                         SEQUENCE LISTING

<110>  COUNCIL OF SCIENTIFIC & INDUSTRIAL REASEARCH
 
<120>  A PRIMER SEQUENCE FOR THE PREPARATION OF INSECTICIDAL PROTEIN 
       FROM MICROSPORIUM  SCOLOPENDRIUM

<130>  0143NF2012

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1062
<212>  DNA
<213>  artificial sequence

<220>
<223>  insecticial protein cDNA

<400>  1
atggcgctgt accagacacc cgtgtatgtc atcggagggc aaggtggcaa ctcgtttact       60

tatgaccaga gcaggaacgg gaaggtgttg aggaagattg gggtgtgggc tggcgagtgg      120

cagctgcgcg gcatccgggt atggatgtct ggctccgata cgccggctac ctttggctca      180

gcctcgggct cttattctga gtatacattt gcggatggcg agcgcatcac ccgcttgtcc      240

ttgtggggca acggtgctgg tacgcgttcc ggagctatta gattctacac gacaactgga      300

ggcacatttt tcccaaaaat gacatcttgg ggcttacaga ctgagtatcc aattgatgtg      360

gcatctggtc tttgtgtggg aatcttagga cgagctagtg ctgatgttga ttcattgggg      420

ttcctatttc tccgaaccat agcatcggct cgtatgatca atgtaagcta cccaaccttg      480

ggcttggagc aagctggaat tgtccctgtc acgcttgatt cgtacaacga ctccaataat      540

tcaggttcta tctccaagaa ttggactttc tctggtagtc gaacagttac aatatcatca      600

tcatggacgc ttactacagg gatagaggca catgctactt tgactgttca agcagggatc      660

ccttcagttg cagaagttac tggagagttt ggatgggcag tgagtgtgac cggaagccac      720

acaaccaccg aagaggaaag ccgaacgctc cgttgggagc aatccggaac cttagagcca      780

ggacaatgga tttctctcca agctaccact cgaagaggaa ccatcacctt accctaccaa      840

gggactatgg agatcaccct acagtctggc accgtgtttc aatatcccat atcctctatg      900

tattctggtg ttgattatac tagtgttgac ataatcaaca ctggaactag agcattggat      960

caagctaagg tggaagatat tgatcaaaaa tcccaagaaa aggatcacaa tgcccaacct     1020

aatgaacaag tccaagaaag caaactcctc tttgttgagt aa                        1062


<210>  2
<211>  353
<212>  PRT
<213>  artificial sequence

<220>
<223>  insecticidal protein

<400>  2

Met Ala Leu Tyr Gln Thr Pro Val Tyr Val Ile Gly Gly Gln Gly Gly 
1               5                   10                  15      


Asn Ser Phe Thr Tyr Asp Gln Ser Arg Asn Gly Lys Val Leu Arg Lys 
            20                  25                  30          


Ile Gly Val Trp Ala Gly Glu Trp Gln Leu Arg Gly Ile Arg Val Trp 
        35                  40                  45              


Met Ser Gly Ser Asp Thr Pro Ala Thr Phe Gly Ser Ala Ser Gly Ser 
    50                  55                  60                  


Tyr Ser Glu Tyr Thr Phe Ala Asp Gly Glu Arg Ile Thr Arg Leu Ser 
65                  70                  75                  80  


Leu Trp Gly Asn Gly Ala Gly Thr Arg Ser Gly Ala Ile Arg Phe Tyr 
                85                  90                  95      


Thr Thr Thr Gly Gly Thr Phe Phe Pro Lys Met Thr Ser Trp Gly Leu 
            100                 105                 110         


Gln Thr Glu Tyr Pro Ile Asp Val Ala Ser Gly Leu Cys Val Gly Ile 
        115                 120                 125             


Leu Gly Arg Ala Ser Ala Asp Val Asp Ser Leu Gly Phe Leu Phe Leu 
    130                 135                 140                 


Arg Thr Ile Ala Ser Ala Arg Met Ile Asn Val Ser Tyr Pro Thr Leu 
145                 150                 155                 160 


Gly Leu Glu Gln Ala Gly Ile Val Pro Val Thr Leu Asp Ser Tyr Asn 
                165                 170                 175     


Asp Ser Asn Asn Ser Gly Ser Ile Ser Lys Asn Trp Thr Phe Ser Gly 
            180                 185                 190         


Ser Arg Thr Val Thr Ile Ser Ser Ser Trp Thr Leu Thr Thr Gly Ile 
        195                 200                 205             


Glu Ala His Ala Thr Leu Thr Val Gln Ala Gly Ile Pro Ser Val Ala 
    210                 215                 220                 


Glu Val Thr Gly Glu Phe Gly Trp Ala Val Ser Val Thr Gly Ser His 
225                 230                 235                 240 


Thr Thr Thr Glu Glu Glu Ser Arg Thr Leu Arg Trp Glu Gln Ser Gly 
                245                 250                 255     


Thr Leu Glu Pro Gly Gln Trp Ile Ser Leu Gln Ala Thr Thr Arg Arg 
            260                 265                 270         


Gly Thr Ile Thr Leu Pro Tyr Gln Gly Thr Met Glu Ile Thr Leu Gln 
        275                 280                 285             


Ser Gly Thr Val Phe Gln Tyr Pro Ile Ser Ser Met Tyr Ser Gly Val 
    290                 295                 300                 


Asp Tyr Thr Ser Val Asp Ile Ile Asn Thr Gly Thr Arg Ala Leu Asp 
305                 310                 315                 320 


Gln Ala Lys Val Glu Asp Ile Asp Gln Lys Ser Gln Glu Lys Asp His 
                325                 330                 335     


Asn Ala Gln Pro Asn Glu Gln Val Gln Glu Ser Lys Leu Leu Phe Val 
            340                 345                 350         


Glu 
    


<210>  3
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer  (GSP1) sequence for amplifying DNA encoding insecticidal 
       protein

<400>  3
atggcgctgt accagacacc cgtgtatg                                          28


<210>  4
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer  (GSP2) sequence for amplifying DNA encoding insecticidal 
       protein

<400>  4
ttactcaaca aagaggagtt tgcttcttgg                                        30


