                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH and Hoffmann-La Roche AG
       
 
<120>  Recombinant Trypanosoma cruzi JL7 antigen variants and their use 
       for detecting Chagas disease

<130>  P32734 WO-IR

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  226
<212>  PRT
<213>  Trypanosoma cruzi

<400>  1

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Ala Arg Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80  


Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
                85                  90                  95      


Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
            100                 105                 110         


Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
        115                 120                 125             


Asp Asp Asp Ser Asp Phe Val Ser Met Glu Gln Glu Arg Arg Gln Leu 
    130                 135                 140                 


Leu Glu Lys Asp Pro Arg Arg Asn Val Gln Lys Ile Ala Asp Leu Glu 
145                 150                 155                 160 


Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
                165                 170                 175     


Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Ser Leu Arg 
            180                 185                 190         


Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ser Met Glu Gln Glu 
        195                 200                 205             


Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Lys Asn Val Gln Ile Val 
    210                 215                 220                 


Ala Asp 
225     


<210>  2
<211>  68
<212>  PRT
<213>  Trypanosoma cruzi

<400>  2

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ala 
65              


<210>  3
<211>  68
<212>  PRT
<213>  Trypanosoma cruzi

<400>  3

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ser 
65              


<210>  4
<211>  136
<212>  PRT
<213>  Trypanosoma cruzi

<400>  4

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Ala Arg Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80  


Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
                85                  90                  95      


Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
            100                 105                 110         


Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
        115                 120                 125             


Asp Asp Asp Ser Asp Phe Val Ser 
    130                 135     


<210>  5
<211>  90
<212>  PRT
<213>  Trypanosoma cruzi

<400>  5

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Val Gln Lys Ile Ala Asp Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Ser Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ser Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80  


Pro Arg Lys Asn Val Gln Ile Val Ala Asp 
                85                  90  


<210>  6
<211>  68
<212>  PRT
<213>  Trypanosoma cruzi

<400>  6

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Val Gln Lys Ile Ala Asp Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Ser Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ser 
65              


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hexa-histidine tag

<400>  7

Gly Gly Gly Ser Gly Gly Gly Leu Glu His His His His His His 
1               5                   10                  15  


