               SEQUENCE LISTING

<110> ACIB GmbH - Austrian Centre of Industrial Biotechnology

<120> Compact and optimized metabolic pathway design in pichia pastoris

<130> AC010P

<150> EP14190702.2
<151> 2014-10-28

<160> 99

<170> BiSSAP 1.3

<210> 1
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A1

<400> 1
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60


<210> 2
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A2

<400> 2
cgtgccgaag gacgtggatc ccttttgacc tgcggagatg tcgaagagaa tcctggacct     60


<210> 3
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A3

<400> 3
agagcagaag gtcgtggctc attgctgact tgtggcgacg tggaggaaaa tcccggacca     60


<210> 4
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A4

<400> 4
cgtgcagagg gccgtggttc cttacttacc tgcggtgatg tggaagaaaa tccaggaccc     60


<210> 5
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A5

<400> 5
cgtgccgagg gtaggggatc acttcttaca tgtggagacg tcgaggagaa ccctggtcca     60


<210> 6
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A6

<400> 6
agagctgaag gaaggggttc cctgttaacg tgtggcgatg ttgaagagaa ccccggtcct     60


<210> 7
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A7

<400> 7
agggcagaag gcagaggatc tctgttgact tgtggtgatg tagaggagaa tcccggccca     60


<210> 8
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A8

<400> 8
agggcggagg ggagaggctc tcttttaact tgtggagatg tggaagagaa cccaggccct     60


<210> 9
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> P2A

<400> 9
gctactaact tctctttgct taagcaagct ggtgacgttg aggaaaaccc aggtcca        57


<210> 10
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> FMDV2A

<400> 10
caattgctta acttcgactt attgaagctt gctggtgacg ttgagtctaa cccaggtcca     60


<210> 11
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> FMDV2A

<400> 11
Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 12
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A

<400> 12
Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 13
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> P2A

<400> 13
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      
Pro Gly Pro 
            

<210> 14
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> F2A

<400> 14
Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      
Asn Pro Gly Ala 
            20  

<210> 15
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_EcoRI_fwd

<400> 15
aaatgaattc cgaaacgatg gctagcaaag gagaagaact tttcactg                  48


<210> 16
<211> 92
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_FMDV2A_rev

<400> 16
tggacctggg ttagactcaa cgtcaccagc aagcttcaat aagtcgaagt taagcaattg     60

cttgtacaat tcatccatgc catgtgtaat cc                                   92


<210> 17
<211> 89
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_P2A_rev

<400> 17
tggacctggg ttttcctcaa cgtcaccagc ttgcttaagc aaagagaagt tagtagcctt     60

gtacaattca tccatgccat gtgtaatcc                                       89


<210> 18
<211> 92
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_T2A_rev

<400> 18
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

cttgtacaat tcatccatgc catgtgtaat cc                                   92


<210> 19
<211> 93
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_FMDV2A_fwd_mit Startcodon

<400> 19
caattgctta acttcgactt attgaagctt gctggtgacg ttgagtctaa cccaggtcca     60

atggtttcta agggtgagga agttatcaag gag                                  93


<210> 20
<211> 96
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_FMDV2A_fwd_ohne Startcodon

<400> 20
caattgctta acttcgactt attgaagctt gctggtgacg ttgagtctaa cccaggtcca     60

gtttctaagg gtgaggaagt tatcaaggag ttcatg                               96


<210> 21
<211> 93
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_P2A_fwd

<400> 21
gctactaact tctctttgct taagcaagct ggtgacgttg aggaaaaccc aggtccagtt     60

tctaagggtg aggaagttat caaggagttc atg                                  93


<210> 22
<211> 96
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_T2A_fwd

<400> 22
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

gtttctaagg gtgaggaagt tatcaaggag ttcatg                               96


<210> 23
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_NotI_rev

<400> 23
tattgcggcc gcttacttat aaagctcgtc cataccgtac aagaacaag                 49


<210> 24
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_EcoRI_fwd

<400> 24
aaatgaattc cgaaacgatg gtttctaagg gtgaggaagt tatcaaggag                50


<210> 25
<211> 94
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_FMDV2A_rev

<400> 25
tggacctggg ttagactcaa cgtcaccagc aagcttcaat aagtcgaagt taagcaattg     60

cttataaagc tcgtccatac cgtacaagaa caag                                 94


<210> 26
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_P2A_rev

<400> 26
tggacctggg ttttcctcaa cgtcaccagc ttgcttaagc aaagagaagt tagtagcctt     60

ataaagctcg tccataccgt acaagaacaa g                                    91


<210> 27
<211> 94
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_T2A_rev

<400> 27
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

cttataaagc tcgtccatac cgtacaagaa caag                                 94


<210> 28
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_ FMDV2A_fwd

<400> 28
caattgctta acttcgactt attgaagctt gctggtgacg ttgagtctaa cccaggtcca     60

gctagcaaag gagaagaact tttcactgga g                                    91


<210> 29
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_P2A_fwd

<400> 29
gctactaact tctctttgct taagcaagct ggtgacgttg aggaaaaccc aggtccagct     60

agcaaaggag aagaactttt cactggag                                        88


<210> 30
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_T2A_fwd

<400> 30
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

gctagcaaag gagaagaact tttcactgga g                                    91


<210> 31
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_NotI_rev

<400> 31
tattgcggcc gcttacttgt acaattcatc catgccatgt gtaatcc                   47


<210> 32
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_Gibson_rev

<400> 32
ctctcaggca aatggcattc tgacatcctc ttgagcggcc gcttacttat aaagctcgtc     60

cataccgtac aagaacaag                                                  79


<210> 33
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_Gibson_rev

<400> 33
ctctcaggca aatggcattc tgacatcctc ttgagcggcc gcttacttgt acaattcatc     60

catgccatgt gtaatcc                                                    77


<210> 34
<211> 103
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_AOX_Gibson_His_fwd

<400> 34
acgacaactt gagaagatca aaaaacaact aattattgaa agaattccga aacgatgcac     60

caccatcacc accatgctag caaaggagaa gaacttttca ctg                      103


<210> 35
<211> 105
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_AOX_Gibson_His_fwd

<400> 35
acgacaactt gagaagatca aaaaacaact aattattgaa agaattccga aacgatgcac     60

caccatcacc accatgtttc taagggtgag gaagttatca aggag                    105


<210> 36
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_GAP_Gibson_His_fwd

<400> 36
gtccctattt caatcaattg aacaactatc aaaacacaga attccgaaac gatgcaccac     60

catcaccacc atgctagcaa aggagaagaa cttttcactg                          100


<210> 37
<211> 102
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_GAP_Gibson_His_fwd

<400> 37
gtccctattt caatcaattg aacaactatc aaaacacaga attccgaaac gatgcaccac     60

catcaccacc atgtttctaa gggtgaggaa gttatcaagg ag                       102


<210> 38
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<223> pAOX1_crtE_fw

<400> 38
cgacaacttg agaagatcaa aaaacaacta attattgaaa gaattccgaa acgatgacgg     60

tctgc                                                                 65


<210> 39
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtE_T2A_rev

<400> 39
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

actgacggca gcgagttttt tgtc                                            84


<210> 40
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtB_T2A_fw

<400> 40
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

aataatccgt cgttactcaa tcatgcgg                                        88


<210> 41
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtB_T2A_rev

<400> 41
aggtccagga ttctcttcga catctccgca ggtcaaaagg gatccacgtc cttcggcacg     60

gagcgggcgc tgccagagat g                                               81


<210> 42
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtI_T2A_fw

<400> 42
cgtgccgaag gacgtggatc ccttttgacc tgcggagatg tcgaagagaa tcctggacct     60

aaaccaacta cggtaattgg tgcagg                                          86


<210> 43
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtI_T2A_rev

<400> 43
tggtccggga ttttcctcca cgtcgccaca agtcagcaat gagccacgac cttctgctct     60

tatcagatcc tccagcatca aacctgc                                         87


<210> 44
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_T2A_fw

<400> 44
agagcagaag gtcgtggctc attgctgact tgtggcgacg tggaggaaaa tcccggacca     60

caaccgcatt atgatctgat tctcgtgg                                        88


<210> 45
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_AOX_TT_rev

<400> 45
caggcaaatg gcattctgac atcctcttga gcggccgctt aacgatgagt cg             52


<210> 46
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGAP_crtE_fw

<400> 46
gtccctattt caatcaattg aacaactatc aaaacacaga attccgaaac gatgacggtc     60

tgc                                                                   63


<210> 47
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtB_FMDV2A_rev

<400> 47
tggacctggg ttagactcaa cgtcaccagc aagcttcaat aagtcgaagt taagcaattg     60

gagcgggcgc tgccagagat g                                               81


<210> 48
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtI_FMDV2A_fw

<400> 48
caattgctta acttcgactt attgaagctt gctggtgacg ttgagtctaa cccaggtcca     60

aaaccaacta cggtaattgg tgcagg                                          86


<210> 49
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtI_P2A_rev

<400> 49
tggacctggg ttttcctcaa cgtcaccagc ttgcttaagc aaagagaagt tagtagctat     60

cagatcctcc agcatcaaac ctgc                                            84


<210> 50
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_P2A_fw

<400> 50
gctactaact tctctttgct taagcaagct ggtgacgttg aggaaaaccc aggtccacaa     60

ccgcattatg atctgattct cgtgg                                           85


<210> 51
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGAP_vioC_fw

<400> 51
gtccctattt caatcaattg aacaactatc aaaacacaga attccgaaac gatgaagaga     60

gctatcattg                                                            70


<210> 52
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> pAOX1_vioC_fw

<400> 52
cgacaacttg agaagatcaa aaaacaacta attattgaaa gaattccgaa acgatgaaga     60

gagctatcat tg                                                         72


<210> 53
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioC_T2A4_rev

<400> 53
gggtcctgga ttttcttcca catcaccgca ggtaagtaag gaaccacggc cctctgcacg     60

gttaactcta ccaatcttgt accagacgtt c                                    91


<210> 54
<211> 91
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A4_vioB_fw

<400> 54
cgtgcagagg gccgtggttc cttacttacc tgcggtgatg tggaagaaaa tccaggaccc     60

tctattttgg acttcccaag aatccacttt c                                    91


<210> 55
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioB_T2A5_rev

<400> 55
tggaccaggg ttctcctcga cgtctccaca tgtaagaagt gatcccctac cctcggcacg     60

agcttcacga gataactttc cacaagc                                         87


<210> 56
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A5_vioE_fw

<400> 56
cgtgccgagg gtaggggatc acttcttaca tgtggagacg tcgaggagaa ccctggtcca     60

gaaaaccgtg agccaccttt gc                                              82


<210> 57
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioE_T2A6_rev

<400> 57
aggaccgggg ttctcttcaa catcgccaca cgttaacagg gaaccccttc cttcagctct     60

tctcttagcg gcgaagacag cg                                              82


<210> 58
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A6_vioD_fw

<400> 58
agagctgaag gaaggggttc cctgttaacg tgtggcgatg ttgaagagaa ccccggtcct     60

aagatccttg tgattggtgc aggac                                           85


<210> 59
<211> 89
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioD_T2A7_rev

<400> 59
tgggccggga ttctcctcta catcaccaca agtcaacagt gatcctctgc cttctgccct     60

tctttgcaag gcgtatctaa ggttttgtg                                       89


<210> 60
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A7_vioA_fw

<400> 60
agggcagaag gcagaggatc tctgttgact tgtggtgatg tagaggagaa tcccggccca     60

aaacactctt ccgacatttg tattgtcg                                        88


<210> 61
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioA_AOX_TT_rev

<400> 61
caggcaaatg gcattctgac atcctcttga gcggccgctt aggcagcaat tctttgcaaa     60

agcaaac                                                               67


<210> 62
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_T2A8_rev

<400> 62
agggcctggg ttctcttcca catctccaca agttaaaaga gagcctctcc cctccgccct     60

acgatgagtc gtcataatgg cttgc                                           85


<210> 63
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A8_ vioC_fw

<400> 63
agggcggagg ggagaggctc tcttttaact tgtggagatg tggaagagaa cccaggccct     60

aagagagcta tcattgttgg tggagg                                          86


<210> 64
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A_crtE_fw

<400> 64
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

gaattccgaa acgatgacgg tctgc                                           85


<210> 65
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtB_T2A_rev

<400> 65
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

gagcgggcgc tgccagagat g                                               81


<210> 66
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A_crtI_fw

<400> 66
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

aaaccaacta cggtaattgg tgcagg                                          86


<210> 67
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtI_T2A_rev

<400> 67
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

tatcagatcc tccagcatca aacctgc                                         87


<210> 68
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A_crtY_fw

<400> 68
agagctgagg gtagaggttc tttgcttact tgcggtgacg ttgaggaaaa cccaggtcca     60

caaccgcatt atgatctgat tctcgtgg                                        88


<210> 69
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_T2A_rev

<400> 69
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

gcggccgctt aacgatgagt cg                                              82


<210> 70
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGAP_vioA_fw

<400> 70
gtccctattt caatcaattg aacaactatc aaaacacaga attccgaaac gatgaaacac     60

tcttccgaca tttgtattgt cg                                              82


<210> 71
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> pAOX_vioA_fw

<400> 71
cgacaacttg agaagatcaa aaaacaacta attattgaaa gaattccgaa acgatgaaac     60

actcttccga catttgtatt gtcg                                            84


<210> 72
<211> 88
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioC_opt_T2A8_rev

<400> 72
agggcctggg ttctcttcca catctccaca agttaaaaga gagcctctcc cctccgccct     60

gttaactcta ccaatcttgt accagacg                                        88


<210> 73
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A8_crtE_fw

<400> 73
agggcggagg ggagaggctc tcttttaact tgtggagatg tggaagagaa cccaggccct     60

acggtctgcg caaaaaaaca cg                                              82


<210> 74
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> crtY_AOX_TT_rev

<400> 74
caggcaaatg gcattctgac atcctcttga gcggccgctt aacgatgagt cg             52


<210> 75
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAS1TT_vioA_fw

<400> 75
ctcctaacta aaactgtaaa gacttcccgt actagtttag gcagcaattc tttgcaaaag     60

caaacg                                                                66


<210> 76
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> HTX1_vioB_rev

<400> 76
gaaagtggat tcttgggaag tccaaaatag acattttgat ttgtttaggt aacttgaact     60

ggatgtatta gtttg                                                      75


<210> 77
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioB_TBF4_fw_new

<400> 77
gtggaaagtt atctcgtgaa gcttaagtac gtagtttcgc ttagtttaag actaaactaa     60

tgttg                                                                 65


<210> 78
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> TBF4_vioE_rev_new

<400> 78
cgctgtcttc gccgctaaga gataagccga atagtttgta tacgtcttat gtaatgagtt     60

tc                                                                    62


<210> 79
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioE_HHX1_fw

<400> 79
gcaaaggtgg ctcacggttt tccatttttc tttacctgga tataaataaa aaaaaggaaa     60

cacaatctct g                                                          71


<210> 80
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> HHX1_vioD_rev

<400> 80
cctgcaccaa tcacaaggat cttcatgttt tatcgatagt agttgagcaa taaaaaaaag     60

gagaaaaagc                                                            70


<210> 81
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioD_DAS2TT_fw

<400> 81
ccttagatac gccttgcaaa gataagtaga tttggccact aacgggttag tag            53


<210> 82
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAS2TT_GAP_rev

<400> 82
ggacaccaag acatttctac aaaaagacgg ggttcgtaaa ctggttcc                  48


<210> 83
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAS2TT_GAP_fw

<400> 83
gaggaaccag tttacgaacc ccgtcttttt gtagaaatgt cttggtgtcc tcgtcc         56


<210> 84
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> GAP_vioC_rev

<400> 84
caccaacaat gatagctctc ttcattgtgt tttgatagtt gttcaattga ttgaaatagg     60

gac                                                                   63


<210> 85
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> BZ6_vioB_rev

<400> 85
gattcttggg aagtccaaaa tagacatttt tgatgtttga tagtttgata agagtgaact     60

ttagtgttta g                                                          71


<210> 86
<211> 61
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioE_BZF8_fw

<400> 86
gcaaaggtgg ctcacggttt tccatcttag attttttttt ttgcttggtg ggattccttc     60

g                                                                     61


<210> 87
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> BZF8_vioD_rev

<400> 87
ctgcaccaat cacaaggatc ttcattgtga atatcaagaa ttgtatgaac aagcaaagtt     60

gg                                                                    62


<210> 88
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> DAS2TT_FDH1_fw

<400> 88
ggaaccagtt tacgaacccc gtctgggtgc ggaaccagct tctaattaaa tag            53


<210> 89
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> FDH1_vioC_rev

<400> 89
caccaacaat gatagctctc ttcattgttt aagtgggtga tgttggaggt atttg          55


<210> 90
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<223> vioB_TBF4_fw_new

<400> 90
gtggaaagtt atctcgtgaa gcttaagtac gtagtttcgc ttagtttaag actaaactaa     60

tgttg                                                                 65


<210> 91
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> TBF4_vioE_rev_new

<400> 91
cgctgtcttc gccgctaaga gataagccga atagtttgta tacgtcttat gtaatgagtt     60

tc                                                                    62


<210> 92
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A_Ubiqitin_GFP_fw

<400> 92
agagctgagg gtagaggttc tttgcttac                                      29


<210> 93
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A_Ubiqitin_GFP_rev

<400> 93
gggacaactc cagtgaaaag ttcttctcc                                      29


<210> 94
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ubiquitin_GFP_fw

<400> 94
gcacttggtc cttagactta gaggaggtat ggctagcaaa ggagaagaac ttttcactg      59


<210> 95
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ubiquitin_GFPohne_fw

<400> 95
gcacttggtc cttagactta gaggaggtgc tagcaaagga gaagaacttt tcactg         56


<210> 96
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ubiquitin_GFPohne_rev

<400> 96
cagtgaaaag ttcttctcct ttgctagcac ctcctctaag tctaaggacc aagtgc         56


<210> 97
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> eGFP_Gibson_rev

<400> 97
ctctcaggca aatggcattc tgacatcctc ttgagcggcc gcttacttgt acaattcatc     60

catgccatgt gtaatcc                                                    77


<210> 98
<211> 105
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_AOX_Gibson_His_fwd

<400> 98
acgacaactt gagaagatca aaaaacaact aattattgaa agaattccga aacgatgcac     60

caccatcacc accatgtttc taagggtgag gaagttatca aggag                    105


<210> 99
<211> 94
<212> DNA
<213> Artificial Sequence

<220> 
<223> sTomato_T2A_rev

<400> 99
tggacctggg ttttcctcaa cgtcaccgca agtaagcaaa gaacctctac cctcagctct     60

cttataaagc tcgtccatac cgtacaagaa caag                                 94


