                         SEQUENCE LISTING

<110>  Medizinische Universitt Graz
 
<120>  Generically encoded nitrogen monoxide sensors

<130>  M 10878-WO /DB.JvD

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  193
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ser Phe Ser Val Asp Val Leu Ala Asn Ile Ala Ile Glu Leu Gln 
1               5                   10                  15      


Arg Gly Ile Gly His Gln Asp Arg Phe Gln Arg Leu Ile Thr Thr Leu 
            20                  25                  30          


Arg Gln Val Leu Glu Cys Asp Ala Ser Ala Leu Leu Arg Tyr Asp Ser 
        35                  40                  45              


Arg Gln Phe Ile Pro Leu Ala Ile Asp Gly Leu Ala Lys Asp Val Leu 
    50                  55                  60                  


Gly Arg Arg Phe Ala Leu Glu Gly His Pro Arg Leu Glu Ala Ile Ala 
65                  70                  75                  80  


Arg Ala Gly Asp Val Val Arg Phe Pro Ala Asp Ser Glu Leu Pro Asp 
                85                  90                  95      


Pro Tyr Asp Gly Leu Ile Pro Gly Gln Glu Ser Leu Lys Val His Ala 
            100                 105                 110         


Cys Val Gly Leu Pro Leu Phe Ala Gly Gln Asn Leu Ile Gly Ala Leu 
        115                 120                 125             


Thr Leu Asp Gly Met Gln Pro Asp Gln Phe Asp Val Phe Ser Asp Glu 
    130                 135                 140                 


Glu Leu Arg Leu Ile Ala Ala Leu Ala Ala Gly Ala Leu Ser Asn Ala 
145                 150                 155                 160 


Leu Leu Ile Glu Gln Leu Glu Ser Gln Asn Met Leu Pro Gly Asp Ala 
                165                 170                 175     


Thr Pro Phe Glu Ala Val Lys Gln Thr Gln Met Ile Gly Leu Ser Pro 
            180                 185                 190         


Gly 
    


<210>  2
<211>  350
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Phe Ser Val Asp Val Leu Ala Asn Ile Ala Ile Glu Leu Gln 
1               5                   10                  15      


Arg Gly Ile Gly His Gln Asp Arg Phe Gln Arg Leu Ile Thr Thr Leu 
            20                  25                  30          


Arg Gln Val Leu Glu Cys Asp Ala Ser Ala Leu Leu Arg Tyr Asp Ser 
        35                  40                  45              


Arg Gln Phe Ile Pro Leu Ala Ile Asp Gly Leu Ala Lys Asp Val Leu 
    50                  55                  60                  


Gly Arg Arg Phe Ala Leu Glu Gly His Pro Arg Leu Glu Ala Ile Ala 
65                  70                  75                  80  


Arg Ala Gly Asp Val Val Arg Phe Pro Ala Asp Ser Glu Leu Pro Asp 
                85                  90                  95      


Pro Tyr Asp Gly Leu Ile Pro Gly Gln Glu Ser Leu Lys Val His Ala 
            100                 105                 110         


Cys Val Gly Leu Pro Leu Phe Ala Gly Gln Asn Leu Ile Gly Ala Leu 
        115                 120                 125             


Thr Leu Asp Gly Met Gln Pro Asp Gln Phe Asp Val Phe Ser Asp Glu 
    130                 135                 140                 


Glu Leu Arg Leu Ile Ala Ala Leu Ala Ala Gly Ala Leu Ser Asn Ala 
145                 150                 155                 160 


Leu Leu Ile Glu Gln Leu Glu Ser Gln Asn Met Leu Pro Gly Asp Ala 
                165                 170                 175     


Thr Pro Phe Glu Ala Val Lys Gln Thr Gln Met Ile Gly Leu Ser Pro 
            180                 185                 190         


Gly Met Thr Gln Leu Lys Lys Glu Ile Glu Ile Val Ala Ala Ser Asp 
        195                 200                 205             


Leu Asn Val Leu Ile Ser Gly Glu Thr Gly Thr Gly Lys Glu Leu Val 
    210                 215                 220                 


Ala Lys Ala Ile His Glu Ala Ser Pro Arg Ala Val Asn Pro Leu Val 
225                 230                 235                 240 


Tyr Leu Asn Cys Ala Ala Leu Pro Glu Ser Val Ala Glu Ser Glu Leu 
                245                 250                 255     


Phe Gly His Val Lys Gly Ala Phe Thr Gly Ala Ile Ser Asn Arg Ser 
            260                 265                 270         


Gly Lys Phe Glu Met Ala Asp Asn Gly Thr Leu Phe Leu Asp Glu Ile 
        275                 280                 285             


Gly Glu Leu Ser Leu Ala Leu Gln Ala Lys Leu Leu Arg Val Leu Gln 
    290                 295                 300                 


Tyr Gly Asp Ile Gln Arg Val Gly Asp Asp Arg Cys Leu Arg Val Asp 
305                 310                 315                 320 


Val Arg Val Leu Ala Ala Thr Asn Arg Asp Leu Arg Glu Glu Val Leu 
                325                 330                 335     


Ala Gly Arg Phe Arg Ala Asp Leu Phe His Arg Leu Ser Val 
            340                 345                 350 


<210>  3
<211>  504
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Ser Phe Ser Val Asp Val Leu Ala Asn Ile Ala Ile Glu Leu Gln 
1               5                   10                  15      


Arg Gly Ile Gly His Gln Asp Arg Phe Gln Arg Leu Ile Thr Thr Leu 
            20                  25                  30          


Arg Gln Val Leu Glu Cys Asp Ala Ser Ala Leu Leu Arg Tyr Asp Ser 
        35                  40                  45              


Arg Gln Phe Ile Pro Leu Ala Ile Asp Gly Leu Ala Lys Asp Val Leu 
    50                  55                  60                  


Gly Arg Arg Phe Ala Leu Glu Gly His Pro Arg Leu Glu Ala Ile Ala 
65                  70                  75                  80  


Arg Ala Gly Asp Val Val Arg Phe Pro Ala Asp Ser Glu Leu Pro Asp 
                85                  90                  95      


Pro Tyr Asp Gly Leu Ile Pro Gly Gln Glu Ser Leu Lys Val His Ala 
            100                 105                 110         


Cys Val Gly Leu Pro Leu Phe Ala Gly Gln Asn Leu Ile Gly Ala Leu 
        115                 120                 125             


Thr Leu Asp Gly Met Gln Pro Asp Gln Phe Asp Val Phe Ser Asp Glu 
    130                 135                 140                 


Glu Leu Arg Leu Ile Ala Ala Leu Ala Ala Gly Ala Leu Ser Asn Ala 
145                 150                 155                 160 


Leu Leu Ile Glu Gln Leu Glu Ser Gln Asn Met Leu Pro Gly Asp Ala 
                165                 170                 175     


Thr Pro Phe Glu Ala Val Lys Gln Thr Gln Met Ile Gly Leu Ser Pro 
            180                 185                 190         


Gly Met Thr Gln Leu Lys Lys Glu Ile Glu Ile Val Ala Ala Ser Asp 
        195                 200                 205             


Leu Asn Val Leu Ile Ser Gly Glu Thr Gly Thr Gly Lys Glu Leu Val 
    210                 215                 220                 


Ala Lys Ala Ile His Glu Ala Ser Pro Arg Ala Val Asn Pro Leu Val 
225                 230                 235                 240 


Tyr Leu Asn Cys Ala Ala Leu Pro Glu Ser Val Ala Glu Ser Glu Leu 
                245                 250                 255     


Phe Gly His Val Lys Gly Ala Phe Thr Gly Ala Ile Ser Asn Arg Ser 
            260                 265                 270         


Gly Lys Phe Glu Met Ala Asp Asn Gly Thr Leu Phe Leu Asp Glu Ile 
        275                 280                 285             


Gly Glu Leu Ser Leu Ala Leu Gln Ala Lys Leu Leu Arg Val Leu Gln 
    290                 295                 300                 


Tyr Gly Asp Ile Gln Arg Val Gly Asp Asp Arg Cys Leu Arg Val Asp 
305                 310                 315                 320 


Val Arg Val Leu Ala Ala Thr Asn Arg Asp Leu Arg Glu Glu Val Leu 
                325                 330                 335     


Ala Gly Arg Phe Arg Ala Asp Leu Phe His Arg Leu Ser Val Phe Pro 
            340                 345                 350         


Leu Ser Val Pro Pro Leu Arg Glu Arg Gly Asp Asp Val Ile Leu Leu 
        355                 360                 365             


Ala Gly Tyr Phe Cys Glu Gln Cys Arg Leu Arg Gln Gly Leu Ser Arg 
    370                 375                 380                 


Val Val Leu Ser Ala Gly Ala Arg Asn Leu Leu Gln His Tyr Ser Phe 
385                 390                 395                 400 


Pro Gly Asn Val Arg Glu Leu Glu His Ala Ile His Arg Ala Val Val 
                405                 410                 415     


Leu Ala Arg Ala Thr Arg Ser Gly Asp Glu Val Ile Leu Glu Ala Gln 
            420                 425                 430         


His Phe Ala Phe Pro Glu Val Thr Leu Pro Thr Pro Glu Val Ala Ala 
        435                 440                 445             


Val Pro Val Val Lys Gln Asn Leu Arg Glu Ala Thr Glu Ala Phe Gln 
    450                 455                 460                 


Arg Glu Thr Ile Arg Gln Ala Leu Ala Gln Asn His His Asn Trp Ala 
465                 470                 475                 480 


Ala Cys Ala Arg Met Leu Glu Thr Asp Val Ala Asn Leu His Arg Leu 
                485                 490                 495     


Ala Lys Arg Leu Gly Leu Lys Asp 
            500                 


<210>  4
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CFP

<400>  4

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Arg Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala His Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala 
225             


<210>  5
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YFP

<400>  5

Met Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
1               5                   10                  15      


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
            20                  25                  30          


Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
        35                  40                  45              


Leu Leu Glu Phe Val Thr Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
    50                  55                  60                  


Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe 
65                  70                  75                  80  


Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly 
                85                  90                  95      


His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly 
            100                 105                 110         


Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro 
        115                 120                 125             


Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala 
    130                 135                 140                 


Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met 
145                 150                 155                 160 


Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
                165                 170                 175     


Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val 
            180                 185                 190         


Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile 
        195                 200                 205             


Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile 
    210                 215                 220                 


Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
225                 230                 235                 240 


His Asn Ile Glu 
                


<210>  6
<211>  2014
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CFP-GAF-YFP

<400>  6
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccatcgat atgagttttt ccgttgatgt gctggcgaat      720

atcgccatcg aattgcagcg tgggattggt caccaggatc gttttcagcg cctgatcacc      780

acgctacgtc aggtgctgga gtgcgatgcg tctgcgttgc tacgttacga ttcgcggcag      840

tttattccgc ttgccatcga cggtctggca aaggatgtac tcggtagacg ctttgcgctg      900

gaagggcatc cacggctgga agcgattgcc cgcgccgggg atgtggtgcg ctttcccgca      960

gacagcgaat tgcccgatcc ctatgacggt ttgattcctg ggcaggagag tctgaaggtt     1020

cacgcctgcg ttggtctgcc attgtttgcc gggcaaaacc tgatcggcgc actgacgctc     1080

gacgggatgc agcccgatca gttcgatgtt ttcagcgacg aagagctacg gctgattgct     1140

gcgctggcgg cgggagcgtt aagcaatgcg ttgctgattg aacaactgga aagccagaat     1200

atgctgccag gcgatgccac gccgtttgaa gcggtgaaac agacgcagat gattggcttg     1260

tcccctggcg aattcatgga cggcggcgtg cagctcgccg accactacca gcagaacacc     1320

cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag     1380

ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc     1440

gggatcactc tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag     1500

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac     1560

ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc     1620

ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc     1680

ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc     1740

ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac     1800

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc     1860

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac     1920

aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc     1980

aacttcaaga tccgccacaa catcgagtaa gctt                                 2014


<210>  7
<211>  2485
<212>  DNA
<213>  artificial sequence

<220>
<223>  CFP-GAF-AAA-CFP

<400>  7
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccatcgat atgagttttt ccgttgatgt gctggcgaat      720

atcgccatcg aattgcagcg tgggattggt caccaggatc gttttcagcg cctgatcacc      780

acgctacgtc aggtgctgga gtgcgatgcg tctgcgttgc tacgttacga ttcgcggcag      840

tttattccgc ttgccatcga cggtctggca aaggatgtac tcggtagacg ctttgcgctg      900

gaagggcatc cacggctgga agcgattgcc cgcgccgggg atgtggtgcg ctttcccgca      960

gacagcgaat tgcccgatcc ctatgacggt ttgattcctg ggcaggagag tctgaaggtt     1020

cacgcctgcg ttggtctgcc attgtttgcc gggcaaaacc tgatcggcgc actgacgctc     1080

gacgggatgc agcccgatca gttcgatgtt ttcagcgacg aagagctacg gctgattgct     1140

gcgctggcgg cgggagcgtt aagcaatgcg ttgctgattg aacaactgga aagccagaat     1200

atgctgccag gcgatgccac gccgtttgaa gcggtgaaac agacgcagat gattggcttg     1260

tcccctggca tgacgcaact gaaaaaagag attgagattg tggcggcgtc cgatctcaac     1320

gtcctgatca gcggtgagac tggaaccggt aaggagctgg tggcgaaagc gattcatgaa     1380

gcctcgccac gggcggtgaa tccgctggtc tatctcaact gtgctgcact gccggaaagt     1440

gtggcggaaa gtgagttgtt cgggcatgtg aaaggagcgt ttactggcgc tatcagtaat     1500

cgcagcggga agtttgaaat ggcggataac ggcacgctgt ttctggatga gatcggcgag     1560

ttgtcgttgg cattgcaggc caagctgctg agggtgttgc agtatggcga tattcagcgc     1620

gttggcgatg accgttgttt gcgggtcgat gtgcgcgtgc tggcggcgac taaccgcgat     1680

ttacgcgaag aggtgctggc agggcgattc cgcgccgatt tgtttcatcg cctgagcgtg     1740

gaattcatgg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1800

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa     1860

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgggatcact     1920

ctcggcatgg acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag     1980

ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag     2040

ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg     2100

atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac     2160

ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc     2220

gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac     2280

aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag     2340

ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac     2400

agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag     2460

atccgccaca acatcgagta agctt                                           2485


<210>  8
<211>  2947
<212>  DNA
<213>  artificial sequence

<220>
<223>  CFP-GAF-AAA-HTH-YFP

<400>  8
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccatcgat atgagttttt ccgttgatgt gctggcgaat      720

atcgccatcg aattgcagcg tgggattggt caccaggatc gttttcagcg cctgatcacc      780

acgctacgtc aggtgctgga gtgcgatgcg tctgcgttgc tacgttacga ttcgcggcag      840

tttattccgc ttgccatcga cggtctggca aaggatgtac tcggtagacg ctttgcgctg      900

gaagggcatc cacggctgga agcgattgcc cgcgccgggg atgtggtgcg ctttcccgca      960

gacagcgaat tgcccgatcc ctatgacggt ttgattcctg ggcaggagag tctgaaggtt     1020

cacgcctgcg ttggtctgcc attgtttgcc gggcaaaacc tgatcggcgc actgacgctc     1080

gacgggatgc agcccgatca gttcgatgtt ttcagcgacg aagagctacg gctgattgct     1140

gcgctggcgg cgggagcgtt aagcaatgcg ttgctgattg aacaactgga aagccagaat     1200

atgctgccag gcgatgccac gccgtttgaa gcggtgaaac agacgcagat gattggcttg     1260

tcccctggca tgacgcaact gaaaaaagag attgagattg tggcggcgtc cgatctcaac     1320

gtcctgatca gcggtgagac tggaaccggt aaggagctgg tggcgaaagc gattcatgaa     1380

gcctcgccac gggcggtgaa tccgctggtc tatctcaact gtgctgcact gccggaaagt     1440

gtggcggaaa gtgagttgtt cgggcatgtg aaaggagcgt ttactggcgc tatcagtaat     1500

cgcagcggga agtttgaaat ggcggataac ggcacgctgt ttctggatga gatcggcgag     1560

ttgtcgttgg cattgcaggc caagctgctg agggtgttgc agtatggcga tattcagcgc     1620

gttggcgatg accgttgttt gcgggtcgat gtgcgcgtgc tggcggcgac taaccgcgat     1680

ttacgcgaag aggtgctggc agggcgattc cgcgccgatt tgtttcatcg cctgagcgtg     1740

tttccacttt cggtgccgcc gctgcgtgag cggggcgatg atgtcattct gctggcgggg     1800

tatttctgcg agcagtgtcg tttgcggcag gggctctccc gcgtggtatt aagtgccgga     1860

gcgcgaaatt tactgcaaca ctacagtttt ccgggaaacg tgcgcgaact ggaacatgct     1920

attcatcggg cggtagttct ggcgagagcc acccgcagcg gcgatgaagt gattcttgag     1980

gcgcaacatt ttgcttttcc tgaggtgacg ttgccgacgc cagaagtggc ggcggtgccc     2040

gttgttaagc aaaacctgcg tgaagcgaca gaagcgttcc agcgtgaaac tattcgtcag     2100

gcactggcac aaaatcatca caactgggct gcctgcgcgc ggatgctgga aaccgacgtc     2160

gccaacctgc atcggctggc gaaacgtctg ggattgaagg atgaattcat ggacggcggc     2220

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg     2280

cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc     2340

gatcacatgg tcctgctgga gttcgtgacc gccgggatca ctctcggcat ggacgagctg     2400

tacaagggtg gcagcggtgg catggtgagc aagggcgagg agctgttcac cggggtggtg     2460

cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag     2520

ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag     2580

ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc     2640

cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac     2700

gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg     2760

aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag     2820

gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc     2880

accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag     2940

taagctt                                                               2947


<210>  9
<211>  980
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CFP-GAF-AAA-HTH-cpV

<400>  9

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Arg Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala His Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Ile Asp Met Ser Phe Ser Val Asp Val Leu Ala Asn 
225                 230                 235                 240 


Ile Ala Ile Glu Leu Gln Arg Gly Ile Gly His Gln Asp Arg Phe Gln 
                245                 250                 255     


Arg Leu Ile Thr Thr Leu Arg Gln Val Leu Glu Cys Asp Ala Ser Ala 
            260                 265                 270         


Leu Leu Arg Tyr Asp Ser Arg Gln Phe Ile Pro Leu Ala Ile Asp Gly 
        275                 280                 285             


Leu Ala Lys Asp Val Leu Gly Arg Arg Phe Ala Leu Glu Gly His Pro 
    290                 295                 300                 


Arg Leu Glu Ala Ile Ala Arg Ala Gly Asp Val Val Arg Phe Pro Ala 
305                 310                 315                 320 


Asp Ser Glu Leu Pro Asp Pro Tyr Asp Gly Leu Ile Pro Gly Gln Glu 
                325                 330                 335     


Ser Leu Lys Val His Ala Cys Val Gly Leu Pro Leu Phe Ala Gly Gln 
            340                 345                 350         


Asn Leu Ile Gly Ala Leu Thr Leu Asp Gly Met Gln Pro Asp Gln Phe 
        355                 360                 365             


Asp Val Phe Ser Asp Glu Glu Leu Arg Leu Ile Ala Ala Leu Ala Ala 
    370                 375                 380                 


Gly Ala Leu Ser Asn Ala Leu Leu Ile Glu Gln Leu Glu Ser Gln Asn 
385                 390                 395                 400 


Met Leu Pro Gly Asp Ala Thr Pro Phe Glu Ala Val Lys Gln Thr Gln 
                405                 410                 415     


Met Ile Gly Leu Ser Pro Gly Met Thr Gln Leu Lys Lys Glu Ile Glu 
            420                 425                 430         


Ile Val Ala Ala Ser Asp Leu Asn Val Leu Ile Ser Gly Glu Thr Gly 
        435                 440                 445             


Thr Gly Lys Glu Leu Val Ala Lys Ala Ile His Glu Ala Ser Pro Arg 
    450                 455                 460                 


Ala Val Asn Pro Leu Val Tyr Leu Asn Cys Ala Ala Leu Pro Glu Ser 
465                 470                 475                 480 


Val Ala Glu Ser Glu Leu Phe Gly His Val Lys Gly Ala Phe Thr Gly 
                485                 490                 495     


Ala Ile Ser Asn Arg Ser Gly Lys Phe Glu Met Ala Asp Asn Gly Thr 
            500                 505                 510         


Leu Phe Leu Asp Glu Ile Gly Glu Leu Ser Leu Ala Leu Gln Ala Lys 
        515                 520                 525             


Leu Leu Arg Val Leu Gln Tyr Gly Asp Ile Gln Arg Val Gly Asp Asp 
    530                 535                 540                 


Arg Cys Leu Arg Val Asp Val Arg Val Leu Ala Ala Thr Asn Arg Asp 
545                 550                 555                 560 


Leu Arg Glu Glu Val Leu Ala Gly Arg Phe Arg Ala Asp Leu Phe His 
                565                 570                 575     


Arg Leu Ser Val Phe Pro Leu Ser Val Pro Pro Leu Arg Glu Arg Gly 
            580                 585                 590         


Asp Asp Val Ile Leu Leu Ala Gly Tyr Phe Cys Glu Gln Cys Arg Leu 
        595                 600                 605             


Arg Gln Gly Leu Ser Arg Val Val Leu Ser Ala Gly Ala Arg Asn Leu 
    610                 615                 620                 


Leu Gln His Tyr Ser Phe Pro Gly Asn Val Arg Glu Leu Glu His Ala 
625                 630                 635                 640 


Ile His Arg Ala Val Val Leu Ala Arg Ala Thr Arg Ser Gly Asp Glu 
                645                 650                 655     


Val Ile Leu Glu Ala Gln His Phe Ala Phe Pro Glu Val Thr Leu Pro 
            660                 665                 670         


Thr Pro Glu Val Ala Ala Val Pro Val Val Lys Gln Asn Leu Arg Glu 
        675                 680                 685             


Ala Thr Glu Ala Phe Gln Arg Glu Thr Ile Arg Gln Ala Leu Ala Gln 
    690                 695                 700                 


Asn His His Asn Trp Ala Ala Cys Ala Arg Met Leu Glu Thr Asp Val 
705                 710                 715                 720 


Ala Asn Leu His Arg Leu Ala Lys Arg Leu Gly Leu Lys Asp Glu Phe 
                725                 730                 735     


Met Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
            740                 745                 750         


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
        755                 760                 765             


Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
    770                 775                 780                 


Leu Leu Glu Phe Val Thr Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
785                 790                 795                 800 


Tyr Lys Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe 
                805                 810                 815     


Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly 
            820                 825                 830         


His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly 
        835                 840                 845             


Lys Leu Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro 
    850                 855                 860                 


Trp Pro Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala 
865                 870                 875                 880 


Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met 
                885                 890                 895     


Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly 
            900                 905                 910         


Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val 
        915                 920                 925             


Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile 
    930                 935                 940                 


Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile 
945                 950                 955                 960 


Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
                965                 970                 975     


His Asn Ile Glu 
            980 


<210>  10
<211>  669
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CFP-GAF-cpV

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Arg Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala His Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Ile Asp Met Ser Phe Ser Val Asp Val Leu Ala Asn 
225                 230                 235                 240 


Ile Ala Ile Glu Leu Gln Arg Gly Ile Gly His Gln Asp Arg Phe Gln 
                245                 250                 255     


Arg Leu Ile Thr Thr Leu Arg Gln Val Leu Glu Cys Asp Ala Ser Ala 
            260                 265                 270         


Leu Leu Arg Tyr Asp Ser Arg Gln Phe Ile Pro Leu Ala Ile Asp Gly 
        275                 280                 285             


Leu Ala Lys Asp Val Leu Gly Arg Arg Phe Ala Leu Glu Gly His Pro 
    290                 295                 300                 


Arg Leu Glu Ala Ile Ala Arg Ala Gly Asp Val Val Arg Phe Pro Ala 
305                 310                 315                 320 


Asp Ser Glu Leu Pro Asp Pro Tyr Asp Gly Leu Ile Pro Gly Gln Glu 
                325                 330                 335     


Ser Leu Lys Val His Ala Cys Val Gly Leu Pro Leu Phe Ala Gly Gln 
            340                 345                 350         


Asn Leu Ile Gly Ala Leu Thr Leu Asp Gly Met Gln Pro Asp Gln Phe 
        355                 360                 365             


Asp Val Phe Ser Asp Glu Glu Leu Arg Leu Ile Ala Ala Leu Ala Ala 
    370                 375                 380                 


Gly Ala Leu Ser Asn Ala Leu Leu Ile Glu Gln Leu Glu Ser Gln Asn 
385                 390                 395                 400 


Met Leu Pro Gly Asp Ala Thr Pro Phe Glu Ala Val Lys Gln Thr Gln 
                405                 410                 415     


Met Ile Gly Leu Ser Pro Gly Glu Phe Met Asp Gly Gly Val Gln Leu 
            420                 425                 430         


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
        435                 440                 445             


Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp 
    450                 455                 460                 


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
465                 470                 475                 480 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly 
                485                 490                 495     


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
            500                 505                 510         


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
        515                 520                 525             


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu Ile 
    530                 535                 540                 


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
545                 550                 555                 560 


Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
                565                 570                 575     


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
            580                 585                 590         


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
        595                 600                 605             


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
    610                 615                 620                 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
625                 630                 635                 640 


Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
                645                 650                 655     


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 
            660                 665                 


<210>  11
<211>  1205
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mKOk-GAF

<400>  11
ctcgagtatg gtgagtgtga ttaaaccaga gatgaagatg aggtactaca tggacggctc       60

cgtcaatggg catgagttca caattgaagg tgaaggcaca ggcagacctt acgagggaca      120

tcaagagatg acactacgcg tcacaatggc caagggcggg ccaatgcctt tcgcgtttga      180

cttagtgtca cacgtgttct gttacggcca cagacctttt actaaatatc cagaagagat      240

accagactat ttcaaacaag catttcctga aggcctgtca tgggaaaggt cgttggagtt      300

cgaagatggt gggtccgctt cagtcagtgc gcatataagc cttagaggaa acaccttcta      360

ccacaaatcc aaatttactg gggttaactt tcctgccgat ggtcctatca tgcaaaacca      420

aagtgttgat tgggagccat caaccgagaa aattactgcc agcgacggag ttctgaaggg      480

tgatgttacg atgtacctaa aacttgaagg aggcggcaat cacaaatgcc aattcaagac      540

tacttacaag gcggcaaaaa agattcttaa aatgccagga agccattaca tcagccatcg      600

cctcgtcagg aaaaccgaag gcaacattac tgagctggta gaagatgcag tagctcattc      660

catcgatatg agtttttccg ttgatgtgct ggcgaatatc gccatcgaat tgcagcgtgg      720

gattggtcac caggatcgtt ttcagcgcct gatcaccacg ctacgtcagg tgctggagtg      780

cgatgcgtct gcgttgctac gttacgattc gcggcagttt attccgcttg ccatcgacgg      840

tctggcaaag gatgtactcg gtagacgctt tgcgctggaa gggcatccac ggctggaagc      900

gattgcccgc gccggggatg tggtgcgctt tcccgcagac agcgaattgc ccgatcccta      960

tgacggtttg attcctgggc aggagagtct gaaggttcac gcctgcgttg gtctgccatt     1020

gtttgccggg caaaacctga tcggcgcact gacgctcgac gggatgcagc ccgatcagtt     1080

cgatgttttc agcgacgaag agctacggct gattgctgcg ctggcggcgg gagcgttaag     1140

caatgcgttg ctgattgaac aactggaaag ccagaatatg ctgccaggcg atgccacgta     1200

agctt                                                                 1205


<210>  12
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mKOk-GAF

<400>  12

Met Val Ser Val Ile Lys Pro Glu Met Lys Met Arg Tyr Tyr Met Asp 
1               5                   10                  15      


Gly Ser Val Asn Gly His Glu Phe Thr Ile Glu Gly Glu Gly Thr Gly 
            20                  25                  30          


Arg Pro Tyr Glu Gly His Gln Glu Met Thr Leu Arg Val Thr Met Ala 
        35                  40                  45              


Lys Gly Gly Pro Met Pro Phe Ala Phe Asp Leu Val Ser His Val Phe 
    50                  55                  60                  


Cys Tyr Gly His Arg Pro Phe Thr Lys Tyr Pro Glu Glu Ile Pro Asp 
65                  70                  75                  80  


Tyr Phe Lys Gln Ala Phe Pro Glu Gly Leu Ser Trp Glu Arg Ser Leu 
                85                  90                  95      


Glu Phe Glu Asp Gly Gly Ser Ala Ser Val Ser Ala His Ile Ser Leu 
            100                 105                 110         


Arg Gly Asn Thr Phe Tyr His Lys Ser Lys Phe Thr Gly Val Asn Phe 
        115                 120                 125             


Pro Ala Asp Gly Pro Ile Met Gln Asn Gln Ser Val Asp Trp Glu Pro 
    130                 135                 140                 


Ser Thr Glu Lys Ile Thr Ala Ser Asp Gly Val Leu Lys Gly Asp Val 
145                 150                 155                 160 


Thr Met Tyr Leu Lys Leu Glu Gly Gly Gly Asn His Lys Cys Gln Phe 
                165                 170                 175     


Lys Thr Thr Tyr Lys Ala Ala Lys Lys Ile Leu Lys Met Pro Gly Ser 
            180                 185                 190         


His Tyr Ile Ser His Arg Leu Val Arg Lys Thr Glu Gly Asn Ile Thr 
        195                 200                 205             


Glu Leu Val Glu Asp Ala Val Ala His Ser Ile Asp Met Ser Phe Ser 
    210                 215                 220                 


Val Asp Val Leu Ala Asn Ile Ala Ile Glu Leu Gln Arg Gly Ile Gly 
225                 230                 235                 240 


His Gln Asp Arg Phe Gln Arg Leu Ile Thr Thr Leu Arg Gln Val Leu 
                245                 250                 255     


Glu Cys Asp Ala Ser Ala Leu Leu Arg Tyr Asp Ser Arg Gln Phe Ile 
            260                 265                 270         


Pro Leu Ala Ile Asp Gly Leu Ala Lys Asp Val Leu Gly Arg Arg Phe 
        275                 280                 285             


Ala Leu Glu Gly His Pro Arg Leu Glu Ala Ile Ala Arg Ala Gly Asp 
    290                 295                 300                 


Val Val Arg Phe Pro Ala Asp Ser Glu Leu Pro Asp Pro Tyr Asp Gly 
305                 310                 315                 320 


Leu Ile Pro Gly Gln Glu Ser Leu Lys Val His Ala Cys Val Gly Leu 
                325                 330                 335     


Pro Leu Phe Ala Gly Gln Asn Leu Ile Gly Ala Leu Thr Leu Asp Gly 
            340                 345                 350         


Met Gln Pro Asp Gln Phe Asp Val Phe Ser Asp Glu Glu Leu Arg Leu 
        355                 360                 365             


Ile Ala Ala Leu Ala Ala Gly Ala Leu Ser Asn Ala Leu Leu Ile Glu 
    370                 375                 380                 


Gln Leu Glu Ser Gln Asn Met Leu Pro Gly Asp Ala Thr 
385                 390                 395         


