                         SEQUENCE LISTING

<110>  Evolva SA
 
<120>  METHODS AND MATERIALS FOR BIOSYNTHESIS OF MANOYL OXIDE

<130>  KER/546135WO

<150>  PA 201470694
<151>  2014-11-13

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  894
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding GGPPS7 of Synechococcus - codon optimized for 
       expression in S. cerevisiae

<400>  1
atggtcgcac aaactttcaa cctggatacc tacttatccc aaagacaaca acaagttgaa       60

gaggccctaa gtgctgctct tgtgccagct tatcctgaga gaatatacga agctatgaga      120

tactccctcc tggcaggtgg caaaagatta agacctatct tatgtttagc tgcttgcgaa      180

ttggcaggtg gttctgttga acaagccatg ccaactgcgt gtgcacttga aatgatccat      240

acaatgtcac taattcatga tgacctgcca gccatggata acgatgattt cagaagagga      300

aagccaacta atcacaaggt gttcggggaa gatatagcca tcttagcggg tgatgcgctt      360

ttagcttacg cttttgaaca tattgcttct caaacaagag gagtaccacc tcaattggtg      420

ctacaagtta ttgctagaat cggacacgcc gttgctgcaa caggcctcgt tggaggccaa      480

gtcgtagacc ttgaatctga aggtaaagct atttccttag aaacattgga gtatattcac      540

tcacataaga ctggagcctt gctggaagca tcagttgtct caggcggtat tctcgcaggg      600

gcagatgaag agcttttggc cagattgtct cattacgcta gagatatagg cttggctttt      660

caaatcgtcg atgatatcct ggatgttact gctacatctg aacagttggg gaaaaccgct      720

ggtaaagacc aggcagccgc aaaggcaact tatccaagtc tattgggttt agaagcctct      780

agacagaaag cggaagagtt gattcaatct gctaaggaag ccttaagacc ttacggttca      840

caagcagagc cactcctagc gctggcagac ttcatcacac gtcgtcagca ttaa            894


<210>  2
<211>  297
<212>  PRT
<213>  Synechococcus

<400>  2

Met Val Ala Gln Thr Phe Asn Leu Asp Thr Tyr Leu Ser Gln Arg Gln 
1               5                   10                  15      


Gln Gln Val Glu Glu Ala Leu Ser Ala Ala Leu Val Pro Ala Tyr Pro 
            20                  25                  30          


Glu Arg Ile Tyr Glu Ala Met Arg Tyr Ser Leu Leu Ala Gly Gly Lys 
        35                  40                  45              


Arg Leu Arg Pro Ile Leu Cys Leu Ala Ala Cys Glu Leu Ala Gly Gly 
    50                  55                  60                  


Ser Val Glu Gln Ala Met Pro Thr Ala Cys Ala Leu Glu Met Ile His 
65                  70                  75                  80  


Thr Met Ser Leu Ile His Asp Asp Leu Pro Ala Met Asp Asn Asp Asp 
                85                  90                  95      


Phe Arg Arg Gly Lys Pro Thr Asn His Lys Val Phe Gly Glu Asp Ile 
            100                 105                 110         


Ala Ile Leu Ala Gly Asp Ala Leu Leu Ala Tyr Ala Phe Glu His Ile 
        115                 120                 125             


Ala Ser Gln Thr Arg Gly Val Pro Pro Gln Leu Val Leu Gln Val Ile 
    130                 135                 140                 


Ala Arg Ile Gly His Ala Val Ala Ala Thr Gly Leu Val Gly Gly Gln 
145                 150                 155                 160 


Val Val Asp Leu Glu Ser Glu Gly Lys Ala Ile Ser Leu Glu Thr Leu 
                165                 170                 175     


Glu Tyr Ile His Ser His Lys Thr Gly Ala Leu Leu Glu Ala Ser Val 
            180                 185                 190         


Val Ser Gly Gly Ile Leu Ala Gly Ala Asp Glu Glu Leu Leu Ala Arg 
        195                 200                 205             


Leu Ser His Tyr Ala Arg Asp Ile Gly Leu Ala Phe Gln Ile Val Asp 
    210                 215                 220                 


Asp Ile Leu Asp Val Thr Ala Thr Ser Glu Gln Leu Gly Lys Thr Ala 
225                 230                 235                 240 


Gly Lys Asp Gln Ala Ala Ala Lys Ala Thr Tyr Pro Ser Leu Leu Gly 
                245                 250                 255     


Leu Glu Ala Ser Arg Gln Lys Ala Glu Glu Leu Ile Gln Ser Ala Lys 
            260                 265                 270         


Glu Ala Leu Arg Pro Tyr Gly Ser Gln Ala Glu Pro Leu Leu Ala Leu 
        275                 280                 285             


Ala Asp Phe Ile Thr Arg Arg Gln His 
    290                 295         


<210>  3
<211>  1191
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding GGPPS10 of Aspergillus nidulans - codon 
       optimized for expression in S. cerevisiae

<400>  3
atgacgtccg attcgcactt tcaccctccg cacgcaatac cacccaggat tagctccaac       60

cgaatgtcag gtgcatctac tcgagacaag gcagcactaa tgggaaactt cgagaaggac      120

tggctgtcaa aaggtgacaa gcttcagaca aacaccgatt tgtctaaaag acacacgcga      180

aatcagtcaa gtctcgacgg gacaaaatac aaagatggga aatggtccca agagaatgag      240

gaggtgatca tgggtccgta cgactacatg ctgcaacacc cggggaagga cctgcgacgg      300

cagatgatca acgcttttaa cgtatggttg aaggtgccat ctgagagcct ggccatcatc      360

accaaagtag tggctatgct ccataccgct tcattattga tcgacgacgt cgaagacaac      420

tctcttctcc ggcgaggaat tccggtcgca catagcatct atggcaccgc gcagacgatc      480

aattcggcaa actacgttta cttcctcgcc ctccaggagg tgcaaaaact gaagagtccg      540

gcagctatcg acatatacgt ccaggagctg ctgaatttac acagagggca aggcatggat      600

ctgttctggc gagacacgct cacttgtcca agcgaagatg aatacttgga gatggtgggc      660

aacaagactg gaggtttgtt ccggctagct gtgaaattga tgcaagctga aagcagcact      720

ggaaaggact gtgtggccct tgtgaatgtt ttgggactgg tctttcagat atgcgacgac      780

tatctcaatt tatccgacac gacgtatacc cagaacaaag ggctctgtga agacctcaca      840

gagggcaaat tttcattccc cattatccac agcattcgat cgaacccggg gaaccatcag      900

ctcatcaata tcctccggca gagaacaaag gatgaagaag tcaaacgcta cgcgctccag      960

tatatggaaa gcacgggcag tttcaagcat acgcaggatg ttgttcggca gctacgtgcc     1020

agagctctgc agctcattga agagattgag aacagcgaaa atggcgagca accggaggaa     1080

cacaatgacg gtacgatggt ccgggcaatc ctcgataaaa tcacagaatc caccttggct     1140

gatacgaata cgactacgag agatatcaac ggcaactgtg cgacccgtta a              1191


<210>  4
<211>  396
<212>  PRT
<213>  Aspergillus nidulans

<400>  4

Met Thr Ser Asp Ser His Phe His Pro Pro His Ala Ile Pro Pro Arg 
1               5                   10                  15      


Ile Ser Ser Asn Arg Met Ser Gly Ala Ser Thr Arg Asp Lys Ala Ala 
            20                  25                  30          


Leu Met Gly Asn Phe Glu Lys Asp Trp Leu Ser Lys Gly Asp Lys Leu 
        35                  40                  45              


Gln Thr Asn Thr Asp Leu Ser Lys Arg His Thr Arg Asn Gln Ser Ser 
    50                  55                  60                  


Leu Asp Gly Thr Lys Tyr Lys Asp Gly Lys Trp Ser Gln Glu Asn Glu 
65                  70                  75                  80  


Glu Val Ile Met Gly Pro Tyr Asp Tyr Met Leu Gln His Pro Gly Lys 
                85                  90                  95      


Asp Leu Arg Arg Gln Met Ile Asn Ala Phe Asn Val Trp Leu Lys Val 
            100                 105                 110         


Pro Ser Glu Ser Leu Ala Ile Ile Thr Lys Val Val Ala Met Leu His 
        115                 120                 125             


Thr Ala Ser Leu Leu Ile Asp Asp Val Glu Asp Asn Ser Leu Leu Arg 
    130                 135                 140                 


Arg Gly Ile Pro Val Ala His Ser Ile Tyr Gly Thr Ala Gln Thr Ile 
145                 150                 155                 160 


Asn Ser Ala Asn Tyr Val Tyr Phe Leu Ala Leu Gln Glu Val Gln Lys 
                165                 170                 175     


Leu Lys Ser Pro Ala Ala Ile Asp Ile Tyr Val Gln Glu Leu Leu Asn 
            180                 185                 190         


Leu His Arg Gly Gln Gly Met Asp Leu Phe Trp Arg Asp Thr Leu Thr 
        195                 200                 205             


Cys Pro Ser Glu Asp Glu Tyr Leu Glu Met Val Gly Asn Lys Thr Gly 
    210                 215                 220                 


Gly Leu Phe Arg Leu Ala Val Lys Leu Met Gln Ala Glu Ser Ser Thr 
225                 230                 235                 240 


Gly Lys Asp Cys Val Ala Leu Val Asn Val Leu Gly Leu Val Phe Gln 
                245                 250                 255     


Ile Cys Asp Asp Tyr Leu Asn Leu Ser Asp Thr Thr Tyr Thr Gln Asn 
            260                 265                 270         


Lys Gly Leu Cys Glu Asp Leu Thr Glu Gly Lys Phe Ser Phe Pro Ile 
        275                 280                 285             


Ile His Ser Ile Arg Ser Asn Pro Gly Asn His Gln Leu Ile Asn Ile 
    290                 295                 300                 


Leu Arg Gln Arg Thr Lys Asp Glu Glu Val Lys Arg Tyr Ala Leu Gln 
305                 310                 315                 320 


Tyr Met Glu Ser Thr Gly Ser Phe Lys His Thr Gln Asp Val Val Arg 
                325                 330                 335     


Gln Leu Arg Ala Arg Ala Leu Gln Leu Ile Glu Glu Ile Glu Asn Ser 
            340                 345                 350         


Glu Asn Gly Glu Gln Pro Glu Glu His Asn Asp Gly Thr Met Val Arg 
        355                 360                 365             


Ala Ile Leu Asp Lys Ile Thr Glu Ser Thr Leu Ala Asp Thr Asn Thr 
    370                 375                 380                 


Thr Thr Arg Asp Ile Asn Gly Asn Cys Ala Thr Arg 
385                 390                 395     


<210>  5
<211>  2196
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cDNA encoding Coleus forskohlii TPS2

<400>  5
atgagtcgag ttgcaagtct ggatgcgttg aatggaattc aaaaagtcgg cccagccacc       60

attgggactc ctgaagagga aaataaaaag attgaggatt ccattgagta cgtgaaggag      120

ttgttgaaga caatgggcga cgggcgaatc agcgtttccc cgtacgacac agcaatagtt      180

gccctgatta aggacttgga aggaggtgat ggaccagagt ttccatcttg tctagagtgg      240

attgcacaga atcaactggc tgatggttct tggggggatc acttcttctg tatttatgat      300

cgggttgtta atacagcagc ttgtgtggtc gccttaaagt cgtggaacgt tcacgcagac      360

aagattgaga aaggagcagt gtacctgaag gagaatgtgc ataaacttaa agatgggaag      420

attgagcaca tgcccgcagg gtttgaattt gtggttcctg ccactcttga aagagccaaa      480

gccttgggga tcaaaggtct tccctatgat gatcctttca tcagggaaat ttatagtgca      540

aaacaaacaa gattgaccaa gataccaaag ggcatgatct acgaatctcc aacttcttta      600

ttatatagtt tagacggtct ggaaggcttg gagtgggaca agatactgaa actgcagtcg      660

gccgatggct cattcatcac ctctgtgtcg tctactgcct tcgtattcat gcacaccaac      720

gaccttaaat gccacgcctt catcaaaaat gccctcacca attgcaacgg gggagtaccc      780

cacacgtatc cagtggatat cttcgcacga ctttgggcag tggaccgact gcaacgcctc      840

ggaatatctc gattctttga gcctgagatc aaatatttaa tggatcacat caataacgtg      900

tggagggaga agggagtttt cagttcaagg cattcacaat ttgcggatat tgacgacaca      960

tccatgggca tcaggcttct gaaaatgcac ggatacaatg tcaacccaaa tgcacttgaa     1020

catttcaaac agaaagatgg gaagtttaca tgctatgctg atcaacatat cgagtctcca     1080

tcccccatgt acaatctcta cagggctgct cagcttcgtt ttccaggaga agaaattctt     1140

caacaagccc ttcaatttgc ctataatttt ctacatgaaa acctagccag caatcacttt     1200

caagaaaaat gggtcatatc cgaccaccta attgatgagg taaggatcgg gctgaagatg     1260

ccatggtacg ccaccctacc gcgagtggag gcttcatact atcttcaaca ttatggtgga     1320

tccagcgacg tatggattgg caaaacttta tacagaatgc cagaaatcag taacgacaca     1380

tacaaaatac ttgcacaatt ggacttcaac aaatgtcaag cacaacatca gttggaatgg     1440

atgtccatga aagagtggta tcaaagtaat aatgttaaag aatttgggat aagcaagaaa     1500

gaacttcttc ttgcttactt tttggctgct gcaaccatgt ttgaacccga acgcacacaa     1560

gagaggatta tgtgggcgaa aactcaagtc gtttctcgga tgatcacatc atttctcaac     1620

aaagaaaaca caatgtcatt cgacctaaag attgcacttt taacccaacc ccaacatcaa     1680

ataaatggtt ctgagatgaa gaatggactt gctcaaactc ttcctgcagc cttccgacaa     1740

ctactcaagg aattcgacaa atacacaaga catcaattga ggaatacttg gaacaaatgg     1800

ttgatgaaac tgaagcaagg agatgacaat ggcggcgcag atgcagagct ccttgcaaac     1860

acattaaaca tatgtgctgg acataacgaa gacatattat cgcactatga atacaccgct     1920

ctctcctccc tcacaaacaa aatatgtcag cgtctaagtc aaattcaaga taaaaagatg     1980

ctggaaattg aggaggggag cataaaagat aaggagatgg agctcgaaat acaaacattg     2040

gtgaagttag tcctccaaga aaccagtggg ggtatcgata gaaacatcaa gcaaacattt     2100

ttatcagtat tcaagacatt ttactacagg gcctaccacg atgctaagac tatcgatgcc     2160

catattttcc aagtactatt tgaaccagtg gtctga                               2196


<210>  6
<211>  731
<212>  PRT
<213>  Coleus forskohlii

<400>  6

Met Ser Arg Val Ala Ser Leu Asp Ala Leu Asn Gly Ile Gln Lys Val 
1               5                   10                  15      


Gly Pro Ala Thr Ile Gly Thr Pro Glu Glu Glu Asn Lys Lys Ile Glu 
            20                  25                  30          


Asp Ser Ile Glu Tyr Val Lys Glu Leu Leu Lys Thr Met Gly Asp Gly 
        35                  40                  45              


Arg Ile Ser Val Ser Pro Tyr Asp Thr Ala Ile Val Ala Leu Ile Lys 
    50                  55                  60                  


Asp Leu Glu Gly Gly Asp Gly Pro Glu Phe Pro Ser Cys Leu Glu Trp 
65                  70                  75                  80  


Ile Ala Gln Asn Gln Leu Ala Asp Gly Ser Trp Gly Asp His Phe Phe 
                85                  90                  95      


Cys Ile Tyr Asp Arg Val Val Asn Thr Ala Ala Cys Val Val Ala Leu 
            100                 105                 110         


Lys Ser Trp Asn Val His Ala Asp Lys Ile Glu Lys Gly Ala Val Tyr 
        115                 120                 125             


Leu Lys Glu Asn Val His Lys Leu Lys Asp Gly Lys Ile Glu His Met 
    130                 135                 140                 


Pro Ala Gly Phe Glu Phe Val Val Pro Ala Thr Leu Glu Arg Ala Lys 
145                 150                 155                 160 


Ala Leu Gly Ile Lys Gly Leu Pro Tyr Asp Asp Pro Phe Ile Arg Glu 
                165                 170                 175     


Ile Tyr Ser Ala Lys Gln Thr Arg Leu Thr Lys Ile Pro Lys Gly Met 
            180                 185                 190         


Ile Tyr Glu Ser Pro Thr Ser Leu Leu Tyr Ser Leu Asp Gly Leu Glu 
        195                 200                 205             


Gly Leu Glu Trp Asp Lys Ile Leu Lys Leu Gln Ser Ala Asp Gly Ser 
    210                 215                 220                 


Phe Ile Thr Ser Val Ser Ser Thr Ala Phe Val Phe Met His Thr Asn 
225                 230                 235                 240 


Asp Leu Lys Cys His Ala Phe Ile Lys Asn Ala Leu Thr Asn Cys Asn 
                245                 250                 255     


Gly Gly Val Pro His Thr Tyr Pro Val Asp Ile Phe Ala Arg Leu Trp 
            260                 265                 270         


Ala Val Asp Arg Leu Gln Arg Leu Gly Ile Ser Arg Phe Phe Glu Pro 
        275                 280                 285             


Glu Ile Lys Tyr Leu Met Asp His Ile Asn Asn Val Trp Arg Glu Lys 
    290                 295                 300                 


Gly Val Phe Ser Ser Arg His Ser Gln Phe Ala Asp Ile Asp Asp Thr 
305                 310                 315                 320 


Ser Met Gly Ile Arg Leu Leu Lys Met His Gly Tyr Asn Val Asn Pro 
                325                 330                 335     


Asn Ala Leu Glu His Phe Lys Gln Lys Asp Gly Lys Phe Thr Cys Tyr 
            340                 345                 350         


Ala Asp Gln His Ile Glu Ser Pro Ser Pro Met Tyr Asn Leu Tyr Arg 
        355                 360                 365             


Ala Ala Gln Leu Arg Phe Pro Gly Glu Glu Ile Leu Gln Gln Ala Leu 
    370                 375                 380                 


Gln Phe Ala Tyr Asn Phe Leu His Glu Asn Leu Ala Ser Asn His Phe 
385                 390                 395                 400 


Gln Glu Lys Trp Val Ile Ser Asp His Leu Ile Asp Glu Val Arg Ile 
                405                 410                 415     


Gly Leu Lys Met Pro Trp Tyr Ala Thr Leu Pro Arg Val Glu Ala Ser 
            420                 425                 430         


Tyr Tyr Leu Gln His Tyr Gly Gly Ser Ser Asp Val Trp Ile Gly Lys 
        435                 440                 445             


Thr Leu Tyr Arg Met Pro Glu Ile Ser Asn Asp Thr Tyr Lys Ile Leu 
    450                 455                 460                 


Ala Gln Leu Asp Phe Asn Lys Cys Gln Ala Gln His Gln Leu Glu Trp 
465                 470                 475                 480 


Met Ser Met Lys Glu Trp Tyr Gln Ser Asn Asn Val Lys Glu Phe Gly 
                485                 490                 495     


Ile Ser Lys Lys Glu Leu Leu Leu Ala Tyr Phe Leu Ala Ala Ala Thr 
            500                 505                 510         


Met Phe Glu Pro Glu Arg Thr Gln Glu Arg Ile Met Trp Ala Lys Thr 
        515                 520                 525             


Gln Val Val Ser Arg Met Ile Thr Ser Phe Leu Asn Lys Glu Asn Thr 
    530                 535                 540                 


Met Ser Phe Asp Leu Lys Ile Ala Leu Leu Thr Gln Pro Gln His Gln 
545                 550                 555                 560 


Ile Asn Gly Ser Glu Met Lys Asn Gly Leu Ala Gln Thr Leu Pro Ala 
                565                 570                 575     


Ala Phe Arg Gln Leu Leu Lys Glu Phe Asp Lys Tyr Thr Arg His Gln 
            580                 585                 590         


Leu Arg Asn Thr Trp Asn Lys Trp Leu Met Lys Leu Lys Gln Gly Asp 
        595                 600                 605             


Asp Asn Gly Gly Ala Asp Ala Glu Leu Leu Ala Asn Thr Leu Asn Ile 
    610                 615                 620                 


Cys Ala Gly His Asn Glu Asp Ile Leu Ser His Tyr Glu Tyr Thr Ala 
625                 630                 635                 640 


Leu Ser Ser Leu Thr Asn Lys Ile Cys Gln Arg Leu Ser Gln Ile Gln 
                645                 650                 655     


Asp Lys Lys Met Leu Glu Ile Glu Glu Gly Ser Ile Lys Asp Lys Glu 
            660                 665                 670         


Met Glu Leu Glu Ile Gln Thr Leu Val Lys Leu Val Leu Gln Glu Thr 
        675                 680                 685             


Ser Gly Gly Ile Asp Arg Asn Ile Lys Gln Thr Phe Leu Ser Val Phe 
    690                 695                 700                 


Lys Thr Phe Tyr Tyr Arg Ala Tyr His Asp Ala Lys Thr Ile Asp Ala 
705                 710                 715                 720 


His Ile Phe Gln Val Leu Phe Glu Pro Val Val 
                725                 730     


<210>  7
<211>  2196
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding TPS2 of Coleus forskohlii - codon optimized for
       expression in S. cerevisiae

<400>  7
atgtccagag ttgcttcctt ggatgctttg aatggtattc aaaaagttgg tccagctacc       60

attggtactc cagaagaaga aaacaagaag atcgaagatt ccatcgaata cgtcaaagaa      120

ttattgaaaa ccatgggtga cggtagaatc tctgtttctc catatgatac tgctatcgtc      180

gccttgatta aggatttgga aggtggtgat ggtccagaat ttccatcttg tttggaatgg      240

attgcccaaa atcaattggc tgatggttct tggggtgatc attttttctg tatctacgat      300

agagttgtta acaccgctgc ttgtgttgtt gctttgaaat cttggaatgt tcacgccgat      360

aagattgaaa aaggtgccgt ttacttgaaa gaaaacgtcc acaaattgaa ggacggtaag      420

atagaacata tgccagctgg ttttgaattc gttgttccag caactttgga aagagctaaa      480

gctttgggta ttaagggttt gccatatgat gatccattca tcagagaaat ctactccgct      540

aagcaaacta gattgactaa gattccaaag ggtatgatct acgaatctcc aacctctttg      600

ttgtactctt tggatggttt agaaggtttg gaatgggata agatcttgaa gttgcaatca      660

gctgacggtt ctttcatcac ttctgtttct tctactgcct tcgttttcat gcataccaac      720

gatttgaagt gccatgcctt tattaagaac gctttgacta actgtaatgg tggtgttcca      780

catacttacc cagttgatat ttttgctaga ttgtgggccg ttgacagatt gcaaagattg      840

ggtatttcta gattcttcga accagaaatc aaatacttga tggaccacat caacaacgtt      900

tggagagaaa agggtgtttt ctcatccaga cattctcaat tcgccgatat tgatgatacc      960

tccatgggta tcagattatt gaagatgcat ggttacaacg ttaacccaaa cgctttggaa     1020

catttcaagc aaaaggatgg taaattcacc tgttacgccg atcaacatat tgaatctcca     1080

tctccaatgt ataacttgta cagagctgcc caattgagat ttccaggtga agaaatttta     1140

caacaagcct tgcaattcgc ctacaacttc ttgcacgaaa atttggcttc taaccacttc     1200

caagaaaagt gggttatctc cgatcatttg atcgatgaag ttagaatcgg tttgaaaatg     1260

ccatggtatg ctactttgcc aagagttgaa gcttcttact acttgcaaca ttacggtggt     1320

tcttccgatg tttggattgg taaaaccttg tatagaatgc cagaaatctc taacgacacc     1380

tacaagattt tggctcaatt ggatttcaac aagtgccaag ctcaacatca attagaatgg     1440

atgtctatga aggaatggta tcaatccaac aacgtaaaag aattcggtat ctccaagaaa     1500

gaattgttgt tggcttactt tttggctgct gctactatgt ttgaacctga aagaactcaa     1560

gaaagaatca tgtgggctaa gacccaagtt gtttctagaa tgattacctc attcttgaac     1620

aaagaaaaca ctatgtcctt cgacttgaag attgctttgt tgactcaacc acaacaccaa     1680

atcaatggtt ccgaaatgaa gaatggtttg gcacaaactt taccagctgc cttcagacaa     1740

ttattgaaag aattcgacaa gtacaccaga caccaattga gaaatacttg gaacaagtgg     1800

ttgatgaagt tgaagcaagg tgatgataac ggtggtgctg atgctgaatt attggctaac     1860

actttgaaca tttgcgccgg tcataacgaa gatattttgt cccattacga atacaccgcc     1920

ttgtcatctt tgaccaacaa gatttgtcaa agattgtccc aaatccaaga taagaagatg     1980

ttggaaatcg aagaaggttc catcaaggac aaagaaatgg aattggaaat tcaaaccttg     2040

gtcaagttgg tattgcaaga aacttctggt ggtatcgaca gaaacatcaa gcaaactttc     2100

ttgtccgttt tcaagacctt ctactacaga gcttaccatg atgctaagac cattgatgcc     2160

catatcttcc aagttttgtt cgaacctgtt gtttaa                               2196


<210>  8
<211>  1704
<212>  DNA
<213>  Artificial sequence

<220>
<223>  cDNA sequence encoding Coleus forskohlii TPS3

<400>  8
atgatcacct ctaaatcatc tgcagctgtt aaatgcagcc tcaccacgcc aacagatttg       60

atggggaaaa taaaagaggt cttcaacagg gaagtcgata cttctccggc agccatgact      120

actcattcta cagatatacc ctctaatctc tgcataatcg acaccctcca gaggctggga      180

atcgaccaat acttccaatc cgaaatcgac gctgttctac atgatacata caggttatgg      240

caactgaaaa agaaagatat attttcggat attactactc atgcaatggc gttcagactt      300

ttgcgagtca aaggatatga agttgcatca gacgaactgg ctccatacgc tgatcaagag      360

cgcattaacc tgcaaaccat tgatgtgccg acagttgttg agctatacag agcagcacag      420

gagagattaa ctgaagaaga tagcactctt gagaaactgt atgtttggac cagcgccttt      480

ctgaagcagc agttgctcac tgatgccatt cctgacaaga aattgcacaa acaagtggaa      540

tactacttga agaactacca tggcatatta gatagaatgg gagtgagacg aaacctcgac      600

ctatatgaca taagccatta taaaagtctc aaagctgctc acaggttcta taatctgagt      660

aatgaagata tcctagcatt tgcgaggcaa gattttaata ttagccaagc ccaacaccag      720

aaagaacttc agcagctgca aaggtggtat gcagattgta ggttggacac gttgaaattt      780

ggaagagatg tagtgcgtat aggaaatttt ctgacttcag caatgattgg tgatcctgaa      840

ttgtctgacc tccgtctagc gtttgccaaa catatagtgc tcgtaacacg tattgatgat      900

tttttcgatc acggtgggcc taaagaagaa tcatacgaga tccttgaatt agtaaaagaa      960

tggaaagaga agccagcagg agaatatgtt tctgaagaag ttgaaatcct atttacagca     1020

gtatacaata cagtaaacga gttggcagaa atggctcata tcgaacaagg acgaagcgtt     1080

aaagaccttc tagttaaact gtgggttgaa atactatcag ttttcagaat agaattggat     1140

acatggacca acgacacagc acttacctta gaagagtact tgtcacaatc ctgggtgtcc     1200

attggctgca gaatctgcat tctcatatca atgcaattcc aaggtgtaaa attatctgat     1260

gaaatgcttc agagtgaaga atgcactgat ttgtgtcggt atgtttcaat ggttgaccgg     1320

ctgctcaacg atgtgcaaac ttttgagaag gaacgcaagg aaaatacagg aaatagtgtg     1380

agccttctgc aagcagctca caaagatgaa agagtcatta atgaagagga agcttgtata     1440

aaggtaaaag aattggctga atataacagg agaaaactga tgcagattgt ctacaaaaca     1500

ggaaccattt tcccaagaaa atgcaaagat ctgtttttga aggcatgcag aattggttgt     1560

tatttgtact caagtggcga cgaatttact tcgcctcaac aaatgatgga agatatgaag     1620

tcactggttt atgaacccct accaatttct cctcctgaag ctaataatgc aagtggagaa     1680

aaaatgagtt gtgtcagcaa ctag                                            1704


<210>  9
<211>  567
<212>  PRT
<213>  Coleus forskohlii

<400>  9

Met Ile Thr Ser Lys Ser Ser Ala Ala Val Lys Cys Ser Leu Thr Thr 
1               5                   10                  15      


Pro Thr Asp Leu Met Gly Lys Ile Lys Glu Val Phe Asn Arg Glu Val 
            20                  25                  30          


Asp Thr Ser Pro Ala Ala Met Thr Thr His Ser Thr Asp Ile Pro Ser 
        35                  40                  45              


Asn Leu Cys Ile Ile Asp Thr Leu Gln Arg Leu Gly Ile Asp Gln Tyr 
    50                  55                  60                  


Phe Gln Ser Glu Ile Asp Ala Val Leu His Asp Thr Tyr Arg Leu Trp 
65                  70                  75                  80  


Gln Leu Lys Lys Lys Asp Ile Phe Ser Asp Ile Thr Thr His Ala Met 
                85                  90                  95      


Ala Phe Arg Leu Leu Arg Val Lys Gly Tyr Glu Val Ala Ser Asp Glu 
            100                 105                 110         


Leu Ala Pro Tyr Ala Asp Gln Glu Arg Ile Asn Leu Gln Thr Ile Asp 
        115                 120                 125             


Val Pro Thr Val Val Glu Leu Tyr Arg Ala Ala Gln Glu Arg Leu Thr 
    130                 135                 140                 


Glu Glu Asp Ser Thr Leu Glu Lys Leu Tyr Val Trp Thr Ser Ala Phe 
145                 150                 155                 160 


Leu Lys Gln Gln Leu Leu Thr Asp Ala Ile Pro Asp Lys Lys Leu His 
                165                 170                 175     


Lys Gln Val Glu Tyr Tyr Leu Lys Asn Tyr His Gly Ile Leu Asp Arg 
            180                 185                 190         


Met Gly Val Arg Arg Asn Leu Asp Leu Tyr Asp Ile Ser His Tyr Lys 
        195                 200                 205             


Ser Leu Lys Ala Ala His Arg Phe Tyr Asn Leu Ser Asn Glu Asp Ile 
    210                 215                 220                 


Leu Ala Phe Ala Arg Gln Asp Phe Asn Ile Ser Gln Ala Gln His Gln 
225                 230                 235                 240 


Lys Glu Leu Gln Gln Leu Gln Arg Trp Tyr Ala Asp Cys Arg Leu Asp 
                245                 250                 255     


Thr Leu Lys Phe Gly Arg Asp Val Val Arg Ile Gly Asn Phe Leu Thr 
            260                 265                 270         


Ser Ala Met Ile Gly Asp Pro Glu Leu Ser Asp Leu Arg Leu Ala Phe 
        275                 280                 285             


Ala Lys His Ile Val Leu Val Thr Arg Ile Asp Asp Phe Phe Asp His 
    290                 295                 300                 


Gly Gly Pro Lys Glu Glu Ser Tyr Glu Ile Leu Glu Leu Val Lys Glu 
305                 310                 315                 320 


Trp Lys Glu Lys Pro Ala Gly Glu Tyr Val Ser Glu Glu Val Glu Ile 
                325                 330                 335     


Leu Phe Thr Ala Val Tyr Asn Thr Val Asn Glu Leu Ala Glu Met Ala 
            340                 345                 350         


His Ile Glu Gln Gly Arg Ser Val Lys Asp Leu Leu Val Lys Leu Trp 
        355                 360                 365             


Val Glu Ile Leu Ser Val Phe Arg Ile Glu Leu Asp Thr Trp Thr Asn 
    370                 375                 380                 


Asp Thr Ala Leu Thr Leu Glu Glu Tyr Leu Ser Gln Ser Trp Val Ser 
385                 390                 395                 400 


Ile Gly Cys Arg Ile Cys Ile Leu Ile Ser Met Gln Phe Gln Gly Val 
                405                 410                 415     


Lys Leu Ser Asp Glu Met Leu Gln Ser Glu Glu Cys Thr Asp Leu Cys 
            420                 425                 430         


Arg Tyr Val Ser Met Val Asp Arg Leu Leu Asn Asp Val Gln Thr Phe 
        435                 440                 445             


Glu Lys Glu Arg Lys Glu Asn Thr Gly Asn Ser Val Ser Leu Leu Gln 
    450                 455                 460                 


Ala Ala His Lys Asp Glu Arg Val Ile Asn Glu Glu Glu Ala Cys Ile 
465                 470                 475                 480 


Lys Val Lys Glu Leu Ala Glu Tyr Asn Arg Arg Lys Leu Met Gln Ile 
                485                 490                 495     


Val Tyr Lys Thr Gly Thr Ile Phe Pro Arg Lys Cys Lys Asp Leu Phe 
            500                 505                 510         


Leu Lys Ala Cys Arg Ile Gly Cys Tyr Leu Tyr Ser Ser Gly Asp Glu 
        515                 520                 525             


Phe Thr Ser Pro Gln Gln Met Met Glu Asp Met Lys Ser Leu Val Tyr 
    530                 535                 540                 


Glu Pro Leu Pro Ile Ser Pro Pro Glu Ala Asn Asn Ala Ser Gly Glu 
545                 550                 555                 560 


Lys Met Ser Cys Val Ser Asn 
                565         


<210>  10
<211>  1704
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding TPS3 of Coleus forskohlii - codon optimized for
       expression in S. cerevisiae

<400>  10
atgatcacct ccaaatcttc cgctgctgtt aagtgttctt tgactactcc aactgatttg       60

atgggtaaga tcaaagaagt tttcaacaga gaagttgata cctctccagc tgctatgact      120

actcattcta ctgatattcc atccaacttg tgcatcatcg ataccttgca aagattgggt      180

atcgaccaat acttccaatc cgaaattgat gctgtcttgc atgatactta cagattgtgg      240

caattgaaga agaaggacat cttctctgat attaccactc atgctatggc cttcagatta      300

ttgagagtta agggttacga agttgcctct gatgaattgg ctccatatgc tgatcaagaa      360

agaatcaact tgcaaaccat tgatgttcca accgtcgtcg aattatacag agctgcacaa      420

gaaagattga ccgaagaaga ttctaccttg gaaaagttgt acgtttggac ttctgctttc      480

ttgaagcaac aattattgac cgatgccatc ccagataaga agttgcataa gcaagtcgaa      540

tattacttga agaactacca cggtatcttg gatagaatgg gtgttagaag aaacttggac      600

ttgtacgata tctcccacta caaatctttg aaggctgctc atagattcta caacttgtct      660

aacgaagata ttttggcctt cgccagacaa gatttcaaca tttctcaagc ccaacaccaa      720

aaagaattgc aacaattgca aagatggtac gccgattgca gattggatac tttgaaattc      780

ggtagagatg tcgtcagaat cggtaacttt ttaacctctg ctatgatcgg tgatccagaa      840

ttgtctgatt tgagattggc ttttgctaag cacatcgttt tggttaccag aatcgatgat      900

ttcttcgatc atggtggtcc aaaagaagaa tcctacgaaa ttttggaatt ggtcaaagaa      960

tggaaagaaa agccagctgg tgaatacgtt tctgaagaag tcgaaatctt attcaccgct     1020

gtttacaaca ccgttaacga attggctgaa atggcccata ttgaacaagg tagatctgtt     1080

aaggatttgt tggttaagtt gtgggtcgaa atattgtccg ttttcagaat cgaattggat     1140

acctggacta acgatactgc tttgactttg gaagaatact tgtcccaatc ctgggtttct     1200

attggttgca gaatctgcat tttgatctcc atgcaattcc aaggtgttaa gttgagtgac     1260

gaaatgttgc aaagtgaaga atgtaccgat ttgtgcagat acgtttccat ggtcgataga     1320

ttattgaacg atgtccaaac cttcgaaaaa gaaagaaaag aaaacaccgg taactccgtt     1380

tctttgttgc aagctgctca caaagacgaa agagttatca acgaagaaga agcctgcatc     1440

aaggtaaaag aattagccga atacaataga agaaagttga tgcaaatcgt ctacaagacc     1500

ggtactattt tcccaagaaa atgcaaggac ttgttcttga aggcttgtag aattggttgc     1560

tacttgtact cttctggtga tgaattcact tccccacaac aaatgatgga agatatgaag     1620

tccttggtct atgaaccatt gccaatttct ccacctgaag ctaacaatgc atctggtgaa     1680

aaaatgtcct gcgtcagtaa ctga                                            1704


<210>  11
<211>  587
<212>  PRT
<213>  Coleus forskohlii

<400>  11

Met Ser Ile Thr Ile Asn Leu Arg Val Ile Ala Phe Pro Gly His Gly 
1               5                   10                  15      


Val Gln Ser Arg Gln Gly Ile Phe Ala Val Met Glu Phe Pro Arg Asn 
            20                  25                  30          


Lys Asn Thr Phe Lys Ser Ser Phe Ala Val Lys Cys Ser Leu Ser Thr 
        35                  40                  45              


Pro Thr Asp Leu Met Gly Lys Ile Lys Glu Lys Leu Ser Glu Lys Val 
    50                  55                  60                  


Asp Asn Ser Val Ala Ala Met Ala Thr Asp Ser Ala Asp Met Pro Thr 
65                  70                  75                  80  


Asn Leu Cys Ile Val Asp Ser Leu Gln Arg Leu Gly Val Glu Lys Tyr 
                85                  90                  95      


Phe Gln Ser Glu Ile Asp Thr Val Leu Asp Asp Ala Tyr Arg Leu Trp 
            100                 105                 110         


Gln Leu Lys Gln Lys Asp Ile Phe Ser Asp Ile Thr Thr His Ala Met 
        115                 120                 125             


Ala Phe Arg Leu Leu Arg Val Lys Gly Tyr Asp Val Ser Ser Glu Glu 
    130                 135                 140                 


Leu Ala Pro Tyr Ala Asp Gln Glu Gly Met Asn Leu Gln Thr Ile Asp 
145                 150                 155                 160 


Leu Ala Ala Val Ile Glu Leu Tyr Arg Ala Ala Gln Glu Arg Val Ala 
                165                 170                 175     


Glu Glu Asp Ser Thr Leu Glu Lys Leu Tyr Val Trp Thr Ser Thr Phe 
            180                 185                 190         


Leu Lys Gln Gln Leu Leu Ala Gly Ala Ile Pro Asp Gln Lys Leu His 
        195                 200                 205             


Lys Gln Val Glu Tyr Tyr Leu Lys Asn Tyr His Gly Ile Leu Asp Arg 
    210                 215                 220                 


Met Gly Val Arg Lys Gly Leu Asp Leu Tyr Asp Ala Gly Tyr Tyr Lys 
225                 230                 235                 240 


Ala Leu Lys Ala Ala Asp Arg Leu Val Asp Leu Cys Asn Glu Asp Leu 
                245                 250                 255     


Leu Ala Phe Ala Arg Gln Asp Phe Asn Ile Asn Gln Ala Gln His Arg 
            260                 265                 270         


Lys Glu Leu Glu Gln Leu Gln Arg Trp Tyr Ala Asp Cys Arg Leu Asp 
        275                 280                 285             


Lys Leu Glu Phe Gly Arg Asp Val Val Arg Val Ser Asn Phe Leu Thr 
    290                 295                 300                 


Ser Ala Ile Leu Gly Asp Pro Glu Leu Ser Glu Val Arg Leu Val Phe 
305                 310                 315                 320 


Ala Lys His Ile Val Leu Val Thr Arg Ile Asp Asp Phe Phe Asp His 
                325                 330                 335     


Gly Gly Pro Arg Glu Glu Ser His Lys Ile Leu Glu Leu Ile Lys Glu 
            340                 345                 350         


Trp Lys Glu Lys Pro Ala Gly Glu Tyr Val Ser Lys Glu Val Glu Ile 
        355                 360                 365             


Leu Tyr Thr Ala Val Tyr Asn Thr Val Asn Glu Leu Ala Glu Arg Ala 
    370                 375                 380                 


Asn Val Glu Gln Gly Arg Asn Val Glu Pro Phe Leu Arg Thr Leu Trp 
385                 390                 395                 400 


Val Gln Ile Leu Ser Ile Phe Lys Ile Glu Leu Asp Thr Trp Ser Asp 
                405                 410                 415     


Asp Thr Ala Leu Thr Leu Asp Asp Tyr Leu Asn Asn Ser Trp Val Ser 
            420                 425                 430         


Ile Gly Cys Arg Ile Cys Ile Leu Met Ser Met Gln Phe Ile Gly Met 
        435                 440                 445             


Lys Leu Pro Glu Glu Met Leu Leu Ser Glu Glu Cys Val Asp Leu Cys 
    450                 455                 460                 


Arg His Val Ser Met Val Asp Arg Leu Leu Asn Asp Val Gln Thr Phe 
465                 470                 475                 480 


Glu Lys Glu Arg Lys Glu Asn Thr Gly Asn Ala Val Ser Leu Leu Leu 
                485                 490                 495     


Ala Ala His Lys Gly Glu Arg Ala Phe Ser Glu Glu Glu Ala Ile Ala 
            500                 505                 510         


Lys Ala Lys Tyr Leu Ala Asp Cys Asn Arg Arg Ser Leu Met Gln Ile 
        515                 520                 525             


Val Tyr Lys Thr Gly Thr Ile Phe Pro Arg Lys Cys Lys Asp Met Phe 
    530                 535                 540                 


Leu Lys Val Cys Arg Ile Gly Cys Tyr Leu Tyr Ala Ser Gly Asp Glu 
545                 550                 555                 560 


Phe Thr Ser Pro Gln Gln Met Met Glu Asp Met Lys Ser Leu Val Tyr 
                565                 570                 575     


Glu Pro Leu Gln Ile His Pro Pro Pro Ala Asn 
            580                 585         


<210>  12
<211>  1764
<212>  DNA
<213>  Coleus forskohlii

<400>  12
atgtcaatca ccatcaacct tcgagttatc gctttccccg gccatggagt tcagagcagg       60

caaggaatat ttgcagtcat ggaatttcca aggaacaaga acacctttaa atcatccttt      120

gctgttaaat gcagcctctc tactccaaca gatttgatgg gaaagataaa agaaaagttg      180

agcgagaagg ttgataattc tgtggcagcc atggctactg attctgccga tatgcccact      240

aatctctgca tcgtcgactc cctccagagg ctgggagtcg aaaaatattt ccaatccgaa      300

atcgacactg ttctcgatga tgcataccgg ttatggcagc tgaagcagaa agatatattt      360

tcagacatta ctactcatgc aatggcgttt agacttctgc gagtcaaagg atacgatgtt      420

tcatcagagg agctggctcc atacgctgat caagagggca tgaacttgca aacgattgat      480

ctggcggcgg tcatcgagct gtacagagca gcacaggaga gagtggctga ggaagacagc      540

actcttgaga aactgtatgt ctggaccagc acctttctga agcagcagtt gctggctggc      600

gccattcctg accagaaatt gcacaaacag gtggagtact acttgaagaa ctaccacggc      660

atattagata gaatgggagt tagaaaagga ctcgacctgt atgatgctgg ctattacaag      720

gccctcaaag ctgcagatag gttggttgat ctatgcaatg aagaccttct agcatttgca      780

aggcaagatt ttaatattaa ccaagcccaa caccgcaaag aacttgagca actgcaaagg      840

tggtatgcag attgtaggtt ggacaaactc gagtttggaa gagatgtggt gcgtgtatcg      900

aattttctga cttcagccat ccttggtgat ccagagcttt ctgaagtccg tctagtgttt      960

gccaaacata ttgtgctagt gactaggata gatgattttt tcgatcatgg cgggcctaga     1020

gaagaatcac acaagatcct tgaactaata aaagaatgga aagagaagcc agctggagaa     1080

tatgtttcca aagaagttga gatcctatat accgcggtgt acaatacggt aaacgagttg     1140

gcagagaggg caaatgttga acaagggcga aatgttgaac catttctacg tacactgtgg     1200

gttcaaatac tgtcgatttt caagatagag ttggatacat ggagcgatga cacagcacta     1260

accttggatg attacttgaa caactcatgg gtgtcgattg gttgtagaat ctgcattctc     1320

atgtccatgc aattcattgg tatgaagtta ccagaagaaa tgcttctcag tgaagagtgc     1380

gttgatttgt gtaggcatgt ttccatggtc gaccgtctgc tcaatgatgt ccaaactttt     1440

gagaaggaac ggaaagaaaa tacaggaaac gctgtgagcc ttctgctagc agctcacaag     1500

ggtgaaagag ccttcagtga agaggaagcc atagcaaaag cgaaatattt ggctgactgt     1560

aacaggagaa gtctgatgca gattgtgtat aaaacaggaa ccattttccc aagaaaatgc     1620

aaagatatgt tcttgaaggt gtgcaggatt ggttgctatt tgtatgcgag tggcgacgaa     1680

tttacttccc ctcaacaaat gatggaagat atgaagtcat tagtttatga gcccctccaa     1740

attcaccctc cacctgctaa ctaa                                            1764


