                         SEQUENCE LISTING

<110>  The Technical University of Denmark EP3670EP00
 
<120>  A two-cassette reporter system for assessing target gene 
       translation and target gene product inclusion body formation.

<130>  P3670EP00

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  295
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IbpAp

<400>  1
aattcatctg ttgatcgtgg gtgttggcct gatgagttat agcgatccct tgctgaaaat       60

aacatcatca ttacgtcgca ctgtggcggc tatcgcactt taacgtttcg tgctgccccc      120

tcagtctatg caatagacca taaactgcaa aaaaaagtcc gctgataagg cttgaaaagt      180

tcatttccag acccattttt acatcgtagc cgatgaggac gcgcctgatg ggtgttctgg      240

ctacctgacc tgtccattgt ggaaggtctt acattctcgc tgatttcagg agcta           295


<210>  2
<211>  756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-ASV

<400>  2
atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt       60

gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga      120

aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt      180

gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag      240

catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc      300

aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt      360

aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa      420

ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga      480

atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac      540

cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac      600

ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt      660

cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaaggcct      720

gcagcaaacg acgaaaacta cgctgcatca gtttaa                                756


<210>  3
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-mut3

<400>  3
atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt       60

gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga      120

aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt      180

gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag      240

catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc      300

aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt      360

aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa      420

ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga      480

atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac      540

cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac      600

ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt      660

cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataa         717


<210>  4
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  translation coupling cassette

<400>  4
actagtcatc atcaccacca tcattaggat ggtggtgatg ata                         43


<210>  5
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  5
atggttagca aaggtgaaga ggataatatg gccatcatca aagaatttat gcgctttaaa       60

gtgcacatgg aaggtagcgt taatggccat gaatttgaaa ttgaaggtga aggcgaaggt      120

cgtccgtatg aaggcaccca gaccgcaaaa ctgaaagtta ccaaaggtgg tccgctgccg      180

tttgcatggg atattctgag tccgcagttt atgtatggta gcaaagccta tgttaaacat      240

ccggcagata tcccggatta tctgaaactg agctttccgg aaggttttaa atgggaacgt      300

gtgatgaatt ttgaagatgg tggtgtggtg accgttaccc aggatagcag cctgcaggat      360

ggtgaattta tctataaagt taaactgcgt ggcaccaatt ttccgagtga tggtccggtt      420

atgcagaaaa aaacaatggg ttgggaagca agcagcgaac gtatgtatcc ggaagatggc      480

gcactgaaag gtgaaattaa acagcgcctg aaactgaaag atggtggcca ttatgatgca      540

gaagttaaaa ccacctataa agccaaaaaa ccggttcagc tgcctggtgc atataacgtt      600

aacattaaac tggatatcac cagccacaac gaggattata ccattgttga acagtatgaa      660

cgtgcagaag gtcgccatag taccggtggt atggatgaac tgtataaatg a               711


<210>  6
<211>  347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT

<400>  6
gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct gactctcggg       60

aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg ggggaagttg      120

acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt ggaaaagatg      180

aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga ggacttcctc      240

caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca catcttgtcc      300

ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggcccc                    347


<210>  7
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT

<400>  7
gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg       60

tacaagtttg ccagaaaaca ccacatcact ttaactaatc taattactga agagactact      120

catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta      180

ggaattgcgg gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa      240

agaaaaatgc tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac      300

caccaaggtc caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa      360

atctgttgct atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag      420

ctgtgtggtg cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac      480

ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg      540

cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt tggacagtgt agcactctac      600

cagtgccagg agctggacac ctacctgata ccccagatcc cc                         642


<210>  8
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT-STOP

<400>  8
gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg       60

tacaagtttg ccagaaaaca ccacatcact ttaactaatc taattactga agagactact      120

catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta      180

ggaattgcgg gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa      240

agaaaaatgc tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac      300

caccaaggtc caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa      360

atctgttgct atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag      420

ctgtgtggtg cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac      480

ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg      540

cagatgtgtg aggcacctgt ggtgacccga gagtgggtgt tggacagtgt agcactctac      600

cagtgccagg agctggacac ctacctgata ccccagatcc cctaa                      645


<210>  9
<211>  561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT-truncated

<400>  9
gtcaacaaaa gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg       60

tacaagtttg ccagaaaaca ccacatcact ttaactaatc taattactga agagactact      120

catgttgtta tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta      180

ggaattgcgg gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa      240

agaaaaatgc tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac      300

caccaaggtc caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa      360

atctgttgct atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag      420

ctgtgtggtg cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac      480

ccaattgtgg ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg      540

cagatgtgtg aggcacctgt g                                                561


<210>  10
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p19

<400>  10
ctgctggaag aagttcgcgc aggcgatcgt ctgagcggtg cagcagcacg tggtgatgtt       60

caagaagtgc gtcgtctgct gcatcgtgaa ctggttcatc ctgatgcact gaatcgtttt      120

ggtaaaaccg cactgcaggt tatgatgttt ggtagcaccg caattgcact ggaactgctg      180

aaacagggtg caagcccgaa tgttcaggat accagcggca ccagtccggt tcatgatgcc      240

gcacgtaccg gttttctgga taccctgaaa gttctggttg aacatggtgc agatgttaat      300

gttccggatg gtacaggtgc actgccgatt catctggccg tgcaagaagg tcataccgca      360

gttgttagct ttctggcagc agaaagcgat ctgcatcgtc gtgatgcacg tggtctgaca      420

ccgctggaac tggcactgca gcgtggtgca caggatctgg ttgatattct gcagggtcac      480

atggttgcac cgctg                                                       495


<210>  11
<211>  471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E6

<400>  11
gcgcgctttg aggatccaac acggcgaccc tacaagctac ctgatctgtg cacggaactg       60

aacacttcac tgcaagacat agaaataacc tgtgtatatt gcaagacagt attggaactt      120

acagaggtat ttgaatttgc atttaaagat ttatttgtgg tgtatagaga cagtataccg      180

catgctgcat gccataaatg tatagatttt tattctagaa ttagagaatt aagacattat      240

tcagactctg tgtatggaga cacattggaa aaactaacta acactgggtt atacaattta      300

ttaataaggt gcctgcggtg ccagaaaccg ttgaatccag cagaaaaact tagacacctt      360

aatgaaaaac gacgattcca caacatagct gggcactata gaggccagtg ccattcgtgc      420

tgcaaccgag cacgacagga aagactccaa cgacgcagag aaacacaagt a               471


<210>  12
<211>  1500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NusA

<400>  12
aacaaagaaa ttttggctgt agttgaagcc gtatccaatg aaaaggcgct acctcgcgag       60

aagattttcg aagcattgga aagcgcgctg gcgacagcaa caaagaaaaa atatgaacaa      120

gagatcgacg tccgcgtaca gatcgatcgc aaaagcggtg attttgacac tttccgtcgc      180

tggttagttg ttgatgaagt cacccagccg accaaggaaa tcacccttga agccgcacgt      240

tatgaagatg aaagcctgaa cctgggcgat tacgttgaag atcagattga gtctgttacc      300

tttgaccgta tcactaccca gacggcaaaa caggttatcg tgcagaaagt gcgtgaagcc      360

gaacgtgcga tggtggttga tcagttccgt gaacacgaag gtgaaatcat caccggcgtg      420

gtgaaaaaag taaaccgcga caacatctct ctggatctgg gcaacaacgc tgaagccgtg      480

atcctgcgcg aagatatgct gccgcgtgaa aacttccgcc ctggcgaccg cgttcgtggc      540

gtgctctatt ccgttcgccc ggaagcgcgt ggcgcgcaac tgttcgtcac tcgttccaag      600

ccggaaatgc tgatcgaact gttccgtatt gaagtgccag aaatcggcga agaagtgatt      660

gaaattaaag cagcggctcg cgatccgggt tctcgtgcga aaatcgcggt gaaaaccaac      720

gataaacgta tcgatccggt aggtgcttgc gtaggtatgc gtggcgcgcg tgttcaggcg      780

gtgtctactg aactgggtgg cgagcgtatc gatatcgtcc tgtgggatga taacccggcg      840

cagttcgtga ttaacgcaat ggcaccggca gacgttgctt ctatcgtggt ggatgaagat      900

aaacacacca tggatatcgc cgttgaagcc ggtaacctgg cgcaggcgat tggccgtaac      960

ggtcagaacg tgcgtctggc ttcgcagctg agcggttggg aactcaacgt gatgaccgtt     1020

gacgacctgc aggctaagca tcaggcggaa gcgcacgcag cgatcgacac cttcaccaaa     1080

tatctcgaca tcgacgaaga cttcgcgact gttctggtag aagaaggctt ctcgacgctg     1140

gaagaattgg cctatgtgcc gatgaaagag ctgttggaaa tcgaaggcct tgatgagccg     1200

accgttgaag cactgcgcga gcgtgctaaa aatgcactgg ccaccattgc acaggcccag     1260

gaagaaagcc tcggtgataa caaaccggct gacgatctgc tgaaccttga aggggtagat     1320

cgtgatttgg cattcaaact ggccgcccgt ggcgtttgta cgctggaaga tctcgccgaa     1380

cagggcattg atgatctggc tgatatcgaa gggttgaccg acgaaaaagc cggagcactg     1440

attatggctg cccgtaatat ttgctggttc ggtgacgaag cgggtagcgg ctctggtagc     1500


<210>  13
<211>  309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SUMO

<400>  13
tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag       60

actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag      120

accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac      180

tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg      240

gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg tggtagcggc      300

tctggtagc                                                              309


<210>  14
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP-ASV

<400>  14

Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Arg Pro 
225                 230                 235                 240 


Ala Ala Asn Asp Glu Asn Tyr Ala Ala Ser Val 
                245                 250     


<210>  15
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  translation coupling cassette

<400>  15

Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  16
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  16

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  17
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT

<400>  17

Val Asn Ser Ser Ala Ser Ala Asp Lys Pro Leu Ser Asn Met Lys Ile 
1               5                   10                  15      


Leu Thr Leu Gly Lys Leu Ser Arg Asn Lys Asp Glu Val Lys Ala Met 
            20                  25                  30          


Ile Glu Lys Leu Gly Gly Lys Leu Thr Gly Thr Ala Asn Lys Ala Ser 
        35                  40                  45              


Leu Cys Ile Ser Thr Lys Lys Glu Val Glu Lys Met Asn Lys Lys Met 
    50                  55                  60                  


Glu Glu Val Lys Glu Ala Asn Ile Arg Val Val Ser Glu Asp Phe Leu 
65                  70                  75                  80  


Gln Asp Val Ser Ala Ser Thr Lys Ser Leu Gln Glu Leu Phe Leu Ala 
                85                  90                  95      


His Ile Leu Ser Pro Trp Gly Ala Glu Val Lys Ala Glu Pro Val Glu 
            100                 105                 110         


Val Val Ala Pro 
        115     


<210>  18
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT

<400>  18

Val Asn Lys Arg Met Ser Met Val Val Ser Gly Leu Thr Pro Glu Glu 
1               5                   10                  15      


Phe Met Leu Val Tyr Lys Phe Ala Arg Lys His His Ile Thr Leu Thr 
            20                  25                  30          


Asn Leu Ile Thr Glu Glu Thr Thr His Val Val Met Lys Thr Asp Ala 
        35                  40                  45              


Glu Phe Val Cys Glu Arg Thr Leu Lys Tyr Phe Leu Gly Ile Ala Gly 
    50                  55                  60                  


Gly Lys Trp Val Val Ser Tyr Phe Trp Val Thr Gln Ser Ile Lys Glu 
65                  70                  75                  80  


Arg Lys Met Leu Asn Glu His Asp Phe Glu Val Arg Gly Asp Val Val 
                85                  90                  95      


Asn Gly Arg Asn His Gln Gly Pro Lys Arg Ala Arg Glu Ser Gln Asp 
            100                 105                 110         


Arg Lys Ile Phe Arg Gly Leu Glu Ile Cys Cys Tyr Gly Pro Phe Thr 
        115                 120                 125             


Asn Met Pro Thr Asp Gln Leu Glu Trp Met Val Gln Leu Cys Gly Ala 
    130                 135                 140                 


Ser Val Val Lys Glu Leu Ser Ser Phe Thr Leu Gly Thr Gly Val His 
145                 150                 155                 160 


Pro Ile Val Val Val Gln Pro Asp Ala Trp Thr Glu Asp Asn Gly Phe 
                165                 170                 175     


His Ala Ile Gly Gln Met Cys Glu Ala Pro Val Val Thr Arg Glu Trp 
            180                 185                 190         


Val Leu Asp Ser Val Ala Leu Tyr Gln Cys Gln Glu Leu Asp Thr Tyr 
        195                 200                 205             


Leu Ile Pro Gln Ile Pro 
    210                 


<210>  19
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT-STOP

<400>  19

Val Asn Lys Arg Met Ser Met Val Val Ser Gly Leu Thr Pro Glu Glu 
1               5                   10                  15      


Phe Met Leu Val Tyr Lys Phe Ala Arg Lys His His Ile Thr Leu Thr 
            20                  25                  30          


Asn Leu Ile Thr Glu Glu Thr Thr His Val Val Met Lys Thr Asp Ala 
        35                  40                  45              


Glu Phe Val Cys Glu Arg Thr Leu Lys Tyr Phe Leu Gly Ile Ala Gly 
    50                  55                  60                  


Gly Lys Trp Val Val Ser Tyr Phe Trp Val Thr Gln Ser Ile Lys Glu 
65                  70                  75                  80  


Arg Lys Met Leu Asn Glu His Asp Phe Glu Val Arg Gly Asp Val Val 
                85                  90                  95      


Asn Gly Arg Asn His Gln Gly Pro Lys Arg Ala Arg Glu Ser Gln Asp 
            100                 105                 110         


Arg Lys Ile Phe Arg Gly Leu Glu Ile Cys Cys Tyr Gly Pro Phe Thr 
        115                 120                 125             


Asn Met Pro Thr Asp Gln Leu Glu Trp Met Val Gln Leu Cys Gly Ala 
    130                 135                 140                 


Ser Val Val Lys Glu Leu Ser Ser Phe Thr Leu Gly Thr Gly Val His 
145                 150                 155                 160 


Pro Ile Val Val Val Gln Pro Asp Ala Trp Thr Glu Asp Asn Gly Phe 
                165                 170                 175     


His Ala Ile Gly Gln Met Cys Glu Ala Pro Val Val Thr Arg Glu Trp 
            180                 185                 190         


Val Leu Asp Ser Val Ala Leu Tyr Gln Cys Gln Glu Leu Asp Thr Tyr 
        195                 200                 205             


Leu Ile Pro Gln Ile Pro 
    210                 


<210>  20
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT-truncated

<400>  20

Val Asn Lys Arg Met Ser Met Val Val Ser Gly Leu Thr Pro Glu Glu 
1               5                   10                  15      


Phe Met Leu Val Tyr Lys Phe Ala Arg Lys His His Ile Thr Leu Thr 
            20                  25                  30          


Asn Leu Ile Thr Glu Glu Thr Thr His Val Val Met Lys Thr Asp Ala 
        35                  40                  45              


Glu Phe Val Cys Glu Arg Thr Leu Lys Tyr Phe Leu Gly Ile Ala Gly 
    50                  55                  60                  


Gly Lys Trp Val Val Ser Tyr Phe Trp Val Thr Gln Ser Ile Lys Glu 
65                  70                  75                  80  


Arg Lys Met Leu Asn Glu His Asp Phe Glu Val Arg Gly Asp Val Val 
                85                  90                  95      


Asn Gly Arg Asn His Gln Gly Pro Lys Arg Ala Arg Glu Ser Gln Asp 
            100                 105                 110         


Arg Lys Ile Phe Arg Gly Leu Glu Ile Cys Cys Tyr Gly Pro Phe Thr 
        115                 120                 125             


Asn Met Pro Thr Asp Gln Leu Glu Trp Met Val Gln Leu Cys Gly Ala 
    130                 135                 140                 


Ser Val Val Lys Glu Leu Ser Ser Phe Thr Leu Gly Thr Gly Val His 
145                 150                 155                 160 


Pro Ile Val Val Val Gln Pro Asp Ala Trp Thr Glu Asp Asn Gly Phe 
                165                 170                 175     


His Ala Ile Gly Gln Met Cys Glu Ala Pro Val 
            180                 185         


<210>  21
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  p19

<400>  21

Leu Leu Glu Glu Val Arg Ala Gly Asp Arg Leu Ser Gly Ala Ala Ala 
1               5                   10                  15      


Arg Gly Asp Val Gln Glu Val Arg Arg Leu Leu His Arg Glu Leu Val 
            20                  25                  30          


His Pro Asp Ala Leu Asn Arg Phe Gly Lys Thr Ala Leu Gln Val Met 
        35                  40                  45              


Met Phe Gly Ser Thr Ala Ile Ala Leu Glu Leu Leu Lys Gln Gly Ala 
    50                  55                  60                  


Ser Pro Asn Val Gln Asp Thr Ser Gly Thr Ser Pro Val His Asp Ala 
65                  70                  75                  80  


Ala Arg Thr Gly Phe Leu Asp Thr Leu Lys Val Leu Val Glu His Gly 
                85                  90                  95      


Ala Asp Val Asn Val Pro Asp Gly Thr Gly Ala Leu Pro Ile His Leu 
            100                 105                 110         


Ala Val Gln Glu Gly His Thr Ala Val Val Ser Phe Leu Ala Ala Glu 
        115                 120                 125             


Ser Asp Leu His Arg Arg Asp Ala Arg Gly Leu Thr Pro Leu Glu Leu 
    130                 135                 140                 


Ala Leu Gln Arg Gly Ala Gln Asp Leu Val Asp Ile Leu Gln Gly His 
145                 150                 155                 160 


Met Val Ala Pro Leu 
                165 


<210>  22
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E6

<400>  22

Ala Arg Phe Glu Asp Pro Thr Arg Arg Pro Tyr Lys Leu Pro Asp Leu 
1               5                   10                  15      


Cys Thr Glu Leu Asn Thr Ser Leu Gln Asp Ile Glu Ile Thr Cys Val 
            20                  25                  30          


Tyr Cys Lys Thr Val Leu Glu Leu Thr Glu Val Phe Glu Phe Ala Phe 
        35                  40                  45              


Lys Asp Leu Phe Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala Cys 
    50                  55                  60                  


His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His Tyr 
65                  70                  75                  80  


Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr Gly 
                85                  90                  95      


Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Cys Gln Lys Pro Leu Asn 
            100                 105                 110         


Pro Ala Glu Lys Leu Arg His Leu Asn Glu Lys Arg Arg Phe His Asn 
        115                 120                 125             


Ile Ala Gly His Tyr Arg Gly Gln Cys His Ser Cys Cys Asn Arg Ala 
    130                 135                 140                 


Arg Gln Glu Arg Leu Gln Arg Arg Arg Glu Thr Gln Val 
145                 150                 155         


<210>  23
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NusA

<400>  23

Asn Lys Glu Ile Leu Ala Val Val Glu Ala Val Ser Asn Glu Lys Ala 
1               5                   10                  15      


Leu Pro Arg Glu Lys Ile Phe Glu Ala Leu Glu Ser Ala Leu Ala Thr 
            20                  25                  30          


Ala Thr Lys Lys Lys Tyr Glu Gln Glu Ile Asp Val Arg Val Gln Ile 
        35                  40                  45              


Asp Arg Lys Ser Gly Asp Phe Asp Thr Phe Arg Arg Trp Leu Val Val 
    50                  55                  60                  


Asp Glu Val Thr Gln Pro Thr Lys Glu Ile Thr Leu Glu Ala Ala Arg 
65                  70                  75                  80  


Tyr Glu Asp Glu Ser Leu Asn Leu Gly Asp Tyr Val Glu Asp Gln Ile 
                85                  90                  95      


Glu Ser Val Thr Phe Asp Arg Ile Thr Thr Gln Thr Ala Lys Gln Val 
            100                 105                 110         


Ile Val Gln Lys Val Arg Glu Ala Glu Arg Ala Met Val Val Asp Gln 
        115                 120                 125             


Phe Arg Glu His Glu Gly Glu Ile Ile Thr Gly Val Val Lys Lys Val 
    130                 135                 140                 


Asn Arg Asp Asn Ile Ser Leu Asp Leu Gly Asn Asn Ala Glu Ala Val 
145                 150                 155                 160 


Ile Leu Arg Glu Asp Met Leu Pro Arg Glu Asn Phe Arg Pro Gly Asp 
                165                 170                 175     


Arg Val Arg Gly Val Leu Tyr Ser Val Arg Pro Glu Ala Arg Gly Ala 
            180                 185                 190         


Gln Leu Phe Val Thr Arg Ser Lys Pro Glu Met Leu Ile Glu Leu Phe 
        195                 200                 205             


Arg Ile Glu Val Pro Glu Ile Gly Glu Glu Val Ile Glu Ile Lys Ala 
    210                 215                 220                 


Ala Ala Arg Asp Pro Gly Ser Arg Ala Lys Ile Ala Val Lys Thr Asn 
225                 230                 235                 240 


Asp Lys Arg Ile Asp Pro Val Gly Ala Cys Val Gly Met Arg Gly Ala 
                245                 250                 255     


Arg Val Gln Ala Val Ser Thr Glu Leu Gly Gly Glu Arg Ile Asp Ile 
            260                 265                 270         


Val Leu Trp Asp Asp Asn Pro Ala Gln Phe Val Ile Asn Ala Met Ala 
        275                 280                 285             


Pro Ala Asp Val Ala Ser Ile Val Val Asp Glu Asp Lys His Thr Met 
    290                 295                 300                 


Asp Ile Ala Val Glu Ala Gly Asn Leu Ala Gln Ala Ile Gly Arg Asn 
305                 310                 315                 320 


Gly Gln Asn Val Arg Leu Ala Ser Gln Leu Ser Gly Trp Glu Leu Asn 
                325                 330                 335     


Val Met Thr Val Asp Asp Leu Gln Ala Lys His Gln Ala Glu Ala His 
            340                 345                 350         


Ala Ala Ile Asp Thr Phe Thr Lys Tyr Leu Asp Ile Asp Glu Asp Phe 
        355                 360                 365             


Ala Thr Val Leu Val Glu Glu Gly Phe Ser Thr Leu Glu Glu Leu Ala 
    370                 375                 380                 


Tyr Val Pro Met Lys Glu Leu Leu Glu Ile Glu Gly Leu Asp Glu Pro 
385                 390                 395                 400 


Thr Val Glu Ala Leu Arg Glu Arg Ala Lys Asn Ala Leu Ala Thr Ile 
                405                 410                 415     


Ala Gln Ala Gln Glu Glu Ser Leu Gly Asp Asn Lys Pro Ala Asp Asp 
            420                 425                 430         


Leu Leu Asn Leu Glu Gly Val Asp Arg Asp Leu Ala Phe Lys Leu Ala 
        435                 440                 445             


Ala Arg Gly Val Cys Thr Leu Glu Asp Leu Ala Glu Gln Gly Ile Asp 
    450                 455                 460                 


Asp Leu Ala Asp Ile Glu Gly Leu Thr Asp Glu Lys Ala Gly Ala Leu 
465                 470                 475                 480 


Ile Met Ala Ala Arg Asn Ile Cys Trp Phe Gly Asp Glu Ala Gly Ser 
                485                 490                 495     


Gly Ser Gly Ser 
            500 


<210>  24
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SUMO

<400>  24

Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu 
1               5                   10                  15      


Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser 
            20                  25                  30          


Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met 
        35                  40                  45              


Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe 
    50                  55                  60                  


Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp Leu 
65                  70                  75                  80  


Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly 
                85                  90                  95      


Gly Gly Ser Gly Ser Gly Ser 
            100             


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-fwd

<400>  25
agtatctaga atgcgtaaag gagaagaact t                                      31


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-ASV-rev

<400>  26
actgactagt ttaaactgat gcagcgtagt                                        30


<210>  27
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-mut3-rev

<400>  27
actgactagt ttatttgtat agttcatcca tgcc                                   34


<210>  28
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IbpAp-fwd

<400>  28
gagcttaatt aaaattcatc tgttgatcgt gg                                     32


<210>  29
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IbpAp-rev

<400>  29
gatatctaga tagctcctga aatcagcgag aatgtaag                               38


<210>  30
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry-fwd

<400>  30
tcaccaccat cattaggatg gtggtgatga taatggttag caaaggtgaa gagga            55


<210>  31
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry-rev

<400>  31
ggtgctcgag tgcggccgca agctttcatt tatacagttc atcca                       45


<210>  32
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA-BRCT-fwd

<400>  32
actttaagaa ggagatatac atatggtcaa caaaagaatg tccatggtg                   49


<210>  33
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA-BRCT-rev-1

<400>  33
caccatccta atgatggtgg tgatgatgac tagtggggat ctggggtatc aggta            55


<210>  34
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA-BRCT-rev-2

<400>  34
caccatccta atgatggtgg tgatgatgac tagtcacagg tgcctcacac atct             54


<210>  35
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BRCA1-BRCT-STOP-rev

<400>  35
caccatccta atgatggtgg tgatgatgac tagtttaggg gatctggggt atcaggta         58


<210>  36
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT-fwd

<400>  36
actttaagaa ggagatatac atatggtgaa ctcctctgct                             40


<210>  37
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT-rev

<400>  37
caccatccta atgatggtgg tgatgatgac tagttggggc cacaacttca aca              53


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E6-fwd

<400>  38
actttaagaa ggagatatac atatggc                                           27


<210>  39
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P19-fwd

<400>  39
actttaagaa ggagatatac atatgctg                                          28


<210>  40
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coupling cassette-rev

<400>  40
caccatccta atgatggtgg tga                                               23


<210>  41
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NusA-fwd

<400>  41
cgacatatga acaaagaaat tttggctgta gt                                     32


<210>  42
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NusA-rev

<400>  42
atacatatgg ctaccagagc cgctacccgc ttcgtcaccg aaccag                      46


<210>  43
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SUMO-fwd

<400>  43
gaccatatgt cggactcaga agtcaat                                           27


<210>  44
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SUMO-rev

<400>  44
atccatatgg ctaccagagc cgctaccacc accaatctgt tctctgt                     47


<210>  45
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mut-PARP1-BRCT-fwd

<400>  45
gagatataca tatggtgaac tcctctgct                                         29


<210>  46
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis-fwd

<400>  46
cccctctaga aataattttg tttaacttta agaaggagat atacatatg                   49


<210>  47
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis-rev

<400>  47
cctaatgatg gtggtgatga tgactagt                                          28


<210>  48
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Library-seq-fwd

<400>  48
tcgtcggcag cgtcagatgt gtataagaga cagaacttta agaaggagat atacatatg        59


<210>  49
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT-int-rev

<400>  49
gtctcgtggg ctcggagatg tgtataagag acagtgaagg ctcttggtgg ag               52


<210>  50
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PARP1-BRCT-int-fwd

<400>  50
tcgtcggcag cgtcagatgt gtataagaga cagaacaagg atgaagtgaa ggc              53


<210>  51
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Library-seq-rev

<400>  51
gtctcgtggg ctcggagatg tgtataagag acagatgatg gtggtgatga tgacta           56


