                         SEQUENCE LISTING

<110>  Celgene Corporation
       Washington University
 
<120>  TREATMENT OF CARDIOVASCULAR DISEASE USING ACTRII LIGAND TRAPS

<130>  12827-934-228

<150>  62/062,021
<151>  2014-10-09

<150>  62/078,321
<151>  2014-11-11

<150>  62/103,515
<151>  2015-01-14

<150>  62/167,052
<151>  2015-05-27

<150>  62/170,015
<151>  2015-06-02

<160>  87    

<170>  PatentIn version 3.5

<210>  1
<211>  513
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human ActRIIA precursor polypeptide

<400>  1

Met Gly Ala Ala Ala Lys Leu Ala Phe Ala Val Phe Leu Ile Ser Cys 
1               5                   10                  15      


Ser Ser Gly Ala Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe 
            20                  25                  30          


Phe Asn Ala Asn Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu 
        35                  40                  45              


Pro Cys Tyr Gly Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp 
    50                  55                  60                  


Lys Asn Ile Ser Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu 
65                  70                  75                  80  


Asp Asp Ile Asn Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp 
                85                  90                  95      


Ser Pro Glu Val Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu 
            100                 105                 110         


Lys Phe Ser Tyr Phe Pro Glu Met Glu Val Thr Gln Pro Thr Ser Asn 
        115                 120                 125             


Pro Val Thr Pro Lys Pro Pro Tyr Tyr Asn Ile Leu Leu Tyr Ser Leu 
    130                 135                 140                 


Val Pro Leu Met Leu Ile Ala Gly Ile Val Ile Cys Ala Phe Trp Val 
145                 150                 155                 160 


Tyr Arg His His Lys Met Ala Tyr Pro Pro Val Leu Val Pro Thr Gln 
                165                 170                 175     


Asp Pro Gly Pro Pro Pro Pro Ser Pro Leu Leu Gly Leu Lys Pro Leu 
            180                 185                 190         


Gln Leu Leu Glu Val Lys Ala Arg Gly Arg Phe Gly Cys Val Trp Lys 
        195                 200                 205             


Ala Gln Leu Leu Asn Glu Tyr Val Ala Val Lys Ile Phe Pro Ile Gln 
    210                 215                 220                 


Asp Lys Gln Ser Trp Gln Asn Glu Tyr Glu Val Tyr Ser Leu Pro Gly 
225                 230                 235                 240 


Met Lys His Glu Asn Ile Leu Gln Phe Ile Gly Ala Glu Lys Arg Gly 
                245                 250                 255     


Thr Ser Val Asp Val Asp Leu Trp Leu Ile Thr Ala Phe His Glu Lys 
            260                 265                 270         


Gly Ser Leu Ser Asp Phe Leu Lys Ala Asn Val Val Ser Trp Asn Glu 
        275                 280                 285             


Leu Cys His Ile Ala Glu Thr Met Ala Arg Gly Leu Ala Tyr Leu His 
    290                 295                 300                 


Glu Asp Ile Pro Gly Leu Lys Asp Gly His Lys Pro Ala Ile Ser His 
305                 310                 315                 320 


Arg Asp Ile Lys Ser Lys Asn Val Leu Leu Lys Asn Asn Leu Thr Ala 
                325                 330                 335     


Cys Ile Ala Asp Phe Gly Leu Ala Leu Lys Phe Glu Ala Gly Lys Ser 
            340                 345                 350         


Ala Gly Asp Thr His Gly Gln Val Gly Thr Arg Arg Tyr Met Ala Pro 
        355                 360                 365             


Glu Val Leu Glu Gly Ala Ile Asn Phe Gln Arg Asp Ala Phe Leu Arg 
    370                 375                 380                 


Ile Asp Met Tyr Ala Met Gly Leu Val Leu Trp Glu Leu Ala Ser Arg 
385                 390                 395                 400 


Cys Thr Ala Ala Asp Gly Pro Val Asp Glu Tyr Met Leu Pro Phe Glu 
                405                 410                 415     


Glu Glu Ile Gly Gln His Pro Ser Leu Glu Asp Met Gln Glu Val Val 
            420                 425                 430         


Val His Lys Lys Lys Arg Pro Val Leu Arg Asp Tyr Trp Gln Lys His 
        435                 440                 445             


Ala Gly Met Ala Met Leu Cys Glu Thr Ile Glu Glu Cys Trp Asp His 
    450                 455                 460                 


Asp Ala Glu Ala Arg Leu Ser Ala Gly Cys Val Gly Glu Arg Ile Thr 
465                 470                 475                 480 


Gln Met Gln Arg Leu Thr Asn Ile Ile Thr Thr Glu Asp Ile Val Thr 
                485                 490                 495     


Val Val Thr Met Val Thr Asn Val Asp Phe Pro Pro Lys Glu Ser Ser 
            500                 505                 510         


Leu 
    


<210>  2
<211>  115
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human ActRIIA soluble (extracellular), processed polypeptide 
       sequence

<400>  2

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met Glu Val Thr Gln Pro Thr Ser Asn Pro Val Thr Pro 
            100                 105                 110         


Lys Pro Pro 
        115 


<210>  3
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIA soluble (extracellular), processed polypeptide 
       sequence with the C-terminal 15 amino acids deleted

<400>  3

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met 
            100 


<210>  4
<211>  1542
<212>  DNA
<213>  Homo sapiens

<220>
<223>  nucleic acid sequence encoding human ActRIIA precursor protein

<400>  4
atgggagctg ctgcaaagtt ggcgtttgcc gtctttctta tctcctgttc ttcaggtgct       60

atacttggta gatcagaaac tcaggagtgt cttttcttta atgctaattg ggaaaaagac      120

agaaccaatc aaactggtgt tgaaccgtgt tatggtgaca aagataaacg gcggcattgt      180

tttgctacct ggaagaatat ttctggttcc attgaaatag tgaaacaagg ttgttggctg      240

gatgatatca actgctatga caggactgat tgtgtagaaa aaaaagacag ccctgaagta      300

tatttttgtt gctgtgaggg caatatgtgt aatgaaaagt tttcttattt tccagagatg      360

gaagtcacac agcccacttc aaatccagtt acacctaagc caccctatta caacatcctg      420

ctctattcct tggtgccact tatgttaatt gcggggattg tcatttgtgc attttgggtg      480

tacaggcatc acaagatggc ctaccctcct gtacttgttc caactcaaga cccaggacca      540

cccccacctt ctccattact agggttgaaa ccactgcagt tattagaagt gaaagcaagg      600

ggaagatttg gttgtgtctg gaaagcccag ttgcttaacg aatatgtggc tgtcaaaata      660

tttccaatac aggacaaaca gtcatggcaa aatgaatacg aagtctacag tttgcctgga      720

atgaagcatg agaacatatt acagttcatt ggtgcagaaa aacgaggcac cagtgttgat      780

gtggatcttt ggctgatcac agcatttcat gaaaagggtt cactatcaga ctttcttaag      840

gctaatgtgg tctcttggaa tgaactgtgt catattgcag aaaccatggc tagaggattg      900

gcatatttac atgaggatat acctggccta aaagatggcc acaaacctgc catatctcac      960

agggacatca aaagtaaaaa tgtgctgttg aaaaacaacc tgacagcttg cattgctgac     1020

tttgggttgg ccttaaaatt tgaggctggc aagtctgcag gcgataccca tggacaggtt     1080

ggtacccgga ggtacatggc tccagaggta ttagagggtg ctataaactt cgaaagggat     1140

gcatttttga ggatagatat gtatgccatg ggattagtcc tatgggaact ggcttctcgc     1200

tgtactgctg cagatggacc tgtagatgaa tacatgttgc catttgagga ggaaattggc     1260

cagcatccat ctcttgaaga catgcaggaa gttgttgtgc ataaaaaaaa gaggcctgtt     1320

ttaagagatt attggcagaa acatgctgga atggcaatgc tctgtgaaac cattgaagaa     1380

tgttgggatc acgacgcaga agccaggtta tcagctggat gtgtaggtga aagaattacc     1440

cagatgcaga gactaacaaa tattattacc acagaggaca ttgtaacagt ggtcacaatg     1500

gtgacaaatg ttgactttcc tcccaaagaa tctagtctat ga                        1542


<210>  5
<211>  345
<212>  DNA
<213>  Homo sapiens

<220>
<223>  nucleic acid sequence encoding a human ActRIIA soluble 
       (extracellular) polypeptide

<400>  5
atacttggta gatcagaaac tcaggagtgt cttttcttta atgctaattg ggaaaaagac       60

agaaccaatc aaactggtgt tgaaccgtgt tatggtgaca aagataaacg gcggcattgt      120

tttgctacct ggaagaatat ttctggttcc attgaaatag tgaaacaagg ttgttggctg      180

gatgatatca actgctatga caggactgat tgtgtagaaa aaaaagacag ccctgaagta      240

tatttttgtt gctgtgaggg caatatgtgt aatgaaaagt tttcttattt tccagagatg      300

gaagtcacac agcccacttc aaatccagtt acacctaagc caccc                      345


<210>  6
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising a soluble extracellular domain of 
       ActRIIA fused to an Fc domain


<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa = Asp or Ala

<220>
<221>  misc_feature
<222>  (102)..(102)
<223>  Xaa = Lys or Ala

<220>
<221>  misc_feature
<222>  (215)..(215)
<223>  Xaa = Asn or Ala

<400>  6

Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
1               5                   10                  15      


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
            20                  25                  30          


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Xaa Val Ser His Glu 
        35                  40                  45              


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    50                  55                  60                  


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
65                  70                  75                  80  


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                85                  90                  95      


Glu Tyr Lys Cys Lys Xaa Val Ser Asn Lys Ala Leu Pro Val Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Pro Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn Xaa His Tyr Thr Gln Lys Ser Leu Ser Leu 
    210                 215                 220                 


Ser Pro Gly Lys 
225             


<210>  7
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of human ActRIIA fused to a human Fc domain

<400>  7

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met Glu Val Thr Gln Pro Thr Ser Asn Pro Val Thr Pro 
            100                 105                 110         


Lys Pro Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
        115                 120                 125             


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    130                 135                 140                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
145                 150                 155                 160 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                165                 170                 175     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            180                 185                 190         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        195                 200                 205             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    210                 215                 220                 


Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
225                 230                 235                 240 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
                245                 250                 255     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            260                 265                 270         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        275                 280                 285             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    290                 295                 300                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
305                 310                 315                 320 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                325                 330                 335     


Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader sequence of Honey bee mellitin (HBML)

<400>  8

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala 
            20      


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader sequence of Tissue Plasminogen Activator (TPA)

<400>  9

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro 
            20          


<210>  10
<211>  20
<212>  PRT
<213>  Unknown

<220>
<223>  Native ActRIIA leader

<400>  10

Met Gly Ala Ala Ala Lys Leu Ala Phe Ala Val Phe Leu Ile Ser Cys 
1               5                   10                  15      


Ser Ser Gly Ala 
            20  


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ActRIIA-hFc and mActRIIA-Fc N-terminal sequence

<400>  11

Ile Leu Gly Arg Ser Glu Thr Gln Glu 
1               5                   


<210>  12
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ActRIIA-Fc Protein with deletion of the C-terminal 15 amino acids
       of the extracellular domain of ActRIIA

<400>  12

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro 
            100                 105                 110         


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
        115                 120                 125             


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
    130                 135                 140                 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
145                 150                 155                 160 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
                165                 170                 175     


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
            180                 185                 190         


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
        195                 200                 205             


Ala Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
    210                 215                 220                 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
225                 230                 235                 240 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
                245                 250                 255     


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
            260                 265                 270         


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
    290                 295                 300                 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 


<210>  13
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unprocessed ActRIIA-hFc with TPA leader sequence

<400>  13

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Gly Ala Ala Ile Leu Gly Arg Ser Glu Thr 
            20                  25                  30          


Gln Glu Cys Leu Phe Phe Asn Ala Asn Trp Glu Lys Asp Arg Thr Asn 
        35                  40                  45              


Gln Thr Gly Val Glu Pro Cys Tyr Gly Asp Lys Asp Lys Arg Arg His 
    50                  55                  60                  


Cys Phe Ala Thr Trp Lys Asn Ile Ser Gly Ser Ile Glu Ile Val Lys 
65                  70                  75                  80  


Gln Gly Cys Trp Leu Asp Asp Ile Asn Cys Tyr Asp Arg Thr Asp Cys 
                85                  90                  95      


Val Glu Lys Lys Asp Ser Pro Glu Val Tyr Phe Cys Cys Cys Glu Gly 
            100                 105                 110         


Asn Met Cys Asn Glu Lys Phe Ser Tyr Phe Pro Glu Met Glu Val Thr 
        115                 120                 125             


Gln Pro Thr Ser Asn Pro Val Thr Pro Lys Pro Pro Thr Gly Gly Gly 
    130                 135                 140                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
145                 150                 155                 160 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                165                 170                 175     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            180                 185                 190         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        195                 200                 205             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    210                 215                 220                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
225                 230                 235                 240 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Val Pro Ile Glu Lys 
                245                 250                 255     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            260                 265                 270         


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        275                 280                 285             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    290                 295                 300                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
305                 310                 315                 320 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                325                 330                 335     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            340                 345                 350         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        355                 360                 365             


Lys 
    


<210>  14
<211>  1113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence encoding Unprocessed ActRIIA-hFc with TPA 
       leader sequence

<400>  14
atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt       60

tcgcccggcg ccgctatact tggtagatca gaaactcagg agtgtctttt tttaatgcta      120

attgggaaaa agacagaacc aatcaaactg gtgttgaacc gtgttatggt gacaaagata      180

aacggcggca ttgttttgct acctggaaga atatttctgg ttccattgaa tagtgaaaca      240

aggttgttgg ctggatgata tcaactgcta tgacaggact gattgtgtag aaaaaaaaga      300

cagccctgaa gtatatttct gttgctgtga gggcaatatg tgtaatgaaa agttttctta      360

ttttccggag atggaagtca cacagcccac ttcaaatcca gttacaccta agccacccac      420

cggtggtgga actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc      480

agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt      540

cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt      600

ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac      660

gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta      720

caagtgcaag gtctccaaca aagccctccc agtccccatc gagaaaacca tctccaaagc      780

caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg aggagatgac      840

caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt      900

ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga      960

ctccgacggc tccttcttcc tctatagcaa gctcaccgtg gacaagagca ggtggcagca     1020

ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa     1080

gagcctctcc ctgtctccgg taaatgagaa ttc                                  1113


<210>  15
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the N-terminal 6 amino acids of the EC domain 
       deleted and the C-terminal 4 amino acids of the EC domain deleted
       (amino acids 25-130 of SEQ ID NO:28) and with an L79D mutation

<400>  15

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105     


<210>  16
<211>  512
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human ActRIIB precursor protein sequence (A64)

<400>  16

Met Thr Ala Pro Trp Val Ala Leu Ala Leu Leu Trp Gly Ser Leu Trp 
1               5                   10                  15      


Pro Gly Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr 
            20                  25                  30          


Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg 
        35                  40                  45              


Cys Glu Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala 
    50                  55                  60                  


Asn Ser Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp 
65                  70                  75                  80  


Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn 
                85                  90                  95      


Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg 
            100                 105                 110         


Phe Thr His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro 
        115                 120                 125             


Pro Pro Thr Ala Pro Thr Leu Leu Thr Val Leu Ala Tyr Ser Leu Leu 
    130                 135                 140                 


Pro Ile Gly Gly Leu Ser Leu Ile Val Leu Leu Ala Phe Trp Met Tyr 
145                 150                 155                 160 


Arg His Arg Lys Pro Pro Tyr Gly His Val Asp Ile His Glu Asp Pro 
                165                 170                 175     


Gly Pro Pro Pro Pro Ser Pro Leu Val Gly Leu Lys Pro Leu Gln Leu 
            180                 185                 190         


Leu Glu Ile Lys Ala Arg Gly Arg Phe Gly Cys Val Trp Lys Ala Gln 
        195                 200                 205             


Leu Met Asn Asp Phe Val Ala Val Lys Ile Phe Pro Leu Gln Asp Lys 
    210                 215                 220                 


Gln Ser Trp Gln Ser Glu Arg Glu Ile Phe Ser Thr Pro Gly Met Lys 
225                 230                 235                 240 


His Glu Asn Leu Leu Gln Phe Ile Ala Ala Glu Lys Arg Gly Ser Asn 
                245                 250                 255     


Leu Glu Val Glu Leu Trp Leu Ile Thr Ala Phe His Asp Lys Gly Ser 
            260                 265                 270         


Leu Thr Asp Tyr Leu Lys Gly Asn Ile Ile Thr Trp Asn Glu Leu Cys 
        275                 280                 285             


His Val Ala Glu Thr Met Ser Arg Gly Leu Ser Tyr Leu His Glu Asp 
    290                 295                 300                 


Val Pro Trp Cys Arg Gly Glu Gly His Lys Pro Ser Ile Ala His Arg 
305                 310                 315                 320 


Asp Phe Lys Ser Lys Asn Val Leu Leu Lys Ser Asp Leu Thr Ala Val 
                325                 330                 335     


Leu Ala Asp Phe Gly Leu Ala Val Arg Phe Glu Pro Gly Lys Pro Pro 
            340                 345                 350         


Gly Asp Thr His Gly Gln Val Gly Thr Arg Arg Tyr Met Ala Pro Glu 
        355                 360                 365             


Val Leu Glu Gly Ala Ile Asn Phe Gln Arg Asp Ala Phe Leu Arg Ile 
    370                 375                 380                 


Asp Met Tyr Ala Met Gly Leu Val Leu Trp Glu Leu Val Ser Arg Cys 
385                 390                 395                 400 


Lys Ala Ala Asp Gly Pro Val Asp Glu Tyr Met Leu Pro Phe Glu Glu 
                405                 410                 415     


Glu Ile Gly Gln His Pro Ser Leu Glu Glu Leu Gln Glu Val Val Val 
            420                 425                 430         


His Lys Lys Met Arg Pro Thr Ile Lys Asp His Trp Leu Lys His Pro 
        435                 440                 445             


Gly Leu Ala Gln Leu Cys Val Thr Ile Glu Glu Cys Trp Asp His Asp 
    450                 455                 460                 


Ala Glu Ala Arg Leu Ser Ala Gly Cys Val Glu Glu Arg Val Ser Leu 
465                 470                 475                 480 


Ile Arg Arg Ser Val Asn Gly Thr Thr Ser Asp Cys Leu Val Ser Leu 
                485                 490                 495     


Val Thr Ser Val Thr Asn Val Asp Leu Pro Pro Lys Glu Ser Ser Ile 
            500                 505                 510         


<210>  17
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 19-134 of SEQ ID NO:16)

<400>  17

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105                 110         


Thr Ala Pro Thr 
        115     


<210>  18
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the C-terminal 15 amino acids deleted (amino acids 
       19-119 of SEQ ID NO:16)

<400>  18

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala 
            100     


<210>  19
<211>  1539
<212>  DNA
<213>  Homo sapiens

<220>
<223>  nucleic acid sequence encoding a human ActRIIB (A64) precursor 
       protein

<400>  19
atgacggcgc cctgggtggc cctcgccctc ctctggggat cgctgtggcc cggctctggg       60

cgtggggagg ctgagacacg ggagtgcatc tactacaacg ccaactggga gctggagcgc      120

accaaccaga gcggcctgga gcgctgcgaa ggcgagcagg acaagcggct gcactgctac      180

gcctcctggg ccaacagctc tggcaccatc gagctcgtga agaagggctg ctggctagat      240

gacttcaact gctacgatag gcaggagtgt gtggccactg aggagaaccc ccaggtgtac      300

ttctgctgct gtgaaggcaa cttctgcaac gagcgcttca ctcatttgcc agaggctggg      360

ggcccggaag tcacgtacga gccacccccg acagccccca ccctgctcac ggtgctggcc      420

tactcactgc tgcccatcgg gggcctttcc ctcatcgtcc tgctggcctt ttggatgtac      480

cggcatcgca agccccccta cggtcatgtg gacatccatg aggaccctgg gcctccacca      540

ccatcccctc tggtgggcct gaagccactg cagctgctgg agatcaaggc tcgggggcgc      600

tttggctgtg tctggaaggc ccagctcatg aatgactttg tagctgtcaa gatcttccca      660

ctccaggaca agcagtcgtg gcagagtgaa cgggagatct tcagcacacc tggcatgaag      720

cacgagaacc tgctacagtt cattgctgcc gagaagcgag gctccaacct cgaagtagag      780

ctgtggctca tcacggcctt ccatgacaag ggctccctca cggattacct caaggggaac      840

atcatcacat ggaacgaact gtgtcatgta gcagagacga tgtcacgagg cctctcatac      900

ctgcatgagg atgtgccctg gtgccgtggc gagggccaca agccgtctat tgcccacagg      960

gactttaaaa gtaagaatgt attgctgaag agcgacctca cagccgtgct ggctgacttt     1020

ggcttggctg ttcgatttga gccagggaaa cctccagggg acacccacgg acaggtaggc     1080

acgagacggt acatggctcc tgaggtgctc gagggagcca tcaacttcca gagagatgcc     1140

ttcctgcgca ttgacatgta tgccatgggg ttggtgctgt gggagcttgt gtctcgctgc     1200

aaggctgcag acggacccgt ggatgagtac atgctgccct ttgaggaaga gattggccag     1260

cacccttcgt tggaggagct gcaggaggtg gtggtgcaca agaagatgag gcccaccatt     1320

aaagatcact ggttgaaaca cccgggcctg gcccagcttt gtgtgaccat cgaggagtgc     1380

tgggaccatg atgcagaggc tcgcttgtcc gcgggctgtg tggaggagcg ggtgtccctg     1440

attcggaggt cggtcaacgg cactacctcg gactgtctcg tttccctggt gacctctgtc     1500

accaatgtgg acctgccccc taaagagtca agcatctaa                            1539


<210>  20
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising a soluble extracellular domain of 
       ActRIIB (A64; SEQ ID NO:17) fused to an Fc domain

<400>  20

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105                 110         


Thr Ala Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
        115                 120                 125             


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    130                 135                 140                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
145                 150                 155                 160 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                165                 170                 175     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            180                 185                 190         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        195                 200                 205             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    210                 215                 220                 


Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
225                 230                 235                 240 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
                245                 250                 255     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            260                 265                 270         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        275                 280                 285             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    290                 295                 300                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
305                 310                 315                 320 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                325                 330                 335     


Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 


<210>  21
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising a soluble extracellular domain of 
       ActRIIB (A64) with the C-terminal 15 amino acids deleted (SEQ ID 
       NO:18) fused to an Fc domain

<400>  21

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro 
            100                 105                 110         


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
        115                 120                 125             


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
    130                 135                 140                 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
145                 150                 155                 160 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
                165                 170                 175     


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
            180                 185                 190         


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
        195                 200                 205             


Ala Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
    210                 215                 220                 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
225                 230                 235                 240 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
                245                 250                 255     


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
            260                 265                 270         


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
    290                 295                 300                 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 


<210>  22
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the N-terminal 6 amino acids of the EC domain 
       deleted and the C-terminal 5 amino acids of the EC domain deleted
       (amino acids 25-129 of SEQ ID NO:28) and with an L79D mutation

<400>  22

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro 
            100                 105 


<210>  23
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the N-terminal 6 amino acids of the EC domain 
       deleted and the C-terminal 3 amino acids of the EC domain deleted
       (amino acids 25-131 of SEQ ID NO:28) and with an L79D mutation

<400>  23

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105         


<210>  24
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unprocessed ActRIIB-Fc fusion protein with the N-terminal 6 amino
       acids of the EC domain deleted and the C-terminal 3 amino acids 
       of the EC domain deleted (amino acids 25-131 of SEQ ID NO:28) and
       with an L79D mutation and with TPA leader sequence

<400>  24

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Gly Ala Ala Glu Thr Arg Glu Cys Ile Tyr 
            20                  25                  30          


Tyr Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu 
        35                  40                  45              


Arg Cys Glu Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp 
    50                  55                  60                  


Arg Asn Ser Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp 
65                  70                  75                  80  


Asp Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu 
                85                  90                  95      


Asn Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu 
            100                 105                 110         


Arg Phe Thr His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu 
        115                 120                 125             


Pro Pro Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
    130                 135                 140                 


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
145                 150                 155                 160 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
                165                 170                 175     


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
            180                 185                 190         


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
        195                 200                 205             


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
    210                 215                 220                 


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
225                 230                 235                 240 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
                245                 250                 255     


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
            260                 265                 270         


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
        275                 280                 285             


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
    290                 295                 300                 


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
305                 310                 315                 320 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
                325                 330                 335     


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
            340                 345                 350         


Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360 


<210>  25
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Processed ActRIIB-Fc fusion protein with the N-terminal 6 amino 
       acids of the EC domain deleted and the C-terminal 3 amino acids 
       of the EC domain deleted (amino acids 25-131 of SEQ ID NO:28) and
       with an L79D mutation

<400>  25

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr Gly Gly Gly Thr His 
            100                 105                 110         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        115                 120                 125             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    130                 135                 140                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
145                 150                 155                 160 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                165                 170                 175     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            180                 185                 190         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        195                 200                 205             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    210                 215                 220                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
225                 230                 235                 240 


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                245                 250                 255     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            260                 265                 270         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        275                 280                 285             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    290                 295                 300                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
305                 310                 315                 320 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330                 335 


<210>  26
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:16)

<400>  26

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr 
        115 


<210>  27
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the C-terminal 15 amino acids deleted (amino acids 
       20-119 of SEQ ID NO:16)

<400>  27

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala 
            100 


<210>  28
<211>  512
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human ActRIIB precursor protein sequence (R64)

<400>  28

Met Thr Ala Pro Trp Val Ala Leu Ala Leu Leu Trp Gly Ser Leu Trp 
1               5                   10                  15      


Pro Gly Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr 
            20                  25                  30          


Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg 
        35                  40                  45              


Cys Glu Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg 
    50                  55                  60                  


Asn Ser Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp 
65                  70                  75                  80  


Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn 
                85                  90                  95      


Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg 
            100                 105                 110         


Phe Thr His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro 
        115                 120                 125             


Pro Pro Thr Ala Pro Thr Leu Leu Thr Val Leu Ala Tyr Ser Leu Leu 
    130                 135                 140                 


Pro Ile Gly Gly Leu Ser Leu Ile Val Leu Leu Ala Phe Trp Met Tyr 
145                 150                 155                 160 


Arg His Arg Lys Pro Pro Tyr Gly His Val Asp Ile His Glu Asp Pro 
                165                 170                 175     


Gly Pro Pro Pro Pro Ser Pro Leu Val Gly Leu Lys Pro Leu Gln Leu 
            180                 185                 190         


Leu Glu Ile Lys Ala Arg Gly Arg Phe Gly Cys Val Trp Lys Ala Gln 
        195                 200                 205             


Leu Met Asn Asp Phe Val Ala Val Lys Ile Phe Pro Leu Gln Asp Lys 
    210                 215                 220                 


Gln Ser Trp Gln Ser Glu Arg Glu Ile Phe Ser Thr Pro Gly Met Lys 
225                 230                 235                 240 


His Glu Asn Leu Leu Gln Phe Ile Ala Ala Glu Lys Arg Gly Ser Asn 
                245                 250                 255     


Leu Glu Val Glu Leu Trp Leu Ile Thr Ala Phe His Asp Lys Gly Ser 
            260                 265                 270         


Leu Thr Asp Tyr Leu Lys Gly Asn Ile Ile Thr Trp Asn Glu Leu Cys 
        275                 280                 285             


His Val Ala Glu Thr Met Ser Arg Gly Leu Ser Tyr Leu His Glu Asp 
    290                 295                 300                 


Val Pro Trp Cys Arg Gly Glu Gly His Lys Pro Ser Ile Ala His Arg 
305                 310                 315                 320 


Asp Phe Lys Ser Lys Asn Val Leu Leu Lys Ser Asp Leu Thr Ala Val 
                325                 330                 335     


Leu Ala Asp Phe Gly Leu Ala Val Arg Phe Glu Pro Gly Lys Pro Pro 
            340                 345                 350         


Gly Asp Thr His Gly Gln Val Gly Thr Arg Arg Tyr Met Ala Pro Glu 
        355                 360                 365             


Val Leu Glu Gly Ala Ile Asn Phe Gln Arg Asp Ala Phe Leu Arg Ile 
    370                 375                 380                 


Asp Met Tyr Ala Met Gly Leu Val Leu Trp Glu Leu Val Ser Arg Cys 
385                 390                 395                 400 


Lys Ala Ala Asp Gly Pro Val Asp Glu Tyr Met Leu Pro Phe Glu Glu 
                405                 410                 415     


Glu Ile Gly Gln His Pro Ser Leu Glu Glu Leu Gln Glu Val Val Val 
            420                 425                 430         


His Lys Lys Met Arg Pro Thr Ile Lys Asp His Trp Leu Lys His Pro 
        435                 440                 445             


Gly Leu Ala Gln Leu Cys Val Thr Ile Glu Glu Cys Trp Asp His Asp 
    450                 455                 460                 


Ala Glu Ala Arg Leu Ser Ala Gly Cys Val Glu Glu Arg Val Ser Leu 
465                 470                 475                 480 


Ile Arg Arg Ser Val Asn Gly Thr Thr Ser Asp Cys Leu Val Ser Leu 
                485                 490                 495     


Val Thr Ser Val Thr Asn Val Asp Leu Pro Pro Lys Glu Ser Ser Ile 
            500                 505                 510         


<210>  29
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 19-134 of SEQ ID NO:28)

<400>  29

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105                 110         


Thr Ala Pro Thr 
        115     


<210>  30
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the C-terminal 15 amino acids deleted (amino acids 
       19-119 of SEQ ID NO:28)

<400>  30

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala 
            100     


<210>  31
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:28)

<400>  31

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr 
        115 


<210>  32
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the C-terminal 15 amino acids deleted (amino acids 
       20-119 of SEQ ID NO:28)

<400>  32

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala 
            100 


<210>  33
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence with the N-terminal 6 amino acids of the EC domain 
       deleted and the C-terminal 3 amino acids of the EC domain deleted
       (amino acids 25-131 of SEQ ID NO:16) and with an L79D mutation

<400>  33

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105         


<210>  34
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unprocessed ActRIIB-Fc fusion protein with the N-terminal 6 amino
       acids of the EC domain deleted and the C-terminal 3 amino acids 
       of the EC domain deleted (amino acids 25-131 of SEQ ID NO:16) and
       with an L79D mutation and with TPA leader sequence

<400>  34

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Gly Ala Ala Glu Thr Arg Glu Cys Ile Tyr 
            20                  25                  30          


Tyr Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu 
        35                  40                  45              


Arg Cys Glu Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp 
    50                  55                  60                  


Ala Asn Ser Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp 
65                  70                  75                  80  


Asp Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu 
                85                  90                  95      


Asn Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu 
            100                 105                 110         


Arg Phe Thr His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu 
        115                 120                 125             


Pro Pro Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
    130                 135                 140                 


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
145                 150                 155                 160 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
                165                 170                 175     


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
            180                 185                 190         


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
        195                 200                 205             


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
    210                 215                 220                 


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
225                 230                 235                 240 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
                245                 250                 255     


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
            260                 265                 270         


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
        275                 280                 285             


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
    290                 295                 300                 


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
305                 310                 315                 320 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
                325                 330                 335     


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
            340                 345                 350         


Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360 


<210>  35
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Processed ActRIIB-Fc fusion protein with the N-terminal 6 amino 
       acids of the EC domain deleted and the C-terminal 3 amino acids 
       of the EC domain deleted (amino acids 25-131 of SEQ ID NO:16) and
       with an L79D mutation

<400>  35

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr Gly Gly Gly Thr His 
            100                 105                 110         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        115                 120                 125             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    130                 135                 140                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
145                 150                 155                 160 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                165                 170                 175     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            180                 185                 190         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        195                 200                 205             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    210                 215                 220                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
225                 230                 235                 240 


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                245                 250                 255     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            260                 265                 270         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        275                 280                 285             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    290                 295                 300                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
305                 310                 315                 320 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330                 335 


<210>  36
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:28) with L79D mutation

<400>  36

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr 
        115 


<210>  37
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:16) with L79D mutation

<400>  37

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr 
        115 


<210>  38
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:28) with L79D mutation 
       fused to an Fc domain with a GGG linker

<400>  38

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
        115                 120                 125             


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
    130                 135                 140                 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
145                 150                 155                 160 


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
                165                 170                 175     


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
            180                 185                 190         


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
        195                 200                 205             


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
    210                 215                 220                 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
225                 230                 235                 240 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
                245                 250                 255     


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
            260                 265                 270         


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
        275                 280                 285             


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
    290                 295                 300                 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
305                 310                 315                 320 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
                325                 330                 335     


Leu Ser Leu Ser Pro Gly Lys 
            340             


<210>  39
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:16) with L79D mutation 
       fused to an Fc domain

<400>  39

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105                 110         


Ala Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
        115                 120                 125             


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
    130                 135                 140                 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
145                 150                 155                 160 


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
                165                 170                 175     


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
            180                 185                 190         


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
        195                 200                 205             


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
    210                 215                 220                 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
225                 230                 235                 240 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
                245                 250                 255     


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
            260                 265                 270         


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
        275                 280                 285             


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
    290                 295                 300                 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
305                 310                 315                 320 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
                325                 330                 335     


Leu Ser Leu Ser Pro Gly Lys 
            340             


<210>  40
<211>  368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:28) with L79D mutation 
       fused to an Fc domain and with TPA leader sequence

<400>  40

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Gly Ala Ser Gly Arg Gly Glu Ala Glu Thr 
            20                  25                  30          


Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn 
        35                  40                  45              


Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg Leu His 
    50                  55                  60                  


Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu Val Lys 
65                  70                  75                  80  


Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys 
                85                  90                  95      


Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly 
            100                 105                 110         


Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly Gly Pro 
        115                 120                 125             


Glu Val Thr Tyr Glu Pro Pro Pro Thr Ala Pro Thr Gly Gly Gly Thr 
    130                 135                 140                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
145                 150                 155                 160 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                165                 170                 175     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            180                 185                 190         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        195                 200                 205             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    210                 215                 220                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
225                 230                 235                 240 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                245                 250                 255     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            260                 265                 270         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        275                 280                 285             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    290                 295                 300                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
305                 310                 315                 320 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                325                 330                 335     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            340                 345                 350         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 365             


<210>  41
<211>  368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence (amino acids 20-134 of SEQ ID NO:16) with L79D mutation 
       fused to an Fc domain and with TPA leader sequence

<400>  41

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Gly Ala Ser Gly Arg Gly Glu Ala Glu Thr 
            20                  25                  30          


Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg Thr Asn 
        35                  40                  45              


Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg Leu His 
    50                  55                  60                  


Cys Tyr Ala Ser Trp Ala Asn Ser Ser Gly Thr Ile Glu Leu Val Lys 
65                  70                  75                  80  


Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln Glu Cys 
                85                  90                  95      


Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys Glu Gly 
            100                 105                 110         


Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly Gly Pro 
        115                 120                 125             


Glu Val Thr Tyr Glu Pro Pro Pro Thr Ala Pro Thr Gly Gly Gly Thr 
    130                 135                 140                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
145                 150                 155                 160 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                165                 170                 175     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            180                 185                 190         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        195                 200                 205             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    210                 215                 220                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
225                 230                 235                 240 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                245                 250                 255     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            260                 265                 270         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        275                 280                 285             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    290                 295                 300                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
305                 310                 315                 320 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                325                 330                 335     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            340                 345                 350         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 365             


<210>  42
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence having a variant C-terminal sequence (disclosed in 
       WO2007/053775)

<400>  42

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Gly Pro Trp Ala Ser Thr Thr Ile 
            100                 105                 110         


Pro Ser Gly Gly Pro Glu Ala Thr Ala Ala Ala Gly Asp Gln Gly Ser 
        115                 120                 125             


Gly Ala Leu Trp Leu Cys Leu Glu Gly Pro Ala His Glu 
    130                 135                 140     


<210>  43
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence having a variant C-terminal sequence (disclosed in 
       WO2007/053775) having an L79D mutation

<400>  43

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Gly Pro Trp Ala Ser Thr Thr Ile 
            100                 105                 110         


Pro Ser Gly Gly Pro Glu Ala Thr Ala Ala Ala Gly Asp Gln Gly Ser 
        115                 120                 125             


Gly Ala Leu Trp Leu Cys Leu Glu Gly Pro Ala His Glu 
    130                 135                 140     


<210>  44
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human ActRIIB soluble (extracellular), processed polypeptide 
       sequence having a variant C-terminal sequence (disclosed in 
       WO2007/053775) having an L79D mutation fused to an Fc domain with
       a TGGG linker

<400>  44

Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn 
1               5                   10                  15      


Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly 
            20                  25                  30          


Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser 
        35                  40                  45              


Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His 
                85                  90                  95      


Leu Pro Glu Ala Gly Gly Pro Glu Gly Pro Trp Ala Ser Thr Thr Ile 
            100                 105                 110         


Pro Ser Gly Gly Pro Glu Ala Thr Ala Ala Ala Gly Asp Gln Gly Ser 
        115                 120                 125             


Gly Ala Leu Trp Leu Cys Leu Glu Gly Pro Ala His Glu Thr Gly Gly 
    130                 135                 140                 


Gly Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
145                 150                 155                 160 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                165                 170                 175     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            180                 185                 190         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        195                 200                 205             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    210                 215                 220                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
225                 230                 235                 240 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                245                 250                 255     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            260                 265                 270         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        275                 280                 285             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    290                 295                 300                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
305                 310                 315                 320 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                325                 330                 335     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            340                 345                 350         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        355                 360                 365             


Gly Lys 
    370 


<210>  45
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding SEQ ID NO:24

<400>  45
atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt       60

tcgcccggcg ccgccgaaac ccgcgaatgt atttattaca atgctaattg ggaactcgaa      120

cggacgaacc aatccgggct cgaacggtgt gagggggaac aggataaacg cctccattgc      180

tatgcgtcgt ggaggaactc ctccgggacg attgaactgg tcaagaaagg gtgctgggac      240

gacgatttca attgttatga ccgccaggaa tgtgtcgcga ccgaagagaa tccgcaggtc      300

tatttctgtt gttgcgaggg gaatttctgt aatgaacggt ttacccacct ccccgaagcc      360

ggcgggcccg aggtgaccta tgaacccccg cccaccggtg gtggaactca cacatgccca      420

ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc      480

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc      540

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc      600

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc      660

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc      720

ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag      780

gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc      840

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg      900

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctat      960

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg     1020

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc cccgggtaaa     1080

tga                                                                   1083


<210>  46
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising a soluble extracellular domain of 
       ActRIIB (R64; SEQ ID NO:29) fused to an Fc domain

<400>  46

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105                 110         


Thr Ala Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
        115                 120                 125             


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    130                 135                 140                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
145                 150                 155                 160 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                165                 170                 175     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            180                 185                 190         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        195                 200                 205             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    210                 215                 220                 


Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
225                 230                 235                 240 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
                245                 250                 255     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            260                 265                 270         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        275                 280                 285             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    290                 295                 300                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
305                 310                 315                 320 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                325                 330                 335     


Ser Leu Ser Leu Ser Pro Gly Lys 
            340                 


<210>  47
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein comprising a soluble extracellular domain of 
       ActRIIB (R64) with the C-terminal 15 amino acids deleted (SEQ ID 
       NO:30) fused to an Fc domain

<400>  47

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Arg Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro 
            100                 105                 110         


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
        115                 120                 125             


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
    130                 135                 140                 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
145                 150                 155                 160 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
                165                 170                 175     


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
            180                 185                 190         


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
        195                 200                 205             


Ala Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
    210                 215                 220                 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
225                 230                 235                 240 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
                245                 250                 255     


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
            260                 265                 270         


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
    290                 295                 300                 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Runx2 qRT-PCR primer 1

<400>  48
ccgcacgaca accgcaccat                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Runx2 qRT-PCR primer 2

<400>  49
cgctccggcc cacaaatctc                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alp qRT-PCR primer 1

<400>  50
acgtggctaa gaatgtcatc                                                   20


<210>  51
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alp qRT-PCR primer 2

<400>  51
ctggtaggcg atgtcctta                                                    19


<210>  52
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  osterix qRT-PCR primer 1

<400>  52
tagtggtttg gggtttgttt accgc                                             25


<210>  53
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  osterix qRT-PCR primer 2

<400>  53
aaccaaataa ctcttattcc ctaagt                                            26


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Klotho qRT-PCR primer 1

<400>  54
gctctcaaag cccacatact g                                                 21


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Klotho qRT-PCR primer 1

<400>  55
gcagcataac gatagaggcc                                                   20


<210>  56
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sm22-alpha qRT-PCR primer 1

<400>  56
gttccagact gttgacctct tt                                                22


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sm22-alpha qRT-PCR primer 2

<400>  57
ctgcgctttc ttcataaacc                                                   20


<210>  58
<211>  5553
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Runx2 mRNA

<400>  58
gtgtgaatgc ttcattcgcc tcacaaacaa ccacagaacc acaagtgcgg tgcaaacttt       60

ctccaggagg acagcaagaa gtctctggtt tttaaatggt taatctccgc aggtcactac      120

cagccaccga gaccaacaga gtcatttaag gctgcaagca gtatttacaa cagagggtac      180

aagttctatc tgaaaaaaaa aggagggact atggcatcaa acagcctctt cagcacagtg      240

acaccatgtc agcaaaactt cttttgggat ccgagcacca gccggcgctt cagccccccc      300

tccagcagcc tgcagcccgg caaaatgagc gacgtgagcc cggtggtggc tgcgcaacag      360

cagcagcaac agcagcagca gcaacagcag cagcagcagc agcaacagca gcagcagcag      420

caggaggcgg cggcggcggc tgcggcggcg gcggcggctg cggcggcggc agctgcagtg      480

ccccggttgc ggccgcccca cgacaaccgc accatggtgg agatcatcgc cgaccacccg      540

gccgaactcg tccgcaccga cagccccaac ttcctgtgct cggtgctgcc ctcgcactgg      600

cgctgcaaca agaccctgcc cgtggccttc aaggtggtag ccctcggaga ggtaccagat      660

gggactgtgg ttactgtcat ggcgggtaac gatgaaaatt attctgctga gctccggaat      720

gcctctgctg ttatgaaaaa ccaagtagca aggttcaacg atctgagatt tgtgggccgg      780

agtggacgag gcaagagttt caccttgacc ataaccgtct tcacaaatcc tccccaagta      840

gctacctatc acagagcaat taaagttaca gtagatggac ctcgggaacc cagaaggcac      900

agacagaagc ttgatgactc taaacctagt ttgttctctg accgcctcag tgatttaggg      960

cgcattcctc atcccagtat gagagtaggt gtcccgcctc agaacccacg gccctccctg     1020

aactctgcac caagtccttt taatccacaa ggacagagtc agattacaga ccccaggcag     1080

gcacagtctt ccccgccgtg gtcctatgac cagtcttacc cctcctacct gagccagatg     1140

acgtccccgt ccatccactc taccaccccg ctgtcttcca cacggggcac tgggcttcct     1200

gccatcaccg atgtgcctag gcgcatttca gatgatgaca ctgccacctc tgacttctgc     1260

ctctggcctt ccactctcag taagaagagc caggcaggtg cttcagaact gggccctttt     1320

tcagacccca ggcagttccc aagcatttca tccctcactg agagccgctt ctccaaccca     1380

cgaatgcact atccagccac ctttacttac accccgccag tcacctcagg catgtccctc     1440

ggtatgtccg ccaccactca ctaccacacc tacctgccac caccctaccc cggctcttcc     1500

caaagccaga gtggaccctt ccagaccagc agcactccat atctctacta tggcacttcg     1560

tcaggatcct atcagtttcc catggtgccg gggggagacc ggtctccttc cagaatgctt     1620

ccgccatgca ccaccacctc gaatggcagc acgctattaa atccaaattt gcctaaccag     1680

aatgatggtg ttgacgctga tggaagccac agcagttccc caactgtttt gaattctagt     1740

ggcagaatgg atgaatctgt ttggcgacca tattgaaatt cctcagcagt ggcccagtgg     1800

tatctggggg ccacatccca cacgtatcaa tatatacata tatagagaga gtgcatatat     1860

atgtatatcg attagctatc tacaaagtgc ctatttttta gaagattttt cattcactca     1920

ctcagtcatg atcttgcagc cataagaggg tagatattga gaagcagaag gctcaagaga     1980

gacaattgca atcgagcttc agattgttta ctatttaaga tgtactttta caaaggaaca     2040

aagaagggaa aaggtatttt tgtttttgtt gtttggtctg ttatcatcaa taacctgttc     2100

atatgccaat tcagagaggt ggactccagg ttcaggaggg agaagagcaa agccgcttcc     2160

tctctgtgct ttgaaacttc acaccctcac ggtggcagct gtgtatggac cagtgccctc     2220

cgcagacagc tcacaaaacc agttgaggtg cactaaaggg acatgaggta gaatggatgc     2280

ttccatcaca gtaccatcat tcagaataac tcttccaatt tctgctttca gacatgctgc     2340

aggtcctcat ctgaactgtt gggttcgttt tttttttttt ttttcctgct ccaagaaagt     2400

gacttcaaaa ataactgatc aggatagatt attttatttt actttttaac actccttctc     2460

cccttttccc actgaaccaa aaagaaatcc catccctaaa acctgccttc tccttttatg     2520

caaaactgaa aatggcaata cattattata gccataatgg tatagatagt gattgcgttt     2580

ggctatgtgt tgttttcttt ttttttaaat tatgaatatg tgtaaaatct gaggtaactt     2640

gctaacgtga atggtcatat aactttaaag atatatttat aattatttaa tgacatttgg     2700

acccttgaaa catttcttag tgtattgata tgttgacttc ggtctctaaa agtgctcttt     2760

attaaataac aaatttcttc agtggtctag agccatatct gaaatattgc taagcaattt     2820

cagttcatcc aggcacaatg tgattttaaa aaatacttcc atctccaaat attttagata     2880

tagattgttt ttgtgatgta tgaaggaaat gttatgttta gttctttcag atctttgaat     2940

gcctctaaca cagctttgcc ttctaaagcg gtaattaggg atttaaaaaa caacctttag     3000

ccctttatca gcatgaaatg ctggagtgat gtggttttct aatttctttg gggtaattat     3060

gactcttgtc atattaaaaa gacaagcaca agtaaatcat tgaactacag aaaaatgttc     3120

tgtggtttca tagttaagca aaactctaaa tcgccaggct tcatagcaaa gacatagtca     3180

gctaaaagcc gcacatgtgg atagagggtt caattatgag acacctagta caggagagca     3240

aaattgcacc agagattctt aaccaaccag ccttaccaaa caacacaaca ggggaacccc     3300

aatctgcctt acccaaggcc ccactggcag ctttccacag aatttgcatt tagaggagca     3360

gaatgacatc actgtccttt gggagtaggt cctctgaaaa ggcagcaggt tccagcaggt     3420

agctgagctg agaggacata tggcccacgg ggacctacag acagcctttg acatttgtat     3480

ttcttacaat ggagggccaa ggagggcaag gggctgtgga gtttggtgtc tactagtgtg     3540

tatgaatttg agctagagtc cttctgtggc atgcactttg accactcctg gcagtcacat     3600

ggcagatttc caagtgcaaa tccttaatcc aaacaaggat catctaatga caccaccagg     3660

ccaatccctg ctctcctccc cgaaaagtca gggtcccttc attggaatcc tccacccacc     3720

caagcagaat ttagcagaga tttgccttca aaccctaacg gcccccttgt tctctggtcc     3780

ttctcaaacc cacctttgta ggccacccag cattgcagga cagcgtgtgg ggcagctgga     3840

cctgtgcttc ctgcctggga gtctcccttg gaattcatcc tgactccttc taataaaaat     3900

ggatgggaaa gcaaaacact ttgccttcta aaggccgtat accaagtatg cttagataaa     3960

taagccactt ttctattact taagtaagaa ggaagtagta attgatacta tttattgttt     4020

gtgtgtggta gcttgaagca caccactgtc catttatttg taagtgtaaa atatgtgtgt     4080

ttgtttcagc agcacttaaa aaagccagtg tctggttaca catttcaatt ttaattaatt     4140

gacataaaaa tgctaccgcc agtgccagct gcatcctatt taattaaaaa ggtactatat     4200

ttgtacatta ttttttaatg ttaaaagggc ttttttaagt ttacagtaca cataccgagt     4260

gactttaggg atgcttttgt gttgaaatgt tactatagtg gctgcaggca gcaacccaga     4320

aacactttag aagctttttt tccttgggaa aaattcaagc acttcttccc tccaccctca     4380

ctccaaccac cccaatgggg gtaattcaca tttcttagaa caaattctgc ccttttttgg     4440

tctagggatt aaaattttgt ttttctttct ttcttttttt ttttttttca ctgaaccctt     4500

aatttgcact gggtcatgtg tttgatttgt gatttcaaga ccaaagcaaa gtcttactac     4560

tactgtggaa ccatgtacta gttcctggga attaaaatag cgtggttctc tttgtagcac     4620

aaacattgct ggaatttgca gtcttttcaa tgcagccaca tttttatcca tttcagttgt     4680

ctcacaaatt ttaacccata tcagagttcc agaacaggta ccacagcttt ggttttagat     4740

tagtggaata acattcagcc cagaactgag aaactcaaca gattaactat cgtttgctct     4800

ttagacggtc tcactgcctc tcacttgcca gagccctttc aaaatgagca gagaagtcca     4860

caccattagg gaccatctgt gataaattca gaagggagga gatgtgtgta cagctttaag     4920

gattccctca attccgagga aagggactgg cccagaatcc aggttaatac atggaaacac     4980

gaagcattag caaaagtaat aattatacct atggtatttg aaagaacaat aataaaagac     5040

acttcttcca aaccttgaat ttgttgtttt tagaaaacga atgcatttaa aaatattttc     5100

tatgtgagaa ttttttagat gtgtgtttac ttcatgttta caaataactg tttgcttttt     5160

aatgcagtac tttgaaatat atcagccaaa accataactt acaataattt cttaggtatt     5220

ctgaataaaa ttccatttct tttggatatg ctttaccatt cttaggtttc tgtggaacaa     5280

aaatatttgt agcattttgt gtaaatacaa gctttcattt ttattttttc caattgctat     5340

tgcccaagaa ttgctttcca tgcacatatt gtaaaaattc cgctttgtgc cacaggtcat     5400

gattgtggat gagtttactc ttaacttcaa agggactatt tgtattgtat gttgcaactg     5460

taaattgaat tatttggcat ttttctcatg attgtaatat taatttgaag tttgaattta     5520

attttcaata aaatggcttt tttggttttg tta                                  5553


<210>  59
<211>  3173
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Alp mRNA

<400>  59
cggcgggcgg cagcagccta ggcagcagca gtagcagaag cagcagccgc cgagcagcag       60

caaggactct ggagtcagag taggactgta ggaccggagc ctgagtggaa caggagtgga      120

gctggcctgg gagagagcgg atccctccca gcaccctcag gccacccgtt gcctgcactc      180

tccctgccag acctccagag aggagagact cgggacagcc agccccaggt tcccccagct      240

ctctccatct gcctggctcc ttgggacccg ttccccagcc tcaggatggc gtcctccctg      300

cttgaggagg aagttcacta tggctccagt cccctggcca tgctgacggc agcgtgcagc      360

aaatttggtg gctctagccc tctgcgggac tcaacaactc tgggcaaagc aggcacaaag      420

aagccgtact ctgtgggcag tgacctttca gcctccaaaa ccatggggga tgcttatcca      480

gcccccttta caagcactaa tgggctcctt tcacctgcag gcagtcctcc agcacccacc      540

tcaggctatg ctaatgatta ccctcccttt tcccactcat tccctgggcc cacaggcacc      600

caggaccctg ggctactagt gcccaagggg cacagctctt ctgactgtct gcccagtgtc      660

tacacctctc tggacatgac acacccctat ggctcctggt acaaggcagg catccatgca      720

ggcatttcac caggcccagg caacactcct actccatggt gggatatgca ccctggaggc      780

aactggctag gtggtgggca gggccagggt gatgggctgc aagggacact gcccacaggt      840

ccagctcagc ctccactgaa cccccagctg cccacctacc catctgactt tgctcccctt      900

aatccagccc cctacccagc tccccacctc ttgcaaccag ggccccagca tgtcttgccc      960

caagatgtct ataaacccaa ggcagtggga aatagtgggc agctagaagg gagtggtgga     1020

gccaaacccc cacggggtgc aagcactggg ggtagtggtg gatatggggg cagtggggca     1080

gggcgctcct cctgcgactg ccctaattgc caggagctag agcggctggg agcagcagcg     1140

gctgggctgc ggaagaagcc catccacagc tgccacatcc ctggctgcgg caaggtgtat     1200

ggcaaggctt cgcacctgaa ggcccacttg cgctggcaca caggcgagag gcccttcgtc     1260

tgcaactggc tcttctgcgg caagaggttc actcgttcgg atgagctgga gcgtcatgtg     1320

cgcactcaca cccgggagaa gaagttcacc tgcctgctct gctccaagcg ctttacccga     1380

agcgaccacc tgagcaaaca ccagcgcacc catggagaac caggcccggg tccccctccc     1440

agtggcccca aggagctggg ggagggccgc agcacggggg aagaggaggc cagtcagacg     1500

ccccgacctt ctgcctcgcc agcaacccca gagaaagccc ctggaggcag ccctgagcag     1560

agcaacttgc tggagatctg agccgggtgg aaggtctccc accccagggc tgccctgaca     1620

gtctctcttg gctctctaga ccactgcttg ccaatcactc tctttacccc atgcatgcca     1680

tccttcgggg ctctctccct ctgtctccct cctggccatt ctgggcttgg gtatctcctt     1740

gcatgcctcc tcagctcacc ttctctcttc accatgagac tggctttcca caaactctca     1800

tctcaggccc tccccttgtg cctgatacct gcactccggc ttcctagact ctggccctgc     1860

cacaccaaca cactttctat ttgggctccc aacactattt ctccatctca ctccttgaca     1920

tgtacccctt tctgcttctc aagcttattt cctgctgtcc ctcagcctcc aggcttcagt     1980

cttcccaact tcttacacca ttgctttcca ttctccagaa ctcttttttc ctttttacaa     2040

acacaatgat aatgataatt tattgccccc tggtggcctc ttcatcaggg gtattggggt     2100

tagtgacctg gccagagggt gccaagaggg gggcagacca gtggggatct gatcccaaag     2160

atggggtgac cccagggtca gggaggctgc ccccaggcct gtatatttaa cccctatgta     2220

ccaggagtaa tgaatagtaa taattctatt tatgtaagtt atgatgacgg gtcaggtaga     2280

gtgagctggg gagggaagtg gatccatttc tgctaaggaa attctagtca aatgcatctc     2340

tgtatagaca aaatgttagt ggagaagatc ttgttaatag aatgtctatc atcagaatct     2400

cagttgatag ggtttctctt gtaatgaagt ctctacaaat tgggttagct acatctctgc     2460

taaacagttg atggggtatc tcttgattag ggggatccct aatatcccca gccccagcca     2520

gaagctgtga aacctcaagt cctatggagg ggagaaggac tggaatgtac cccatctccc     2580

ttgactgcag agcaggttcc tccactgccc caccccttag acaccatgac cccatcaggt     2640

taatcccctg ttgccatggt tatggagagc ttgcagctgc catcttagat gtgctctttg     2700

gggaagccca tctaacagga ggacattggt ttgggggtgc acctcctgaa gaatgggtgg     2760

ggaaggcttt ctctaggatc agattcaaat aagtatgtat tgagtgccta ctctgtgcaa     2820

ggcactatgc tagatctggt gcctagaagc cctgagaaag aacttaaaga gctaggagga     2880

cagaggcccc caagctgatc tggtggtgca tccacgcacc cccaccctgg gactttggat     2940

gctcccatct ccacctccag tgacttttaa agccgcttcg tgcctttcct gtaacgttgg     3000

atcctccttt tctgtcccct gctgtctcaa ggccccaagt taaagggtta aagccgctgg     3060

agcttgggga gagaacattg tggaatggaa gggatcatgc cctttgtgga gtcttttttt     3120

tttaatttaa taaataaaag ttggatttga aaaaaaaaaa aaaaaaaaaa aaa            3173


<210>  60
<211>  3173
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Osterix mRNA

<400>  60
cggcgggcgg cagcagccta ggcagcagca gtagcagaag cagcagccgc cgagcagcag       60

caaggactct ggagtcagag taggactgta ggaccggagc ctgagtggaa caggagtgga      120

gctggcctgg gagagagcgg atccctccca gcaccctcag gccacccgtt gcctgcactc      180

tccctgccag acctccagag aggagagact cgggacagcc agccccaggt tcccccagct      240

ctctccatct gcctggctcc ttgggacccg ttccccagcc tcaggatggc gtcctccctg      300

cttgaggagg aagttcacta tggctccagt cccctggcca tgctgacggc agcgtgcagc      360

aaatttggtg gctctagccc tctgcgggac tcaacaactc tgggcaaagc aggcacaaag      420

aagccgtact ctgtgggcag tgacctttca gcctccaaaa ccatggggga tgcttatcca      480

gcccccttta caagcactaa tgggctcctt tcacctgcag gcagtcctcc agcacccacc      540

tcaggctatg ctaatgatta ccctcccttt tcccactcat tccctgggcc cacaggcacc      600

caggaccctg ggctactagt gcccaagggg cacagctctt ctgactgtct gcccagtgtc      660

tacacctctc tggacatgac acacccctat ggctcctggt acaaggcagg catccatgca      720

ggcatttcac caggcccagg caacactcct actccatggt gggatatgca ccctggaggc      780

aactggctag gtggtgggca gggccagggt gatgggctgc aagggacact gcccacaggt      840

ccagctcagc ctccactgaa cccccagctg cccacctacc catctgactt tgctcccctt      900

aatccagccc cctacccagc tccccacctc ttgcaaccag ggccccagca tgtcttgccc      960

caagatgtct ataaacccaa ggcagtggga aatagtgggc agctagaagg gagtggtgga     1020

gccaaacccc cacggggtgc aagcactggg ggtagtggtg gatatggggg cagtggggca     1080

gggcgctcct cctgcgactg ccctaattgc caggagctag agcggctggg agcagcagcg     1140

gctgggctgc ggaagaagcc catccacagc tgccacatcc ctggctgcgg caaggtgtat     1200

ggcaaggctt cgcacctgaa ggcccacttg cgctggcaca caggcgagag gcccttcgtc     1260

tgcaactggc tcttctgcgg caagaggttc actcgttcgg atgagctgga gcgtcatgtg     1320

cgcactcaca cccgggagaa gaagttcacc tgcctgctct gctccaagcg ctttacccga     1380

agcgaccacc tgagcaaaca ccagcgcacc catggagaac caggcccggg tccccctccc     1440

agtggcccca aggagctggg ggagggccgc agcacggggg aagaggaggc cagtcagacg     1500

ccccgacctt ctgcctcgcc agcaacccca gagaaagccc ctggaggcag ccctgagcag     1560

agcaacttgc tggagatctg agccgggtgg aaggtctccc accccagggc tgccctgaca     1620

gtctctcttg gctctctaga ccactgcttg ccaatcactc tctttacccc atgcatgcca     1680

tccttcgggg ctctctccct ctgtctccct cctggccatt ctgggcttgg gtatctcctt     1740

gcatgcctcc tcagctcacc ttctctcttc accatgagac tggctttcca caaactctca     1800

tctcaggccc tccccttgtg cctgatacct gcactccggc ttcctagact ctggccctgc     1860

cacaccaaca cactttctat ttgggctccc aacactattt ctccatctca ctccttgaca     1920

tgtacccctt tctgcttctc aagcttattt cctgctgtcc ctcagcctcc aggcttcagt     1980

cttcccaact tcttacacca ttgctttcca ttctccagaa ctcttttttc ctttttacaa     2040

acacaatgat aatgataatt tattgccccc tggtggcctc ttcatcaggg gtattggggt     2100

tagtgacctg gccagagggt gccaagaggg gggcagacca gtggggatct gatcccaaag     2160

atggggtgac cccagggtca gggaggctgc ccccaggcct gtatatttaa cccctatgta     2220

ccaggagtaa tgaatagtaa taattctatt tatgtaagtt atgatgacgg gtcaggtaga     2280

gtgagctggg gagggaagtg gatccatttc tgctaaggaa attctagtca aatgcatctc     2340

tgtatagaca aaatgttagt ggagaagatc ttgttaatag aatgtctatc atcagaatct     2400

cagttgatag ggtttctctt gtaatgaagt ctctacaaat tgggttagct acatctctgc     2460

taaacagttg atggggtatc tcttgattag ggggatccct aatatcccca gccccagcca     2520

gaagctgtga aacctcaagt cctatggagg ggagaaggac tggaatgtac cccatctccc     2580

ttgactgcag agcaggttcc tccactgccc caccccttag acaccatgac cccatcaggt     2640

taatcccctg ttgccatggt tatggagagc ttgcagctgc catcttagat gtgctctttg     2700

gggaagccca tctaacagga ggacattggt ttgggggtgc acctcctgaa gaatgggtgg     2760

ggaaggcttt ctctaggatc agattcaaat aagtatgtat tgagtgccta ctctgtgcaa     2820

ggcactatgc tagatctggt gcctagaagc cctgagaaag aacttaaaga gctaggagga     2880

cagaggcccc caagctgatc tggtggtgca tccacgcacc cccaccctgg gactttggat     2940

gctcccatct ccacctccag tgacttttaa agccgcttcg tgcctttcct gtaacgttgg     3000

atcctccttt tctgtcccct gctgtctcaa ggccccaagt taaagggtta aagccgctgg     3060

agcttgggga gagaacattg tggaatggaa gggatcatgc cctttgtgga gtcttttttt     3120

tttaatttaa taaataaaag ttggatttga aaaaaaaaaa aaaaaaaaaa aaa            3173


<210>  61
<211>  5003
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Klotho mRNA

<400>  61
cgcgcagcat gcccgccagc gccccgccgc gccgcccgcg gccgccgccg ccgtcgctgt       60

cgctgctgct ggtgctgctg ggcctgggcg gccgccgcct gcgtgcggag ccgggcgacg      120

gcgcgcagac ctgggcccgt gtctcgcggc ctcctgcccc cgaggccgcg ggcctcttcc      180

agggcacctt ccccgacggc ttcctctggg ccgtgggcag cgccgcctac cagaccgagg      240

gcggctggca gcagcacggc aagggtgcgt ccatctggga cacgttcacc caccaccccc      300

tggcaccccc gggagactcc cggaacgcca gtctgccgtt gggcgccccg tcgccgctgc      360

agcccgccac cggggacgta gccagcgaca gctacaacaa cgtcttccgc gacacggagg      420

cgctgcgcga gctcggggtc actcactacc gcttctccat ctcgtgggcg cgagtgctcc      480

ccaatggcag cgcgggcgtc cccaaccgcg aggggctgcg ctactaccgg cgcctgctgg      540

agcggctgcg ggagctgggc gtgcagcccg tggtcaccct gtaccactgg gacctgcccc      600

agcgcctgca ggacgcctac ggcggctggg ccaaccgcgc cctggccgac cacttcaggg      660

attacgcgga gctctgcttc cgccacttcg gcggtcaggt caagtactgg atcaccatcg      720

acaaccccta cgtggtggcc tggcacggct acgccaccgg gcgcctggcc cccggcatcc      780

ggggcagccc gcggctcggg tacctggtgg cgcacaacct cctcctggct catgccaaag      840

tctggcatct ctacaatact tctttccgtc ccactcaggg aggtcaggtg tccattgccc      900

taagctctca ctggatcaat cctcgaagaa tgaccgacca cagcatcaaa gaatgtcaaa      960

aatctctgga ctttgtacta ggttggtttg ccaaacccgt atttattgat ggtgactatc     1020

ccgagagcat gaagaataac ctttcatcta ttctgcctga ttttactgaa tctgagaaaa     1080

agttcatcaa aggaactgct gacttttttg ctctttgctt tggacccacc ttgagttttc     1140

aacttttgga ccctcacatg aagttccgcc aattggaatc tcccaacctg aggcaactgc     1200

tttcctggat tgaccttgaa tttaaccatc ctcaaatatt tattgtggaa aatggctggt     1260

ttgtctcagg gaccaccaag agagatgatg ccaaatatat gtattacctc aaaaagttca     1320

tcatggaaac cttaaaagcc atcaagctgg atggggtgga tgtcatcggg tataccgcat     1380

ggtccctcat ggatggtttc gagtggcaca gaggttacag catcaggcgt ggactcttct     1440

atgttgactt tctaagccag gacaagatgt tgttgccaaa gtcttcagcc ttgttctacc     1500

aaaagctgat agagaaaaat ggcttccctc ctttacctga aaatcagccc ctagaaggga     1560

catttccctg tgactttgct tggggagttg ttgacaacta cattcaagta gataccactc     1620

tgtctcagtt taccgacctg aatgtttacc tgtgggatgt ccaccacagt aaaaggctta     1680

ttaaagtgga tggggttgtg accaagaaga ggaaatccta ctgtgttgac tttgctgcca     1740

tccagcccca gatcgcttta ctccaggaaa tgcacgttac acattttcgc ttctccctgg     1800

actgggccct gattctccct ctgggtaacc agtcccaggt gaaccacacc atcctgcagt     1860

actatcgctg catggccagc gagcttgtcc gtgtcaacat caccccagtg gtggccctgt     1920

ggcagcctat ggccccgaac caaggactgc cgcgcctcct ggccaggcag ggcgcctggg     1980

agaaccccta cactgccctg gcctttgcag agtatgcccg actgtgcttt caagagctcg     2040

gccatcacgt caagctttgg ataacgatga atgagccgta tacaaggaat atgacataca     2100

gtgctggcca caaccttctg aaggcccatg ccctggcttg gcatgtgtac aatgaaaagt     2160

ttaggcatgc tcagaatggg aaaatatcca tagccttgca ggctgattgg atagaacctg     2220

cctgcccttt ctcccaaaag gacaaagagg tggccgagag agttttggaa tttgacattg     2280

gctggctggc tgagcccatt ttcggctctg gagattatcc atgggtgatg agggactggc     2340

tgaaccaaag aaacaatttt cttcttcctt atttcactga agatgaaaaa aagctaatcc     2400

agggtacctt tgactttttg gctttaagcc attataccac catccttgta gactcagaaa     2460

aagaagatcc aataaaatac aatgattacc tagaagtgca agaaatgacc gacatcacgt     2520

ggctcaactc ccccagtcag gtggcggtag tgccctgggg gttgcgcaaa gtgctgaact     2580

ggctgaagtt caagtacgga gacctcccca tgtacataat atccaacgga atcgatgacg     2640

ggctgcatgc tgaggacgac cagctgaggg tgtattatat gcagaattac ataaacgaag     2700

ctctcaaagc ccacatactg gatggtatca atctttgcgg atactttgct tattcgttta     2760

acgaccgcac agctccgagg tttggcctct atcgttatgc tgcagatcag tttgagccca     2820

aggcatccat gaaacattac aggaaaatta ttgacagcaa tggtttcccg ggcccagaaa     2880

ctctggaaag attttgtcca gaagaattca ccgtgtgtac tgagtgcagt ttttttcaca     2940

cccgaaagtc tttactggct ttcatagctt ttctattttt tgcttctatt atttctctct     3000

cccttatatt ttactactcg aagaaaggca gaagaagtta caaatagttc tgaacatttt     3060

tctattcatt cattttgaaa taattatgca gacacatcag ctgttaacca tttgcacctc     3120

taagtgttgt gaaactgtaa atttcataca tttgacttct agaaaacatt tttgtggctt     3180

atgacagagg ttttgaaatg ggcataggtg atcgtaaaat attgaataat gcgaatagtg     3240

cctgaatttg ttctcttttt gggtgattaa aaaactgaca ggcactataa tttctgtaac     3300

acactaacaa aagcatgaaa aataggaacc acaccaatgc aacatttgtg cagaaatttg     3360

aatgacaaga ttaggaatat tttcttctgc acccacttct aaatttaatg tttttctgga     3420

agtagtaatt gcaagagttc gaatagaaag ttatgtacca agtaaccatt tctcagctgc     3480

cataataatg cctagtggct tcccctctgt caaatctagt ttcctatgga aaagaagatg     3540

gcagatacag gagagacgac agagggtcct aggctggaat gttcctttcg aaagcaatgc     3600

ttctatcaaa tactagtatt aatttatgta tctggttaat gacatacttg gagagcaaat     3660

tatggaaatg tgtattttat atgatttttg aggtcctgtc taaaccctgt gtccctgagg     3720

gatctgtctc actggcatct tgttgagggc cttgcacata ggaaactttt gataagtatc     3780

tgcggaaaaa caaacatgaa tcctgtgata ttgggctctt caggaagcat aaagcaattg     3840

tgaaatacag tataccgcag tggctctagg tggaggaaag gaggaaaaag tgcttattat     3900

gtgcaacatt atgattaatc tgattataca ccatttttga gcagatcttg gaatgaatga     3960

catgaccttt ccctagagaa taaggatgaa ataatcactc attctatgaa cagtgacact     4020

actttctatt ctttagctgt actgtaattt ctttgagttg atagttttac aaattcttaa     4080

taggttcaaa agcaatctgg tctgaataac actggatttg tttctgtgat ctctgaggtc     4140

tattttatgt ttttgctgct acttctgtgg aagtagcttt gaactagttt tactttgaac     4200

tttcacgctg aaacatgcta gtgatatcta gaaagggcta attaggtctc atcctttaat     4260

gccccttaaa taagtcttgc tgattttcag acagggaagt ctctctatta cactggagct     4320

gttttataga taagtcaata ttgtatcagg caagataaac caatgtcata acaggcattg     4380

ccaacctcac tgacacaggg tcatagtgta taataatata ctgtactata taatatatca     4440

tctttagagg tatgattttt tcatgaaaga taagcttttg gtaatattca ttttaaagtg     4500

gacttattaa aattggatgc tagagaatca agtttatttt atgtatatat ttttctgatt     4560

ataagagtaa tatatgttca ttgtaaaaat ttttaaaaca cagaaactat atgcaaagaa     4620

aaaataaaaa ttatctataa tctcagaacc cagaaatagc cactattaac atttcctacg     4680

tattttattt tacatagatc atattgtata tagttagtat ctttattaat ttttattatg     4740

aaactttcct ttgtcattat tagtcttcaa aagcatgatt tttaatagtt gttgagtatt     4800

ccaccacagg aatgtatcac aacttaaccg ttcccgtttg ttagactagt ttcttattaa     4860

tgttgatgaa tgttgtttaa aaataatttt gttgctacat ttactttaat ttccttgact     4920

gtaaagagaa gtaattttgc tccttgataa agtattatat taataataaa tctgcctgca     4980

actttttgcc ttctttcata atc                                             5003


<210>  62
<211>  1574
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Sm22-alpha mRNA

<400>  62
tcaccacggc ggcagccctt taaacccctc acccagccag cgccccatcc tgtctgtccg       60

aacccagaca caagtcttca ctccttcctg cgagccctga ggaagccttg tgagtgcatt      120

ggctggggct tggagggaag ttgggctgga gctggacagg agcagtgggt gcatttcagg      180

caggctctcc tgaggtccca ggcgccagct ccagctccct ggctagggaa acccaccctc      240

tcagtcagca tgggggccca agctccaggc agggtgggct ggatcactag cgtcctggat      300

ctctctcaga ctgggcagcc ccgggctcat tgaaatgccc cggatgactt ggctagtgca      360

gaggaattga tggaaaccac cggggtgaga gggaggctcc ccatctcagc cagccacatc      420

cacaaggtgt gtgtaagggt gcaggcgccg gccggttagg ccaaggctct actgtctgtt      480

gcccctccag gagaacttcc aaggagcttt ccccagacat ggccaacaag ggtccttcct      540

atggcatgag ccgcgaagtg cagtccaaaa tcgagaagaa gtatgacgag gagctggagg      600

agcggctggt ggagtggatc atagtgcagt gtggccctga tgtgggccgc ccagaccgtg      660

ggcgcttggg cttccaggtc tggctgaaga atggcgtgat tctgagcaag ctggtgaaca      720

gcctgtaccc tgatggctcc aagccggtga aggtgcccga gaacccaccc tccatggtct      780

tcaagcagat ggagcaggtg gctcagttcc tgaaggcggc tgaggactat ggggtcatca      840

agactgacat gttccagact gttgacctct ttgaaggcaa agacatggca gcagtgcaga      900

ggaccctgat ggctttgggc agcttggcag tgaccaagaa tgatgggcac taccgtggag      960

atcccaactg gtttatgaag aaagcgcagg agcataagag ggaattcaca gagagccagc     1020

tgcaggaggg aaagcatgtc attggccttc agatgggcag caacagaggg gcctcccagg     1080

ccggcatgac aggctacgga cgacctcggc agatcatcag ttagagcgga gagggctagc     1140

cctgagcccg gccctccccc agctccttgg ctgcagccat cccgcttagc ctgcctcacc     1200

cacacccgtg tggtaccttc agccctggcc aagctttgag gctctgtcac tgagcaatgg     1260

taactgcacc tgggcagctc ctccctgtgc ccccagcctc agcccaactt cttacccgaa     1320

agcatcactg ccttggcccc tccctcccgg ctgcccccat cacctctact gtctcctccc     1380

tgggctaagc aggggagaag cgggctgggg gtagcctgga tgtgggccaa gtccactgtc     1440

ctccttggcg gcaaaagccc attgaagaag aaccagccca gcctgccccc tatcttgtcc     1500

tggaatattt ttggggttgg aactcaaaaa aaaaaaaaaa aaatcaatct tttctcaaaa     1560

aaaaaaaaaa aaaa                                                       1574


<210>  63
<211>  521
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Runx2 protein

<400>  63

Met Ala Ser Asn Ser Leu Phe Ser Thr Val Thr Pro Cys Gln Gln Asn 
1               5                   10                  15      


Phe Phe Trp Asp Pro Ser Thr Ser Arg Arg Phe Ser Pro Pro Ser Ser 
            20                  25                  30          


Ser Leu Gln Pro Gly Lys Met Ser Asp Val Ser Pro Val Val Ala Ala 
        35                  40                  45              


Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln 
    50                  55                  60                  


Gln Gln Gln Gln Gln Gln Gln Glu Ala Ala Ala Ala Ala Ala Ala Ala 
65                  70                  75                  80  


Ala Ala Ala Ala Ala Ala Ala Ala Ala Val Pro Arg Leu Arg Pro Pro 
                85                  90                  95      


His Asp Asn Arg Thr Met Val Glu Ile Ile Ala Asp His Pro Ala Glu 
            100                 105                 110         


Leu Val Arg Thr Asp Ser Pro Asn Phe Leu Cys Ser Val Leu Pro Ser 
        115                 120                 125             


His Trp Arg Cys Asn Lys Thr Leu Pro Val Ala Phe Lys Val Val Ala 
    130                 135                 140                 


Leu Gly Glu Val Pro Asp Gly Thr Val Val Thr Val Met Ala Gly Asn 
145                 150                 155                 160 


Asp Glu Asn Tyr Ser Ala Glu Leu Arg Asn Ala Ser Ala Val Met Lys 
                165                 170                 175     


Asn Gln Val Ala Arg Phe Asn Asp Leu Arg Phe Val Gly Arg Ser Gly 
            180                 185                 190         


Arg Gly Lys Ser Phe Thr Leu Thr Ile Thr Val Phe Thr Asn Pro Pro 
        195                 200                 205             


Gln Val Ala Thr Tyr His Arg Ala Ile Lys Val Thr Val Asp Gly Pro 
    210                 215                 220                 


Arg Glu Pro Arg Arg His Arg Gln Lys Leu Asp Asp Ser Lys Pro Ser 
225                 230                 235                 240 


Leu Phe Ser Asp Arg Leu Ser Asp Leu Gly Arg Ile Pro His Pro Ser 
                245                 250                 255     


Met Arg Val Gly Val Pro Pro Gln Asn Pro Arg Pro Ser Leu Asn Ser 
            260                 265                 270         


Ala Pro Ser Pro Phe Asn Pro Gln Gly Gln Ser Gln Ile Thr Asp Pro 
        275                 280                 285             


Arg Gln Ala Gln Ser Ser Pro Pro Trp Ser Tyr Asp Gln Ser Tyr Pro 
    290                 295                 300                 


Ser Tyr Leu Ser Gln Met Thr Ser Pro Ser Ile His Ser Thr Thr Pro 
305                 310                 315                 320 


Leu Ser Ser Thr Arg Gly Thr Gly Leu Pro Ala Ile Thr Asp Val Pro 
                325                 330                 335     


Arg Arg Ile Ser Asp Asp Asp Thr Ala Thr Ser Asp Phe Cys Leu Trp 
            340                 345                 350         


Pro Ser Thr Leu Ser Lys Lys Ser Gln Ala Gly Ala Ser Glu Leu Gly 
        355                 360                 365             


Pro Phe Ser Asp Pro Arg Gln Phe Pro Ser Ile Ser Ser Leu Thr Glu 
    370                 375                 380                 


Ser Arg Phe Ser Asn Pro Arg Met His Tyr Pro Ala Thr Phe Thr Tyr 
385                 390                 395                 400 


Thr Pro Pro Val Thr Ser Gly Met Ser Leu Gly Met Ser Ala Thr Thr 
                405                 410                 415     


His Tyr His Thr Tyr Leu Pro Pro Pro Tyr Pro Gly Ser Ser Gln Ser 
            420                 425                 430         


Gln Ser Gly Pro Phe Gln Thr Ser Ser Thr Pro Tyr Leu Tyr Tyr Gly 
        435                 440                 445             


Thr Ser Ser Gly Ser Tyr Gln Phe Pro Met Val Pro Gly Gly Asp Arg 
    450                 455                 460                 


Ser Pro Ser Arg Met Leu Pro Pro Cys Thr Thr Thr Ser Asn Gly Ser 
465                 470                 475                 480 


Thr Leu Leu Asn Pro Asn Leu Pro Asn Gln Asn Asp Gly Val Asp Ala 
                485                 490                 495     


Asp Gly Ser His Ser Ser Ser Pro Thr Val Leu Asn Ser Ser Gly Arg 
            500                 505                 510         


Met Asp Glu Ser Val Trp Arg Pro Tyr 
        515                 520     


<210>  64
<211>  524
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Alp protein

<400>  64

Met Ile Ser Pro Phe Leu Val Leu Ala Ile Gly Thr Cys Leu Thr Asn 
1               5                   10                  15      


Ser Leu Val Pro Glu Lys Glu Lys Asp Pro Lys Tyr Trp Arg Asp Gln 
            20                  25                  30          


Ala Gln Glu Thr Leu Lys Tyr Ala Leu Glu Leu Gln Lys Leu Asn Thr 
        35                  40                  45              


Asn Val Ala Lys Asn Val Ile Met Phe Leu Gly Asp Gly Met Gly Val 
    50                  55                  60                  


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Leu His His Asn 
65                  70                  75                  80  


Pro Gly Glu Glu Thr Arg Leu Glu Met Asp Lys Phe Pro Phe Val Ala 
                85                  90                  95      


Leu Ser Lys Thr Tyr Asn Thr Asn Ala Gln Val Pro Asp Ser Ala Gly 
            100                 105                 110         


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Ala Asn Glu Gly Thr Val 
        115                 120                 125             


Gly Val Ser Ala Ala Thr Glu Arg Ser Arg Cys Asn Thr Thr Gln Gly 
    130                 135                 140                 


Asn Glu Val Thr Ser Ile Leu Arg Trp Ala Lys Asp Ala Gly Lys Ser 
145                 150                 155                 160 


Val Gly Ile Val Thr Thr Thr Arg Val Asn His Ala Thr Pro Ser Ala 
                165                 170                 175     


Ala Tyr Ala His Ser Ala Asp Arg Asp Trp Tyr Ser Asp Asn Glu Met 
            180                 185                 190         


Pro Pro Glu Ala Leu Ser Gln Gly Cys Lys Asp Ile Ala Tyr Gln Leu 
        195                 200                 205             


Met His Asn Ile Arg Asp Ile Asp Val Ile Met Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Tyr Pro Lys Asn Lys Thr Asp Val Glu Tyr Glu Ser Asp Glu 
225                 230                 235                 240 


Lys Ala Arg Gly Thr Arg Leu Asp Gly Leu Asp Leu Val Asp Thr Trp 
                245                 250                 255     


Lys Ser Phe Lys Pro Arg Tyr Lys His Ser His Phe Ile Trp Asn Arg 
            260                 265                 270         


Thr Glu Leu Leu Thr Leu Asp Pro His Asn Val Asp Tyr Leu Leu Gly 
        275                 280                 285             


Leu Phe Glu Pro Gly Asp Met Gln Tyr Glu Leu Asn Arg Asn Asn Val 
    290                 295                 300                 


Thr Asp Pro Ser Leu Ser Glu Met Val Val Val Ala Ile Gln Ile Leu 
305                 310                 315                 320 


Arg Lys Asn Pro Lys Gly Phe Phe Leu Leu Val Glu Gly Gly Arg Ile 
                325                 330                 335     


Asp His Gly His His Glu Gly Lys Ala Lys Gln Ala Leu His Glu Ala 
            340                 345                 350         


Val Glu Met Asp Arg Ala Ile Gly Gln Ala Gly Ser Leu Thr Ser Ser 
        355                 360                 365             


Glu Asp Thr Leu Thr Val Val Thr Ala Asp His Ser His Val Phe Thr 
    370                 375                 380                 


Phe Gly Gly Tyr Thr Pro Arg Gly Asn Ser Ile Phe Gly Leu Ala Pro 
385                 390                 395                 400 


Met Leu Ser Asp Thr Asp Lys Lys Pro Phe Thr Ala Ile Leu Tyr Gly 
                405                 410                 415     


Asn Gly Pro Gly Tyr Lys Val Val Gly Gly Glu Arg Glu Asn Val Ser 
            420                 425                 430         


Met Val Asp Tyr Ala His Asn Asn Tyr Gln Ala Gln Ser Ala Val Pro 
        435                 440                 445             


Leu Arg His Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ser Lys 
    450                 455                 460                 


Gly Pro Met Ala His Leu Leu His Gly Val His Glu Gln Asn Tyr Val 
465                 470                 475                 480 


Pro His Val Met Ala Tyr Ala Ala Cys Ile Gly Ala Asn Leu Gly His 
                485                 490                 495     


Cys Ala Pro Ala Ser Ser Ala Gly Ser Leu Ala Ala Gly Pro Leu Leu 
            500                 505                 510         


Leu Ala Leu Ala Leu Tyr Pro Leu Ser Val Leu Phe 
        515                 520                 


<210>  65
<211>  431
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Osterix protein

<400>  65

Met Ala Ser Ser Leu Leu Glu Glu Glu Val His Tyr Gly Ser Ser Pro 
1               5                   10                  15      


Leu Ala Met Leu Thr Ala Ala Cys Ser Lys Phe Gly Gly Ser Ser Pro 
            20                  25                  30          


Leu Arg Asp Ser Thr Thr Leu Gly Lys Ala Gly Thr Lys Lys Pro Tyr 
        35                  40                  45              


Ser Val Gly Ser Asp Leu Ser Ala Ser Lys Thr Met Gly Asp Ala Tyr 
    50                  55                  60                  


Pro Ala Pro Phe Thr Ser Thr Asn Gly Leu Leu Ser Pro Ala Gly Ser 
65                  70                  75                  80  


Pro Pro Ala Pro Thr Ser Gly Tyr Ala Asn Asp Tyr Pro Pro Phe Ser 
                85                  90                  95      


His Ser Phe Pro Gly Pro Thr Gly Thr Gln Asp Pro Gly Leu Leu Val 
            100                 105                 110         


Pro Lys Gly His Ser Ser Ser Asp Cys Leu Pro Ser Val Tyr Thr Ser 
        115                 120                 125             


Leu Asp Met Thr His Pro Tyr Gly Ser Trp Tyr Lys Ala Gly Ile His 
    130                 135                 140                 


Ala Gly Ile Ser Pro Gly Pro Gly Asn Thr Pro Thr Pro Trp Trp Asp 
145                 150                 155                 160 


Met His Pro Gly Gly Asn Trp Leu Gly Gly Gly Gln Gly Gln Gly Asp 
                165                 170                 175     


Gly Leu Gln Gly Thr Leu Pro Thr Gly Pro Ala Gln Pro Pro Leu Asn 
            180                 185                 190         


Pro Gln Leu Pro Thr Tyr Pro Ser Asp Phe Ala Pro Leu Asn Pro Ala 
        195                 200                 205             


Pro Tyr Pro Ala Pro His Leu Leu Gln Pro Gly Pro Gln His Val Leu 
    210                 215                 220                 


Pro Gln Asp Val Tyr Lys Pro Lys Ala Val Gly Asn Ser Gly Gln Leu 
225                 230                 235                 240 


Glu Gly Ser Gly Gly Ala Lys Pro Pro Arg Gly Ala Ser Thr Gly Gly 
                245                 250                 255     


Ser Gly Gly Tyr Gly Gly Ser Gly Ala Gly Arg Ser Ser Cys Asp Cys 
            260                 265                 270         


Pro Asn Cys Gln Glu Leu Glu Arg Leu Gly Ala Ala Ala Ala Gly Leu 
        275                 280                 285             


Arg Lys Lys Pro Ile His Ser Cys His Ile Pro Gly Cys Gly Lys Val 
    290                 295                 300                 


Tyr Gly Lys Ala Ser His Leu Lys Ala His Leu Arg Trp His Thr Gly 
305                 310                 315                 320 


Glu Arg Pro Phe Val Cys Asn Trp Leu Phe Cys Gly Lys Arg Phe Thr 
                325                 330                 335     


Arg Ser Asp Glu Leu Glu Arg His Val Arg Thr His Thr Arg Glu Lys 
            340                 345                 350         


Lys Phe Thr Cys Leu Leu Cys Ser Lys Arg Phe Thr Arg Ser Asp His 
        355                 360                 365             


Leu Ser Lys His Gln Arg Thr His Gly Glu Pro Gly Pro Gly Pro Pro 
    370                 375                 380                 


Pro Ser Gly Pro Lys Glu Leu Gly Glu Gly Arg Ser Thr Gly Glu Glu 
385                 390                 395                 400 


Glu Ala Ser Gln Thr Pro Arg Pro Ser Ala Ser Pro Ala Thr Pro Glu 
                405                 410                 415     


Lys Ala Pro Gly Gly Ser Pro Glu Gln Ser Asn Leu Leu Glu Ile 
            420                 425                 430     


<210>  66
<211>  1012
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Klotho protein

<400>  66

Met Pro Ala Ser Ala Pro Pro Arg Arg Pro Arg Pro Pro Pro Pro Ser 
1               5                   10                  15      


Leu Ser Leu Leu Leu Val Leu Leu Gly Leu Gly Gly Arg Arg Leu Arg 
            20                  25                  30          


Ala Glu Pro Gly Asp Gly Ala Gln Thr Trp Ala Arg Phe Ser Arg Pro 
        35                  40                  45              


Pro Ala Pro Glu Ala Ala Gly Leu Phe Gln Gly Thr Phe Pro Asp Gly 
    50                  55                  60                  


Phe Leu Trp Ala Val Gly Ser Ala Ala Tyr Gln Thr Glu Gly Gly Trp 
65                  70                  75                  80  


Gln Gln His Gly Lys Gly Ala Ser Ile Trp Asp Thr Phe Thr His His 
                85                  90                  95      


Pro Leu Ala Pro Pro Gly Asp Ser Arg Asn Ala Ser Leu Pro Leu Gly 
            100                 105                 110         


Ala Pro Ser Pro Leu Gln Pro Ala Thr Gly Asp Val Ala Ser Asp Ser 
        115                 120                 125             


Tyr Asn Asn Val Phe Arg Asp Thr Glu Ala Leu Arg Glu Leu Gly Val 
    130                 135                 140                 


Thr His Tyr Arg Phe Ser Ile Ser Trp Ala Arg Val Leu Pro Asn Gly 
145                 150                 155                 160 


Ser Ala Gly Val Pro Asn Arg Glu Gly Leu Arg Tyr Tyr Arg Arg Leu 
                165                 170                 175     


Leu Glu Arg Leu Arg Glu Leu Gly Val Gln Pro Val Val Thr Leu Tyr 
            180                 185                 190         


His Trp Asp Leu Pro Gln Arg Leu Gln Asp Ala Tyr Gly Gly Trp Ala 
        195                 200                 205             


Asn Arg Ala Leu Ala Asp His Phe Arg Asp Tyr Ala Glu Leu Cys Phe 
    210                 215                 220                 


Arg His Phe Gly Gly Gln Val Lys Tyr Trp Ile Thr Ile Asp Asn Pro 
225                 230                 235                 240 


Tyr Val Val Ala Trp His Gly Tyr Ala Thr Gly Arg Leu Ala Pro Gly 
                245                 250                 255     


Ile Arg Gly Ser Pro Arg Leu Gly Tyr Leu Val Ala His Asn Leu Leu 
            260                 265                 270         


Leu Ala His Ala Lys Val Trp His Leu Tyr Asn Thr Ser Phe Arg Pro 
        275                 280                 285             


Thr Gln Gly Gly Gln Val Ser Ile Ala Leu Ser Ser His Trp Ile Asn 
    290                 295                 300                 


Pro Arg Arg Met Thr Asp His Ser Ile Lys Glu Cys Gln Lys Ser Leu 
305                 310                 315                 320 


Asp Phe Val Leu Gly Trp Phe Ala Lys Pro Val Phe Ile Asp Gly Asp 
                325                 330                 335     


Tyr Pro Glu Ser Met Lys Asn Asn Leu Ser Ser Ile Leu Pro Asp Phe 
            340                 345                 350         


Thr Glu Ser Glu Lys Lys Phe Ile Lys Gly Thr Ala Asp Phe Phe Ala 
        355                 360                 365             


Leu Cys Phe Gly Pro Thr Leu Ser Phe Gln Leu Leu Asp Pro His Met 
    370                 375                 380                 


Lys Phe Arg Gln Leu Glu Ser Pro Asn Leu Arg Gln Leu Leu Ser Trp 
385                 390                 395                 400 


Ile Asp Leu Glu Phe Asn His Pro Gln Ile Phe Ile Val Glu Asn Gly 
                405                 410                 415     


Trp Phe Val Ser Gly Thr Thr Lys Arg Asp Asp Ala Lys Tyr Met Tyr 
            420                 425                 430         


Tyr Leu Lys Lys Phe Ile Met Glu Thr Leu Lys Ala Ile Lys Leu Asp 
        435                 440                 445             


Gly Val Asp Val Ile Gly Tyr Thr Ala Trp Ser Leu Met Asp Gly Phe 
    450                 455                 460                 


Glu Trp His Arg Gly Tyr Ser Ile Arg Arg Gly Leu Phe Tyr Val Asp 
465                 470                 475                 480 


Phe Leu Ser Gln Asp Lys Met Leu Leu Pro Lys Ser Ser Ala Leu Phe 
                485                 490                 495     


Tyr Gln Lys Leu Ile Glu Lys Asn Gly Phe Pro Pro Leu Pro Glu Asn 
            500                 505                 510         


Gln Pro Leu Glu Gly Thr Phe Pro Cys Asp Phe Ala Trp Gly Val Val 
        515                 520                 525             


Asp Asn Tyr Ile Gln Val Asp Thr Thr Leu Ser Gln Phe Thr Asp Leu 
    530                 535                 540                 


Asn Val Tyr Leu Trp Asp Val His His Ser Lys Arg Leu Ile Lys Val 
545                 550                 555                 560 


Asp Gly Val Val Thr Lys Lys Arg Lys Ser Tyr Cys Val Asp Phe Ala 
                565                 570                 575     


Ala Ile Gln Pro Gln Ile Ala Leu Leu Gln Glu Met His Val Thr His 
            580                 585                 590         


Phe Arg Phe Ser Leu Asp Trp Ala Leu Ile Leu Pro Leu Gly Asn Gln 
        595                 600                 605             


Ser Gln Val Asn His Thr Ile Leu Gln Tyr Tyr Arg Cys Met Ala Ser 
    610                 615                 620                 


Glu Leu Val Arg Val Asn Ile Thr Pro Val Val Ala Leu Trp Gln Pro 
625                 630                 635                 640 


Met Ala Pro Asn Gln Gly Leu Pro Arg Leu Leu Ala Arg Gln Gly Ala 
                645                 650                 655     


Trp Glu Asn Pro Tyr Thr Ala Leu Ala Phe Ala Glu Tyr Ala Arg Leu 
            660                 665                 670         


Cys Phe Gln Glu Leu Gly His His Val Lys Leu Trp Ile Thr Met Asn 
        675                 680                 685             


Glu Pro Tyr Thr Arg Asn Met Thr Tyr Ser Ala Gly His Asn Leu Leu 
    690                 695                 700                 


Lys Ala His Ala Leu Ala Trp His Val Tyr Asn Glu Lys Phe Arg His 
705                 710                 715                 720 


Ala Gln Asn Gly Lys Ile Ser Ile Ala Leu Gln Ala Asp Trp Ile Glu 
                725                 730                 735     


Pro Ala Cys Pro Phe Ser Gln Lys Asp Lys Glu Val Ala Glu Arg Val 
            740                 745                 750         


Leu Glu Phe Asp Ile Gly Trp Leu Ala Glu Pro Ile Phe Gly Ser Gly 
        755                 760                 765             


Asp Tyr Pro Trp Val Met Arg Asp Trp Leu Asn Gln Arg Asn Asn Phe 
    770                 775                 780                 


Leu Leu Pro Tyr Phe Thr Glu Asp Glu Lys Lys Leu Ile Gln Gly Thr 
785                 790                 795                 800 


Phe Asp Phe Leu Ala Leu Ser His Tyr Thr Thr Ile Leu Val Asp Ser 
                805                 810                 815     


Glu Lys Glu Asp Pro Ile Lys Tyr Asn Asp Tyr Leu Glu Val Gln Glu 
            820                 825                 830         


Met Thr Asp Ile Thr Trp Leu Asn Ser Pro Ser Gln Val Ala Val Val 
        835                 840                 845             


Pro Trp Gly Leu Arg Lys Val Leu Asn Trp Leu Lys Phe Lys Tyr Gly 
    850                 855                 860                 


Asp Leu Pro Met Tyr Ile Ile Ser Asn Gly Ile Asp Asp Gly Leu His 
865                 870                 875                 880 


Ala Glu Asp Asp Gln Leu Arg Val Tyr Tyr Met Gln Asn Tyr Ile Asn 
                885                 890                 895     


Glu Ala Leu Lys Ala His Ile Leu Asp Gly Ile Asn Leu Cys Gly Tyr 
            900                 905                 910         


Phe Ala Tyr Ser Phe Asn Asp Arg Thr Ala Pro Arg Phe Gly Leu Tyr 
        915                 920                 925             


Arg Tyr Ala Ala Asp Gln Phe Glu Pro Lys Ala Ser Met Lys His Tyr 
    930                 935                 940                 


Arg Lys Ile Ile Asp Ser Asn Gly Phe Pro Gly Pro Glu Thr Leu Glu 
945                 950                 955                 960 


Arg Phe Cys Pro Glu Glu Phe Thr Val Cys Thr Glu Cys Ser Phe Phe 
                965                 970                 975     


His Thr Arg Lys Ser Leu Leu Ala Phe Ile Ala Phe Leu Phe Phe Ala 
            980                 985                 990         


Ser Ile Ile Ser Leu Ser Leu Ile  Phe Tyr Tyr Ser Lys  Lys Gly Arg 
        995                 1000                 1005             


Arg Ser  Tyr Lys 
    1010         


<210>  67
<211>  201
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Sm22-alpha protein

<400>  67

Met Ala Asn Lys Gly Pro Ser Tyr Gly Met Ser Arg Glu Val Gln Ser 
1               5                   10                  15      


Lys Ile Glu Lys Lys Tyr Asp Glu Glu Leu Glu Glu Arg Leu Val Glu 
            20                  25                  30          


Trp Ile Ile Val Gln Cys Gly Pro Asp Val Gly Arg Pro Asp Arg Gly 
        35                  40                  45              


Arg Leu Gly Phe Gln Val Trp Leu Lys Asn Gly Val Ile Leu Ser Lys 
    50                  55                  60                  


Leu Val Asn Ser Leu Tyr Pro Asp Gly Ser Lys Pro Val Lys Val Pro 
65                  70                  75                  80  


Glu Asn Pro Pro Ser Met Val Phe Lys Gln Met Glu Gln Val Ala Gln 
                85                  90                  95      


Phe Leu Lys Ala Ala Glu Asp Tyr Gly Val Ile Lys Thr Asp Met Phe 
            100                 105                 110         


Gln Thr Val Asp Leu Phe Glu Gly Lys Asp Met Ala Ala Val Gln Arg 
        115                 120                 125             


Thr Leu Met Ala Leu Gly Ser Leu Ala Val Thr Lys Asn Asp Gly His 
    130                 135                 140                 


Tyr Arg Gly Asp Pro Asn Trp Phe Met Lys Lys Ala Gln Glu His Lys 
145                 150                 155                 160 


Arg Glu Phe Thr Glu Ser Gln Leu Gln Glu Gly Lys His Val Ile Gly 
                165                 170                 175     


Leu Gln Met Gly Ser Asn Arg Gly Ala Ser Gln Ala Gly Met Thr Gly 
            180                 185                 190         


Tyr Gly Arg Pro Arg Gln Ile Ile Ser 
        195                 200     


<210>  68
<211>  377
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human alpha-SMA protein

<400>  68

Met Cys Glu Glu Glu Asp Ser Thr Ala Leu Val Cys Asp Asn Gly Ser 
1               5                   10                  15      


Gly Leu Cys Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val 
            20                  25                  30          


Phe Pro Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly 
        35                  40                  45              


Met Gly Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg 
    50                  55                  60                  


Gly Ile Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Ile Thr Asn 
65                  70                  75                  80  


Trp Asp Asp Met Glu Lys Ile Trp His His Ser Phe Tyr Asn Glu Leu 
                85                  90                  95      


Arg Val Ala Pro Glu Glu His Pro Thr Leu Leu Thr Glu Ala Pro Leu 
            100                 105                 110         


Asn Pro Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr 
        115                 120                 125             


Phe Asn Val Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu 
    130                 135                 140                 


Tyr Ala Ser Gly Arg Thr Thr Gly Ile Val Leu Asp Ser Gly Asp Gly 
145                 150                 155                 160 


Val Thr His Asn Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala 
                165                 170                 175     


Ile Met Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met 
            180                 185                 190         


Lys Ile Leu Thr Glu Arg Gly Tyr Ser Phe Val Thr Thr Ala Glu Arg 
        195                 200                 205             


Glu Ile Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp 
    210                 215                 220                 


Phe Glu Asn Glu Met Ala Thr Ala Ala Ser Ser Ser Ser Leu Glu Lys 
225                 230                 235                 240 


Ser Tyr Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg 
                245                 250                 255     


Phe Arg Cys Pro Glu Thr Leu Phe Gln Pro Ser Phe Ile Gly Met Glu 
            260                 265                 270         


Ser Ala Gly Ile His Glu Thr Thr Tyr Asn Ser Ile Met Lys Cys Asp 
        275                 280                 285             


Ile Asp Ile Arg Lys Asp Leu Tyr Ala Asn Asn Val Leu Ser Gly Gly 
    290                 295                 300                 


Thr Thr Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr 
305                 310                 315                 320 


Ala Leu Ala Pro Ser Thr Met Lys Ile Lys Ile Ile Ala Pro Pro Glu 
                325                 330                 335     


Arg Lys Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser Leu Ser 
            340                 345                 350         


Thr Phe Gln Gln Met Trp Ile Ser Lys Gln Glu Tyr Asp Glu Ala Gly 
        355                 360                 365             


Pro Ser Ile Val His Arg Lys Cys Phe 
    370                 375         


<210>  69
<211>  986
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human MYOCD protein

<400>  69

Met Thr Leu Leu Gly Ser Glu His Ser Leu Leu Ile Arg Ser Lys Phe 
1               5                   10                  15      


Arg Ser Val Leu Gln Leu Arg Leu Gln Gln Arg Arg Thr Gln Glu Gln 
            20                  25                  30          


Leu Ala Asn Gln Gly Ile Ile Pro Pro Leu Lys Arg Pro Ala Glu Phe 
        35                  40                  45              


His Glu Gln Arg Lys His Leu Asp Ser Asp Lys Ala Lys Asn Ser Leu 
    50                  55                  60                  


Lys Arg Lys Ala Arg Asn Arg Cys Asn Ser Ala Asp Leu Val Asn Met 
65                  70                  75                  80  


His Ile Leu Gln Ala Ser Thr Ala Glu Arg Ser Ile Pro Thr Ala Gln 
                85                  90                  95      


Met Lys Leu Lys Arg Ala Arg Leu Ala Asp Asp Leu Asn Glu Lys Ile 
            100                 105                 110         


Ala Leu Arg Pro Gly Pro Leu Glu Leu Val Glu Lys Asn Ile Leu Pro 
        115                 120                 125             


Val Asp Ser Ala Val Lys Glu Ala Ile Lys Gly Asn Gln Val Ser Phe 
    130                 135                 140                 


Ser Lys Ser Thr Asp Ala Phe Ala Phe Glu Glu Asp Ser Ser Ser Asp 
145                 150                 155                 160 


Gly Leu Ser Pro Asp Gln Thr Arg Ser Glu Asp Pro Gln Asn Ser Ala 
                165                 170                 175     


Gly Ser Pro Pro Asp Ala Lys Ala Ser Asp Thr Pro Ser Thr Gly Ser 
            180                 185                 190         


Leu Gly Thr Asn Gln Asp Leu Ala Ser Gly Ser Glu Asn Asp Arg Asn 
        195                 200                 205             


Asp Ser Ala Ser Gln Pro Ser His Gln Ser Asp Ala Gly Lys Gln Gly 
    210                 215                 220                 


Leu Gly Pro Pro Ser Thr Pro Ile Ala Val His Ala Ala Val Lys Ser 
225                 230                 235                 240 


Lys Ser Leu Gly Asp Ser Lys Asn Arg His Lys Lys Pro Lys Asp Pro 
                245                 250                 255     


Lys Pro Lys Val Lys Lys Leu Lys Tyr His Gln Tyr Ile Pro Pro Asp 
            260                 265                 270         


Gln Lys Ala Glu Lys Ser Pro Pro Pro Met Asp Ser Ala Tyr Ala Arg 
        275                 280                 285             


Leu Leu Gln Gln Gln Gln Leu Phe Leu Gln Leu Gln Ile Leu Ser Gln 
    290                 295                 300                 


Gln Gln Gln Gln Gln Gln His Arg Phe Ser Tyr Leu Gly Met His Gln 
305                 310                 315                 320 


Ala Gln Leu Lys Glu Pro Asn Glu Gln Met Val Arg Asn Pro Asn Ser 
                325                 330                 335     


Ser Ser Thr Pro Leu Ser Asn Thr Pro Leu Ser Pro Val Lys Asn Ser 
            340                 345                 350         


Phe Ser Gly Gln Thr Gly Val Ser Ser Phe Lys Pro Gly Pro Leu Pro 
        355                 360                 365             


Pro Asn Leu Asp Asp Leu Lys Val Ser Glu Leu Arg Gln Gln Leu Arg 
    370                 375                 380                 


Ile Arg Gly Leu Pro Val Ser Gly Thr Lys Thr Ala Leu Met Asp Arg 
385                 390                 395                 400 


Leu Arg Pro Phe Gln Asp Cys Ser Gly Asn Pro Val Pro Asn Phe Gly 
                405                 410                 415     


Asp Ile Thr Thr Val Thr Phe Pro Val Thr Pro Asn Thr Leu Pro Asn 
            420                 425                 430         


Tyr Gln Ser Ser Ser Ser Thr Ser Ala Leu Ser Asn Gly Phe Tyr His 
        435                 440                 445             


Phe Gly Ser Thr Ser Ser Ser Pro Pro Ile Ser Pro Ala Ser Ser Asp 
    450                 455                 460                 


Leu Ser Val Ala Gly Ser Leu Pro Asp Thr Phe Asn Asp Ala Ser Pro 
465                 470                 475                 480 


Ser Phe Gly Leu His Pro Ser Pro Val His Val Cys Thr Glu Glu Ser 
                485                 490                 495     


Leu Met Ser Ser Leu Asn Gly Gly Ser Val Pro Ser Glu Leu Asp Gly 
            500                 505                 510         


Leu Asp Ser Glu Lys Asp Lys Met Leu Val Glu Lys Gln Lys Val Ile 
        515                 520                 525             


Asn Glu Leu Thr Trp Lys Leu Gln Gln Glu Gln Arg Gln Val Glu Glu 
    530                 535                 540                 


Leu Arg Met Gln Leu Gln Lys Gln Lys Arg Asn Asn Cys Ser Glu Lys 
545                 550                 555                 560 


Lys Pro Leu Pro Phe Leu Ala Ala Ser Ile Lys Gln Glu Glu Ala Val 
                565                 570                 575     


Ser Ser Cys Pro Phe Ala Ser Gln Val Pro Val Lys Arg Gln Ser Ser 
            580                 585                 590         


Ser Ser Glu Cys His Pro Pro Ala Cys Glu Ala Ala Gln Leu Gln Pro 
        595                 600                 605             


Leu Gly Asn Ala His Cys Val Glu Ser Ser Asp Gln Thr Asn Val Leu 
    610                 615                 620                 


Ser Ser Thr Phe Leu Ser Pro Gln Cys Ser Pro Gln His Ser Pro Leu 
625                 630                 635                 640 


Gly Ala Val Lys Ser Pro Gln His Ile Ser Leu Pro Pro Ser Pro Asn 
                645                 650                 655     


Asn Pro His Phe Leu Pro Ser Ser Ser Gly Ala Gln Gly Glu Gly His 
            660                 665                 670         


Arg Val Ser Ser Pro Ile Ser Ser Gln Val Cys Thr Ala Gln Asn Ser 
        675                 680                 685             


Gly Ala His Asp Gly His Pro Pro Ser Phe Ser Pro His Ser Ser Ser 
    690                 695                 700                 


Leu His Pro Pro Phe Ser Gly Ala Gln Ala Asp Ser Ser His Gly Ala 
705                 710                 715                 720 


Gly Gly Asn Pro Cys Pro Lys Ser Pro Cys Val Gln Gln Lys Met Ala 
                725                 730                 735     


Gly Leu His Ser Ser Asp Lys Val Gly Pro Lys Phe Ser Ile Pro Ser 
            740                 745                 750         


Pro Thr Phe Ser Lys Ser Ser Ser Ala Ile Ser Glu Val Thr Gln Pro 
        755                 760                 765             


Pro Ser Tyr Glu Asp Ala Val Lys Gln Gln Met Thr Arg Ser Gln Gln 
    770                 775                 780                 


Met Asp Glu Leu Leu Asp Val Leu Ile Glu Ser Gly Glu Met Pro Ala 
785                 790                 795                 800 


Asp Ala Arg Glu Asp His Ser Cys Leu Gln Lys Val Pro Lys Ile Pro 
                805                 810                 815     


Arg Ser Ser Arg Ser Pro Thr Ala Val Leu Thr Lys Pro Ser Ala Ser 
            820                 825                 830         


Phe Glu Gln Ala Ser Ser Gly Ser Gln Ile Pro Phe Asp Pro Tyr Ala 
        835                 840                 845             


Thr Asp Ser Asp Glu His Leu Glu Val Leu Leu Asn Ser Gln Ser Pro 
    850                 855                 860                 


Leu Gly Lys Met Ser Asp Val Thr Leu Leu Lys Ile Gly Ser Glu Glu 
865                 870                 875                 880 


Pro His Phe Asp Gly Ile Met Asp Gly Phe Ser Gly Lys Ala Ala Glu 
                885                 890                 895     


Asp Leu Phe Asn Ala His Glu Ile Leu Pro Gly Pro Leu Ser Pro Met 
            900                 905                 910         


Gln Thr Gln Phe Ser Pro Ser Ser Val Asp Ser Asn Gly Leu Gln Leu 
        915                 920                 925             


Ser Phe Thr Glu Ser Pro Trp Glu Thr Met Glu Trp Leu Asp Leu Thr 
    930                 935                 940                 


Pro Pro Asn Ser Thr Pro Gly Phe Ser Ala Leu Thr Thr Ser Ser Pro 
945                 950                 955                 960 


Ser Ile Phe Asn Ile Asp Phe Leu Asp Val Thr Asp Leu Asn Leu Asn 
                965                 970                 975     


Ser Ser Met Asp Leu His Leu Gln Gln Trp 
            980                 985     


<210>  70
<211>  1798
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human alpha-SMA mRNA

<400>  70
ctctccccgc ccccgcgggg cggcgcgcac tcacccaccc gcgccggagc ggacctttgg       60

cttggcttgt cagggcttgt ccaggagttc cgctcctctc tccaaccggg gtccccctcc      120

agcgacccta aagcttccca gacttccgct tcaattcctg tccgcacccc acgcccacct      180

caacgtggag cgcagtggtc tccgaggagc gccggagctg ccccgcctgc ccagcggggt      240

cagcacttcg catcaaggcc caagaaaagc aagtcctcca gcgttctgag cacccgggcc      300

tgagggaagg tcctaacagc ccccgggagc cagtctccaa cgcctcccgc agcagcccgc      360

cgctcccagg tgcccgcgtg cgccgctgcc gccgcaatcc cgcacgcgtc ccgcgcccgc      420

cccactttgc ctatccccgg gactaagacg ggaatcctgt gaagcagctc cagctatgtg      480

tgaagaagag gacagcactg ccttggtgtg tgacaatggc tctgggctct gtaaggccgg      540

ctttgctggg gacgatgctc ccagggctgt tttcccatcc attgtgggac gtcccagaca      600

tcagggggtg atggtgggaa tgggacaaaa agacagctac gtgggtgacg aagcacagag      660

caaaagagga atcctgaccc tgaagtaccc gatagaacat ggcatcatca ccaactggga      720

cgacatggaa aagatctggc accactcttt ctacaatgag cttcgtgttg cccctgaaga      780

gcatcccacc ctgctcacgg aggcacccct gaaccccaag gccaaccggg agaaaatgac      840

tcaaattatg tttgagactt tcaatgtccc agccatgtat gtggctatcc aggcggtgct      900

gtctctctat gcctctggac gcacaactgg catcgtgctg gactctggag atggtgtcac      960

ccacaatgtc cccatctatg agggctatgc cttgccccat gccatcatgc gtctggatct     1020

ggctggccga gatctcactg actacctcat gaagatcctg actgagcgtg gctattcctt     1080

cgttactact gctgagcgtg agattgtccg ggacatcaag gagaaactgt gttatgtagc     1140

tctggacttt gaaaatgaga tggccactgc cgcatcctca tcctcccttg agaagagtta     1200

cgagttgcct gatgggcaag tgatcaccat cggaaatgaa cgtttccgct gcccagagac     1260

cctgttccag ccatccttca tcgggatgga gtctgctggc atccatgaaa ccacctacaa     1320

cagcatcatg aagtgtgata ttgacatcag gaaggacctc tatgctaaca atgtcctatc     1380

agggggcacc actatgtacc ctggcattgc cgaccgaatg cagaaggaga tcacggccct     1440

agcacccagc accatgaaga tcaagatcat tgcccctccg gagcgcaaat actctgtctg     1500

gatcggtggc tccatcctgg cctctctgtc caccttccag cagatgtgga tcagcaaaca     1560

ggaatacgat gaagccgggc cttccattgt ccaccgcaaa tgcttctaaa acactttcct     1620

gctcctctct gtctctagca cacaactgtg aatgtcctgt ggaattatgc cttcagttct     1680

tttccaaatc attcctagcc aaagctctga ctcgttacct atgtgttttt taataaatct     1740

gaaataggct actggtaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa       1798


<210>  71
<211>  8580
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human MYOCD mRNA

<400>  71
aatcgccggc agcctatgac atcagacagg aacgcctggg atgccgcgct gctcctggcc       60

aacctccgag gaggaggagg gtcccgccgg ctaagagtta attagccccg cacggcgagg      120

ggggaggcgc cagttttctg gggacactgg ctgccactgt actcctaccc aggggagctc      180

acggagagtt ggatgaattc tgggttgtta gctgcggtca gctgggctcc cgggagcctg      240

ttgctggtgg agaacagggg gcgcctggcc aagggaccag cggcttgctg agactcaaca      300

tgacactcct ggggtctgag cattccttgc tgattaggag caagttcaga tcagttttac      360

agttaagact tcaacaaaga aggacccagg aacaactggc taaccaaggc ataataccac      420

cactgaaacg tccagctgaa ttccatgagc aaagaaaaca tttggatagt gacaaggcta      480

aaaattccct gaagcgcaaa gccagaaaca ggtgcaacag tgccgacttg gttaatatgc      540

acatactcca agcttccact gcagagaggt ccattccaac tgctcagatg aagctgaaaa      600

gagcccgact cgccgatgat ctcaatgaaa aaattgctct acgaccaggg ccactggagc      660

tggtggaaaa aaacattctt cctgtggatt ctgctgtgaa agaggccata aaaggtaacc      720

aggtgagttt ctccaaatcc acggatgctt ttgcctttga agaggacagc agcagcgatg      780

ggctttctcc ggatcagact cgaagtgaag acccccaaaa ctcagcggga tccccgccag      840

acgctaaagc ctcagatacc ccttcgacag gttctctggg gacaaaccag gatcttgctt      900

ctggctcaga aaatgacaga aatgactcag cctcacagcc cagccaccag tcagatgcgg      960

ggaagcaggg gcttggcccc cccagcaccc ccatagccgt gcatgctgct gtaaagtcca     1020

aatccttggg tgacagtaag aaccgccaca aaaagcccaa ggaccccaag ccaaaggtga     1080

agaagcttaa atatcaccag tacattcccc cagaccagaa ggcagagaag tcccctccac     1140

ctatggactc agcctacgct cggctgctcc agcaacagca gctgttcctg cagctccaaa     1200

tcctcagcca gcagcagcag cagcagcaac accgattcag ctacctaggg atgcaccaag     1260

ctcagcttaa ggaaccaaat gaacagatgg tcagaaatcc aaactcttct tcaacgccac     1320

tgagcaatac ccccttgtct cctgtcaaaa acagtttttc tggacaaact ggtgtctctt     1380

ctttcaaacc aggcccactc ccacctaacc tggatgatct gaaggtctct gaattaagac     1440

aacagcttcg aattcggggc ttgcctgtgt caggcaccaa aacggctctc atggaccggc     1500

ttcgaccctt ccaggactgc tctggcaacc cagtgccgaa ctttggggat ataacgactg     1560

tcacttttcc tgtcacaccc aacacgctgc ccaattacca gtcttcctct tctaccagtg     1620

ccctgtccaa cggcttctac cactttggca gcaccagctc cagccccccg atctccccag     1680

cctcctctga cctgtcagtc gctgggtccc tgccggacac cttcaatgat gcctccccct     1740

ccttcggcct gcacccgtcc ccagtccacg tgtgcacgga ggaaagtctc atgagcagcc     1800

tgaatggggg ctctgttcct tctgagctgg atgggctgga ctccgagaag gacaagatgc     1860

tggtggagaa gcagaaggtg atcaatgaac tcacctggaa actccagcaa gagcagaggc     1920

aggtggagga gctgaggatg cagcttcaga agcagaaaag gaataactgt tcagagaaga     1980

agccgctgcc tttcctggct gcctccatca agcaggaaga ggctgtctcc agctgtcctt     2040

ttgcatccca agtacctgtg aaaagacaaa gcagcagctc agagtgtcac ccaccggctt     2100

gtgaagctgc tcaactccag cctcttggaa atgctcattg tgtggagtcc tcagatcaaa     2160

ccaatgtact ttcttccaca tttctcagcc cccagtgttc ccctcagcat tcaccgctgg     2220

gggctgtgaa aagcccacag cacatcagtt tgcccccatc acccaacaac cctcactttc     2280

tgccctcatc ctccggggcc cagggagaag ggcacagggt ctcctcgccc atcagcagcc     2340

aggtgtgcac tgcacagaac tcaggagcac acgatggcca tcctccaagc ttctctcccc     2400

attcttccag cctccacccg cccttctctg gagcccaagc agacagcagt catggtgccg     2460

ggggaaaccc ttgtcccaaa agcccatgtg tacagcaaaa gatggctggt ttacactctt     2520

ctgataaggt ggggccaaag ttttcaattc catccccaac tttttctaag tcaagttcag     2580

caatttcaga ggtaacacag cctccatcct atgaagatgc cgtaaagcag caaatgaccc     2640

ggagtcagca gatggatgaa ctcctggacg tgcttattga aagcggagaa atgccagcag     2700

acgctagaga ggatcactca tgtcttcaaa aagtcccaaa gatacccaga tcttcccgaa     2760

gtccaactgc tgtcctcacc aagccctcgg cttcctttga acaagcctct tcaggcagcc     2820

agatcccctt tgatccctat gccaccgaca gtgatgagca tcttgaagtc ttattaaatt     2880

cccagagccc cctaggaaag atgagtgatg tcacccttct aaaaattggg agcgaagagc     2940

ctcactttga tgggataatg gatggattct ctgggaaggc tgcagaagac ctcttcaatg     3000

cacatgagat cttgccaggc cccctctctc caatgcagac acagttttca ccctcttctg     3060

tggacagcaa tgggctgcag ttaagcttca ctgaatctcc ctgggaaacc atggagtggc     3120

tggacctcac tccgccaaat tccacaccag gctttagcgc cctcaccacc agcagcccca     3180

gcatcttcaa catcgatttc ctggatgtca ctgatctcaa tttgaattct tccatggacc     3240

ttcacttgca gcagtggtag aatgcccaat gcaccagtgc tatggaagac caatggagtt     3300

ccatggggga aagcacacag ccatacatac tttactgtcc aaaaacagaa gaagaagaag     3360

agaattaaaa agaagcaatg atttctgtgc caatgaacaa gaacaaaagt catttttaga     3420

aatacatata ctgtaatatt taccaacagt cagtaactgt taatgatttc aacaatgcat     3480

taaaagaatg tgctttctca gattaaggat gccaaaaaag atatttcact gccttttcaa     3540

agaccagtat attttctagc ccataatttt tctcaggcat tgttggggca taagctcaca     3600

ctgtaagctt ttctcatgaa ttcactagac ataacgtgga aggaaaacgt agtcttttgg     3660

gagtacaggg aagccagccc ctcaaagctt atggaagaca tacctgcaat ggaagctgtt     3720

gcccaatgtc tccattacta tctttcaaaa gagaagccag acccagcttc agatcaaaag     3780

ttcttgagac agaggaacaa aaccaatcga tttccaggga agctaatcaa ctctcttttc     3840

cctctaccac aaaactgccc tgctggagtg gttctgaacc tgtacccagg actcgatgtg     3900

gtcactaata acaattaacc tgaactgagt ccacagaact ccactcggaa ctttcttctt     3960

ttttaactag tggcccaatc attcccacca tctctgtgct gataagtacg tgtcctagat     4020

gagaaccctg aagaatgcag accttcttcc cccgaaggag atgccacaag ctctccaaca     4080

cagccccctt tagttccaaa gactagagat gaccacattg gtagaagtat atctcgaggc     4140

acaggaaggg agccccacca gggataattc agacaggact agagaataac atcatttcac     4200

ataccctggg ataaacaccc tgggttccta tagaaggact attacttatg ggagtccaac     4260

ttctcctttt gttttgttat tatcagttta tctttctccc actccacttt tccttcaagg     4320

taccaatcct ttcctgttcc tcgtttggcc atctttcttt ttctgcctcc acattgggag     4380

gggaggactt ctcagttcta acaagctgcc atactcctaa gaaagccatt tttgaaaaat     4440

ttaacaatcc aggttcttct ggagaactca ttctccacac gcacagtttg ctgcaaaagg     4500

aagttgcaag aatttcttga ggaagaaact ggtgacttgg tccatcagtc acgaagttct     4560

ttctattctc gtttagtttt caagaaatta ttggtttgtg ttgctctggg gaaattggaa     4620

atcattacat tgtaaagaca aatatggatg atatttacaa gagagaattt cagatctggg     4680

tttttgaaag aaaacagaat tgcgcattga aaacgatgga aggaaaaaga caatggtcta     4740

atgtgcattc ctcattacct ctcgtggctt tggctgggag ttggaaaaag ctaaaatttc     4800

agaacagtct ctgtaaggct ctctgtggct ccagttcacc attttatatt gttgcatgct     4860

gtagaaagga gctattgctg ttgttttgtt tttttattta aatcactaag gcactgtttt     4920

tatcttttgt aaaaaaaaaa aaaaagttgt tcactgtgca cttatagaaa aaataatcaa     4980

aaatgttggg attttagaag ctctcttttt gataaaccaa agatttagaa gtcattccat     5040

tgttaacttg taaaaatgtg tgaacacaga gagtttttgg tgattgctac tctgaaagct     5100

gccagatctt attctggggg tgggatgtgg aggaatacac atacacacac aaacatacat     5160

gtatgtataa tagatatata catatgtgta tattatatct gtgtgtgcat gtatctccaa     5220

aagcggcgtt acagagttct acaccaaaag cctttaaccc ttaatctgct gtgaatgata     5280

cctggccttt ctcactatga atttctgatt aaccaaccag actacacgtt gcctctctgt     5340

gtatgactaa cggctccaac ccgatgactc acagctactt gcttatcgtg aacaagctca     5400

tcttggcaat gaatatggat gtgaaaagac agaacagctt caccattagt agctggaaat     5460

ggtatcacag tctcttatag aggaatatga aaggaacaag aaaatcattt tacattcctt     5520

ttatctgtat tgtgctttaa aagatccaca tggtaaattt tttattttgc ttttatgtca     5580

gtcatcagaa ccaaaaaaat ccagaagaaa aaattgccag tgtttccttt gaagatgaag     5640

ctactgggga agaaaacctt attaatacac tccacacatt tgttcattcc tcagctgttg     5700

gtgttttctt ggggtcttga caaagcttgc tggtcagtgc acttttcagg tgtcacgttt     5760

tgctgtttgt atgttttttc ttccccttac ttcctttgga aaacaaactc acacagtgcc     5820

cctactctga gacctgggac tgagtgttaa ttattttttc cttgggtatt tctatctgag     5880

agactagacc tagttaggag gcctctgtac ttctccagat tgtacctttt tatggggatc     5940

tttgaggcta tgacccagga ctgatagata tgccttacgg aagacaaaag ataaaatggt     6000

tcctatatcc taatgcaaac caacacagtt aaaagagcag atctctggat aactgctctc     6060

aacctgcttc tacagtctcc acaaaccgca ttcaccctct ctcttcatag ctcagacatg     6120

aaatttgagg gagaaaactg gagataattg ggagaaaatt gatgaagttg gctgcttcca     6180

gtagatcaga taatccatga atttgtctcc cattgagaat tttattttaa attcttttaa     6240

actcttcgtt gtgtcttttg tgatgacaaa tcaggcatga ctaaaagatg tacagagact     6300

tacgaagatg gtcacattca agttccctaa tgctcttaga acctgaagat gaccatgtgt     6360

agttttctta agacctctga acccccatgg tgatgaagac ttgaagacat ttgcagctat     6420

ctgctgcagt ctggtagatt catacttatc taaagaagtc aaaaaattta ttcgtgcaag     6480

tgcttgcagg aagccagtgc ttattagtag tgaccctgct tctatcaacg ttattgagac     6540

aacacatatt ctattctaag ggagaaagag ggaggaagag agggagggag ggaggaagaa     6600

gagggaggga gcgaggaagg aagataggag atgggtaggg gggtaaagag aaaaggaggg     6660

agaagggaag gaaggaaaga agagaggaaa gaaaggaggg aaggaaaaaa gggccaaact     6720

ttctgatcta tgaacttctc agttcagctg tcacattatg agaagtaaat cagaattttt     6780

ttaaggagaa gtcattctta gcactacaat aattgtacca gtaattgagg aaaccaagac     6840

aatcttcacc tgaataatag agggtctgag aactgtcagc cttttgccat tcaaaaacat     6900

ttatgtccaa cctgaaaaaa aagcatcaat aaaacctatc ccaagcattc aaaatagtcc     6960

tttccaaatg ttatttattt taaagtcaat cagctctttt agaaacagat tctggtctgg     7020

ctgaaaactc ccacaacaaa tttactcatc cagtggctaa tatttaatgc ccaccatggg     7080

cagagcacac aaatcttcag ataaacaata ctgaattgat tcagcacaga ttgttcagat     7140

ttgaatgacc agggagttgt atttgcacat gcaagaacac taagaatctc ccagtcctca     7200

aactagaaac ctttcagcta cgatgaaaaa aaaaaagggg tcttcatttt tccaagaggg     7260

ggtggaggtg gggatcactt tttagctaaa agctatctct cacttcaaaa ttcttgtctt     7320

tttctttgtg gacaaacacc agtagtctat cacttggaga tcttttaata tctcccatca     7380

tttaaaacat ccacgagagt ttgaagattt gtgttgattg ccagatacag aagccccttg     7440

aaaataagga aagggtggag gaagcatttt tgtgtcctat ccctacttat cgtagcagct     7500

ctatagacaa aagggacact tactggtgag cctctggccc ttaaaagaaa atcatctaag     7560

aatatgaagg caatttgatt tccccccaca gccctcagct gccttcctca cagaaggaag     7620

ttcccaaaat tgctggtaca cagtttgcaa tcaaatatca gatatgagaa aacctgtagt     7680

gaagagtctg ggttcttggt tttctcataa atccaatata aatttgtagg ttggttcagg     7740

gtcaaaattg ccagtgcttt attagacaga tgatactgat agacacacag agcccaggtc     7800

ctggaacaag acaatcctgt agtgccaaga tctggtcagt tgcgttaagg agctgggttt     7860

gattctagag tccaggttta tagagaaacc ctggctagat tgagcctacc catggggaga     7920

cgatttcaag acaggatgag atctgggaag aattttgttg tcatctgcca gggaaattat     7980

cacaggactc attgaatgca ataacatgtg agtaagttcc cttttgattc tgggaatcag     8040

cgattttccc tgtggattaa gacaaaccaa cgccagaagg tctcctgtgc ttattttaac     8100

catctgctcc catcgtgaac cctggagcat gcatttccta gaagtggttt catagctcct     8160

gtgtgttcat ggaaaagggg agtataatga tggggatgct ggaagctttt ttaatgtttt     8220

ccaaaggaaa ggaacccaca ctgctcccca gagttccttt ccaatggccc tgcagtaaga     8280

acggaggaca atgtattgct gggtgcttaa aatcctccct cagtgaagca caaagagaca     8340

ctttgtaaag aaaaaaagag caagcatagg ttctctgtgg gaccttgtgg agtggtgttt     8400

tcacgttggt ctctttggct caattgagca taatcagaaa gaaatgtggg ttattgggaa     8460

gagacaaaaa gcagtggcta aaataccaaa gttggcatgt gttctttttt aaaaaaaaaa     8520

aaaaatgcat atatttttaa ataaaatgtt tattttaaaa agaaaaaaaa aaaaaaaaaa     8580


<210>  72
<211>  524
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human BSAP protein

<400>  72

Met Ile Ser Pro Phe Leu Val Leu Ala Ile Gly Thr Cys Leu Thr Asn 
1               5                   10                  15      


Ser Leu Val Pro Glu Lys Glu Lys Asp Pro Lys Tyr Trp Arg Asp Gln 
            20                  25                  30          


Ala Gln Glu Thr Leu Lys Tyr Ala Leu Glu Leu Gln Lys Leu Asn Thr 
        35                  40                  45              


Asn Val Ala Lys Asn Val Ile Met Phe Leu Gly Asp Gly Met Gly Val 
    50                  55                  60                  


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Leu His His Asn 
65                  70                  75                  80  


Pro Gly Glu Glu Thr Arg Leu Glu Met Asp Lys Phe Pro Phe Val Ala 
                85                  90                  95      


Leu Ser Lys Thr Tyr Asn Thr Asn Ala Gln Val Pro Asp Ser Ala Gly 
            100                 105                 110         


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Ala Asn Glu Gly Thr Val 
        115                 120                 125             


Gly Val Ser Ala Ala Thr Glu Arg Ser Arg Cys Asn Thr Thr Gln Gly 
    130                 135                 140                 


Asn Glu Val Thr Ser Ile Leu Arg Trp Ala Lys Asp Ala Gly Lys Ser 
145                 150                 155                 160 


Val Gly Ile Val Thr Thr Thr Arg Val Asn His Ala Thr Pro Ser Ala 
                165                 170                 175     


Ala Tyr Ala His Ser Ala Asp Arg Asp Trp Tyr Ser Asp Asn Glu Met 
            180                 185                 190         


Pro Pro Glu Ala Leu Ser Gln Gly Cys Lys Asp Ile Ala Tyr Gln Leu 
        195                 200                 205             


Met His Asn Ile Arg Asp Ile Asp Val Ile Met Gly Gly Gly Arg Lys 
    210                 215                 220                 


Tyr Met Tyr Pro Lys Asn Lys Thr Asp Val Glu Tyr Glu Ser Asp Glu 
225                 230                 235                 240 


Lys Ala Arg Gly Thr Arg Leu Asp Gly Leu Asp Leu Val Asp Thr Trp 
                245                 250                 255     


Lys Ser Phe Lys Pro Arg Tyr Lys His Ser His Phe Ile Trp Asn Arg 
            260                 265                 270         


Thr Glu Leu Leu Thr Leu Asp Pro His Asn Val Asp Tyr Leu Leu Gly 
        275                 280                 285             


Leu Phe Glu Pro Gly Asp Met Gln Tyr Glu Leu Asn Arg Asn Asn Val 
    290                 295                 300                 


Thr Asp Pro Ser Leu Ser Glu Met Val Val Val Ala Ile Gln Ile Leu 
305                 310                 315                 320 


Arg Lys Asn Pro Lys Gly Phe Phe Leu Leu Val Glu Gly Gly Arg Ile 
                325                 330                 335     


Asp His Gly His His Glu Gly Lys Ala Lys Gln Ala Leu His Glu Ala 
            340                 345                 350         


Val Glu Met Asp Arg Ala Ile Gly Gln Ala Gly Ser Leu Thr Ser Ser 
        355                 360                 365             


Glu Asp Thr Leu Thr Val Val Thr Ala Asp His Ser His Val Phe Thr 
    370                 375                 380                 


Phe Gly Gly Tyr Thr Pro Arg Gly Asn Ser Ile Phe Gly Leu Ala Pro 
385                 390                 395                 400 


Met Leu Ser Asp Thr Asp Lys Lys Pro Phe Thr Ala Ile Leu Tyr Gly 
                405                 410                 415     


Asn Gly Pro Gly Tyr Lys Val Val Gly Gly Glu Arg Glu Asn Val Ser 
            420                 425                 430         


Met Val Asp Tyr Ala His Asn Asn Tyr Gln Ala Gln Ser Ala Val Pro 
        435                 440                 445             


Leu Arg His Glu Thr His Gly Gly Glu Asp Val Ala Val Phe Ser Lys 
    450                 455                 460                 


Gly Pro Met Ala His Leu Leu His Gly Val His Glu Gln Asn Tyr Val 
465                 470                 475                 480 


Pro His Val Met Ala Tyr Ala Ala Cys Ile Gly Ala Asn Leu Gly His 
                485                 490                 495     


Cys Ala Pro Ala Ser Ser Ala Gly Ser Leu Ala Ala Gly Pro Leu Leu 
            500                 505                 510         


Leu Ala Leu Ala Leu Tyr Pro Leu Ser Val Leu Phe 
        515                 520                 


<210>  73
<211>  2606
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human BSAP mRNA

<400>  73
ccgggcctca ctcgggcccc gcggccgcct ttataaggcg gcgggggtgg tggcccgggc       60

cgcgttgcgc tcccgccact ccgcgcccgc tatcctggct ccgtgctccc acgcgcttgt      120

gcctggacgg accctcgcca gtgctctgcg caggattgga acatcagtta acatctgacc      180

actgccagcc caccccctcc cacccacgtc gattgcatct ctgggctcca gggataaagc      240

aggtcttggg gtgcaccatg atttcaccat tcttagtact ggccattggc acctgcctta      300

ctaactcctt agtgccagag aaagagaaag accccaagta ctggcgagac caagcgcaag      360

agacactgaa atatgccctg gagcttcaga agctcaacac caacgtggct aagaatgtca      420

tcatgttcct gggagatggg atgggtgtct ccacagtgac ggctgcccgc atcctcaagg      480

gtcagctcca ccacaaccct ggggaggaga ccaggctgga gatggacaag ttccccttcg      540

tggccctctc caagacgtac aacaccaatg cccaggtccc tgacagcgcc ggcaccgcca      600

ccgcctacct gtgtggggtg aaggccaatg agggcaccgt gggggtaagc gcagccactg      660

agcgttcccg gtgcaacacc acccagggga acgaggtcac ctccatcctg cgctgggcca      720

aggacgctgg gaaatctgtg ggcattgtga ccaccacgag agtgaaccat gccaccccca      780

gcgccgccta cgcccactcg gctgaccggg actggtactc agacaacgag atgccccctg      840

aggccttgag ccagggctgt aaggacatcg cctaccagct catgcataac atcagggaca      900

ttgacgtgat catggggggt ggccggaaat acatgtaccc caagaataaa actgatgtgg      960

agtatgagag tgacgagaaa gccaggggca cgaggctgga cggcctggac ctcgttgaca     1020

cctggaagag cttcaaaccg agatacaagc actcccactt catctggaac cgcacggaac     1080

tcctgaccct tgacccccac aatgtggact acctattggg tctcttcgag ccaggggaca     1140

tgcagtacga gctgaacagg aacaacgtga cggacccgtc actctccgag atggtggtgg     1200

tggccatcca gatcctgcgg aagaacccca aaggcttctt cttgctggtg gaaggaggca     1260

gaattgacca cgggcaccat gaaggaaaag ccaagcaggc cctgcatgag gcggtggaga     1320

tggaccgggc catcgggcag gcaggcagct tgacctcctc ggaagacact ctgaccgtgg     1380

tcactgcgga ccattcccac gtcttcacat ttggtggata caccccccgt ggcaactcta     1440

tctttggtct ggcccccatg ctgagtgaca cagacaagaa gcccttcact gccatcctgt     1500

atggcaatgg gcctggctac aaggtggtgg gcggtgaacg agagaatgtc tccatggtgg     1560

actatgctca caacaactac caggcgcagt ctgctgtgcc cctgcgccac gagacccacg     1620

gcggggagga cgtggccgtc ttctccaagg gccccatggc gcacctgctg cacggcgtcc     1680

acgagcagaa ctacgtcccc cacgtgatgg cgtatgcagc ctgcatcggg gccaacctcg     1740

gccactgtgc tcctgccagc tcggcaggca gccttgctgc aggccccctg ctgctcgcgc     1800

tggccctcta ccccctgagc gtcctgttct gagggcccag ggcccgggca cccacaagcc     1860

cgtgacagat gccaacttcc cacacggcag cccccccctc aaggggcagg gaggtggggg     1920

cctcctcagc ctctgcaact gcaagaaagg ggacccaaga aaccaaagtc tgccgcccac     1980

ctcgctcccc tctggaatct tccccaaggg ccaaacccac ttctggcctc cagcctttgc     2040

tccctccccg ctgccctttg gccaacaggg tagatttctc ttgggcaggc agagagtaca     2100

gactgcagac attctcaaag cctcttattt ttctagcgaa cgtatttctc cagacccaga     2160

ggccctgaag cctccgtgga acattctgga tctgaccctc ccagtctcat ctcctgaccc     2220

tcccactccc atctccttac ctctggaacc ccccaggccc tacaatgctc atgtccctgt     2280

ccccaggccc agccctcctt caggggagtt gaggtctttc tcctcaggac aaggccttgc     2340

tcactcactc actccaagac caccagggtc ccaggaagcc ggtgcctggg tggccatcct     2400

acccagcgtg gcccaggccg ggaagagcca cctggcaggg ctcacactcc tgggctctga     2460

acacacacgc cagctcctct ctgaagcgac tctcctgttt ggaacggcaa aaaaaaattt     2520

ttttttctct ttttggtggt ggttaaaagg gaacacaaaa catttaaata aaactttcca     2580

aatatttccg aggacaaaaa aaaaaa                                          2606


<210>  74
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Snai1 mRNA

<400>  74
attcattgcg ccgcggcacg gcctagcgag tggttcttct gcgctactgc tgcgcgaatc       60

ggcgacccca gtgcctcgac cactatgccg cgctctttcc tcgtcaggaa gccctccgac      120

cccaatcgga agcctaacta cagcgagctg caggactcta atccagagtt taccttccag      180

cagccctacg accaggccca cctgctggca gccatcccac ctccggagat cctcaacccc      240

accgcctcgc tgccaatgct catctgggac tctgtcctgg cgccccaagc ccagccaatt      300

gcctgggcct cccttcggct ccaggagagt cccagggtgg cagagctgac ctccctgtca      360

gatgaggaca gtgggaaagg ctcccagccc cccagcccac cctcaccggc tccttcgtcc      420

ttctcctcta cttcagtctc ttccttggag gccgaggcct atgctgcctt cccaggcttg      480

ggccaagtgc ccaagcagct ggcccagctc tctgaggcca aggatctcca ggctcgaaag      540

gccttcaact gcaaatactg caacaaggaa tacctcagcc tgggtgccct caagatgcac      600

atccgaagcc acacgctgcc ctgcgtctgc ggaacctgcg ggaaggcctt ctctaggccc      660

tggctgctac aaggccatgt ccggacccac actggcgaga agcccttctc ctgtccccac      720

tgcagccgtg ccttcgctga ccgctccaac ctgcgggccc acctccagac ccactcagat      780

gtcaagaagt accagtgcca ggcgtgtgct cggaccttct cccgaatgtc cctgctccac      840

aagcaccaag agtccggctg ctcaggatgt ccccgctgac cctcgaggct ccctcttcct      900

ctccatacct gcccctgcct gacagccttc cccagctcca gcaggaagga ccccacatcc      960

ttctcactgc catggaattc cctcctgagt gccccacttc tggccacatc agccccacag     1020

gactttgatg aagaccattt tctggttctg tgtcctctgc ctgggctctg gaagaggcct     1080

tcccatggcc atttctgtgg agggagggca gctggccccc agccctgggg gattcctgag     1140

ctggcctgtc tgcgtgggtt tttgtatcca gagctgtttg gatacagctg ctttgagcta     1200

caggacaaag gctgacagac tcactgggaa gctcccaccc cactcagggg accccactcc     1260

cctcacacac acccccccac aaggaaccct caggccaccc tccacgaggt gtgactaact     1320

atgcaataat ccacccccag gtgcagcccc agggcctgcg gaggcggtgg cagactagag     1380

tctgagatgc cccgagccca ggcagctatt tcagcctcct gtttggtggg gtggcacctg     1440

tttcccgggc aatttaacaa tgtctgaaaa gggactgtga gtaatggctg tcacttgtcg     1500

ggggcccaag tggggtgctc tggtctgacc gatgtgtctc ccagaactat tctgggggcc     1560

cgacaggtgg gcctgggagg aagatgttta catttttaaa ggtacactgg tatttatatt     1620

tcaaacattt tgtatcaagg aaacgttttg tatagttata tgtacagttt attgatattc     1680

aataaagcag ttaatttata tattaaaaaa aaaaaaaaaa aa                        1722


<210>  75
<211>  1815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dkk1 mRNA

<400>  75
gcagagctct gtgctccctg cagtcaggac tctgggaccg cagggggctc ccggaccctg       60

actctgcagc cgaaccggca cggtttcgtg gggacccagg cttgcaaagt gacggtcatt      120

ttctctttct ttctccctct tgagtccttc tgagatgatg gctctgggcg cagcgggagc      180

tacccgggtc tttgtcgcga tggtagcggc ggctctcggc ggccaccctc tgctgggagt      240

gagcgccacc ttgaactcgg ttctcaattc caacgctatc aagaacctgc ccccaccgct      300

gggcggcgct gcggggcacc caggctctgc agtcagcgcc gcgccgggaa tcctgtaccc      360

gggcgggaat aagtaccaga ccattgacaa ctaccagccg tacccgtgcg cagaggacga      420

ggagtgcggc actgatgagt actgcgctag tcccacccgc ggaggggacg caggcgtgca      480

aatctgtctc gcctgcagga agcgccgaaa acgctgcatg cgtcacgcta tgtgctgccc      540

cgggaattac tgcaaaaatg gaatatgtgt gtcttctgat caaaatcatt tccgaggaga      600

aattgaggaa accatcactg aaagctttgg taatgatcat agcaccttgg atgggtattc      660

cagaagaacc accttgtctt caaaaatgta tcacaccaaa ggacaagaag gttctgtttg      720

tctccggtca tcagactgtg cctcaggatt gtgttgtgct agacacttct ggtccaagat      780

ctgtaaacct gtcctgaaag aaggtcaagt gtgtaccaag cataggagaa aaggctctca      840

tggactagaa atattccagc gttgttactg tggagaaggt ctgtcttgcc ggatacagaa      900

agatcaccat caagccagta attcttctag gcttcacact tgtcagagac actaaaccag      960

ctatccaaat gcagtgaact ccttttatat aatagatgct atgaaaacct tttatgacct     1020

tcatcaactc aatcctaagg atatacaagt tctgtggttt cagttaagca ttccaataac     1080

accttccaaa aacctggagt gtaagagctt tgtttcttta tggaactccc ctgtgattgc     1140

agtaaattac tgtattgtaa attctcagtg tggcacttac ctgtaaatgc aatgaaactt     1200

ttaattattt ttctaaaggt gctgcactgc ctatttttcc tcttgttatg taaatttttg     1260

tacacattga ttgttatctt gactgacaaa tattctatat tgaactgaag taaatcattt     1320

cagcttatag ttcttaaaag cataaccctt taccccattt aattctagag tctagaacgc     1380

aaggatctct tggaatgaca aatgataggt acctaaaatg taacatgaaa atactagctt     1440

attttctgaa atgtactatc ttaatgctta aattatattt ccctttaggc tgtgatagtt     1500

tttgaaataa aatttaacat ttaatatcat gaaatgttat aagtagacat acattttggg     1560

attgtgatct tagaggtttg tgtgtgtgta cgtatgtgtg tgttctacaa gaacggaagt     1620

gtgatatgtt taaagatgat cagagaaaag acagtgtcta aatataagac aatattgatc     1680

agctctagaa taactttaaa gaaagacgtg ttctgcattg ataaactcaa atgatcatgg     1740

cagaatgaga gtgaatctta cattactact ttcaaaaata gtttccaata aattaataat     1800

acctaaaaaa aaaaa                                                      1815


<210>  76
<211>  5927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Col1a1 mRNA

<400>  76
tcgtcggagc agacgggagt ttctcctcgg ggtcggagca ggaggcacgc ggagtgtgag       60

gccacgcatg agcggacgct aaccccctcc ccagccacaa agagtctaca tgtctagggt      120

ctagacatgt tcagctttgt ggacctccgg ctcctgctcc tcttagcggc caccgccctc      180

ctgacgcacg gccaagagga aggccaagtc gagggccaag acgaagacat cccaccaatc      240

acctgcgtac agaacggcct caggtaccat gaccgagacg tgtggaaacc cgagccctgc      300

cggatctgcg tctgcgacaa cggcaaggtg ttgtgcgatg acgtgatctg tgacgagacc      360

aagaactgcc ccggcgccga agtccccgag ggcgagtgct gtcccgtctg ccccgacggc      420

tcagagtcac ccaccgacca agaaaccacc ggcgtcgagg gacccaaggg agacactggc      480

ccccgaggcc caaggggacc cgcaggcccc cctggccgag atggcatccc tggacagcct      540

ggacttcccg gaccccccgg accccccgga cctcccggac cccctggcct cggaggaaac      600

tttgctcccc agctgtctta tggctatgat gagaaatcaa ccggaggaat ttccgtgcct      660

ggccccatgg gtccctctgg tcctcgtggt ctccctggcc cccctggtgc acctggtccc      720

caaggcttcc aaggtccccc tggtgagcct ggcgagcctg gagcttcagg tcccatgggt      780

ccccgaggtc ccccaggtcc ccctggaaag aatggagatg atggggaagc tggaaaacct      840

ggtcgtcctg gtgagcgtgg gcctcctggg cctcagggtg ctcgaggatt gcccggaaca      900

gctggcctcc ctggaatgaa gggacacaga ggtttcagtg gtttggatgg tgccaaggga      960

gatgctggtc ctgctggtcc taagggtgag cctggcagcc ctggtgaaaa tggagctcct     1020

ggtcagatgg gcccccgtgg cctgcctggt gagagaggtc gccctggagc ccctggccct     1080

gctggtgctc gtggaaatga tggtgctact ggtgctgccg ggccccctgg tcccaccggc     1140

cccgctggtc ctcctggctt ccctggtgct gttggtgcta agggtgaagc tggtccccaa     1200

gggccccgag gctctgaagg tccccagggt gtgcgtggtg agcctggccc ccctggccct     1260

gctggtgctg ctggccctgc tggaaaccct ggtgctgatg gacagcctgg tgctaaaggt     1320

gccaatggtg ctcctggtat tgctggtgct cctggcttcc ctggtgcccg aggcccctct     1380

ggaccccagg gccccggcgg ccctcctggt cccaagggta acagcggtga acctggtgct     1440

cctggcagca aaggagacac tggtgctaag ggagagcctg gccctgttgg tgttcaagga     1500

ccccctggcc ctgctggaga ggaaggaaag cgaggagctc gaggtgaacc cggacccact     1560

ggcctgcccg gaccccctgg cgagcgtggt ggacctggta gccgtggttt ccctggcgca     1620

gatggtgttg ctggtcccaa gggtcccgct ggtgaacgtg gttctcctgg ccctgctggc     1680

cccaaaggat ctcctggtga agctggtcgt cccggtgaag ctggtctgcc tggtgccaag     1740

ggtctgactg gaagccctgg cagccctggt cctgatggca aaactggccc ccctggtccc     1800

gccggtcaag atggtcgccc cggaccccca ggcccacctg gtgcccgtgg tcaggctggt     1860

gtgatgggat tccctggacc taaaggtgct gctggagagc ccggcaaggc tggagagcga     1920

ggtgttcccg gaccccctgg cgctgtcggt cctgctggca aagatggaga ggctggagct     1980

cagggacccc ctggccctgc tggtcccgct ggcgagagag gtgaacaagg ccctgctggc     2040

tcccccggat tccagggtct ccctggtcct gctggtcctc caggtgaagc aggcaaacct     2100

ggtgaacagg gtgttcctgg agaccttggc gcccctggcc cctctggagc aagaggcgag     2160

agaggtttcc ctggcgagcg tggtgtgcaa ggtccccctg gtcctgctgg tccccgaggg     2220

gccaacggtg ctcccggcaa cgatggtgct aagggtgatg ctggtgcccc tggagctccc     2280

ggtagccagg gcgcccctgg ccttcaggga atgcctggtg aacgtggtgc agctggtctt     2340

ccagggccta agggtgacag aggtgatgct ggtcccaaag gtgctgatgg ctctcctggc     2400

aaagatggcg tccgtggtct gactggcccc attggtcctc ctggccctgc tggtgcccct     2460

ggtgacaagg gtgaaagtgg tcccagcggc cctgctggtc ccactggagc tcgtggtgcc     2520

cccggagacc gtggtgagcc tggtcccccc ggccctgctg gctttgctgg cccccctggt     2580

gctgacggcc aacctggtgc taaaggcgaa cctggtgatg ctggtgctaa aggcgatgct     2640

ggtccccctg gccctgccgg acccgctgga ccccctggcc ccattggtaa tgttggtgct     2700

cctggagcca aaggtgctcg cggcagcgct ggtccccctg gtgctactgg tttccctggt     2760

gctgctggcc gagtcggtcc tcctggcccc tctggaaatg ctggaccccc tggccctcct     2820

ggtcctgctg gcaaagaagg cggcaaaggt ccccgtggtg agactggccc tgctggacgt     2880

cctggtgaag ttggtccccc tggtccccct ggccctgctg gcgagaaagg atcccctggt     2940

gctgatggtc ctgctggtgc tcctggtact cccgggcctc aaggtattgc tggacagcgt     3000

ggtgtggtcg gcctgcctgg tcagagagga gagagaggct tccctggtct tcctggcccc     3060

tctggtgaac ctggcaaaca aggtccctct ggagcaagtg gtgaacgtgg tccccctggt     3120

cccatgggcc cccctggatt ggctggaccc cctggtgaat ctggacgtga gggggctcct     3180

ggtgccgaag gttcccctgg acgagacggt tctcctggcg ccaagggtga ccgtggtgag     3240

accggccccg ctggaccccc tggtgctcct ggtgctcctg gtgcccctgg ccccgttggc     3300

cctgctggca agagtggtga tcgtggtgag actggtcctg ctggtcccgc cggtcctgtc     3360

ggccctgttg gcgcccgtgg ccccgccgga ccccaaggcc cccgtggtga caagggtgag     3420

acaggcgaac agggcgacag aggcataaag ggtcaccgtg gcttctctgg cctccagggt     3480

ccccctggcc ctcctggctc tcctggtgaa caaggtccct ctggagcctc tggtcctgct     3540

ggtccccgag gtccccctgg ctctgctggt gctcctggca aagatggact caacggtctc     3600

cctggcccca ttgggccccc tggtcctcgc ggtcgcactg gtgatgctgg tcctgttggt     3660

ccccccggcc ctcctggacc tcctggtccc cctggtcctc ccagcgctgg tttcgacttc     3720

agcttcctgc cccagccacc tcaagagaag gctcacgatg gtggccgcta ctaccgggct     3780

gatgatgcca atgtggttcg tgaccgtgac ctcgaggtgg acaccaccct caagagcctg     3840

agccagcaga tcgagaacat ccggagccca gagggcagcc gcaagaaccc cgcccgcacc     3900

tgccgtgacc tcaagatgtg ccactctgac tggaagagtg gagagtactg gattgacccc     3960

aaccaaggct gcaacctgga tgccatcaaa gtcttctgca acatggagac tggtgagacc     4020

tgcgtgtacc ccactcagcc cagtgtggcc cagaagaact ggtacatcag caagaacccc     4080

aaggacaaga ggcatgtctg gttcggcgag agcatgaccg atggattcca gttcgagtat     4140

ggcggccagg gctccgaccc tgccgatgtg gccatccagc tgaccttcct gcgcctgatg     4200

tccaccgagg cctcccagaa catcacctac cactgcaaga acagcgtggc ctacatggac     4260

cagcagactg gcaacctcaa gaaggccctg ctcctccagg gctccaacga gatcgagatc     4320

cgcgccgagg gcaacagccg cttcacctac agcgtcactg tcgatggctg cacgagtcac     4380

accggagcct ggggcaagac agtgattgaa tacaaaacca ccaagacctc ccgcctgccc     4440

atcatcgatg tggccccctt ggacgttggt gccccagacc aggaattcgg cttcgacgtt     4500

ggccctgtct gcttcctgta aactccctcc atcccaacct ggctccctcc cacccaacca     4560

actttccccc caacccggaa acagacaagc aacccaaact gaaccccctc aaaagccaaa     4620

aaatgggaga caatttcaca tggactttgg aaaatatttt tttcctttgc attcatctct     4680

caaacttagt ttttatcttt gaccaaccga acatgaccaa aaaccaaaag tgcattcaac     4740

cttaccaaaa aaaaaaaaaa aaaaagaata aataaataac tttttaaaaa aggaagcttg     4800

gtccacttgc ttgaagaccc atgcgggggt aagtcccttt ctgcccgttg ggcttatgaa     4860

accccaatgc tgccctttct gctcctttct ccacaccccc cttggggcct cccctccact     4920

ccttcccaaa tctgtctccc cagaagacac aggaaacaat gtattgtctg cccagcaatc     4980

aaaggcaatg ctcaaacacc caagtggccc ccaccctcag cccgctcctg cccgcccagc     5040

acccccaggc cctgggggac ctggggttct cagactgcca aagaagcctt gccatctggc     5100

gctcccatgg ctcttgcaac atctcccctt cgtttttgag ggggtcatgc cgggggagcc     5160

accagcccct cactgggttc ggaggagagt caggaagggc cacgacaaag cagaaacatc     5220

ggatttgggg aacgcgtgtc aatcccttgt gccgcagggc tgggcgggag agactgttct     5280

gttccttgtg taactgtgtt gctgaaagac tacctcgttc ttgtcttgat gtgtcaccgg     5340

ggcaactgcc tgggggcggg gatgggggca gggtggaagc ggctccccat tttataccaa     5400

aggtgctaca tctatgtgat gggtggggtg gggagggaat cactggtgct atagaaattg     5460

agatgccccc ccaggccagc aaatgttcct ttttgttcaa agtctatttt tattccttga     5520

tatttttctt tttttttttt tttttttgtg gatggggact tgtgaatttt tctaaaggtg     5580

ctatttaaca tgggaggaga gcgtgtgcgg ctccagccca gcccgctgct cactttccac     5640

cctctctcca cctgcctctg gcttctcagg cctctgctct ccgacctctc tcctctgaaa     5700

ccctcctcca cagctgcagc ccatcctccc ggctccctcc tagtctgtcc tgcgtcctct     5760

gtccccgggt ttcagagaca acttcccaaa gcacaaagca gtttttcccc ctaggggtgg     5820

gaggaagcaa aagactctgt acctattttg tatgtgtata ataatttgag atgtttttaa     5880

ttattttgat tgctggaata aagcatgtgg aaatgaccca aacataa                   5927


<210>  77
<211>  1203
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Activin A mRNA

<400>  77
atgcccttgc tttggctgag aggatttctg ttggcaagtt gctggattat agtgaggagt       60

tcccccaccc caggatccga ggggcacagc gcggcccccg actgtccgtc ctgtgcgctg      120

gccgccctcc caaaggatgt acccaactct cagccagaga tggtggaggc cgtcaagaag      180

cacattttaa acatgctgca cttgaagaag agacccgatg tcacccagcc ggtacccaag      240

gcggcgcttc tgaacgcgat cagaaagctt catgtgggca aagtcgggga gaacgggtat      300

gtggagatag aggatgacat tggaaggagg gcagaaatga atgaacttat ggagcagacc      360

tcggagatca tcacgtttgc cgagtcaggt tggtgctggc attggcaggg ggtggggagg      420

ggtggggggt gggagggtaa aatatatttc tttgacagtc ccaggaggaa cttcttttcc      480

cttcagctgg aaactgcctg ggaaggttat tagttattag gtgatggtag cggactagcc      540

gacggagggc aggcagggga gggggagagg actttacaga aaaggaattc tcggtcgagc      600

tctgcctgga gatgactggc ttacacttac taaacccagc gggtcacaca gagaggaagc      660

tcggggccaa tgttgagctg gaaggcagac tgtgaggggc tgccttgccc tgcctgtgaa      720

acagatctga gcagccggag gaaagccgcg gcattttcgg gtgctagggg agcagaggag      780

gcttccggac cccatccaag tttttattga gggtagaggg gtgaatgtac caggattgga      840

gtggaatggc acagatgaag tcactctctt aaaacaaacc ttccccttta aaagtccaat      900

ctggggccac attggagaag cagggcatat ttatgagtga cagtcatttt tacctttaga      960

aaatgtctat aagtgcacag gcaccacatt caagacaggg aagagctact ttgggggaca     1020

gttgtcattg aaccagcagt tacttttggg acactgactt ttgctctctg aaagaaaaaa     1080

aaataaataa aacaaccagt tttgttcttt ctaaagttac taagagctct ctgccaagga     1140

acgaaccttg acaaagtact ctcagatact acgctgaagt cactcaatct taagaggaag     1200

aag                                                                   1203


<210>  78
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Snai1 primer 1

<400>  78
tcggaagcct aactacagcg a                                                 21


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Snai1 primer 2

<400>  79
agatgagcat tggcagcgag                                                   20


<210>  80
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dkk1 primer 1

<400>  80
ccttgaactc ggttctcaat tcc                                               23


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dkk1 primer 2

<400>  81
caatggtctg gtacttattc ccg                                               23


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Col1a1 primer 1

<400>  82
gagggccaag acgaagacat c                                                 21


<210>  83
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Col1a1 primer 2

<400>  83
cagatcacgt catcgcacaa c                                                 21


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Activin primer 1

<400>  84
tcatcacgtt tgccgagtca                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Activin primer 2

<400>  85
gctagtccgc taccatcacc                                                   20


<210>  86
<211>  4241
<212>  DNA
<213>  Homo sapiens

<220>
<223>  Human Axin2 mRNA

<400>  86
actggctccg cgagcctggc ccgggggagt cggctggagc cggctgcgct ttgataaggt       60

cctggcaact cagtaacagc ccgagagccg ggaaataaaa ataacccctc agagcgatgg      120

atttcggggc cgcccggcgg ccgaggcgcc cgccgaaggc cctgctgtaa aagagaggag      180

gttcagatga gcccctgctg acttgagaga gacagagaga ccacgccgat tgctgagagg      240

aactggaaga agaaaaattc ccagactcag tgggaagagc tccctcacca tgagtagcgc      300

tatgttggtg acttgcctcc cggaccccag cagcagcttc cgtgaggatg ccccgcggcc      360

cccagtgcca ggggaagaag gggagacccc accgtgtcag ccaggggtgg gcaagggcca      420

ggtcaccaaa cccatgcctg tctcttccaa caccaggcgg aacgaagatg ggttggggga      480

gccggagggg cgggcatctc cggattcccc tctgacccgg tggaccaagt ccttacactc      540

cttattgggc gatcaagacg gtgcttacct gttccgaact ttcctggaga gggagaaatg      600

cgtggatacc ttagacttct ggtttgcctg caatggattc aggcagatga acctgaagga      660

taccaaaact ttacgagtag ccaaagcgat ctacaaaagg tacattgaga acaacagcat      720

tgtctccaag cagctgaagc ctgccaccaa gacctacata agagatggca tcaagaagca      780

gcagattgat tccatcatgt ttgaccaggc gcagaccgag atccagtcgg tgatggagga      840

aaatgcctac cagatgtttt tgacttctga tatatacctc gaatatgtga ggagtggggg      900

agaaaacaca gcttacatga gtaatggggg actcgggagc ctaaaggtcg tgtgtggcta      960

tctccccacc ttgaatgaag aagaggagtg gacttgtgcc gacttcaagt gcaaactttc     1020

gccaaccgtg gttggcttgt ccagcaaaac tctgagggcc acggcgagtg tgaggtccac     1080

ggaaactgtt gacagtggat acaggtcctt caagaggagc gatcctgtta atccttatca     1140

cataggttct ggctatgtct ttgcaccagc caccagcgcc aacgacagtg agatatccag     1200

tgatgcgctg acggatgatt ccatgtccat gacggacagc agtgtagatg gaattcctcc     1260

ttatcgtgtg ggcagtaaga aacagctcca gagagaaatg catcgcagtg tgaaggccaa     1320

tggccaagtg tctctacctc atttcccgag aacccaccgc ctgcccaagg agatgacccc     1380

cgtggaaccc gccacctttg cagctgagct gatctcgagg ctggaaaagc tgaagctgga     1440

gttggagagc cgccacagcc tggaggagcg cctgcagcag atccgagagg atgaagagag     1500

agagggctcc gagctcacac tcaattcgcg ggagggggcg cccacgcagc accccctctc     1560

cctactgccc tccggcagct acgaggaaga cccgcagacg atactggacg atcacctgtc     1620

cagggtcctc aagacccctg gctgccagtc tccaggcgta ggccgctata gcccccgctc     1680

ccgctccccg gaccaccacc accaccacca ttcgcagtac cactccctgc tcccgcccgg     1740

tggcaagctg cctcccgcgg ccgcctcgcc gggcgcctgc cccctcctcg ggggcaaagg     1800

ctttgtgacc aagcagacga cgaagcatgt ccaccaccac tacatccacc accatgccgt     1860

ccccaagacc aaggaggaga tcgaggcgga ggccacgcag cgggtgcact gcttctgccc     1920

tgggggcagc gagtattact gctactcgaa atgcaaaagc cactccaagg ctccggaaac     1980

catgcccagc gagcagtttg gcggcagcag aggcagtacc ttgcccaaac gcaatgggaa     2040

aggcacggag ccgggcctgg ccctgcccgc cagggaagga ggggcccccg gcggagctgg     2100

ggccctgcag cttccccggg aggaaggaga caggtcgcag gatgtctggc agtggatgct     2160

ggagagtgag cggcagagca agcccaagcc ccatagtgcc caaagcacaa aaaaggccta     2220

ccccttggag tctgcccgct cgtctccagg cgaacgagcc agccggcacc atctgtgggg     2280

gggcaacagc gggcaccccc gcaccacccc ccgtgcccac ctgttcaccc aggaccctgc     2340

gatgcctccc ctgaccccac ccaacacgct ggctcagctg gaggaggcct gtcgcaggct     2400

agctgaggtg tcgaagcccc caaagcagcg gtgctgtgtg gccagtcagc agagggacag     2460

gaatcattcg gccactgttc agacgggagc cacacccttc tccaatccaa gcctggctcc     2520

agaagatcac aaagagccaa agaaactggc aggtgtccac gcgctccagg ccagtgagtt     2580

ggttgtcact tactttttct gtggggaaga aattccatac cggaggatgc tgaaggctca     2640

gagcttgacc ctgggccact ttaaagagca gctcagcaaa aagggaaatt ataggtatta     2700

cttcaaaaaa gcaagcgatg agtttgcctg tggagcggtg tttgaggaga tctgggagga     2760

tgagacggtg ctcccgatgt atgaaggccg gattctgggc aaagtggagc ggatcgattg     2820

agccctgggg tctggctttg gtgaactgtt ggagcccgaa gctcttgtga actgtcttgg     2880

ctgtgagcaa ctgcgacaaa acattttgaa ggaaaattaa accaatgaag aagacaaagt     2940

ctaaggaaga atcggccagt gggccttcgg gagggcgggg ggaggttgat tttcatgatt     3000

catgagctgg gtactgactg agataagaaa agcctgaact atttattaaa aacatgacca     3060

ctcttggcta ttgaagatgc tgcctgtatt tgagagactg ccatacataa tatatgactt     3120

cctagggatc tgaaatccat aaactaagag aaactgtgta tagcttacct gaacaggaat     3180

ccttactgat atttatagaa cagttgattt cccccatccc cagtttatgg atatgctgct     3240

ttaaacttgg aagggggaga caggaagttt taattgttct gactaaactt aggagttgag     3300

ctaggagtgc gttcatggtt tcttcactaa cagaggaatt atgctttgca ctacgtccct     3360

ccaagtgaag acagactgtt ttagacagac tttttaaaat ggtgccctac cattgacaca     3420

tgcagaaatt ggtgcgtttt gttttttttt ttcctatgct gctctgtttt gtcttaaagg     3480

tcttgagggt tgaccatgtt gcgtcatcat caacattttg ggggttgtgt tggatgggat     3540

gatctgttgc agagggagag gcagggaacc ctgctccttc gggccccagg ttgatcctgt     3600

gactgaggct ccccctcatg tagcctcccc aggcccaggg ccctgaggcc tgctagaatc     3660

actgccgctg tgctttcgtg gaaatgacag ttccttgttt tttttgtttc tgtttttgtt     3720

ttacattagt cattggacca cagccattca ggaactaccc cctgccccac aaagaaatga     3780

acagttgtag ggagacccag cagcaccttt cctccacaca ccttcatttt gatgttcggg     3840

tttttgtgtt aagttaatct gtacattctg tttgccattg ttacttgtac tatacatctg     3900

tatatagtgt acggcaaaag agtattaatc cactatctct agtgcttgac tttaaatcag     3960

tacagtacct gtacctgcac ggtcacccgc tccgtgtgtc gccctatatt gagggctcaa     4020

gctttccctt gttttttgaa aggggtttat gtataaatat attttatgcc tttttattac     4080

aagtcttgta ctcaatgact tttgtcatga cattttgttc tacttatact gtaaattatg     4140

cattataaag agttcattta aggaaaatta cttggtacaa taattattgt aattaagaga     4200

tgtagccttt attaaaattt tatatttttc aaaaaaaaaa a                         4241


<210>  87
<211>  843
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human Axin2 amino acid sequence

<400>  87

Met Ser Ser Ala Met Leu Val Thr Cys Leu Pro Asp Pro Ser Ser Ser 
1               5                   10                  15      


Phe Arg Glu Asp Ala Pro Arg Pro Pro Val Pro Gly Glu Glu Gly Glu 
            20                  25                  30          


Thr Pro Pro Cys Gln Pro Gly Val Gly Lys Gly Gln Val Thr Lys Pro 
        35                  40                  45              


Met Pro Val Ser Ser Asn Thr Arg Arg Asn Glu Asp Gly Leu Gly Glu 
    50                  55                  60                  


Pro Glu Gly Arg Ala Ser Pro Asp Ser Pro Leu Thr Arg Trp Thr Lys 
65                  70                  75                  80  


Ser Leu His Ser Leu Leu Gly Asp Gln Asp Gly Ala Tyr Leu Phe Arg 
                85                  90                  95      


Thr Phe Leu Glu Arg Glu Lys Cys Val Asp Thr Leu Asp Phe Trp Phe 
            100                 105                 110         


Ala Cys Asn Gly Phe Arg Gln Met Asn Leu Lys Asp Thr Lys Thr Leu 
        115                 120                 125             


Arg Val Ala Lys Ala Ile Tyr Lys Arg Tyr Ile Glu Asn Asn Ser Ile 
    130                 135                 140                 


Val Ser Lys Gln Leu Lys Pro Ala Thr Lys Thr Tyr Ile Arg Asp Gly 
145                 150                 155                 160 


Ile Lys Lys Gln Gln Ile Asp Ser Ile Met Phe Asp Gln Ala Gln Thr 
                165                 170                 175     


Glu Ile Gln Ser Val Met Glu Glu Asn Ala Tyr Gln Met Phe Leu Thr 
            180                 185                 190         


Ser Asp Ile Tyr Leu Glu Tyr Val Arg Ser Gly Gly Glu Asn Thr Ala 
        195                 200                 205             


Tyr Met Ser Asn Gly Gly Leu Gly Ser Leu Lys Val Val Cys Gly Tyr 
    210                 215                 220                 


Leu Pro Thr Leu Asn Glu Glu Glu Glu Trp Thr Cys Ala Asp Phe Lys 
225                 230                 235                 240 


Cys Lys Leu Ser Pro Thr Val Val Gly Leu Ser Ser Lys Thr Leu Arg 
                245                 250                 255     


Ala Thr Ala Ser Val Arg Ser Thr Glu Thr Val Asp Ser Gly Tyr Arg 
            260                 265                 270         


Ser Phe Lys Arg Ser Asp Pro Val Asn Pro Tyr His Ile Gly Ser Gly 
        275                 280                 285             


Tyr Val Phe Ala Pro Ala Thr Ser Ala Asn Asp Ser Glu Ile Ser Ser 
    290                 295                 300                 


Asp Ala Leu Thr Asp Asp Ser Met Ser Met Thr Asp Ser Ser Val Asp 
305                 310                 315                 320 


Gly Ile Pro Pro Tyr Arg Val Gly Ser Lys Lys Gln Leu Gln Arg Glu 
                325                 330                 335     


Met His Arg Ser Val Lys Ala Asn Gly Gln Val Ser Leu Pro His Phe 
            340                 345                 350         


Pro Arg Thr His Arg Leu Pro Lys Glu Met Thr Pro Val Glu Pro Ala 
        355                 360                 365             


Thr Phe Ala Ala Glu Leu Ile Ser Arg Leu Glu Lys Leu Lys Leu Glu 
    370                 375                 380                 


Leu Glu Ser Arg His Ser Leu Glu Glu Arg Leu Gln Gln Ile Arg Glu 
385                 390                 395                 400 


Asp Glu Glu Arg Glu Gly Ser Glu Leu Thr Leu Asn Ser Arg Glu Gly 
                405                 410                 415     


Ala Pro Thr Gln His Pro Leu Ser Leu Leu Pro Ser Gly Ser Tyr Glu 
            420                 425                 430         


Glu Asp Pro Gln Thr Ile Leu Asp Asp His Leu Ser Arg Val Leu Lys 
        435                 440                 445             


Thr Pro Gly Cys Gln Ser Pro Gly Val Gly Arg Tyr Ser Pro Arg Ser 
    450                 455                 460                 


Arg Ser Pro Asp His His His His His His Ser Gln Tyr His Ser Leu 
465                 470                 475                 480 


Leu Pro Pro Gly Gly Lys Leu Pro Pro Ala Ala Ala Ser Pro Gly Ala 
                485                 490                 495     


Cys Pro Leu Leu Gly Gly Lys Gly Phe Val Thr Lys Gln Thr Thr Lys 
            500                 505                 510         


His Val His His His Tyr Ile His His His Ala Val Pro Lys Thr Lys 
        515                 520                 525             


Glu Glu Ile Glu Ala Glu Ala Thr Gln Arg Val His Cys Phe Cys Pro 
    530                 535                 540                 


Gly Gly Ser Glu Tyr Tyr Cys Tyr Ser Lys Cys Lys Ser His Ser Lys 
545                 550                 555                 560 


Ala Pro Glu Thr Met Pro Ser Glu Gln Phe Gly Gly Ser Arg Gly Ser 
                565                 570                 575     


Thr Leu Pro Lys Arg Asn Gly Lys Gly Thr Glu Pro Gly Leu Ala Leu 
            580                 585                 590         


Pro Ala Arg Glu Gly Gly Ala Pro Gly Gly Ala Gly Ala Leu Gln Leu 
        595                 600                 605             


Pro Arg Glu Glu Gly Asp Arg Ser Gln Asp Val Trp Gln Trp Met Leu 
    610                 615                 620                 


Glu Ser Glu Arg Gln Ser Lys Pro Lys Pro His Ser Ala Gln Ser Thr 
625                 630                 635                 640 


Lys Lys Ala Tyr Pro Leu Glu Ser Ala Arg Ser Ser Pro Gly Glu Arg 
                645                 650                 655     


Ala Ser Arg His His Leu Trp Gly Gly Asn Ser Gly His Pro Arg Thr 
            660                 665                 670         


Thr Pro Arg Ala His Leu Phe Thr Gln Asp Pro Ala Met Pro Pro Leu 
        675                 680                 685             


Thr Pro Pro Asn Thr Leu Ala Gln Leu Glu Glu Ala Cys Arg Arg Leu 
    690                 695                 700                 


Ala Glu Val Ser Lys Pro Pro Lys Gln Arg Cys Cys Val Ala Ser Gln 
705                 710                 715                 720 


Gln Arg Asp Arg Asn His Ser Ala Thr Val Gln Thr Gly Ala Thr Pro 
                725                 730                 735     


Phe Ser Asn Pro Ser Leu Ala Pro Glu Asp His Lys Glu Pro Lys Lys 
            740                 745                 750         


Leu Ala Gly Val His Ala Leu Gln Ala Ser Glu Leu Val Val Thr Tyr 
        755                 760                 765             


Phe Phe Cys Gly Glu Glu Ile Pro Tyr Arg Arg Met Leu Lys Ala Gln 
    770                 775                 780                 


Ser Leu Thr Leu Gly His Phe Lys Glu Gln Leu Ser Lys Lys Gly Asn 
785                 790                 795                 800 


Tyr Arg Tyr Tyr Phe Lys Lys Ala Ser Asp Glu Phe Ala Cys Gly Ala 
                805                 810                 815     


Val Phe Glu Glu Ile Trp Glu Asp Glu Thr Val Leu Pro Met Tyr Glu 
            820                 825                 830         


Gly Arg Ile Leu Gly Lys Val Glu Arg Ile Asp 
        835                 840             


