                         SEQUENCE LISTING

<110>  Crucell Holland B.V.
       Bunnik, Evelien Margaretha
       Custers, Jerome Hubertina Henricus Victor
       Scheper, Gerrit Christiaan
       Oosterhuis, Koen
       Uil, Taco Gilles
       Khan, Selina
 
<120>  Therapeutic HPV16 vaccines

<130>  0248 WO 00 ORD

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  328
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPV16-E6E7SH designer polypeptide

<400>  1

Met His Gln Lys Arg Thr Ala Met Phe Gln Asp Pro Gln Glu Arg Pro 
1               5                   10                  15      


Arg Lys Leu Pro Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile His Asp 
            20                  25                  30          


Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln Leu Glu Asp Glu Ile 
        35                  40                  45              


Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile 
    50                  55                  60                  


Val Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln 
65                  70                  75                  80  


Ser Thr His Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr 
                85                  90                  95      


Leu Gly Ile Val Cys Pro Ile Cys Ser Gln Lys Pro Gly Thr Thr Leu 
            100                 105                 110         


Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile 
        115                 120                 125             


Asn Cys Gln Lys Pro Leu Cys Pro Glu Glu Lys Gln Arg His Leu Asp 
    130                 135                 140                 


Lys Lys Gln Arg Phe His Asn Ile Arg Gly Arg Trp Thr Gly Arg Cys 
145                 150                 155                 160 


Met Ser Cys Cys Arg Ser Ser Arg Thr Arg Arg Glu Thr Gln Met His 
                165                 170                 175     


Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro Glu 
            180                 185                 190         


Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser Glu Glu 
        195                 200                 205             


Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala 
    210                 215                 220                 


His Tyr Asn Ile Val Thr Phe Cys Cys Gln Leu Cys Thr Glu Leu Gln 
225                 230                 235                 240 


Thr Thr Ile His Asp Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln 
                245                 250                 255     


Leu Leu Arg Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp Leu Cys Ile 
            260                 265                 270         


Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys 
        275                 280                 285             


Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr 
    290                 295                 300                 


Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu 
305                 310                 315                 320 


Ile Arg Cys Ile Asn Cys Gln Lys 
                325             


<210>  2
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding HPV16-E6E7SH designer polypeptide

<400>  2
atgcaccaga aacggaccgc catgttccag gacccccagg aacggcccag aaagctgccc       60

cagctgtgca ccgagctgca gaccaccatc cacgacatca tcctggaatg cgtgtactgc      120

aagcagcagc tggaagatga gatcgacggc cctgctggcc aggccgaacc cgacagagcc      180

cactacaata tcgtgacctt ctgctgcaag tgcgacagca ccctgcggct gtgcgtgcag      240

agcacccacg tggacatccg gaccctggaa gatctgctga tgggcaccct gggcatcgtg      300

tgccccatct gcagccagaa gcccggcacc accctggaac agcagtacaa caagcccctg      360

tgcgacctgc tgatccggtg catcaactgc cagaaacccc tgtgccccga ggaaaagcag      420

cggcacctgg acaagaagca gcggttccac aacatccggg gcagatggac aggcagatgc      480

atgagctgct gcagaagcag ccggaccaga cgggaaaccc agatgcacgg cgacaccccc      540

accctgcacg agtacatgct ggacctgcag cccgagacaa ccgacctgta ctgctacgag      600

cagctgaacg acagcagcga ggaagaggac gagattgacg gacccgctgg acaggccgag      660

cctgaccggg ctcactataa catcgtgaca ttttgctgtc agctctgtac tgaactccag      720

acaacaattc acgatattat tctcgaatgt gtgtattgta aacagcagct cctgcggaga      780

gaggtgtacg acttcgcctt ccgggacctc tgcatcgtgt atcgggacgg caacccctac      840

gccgtgtgcg acaagtgcct gaagttctac agcaagatca gcgagtaccg gcactactgc      900

tacagcctgt acggaacaac actcgaacag cagtataaca aaccactctg tgatctgctg      960

attcgctgta tcaattgtca gaagtgataa                                       990


<210>  3
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPV16 E2E6E7SH designer polypeptide

<400>  3

Met Glu Thr Leu Cys Gln Arg Leu Asn Val Cys Gln Asp Lys Ile Leu 
1               5                   10                  15      


Thr His Tyr Glu Asn Asp Ser Thr Asp Leu Arg Asp His Ile Asp Tyr 
            20                  25                  30          


Trp Lys His Met Arg Leu Glu Cys Ala Ile Tyr Tyr Lys Ala Arg Glu 
        35                  40                  45              


Met Gly Phe Lys His Ile Asn His Gln Val Val Pro Thr Leu Ala Val 
    50                  55                  60                  


Ser Lys Asn Lys Ala Leu Gln Ala Ile Glu Leu Gln Leu Thr Leu Glu 
65                  70                  75                  80  


Thr Ile Tyr Asn Ser Gln Tyr Ser Asn Glu Lys Trp Thr Leu Gln Asp 
                85                  90                  95      


Val Ser Leu Glu Val Tyr Leu Thr Ala Pro Thr Gly Cys Ile Lys Lys 
            100                 105                 110         


His Gly Tyr Thr Val Glu Val Gln Phe Asp Gly Asp Ile Cys Asn Thr 
        115                 120                 125             


Met His Tyr Thr Asn Trp Thr His Ile Tyr Ile Cys Glu Glu Ala Ser 
    130                 135                 140                 


Val Thr Val Val Glu Gly Gln Val Asp Tyr Tyr Gly Leu Tyr Tyr Val 
145                 150                 155                 160 


His Glu Gly Ile Arg Thr Tyr Phe Val Gln Phe Lys Asp Asp Ala Glu 
                165                 170                 175     


Lys Tyr Ser Lys Asn Lys Val Trp Glu Val His Ala Gly Gly Gln Val 
            180                 185                 190         


Ile Leu Cys Pro Thr Ser Val Phe Ser Ser Asn Glu Val Ser Ser Pro 
        195                 200                 205             


Glu Ile Ile Arg Gln His Leu Ala Asn His Pro Ala Ala Thr His Thr 
    210                 215                 220                 


Lys Ala Val Ala Leu Gly Thr Glu Glu Thr Gln Thr Thr Ile Gln Arg 
225                 230                 235                 240 


Pro Arg Ser Glu Pro Asp Thr Gly Asn Pro Cys His Thr Thr Lys Leu 
                245                 250                 255     


Leu His Arg Asp Ser Val Asp Ser Ala Pro Ile Leu Thr Ala Phe Asn 
            260                 265                 270         


Ser Ser His Lys Gly Arg Ile Asn Cys Asn Ser Asn Thr Thr Pro Ile 
        275                 280                 285             


Val His Leu Lys Val Asp Ala Asn Thr Leu Met Arg Leu Arg Tyr Arg 
    290                 295                 300                 


Phe Lys Lys His Cys Thr Leu Tyr Thr Ala Val Ser Ser Thr Trp His 
305                 310                 315                 320 


Trp Thr Gly His Asn Val Lys His Lys Ser Ala Ile Val Thr Leu Thr 
                325                 330                 335     


Tyr Asp Ser Glu Trp Gln Arg Asp Gln Phe Leu Ser Gln Val Lys Ile 
            340                 345                 350         


Pro Lys Thr Ile Thr Val Ser Thr Gly Phe Met Ser Ile Met His Gln 
        355                 360                 365             


Lys Arg Thr Ala Met Phe Gln Asp Pro Gln Glu Arg Pro Arg Lys Leu 
    370                 375                 380                 


Pro Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile His Asp Ile Ile Leu 
385                 390                 395                 400 


Glu Cys Val Tyr Cys Lys Gln Gln Leu Glu Asp Glu Ile Asp Gly Pro 
                405                 410                 415     


Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val Thr Phe 
            420                 425                 430         


Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln Ser Thr His 
        435                 440                 445             


Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr Leu Gly Ile 
    450                 455                 460                 


Val Cys Pro Ile Cys Ser Gln Lys Pro Gly Thr Thr Leu Glu Gln Gln 
465                 470                 475                 480 


Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile Asn Cys Gln 
                485                 490                 495     


Lys Pro Leu Cys Pro Glu Glu Lys Gln Arg His Leu Asp Lys Lys Gln 
            500                 505                 510         


Arg Phe His Asn Ile Arg Gly Arg Trp Thr Gly Arg Cys Met Ser Cys 
        515                 520                 525             


Cys Arg Ser Ser Arg Thr Arg Arg Glu Thr Gln Met His Gly Asp Thr 
    530                 535                 540                 


Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp 
545                 550                 555                 560 


Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser Glu Glu Glu Asp Glu 
                565                 570                 575     


Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn 
            580                 585                 590         


Ile Val Thr Phe Cys Cys Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile 
        595                 600                 605             


His Asp Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln Leu Leu Arg 
    610                 615                 620                 


Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp Leu Cys Ile Val Tyr Arg 
625                 630                 635                 640 


Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser 
                645                 650                 655     


Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr 
            660                 665                 670         


Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys 
        675                 680                 685             


Ile Asn Cys Gln Lys 
    690             


<210>  4
<211>  2085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding HPV16 E2E6E7SH designer polypeptide

<400>  4
atggaaaccc tgtgccagcg gctgaacgtg tgccaggaca agatcctgac ccactacgag       60

aacgacagca ccgacctgcg ggaccacatc gactactgga agcacatgcg gctggaatgc      120

gccatctact acaaggccag agagatgggc ttcaagcaca tcaaccacca ggtggtgccc      180

accctggccg tgtccaagaa caaggccctg caggccatcg agctgcagct gaccctggaa      240

accatctaca acagccagta cagcaacgag aagtggaccc tgcaggacgt gtccctggaa      300

gtgtacctga ccgctcccac cggctgcatc aagaaacacg gctacaccgt ggaagtgcag      360

ttcgacggcg acatctgcaa caccatgcac tacaccaact ggacccacat ctacatctgc      420

gaagaggcca gcgtgaccgt ggtggaaggc caggtggact actacggcct gtactacgtg      480

cacgagggca tccggaccta cttcgtgcag ttcaaggacg acgccgagaa gtacagcaag      540

aacaaagtgt gggaggtgca cgctggcggc caggtcatcc tgtgccccac cagcgtgttc      600

agcagcaacg aggtgtccag ccccgagatc atccggcagc acctggccaa tcaccctgcc      660

gccacccaca caaaggccgt ggccctgggc accgaggaaa cccagaccac catccagcgg      720

cccagaagcg agcccgacac cggcaatccc tgccacacca ccaagctgct gcaccgggac      780

agcgtggaca gcgcccctat cctgaccgcc ttcaacagca gccacaaggg ccggatcaac      840

tgcaacagca acaccacccc catcgtgcac ctgaaggtgg acgccaacac cctgatgcgg      900

ctgcggtaca gattcaagaa gcactgcacc ctgtacaccg ccgtgtcctc cacctggcac      960

tggaccggcc acaacgtgaa gcacaagagc gccatcgtga ccctgaccta cgacagcgag     1020

tggcagcggg accagttcct gagccaggtc aaaatcccca agaccatcac cgtgtccacc     1080

ggcttcatga gcatcatgca ccagaaacgg accgccatgt tccaggaccc ccaggaacgg     1140

cccagaaagc tgccccagct gtgcaccgag ctgcagacca ccatccacga catcatcctg     1200

gaatgcgtgt actgcaagca gcagctggaa gatgagatcg acggccctgc tggccaggcc     1260

gaacccgaca gagcccacta caatatcgtg accttctgct gcaagtgcga cagcaccctg     1320

cggctgtgcg tgcagagcac ccacgtggac atccggaccc tggaagatct gctgatgggc     1380

accctgggca tcgtgtgccc catctgcagc cagaagcccg gcaccaccct ggaacagcag     1440

tacaacaagc ccctgtgcga cctgctgatc cggtgcatca actgccagaa acccctgtgc     1500

cccgaggaaa agcagcggca cctggacaag aagcagcggt tccacaacat ccggggcaga     1560

tggacaggca gatgcatgag ctgctgcaga agcagccgga ccagacggga aacccagatg     1620

cacggcgaca cccccaccct gcacgagtac atgctggacc tgcagcccga gacaaccgac     1680

ctgtactgct acgagcagct gaacgacagc agcgaggaag aggacgagat tgacggaccc     1740

gctggacagg ccgagcctga ccgggctcac tataacatcg tgacattttg ctgtcagctc     1800

tgtactgaac tccagacaac aattcacgat attattctcg aatgtgtgta ttgtaaacag     1860

cagctcctgc ggagagaggt gtacgacttc gccttccggg acctctgcat cgtgtatcgg     1920

gacggcaacc cctacgccgt gtgcgacaag tgcctgaagt tctacagcaa gatcagcgag     1980

taccggcact actgctacag cctgtacgga acaacactcg aacagcagta taacaaacca     2040

ctctgtgatc tgctgattcg ctgtatcaat tgtcagaagt gataa                     2085


<210>  5
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPV16 E6E7E2SH designer polypeptide

<400>  5

Met His Gln Lys Arg Thr Ala Met Phe Gln Asp Pro Gln Glu Arg Pro 
1               5                   10                  15      


Arg Lys Leu Pro Gln Leu Cys Thr Glu Leu Gln Thr Thr Ile His Asp 
            20                  25                  30          


Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln Leu Glu Asp Glu Ile 
        35                  40                  45              


Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile 
    50                  55                  60                  


Val Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln 
65                  70                  75                  80  


Ser Thr His Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr 
                85                  90                  95      


Leu Gly Ile Val Cys Pro Ile Cys Ser Gln Lys Pro Gly Thr Thr Leu 
            100                 105                 110         


Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile 
        115                 120                 125             


Asn Cys Gln Lys Pro Leu Cys Pro Glu Glu Lys Gln Arg His Leu Asp 
    130                 135                 140                 


Lys Lys Gln Arg Phe His Asn Ile Arg Gly Arg Trp Thr Gly Arg Cys 
145                 150                 155                 160 


Met Ser Cys Cys Arg Ser Ser Arg Thr Arg Arg Glu Thr Gln Met His 
                165                 170                 175     


Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro Glu 
            180                 185                 190         


Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser Glu Glu 
        195                 200                 205             


Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala 
    210                 215                 220                 


His Tyr Asn Ile Val Thr Phe Cys Cys Gln Leu Cys Thr Glu Leu Gln 
225                 230                 235                 240 


Thr Thr Ile His Asp Ile Ile Leu Glu Cys Val Tyr Cys Lys Gln Gln 
                245                 250                 255     


Leu Leu Arg Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp Leu Cys Ile 
            260                 265                 270         


Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys 
        275                 280                 285             


Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr 
    290                 295                 300                 


Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu 
305                 310                 315                 320 


Ile Arg Cys Ile Asn Cys Gln Lys Met Glu Thr Leu Cys Gln Arg Leu 
                325                 330                 335     


Asn Val Cys Gln Asp Lys Ile Leu Thr His Tyr Glu Asn Asp Ser Thr 
            340                 345                 350         


Asp Leu Arg Asp His Ile Asp Tyr Trp Lys His Met Arg Leu Glu Cys 
        355                 360                 365             


Ala Ile Tyr Tyr Lys Ala Arg Glu Met Gly Phe Lys His Ile Asn His 
    370                 375                 380                 


Gln Val Val Pro Thr Leu Ala Val Ser Lys Asn Lys Ala Leu Gln Ala 
385                 390                 395                 400 


Ile Glu Leu Gln Leu Thr Leu Glu Thr Ile Tyr Asn Ser Gln Tyr Ser 
                405                 410                 415     


Asn Glu Lys Trp Thr Leu Gln Asp Val Ser Leu Glu Val Tyr Leu Thr 
            420                 425                 430         


Ala Pro Thr Gly Cys Ile Lys Lys His Gly Tyr Thr Val Glu Val Gln 
        435                 440                 445             


Phe Asp Gly Asp Ile Cys Asn Thr Met His Tyr Thr Asn Trp Thr His 
    450                 455                 460                 


Ile Tyr Ile Cys Glu Glu Ala Ser Val Thr Val Val Glu Gly Gln Val 
465                 470                 475                 480 


Asp Tyr Tyr Gly Leu Tyr Tyr Val His Glu Gly Ile Arg Thr Tyr Phe 
                485                 490                 495     


Val Gln Phe Lys Asp Asp Ala Glu Lys Tyr Ser Lys Asn Lys Val Trp 
            500                 505                 510         


Glu Val His Ala Gly Gly Gln Val Ile Leu Cys Pro Thr Ser Val Phe 
        515                 520                 525             


Ser Ser Asn Glu Val Ser Ser Pro Glu Ile Ile Arg Gln His Leu Ala 
    530                 535                 540                 


Asn His Pro Ala Ala Thr His Thr Lys Ala Val Ala Leu Gly Thr Glu 
545                 550                 555                 560 


Glu Thr Gln Thr Thr Ile Gln Arg Pro Arg Ser Glu Pro Asp Thr Gly 
                565                 570                 575     


Asn Pro Cys His Thr Thr Lys Leu Leu His Arg Asp Ser Val Asp Ser 
            580                 585                 590         


Ala Pro Ile Leu Thr Ala Phe Asn Ser Ser His Lys Gly Arg Ile Asn 
        595                 600                 605             


Cys Asn Ser Asn Thr Thr Pro Ile Val His Leu Lys Val Asp Ala Asn 
    610                 615                 620                 


Thr Leu Met Arg Leu Arg Tyr Arg Phe Lys Lys His Cys Thr Leu Tyr 
625                 630                 635                 640 


Thr Ala Val Ser Ser Thr Trp His Trp Thr Gly His Asn Val Lys His 
                645                 650                 655     


Lys Ser Ala Ile Val Thr Leu Thr Tyr Asp Ser Glu Trp Gln Arg Asp 
            660                 665                 670         


Gln Phe Leu Ser Gln Val Lys Ile Pro Lys Thr Ile Thr Val Ser Thr 
        675                 680                 685             


Gly Phe Met Ser Ile 
    690             


<210>  6
<211>  2085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding HPV16 E6E7E2SH designer polypeptide

<400>  6
atgcaccaga aacggaccgc catgttccag gacccccagg aacggcccag aaagctgccc       60

cagctgtgca ccgagctgca gaccaccatc cacgacatca tcctggaatg cgtgtactgc      120

aagcagcagc tggaagatga gatcgacggc cctgctggcc aggccgaacc cgacagagcc      180

cactacaata tcgtgacctt ctgctgcaag tgcgacagca ccctgcggct gtgcgtgcag      240

agcacccacg tggacatccg gaccctggaa gatctgctga tgggcaccct gggcatcgtg      300

tgccccatct gcagccagaa gcccggcacc accctggaac agcagtacaa caagcccctg      360

tgcgacctgc tgatccggtg catcaactgc cagaaacccc tgtgccccga ggaaaagcag      420

cggcacctgg acaagaagca gcggttccac aacatccggg gcagatggac aggcagatgc      480

atgagctgct gcagaagcag ccggaccaga cgggaaaccc agatgcacgg cgacaccccc      540

accctgcacg agtacatgct ggacctgcag cccgagacaa ccgacctgta ctgctacgag      600

cagctgaacg acagcagcga ggaagaggac gagattgacg gacccgctgg acaggccgag      660

cctgaccggg ctcactataa catcgtgaca ttttgctgtc agctctgtac tgaactccag      720

acaacaattc acgatattat tctcgaatgt gtgtattgta aacagcagct cctgcggaga      780

gaggtgtacg acttcgcctt ccgggacctc tgcatcgtgt atcgggacgg caacccctac      840

gccgtgtgcg acaagtgcct gaagttctac agcaagatca gcgagtaccg gcactactgc      900

tacagcctgt acggaacaac actcgaacag cagtataaca aaccactctg tgatctgctg      960

attcgctgta tcaattgtca gaagatggaa accctgtgcc agcggctgaa cgtgtgccag     1020

gacaagatcc tgacccacta cgagaacgac agcaccgacc tgcgggacca catcgactac     1080

tggaagcaca tgcggctgga atgcgccatc tactacaagg ccagagagat gggcttcaag     1140

cacatcaacc accaggtggt gcccaccctg gccgtgtcca agaacaaggc cctgcaggcc     1200

atcgagctgc agctgaccct ggaaaccatc tacaacagcc agtacagcaa cgagaagtgg     1260

accctgcagg acgtgtccct ggaagtgtac ctgaccgctc ccaccggctg catcaagaaa     1320

cacggctaca ccgtggaagt gcagttcgac ggcgacatct gcaacaccat gcactacacc     1380

aactggaccc acatctacat ctgcgaagag gccagcgtga ccgtggtgga aggccaggtg     1440

gactactacg gcctgtacta cgtgcacgag ggcatccgga cctacttcgt gcagttcaag     1500

gacgacgccg agaagtacag caagaacaaa gtgtgggagg tgcacgctgg cggccaggtc     1560

atcctgtgcc ccaccagcgt gttcagcagc aacgaggtgt ccagccccga gatcatccgg     1620

cagcacctgg ccaatcaccc tgccgccacc cacacaaagg ccgtggccct gggcaccgag     1680

gaaacccaga ccaccatcca gcggcccaga agcgagcccg acaccggcaa tccctgccac     1740

accaccaagc tgctgcaccg ggacagcgtg gacagcgccc ctatcctgac cgccttcaac     1800

agcagccaca agggccggat caactgcaac agcaacacca cccccatcgt gcacctgaag     1860

gtggacgcca acaccctgat gcggctgcgg tacagattca agaagcactg caccctgtac     1920

accgccgtgt cctccacctg gcactggacc ggccacaacg tgaagcacaa gagcgccatc     1980

gtgaccctga cctacgacag cgagtggcag cgggaccagt tcctgagcca ggtcaaaatc     2040

cccaagacca tcaccgtgtc caccggcttc atgagcatct gataa                     2085


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader peptide

<400>  7

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding IgE leader peptide

<400>  8
atggactgga cctggatcct gttcctggtg gctgccgcaa cccgggtgca cagc             54


<210>  9
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HAVT20 leader peptide

<400>  9

Met Ala Cys Pro Gly Phe Leu Trp Ala Leu Val Ile Ser Thr Cys Leu 
1               5                   10                  15      


Glu Phe Ser Met Ala 
            20      


<210>  10
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding HAVT20 leader peptide

<400>  10
atggcctgcc ccggctttct gtgggccctg gtcatcagca cctgtctgga attcagcatg       60

gcc                                                                     63


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2xTetO-containing sequence

<400>  11
gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgac             54


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CuO-containing sequence

<400>  12
aacaaacaga caatctggtc tgtttgta                                          28


<210>  13
<211>  829
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV promoter

<400>  13
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc      120

aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc      660

cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      720

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag      780

aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattgga                  829


<210>  14
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding TetR polypeptide

<400>  14
atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc       60

ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca      120

ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta      180

gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt      240

aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat      300

ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta      360

tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt      420

actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca      480

cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa      540

ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa      600

cttaaatgtg aaagtgggtc cgcgtacagc ggatcccggg aattcagatc ttattaa         657


<210>  15
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TetR polypeptide

<400>  15

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 


Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 


Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     


Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         


Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser Ala 
        195                 200                 205             


Tyr Ser Gly Ser Arg Glu Phe Arg Ser Tyr 
    210                 215             


<210>  16
<211>  612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nt sequence encoding CymR polypeptide

<400>  16
atgtctccca aacgacggac tcaagcggaa agggcaatgg aaactcaggg taagctgatt       60

gccgcggctc tgggagtgct gcgagagaaa gggtatgccg ggtttcgcat agccgacgtt      120

cctggagctg caggcgtaag cagaggagcc caatctcatc actttccgac caagctggag      180

cttttgctgg ctaccttcga atggctgtac gagcagatca cggaaaggag tcgtgctagg      240

ctggccaagc tgaaacccga ggatgatgtc attcagcaga tgctggacga tgcagccgag      300

ttcttcctgg acgacgactt cagcatcagt ctcgacctca tcgtagccgc agatcgcgat      360

ccagctttgc gcgagggcat acagagaaca gtcgagcgga atcggtttgt ggtggaggac      420

atgtggcttg gtgttctggt gagcagaggc ctctcacggg atgatgccga ggacatcctg      480

tggctgatct ttaactccgt cagagggttg gcagtgaggt ccctttggca gaaggacaaa      540

gaacggtttg aacgtgtgcg aaactcaaca ctcgagattg ctagggaacg ctacgccaag      600

ttcaagagat ga                                                          612


<210>  17
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CymR polypeptide

<400>  17

Met Ser Pro Lys Arg Arg Thr Gln Ala Glu Arg Ala Met Glu Thr Gln 
1               5                   10                  15      


Gly Lys Leu Ile Ala Ala Ala Leu Gly Val Leu Arg Glu Lys Gly Tyr 
            20                  25                  30          


Ala Gly Phe Arg Ile Ala Asp Val Pro Gly Ala Ala Gly Val Ser Arg 
        35                  40                  45              


Gly Ala Gln Ser His His Phe Pro Thr Lys Leu Glu Leu Leu Leu Ala 
    50                  55                  60                  


Thr Phe Glu Trp Leu Tyr Glu Gln Ile Thr Glu Arg Ser Arg Ala Arg 
65                  70                  75                  80  


Leu Ala Lys Leu Lys Pro Glu Asp Asp Val Ile Gln Gln Met Leu Asp 
                85                  90                  95      


Asp Ala Ala Glu Phe Phe Leu Asp Asp Asp Phe Ser Ile Ser Leu Asp 
            100                 105                 110         


Leu Ile Val Ala Ala Asp Arg Asp Pro Ala Leu Arg Glu Gly Ile Gln 
        115                 120                 125             


Arg Thr Val Glu Arg Asn Arg Phe Val Val Glu Asp Met Trp Leu Gly 
    130                 135                 140                 


Val Leu Val Ser Arg Gly Leu Ser Arg Asp Asp Ala Glu Asp Ile Leu 
145                 150                 155                 160 


Trp Leu Ile Phe Asn Ser Val Arg Gly Leu Ala Val Arg Ser Leu Trp 
                165                 170                 175     


Gln Lys Asp Lys Glu Arg Phe Glu Arg Val Arg Asn Ser Thr Leu Glu 
            180                 185                 190         


Ile Ala Arg Glu Arg Tyr Ala Lys Phe Lys Arg 
        195                 200             


<210>  18
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPV16 E6 aa41-65

<400>  18

Lys Gln Gln Leu Leu Arg Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp 
1               5                   10                  15      


Leu Cys Ile Val Tyr Arg Asp Gly Asn 
            20                  25  


<210>  19
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPV16 E7 aa43-77

<400>  19

Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val Thr Phe Cys 
1               5                   10                  15      


Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln Ser Thr His Val 
            20                  25                  30          


Asp Ile Arg 
        35  


