                         SEQUENCE LISTING

<110>  Hernndez, Frank J.
 
<120>  AGENTS FOR USE IN THE DETECTION OF NUCLEASE ACTIVITY

<130>  P10975PC00

<150>  EP14382411
<151>  2014-10-21

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide 1


<220>
<221>  variation/unsure/modified_base
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  variation/unsure/modified_base
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (7)..(7)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (7)..(11)
<223>  n is a, c, g, t or u

<220>
<221>  variation/unsure/modified_base
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (9)..(9)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (10)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<400>  1
nnnnttnnnn n                                                            11


<210>  2
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide 2


<220>
<221>  variation/unsure/modified_base
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (3)..(11)
<223>  n is a, c, g, t or u

<220>
<221>  variation/unsure/modified_base
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (5)..(5)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (6)..(6)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (7)..(7)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (8)..(8)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (9)..(9)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (10)..(10)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (12)..(12)
<223>  /note=2'O-methyl guanine

<400>  2
ttnnnnnnnn naa                                                          13


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide 3


<220>
<221>  variation/unsure/modified_base
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, t or u

<220>
<221>  variation/unsure/modified_base
<222>  (4)..(4)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (4)..(13)
<223>  n is a, c, g, t or u

<220>
<221>  variation/unsure/modified_base
<222>  (5)..(5)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (6)..(6)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (7)..(7)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (8)..(8)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation/unsure/modified_base
<222>  (9)..(9)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (10)..(10)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (11)..(11)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation/unsure/modified_base
<222>  (12)..(12)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation/unsure/modified_base
<222>  (16)..(16)
<223>  /note=2'O-methyl guanine

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, t or u

<400>  3
ntannnnnnn nnntan                                                       16


<210>  4
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (7)..(7)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (7)..(11)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<400>  4
nnnnttnnnn n                                                            11


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (7)..(7)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (7)..(16)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (12)..(16)
<223>  /note=2'O-methyl uracile

<400>  5
nnnnttnnnn nnnnnn                                                       16


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (7)..(7)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (7)..(36)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (12)..(36)
<223>  /note=2'O-methyl uracile

<400>  6
nnnnttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnn                                 36


<210>  7
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (7)..(7)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (7)..(61)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (12)..(61)
<223>  /note=2'O-methyl uracile

<400>  7
nnnnttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       60

n                                                                       61


<210>  8
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (4)..(6)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (7)..(7)
<223>  /note=2'O-methyl citosine

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<400>  8
nnnnnnnnnn n                                                            11


<210>  9
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl cytosine

<220>
<221>  misc_feature
<222>  (3)..(14)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (4)..(4)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (5)..(5)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (6)..(7)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (8)..(8)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (9)..(10)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (11)..(11)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (12)..(12)
<223>  /note=2'O-methyl guanine

<220>
<221>  variation
<222>  (13)..(14)
<223>  /note=2'O-methyl adenine

<400>  9
ttnnnnnnnn nnnn                                                         14


<210>  10
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  olignoucleotide


<220>
<221>  variation
<222>  (1)..(1)
<223>  /note=2'O-methyl uracile

<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (2)..(2)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (3)..(3)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (4)..(5)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (6)..(6)
<223>  /note=2'O-methyl uracile

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  2' fluoro

<220>
<221>  variation
<222>  (10)..(10)
<223>  /note=2'O-methyl uracile

<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (11)..(12)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (13)..(13)
<223>  /note=2'O-methyl uracile

<220>
<221>  variation
<222>  (14)..(14)
<223>  /note=2'O-methyl cytosine

<220>
<221>  variation
<222>  (15)..(15)
<223>  /note=2'O-methyl uracile

<400>  10
nnnnnnaaan nnnnn                                                        15


<210>  11
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  misc_feature
<223>  2' fluoro

<400>  11
ucucguacgu uc                                                           12


