                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH
 
<120>  Antigen composition for detecting Chagas disease

<130>  P32403 WO-IR

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from UniProt Q4D1Q2 Trypanosoma cruzi 1F8 with variation 
       X= Cys, Ala or Ser


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be Cys, Ala or Ser

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Xaa can be Cys, Ala or Ser

<220>
<221>  misc_feature
<222>  (74)..(74)
<223>  Xaa can be Cys, Ala or Ser

<220>
<221>  misc_feature
<222>  (124)..(124)
<223>  Xaa can be Cys, Ala or Ser

<400>  1

Met Gly Ala Xaa Gly Ser Lys Gly Ser Thr Ser Asp Lys Gly Leu Ala 
1               5                   10                  15      


Ser Asp Lys Asp Gly Lys Asn Ala Lys Asp Arg Lys Glu Ala Trp Glu 
            20                  25                  30          


Arg Ile Arg Gln Ala Ile Pro Arg Glu Lys Thr Ala Glu Ala Lys Gln 
        35                  40                  45              


Arg Arg Ile Glu Leu Phe Lys Lys Phe Asp Lys Asn Glu Thr Gly Lys 
    50                  55                  60                  


Leu Xaa Tyr Asp Glu Val His Ser Gly Xaa Leu Glu Val Leu Lys Leu 
65                  70                  75                  80  


Asp Glu Phe Thr Pro Arg Val Arg Asp Ile Thr Lys Arg Ala Phe Asp 
                85                  90                  95      


Lys Ala Arg Ala Leu Gly Ser Lys Leu Glu Asn Lys Gly Ser Glu Asp 
            100                 105                 110         


Phe Val Glu Phe Leu Glu Phe Arg Leu Met Leu Xaa Tyr Ile Tyr Asp 
        115                 120                 125             


Phe Phe Glu Leu Thr Val Met Phe Asp Glu Ile Asp Ala Ser Gly Asn 
    130                 135                 140                 


Met Leu Val Asp Glu Glu Glu Leu Lys Arg Ala Val Pro Lys Leu Glu 
145                 150                 155                 160 


Ala Trp Gly Ala Lys Val Glu Asp Pro Ala Ala Leu Phe Lys Glu Leu 
                165                 170                 175     


Asp Lys Asn Gly Thr Gly Ser Val Thr Phe Asp Glu Phe Ala Ala Trp 
            180                 185                 190         


Ala Ser Ala Val Lys Leu Asp Ala Asp Gly Asp Pro Asp Asn Val Pro 
        195                 200                 205             


Glu Ser Ala 
    210     


<210>  2
<211>  226
<212>  PRT
<213>  Trypanosoma cruzi

<400>  2

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15      


Ala Arg Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30          


Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45              


Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60                  


Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80  


Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
                85                  90                  95      


Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
            100                 105                 110         


Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
        115                 120                 125             


Asp Asp Asp Ser Asp Phe Val Ser Met Glu Gln Glu Arg Arg Gln Leu 
    130                 135                 140                 


Leu Glu Lys Asp Pro Arg Arg Asn Val Gln Lys Ile Ala Asp Leu Glu 
145                 150                 155                 160 


Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
                165                 170                 175     


Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Ser Leu Arg 
            180                 185                 190         


Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ser Met Glu Gln Glu 
        195                 200                 205             


Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Lys Asn Val Gln Ile Val 
    210                 215                 220                 


Ala Asp 
225     


<210>  3
<211>  130
<212>  PRT
<213>  Trypanosoma cruzi

<400>  3

Gly Pro Gly Pro Thr Pro Glu Pro Thr Thr Thr Thr Thr Thr Ser Ala 
1               5                   10                  15      


Pro Gly Pro Ser Pro Ser Tyr Phe Val Gln Met Ser Cys Thr Asp Ala 
            20                  25                  30          


Ala Cys Ile Val Gly Cys Glu Asn Val Thr Leu Pro Thr Gly Gln Cys 
        35                  40                  45              


Leu Leu Thr Thr Ser Gly Val Ser Ala Ile Val Thr Cys Gly Ala Glu 
    50                  55                  60                  


Thr Leu Thr Glu Glu Val Phe Leu Thr Ser Thr His Cys Ser Gly Pro 
65                  70                  75                  80  


Ser Val Arg Ser Ser Val Pro Leu Asn Lys Cys Asn Arg Leu Leu Arg 
                85                  90                  95      


Gly Ser Val Glu Phe Phe Cys Gly Ser Ser Ser Ser Gly Arg Leu Ala 
            100                 105                 110         


Asp Val Asp Arg Gln Arg Arg His Gln Pro Tyr His Ser Arg His Arg 
        115                 120                 125             


Arg Leu 
    130 


<210>  4
<211>  92
<212>  PRT
<213>  Trypanosoma cruzi

<400>  4

Met Ala Thr Thr Leu Glu Glu Phe Ser Ala Lys Leu Asp Arg Leu Asp 
1               5                   10                  15      


Ala Glu Phe Ala Lys Lys Met Glu Glu Gln Asn Lys Lys Phe Phe Ala 
            20                  25                  30          


Asp Lys Pro Asp Glu Ser Thr Leu Ser Pro Glu Met Lys Glu His Tyr 
        35                  40                  45              


Glu Lys Phe Glu Lys Met Ile Gln Glu His Thr Asp Lys Phe Asn Lys 
    50                  55                  60                  


Lys Met His Glu His Ser Glu His Phe Lys Ala Lys Phe Ala Glu Leu 
65                  70                  75                  80  


Leu Glu Gln Gln Lys Asn Ala Gln Phe Pro Gly Lys 
                85                  90          


<210>  5
<211>  324
<212>  PRT
<213>  Trypanosoma cruzi

<400>  5

Phe Gln Glu Thr Ser Ala Ile Lys Asp Ala Lys Arg Arg Leu Lys Gln 
1               5                   10                  15      


Arg Cys Glu Asp Asp Leu Lys Asn Leu His Asp Ala Ile Gln Lys Ala 
            20                  25                  30          


Asp Met Glu Asp Ala Glu Ala Met Lys Arg Phe Ala Thr Gln Lys Glu 
        35                  40                  45              


Lys Ser Glu Lys Phe Ile Gln Glu Asn Leu Asp Arg Gln Asp Glu Ala 
    50                  55                  60                  


Trp Arg Arg Ile Gln Glu Leu Glu Arg Val Leu Gln Arg Leu Gly Thr 
65                  70                  75                  80  


Glu Arg Phe Glu Glu Val Lys Arg Arg Ile Glu Glu Asn Asp Arg Glu 
                85                  90                  95      


Glu Lys Arg Lys Val Glu Tyr Gln Gln Phe Leu Asp Val Cys Gly Gln 
            100                 105                 110         


His Lys Lys Leu Leu Glu Leu Ser Val Tyr Asn Cys Asp Leu Ala Met 
        115                 120                 125             


Arg Cys Ile Gly Met Met Glu Glu Leu Val Ala Glu Gly Cys Ser Ala 
    130                 135                 140                 


Ile Lys Ser Arg His Asp Lys Thr Asn Glu Glu Leu Gly Asp Leu Arg 
145                 150                 155                 160 


Leu Gln Val His Gln Glu Tyr Leu Glu Ala Phe Arg Arg Leu Tyr Lys 
                165                 170                 175     


Thr Leu Gly Gln Leu Val Tyr Lys Lys Glu Lys Arg Leu Glu Glu Ile 
            180                 185                 190         


Asp Arg Asn Ile Arg Thr Thr His Ile Gln Leu Glu Phe Ala Ile Glu 
        195                 200                 205             


Thr Phe Asp Pro Asn Ala Lys Lys His Ser Asp Ala Lys Lys Glu Leu 
    210                 215                 220                 


Tyr Lys Leu Arg Ala Gln Val Glu Glu Glu Leu Glu Met Leu Lys Asp 
225                 230                 235                 240 


Lys Met Ala Gln Ala Leu Glu Met Phe Gly Pro Thr Glu Asp Ala Leu 
                245                 250                 255     


Asn Gln Ala Gly Ile Glu Phe Val His Pro Ala Glu Glu Val Glu Asp 
            260                 265                 270         


Gly Asn Leu Thr Arg Arg Ser Lys Met Val Glu Tyr Arg Ala His Leu 
        275                 280                 285             


Ala Lys Gln Glu Glu Val Lys Ile Ala Ala Glu Arg Glu Glu Leu Lys 
    290                 295                 300                 


Arg Ser Lys Thr Leu Gln Ser Gln Gln Tyr Arg Gly Lys Thr Val Gln 
305                 310                 315                 320 


Gln Ile Thr Gln 
                


<210>  6
<211>  196
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Cys Cys Gly Gly His Gly His Asp His Gly 
                165                 170                 175     


His Glu His Gly Gly Glu Gly Cys Cys Gly Gly Lys Gly Asn Gly Gly 
            180                 185                 190         


Cys Gly Cys His 
        195     


<210>  7
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine-rich linker sequence

<400>  7

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly 
            20              


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hexa-histidine tag

<400>  8

Gly Gly Gly Ser Gly Gly Gly Leu Glu His His His His His His 
1               5                   10                  15  


