
                                SEQUENCE LISTING

<110> INVISTA North America S..r.l.

<120> Biosynthesis of 1,3-butanediol
  

<130> 35643-0079WO1

<150> US 62/079,954       
<151> 2014-11-14  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 394
<212> PRT
<213> Cupriavidus necator

<400> 1
Met Thr Arg Glu Val Val Val Val Ser Gly Val Arg Thr Ala Ile Gly
 1               5                  10                  15      
Thr Phe Gly Gly Ser Leu Lys Asp Val Ala Pro Ala Glu Leu Gly Ala
            20                  25                  30          
Leu Val Val Arg Glu Ala Leu Ala Arg Ala Gln Val Ser Gly Asp Asp
        35                  40                  45              
Val Gly His Val Val Phe Gly Asn Val Ile Gln Thr Glu Pro Arg Asp
    50                  55                  60                  
Met Tyr Leu Gly Arg Val Ala Ala Val Asn Gly Gly Val Thr Ile Asn
65                  70                  75                  80  
Ala Pro Ala Leu Thr Val Asn Arg Leu Cys Gly Ser Gly Leu Gln Ala
                85                  90                  95      
Ile Val Ser Ala Ala Gln Thr Ile Leu Leu Gly Asp Thr Asp Val Ala
            100                 105                 110         
Ile Gly Gly Gly Ala Glu Ser Met Ser Arg Ala Pro Tyr Leu Ala Pro
        115                 120                 125             
Ala Ala Arg Trp Gly Ala Arg Met Gly Asp Ala Gly Leu Val Asp Met
    130                 135                 140                 
Met Leu Gly Ala Leu His Asp Pro Phe His Arg Ile His Met Gly Val
145                 150                 155                 160 
Thr Ala Glu Asn Val Ala Lys Glu Tyr Asp Ile Ser Arg Ala Gln Gln
                165                 170                 175     
Asp Glu Ala Ala Leu Glu Ser His Arg Arg Ala Ser Ala Ala Ile Lys
            180                 185                 190         
Ala Gly Tyr Phe Lys Asp Gln Ile Val Pro Val Val Ser Lys Gly Arg
        195                 200                 205             
Lys Gly Asp Val Thr Phe Asp Thr Asp Glu His Val Arg His Asp Ala
    210                 215                 220                 
Thr Ile Asp Asp Met Thr Lys Leu Arg Pro Val Phe Val Lys Glu Asn
225                 230                 235                 240 
Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Ala Ala Ala
                245                 250                 255     
Ala Val Val Met Met Glu Arg Ala Glu Ala Glu Arg Arg Gly Leu Lys
            260                 265                 270         
Pro Leu Ala Arg Leu Val Ser Tyr Gly His Ala Gly Val Asp Pro Lys
        275                 280                 285             
Ala Met Gly Ile Gly Pro Val Pro Ala Thr Lys Ile Ala Leu Glu Arg
    290                 295                 300                 
Ala Gly Leu Gln Val Ser Asp Leu Asp Val Ile Glu Ala Asn Glu Ala
305                 310                 315                 320 
Phe Ala Ala Gln Ala Cys Ala Val Thr Lys Ala Leu Gly Leu Asp Pro
                325                 330                 335     
Ala Lys Val Asn Pro Asn Gly Ser Gly Ile Ser Leu Gly His Pro Ile
            340                 345                 350         
Gly Ala Thr Gly Ala Leu Ile Thr Val Lys Ala Leu His Glu Leu Asn
        355                 360                 365             
Arg Val Gln Gly Arg Tyr Ala Leu Val Thr Met Cys Ile Gly Gly Gly
    370                 375                 380                 
Gln Gly Ile Ala Ala Ile Phe Glu Arg Ile
385                 390                 


<210> 2
<211> 401
<212> PRT
<213> Escherichia coli

<400> 2
Met Arg Glu Ala Phe Ile Cys Asp Gly Ile Arg Thr Pro Ile Gly Arg
 1               5                  10                  15      
Tyr Gly Gly Ala Leu Ser Ser Val Arg Ala Asp Asp Leu Ala Ala Ile
            20                  25                  30          
Pro Leu Arg Glu Leu Leu Val Arg Asn Pro Arg Leu Asp Ala Glu Cys
        35                  40                  45              
Ile Asp Asp Val Ile Leu Gly Cys Ala Asn Gln Ala Gly Glu Asp Asn
    50                  55                  60                  
Arg Asn Val Ala Arg Met Ala Thr Leu Leu Ala Gly Leu Pro Gln Ser
65                  70                  75                  80  
Val Ser Gly Thr Thr Ile Asn Arg Leu Cys Gly Ser Gly Leu Asp Ala
                85                  90                  95      
Leu Gly Phe Ala Ala Arg Ala Ile Lys Ala Gly Asp Gly Asp Leu Leu
            100                 105                 110         
Ile Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly
        115                 120                 125             
Lys Ala Ala Ser Ala Phe Ser Arg Gln Ala Glu Met Phe Asp Thr Thr
    130                 135                 140                 
Ile Gly Trp Arg Phe Val Asn Pro Leu Met Ala Gln Gln Phe Gly Thr
145                 150                 155                 160 
Asp Ser Met Pro Glu Thr Ala Glu Asn Val Ala Glu Leu Leu Lys Ile
                165                 170                 175     
Ser Arg Glu Asp Gln Asp Ser Phe Ala Leu Arg Ser Gln Gln Arg Thr
            180                 185                 190         
Ala Lys Ala Gln Ser Ser Gly Ile Leu Ala Glu Glu Ile Val Pro Val
        195                 200                 205             
Val Leu Lys Asn Lys Lys Gly Val Val Thr Glu Ile Gln His Asp Glu
    210                 215                 220                 
His Leu Arg Pro Glu Thr Thr Leu Glu Gln Leu Arg Gly Leu Lys Ala
225                 230                 235                 240 
Pro Phe Arg Ala Asn Gly Val Ile Thr Ala Gly Asn Ala Ser Gly Val
                245                 250                 255     
Asn Asp Gly Ala Ala Ala Leu Ile Ile Ala Ser Glu Gln Met Ala Ala
            260                 265                 270         
Ala Gln Gly Leu Thr Pro Arg Ala Arg Ile Val Ala Met Ala Thr Ala
        275                 280                 285             
Gly Val Glu Pro Arg Leu Met Gly Leu Gly Pro Val Pro Ala Thr Arg
    290                 295                 300                 
Arg Val Leu Glu Arg Ala Gly Leu Ser Ile His Asp Met Asp Val Ile
305                 310                 315                 320 
Glu Leu Asn Glu Ala Phe Ala Ala Gln Ala Leu Gly Val Leu Arg Glu
                325                 330                 335     
Leu Gly Leu Pro Asp Asp Ala Pro His Val Asn Pro Asn Gly Gly Ala
            340                 345                 350         
Ile Ala Leu Gly His Pro Leu Gly Met Ser Gly Ala Arg Leu Ala Leu
        355                 360                 365             
Ala Ala Ser His Glu Leu His Arg Arg Asn Gly Arg Tyr Ala Leu Cys
    370                 375                 380                 
Thr Met Cys Ile Gly Val Gly Gln Gly Ile Ala Met Ile Leu Glu Arg
385                 390                 395                 400 
Val
    


<210> 3
<211> 286
<212> PRT
<213> Escherichia coli

<400> 3
Met Ser Gln Ala Leu Lys Asn Leu Leu Thr Leu Leu Asn Leu Glu Lys
 1               5                  10                  15      
Ile Glu Glu Gly Leu Phe Arg Gly Gln Ser Glu Asp Leu Gly Leu Arg
            20                  25                  30          
Gln Val Phe Gly Gly Gln Val Val Gly Gln Ala Leu Tyr Ala Ala Lys
        35                  40                  45              
Glu Thr Val Pro Glu Glu Arg Leu Val His Ser Phe His Ser Tyr Phe
    50                  55                  60                  
Leu Arg Pro Gly Asp Ser Lys Lys Pro Ile Ile Tyr Asp Val Glu Thr
65                  70                  75                  80  
Leu Arg Asp Gly Asn Ser Phe Ser Ala Arg Arg Val Ala Ala Ile Gln
                85                  90                  95      
Asn Gly Lys Pro Ile Phe Tyr Met Thr Ala Ser Phe Gln Ala Pro Glu
            100                 105                 110         
Ala Gly Phe Glu His Gln Lys Thr Met Pro Ser Ala Pro Ala Pro Asp
        115                 120                 125             
Gly Leu Pro Ser Glu Thr Gln Ile Ala Gln Ser Leu Ala His Leu Leu
    130                 135                 140                 
Pro Pro Val Leu Lys Asp Lys Phe Ile Cys Asp Arg Pro Leu Glu Val
145                 150                 155                 160 
Arg Pro Val Glu Phe His Asn Pro Leu Lys Gly His Val Ala Glu Pro
                165                 170                 175     
His Arg Gln Val Trp Ile Arg Ala Asn Gly Ser Val Pro Asp Asp Leu
            180                 185                 190         
Arg Val His Gln Tyr Leu Leu Gly Tyr Ala Ser Asp Leu Asn Phe Leu
        195                 200                 205             
Pro Val Ala Leu Gln Pro His Gly Ile Gly Phe Leu Glu Pro Gly Ile
    210                 215                 220                 
Gln Ile Ala Thr Ile Asp His Ser Met Trp Phe His Arg Pro Phe Asn
225                 230                 235                 240 
Leu Asn Glu Trp Leu Leu Tyr Ser Val Glu Ser Thr Ser Ala Ser Ser
                245                 250                 255     
Ala Arg Gly Phe Val Arg Gly Glu Phe Tyr Thr Gln Asp Gly Val Leu
            260                 265                 270         
Val Ala Ser Thr Val Gln Glu Gly Val Met Arg Asn His Asn
        275                 280                 285     


<210> 4
<211> 310
<212> PRT
<213> Micrococcus luteus

<400> 4
Met Ser Glu Phe Thr Arg Phe Glu Gln Val Thr Val Leu Gly Thr Gly
 1               5                  10                  15      
Val Leu Gly Ser Gln Ile Ile Met Gln Ala Ala Tyr His Gly Lys Lys
            20                  25                  30          
Val Met Ala Tyr Asp Ala Val Pro Ala Ala Leu Glu Asn Leu Asp Lys
        35                  40                  45              
Arg Trp Ala Trp Ile Arg Gln Gly Tyr Glu Ala Asp Leu Gly Glu Gly
    50                  55                  60                  
Tyr Asp Ala Ala Arg Phe Asp Glu Ala Ile Ala Arg Ile Thr Pro Thr
65                  70                  75                  80  
Ser Asp Leu Ala Glu Ala Val Ala Asp Ala Asp Ile Val Ile Glu Ala
                85                  90                  95      
Val Pro Glu Asn Leu Glu Leu Lys Arg Lys Val Trp Ala Gln Val Gly
            100                 105                 110         
Glu Leu Ala Pro Ala Thr Thr Leu Phe Ala Thr Asn Thr Ser Ser Leu
        115                 120                 125             
Leu Pro Ser Asp Phe Ala Asp Ala Ser Gly His Pro Glu Arg Phe Leu
    130                 135                 140                 
Ala Leu His Tyr Ala Asn Arg Ile Trp Ala Gln Asn Thr Ala Glu Val
145                 150                 155                 160 
Met Gly Thr Ala Ala Thr Ser Pro Glu Ala Val Ala Gly Ala Leu Gln
                165                 170                 175     
Phe Ala Glu Glu Thr Gly Met Val Pro Val His Val Arg Lys Glu Ile
            180                 185                 190         
Pro Gly Tyr Phe Leu Asn Ser Leu Leu Ile Pro Trp Leu Gln Ala Gly
        195                 200                 205             
Ser Lys Leu Tyr Met His Gly Val Gly Asn Pro Ala Asp Ile Asp Arg
    210                 215                 220                 
Thr Trp Arg Val Ala Thr Gly Asn Glu Arg Gly Pro Phe Gln Thr Tyr
225                 230                 235                 240 
Asp Ile Val Gly Phe His Val Ala Ala Asn Val Ser Arg Asn Thr Gly
                245                 250                 255     
Val Asp Trp Gln Leu Gly Phe Ala Glu Met Leu Glu Lys Ser Ile Ala
            260                 265                 270         
Glu Gly His Ser Gly Val Ala Asp Gly Gln Gly Phe Tyr Arg Tyr Gly
        275                 280                 285             
Pro Asp Gly Glu Asn Leu Gly Pro Val Glu Asp Trp Asn Leu Gly Asp
    290                 295                 300                 
Lys Asp Thr Pro Leu Gly
305                 310 


<210> 5
<211> 436
<212> PRT
<213> Escherichia coli

<400> 5
Met Asp Ala Lys Gln Arg Ile Ala Arg Arg Val Ala Gln Glu Leu Arg
 1               5                  10                  15      
Asp Gly Asp Ile Val Asn Leu Gly Ile Gly Leu Pro Thr Met Val Ala
            20                  25                  30          
Asn Tyr Leu Pro Glu Gly Ile His Ile Thr Leu Gln Ser Glu Asn Gly
        35                  40                  45              
Phe Leu Gly Leu Gly Pro Val Thr Thr Ala His Pro Asp Leu Val Asn
    50                  55                  60                  
Ala Gly Gly Gln Pro Cys Gly Val Leu Pro Gly Ala Ala Met Phe Asp
65                  70                  75                  80  
Ser Ala Met Ser Phe Ala Leu Ile Arg Gly Gly His Ile Asp Ala Cys
                85                  90                  95      
Val Leu Gly Gly Leu Gln Val Asp Glu Glu Ala Asn Leu Ala Asn Trp
            100                 105                 110         
Val Val Pro Gly Lys Met Val Pro Gly Met Gly Gly Ala Met Asp Leu
        115                 120                 125             
Val Thr Gly Ser Arg Lys Val Ile Ile Ala Met Glu His Cys Ala Lys
    130                 135                 140                 
Asp Gly Ser Ala Lys Ile Leu Arg Arg Cys Thr Met Pro Leu Thr Ala
145                 150                 155                 160 
Gln His Ala Val His Met Leu Val Thr Glu Leu Ala Val Phe Arg Phe
                165                 170                 175     
Ile Asp Gly Lys Met Trp Leu Thr Glu Ile Ala Asp Gly Cys Asp Leu
            180                 185                 190         
Ala Thr Val Arg Ala Lys Thr Glu Ala Arg Phe Glu Val Ala Ala Asp
        195                 200                 205             
Leu Asn Thr Gln Arg Gly Asp Leu Met Lys Thr Lys Leu Met Thr Leu
    210                 215                 220                 
Gln Asp Ala Thr Gly Phe Phe Arg Asp Gly Met Thr Ile Met Val Gly
225                 230                 235                 240 
Gly Phe Met Gly Ile Gly Thr Pro Ser Arg Leu Val Glu Ala Leu Leu
                245                 250                 255     
Glu Ser Gly Val Arg Asp Leu Thr Leu Ile Ala Asn Asp Thr Ala Phe
            260                 265                 270         
Val Asp Thr Gly Ile Gly Pro Leu Ile Val Asn Gly Arg Val Arg Lys
        275                 280                 285             
Val Ile Ala Ser His Ile Gly Thr Asn Pro Glu Thr Gly Arg Arg Met
    290                 295                 300                 
Ile Ser Gly Glu Met Asp Val Val Leu Val Pro Gln Gly Thr Leu Ile
305                 310                 315                 320 
Glu Gln Ile Arg Cys Gly Gly Ala Gly Leu Gly Gly Phe Leu Thr Pro
                325                 330                 335     
Thr Gly Val Gly Thr Val Val Glu Glu Gly Lys Gln Thr Leu Thr Leu
            340                 345                 350         
Asp Gly Lys Thr Trp Leu Leu Glu Arg Pro Leu Arg Ala Asp Leu Ala
        355                 360                 365             
Leu Ile Arg Ala His Arg Cys Asp Thr Leu Gly Asn Leu Thr Tyr Gln
    370                 375                 380                 
Leu Ser Ala Arg Asn Phe Asn Pro Leu Ile Ala Leu Ala Ala Asp Ile
385                 390                 395                 400 
Thr Leu Val Glu Pro Asp Glu Leu Val Glu Thr Gly Glu Leu Gln Pro
                405                 410                 415     
Asp His Ile Val Thr Pro Gly Ala Val Ile Asp His Ile Ile Val Ser
            420                 425                 430         
Gln Glu Ser Lys
        435     


<210> 6
<211> 440
<212> PRT
<213> Pseudomonas putida

<400> 6
Met Ile Asn Lys Thr Tyr Glu Ser Ile Ala Ser Ala Val Glu Gly Ile
 1               5                  10                  15      
Thr Asp Gly Ser Thr Ile Met Val Gly Gly Phe Gly Thr Ala Gly Met
            20                  25                  30          
Pro Ser Glu Leu Ile Asp Ala Leu Ile Asp Thr Gly Thr Arg Asp Leu
        35                  40                  45              
Thr Ile Ile Ser Asn Asn Ala Gly Asn Gly Glu Ile Gly Leu Ala Ala
    50                  55                  60                  
Leu Leu Lys Ala Gly Ser Val Arg Lys Val Val Cys Ser Phe Pro Arg
65                  70                  75                  80  
Gln Ser Asp Ser Tyr Val Phe Asp Glu Leu Tyr Arg Ala Gly Lys Ile
                85                  90                  95      
Glu Leu Glu Val Val Pro Gln Gly Asn Leu Ala Glu Arg Ile Arg Ala
            100                 105                 110         
Ala Gly Ser Gly Ile Gly Ala Phe Phe Ser Pro Thr Gly Tyr Gly Thr
        115                 120                 125             
Leu Leu Ser Glu Gly Lys Glu Thr Arg Glu Ile Asp Gly Arg Gln Tyr
    130                 135                 140                 
Val Leu Glu Met Pro Leu His Ala Asp Phe Ala Leu Ile Lys Ala Tyr
145                 150                 155                 160 
Lys Gly Asp Arg Trp Gly Asn Leu Ile Tyr Arg Lys Ala Ala Arg Asn
                165                 170                 175     
Phe Gly Pro Ile Met Ala Met Ala Ala Lys Thr Ala Ile Ala Gln Val
            180                 185                 190         
Asp Gln Ile Val Glu Leu Gly Glu Leu Asp Pro Glu His Ile Ile Thr
        195                 200                 205             
Pro Gly Ile Phe Val Gln Arg Val Val Ala Val Thr Gly Ala Ala Ser
    210                 215                 220                 
Ser Ile Ala Met Thr Ile Thr Thr Lys Leu Ser Arg Thr Gln Met Ala
225                 230                 235                 240 
Gln Arg Val Ala Ala Asp Ile Gln Glu Gly Ala Tyr Val Asn Leu Gly
                245                 250                 255     
Ile Gly Ala Pro Thr Leu Val Ala Asn Phe Leu Gly Asp Lys Glu Val
            260                 265                 270         
Phe Leu His Ser Glu Asn Gly Leu Leu Gly Met Gly Pro Ser Pro Ala
        275                 280                 285             
Pro Gly Glu Glu Asp Asp Asp Leu Ile Asn Ala Gly Lys Gln His Val
    290                 295                 300                 
Thr Leu Leu Thr Gly Gly Ala Phe Phe His His Ala Asp Ser Phe Ser
305                 310                 315                 320 
Met Met Arg Gly Gly His Leu Asp Ile Ala Val Leu Gly Ala Phe Gln
                325                 330                 335     
Val Ser Val Lys Gly Asp Leu Ala Asn Trp His Thr Gly Ala Glu Gly
            340                 345                 350         
Ser Ile Pro Ala Val Gly Gly Ala Met Asp Leu Ala Thr Gly Ala Arg
        355                 360                 365             
Gln Val Phe Val Met Met Asp His Leu Thr Lys Ser Gly Glu Ser Lys
    370                 375                 380                 
Ile Val Pro Glu Cys Thr Tyr Pro Leu Thr Gly Ile Gly Cys Val Ser
385                 390                 395                 400 
Arg Ile Tyr Thr Asp Leu Ala Val Leu Glu Val Thr Ser Asp Gly Leu
                405                 410                 415     
Lys Val Val Glu Ile Cys Ala Asp Ile Asp Phe Asp Glu Leu Gln Lys
            420                 425                 430         
Leu Ser Gly Val Pro Leu Ile Lys
        435                 440 


<210> 7
<211> 524
<212> PRT
<213> Clostridium propionicum

<400> 7
Met Arg Lys Val Pro Ile Ile Thr Ala Asp Glu Ala Ala Lys Leu Ile
 1               5                  10                  15      
Lys Asp Gly Asp Thr Val Thr Thr Ser Gly Phe Val Gly Asn Ala Ile
            20                  25                  30          
Pro Glu Ala Leu Asp Arg Ala Val Glu Lys Arg Phe Leu Glu Thr Gly
        35                  40                  45              
Glu Pro Lys Asn Ile Thr Tyr Val Tyr Cys Gly Ser Gln Gly Asn Arg
    50                  55                  60                  
Asp Gly Arg Gly Ala Glu His Phe Ala His Glu Gly Leu Leu Lys Arg
65                  70                  75                  80  
Tyr Ile Ala Gly His Trp Ala Thr Val Pro Ala Leu Gly Lys Met Ala
                85                  90                  95      
Met Glu Asn Lys Met Glu Ala Tyr Asn Val Ser Gln Gly Ala Leu Cys
            100                 105                 110         
His Leu Phe Arg Asp Ile Ala Ser His Lys Pro Gly Val Phe Thr Lys
        115                 120                 125             
Val Gly Ile Gly Thr Phe Ile Asp Pro Arg Asn Gly Gly Gly Lys Val
    130                 135                 140                 
Asn Asp Ile Thr Lys Glu Asp Ile Val Glu Leu Val Glu Ile Lys Gly
145                 150                 155                 160 
Gln Glu Tyr Leu Phe Tyr Pro Ala Phe Pro Ile His Val Ala Leu Ile
                165                 170                 175     
Arg Gly Thr Tyr Ala Asp Glu Ser Gly Asn Ile Thr Phe Glu Lys Glu
            180                 185                 190         
Val Ala Pro Leu Glu Gly Thr Ser Val Cys Gln Ala Val Lys Asn Ser
        195                 200                 205             
Gly Gly Ile Val Val Val Gln Val Glu Arg Val Val Lys Ala Gly Thr
    210                 215                 220                 
Leu Asp Pro Arg His Val Lys Val Pro Gly Ile Tyr Val Asp Tyr Val
225                 230                 235                 240 
Val Val Ala Asp Pro Glu Asp His Gln Gln Ser Leu Asp Cys Glu Tyr
                245                 250                 255     
Asp Pro Ala Leu Ser Gly Glu His Arg Arg Pro Glu Val Val Gly Glu
            260                 265                 270         
Pro Leu Pro Leu Ser Ala Lys Lys Val Ile Gly Arg Arg Gly Ala Ile
        275                 280                 285             
Glu Leu Glu Lys Asp Val Ala Val Asn Leu Gly Val Gly Ala Pro Glu
    290                 295                 300                 
Tyr Val Ala Ser Val Ala Asp Glu Glu Gly Ile Val Asp Phe Met Thr
305                 310                 315                 320 
Leu Thr Ala Glu Ser Gly Ala Ile Gly Gly Val Pro Ala Gly Gly Val
                325                 330                 335     
Arg Phe Gly Ala Ser Tyr Asn Ala Asp Ala Leu Ile Asp Gln Gly Tyr
            340                 345                 350         
Gln Phe Asp Tyr Tyr Asp Gly Gly Gly Leu Asp Leu Cys Tyr Leu Gly
        355                 360                 365             
Leu Ala Glu Cys Asp Glu Lys Gly Asn Ile Asn Val Ser Arg Phe Gly
    370                 375                 380                 
Pro Arg Ile Ala Gly Cys Gly Gly Phe Ile Asn Ile Thr Gln Asn Thr
385                 390                 395                 400 
Pro Lys Val Phe Phe Cys Gly Thr Phe Thr Ala Gly Gly Leu Lys Val
                405                 410                 415     
Lys Ile Glu Asp Gly Lys Val Ile Ile Val Gln Glu Gly Lys Gln Lys
            420                 425                 430         
Lys Phe Leu Lys Ala Val Glu Gln Ile Thr Phe Asn Gly Asp Val Ala
        435                 440                 445             
Leu Ala Asn Lys Gln Gln Val Thr Tyr Ile Thr Glu Arg Cys Val Phe
    450                 455                 460                 
Leu Leu Lys Glu Asp Gly Leu His Leu Ser Glu Ile Ala Pro Gly Ile
465                 470                 475                 480 
Asp Leu Gln Thr Gln Ile Leu Asp Val Met Asp Phe Ala Pro Ile Ile
                485                 490                 495     
Asp Arg Asp Ala Asn Gly Gln Ile Lys Leu Met Asp Ala Ala Leu Phe
            500                 505                 510         
Ala Glu Gly Leu Met Gly Leu Lys Glu Met Lys Ser
        515                 520                 


<210> 8
<211> 246
<212> PRT
<213> Chromobacterium violaceum

<400> 8
Met Lys Gln Gln Glu Val Arg Gln Arg Ala Phe Ala Met Pro Leu Thr
 1               5                  10                  15      
Ser Pro Ala Phe Pro Pro Gly Pro Tyr Arg Phe Val Asn Arg Glu Tyr
            20                  25                  30          
Met Ile Ile Thr Tyr Arg Thr Asp Pro Ala Ala Ile Glu Ala Val Leu
        35                  40                  45              
Pro Glu Pro Leu Gln Met Ala Glu Pro Val Val Arg Tyr Glu Phe Ile
    50                  55                  60                  
Arg Met Pro Asp Ser Thr Gly Phe Gly Asp Tyr Ser Glu Ser Gly Gln
65                  70                  75                  80  
Val Ile Pro Val Thr Phe Arg Gly Glu Arg Gly Ser Tyr Thr Leu Ala
                85                  90                  95      
Met Phe Leu Asp Asp Gln Pro Pro Leu Ala Gly Gly Arg Glu Leu Trp
            100                 105                 110         
Gly Phe Pro Lys Lys Ala Gly Lys Pro Arg Leu Glu Val His Gln Asp
        115                 120                 125             
Thr Leu Val Gly Ser Leu Asp Phe Gly Pro Val Arg Ile Ala Thr Gly
    130                 135                 140                 
Thr Met Gly Tyr Lys Tyr Glu Ala Leu Asp Arg Ser Ala Leu Leu Ala
145                 150                 155                 160 
Ser Leu Ala Glu Pro Asn Phe Leu Leu Lys Ile Ile Pro His Val Asp
                165                 170                 175     
Gly Ser Pro Arg Ile Cys Glu Leu Val Arg Tyr His Thr Thr Asp Val
            180                 185                 190         
Ala Ile Lys Gly Ala Trp Ser Ala Pro Gly Ser Leu Glu Leu His Pro
        195                 200                 205             
His Ala Leu Ala Pro Val Ala Ala Leu Pro Val Leu Glu Val Leu Ser
    210                 215                 220                 
Ala Arg His Phe Val Cys Asp Leu Thr Leu Asp Leu Gly Thr Val Val
225                 230                 235                 240 
Phe Asp Tyr Leu Arg Gln
                245     


<210> 9
<211> 252
<212> PRT
<213> Lactobacillus brevis

<400> 9
Met Ser Asn Arg Leu Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Thr
 1               5                  10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Thr Lys Phe Val Glu Glu Gly Ala
            20                  25                  30          
Lys Val Met Ile Thr Gly Arg His Ser Asp Val Gly Glu Lys Ala Ala
        35                  40                  45              
Lys Ser Val Gly Thr Pro Asp Gln Ile Gln Phe Phe Gln His Asp Ser
    50                  55                  60                  
Ser Asp Glu Asp Gly Trp Thr Lys Leu Phe Asp Ala Thr Glu Lys Ala
65                  70                  75                  80  
Phe Gly Pro Val Ser Thr Leu Val Asn Asn Ala Gly Ile Ala Val Asn
                85                  90                  95      
Lys Ser Val Glu Glu Thr Thr Thr Ala Glu Trp Arg Lys Leu Leu Ala
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile
    130                 135                 140                 
Glu Gly Phe Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys
            180                 185                 190         
Thr Pro Leu Val Asp Asp Leu Pro Gly Ala Glu Glu Ala Met Ser Gln
        195                 200                 205             
Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala
    210                 215                 220                 
Tyr Ile Cys Val Tyr Leu Ala Ser Asn Glu Ser Lys Phe Ala Thr Gly
225                 230                 235                 240 
Ser Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln
                245                 250         


<210> 10
<211> 244
<212> PRT
<213> Clostridium acetobutylicum

<400> 10
Met Leu Lys Asp Glu Val Ile Lys Gln Ile Ser Thr Pro Leu Thr Ser
 1               5                  10                  15      
Pro Ala Phe Pro Arg Gly Pro Tyr Lys Phe His Asn Arg Glu Tyr Phe
            20                  25                  30          
Asn Ile Val Tyr Arg Thr Asp Met Asp Ala Leu Arg Lys Val Val Pro
        35                  40                  45              
Glu Pro Leu Glu Ile Asp Glu Pro Leu Val Arg Phe Glu Ile Met Ala
    50                  55                  60                  
Met His Asp Thr Ser Gly Leu Gly Cys Tyr Thr Glu Ser Gly Gln Ala
65                  70                  75                  80  
Ile Pro Val Ser Phe Asn Gly Val Lys Gly Asp Tyr Leu His Met Met
                85                  90                  95      
Tyr Leu Asp Asn Glu Pro Ala Ile Ala Val Gly Arg Glu Leu Ser Ala
            100                 105                 110         
Tyr Pro Lys Lys Leu Gly Tyr Pro Lys Leu Phe Val Asp Ser Asp Thr
        115                 120                 125             
Leu Val Gly Thr Leu Asp Tyr Gly Lys Leu Arg Val Ala Thr Ala Thr
    130                 135                 140                 
Met Gly Tyr Lys His Lys Ala Leu Asp Ala Asn Glu Ala Lys Asp Gln
145                 150                 155                 160 
Ile Cys Arg Pro Asn Tyr Met Leu Lys Ile Ile Pro Asn Tyr Asp Gly
                165                 170                 175     
Ser Pro Arg Ile Cys Glu Leu Ile Asn Ala Lys Ile Thr Asp Val Thr
            180                 185                 190         
Val His Glu Ala Trp Thr Gly Pro Thr Arg Leu Gln Leu Phe Asp His
        195                 200                 205             
Ala Met Ala Pro Leu Asn Asp Leu Pro Val Lys Glu Ile Val Ser Ser
    210                 215                 220                 
Ser His Ile Leu Ala Asp Ile Ile Leu Pro Arg Ala Glu Val Ile Tyr
225                 230                 235                 240 
Asp Tyr Leu Lys
                


<210> 11
<211> 351
<212> PRT
<213> Clostridium beijerinckii

<400> 11
Met Lys Gly Phe Ala Met Leu Gly Ile Asn Lys Leu Gly Trp Ile Glu
 1               5                  10                  15      
Lys Glu Arg Pro Val Ala Gly Ser Tyr Asp Ala Ile Val Arg Pro Leu
            20                  25                  30          
Ala Val Ser Pro Cys Thr Ser Asp Ile His Thr Val Phe Glu Gly Ala
        35                  40                  45              
Leu Gly Asp Arg Lys Asn Met Ile Leu Gly His Glu Ala Val Gly Glu
    50                  55                  60                  
Val Val Glu Val Gly Ser Glu Val Lys Asp Phe Lys Pro Gly Asp Arg
65                  70                  75                  80  
Val Ile Val Pro Cys Thr Thr Pro Asp Trp Arg Ser Leu Glu Val Gln
                85                  90                  95      
Ala Gly Phe Gln Gln His Ser Asn Gly Met Leu Ala Gly Trp Lys Phe
            100                 105                 110         
Ser Asn Phe Lys Asp Gly Val Phe Gly Glu Tyr Phe His Val Asn Asp
        115                 120                 125             
Ala Asp Met Asn Leu Ala Ile Leu Pro Lys Asp Met Pro Leu Glu Asn
    130                 135                 140                 
Ala Val Met Ile Thr Asp Met Met Thr Thr Gly Phe His Gly Ala Glu
145                 150                 155                 160 
Leu Ala Asp Ile Gln Met Gly Ser Ser Val Val Val Ile Gly Ile Gly
                165                 170                 175     
Ala Val Gly Leu Met Gly Ile Ala Gly Ala Lys Leu Arg Gly Ala Gly
            180                 185                 190         
Arg Ile Ile Gly Val Gly Ser Arg Pro Ile Cys Val Glu Ala Ala Lys
        195                 200                 205             
Phe Tyr Gly Ala Thr Asp Ile Leu Asn Tyr Lys Asn Gly His Ile Val
    210                 215                 220                 
Asp Gln Val Met Lys Leu Thr Asn Gly Lys Gly Val Asp Arg Val Ile
225                 230                 235                 240 
Met Ala Gly Gly Gly Ser Glu Thr Leu Ser Gln Ala Val Ser Met Val
                245                 250                 255     
Lys Pro Gly Gly Ile Ile Ser Asn Ile Asn Tyr His Gly Ser Gly Asp
            260                 265                 270         
Ala Leu Leu Ile Pro Arg Val Glu Trp Gly Cys Gly Met Ala His Lys
        275                 280                 285             
Thr Ile Lys Gly Gly Leu Cys Pro Gly Gly Arg Leu Arg Ala Glu Met
    290                 295                 300                 
Leu Arg Asp Met Val Val Tyr Asn Arg Val Asp Leu Ser Lys Leu Val
305                 310                 315                 320 
Thr His Val Tyr His Gly Phe Asp His Ile Glu Glu Ala Leu Leu Leu
                325                 330                 335     
Met Lys Asp Lys Pro Lys Asp Leu Ile Lys Ala Val Val Ile Leu
            340                 345                 350     


<210> 12
<211> 542
<212> PRT
<213> Cupriavidus necator

<400> 12
Met Lys Val Ile Thr Ala Arg Glu Ala Ala Ala Leu Val Gln Asp Gly
 1               5                  10                  15      
Trp Thr Val Ala Ser Ala Gly Phe Val Gly Ala Gly His Ala Glu Ala
            20                  25                  30          
Val Thr Glu Ala Leu Glu Gln Arg Phe Leu Gln Ser Gly Leu Pro Arg
        35                  40                  45              
Asp Leu Thr Leu Val Tyr Ser Ala Gly Gln Gly Asp Arg Gly Ala Arg
    50                  55                  60                  
Gly Val Asn His Phe Gly Asn Ala Gly Met Thr Ala Ser Ile Val Gly
65                  70                  75                  80  
Gly His Trp Arg Ser Ala Thr Arg Leu Ala Thr Leu Ala Met Ala Glu
                85                  90                  95      
Gln Cys Glu Gly Tyr Asn Leu Pro Gln Gly Val Leu Thr His Leu Tyr
            100                 105                 110         
Arg Ala Ile Ala Gly Gly Lys Pro Gly Val Met Thr Lys Ile Gly Leu
        115                 120                 125             
His Thr Phe Val Asp Pro Arg Thr Ala Gln Asp Ala Arg Tyr His Gly
    130                 135                 140                 
Gly Ala Val Asn Glu Arg Ala Arg Gln Ala Ile Ala Glu Gly Lys Ala
145                 150                 155                 160 
Cys Trp Val Asp Ala Val Asp Phe Arg Gly Asp Glu Tyr Leu Phe Tyr
                165                 170                 175     
Pro Ser Phe Pro Ile His Cys Ala Leu Ile Arg Cys Thr Ala Ala Asp
            180                 185                 190         
Ala Arg Gly Asn Leu Ser Thr His Arg Glu Ala Phe His His Glu Leu
        195                 200                 205             
Leu Ala Met Ala Gln Ala Ala His Asn Ser Gly Gly Ile Val Ile Ala
    210                 215                 220                 
Gln Val Glu Ser Leu Val Asp His His Glu Ile Leu Gln Ala Ile His
225                 230                 235                 240 
Val Pro Gly Ile Leu Val Asp Tyr Val Val Val Cys Asp Asn Pro Ala
                245                 250                 255     
Asn His Gln Met Thr Phe Ala Glu Ser Tyr Asn Pro Ala Tyr Val Thr
            260                 265                 270         
Pro Trp Gln Gly Glu Ala Ala Val Ala Glu Ala Glu Ala Ala Pro Val
        275                 280                 285             
Ala Ala Gly Pro Leu Asp Ala Arg Thr Ile Val Gln Arg Arg Ala Val
    290                 295                 300                 
Met Glu Leu Ala Arg Arg Ala Pro Arg Val Val Asn Leu Gly Val Gly
305                 310                 315                 320 
Met Pro Ala Ala Val Gly Met Leu Ala His Gln Ala Gly Leu Asp Gly
                325                 330                 335     
Phe Thr Leu Thr Val Glu Ala Gly Pro Ile Gly Gly Thr Pro Ala Asp
            340                 345                 350         
Gly Leu Ser Phe Gly Ala Ser Ala Tyr Pro Glu Ala Val Val Asp Gln
        355                 360                 365             
Pro Ala Gln Phe Asp Phe Tyr Glu Gly Gly Gly Ile Asp Leu Ala Ile
    370                 375                 380                 
Leu Gly Leu Ala Glu Leu Asp Gly His Gly Asn Val Asn Val Ser Lys
385                 390                 395                 400 
Phe Gly Glu Gly Glu Gly Ala Ser Ile Ala Gly Val Gly Gly Phe Ile
                405                 410                 415     
Asn Ile Thr Gln Ser Ala Arg Ala Val Val Phe Met Gly Thr Leu Thr
            420                 425                 430         
Ala Gly Gly Leu Glu Val Arg Ala Gly Asp Gly Gly Leu Gln Ile Val
        435                 440                 445             
Arg Glu Gly Arg Val Lys Lys Ile Val Pro Glu Val Ser His Leu Ser
    450                 455                 460                 
Phe Asn Gly Pro Tyr Val Ala Ser Leu Gly Ile Pro Val Leu Tyr Ile
465                 470                 475                 480 
Thr Glu Arg Ala Val Phe Glu Met Arg Ala Gly Ala Asp Gly Glu Ala
                485                 490                 495     
Arg Leu Thr Leu Val Glu Ile Ala Pro Gly Val Asp Leu Gln Arg Asp
            500                 505                 510         
Val Leu Asp Gln Cys Ser Thr Pro Ile Ala Val Ala Gln Asp Leu Arg
        515                 520                 525             
Glu Met Asp Ala Arg Leu Phe Gln Ala Gly Pro Leu His Leu
    530                 535                 540         


<210> 13
<211> 438
<212> PRT
<213> Clostridium aminobutyricum

<400> 13
Met Asp Trp Lys Lys Ile Tyr Glu Asp Arg Thr Cys Thr Ala Asp Glu
 1               5                  10                  15      
Ala Val Lys Ser Ile Lys Ser Gly Asp Arg Val Leu Phe Ala His Cys
            20                  25                  30          
Val Ala Glu Pro Pro Val Leu Val Glu Ala Met Val Ala Asn Ala Ala
        35                  40                  45              
Ala Tyr Lys Asn Val Thr Val Ser His Met Val Thr Leu Gly Lys Gly
    50                  55                  60                  
Glu Tyr Ser Lys Pro Glu Tyr Lys Glu Asn Phe Thr Phe Glu Gly Trp
65                  70                  75                  80  
Phe Thr Ser Pro Ser Thr Arg Gly Ser Ile Ala Glu Gly His Gly Gln
                85                  90                  95      
Phe Val Pro Val Phe Phe His Glu Val Pro Ser Leu Ile Arg Lys Asp
            100                 105                 110         
Ile Phe His Val Asp Val Phe Met Val Met Val Ser Pro Pro Asp His
        115                 120                 125             
Asn Gly Phe Cys Cys Val Gly Val Ser Ser Asp Tyr Thr Met Gln Ala
    130                 135                 140                 
Ile Lys Ser Ala Lys Ile Val Leu Ala Glu Val Asn Asp Gln Val Pro
145                 150                 155                 160 
Val Val Tyr Gly Asp Thr Phe Val His Val Ser Glu Ile Asp Lys Phe
                165                 170                 175     
Val Glu Thr Ser His Pro Leu Pro Glu Ile Gly Leu Pro Lys Ile Gly
            180                 185                 190         
Glu Val Glu Ala Ala Ile Gly Lys His Cys Ala Ser Leu Ile Glu Asp
        195                 200                 205             
Gly Ser Thr Leu Gln Leu Gly Ile Gly Ala Ile Pro Asp Ala Val Leu
    210                 215                 220                 
Ser Gln Leu Lys Asp Lys Lys His Leu Gly Ile His Ser Glu Met Ile
225                 230                 235                 240 
Ser Asp Gly Val Val Asp Leu Tyr Glu Ala Gly Val Ile Asp Cys Ser
                245                 250                 255     
Gln Lys Ser Ile Asp Lys Gly Lys Met Ala Ile Thr Phe Leu Met Gly
            260                 265                 270         
Thr Lys Arg Leu Tyr Asp Phe Ala Ala Asn Asn Pro Lys Val Glu Leu
        275                 280                 285             
Lys Pro Val Asp Tyr Ile Asn His Pro Ser Val Val Ala Gln Cys Ser
    290                 295                 300                 
Lys Met Val Cys Ile Asn Ala Cys Leu Gln Val Asp Phe Met Gly Gln
305                 310                 315                 320 
Ile Val Ser Asp Ser Ile Gly Thr Lys Gln Phe Ser Gly Val Gly Gly
                325                 330                 335     
Gln Val Asp Phe Val Arg Gly Ala Ser Met Ser Ile Asp Gly Lys Gly
            340                 345                 350         
Lys Ala Ile Ile Ala Met Pro Ser Val Ala Lys Lys Lys Asp Gly Ser
        355                 360                 365             
Met Ile Ser Lys Ile Val Pro Phe Ile Asp His Gly Ala Ala Val Thr
    370                 375                 380                 
Thr Ser Arg Asn Asp Ala Asp Tyr Val Val Thr Glu Tyr Gly Ile Ala
385                 390                 395                 400 
Glu Met Lys Gly Lys Ser Leu Gln Asp Arg Ala Arg Ala Leu Ile Asn
                405                 410                 415     
Ile Ala His Pro Asp Phe Lys Asp Glu Leu Lys Ala Glu Phe Glu Lys
            420                 425                 430         
Arg Phe Asn Ala Ala Phe
        435             


<210> 14
<211> 132
<212> PRT
<213> Escherichia coli

<400> 14
Met Ser Thr Thr His Asn Val Pro Gln Gly Asp Leu Val Leu Arg Thr
 1               5                  10                  15      
Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly
            20                  25                  30          
Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu
        35                  40                  45              
Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe
    50                  55                  60                  
Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys
65                  70                  75                  80  
Val Gln Lys Gly Thr Thr Ser Val Ser Ile Asn Ile Glu Val Trp Val
                85                  90                  95      
Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu
            100                 105                 110         
Ala Leu Phe Lys Tyr Val Ala Val Asp Pro Glu Gly Lys Pro Arg Ala
        115                 120                 125             
Leu Pro Val Glu
    130         

