagca gatatcatcg agaatgcagt tcccggctca 1380 gaaaggtaca ggtcaaacat caaggcaaac acaaccaaac ccaagaagga ctcaacatac 1440 acaatccaag ggctgaggct gtccaatgtg aagtacgagg caagacctga aacatctcaa 1500 agcaacacag atcgaagctg gcaagtgaat gtcacagaca gtttcggagg gctagcggtt 1560 ttcaaccaag gcgctatcag ggagatgctt ggagatggaa catctgagac aacaagtgtg 1620 aatgtcaggg ctctggtaaa gaggatcttg aagtctgctt cagaaagaag ctcaagagct 1680 gtaaagacat tcatggttgg agaacaaggg aagtctgcaa ttgttatctc aggagtgggg 1740 cttttctcta ttgactttga aggggttgag gaggcagaga ggatcactga catgacacct 1800 gacatcgagt tcgatgagga cgatgaggag gaggaagaca ctgacatttg a
