                         SEQUENCE LISTING

<110>  Monsanto Technology LLC
       Baum, James A
       Cerruti, Thomas
       Flasinski, Stanislaw
       Fu, Xiaoran
       Howe, Arlene R
       Salvador, Sara
 
<120>  Lepidopteran-Active Cry1Da1 Amino Acid Sequence Variant Proteins

<130>  P34223WO00/0022270.00078

<150>  US 62/064994
<151>  2014-10-16

<150>  US 62/065017
<151>  2014-10-17

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  3498
<212>  DNA
<213>  Bacillus thuringiensis


<220>
<221>  misc_feature
<222>  (1)..(3498)
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1.

<400>  1
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttatgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  2
<211>  1165
<212>  PRT
<213>  Bacillus thuringiensis


<220>
<221>  MISC_FEATURE
<222>  (1)..(1165)
<223>  Amino acid sequence of the protein Cry1Da1.

<400>  2

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  3
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1_3.

<400>  3
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgtagtgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  4
<211>  1165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_3.

<400>  4

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  5
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1_4.

<400>  5
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttatgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatc gtaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  6
<211>  1165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_4.

<400>  6

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Arg Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  7
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1_5.

<400>  7
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tagaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  8
<211>  1165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_5.

<400>  8

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Arg 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  9
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1_6.

<400>  9
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaaaatatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tccaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  10
<211>  1165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_6.

<400>  10

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Lys Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Pro 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  11
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding Cry1Da1_7.

<400>  11
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcagtatatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tccaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta     1860

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg     1920

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga     1980

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca     2040

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa     2100

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat     2280

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga     2340

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga     2400

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt     2460

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc     2520

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta     2580

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa     2640

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa     2700

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt     2760

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct     2820

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat     2880

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta     2940

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa     3000

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac     3060

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa     3120

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt     3180

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat     3240

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa     3300

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat     3360

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat     3420

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta     3480

ctccttatgg aggaatag                                                   3498


<210>  12
<211>  1165
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_7.

<400>  12

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Val Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Pro 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  13
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844.

<400>  13
atggagatca acaaccagaa ccagtgcgtc ccgtacaact gcctgagcaa ccctaaggag       60

atcatcctgg gtgaggaacg cctggagacc ggcaacaccg tagccgacat tagcctgggc      120

ctcatcaact tcctctacag caacttcgtg cccggcggtg gcttcatcgt gggcctcctg      180

gagcttatct ggggcttcat cggcccgtcc cagtgggaca tcttcctcgc ccagatcgag      240

caactgatca gccagcggat cgaggagttc gctaggaacc aggccatctc ccgcctggag      300

ggactctcca acctctacaa ggtgtacgtg cgcgcgttca gcgactggga gaaggacccg      360

accaacccgg ccctccgcga ggaaatgcgt atccagttca acgatatgaa ctcggccctc      420

atcaccgcca tcccgctctt ccgcgtgcag aactacgagg tggccctcct gtccgtgtac      480

gttcaagccg ccaacctcca cctctccatc ctccgcgacg tgagcgtgtt cggcgagcgc      540

tggggctacg acaccgccac catcaacaac cgctactccg acctcacctc cctcatccac      600

gtttacacca accactgcgt ggacacgtac aaccagggcc tccgccgcct ggagggccgc      660

ttcctctccg actggatcgt gtacaaccgc ttccgccgcc agctcaccat ctccgtcctg      720

gacatcgtcg ccttctttcc caactacgac atccgcacct accctatcca gaccgccacc      780

cagctcaccc gcgaggtcta cctcgacctc ccgttcatca acgagaacct cagcccggcc      840

gccagctacc cgaccttctc cgccgctgag tccgccatca ttcgcagccc gcacctcgtg      900

gacttcctca actccttcac catctacacc gactccctcg cccgctacgc ctactggggc      960

ggtcacctcg tgaactcctt ccgcaccggc accactacca acctcatccg cagcccgctc     1020

tacggccgcg agggcaacac cgagcgcccg gtgaccatca ccgccagccc gagcgtgccc     1080

atcttccgca ccctcagcta catcaccggc ctggacaaca gcaaccctgt ggcgggcatc     1140

gagggcgtgg agttccagaa caccatctcc aggagcatct accgcaagag cggccctatc     1200

gacagcttca gcgagctgcc tcctcaggac gccagcgtga gccctgccat cggctacagc     1260

cacaggctgt gccacgccac cttcctggag cgcatcagcg gccctcgcat cgcgggcacc     1320

gtgttctcgt ggacccaccg cagcgcctct cctacgaacg aggtgtctcc tagtcgcatc     1380

acccagatcc cttgggtcaa ggcccacacc ctggctagtg gcgctagtgt catcaagggc     1440

cctggcttca ccggtggtga catcctgacc aggaactcta tgggcgagct gggcactctg     1500

agggtcactt tcactggccg cctgcctcag tcttactaca tccgcttccg ctacgctagt     1560

gtcgctaacc gctctggtac tttccgctac tctcagcctc cgtcttacgg tatctctttc     1620

cctaagacta tggacgctgg tgagcctctg accagtagga gcttcgctca cactactctg     1680

ttcactccta tcactttctc tagggctcag gaggagttcg acctgtacat ccagtctggt     1740

gtgtacatcg acaggatcga gttcatcccc gtgaccgcca cgttcgaggc cgagtacgac     1800

cttgagcgcg cccagaaggc tgtcaatgag ctcttcacgt ccagcaatca gatcggcctg     1860

aagaccgacg tcactgacta ccacatcgac caagtctcca acctcgtgga gtgcctctcc     1920

gatgagttct gcctcgacga gaagaaggag ctgtccgaga aggtgaagca tgccaagcgt     1980

ctcagcgacg agaggaatct cctccaggac cccaatttcc gcggcatcaa caggcagctc     2040

gaccgcggct ggcgcggcag caccgacatc acgatccagg gcggcgacga tgtgttcaag     2100

gagaactacg tgactctcct gggcactttc gacgagtgct accctaccta cttgtaccag     2160

aagatcgatg agtccaagct caaggcttac actcgctacc agctccgcgg ctacatcgaa     2220

gacagccaag acctcgagat ttacctgatc cgctacaacg ccaagcacga gaccgtcaac     2280

gtgcccggta ctggttccct ctggccgctg agcgccccca gcccgatcgg caagtgtgcc     2340

caccacagcc accacttctc cttggacatc gatgtgggct gcaccgacct gaacgaggac     2400

ctcggagtct gggtcatctt caagatcaag acccaggacg gccacgcgcg cctgggcaac     2460

ctggagttcc tcgaggagaa gcccctggtc ggtgaggctc tggccagggt caagagggct     2520

gagaagaagt ggagggacaa gcgcgagaag ctcgagtggg agaccaacat cgtttacaag     2580

gaggccaagg agagcgtcga cgccctgttc gtgaactccc agtacgaccg cctgcaggcc     2640

gacaccaaca tcgccatgat ccacgctgcc gacaagaggg tgcacagcat tcgcgaggcc     2700

tacctgcctg agctgtccgt gatccctggt gtgaacgctg ccatctttga ggagctggag     2760

ggccgcatct ttaccgcatt ctccctgtac gacgcccgca acgtgatcaa gaacggtgac     2820

ttcaacaatg gcctcagctg ctggaacgtc aagggccacg tggacgtcga ggaacagaac     2880

aaccaccgct ccgtcctggt cgtcccagag tgggaggctg aggtctccca agaggtccgc     2940

gtctgcccag gccgcggcta cattctcagg gtcaccgctt acaaggaggg ctacggtgag     3000

ggctgtgtga ccatccacga gatcgagaac aacaccgacg agcttaagtt ctccaactgc     3060

gtggaggagg aggtgtaccc aaacaacacc gttacttgca acgactacac cgccacccag     3120

gaggagtacg agggcaccta cacttccagg aacaggggct acgatggtgc ctacgagagc     3180

aacagcagcg ttcctgctga ctacgcttcc gcctacgagg agaaggccta cacggatggc     3240

cgcagggaca acccttgcga gagcaaccgc ggctacggcg actacactcc cctgcccgcc     3300

ggctacgtta ccaaggagct ggagtacttc ccggagactg acaaggtgtg gatcgagatc     3360

ggcgagaccg agggcacctt catcgtggac agcgtggagc tgctcctgat ggaggagtag     3420


<210>  14
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the chimeric protein TIC844.

<400>  14

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  15
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_2.

<400>  15
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatt cacatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggttaagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  16
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_2.

<400>  16

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser His Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Leu 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  17
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_4.

<400>  17
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgtagtgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  18
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_4.

<400>  18

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  19
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_5.

<400>  19
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaaaatatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tccaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  20
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_5.

<400>  20

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Lys Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Pro 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  21
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_6.

<400>  21
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttatgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatc gtaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  22
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_6.

<400>  22

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Arg Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  23
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_7.

<400>  23
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tagaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  24
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_7.

<400>  24

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Arg 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  25
<211>  3420
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence used for expression in a bacterial cell 
       encoding TIC844_8.

<400>  25
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcagtatatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttctgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tccaacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat     1800

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta     1860

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct     1920

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga     1980

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta     2040

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa     2100

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa     2160

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa     2220

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat     2280

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc     2340

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac     2400

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat     2460

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg     2520

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa     2580

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg     2640

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct     2700

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa     2760

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat     2820

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac     2880

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt     2940

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa     3000

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt     3060

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa     3120

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc     3180

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga     3240

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct     3300

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc     3360

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag     3420


<210>  26
<211>  1139
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_8.

<400>  26

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Val Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Pro 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  27
<211>  3498
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1.

<400>  27
atggagatca acaaccagaa ccagtgcgtc ccgtacaact gcctgagcaa ccctaaggag       60

atcatcctgg gtgaggaacg cctggagacc ggcaacaccg tagccgacat tagcctgggc      120

ctcatcaact tcctctacag caacttcgtg cccggcggtg gcttcatcgt gggcctcctg      180

gagcttatct ggggcttcat cggcccgtcc cagtgggaca tcttcctcgc ccagatcgag      240

caactgatca gccagcggat cgaggagttc gctaggaacc aggccatctc ccgcctggag      300

ggactctcca acctctacaa ggtgtacgtg cgcgcgttca gcgactggga gaaggacccg      360

accaacccgg ccctccgcga ggaaatgcgt atccagttca acgatatgaa ctcggccctc      420

atcaccgcca tcccgctctt ccgcgtgcag aactacgagg tggccctcct gtccgtgtac      480

gttcaagccg ccaacctcca cctctccatc ctccgcgacg tgagcgtgtt cggcgagcgc      540

tggggctacg acaccgccac catcaacaac cgctactccg acctcacctc cctcatccac      600

gtttacacca accactgcgt ggacacgtac aaccagggcc tccgccgcct ggagggccgc      660

ttcctctccg actggatcgt gtacaaccgc ttccgccgcc agctcaccat ctccgtcctg      720

gacatcgtcg ccttctttcc caactacgac atccgcacct accctatcca gaccgccacc      780

cagctcaccc gcgaggtcta cctcgacctc ccgttcatca acgagaacct cagcccggcc      840

gccagctacc cgaccttctc cgccgctgag tccgccatca ttcgcagccc gcacctcgtg      900

gacttcctca actccttcac catctacacc gactccctcg cccgctacgc ctactggggc      960

ggtcacctcg tgaactcctt ccgcaccggc accactacca acctcatccg cagcccgctc     1020

tacggccgcg agggcaacac cgagcgcccg gtgaccatca ccgccagccc gagcgtgccc     1080

atcttccgca ccctcagcta catcaccggc ctggacaaca gcaaccctgt ggcgggcatc     1140

gagggcgtgg agttccagaa caccatctcc aggagcatct accgcaagag cggccctatc     1200

gacagcttca gcgagctgcc tcctcaggac gccagcgtga gccctgccat cggctacagc     1260

cacaggctgt gccacgccac cttcctggag cgcatcagcg gccctcgcat cgcgggcacc     1320

gtgttctcgt ggacccaccg cagcgcctct cctacgaacg aggtgtctcc tagtcgcatc     1380

acccagatcc cttgggtcaa ggcccacacc ctggctagtg gcgctagtgt catcaagggc     1440

cctggcttca ccggtggtga catcctgacc aggaactcta tgggcgagct gggcactctg     1500

agggtcactt tcactggccg cctgcctcag tcttactaca tccgcttccg ctacgctagt     1560

gtcgctaacc gctctggtac tttccgctac tctcagcctc cgtcttacgg tatctctttc     1620

cctaagacta tggacgctgg tgagcctctg accagtagga gcttcgctca cactactctg     1680

ttcactccta tcactttctc tagggctcag gaggagttcg acctgtacat ccagtctggt     1740

gtgtacatcg acaggatcga gttcatcccc gtgaccgcca cgttcgaggc cgagtacgac     1800

cttgagcgcg cccagaaggt ggtgaacgcc ctcttcacta gcactaacca gctaggcctg     1860

aagactgacg tgaccgacta ccacatcgac caagtgagca acctagtggc ctgcctctcc     1920

gacgagttct gcctcgacga gaagcgcgag ctgtccgaga aggtgaagca cgccaagcgc     1980

ctctccgacg agcgcaacct gctccaggac cccaacttca ggggcatcaa caggcagccc     2040

gaccgcggct ggcgcggctc caccgacatc accatccagg gcggtgacga cgtattcaag     2100

gagaactacg ttaccctccc cggcaccttc gacgagtgtt accccaccta cctctaccag     2160

aagatcgacg agtccaagct gaaggcctac acccgctacc agctccgcgg ctacatcgag     2220

gactcccagg acctggaaat ctacctcatc cgctacaacg ccaagcacga gatcgtgaac     2280

gtgcctggca ccggcagcct ctggcctctc agcgtggaga accagatcgg cccttgcggc     2340

gagcctaacc gctgcgcccc tcacctcgag tggaaccctg acctccactg ctcgtgcagg     2400

gacggcgaga agtgcgccca ccatagccac cacttctctc tggacatcga cgtgggctgc     2460

accgacctga acgaggacct gggcgtgtgg gttatcttca agatcaagac ccaggacggt     2520

cacgccaggc tgggtaacct ggagttcctt gaggaaaagc ctctgctggg tgaggccctg     2580

gccagggtca agagggctga gaagaaatgg agggataaga gggagaccct gcagctggag     2640

accactatcg tctacaagga ggctaaggag tctgtcgatg ctctgttcgt caactctcag     2700

tacgatagac tgcaagctga taccaacatc gctatgatcc acgctgcgga taagcgggtc     2760

caccggatcc gggaggctta ccttccggag ctttctgtca tcccgggtgt caacgctgcg     2820

atcttcgagg aacttgagga acggatcttc actgcgttta gtctttacga tgcgcggaac     2880

atcatcaaga acggggactt caacaatggt ctgctgtgct ggaacgtcaa gggtcatgtc     2940

gaggtcgagg aacaaaacaa tcatcgtagt gtccttgtca ttcctgagtg ggaggcggag     3000

gtctctcaag aggtccgtgt ttgcccgggg cgtgggtaca ttcttcgtgt tactgcgtac     3060

aaggaggggt acggggaggg gtgcgttact attcatgaga ttgagaacaa tactgatgag     3120

cttaagttca acaattgtgt tgaggaggag gtttacccga acaatactgt tacgtgcatc     3180

aactacacgg caacgcaaga ggaatacgag gggacgtaca cctcgcgtaa tagagggtat     3240

gatgaggcgt acggaaacaa cccgtcggtt ccagcagatt atgcctcggt ttatgaggag     3300

aagtcgtaca cggatagacg acgcgagaat ccatgtgagt caaatcgagg atacggagat     3360

tacacaccat taccagcagg atacgttaca aaggagttgg aatacttccc ggaaacagat     3420

aaagtttgga ttgaaatcgg agaaacagaa ggaacattca tcgtcgactc agtagaattg     3480

ttgttgatgg aagaatga                                                   3498


<210>  28
<211>  1165
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of Cry1Da1 encoded by a synthetic DNA 
       sequence.

<400>  28

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 
    770                 775                 780                 


Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg 
785                 790                 795                 800 


Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 
                805                 810                 815     


Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 
            820                 825                 830         


Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 
        835                 840                 845             


Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 
    850                 855                 860                 


Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu 
865                 870                 875                 880 


Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 
                885                 890                 895     


Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 
            900                 905                 910         


Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 
        915                 920                 925             


Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 
    930                 935                 940                 


Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 
945                 950                 955                 960 


Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 
                965                 970                 975     


Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 
            980                 985                 990         


Val Ile Pro Glu Trp Glu Ala Glu  Val Ser Gln Glu Val  Arg Val Cys 
        995                 1000                 1005             


Pro Gly  Arg Gly Tyr Ile Leu  Arg Val Thr Ala Tyr  Lys Glu Gly 
    1010                 1015                 1020             


Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu Ile Glu  Asn Asn Thr 
    1025                 1030                 1035             


Asp Glu  Leu Lys Phe Asn Asn  Cys Val Glu Glu Glu  Val Tyr Pro 
    1040                 1045                 1050             


Asn Asn  Thr Val Thr Cys Ile  Asn Tyr Thr Ala Thr  Gln Glu Glu 
    1055                 1060                 1065             


Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg Gly Tyr  Asp Glu Ala 
    1070                 1075                 1080             


Tyr Gly  Asn Asn Pro Ser Val  Pro Ala Asp Tyr Ala  Ser Val Tyr 
    1085                 1090                 1095             


Glu Glu  Lys Ser Tyr Thr Asp  Arg Arg Arg Glu Asn  Pro Cys Glu 
    1100                 1105                 1110             


Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro Leu Pro  Ala Gly Tyr 
    1115                 1120                 1125             


Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu Thr Asp  Lys Val Trp 
    1130                 1135                 1140             


Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe Ile Val  Asp Ser Val 
    1145                 1150                 1155             


Glu Leu  Leu Leu Met Glu Glu  
    1160                 1165 


<210>  29
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1 with an additional Alanine residue inserted at position 
       2.

<400>  29
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcta cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctacatcacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  30
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of Cry1Da1 encoded by a synthetic DNA 
       sequence wherein an additional Alanine residue has been inserted 
       at position 2.

<400>  30

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Ile Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  31
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1_3 with an additional Alanine residue inserted at position
       2.

<400>  31
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcag cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctacatcacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  32
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_3 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  32

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Ile Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  33
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1_4 with an additional Alanine residue inserted at position
       2.

<400>  33
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcta cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctacatcacc ggcctggaca acaggaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  34
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_4 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  34

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Ile Thr Gly Leu Asp Asn Arg Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  35
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1_5 with an additional Alanine residue inserted at position
       2.

<400>  35
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcag cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctaccgcacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  36
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_5 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  36

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Arg Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  37
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1_6 with an additional Alanine residue inserted at position
       2.

<400>  37
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccaagt acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcag cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctaccccacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  38
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_6 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  38

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Lys Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Pro Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  39
<211>  3501
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       Cry1Da1_7 with an additional Alanine residue inserted at position
       2.

<400>  39
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccgtct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcag cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctaccccacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggtggtgaac gccctcttca ctagcactaa ccagctaggc     1860

ctgaagactg acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc     1920

tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag     1980

cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag     2040

cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc     2100

aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac ctacctctac     2160

cagaagatcg acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc     2220

gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg     2280

aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc     2340

ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc     2400

agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat cgacgtgggc     2460

tgcaccgacc tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac     2520

ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc     2580

ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg     2640

gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct     2700

cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc ggataagcgg     2760

gtccaccgga tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct     2820

gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg     2880

aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat     2940

gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg     3000

gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg tgttactgcg     3060

tacaaggagg ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat     3120

gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc     3180

atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg     3240

tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag     3300

gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg aggatacgga     3360

gattacacac cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca     3420

gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa     3480

ttgttgttga tggaagaatg a                                               3501


<210>  40
<211>  1166
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the engineered insecticidal protein 
       Cry1Da1_7 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  40

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Val Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Pro Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 
        595                 600                 605             


Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn 
    770                 775                 780                 


Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys 
785                 790                 795                 800 


Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp 
                805                 810                 815     


Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 
            820                 825                 830         


Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 
        835                 840                 845             


Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 
    850                 855                 860                 


Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu 
865                 870                 875                 880 


Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 
                885                 890                 895     


Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 
            900                 905                 910         


Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 
        915                 920                 925             


Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 
    930                 935                 940                 


Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg 
945                 950                 955                 960 


Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 
                965                 970                 975     


Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 
            980                 985                 990         


Leu Val Ile Pro Glu Trp Glu Ala  Glu Val Ser Gln Glu  Val Arg Val 
        995                 1000                 1005             


Cys Pro  Gly Arg Gly Tyr Ile  Leu Arg Val Thr Ala  Tyr Lys Glu 
    1010                 1015                 1020             


Gly Tyr  Gly Glu Gly Cys Val  Thr Ile His Glu Ile  Glu Asn Asn 
    1025                 1030                 1035             


Thr Asp  Glu Leu Lys Phe Asn  Asn Cys Val Glu Glu  Glu Val Tyr 
    1040                 1045                 1050             


Pro Asn  Asn Thr Val Thr Cys  Ile Asn Tyr Thr Ala  Thr Gln Glu 
    1055                 1060                 1065             


Glu Tyr  Glu Gly Thr Tyr Thr  Ser Arg Asn Arg Gly  Tyr Asp Glu 
    1070                 1075                 1080             


Ala Tyr  Gly Asn Asn Pro Ser  Val Pro Ala Asp Tyr  Ala Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Lys Ser Tyr Thr  Asp Arg Arg Arg Glu  Asn Pro Cys 
    1100                 1105                 1110             


Glu Ser  Asn Arg Gly Tyr Gly  Asp Tyr Thr Pro Leu  Pro Ala Gly 
    1115                 1120                 1125             


Tyr Val  Thr Lys Glu Leu Glu  Tyr Phe Pro Glu Thr  Asp Lys Val 
    1130                 1135                 1140             


Trp Ile  Glu Ile Gly Glu Thr  Glu Gly Thr Phe Ile  Val Asp Ser 
    1145                 1150                 1155             


Val Glu  Leu Leu Leu Met Glu  Glu 
    1160                 1165     


<210>  41
<211>  3423
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       TIC844 with an additional Alanine residue inserted at position 2.

<400>  41
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcta cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctacatcacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggctgtcaat gagctcttca cgtccagcaa tcagatcggc     1860

ctgaagaccg acgtcactga ctaccacatc gaccaagtct ccaacctcgt ggagtgcctc     1920

tccgatgagt tctgcctcga cgagaagaag gagctgtccg agaaggtgaa gcatgccaag     1980

cgtctcagcg acgagaggaa tctcctccag gaccccaatt tccgcggcat caacaggcag     2040

ctcgaccgcg gctggcgcgg cagcaccgac atcacgatcc agggcggcga cgatgtgttc     2100

aaggagaact acgtgactct cctgggcact ttcgacgagt gctaccctac ctacttgtac     2160

cagaagatcg atgagtccaa gctcaaggct tacactcgct accagctccg cggctacatc     2220

gaagacagcc aagacctcga gatttacctg atccgctaca acgccaagca cgagaccgtc     2280

aacgtgcccg gtactggttc cctctggccg ctgagcgccc ccagcccgat cggcaagtgt     2340

gcccaccaca gccaccactt ctccttggac atcgatgtgg gctgcaccga cctgaacgag     2400

gacctcggag tctgggtcat cttcaagatc aagacccagg acggccacgc gcgcctgggc     2460

aacctggagt tcctcgagga gaagcccctg gtcggtgagg ctctggccag ggtcaagagg     2520

gctgagaaga agtggaggga caagcgcgag aagctcgagt gggagaccaa catcgtttac     2580

aaggaggcca aggagagcgt cgacgccctg ttcgtgaact cccagtacga ccgcctgcag     2640

gccgacacca acatcgccat gatccacgct gccgacaaga gggtgcacag cattcgcgag     2700

gcctacctgc ctgagctgtc cgtgatccct ggtgtgaacg ctgccatctt tgaggagctg     2760

gagggccgca tctttaccgc attctccctg tacgacgccc gcaacgtgat caagaacggt     2820

gacttcaaca atggcctcag ctgctggaac gtcaagggcc acgtggacgt cgaggaacag     2880

aacaaccacc gctccgtcct ggtcgtccca gagtgggagg ctgaggtctc ccaagaggtc     2940

cgcgtctgcc caggccgcgg ctacattctc agggtcaccg cttacaagga gggctacggt     3000

gagggctgtg tgaccatcca cgagatcgag aacaacaccg acgagcttaa gttctccaac     3060

tgcgtggagg aggaggtgta cccaaacaac accgttactt gcaacgacta caccgccacc     3120

caggaggagt acgagggcac ctacacttcc aggaacaggg gctacgatgg tgcctacgag     3180

agcaacagca gcgttcctgc tgactacgct tccgcctacg aggagaaggc ctacacggat     3240

ggccgcaggg acaacccttg cgagagcaac cgcggctacg gcgactacac tcccctgccc     3300

gccggctacg ttaccaagga gctggagtac ttcccggaga ctgacaaggt gtggatcgag     3360

atcggcgaga ccgagggcac cttcatcgtg gacagcgtgg agctgctcct gatggaggag     3420

tag                                                                   3423


<210>  42
<211>  1140
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the chimeric protein TIC844 encoded by a 
       synthetic DNA sequence wherein an additional Alanine residue has 
       been inserted at position 2.

<400>  42

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Ile Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala 
        595                 600                 605             


Val Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser 
    770                 775                 780                 


His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu 
785                 790                 795                 800 


Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His 
                805                 810                 815     


Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly 
            820                 825                 830         


Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys 
        835                 840                 845             


Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys 
    850                 855                 860                 


Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln 
865                 870                 875                 880 


Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His 
                885                 890                 895     


Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val 
            900                 905                 910         


Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe 
        915                 920                 925             


Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn 
    930                 935                 940                 


Gly Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln 
945                 950                 955                 960 


Asn Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val 
                965                 970                 975     


Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val 
            980                 985                 990         


Thr Ala Tyr Lys Glu Gly Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu 
        995                 1000                 1005             


Ile Glu  Asn Asn Thr Asp Glu  Leu Lys Phe Ser Asn  Cys Val Glu 
    1010                 1015                 1020             


Glu Glu  Val Tyr Pro Asn Asn  Thr Val Thr Cys Asn  Asp Tyr Thr 
    1025                 1030                 1035             


Ala Thr  Gln Glu Glu Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg 
    1040                 1045                 1050             


Gly Tyr  Asp Gly Ala Tyr Glu  Ser Asn Ser Ser Val  Pro Ala Asp 
    1055                 1060                 1065             


Tyr Ala  Ser Ala Tyr Glu Glu  Lys Ala Tyr Thr Asp  Gly Arg Arg 
    1070                 1075                 1080             


Asp Asn  Pro Cys Glu Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro 
    1085                 1090                 1095             


Leu Pro  Ala Gly Tyr Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu 
    1100                 1105                 1110             


Thr Asp  Lys Val Trp Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe 
    1115                 1120                 1125             


Ile Val  Asp Ser Val Glu Leu  Leu Leu Met Glu Glu  
    1130                 1135                 1140 


<210>  43
<211>  3423
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic DNA sequence designed for plant expression encoding 
       TIC844_8 with an additional Alanine residue inserted at position 
       2.

<400>  43
atggctgaga tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag       60

gagatcatcc tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg      120

ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc      180

ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc      240

gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat ctcccgcctg      300

gagggactct ccaacctcta caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac      360

ccgaccaacc cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc      420

ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg      480

tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag      540

cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac ctccctcatc      600

cacgtttaca ccaaccactg cgtggacacg tacaaccagg gcctccgccg cctggagggc      660

cgcttcctct ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc      720

ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc      780

acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg      840

gccgccgtct acccgacctt ctccgccgct gagtccgcca tcattcgcag cccgcacctc      900

gtggacttcc tcaactcctt caccatctac accgactccc tcgcccgcag cgcctactgg      960

ggcggtcacc tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg     1020

ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg     1080

cccatcttcc gcaccctcag ctaccccacc ggcctggaca acagcaaccc tgtggcgggc     1140

atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa gagcggccct     1200

atcgacagct tcagcgagct gcctcctcag gacgccagcg tgagccctgc catcggctac     1260

agccacaggc tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc     1320

accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc     1380

atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag     1440

ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga gctgggcact     1500

ctgagggtca ctttcactgg ccgcctgcct cagtcttact acatccgctt ccgctacgct     1560

agtgtcgcta accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct     1620

ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact     1680

ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct     1740

ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga ggccgagtac     1800

gaccttgagc gcgcccagaa ggctgtcaat gagctcttca cgtccagcaa tcagatcggc     1860

ctgaagaccg acgtcactga ctaccacatc gaccaagtct ccaacctcgt ggagtgcctc     1920

tccgatgagt tctgcctcga cgagaagaag gagctgtccg agaaggtgaa gcatgccaag     1980

cgtctcagcg acgagaggaa tctcctccag gaccccaatt tccgcggcat caacaggcag     2040

ctcgaccgcg gctggcgcgg cagcaccgac atcacgatcc agggcggcga cgatgtgttc     2100

aaggagaact acgtgactct cctgggcact ttcgacgagt gctaccctac ctacttgtac     2160

cagaagatcg atgagtccaa gctcaaggct tacactcgct accagctccg cggctacatc     2220

gaagacagcc aagacctcga gatttacctg atccgctaca acgccaagca cgagaccgtc     2280

aacgtgcccg gtactggttc cctctggccg ctgagcgccc ccagcccgat cggcaagtgt     2340

gcccaccaca gccaccactt ctccttggac atcgatgtgg gctgcaccga cctgaacgag     2400

gacctcggag tctgggtcat cttcaagatc aagacccagg acggccacgc gcgcctgggc     2460

aacctggagt tcctcgagga gaagcccctg gtcggtgagg ctctggccag ggtcaagagg     2520

gctgagaaga agtggaggga caagcgcgag aagctcgagt gggagaccaa catcgtttac     2580

aaggaggcca aggagagcgt cgacgccctg ttcgtgaact cccagtacga ccgcctgcag     2640

gccgacacca acatcgccat gatccacgct gccgacaaga gggtgcacag cattcgcgag     2700

gcctacctgc ctgagctgtc cgtgatccct ggtgtgaacg ctgccatctt tgaggagctg     2760

gagggccgca tctttaccgc attctccctg tacgacgccc gcaacgtgat caagaacggt     2820

gacttcaaca atggcctcag ctgctggaac gtcaagggcc acgtggacgt cgaggaacag     2880

aacaaccacc gctccgtcct ggtcgtccca gagtgggagg ctgaggtctc ccaagaggtc     2940

cgcgtctgcc caggccgcgg ctacattctc agggtcaccg cttacaagga gggctacggt     3000

gagggctgtg tgaccatcca cgagatcgag aacaacaccg acgagcttaa gttctccaac     3060

tgcgtggagg aggaggtgta cccaaacaac accgttactt gcaacgacta caccgccacc     3120

caggaggagt acgagggcac ctacacttcc aggaacaggg gctacgatgg tgcctacgag     3180

agcaacagca gcgttcctgc tgactacgct tccgcctacg aggagaaggc ctacacggat     3240

ggccgcaggg acaacccttg cgagagcaac cgcggctacg gcgactacac tcccctgccc     3300

gccggctacg ttaccaagga gctggagtac ttcccggaga ctgacaaggt gtggatcgag     3360

atcggcgaga ccgagggcac cttcatcgtg gacagcgtgg agctgctcct gatggaggag     3420

tag                                                                   3423


<210>  44
<211>  1140
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of the engineered insecticidal chimeric 
       protein TIC844_8 encoded by a synthetic DNA sequence wherein an 
       additional Alanine residue has been inserted at position 2.

<400>  44

Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 
            20                  25                  30          


Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 
        35                  40                  45              


Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 
    50                  55                  60                  


Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 
            100                 105                 110         


Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 
            180                 185                 190         


Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 
        195                 200                 205             


Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 
    210                 215                 220                 


Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val 
225                 230                 235                 240 


Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 
                245                 250                 255     


Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 
            260                 265                 270         


Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Val Tyr Pro Thr Phe Ser 
        275                 280                 285             


Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 
    290                 295                 300                 


Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp 
305                 310                 315                 320 


Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 
                325                 330                 335     


Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 
            340                 345                 350         


Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 
        355                 360                 365             


Pro Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 
    370                 375                 380                 


Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro 
385                 390                 395                 400 


Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 
                405                 410                 415     


Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg 
            420                 425                 430         


Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 
        435                 440                 445             


Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 
    450                 455                 460                 


Pro Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys 
465                 470                 475                 480 


Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 
                485                 490                 495     


Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 
            500                 505                 510         


Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 
        515                 520                 525             


Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 
    530                 535                 540                 


Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr 
545                 550                 555                 560 


Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 
                565                 570                 575     


Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 
            580                 585                 590         


Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala 
        595                 600                 605             


Val Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp 
    610                 615                 620                 


Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu 
625                 630                 635                 640 


Ser Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val 
                645                 650                 655     


Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro 
            660                 665                 670         


Asn Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser 
        675                 680                 685             


Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 
    690                 695                 700                 


Val Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr 
705                 710                 715                 720 


Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 
                725                 730                 735     


Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 
            740                 745                 750         


Tyr Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu 
        755                 760                 765             


Trp Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser 
    770                 775                 780                 


His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu 
785                 790                 795                 800 


Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His 
                805                 810                 815     


Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly 
            820                 825                 830         


Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys 
        835                 840                 845             


Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys 
    850                 855                 860                 


Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln 
865                 870                 875                 880 


Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His 
                885                 890                 895     


Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val 
            900                 905                 910         


Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe 
        915                 920                 925             


Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn 
    930                 935                 940                 


Gly Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln 
945                 950                 955                 960 


Asn Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val 
                965                 970                 975     


Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val 
            980                 985                 990         


Thr Ala Tyr Lys Glu Gly Tyr Gly  Glu Gly Cys Val Thr  Ile His Glu 
        995                 1000                 1005             


Ile Glu  Asn Asn Thr Asp Glu  Leu Lys Phe Ser Asn  Cys Val Glu 
    1010                 1015                 1020             


Glu Glu  Val Tyr Pro Asn Asn  Thr Val Thr Cys Asn  Asp Tyr Thr 
    1025                 1030                 1035             


Ala Thr  Gln Glu Glu Tyr Glu  Gly Thr Tyr Thr Ser  Arg Asn Arg 
    1040                 1045                 1050             


Gly Tyr  Asp Gly Ala Tyr Glu  Ser Asn Ser Ser Val  Pro Ala Asp 
    1055                 1060                 1065             


Tyr Ala  Ser Ala Tyr Glu Glu  Lys Ala Tyr Thr Asp  Gly Arg Arg 
    1070                 1075                 1080             


Asp Asn  Pro Cys Glu Ser Asn  Arg Gly Tyr Gly Asp  Tyr Thr Pro 
    1085                 1090                 1095             


Leu Pro  Ala Gly Tyr Val Thr  Lys Glu Leu Glu Tyr  Phe Pro Glu 
    1100                 1105                 1110             


Thr Asp  Lys Val Trp Ile Glu  Ile Gly Glu Thr Glu  Gly Thr Phe 
    1115                 1120                 1125             


Ile Val  Asp Ser Val Glu Leu  Leu Leu Met Glu Glu  
    1130                 1135                 1140 


