                               SEQUENCE LISTING



<110> GENIA TECHNOLOGIES, INC.

 

<120> ALPHA-HEMOLYSIN VARIANTS WITH ALTERED CHARACTERISTICS



<130> 20-04338.P519WO1



<140>

<141>



<150> 62/073,936

<151> 2014-10-31



<160> 8     



<170> PatentIn version 3.5



<210> 1

<211> 1023

<212> DNA

<213> Staphylococcus aureus



<400> 1

atggcagatc tcgatcccgc gaaattaata cgactcacta tagggaggcc acaacggttt       60



ccctctagaa ataattttgt ttaactttaa gaaggagata tacaaatgga ttcagatatt      120



aatattaaaa caggtacaac agatattggt tcaaatacaa cagtaaaaac tggtgattta      180



gtaacttatg ataaagaaaa tggtatgcat aaaaaagtat tttattcttt tattgatgat      240



aaaaatcata ataaaaaatt gttagttatt cgtacaaaag gtactattgc aggtcaatat      300



agagtatata gtgaagaagg tgctaataaa agtggtttag catggccatc tgcttttaaa      360



gttcaattac aattacctga taatgaagta gcacaaattt cagattatta tccacgtaat      420



agtattgata caaaagaata tatgtcaaca ttaacttatg gttttaatgg taatgtaaca      480



ggtgatgata ctggtaaaat tggtggttta attggtgcta atgtttcaat tggtcataca      540



ttaaaatatg tacaaccaga ttttaaaaca attttagaaa gtcctactga taaaaaagtt      600



ggttggaaag taatttttaa taatatggtt aatcaaaatt ggggtcctta tgatcgtgat      660



agttggaatc ctgtatatgg taatcaatta tttatgaaaa caagaaatgg ttctatgaaa      720



gcagctgata atttcttaga tccaaataaa gcatcaagtt tattatcttc aggtttttct      780



cctgattttg caacagttat tactatggat agaaaagcat caaaacaaca aacaaatatt      840



gatgttattt atgaacgtgt aagagatgat tatcaattac attggacatc aactaattgg      900



aaaggtacaa atactaaaga taaatggaca gatagaagtt cagaaagata taaaattgat      960



tgggaaaaag aagaaatgac aaatggtctc agcgcttgga gccacccgca gttcgaaaaa     1020



taa                                                                   1023





<210> 2

<211> 306

<212> PRT

<213> Staphylococcus aureus



<400> 2

Met Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly 

1               5                   10                  15      





Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu 

            20                  25                  30          





Asn Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn 

        35                  40                  45              





His Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly 

    50                  55                  60                  





Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala 

65                  70                  75                  80  





Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val 

                85                  90                  95      





Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu 

            100                 105                 110         





Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp 

        115                 120                 125             





Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly 

    130                 135                 140                 





His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser 

145                 150                 155                 160 





Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val 

                165                 170                 175     





Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr 

            180                 185                 190         





Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala 

        195                 200                 205             





Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly 

    210                 215                 220                 





Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser 

225                 230                 235                 240 





Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp 

                245                 250                 255     





Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys 

            260                 265                 270         





Asp Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu 

        275                 280                 285             





Lys Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe 

    290                 295                 300                 





Glu Lys 

305     





<210> 3

<211> 305

<212> PRT

<213> Staphylococcus aureus



<400> 3

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 

1               5                   10                  15      





Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 

    290                 295                 300                 





Lys 

305 





<210> 4

<211> 305

<212> PRT

<213> Staphylococcus aureus



<400> 4

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 

1               5                   10                  15      





Lys Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 

    290                 295                 300                 





Lys 

305 





<210> 5

<211> 305

<212> PRT

<213> Staphylococcus aureus



<400> 5

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 

1               5                   10                  15      





Arg Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 

    290                 295                 300                 





Lys 

305 





<210> 6

<211> 305

<212> PRT

<213> Staphylococcus aureus



<400> 6

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Lys Asp Ile Gly Ser 

1               5                   10                  15      





Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 

    290                 295                 300                 





Lys 

305 





<210> 7

<211> 305

<212> PRT

<213> Staphylococcus aureus



<400> 7

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Arg Asp Ile Gly Ser 

1               5                   10                  15      





Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 

    290                 295                 300                 





Lys 

305 





<210> 8

<211> 293

<212> PRT

<213> Staphylococcus aureus



<400> 8

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 

1               5                   10                  15      





Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 

            20                  25                  30          





Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 

        35                  40                  45              





Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 

    50                  55                  60                  





Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 

65                  70                  75                  80  





Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 

                85                  90                  95      





Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 

            100                 105                 110         





Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 

        115                 120                 125             





Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 

    130                 135                 140                 





Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 

145                 150                 155                 160 





Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 

                165                 170                 175     





Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 

            180                 185                 190         





Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 

        195                 200                 205             





Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 

    210                 215                 220                 





Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 

225                 230                 235                 240 





Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 

                245                 250                 255     





Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 

            260                 265                 270         





Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 

        275                 280                 285             





Glu Glu Met Thr Asn 

    290             





