                         SEQUENCE LISTING

<110>  ApiRays AB
 
<120>  ANALYTICAL AND DIAGNOSTIC METHODS UTILIZING SHIGELLA FLEXNERI 
       APYRASE

<130>  NP0155WO

<150>  SE 1451332-9
<151>  2014-11-07

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1365
<212>  DNA
<213>  Solanum tuberosum

<400>  1
atgttgaacc aaaatagtca ttttgttttc acaattttgg caatattttt ggttttgccc       60

ctaagtttat tatccaaaaa tgtggatgct caaattccat taagaagaca tttattaagt      120

catgaatcag aacattatgc agtaatattt gatgctggaa gtactggaag tagagttcat      180

gtttttcgat ttgatgaaaa attaggactt cttcctattg gcaacaatat tgagtatttt      240

atggcgacag agccaggttt aagttcatat gcagaagatc caaaggctgc agccaattca      300

cttgagccac ttttagatgg agctgaagga gttgttcctc aagaattgca atctgaaaca      360

cctttagaac ttggggcaac agcaggtctt aggatgttaa aaggggatgc agctgaaaaa      420

attctacaag cagtgagaaa tttagtgaag aatcaaagca ctttccatag caaagatcaa      480

tgggtcacta ttcttgatgg aactcaagaa ggctcttata tgtgggctgc aataaattat      540

ttattgggaa atttgggcaa agattataaa agtacaacag caacaattga tcttggtggt      600

ggttcagtcc aaatggcata tgctatatca aatgaacaat ttgcaaaagc tcctaataat      660

gaggatggag aaccttatgt acaacaaaaa catcttatgt caaaagatta taatctctat      720

gtacatagtt atttaaacta tgggcaatta gcaggtcgag ctgagatttt caaggcttca      780

agaaatgaaa gtaatccttg tgctctggaa ggatatgatg ggtattactc atatggagga      840

gtggactaca aagtaaaagc accaaagaaa ggtagtagtt ggaagagatg caggaggtta      900

actagacatg cacttaaaat aaatgcaaaa tgcaagattg aagaatgcac cttcaatgga      960

gtgtggaatg gtggtggtgg tgatggacaa aaaaatattc atgcttcatc atttttttat     1020

gatattggtg ctcaggttgg cattgttgac accaaatttc catctgctct agcaaagcca     1080

attcaatact taaatgcagc taaagttgct tgccaaacaa atgtggcaga tataaaatcc     1140

atattcccaa aaactcaaga tagaaatatc ccatacttat gtatggactt gatatatgag     1200

tacactttgc ttgttgatgg atttggacta aatccacaca aagaaataac agtgatacat     1260

gatgtgcaat acaaaaacta tctagttgga gcagcatggc cattgggatg tgccattgac     1320

ttggtttctt caactacaaa caaaattaga gttgcatcat cttaa                     1365


<210>  2
<211>  1134
<212>  DNA
<213>  Shigella flexneri

<400>  2
tcacaaatca tcataatcaa gagacaaaac gatacgaaaa aataagataa aaaacatcgt       60

tcttttacca cattattttc cgtgaatatg aaaaataatg ttattacttt aatataagac      120

tattttttgt ttttccatca ctctgttcaa atttttccgc atgacttgtg ttttttgtaa      180

tacagctcgt tttttacagc tgaccaaaat catcaattaa ttatgctaag gaaataaatt      240

atgaaaacca aaaactttct tcttttttgt attgctacaa atatgatttt tatcccctca      300

gcaaatgctc tgaaggcaga aggttttctc actcaacaaa cttcaccaga cagtttgtca      360

atacttccgc cgcctccggc agagaattca gtagtatttc aggctgacaa agctcattat      420

gaattcggcc gctcgctccg ggatgctaat cgtgtacgtc tcgctagcga agatgcatac      480

tacgagaatt ttggtcttgc attttcagat gcttatggca tggatatttc aagggaaaat      540

accccaatct tatatcagtt gttaacacaa gtactacagg atagccatga ttacgccgtg      600

cgtaacgcca aagaatatta taaaagagtt cgtccattcg ttatttataa agacgcaacc      660

tgtacacctg ataaagatga gaaaatggct atcactggct cttatccctc tggtcatgca      720

tcctttggtt gggcagtagc actgatactt gcggagatta atcctcaacg taaagcggaa      780

atacttcgac gtggatatga gtttggagaa agtcgggtca tctgcggtgc gcattggcaa      840

agcgatgtag aggctgggcg tttaatggga gcatcggttg ttgcagtact tcataataca      900

cctgaattta ccaaaagcct tagcgaagcc aaaaaagagt ttgaagaatt aaatactcct      960

accaatgaac tgaccccata aagctggaca gcctgtatca ggctatggag ggcccataga     1020

caaatctacc ctatatgagc ataggaggag tctatgggca caccacgttt tacccctgaa     1080

tttaagggat tactggaaag gctgggacat atcctccggc agaagcagaa aaag           1134


<210>  3
<211>  780
<212>  DNA
<213>  Shigella flexneri

<400>  3
gatatacata tgaaaaccaa aaactttctt cttttttgta ttgctacaaa tatgattttt       60

atcccctcag caaatgctct gaaggcagaa ggttttctca ctcaacaaac ttcaccagac      120

agtttgtcaa tacttccgcc gcctccggca gagaattcag tagtatttca ggctgacaaa      180

gctcattatg aattcggccg ctcgctccgg gatgctaatc gtgtacgtct cgctagcgaa      240

gatgcatact acgagaattt tggtcttgca ttttcagatg cttatggcat ggatatttca      300

agggaaaata ccccaatctt atatcagttg ttaacacaag tactacagga tagccatgat      360

tacgccgtgc gtaacgccaa agaatattat aaaagagttc gtccattcgt tatttataaa      420

gacgcaacct gtacacctga taaagatgag aaaatggcta tcactggctc ttatccctct      480

ggtcatgcat cctttggttg ggcagtagca ctgatacttg cggagattaa tcctcaacgt      540

aaagcggaaa tacttcgacg tggatatgag tttggagaaa gtcgggtcat ctgcggtgcg      600

cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag catcggttgt tgcagtactt      660

cataatacac ctgaatttac caaaagcctt agcgaagcca aaaaagagtt tgaagaatta      720

aatactccta ccaatgaact gaccccacat catcaccatc accactaacg tacgatgttc      780


<210>  4
<211>  259
<212>  PRT
<213>  Shigella flexneri

<400>  4

Asp Ile His Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr 
1               5                   10                  15      


Asn Met Ile Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe 
            20                  25                  30          


Leu Thr Gln Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro 
        35                  40                  45              


Pro Ala Glu Asn Ser Val Val Phe Gln Ala Asp Lys Ala His Tyr Glu 
    50                  55                  60                  


Phe Gly Arg Ser Leu Arg Asp Ala Asn Arg Val Arg Leu Ala Ser Glu 
65                  70                  75                  80  


Asp Ala Tyr Tyr Glu Asn Phe Gly Leu Ala Phe Ser Asp Ala Tyr Gly 
                85                  90                  95      


Met Asp Ile Ser Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Leu Thr 
            100                 105                 110         


Gln Val Leu Gln Asp Ser His Asp Tyr Ala Val Arg Asn Ala Lys Glu 
        115                 120                 125             


Tyr Tyr Lys Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys 
    130                 135                 140                 


Thr Pro Asp Lys Asp Glu Lys Met Ala Ile Thr Gly Ser Tyr Pro Ser 
145                 150                 155                 160 


Gly His Ala Ser Phe Gly Trp Ala Val Ala Leu Ile Leu Ala Glu Ile 
                165                 170                 175     


Asn Pro Gln Arg Lys Ala Glu Ile Leu Arg Arg Gly Tyr Glu Phe Gly 
            180                 185                 190         


Glu Ser Arg Val Ile Cys Gly Ala His Trp Gln Ser Asp Val Glu Ala 
        195                 200                 205             


Gly Arg Leu Met Gly Ala Ser Val Val Ala Val Leu His Asn Thr Pro 
    210                 215                 220                 


Glu Phe Thr Lys Ser Leu Ser Glu Ala Lys Lys Glu Phe Glu Glu Leu 
225                 230                 235                 240 


Asn Thr Pro Thr Asn Glu Leu Thr Pro His His His His His His Arg 
                245                 250                 255     


Thr Met Phe 
            


<210>  5
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  5
cgcggatccc tgaaggcaga aggttttc                                          28


<210>  6
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  6
cccaagcttt tatggggtca gttcatt                                           27


<210>  7
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  7
gaacatcgta cgttagtggt gatggtgatg atgtggggtc agttcattgg tag              53


<210>  8
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  8
gatatacata tgaaaaccaa aaactttc                                          28


<210>  9
<211>  454
<212>  PRT
<213>  Solanum tuberosum

<400>  9

Met Leu Asn Gln Asn Ser His Phe Ile Phe Ile Ile Leu Ala Ile Phe 
1               5                   10                  15      


Leu Val Leu Pro Leu Ser Leu Leu Ser Lys Asn Val Asn Ala Gln Ile 
            20                  25                  30          


Pro Leu Arg Arg His Leu Leu Ser His Glu Ser Glu His Tyr Ala Val 
        35                  40                  45              


Ile Phe Asp Ala Gly Ser Thr Gly Ser Arg Val His Val Phe Arg Phe 
    50                  55                  60                  


Asp Glu Lys Leu Gly Leu Leu Pro Ile Gly Asn Asn Ile Glu Tyr Phe 
65                  70                  75                  80  


Met Ala Thr Glu Pro Gly Leu Ser Ser Tyr Ala Glu Asp Pro Lys Ala 
                85                  90                  95      


Ala Ala Asn Ser Leu Glu Pro Leu Leu Asp Gly Ala Glu Gly Val Val 
            100                 105                 110         


Pro Gln Glu Leu Gln Ser Glu Thr Pro Leu Glu Leu Gly Ala Thr Ala 
        115                 120                 125             


Gly Leu Arg Met Leu Lys Gly Asp Ala Ala Glu Lys Ile Leu Gln Ala 
    130                 135                 140                 


Val Arg Asn Leu Val Lys Asn Gln Ser Thr Phe His Ser Lys Asp Gln 
145                 150                 155                 160 


Trp Val Thr Ile Leu Asp Gly Thr Gln Glu Gly Ser Tyr Met Trp Ala 
                165                 170                 175     


Ala Ile Asn Tyr Leu Leu Gly Asn Leu Gly Lys Asp Tyr Lys Ser Thr 
            180                 185                 190         


Thr Ala Thr Ile Asp Leu Gly Gly Gly Ser Val Gln Met Ala Tyr Ala 
        195                 200                 205             


Ile Ser Asn Glu Gln Phe Ala Lys Ala Pro Gln Asn Glu Asp Gly Glu 
    210                 215                 220                 


Pro Tyr Val Gln Gln Lys His Leu Met Ser Lys Asp Tyr Asn Leu Tyr 
225                 230                 235                 240 


Val His Ser Tyr Leu Asn Tyr Gly Gln Leu Ala Gly Arg Ala Glu Ile 
                245                 250                 255     


Phe Lys Ala Ser Arg Asn Glu Ser Asn Pro Cys Ala Leu Glu Gly Cys 
            260                 265                 270         


Asp Gly Tyr Tyr Ser Tyr Gly Gly Val Asp Tyr Lys Val Lys Ala Pro 
        275                 280                 285             


Lys Lys Gly Ser Ser Trp Lys Arg Cys Arg Arg Leu Thr Arg His Ala 
    290                 295                 300                 


Leu Lys Ile Asn Ala Lys Cys Asn Ile Glu Glu Cys Thr Phe Asn Gly 
305                 310                 315                 320 


Val Trp Asn Gly Gly Gly Gly Asp Gly Gln Lys Asn Ile His Ala Ser 
                325                 330                 335     


Ser Phe Phe Tyr Asp Ile Gly Ala Gln Val Gly Ile Val Asp Thr Lys 
            340                 345                 350         


Phe Pro Ser Ala Leu Ala Lys Pro Ile Gln Tyr Leu Asn Ala Ala Lys 
        355                 360                 365             


Val Ala Cys Gln Thr Asn Val Ala Asp Ile Lys Ser Ile Phe Pro Lys 
    370                 375                 380                 


Thr Gln Asp Arg Asn Ile Pro Tyr Leu Cys Met Asp Leu Ile Tyr Glu 
385                 390                 395                 400 


Tyr Thr Leu Leu Val Asp Gly Phe Gly Leu Asn Pro His Lys Glu Ile 
                405                 410                 415     


Thr Val Ile His Asp Val Gln Tyr Lys Asn Tyr Leu Val Gly Ala Ala 
            420                 425                 430         


Trp Pro Leu Gly Cys Ala Ile Asp Leu Val Ser Ser Thr Thr Asn Lys 
        435                 440                 445             


Ile Arg Val Ala Ser Ser 
    450                 


<210>  10
<211>  246
<212>  PRT
<213>  Shigella flexneri

<400>  10

Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr Asn Met Ile 
1               5                   10                  15      


Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe Leu Thr Gln 
            20                  25                  30          


Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro Pro Ala Glu 
        35                  40                  45              


Asn Ser Val Val Phe Gln Ala Asp Lys Ala His Tyr Glu Phe Gly Arg 
    50                  55                  60                  


Ser Leu Arg Asp Ala Asn Arg Val Arg Leu Ala Ser Glu Asp Ala Tyr 
65                  70                  75                  80  


Tyr Glu Asn Phe Gly Leu Ala Phe Ser Asp Ala Tyr Gly Met Asp Ile 
                85                  90                  95      


Ser Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Leu Thr Gln Val Leu 
            100                 105                 110         


Gln Asp Ser His Asp Tyr Ala Val Arg Asn Ala Lys Glu Tyr Tyr Lys 
        115                 120                 125             


Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys Thr Pro Asp 
    130                 135                 140                 


Lys Asp Glu Lys Met Ala Ile Thr Gly Ser Tyr Pro Ser Gly His Ala 
145                 150                 155                 160 


Ser Phe Gly Trp Ala Val Ala Leu Ile Leu Ala Glu Ile Asn Pro Gln 
                165                 170                 175     


Arg Lys Ala Glu Ile Leu Arg Arg Gly Tyr Glu Phe Gly Glu Ser Arg 
            180                 185                 190         


Val Ile Cys Gly Ala His Trp Gln Ser Asp Val Glu Ala Gly Arg Leu 
        195                 200                 205             


Met Gly Ala Ser Val Val Ala Val Leu His Asn Thr Pro Glu Phe Thr 
    210                 215                 220                 


Lys Ser Leu Ser Glu Ala Lys Lys Glu Phe Glu Glu Leu Asn Thr Pro 
225                 230                 235                 240 


Thr Asn Glu Leu Thr Pro 
                245     


