                         SEQUENCE LISTING

<110>  ABAD, ANDRE R.
       CROW, ANDREW CARL
       POLAND, BRAD
       SHI, XIAOMEI
       WOLFE, THOMAS CHAD

 
<120>  INSECTICIDAL POLYPEPTIDES HAVING BROAD SPECTRUM ACTIVITY AND USES THEREOF


<130>  6059-WO-PCT

<150>  US 62/064848
<151>  2014-10-16

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  2256
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  1
atggagggaa ataatctaaa tcaatgcata ccttacaatt gtttaagtaa tcctaaggac       60

ataatattag gtgatgaaag gctagaaact ggtaatactg tagcagacat taccttaggg      120

attgtcaatc tattgttttc tgagtttgtt cctggtggag gctttatact aggattactg      180

gatttaatat gggggtctat aggtcgttcc caatgggatc tatttctgga acagattgaa      240

caattgatta agcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagcg atctttataa gacctatgct agagcgttta gcgattggga ggcagatccg      360

actaatccag cattaaggga agaaatgcgt atacaattta atgacatgaa tagtgctatc      420

ataacggctc tcccactttt tagagttcaa aattatgaag ttgctctttt atctgtttat      480

gttcaagctg caaacttaca tttatctatt ttaagagatg tttcagtctt tggagaaaga      540

tggggatatg atacagcaac tatcaataat cgctatagtg acttaactag ccttattcat      600

gtttatacta atcattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttaccg attggattgt atataatcgt ttccgaagac aattaacaat ttcagtatta      720

gatattgttg cattttttcc aaattatgat attcgaacat atccaattca aacagctact      780

cagctaacga gggaaatcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc cctcattctc agatgctgaa agtgctataa tcaggagtcc tcatttagtg      900

gactttttaa atagcttcac tatttataca gatagtcttg ctcgatattt atattgggga      960

gggcatcggg tgaattttac ccgttcagga gttactactt ttatacaatc accactatat     1020

ggaagggaag gaaatgcaga gcgttctgta attatttcgg catcatctag cgtaccaata     1080

tttagaacac tttcatatgt tactggcctt gacaatgcaa atcctgtagc tggaattgaa     1140

ggagtggaat tccaaaatac tataagtaga agtatctatc gtaaaagtgg tccaattgat     1200

tcttttaatg aattaccacc tcaagatgcc agtgtatctc cttcaattgg gtatagtcac     1260

cgtttatgtc atgccacatt tttagaacgg attagtggac caagaattgc aggtgtcgtt     1320

ttttcctgga cacatcgtag tgctagccct actaatgaag taagttcatc tagaattaca     1380

caaattccat gggtaaaggc gcatactctt gcgtctggtg cctccgttat taaagggcct     1440

ggatttacag gtggagatat actaactagg aataccttag gcgaactggg gactttaaga     1500

gtaacttttg caggaagatt atcacaaagt tattatatac gtttccgtta tgcttccgta     1560

gctaatagga gtggtatatt tagctattca cagccaactt catatggaat ttcctttcca     1620

aaaactatgg atgcagatga atcattaaca tctcgttcat ttgcacttgc tacacttgct     1680

acaccgctaa cctttagaag gcaagaagaa ttaaatctac aaataccatc aggtacttat     1740

atagatcgaa ttgagtttgt tccagtcgat gaaaccttta caacagaatc tgatctggat     1800

agagcacaac aggcggtgaa tgcgctgttt acttcttcca atcaaatcgg cttaaaaaca     1860

gatgtgacgg attatcatat tgatcaagta tccaatttag tggattgttt atcggatgaa     1920

ttttgtctgg atgaaaagcg agaattgtcc gagaaagtca aacatgcgaa gcgactcagt     1980

gatgagcgga atttacttca agatccaaac tttagaggaa tcaatagaca accagaccgt     2040

ggttggagag gaagtacaga tattaccatc caaggaggag atgacgtatt caaagagaat     2100

tacgttacac taccaggtac ctttgatgag tgctatccaa cgtatttata tcaaaaaata     2160

gatgagtcga aattaaaagc ctatacccgt taccaattaa gagggtatat cgaagatagt     2220

caagacttag aaatctattt aattcgctac aattga                               2256


<210>  2
<211>  751
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL aa

<400>  2

Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Ser Glu 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp 
    50                  55                  60                  


Gly Ser Ile Gly Arg Ser Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly 
305                 310                 315                 320 


Gly His Arg Val Asn Phe Thr Arg Ser Gly Val Thr Thr Phe Ile Gln 
                325                 330                 335     


Ser Pro Leu Tyr Gly Arg Glu Gly Asn Ala Glu Arg Ser Val Ile Ile 
            340                 345                 350         


Ser Ala Ser Ser Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr 
        355                 360                 365             


Gly Leu Asp Asn Ala Asn Pro Val Ala Gly Ile Glu Gly Val Glu Phe 
    370                 375                 380                 


Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp 
385                 390                 395                 400 


Ser Phe Asn Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ser Ile 
                405                 410                 415     


Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile Ser 
            420                 425                 430         


Gly Pro Arg Ile Ala Gly Val Val Phe Ser Trp Thr His Arg Ser Ala 
        435                 440                 445             


Ser Pro Thr Asn Glu Val Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp 
    450                 455                 460                 


Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly Pro 
465                 470                 475                 480 


Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu 
                485                 490                 495     


Gly Thr Leu Arg Val Thr Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr 
            500                 505                 510         


Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Ile Phe Ser 
        515                 520                 525             


Tyr Ser Gln Pro Thr Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp 
    530                 535                 540                 


Ala Asp Glu Ser Leu Thr Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala 
545                 550                 555                 560 


Thr Pro Leu Thr Phe Arg Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro 
                565                 570                 575     


Ser Gly Thr Tyr Ile Asp Arg Ile Glu Phe Val Pro Val Asp Glu Thr 
            580                 585                 590         


Phe Thr Thr Glu Ser Asp Leu Asp Arg Ala Gln Gln Ala Val Asn Ala 
        595                 600                 605             


Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp 
    610                 615                 620                 


Tyr His Ile Asp Gln Val Ser Asn Leu Val Asp Cys Leu Ser Asp Glu 
625                 630                 635                 640 


Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala 
                645                 650                 655     


Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg 
            660                 665                 670         


Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile 
        675                 680                 685             


Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu 
    690                 695                 700                 


Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile 
705                 710                 715                 720 


Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr 
                725                 730                 735     


Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn 
            740                 745                 750     


<210>  3
<211>  3492
<212>  DNA
<213>  Bacillus thuringiensis

<400>  3
atggagggaa ataatctaaa tcaatgcata ccttacaatt gtttaagtaa tcctaaggac       60

ataatattag gtgatgaaag gctagaaact ggtaatactg tagcagacat taccttaggg      120

attgtcaatc tattgttttc tgagtttgtt cctggtggag gctttatact aggattactg      180

gatttaatat gggggtctat aggtcgttcc caatgggatc tatttctgga acagattgaa      240

caattgatta agcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagcg atctttataa gacctatgct agagcgttta gcgattggga ggcagatccg      360

actaatccag cattaaggga agaaatgcgt atacaattta atgacatgaa tagtgctatc      420

ataacggctc tcccactttt tagagttcaa aattatgaag ttgctctttt atctgtttat      480

gttcaagctg caaacttaca tttatctatt ttaagagatg tttcagtctt tggagaaaga      540

tggggatatg atacagcaac tatcaataat cgctatagtg acttaactag ccttattcat      600

gtttatacta atcattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttaccg attggattgt atataatcgt ttccgaagac aattaacaat ttcagtatta      720

gatattgttg cattttttcc aaattatgat attcgaacat atccaattca aacagctact      780

cagctaacga gggaaatcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc cctcattctc agatgctgaa agtgctataa tcaggagtcc tcatttagtg      900

gactttttaa atagcttcac tatttataca gatagtcttg ctcgatattt atattgggga      960

gggcatcggg tgaattttac ccgttcagga gttactactt ttatacaatc accactatat     1020

ggaagggaag gaaatgcaga gcgttctgta attatttcgg catcatctag cgtaccaata     1080

tttagaacac tttcatatgt tactggcctt gacaatgcaa atcctgtagc tggaattgaa     1140

ggagtggaat tccaaaatac tataagtaga agtatctatc gtaaaagtgg tccaattgat     1200

tcttttaatg aattaccacc tcaagatgcc agtgtatctc cttcaattgg gtatagtcac     1260

cgtttatgtc atgccacatt tttagaacgg attagtggac caagaattgc aggtgtcgtt     1320

ttttcctgga cacatcgtag tgctagccct actaatgaag taagttcatc tagaattaca     1380

caaattccat gggtaaaggc gcatactctt gcgtctggtg cctccgttat taaagggcct     1440

ggatttacag gtggagatat actaactagg aataccttag gcgaactggg gactttaaga     1500

gtaacttttg caggaagatt atcacaaagt tattatatac gtttccgtta tgcttccgta     1560

gctaatagga gtggtatatt tagctattca cagccaactt catatggaat ttcctttcca     1620

aaaactatgg atgcagatga atcattaaca tctcgttcat ttgcacttgc tacacttgct     1680

acaccgctaa cctttagaag gcaagaagaa ttaaatctac aaataccatc aggtacttat     1740

atagatcgaa ttgagtttgt tccagtcgat gaaaccttta caacagaatc tgatctggat     1800

agagcacaac aggcggtgaa tgcgctgttt acttcttcca atcaaatcgg cttaaaaaca     1860

gatgtgacgg attatcatat tgatcaagta tccaatttag tggattgttt atcggatgaa     1920

ttttgtctgg atgaaaagcg agaattgtcc gagaaagtca aacatgcgaa gcgactcagt     1980

gatgagcgga atttacttca agatccaaac tttagaggaa tcaatagaca accagaccgt     2040

ggttggagag gaagtacaga tattaccatc caaggaggag atgacgtatt caaagagaat     2100

tacgttacac taccaggtac ctttgatgag tgctatccaa cgtatttata tcaaaaaata     2160

gatgagtcga aattaaaagc ctatacccgt tatcaattaa gagggtatat cggggatagt     2220

caagacttag aaatctattt aattcgttac aatgcaaaac acgaaatagt aaatgtacca     2280

ggtacaggga gtttatggac tctttctgta gaaaattcaa ttggaccttg tggagaaccg     2340

aatcgatgcg cgccacacct tgaatggaat cctaatctag agtgttcttg cagagaaggg     2400

gaaaaatgtg cccatcattc ccatcatttc tccttggaca ttgatgttgg atgtacagac     2460

ttaaatgagg acttaggtgt atgggcgata ttcaagatta agacgcaaga tggccatgca     2520

agactaggaa atctagagtt tctcgaagag aaaccactat taggggaagc actagctcgt     2580

gtgaaaagag cggagaagaa atggagagac aaacgcgaaa aattggaatg ggaaacaaat     2640

attgtttata aagaggcaaa agaatctgta gatgctttat ttgtgaactc tcaatatgat     2700

agattacaag cggatacgaa tatcgcgatg attcatgcgg cagataaacg cgttcataga     2760

attagagaag cataccttcc agaattatct gtaattccgg gtgtaaatgc gggcattttc     2820

gaagaattag agggacgcat tttcacagcc tactctctat atgatgcgag aaatgtcatt     2880

aaaaatggcg atttcaataa tggtttattg tgctggaact tgaaagggca tgtagatgta     2940

gaagaacaaa acaaccatcg ttcagtcctt gttgtcccgg aatgggaagc agaggtgtcc     3000

caagaagttc gtgtctgtcc aggtcgtggc tatatccttc gtgttacagc gtacaaagag     3060

ggatatggag agggctgcgt aaccatccat gagatcgaag acaatacaga cgaactgaaa     3120

ttcagcaact gtgtagaaga ggaagtatat ccaaacaaca cggtaacgtg taatgattat     3180

actgcgactc aagaagaata tgagggtacg tacacttctc gtaatcgagg atatgacgga     3240

gcctatgaaa gcaattcttc tgtaccagct gattatgcat cagcctatga agaaaaagcg     3300

tatacagatg gaagaagaga gaatccttgt gaatctaata gaggatatgg ggattacgcg     3360

ccactaccag ctggttatgt gacaaaggaa ttagagtact tcccagaaac cgataaggta     3420

tggattgaga tcggagaaac ggaaggaaca ttcattgtgg atagtgtgga attactcctt     3480

atggaggaat aa                                                         3492


<210>  4
<211>  1163
<212>  PRT
<213>  Bacillus thuringiensis

<400>  4

Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Ser Glu 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp 
    50                  55                  60                  


Gly Ser Ile Gly Arg Ser Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly 
305                 310                 315                 320 


Gly His Arg Val Asn Phe Thr Arg Ser Gly Val Thr Thr Phe Ile Gln 
                325                 330                 335     


Ser Pro Leu Tyr Gly Arg Glu Gly Asn Ala Glu Arg Ser Val Ile Ile 
            340                 345                 350         


Ser Ala Ser Ser Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr 
        355                 360                 365             


Gly Leu Asp Asn Ala Asn Pro Val Ala Gly Ile Glu Gly Val Glu Phe 
    370                 375                 380                 


Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp 
385                 390                 395                 400 


Ser Phe Asn Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ser Ile 
                405                 410                 415     


Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile Ser 
            420                 425                 430         


Gly Pro Arg Ile Ala Gly Val Val Phe Ser Trp Thr His Arg Ser Ala 
        435                 440                 445             


Ser Pro Thr Asn Glu Val Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp 
    450                 455                 460                 


Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly Pro 
465                 470                 475                 480 


Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu 
                485                 490                 495     


Gly Thr Leu Arg Val Thr Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr 
            500                 505                 510         


Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Ile Phe Ser 
        515                 520                 525             


Tyr Ser Gln Pro Thr Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp 
    530                 535                 540                 


Ala Asp Glu Ser Leu Thr Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala 
545                 550                 555                 560 


Thr Pro Leu Thr Phe Arg Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro 
                565                 570                 575     


Ser Gly Thr Tyr Ile Asp Arg Ile Glu Phe Val Pro Val Asp Glu Thr 
            580                 585                 590         


Phe Thr Thr Glu Ser Asp Leu Asp Arg Ala Gln Gln Ala Val Asn Ala 
        595                 600                 605             


Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp 
    610                 615                 620                 


Tyr His Ile Asp Gln Val Ser Asn Leu Val Asp Cys Leu Ser Asp Glu 
625                 630                 635                 640 


Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala 
                645                 650                 655     


Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg 
            660                 665                 670         


Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile 
        675                 680                 685             


Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu 
    690                 695                 700                 


Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile 
705                 710                 715                 720 


Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr 
                725                 730                 735     


Ile Gly Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala 
            740                 745                 750         


Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp Thr Leu 
        755                 760                 765             


Ser Val Glu Asn Ser Ile Gly Pro Cys Gly Glu Pro Asn Arg Cys Ala 
    770                 775                 780                 


Pro His Leu Glu Trp Asn Pro Asn Leu Glu Cys Ser Cys Arg Glu Gly 
785                 790                 795                 800 


Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val 
                805                 810                 815     


Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Ala Ile Phe Lys 
            820                 825                 830         


Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu 
        835                 840                 845             


Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys Arg Ala 
    850                 855                 860                 


Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn 
865                 870                 875                 880 


Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn 
                885                 890                 895     


Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His 
            900                 905                 910         


Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu Pro Glu 
        915                 920                 925             


Leu Ser Val Ile Pro Gly Val Asn Ala Gly Ile Phe Glu Glu Leu Glu 
    930                 935                 940                 


Gly Arg Ile Phe Thr Ala Tyr Ser Leu Tyr Asp Ala Arg Asn Val Ile 
945                 950                 955                 960 


Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Leu Lys Gly 
                965                 970                 975     


His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser Val Leu Val Val 
            980                 985                 990         


Pro Glu Trp Glu Ala Glu Val Ser  Gln Glu Val Arg Val  Cys Pro Gly 
        995                 1000                 1005             


Arg Gly  Tyr Ile Leu Arg Val  Thr Ala Tyr Lys Glu  Gly Tyr Gly 
    1010                 1015                 1020             


Glu Gly  Cys Val Thr Ile His  Glu Ile Glu Asp Asn  Thr Asp Glu 
    1025                 1030                 1035             


Leu Lys  Phe Ser Asn Cys Val  Glu Glu Glu Val Tyr  Pro Asn Asn 
    1040                 1045                 1050             


Thr Val  Thr Cys Asn Asp Tyr  Thr Ala Thr Gln Glu  Glu Tyr Glu 
    1055                 1060                 1065             


Gly Thr  Tyr Thr Ser Arg Asn  Arg Gly Tyr Asp Gly  Ala Tyr Glu 
    1070                 1075                 1080             


Ser Asn  Ser Ser Val Pro Ala  Asp Tyr Ala Ser Ala  Tyr Glu Glu 
    1085                 1090                 1095             


Lys Ala  Tyr Thr Asp Gly Arg  Arg Glu Asn Pro Cys  Glu Ser Asn 
    1100                 1105                 1110             


Arg Gly  Tyr Gly Asp Tyr Ala  Pro Leu Pro Ala Gly  Tyr Val Thr 
    1115                 1120                 1125             


Lys Glu  Leu Glu Tyr Phe Pro  Glu Thr Asp Lys Val  Trp Ile Glu 
    1130                 1135                 1140             


Ile Gly  Glu Thr Glu Gly Thr  Phe Ile Val Asp Ser  Val Glu Leu 
    1145                 1150                 1155             


Leu Leu  Met Glu Glu 
    1160             


<210>  5
<211>  2259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  5
atggaaggca acaacctgaa ccagtgcatt ccgtataact gcctgagcaa cccgaaagat       60

attattctgg gcgatgaacg tctggaaacc ggcaacaccg tggcggatat taccctgggc      120

attgtgaacc tgctgtttag cgaatttgtg ccgggcggcg gctttattct gggcctgctg      180

gatctgattt ggggcagcat tggccgtagc cagtgggatc tgtttctgga acagattgaa      240

cagctgatta aacagcgtat tgaagaattt gcgcgtaacc aggcgattag ccgtctggaa      300

ggcctgagcg atctgtataa aacctatgcg cgtgcgttta gcgattggga agcggatccg      360

accaacccgg cgctgcgtga agaaatgcgt attcagttta acgatatgaa cagcgcgatt      420

attaccgcgc tgccgctgtt tcgtgtgcag aactatgaag tggcgctgct gagcgtgtat      480

gtgcaggcgg cgaacctgca tctgagcatt ctgcgtgatg tgagcgtgtt tggcgaacgt      540

tggggctatg ataccgcgac cattaacaac cgttatagcg atctgaccag cctgattcat      600

gtgtatacca accattgcgt ggatacctat aaccagggcc tgcgtcgtct ggaaggccgt      660

tttctgaccg attggattgt gtataaccgt tttcgtcgtc agctgaccat tagcgtgctg      720

gatattgtgg cgttttttcc gaactatgat attcgtacct atccgattca gaccgcgacc      780

cagctgaccc gtgaagtgta tctggatctg ccgtttatta acgaaaacct gagcccggcg      840

gcgagctatc cgacctttag cgcggcggaa agcgcgatta ttcgtagccc gcatctggtg      900

gattttctga acagctttac catttatacc gatagcctgg cgcgttatgc gtattggggc      960

ggccatctgg tgaacagctt tcgtaccggc accaccacca acctgattcg tagcccgctg     1020

tatggccgtg aaggcaacac cgaacgtccg gtgaccatta ccgcgagccc gagcgtgccg     1080

atttttcgta ccctgagcta tattaccggc ctggataaca gcaacccggt ggcgggcatt     1140

gaaggcgtgg aatttcagaa caccattagc cgtagcattt atcgtaaaag cggcccgatt     1200

gatagcttta gcgaactgcc gccgcaggat gcgagcgtga gcccggcgat tggctatagc     1260

catcgtctgt gccatgcgac ctttctggaa cgtattagcg gcccgcgtat tgcgggcacc     1320

gtgtttagct ggacccatcg tagcgcgagc ccgaccaacg aagtgagccc gagccgtatt     1380

acccagattc cgtgggtgaa agcgcatacc ctggcgagcg gcgcgagcgt gattaaaggc     1440

ccgggcttta ccggcggcga tattctgacc cgtaacagca tgggcgaact gggcaccctg     1500

cgtgtgacct ttaccggccg tctgccgcag agctattata ttcgttttcg ttatgcgagc     1560

gtggcgaacc gtagcggcac ctttcgttat agccagccgc cgagctatgg cattagcttt     1620

ccgaaaacca tggatgcggg cgaaccgctg accagccgta gctttgcgca taccaccctg     1680

tttaccccga ttacctttag ccgtgcgcag gaagaatttg atctgtatat tcagagcggc     1740

gtgtatattg atcgtattga atttattccg gtgaccgcga cctttgaagc ggaatatgat     1800

ctggaacgtg cgcagaaagt ggtgaacgcg ctgtttacca gcaccaacca gctgggcctg     1860

aaaaccgatg tgaccgatta tcatattgat caggtgagca acctggtggc gtgcctgagc     1920

gatgaatttt gcctggatga aaaacgtgaa ctgagcgaaa aagtgaaaca tgcgaaacgt     1980

ctgagcgatg aacgtaacct gctgcaggat ccgaactttc gtggcattaa ccgtcagccg     2040

gatcgtggct ggcgtggcag caccgatatt accattcagg gcggcgatga tgtgtttaaa     2100

gaaaactatg tgaccctgcc gggcaccttt gatgaatgct atccgaccta tctgtatcag     2160

aaaattgatg aaagcaaact gaaagcgtat acccgttatc agctgcgtgg ctatattgaa     2220

gatagccagg atctggaaat ttatctgatt cgttataac                            2259


<210>  6
<211>  753
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  6

Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Ser Glu 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp 
    50                  55                  60                  


Gly Ser Ile Gly Arg Ser Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn 
    


<210>  7
<211>  2259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  7
atggaaggca acaacctgaa ccagtgcatt ccgtataact gcctgaccaa cccgaaagat       60

attattctgg gcgatgaacg tctggaaacc ggcaacaccg tggcggatat taccctgggc      120

attgtgaacc tgctgtttac cgaatttgtg ccgggcggcg gctttattct gggcctgctg      180

gatgtgattt ggggcagcat tggccgtagc cagtgggaac tgtttctgga acagattgaa      240

cagctgatta aacagcgtat tgaagaattt gcgcgtaacc aggcgattag ccgtctggaa      300

ggcctgagcg atctgtataa aacctatgcg cgtgcgttta gcgattggga agcggatccg      360

accaacccgg cgctgcgtga agaaatgcgt attcagttta acgatatgaa cagcgcgatt      420

attaccgcgc tgccgctgtt tcgtgtgcag aactatgaag tggcgctgct gagcgtgtat      480

gtgcaggcgg cgaacctgca tctgagcatt ctgcgtgatg tgagcgtgtt tggcgaacgt      540

tggggctatg ataccgcgac cattaacaac cgttatagcg atctgaccag cctgattcat      600

gtgtatacca accattgcgt ggatacctat aaccagggcc tgcgtcgtct ggaaggccgt      660

tttctgaccg attggattgt gtataaccgt tttcgtcgtc agctgaccat tagcgtgctg      720

gatattgtgg cgttttttcc gaactatgat attcgtacct atccgattca gaccgcgacc      780

cagctgaccc gtgaagtgta tctggatctg ccgtttatta acgaaaacct gagcccggcg      840

gcgagctatc cgacctttag cgcggcggaa agcgcgatta ttcgtagccc gcatctggtg      900

gattttctga acagctttac catttatacc gatagcctgg cgcgttatgc gtattggggc      960

ggccatctgg tgaacagctt tcgtaccggc accaccacca acctgattcg tagcccgctg     1020

tatggccgtg aaggcaacac cgaacgtccg gtgaccatta ccgcgagccc gagcgtgccg     1080

atttttcgta ccctgagcta tattaccggc ctggataaca gcaacccggt ggcgggcatt     1140

gaaggcgtgg aatttcagaa caccattagc cgtagcattt atcgtaaaag cggcccgatt     1200

gatagcttta gcgaactgcc gccgcaggat gcgagcgtga gcccggcgat tggctatagc     1260

catcgtctgt gccatgcgac ctttctggaa cgtattagcg gcccgcgtat tgcgggcacc     1320

gtgtttagct ggacccatcg tagcgcgagc ccgaccaacg aagtgagccc gagccgtatt     1380

acccagattc cgtgggtgaa agcgcatacc ctggcgagcg gcgcgagcgt gattaaaggc     1440

ccgggcttta ccggcggcga tattctgacc cgtaacagca tgggcgaact gggcaccctg     1500

cgtgtgagct ttaccggccg tctgccgcag agctattata ttcgttttcg ttatgcgagc     1560

gtggcgaacc gtaccggcac ctttcgttat agccagccgc cgagctatgg cctgagcttt     1620

ccgaaaacca tggatgcggg cgaaccgctg accagccgta gctttgcgca taccaccctg     1680

tttaccccga ttacctttag ccgtgcgcag gaagaatttg atctgtatat tcagagcggc     1740

gtgtatattg atcgtattga atttattccg gtgaccgcga cctttgaagc ggaatatgat     1800

ctggaacgtg cgcagaaagt ggtgaacgcg ctgtttacca gcaccaacca gctgggcctg     1860

aaaaccgatg tgaccgatta tcatattgat caggtgagca acctggtggc gtgcctgagc     1920

gatgaatttt gcctggatga aaaacgtgaa ctgagcgaaa aagtgaaaca tgcgaaacgt     1980

ctgagcgatg aacgtaacct gctgcaggat ccgaactttc gtggcattaa ccgtcagccg     2040

gatcgtggct ggcgtggcag caccgatatt accattcagg gcggcgatga tgtgtttaaa     2100

gaaaactatg tgaccctgcc gggcaccttt gatgaatgct atccgaccta tctgtatcag     2160

aaaattgatg aaagcaaact gaaagcgtat acccgttatc agctgcgtgg ctatattgaa     2220

gatagccagg atctggaaat ttatctgatt cgttataac                            2259


<210>  8
<211>  753
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  8

Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Thr 
1               5                   10                  15      


Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Thr Glu 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Val Ile Trp 
    50                  55                  60                  


Gly Ser Ile Gly Arg Ser Gln Trp Glu Leu Phe Leu Glu Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Ser Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Thr Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Leu Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn 
    


<210>  9
<211>  2259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  9
atggaaggca acaacctgaa ccagtgcatt ccgtataact gcctgaccaa cccgaaagat       60

attattctgg gcgatgaacg tctggaaacc ggcaacaccg tggcggatat taccctgggc      120

attgtgaacc tgctgtttac cgaatttgtg ccgggcggcg gctttattct gggcctgctg      180

gatgtgattt ggggcagcat tggccgtagc cagtgggaac tgtttctgga acagattgaa      240

cagctgatta aacagcgtat tgatgatttt gcgcgtaacc aggcgattag ccgtctggaa      300

ggcctgagcg atctgtataa aacctatgcg cgtgcgttta gcgattggga agcggatccg      360

accaacccgg cgctgcgtga agaaatgcgt attcagttta acgatatgaa cagcgcgatt      420

attaccgcgc tgccgctgtt tcgtgtgcag aactatgaag tggcgctgct gagcgtgtat      480

gtgcaggcgg cgaacctgca tctgagcatt ctgcgtgatg tgagcgtgtt tggcgaacgt      540

tggggctatg ataccgcgac cattaacaac cgttatagcg atctgaccag cctgattcat      600

gtgtatacca accattgcgt ggatacctat aaccagggcc tgcgtcgtct ggaaggccgt      660

tttctgaccg attggattgt gtataaccgt tttcgtcgtc agctgaccat tagcgtgctg      720

gatattgtgg cgttttttcc gaactatgat attcgtacct atccgattca gaccgcgacc      780

cagctgaccc gtgaagtgta tctggatctg ccgtttatta acgaaaacct gagcccggcg      840

gcgagctatc cgacctttag cgcggcggaa agcgcgatta ttcgtagccc gcatctggtg      900

gattttatta acagctttac catttatacc gatagcctgg cgcgttatgc gtattggggc      960

ggccatctgg tgaacagctt tcgtaccggc accaccacca acctgattcg tagcccgctg     1020

tatggccgtg aaggcaacac cgaacgtccg gtgaccatta ccgcgagccc gagcgtgccg     1080

atttttcgta ccctgagcta tattaccggc ctggataaca gcaacccggt ggcgggcatt     1140

gaaggcgtgg aatttcagaa caccattagc cgtagcattt atcgtaaaag cggcccgatt     1200

gatagcttta gcgaactgcc gccgcaggat gcgagcgtga gcccggcgat tggctatagc     1260

catcgtctgt gccatgcgac ctttctggaa cgtattagcg gcccgcgtat tgcgggcacc     1320

gtgtttagct ggacccatcg tagcgcgagc ccgaccaacg aagtgagccc gagccgtatt     1380

acccagattc cgtgggtgaa agcgcatacc ctggcgagcg gcgcgagcgt gattaaaggc     1440

ccgggcttta ccggcggcga tattctgacc cgtaacagca tgggcgaact gggcaccctg     1500

cgtgtgagct ttaccggccg tctgccgcag agctattata ttcgttttcg ttatgcgagc     1560

gtggcgaacc gtaccggcac ctttcgttat agccagccgc cgagctatgg cctgagcttt     1620

ccgaaaacca tggatgcggg cgaaccgctg accagccgta gctttgcgca taccaccctg     1680

tttaccccga ttacctttag ccgtgcgcag gaagaatttg atctgtatat tcagagcggc     1740

gtgtatattg atcgtattga atttattccg gtgaccgcga cctttgaagc ggaatatgat     1800

ctggaacgtg cgcagaaagt ggtgaacgcg ctgtttacca gcaccaacca gctgggcctg     1860

aaaaccgatg tgaccgatta tcatattgat caggtgagca acctggtggc gtgcctgagc     1920

gatgaatttt gcctggatga aaaacgtgaa ctgagcgaaa aagtgaaaca tgcgaaacgt     1980

ctgagcgatg aacgtaacct gctgcaggat ccgaactttc gtggcattaa ccgtcagccg     2040

gatcgtggct ggcgtggcag caccgatatt accattcagg gcggcgatga tgtgtttaaa     2100

gaaaactatg tgaccctgcc gggcaccttt gatgaatgct atccgaccta tctgtatcag     2160

aaaattgatg aaagcaaact gaaagcgtat acccgttatc agctgcgtgg ctatattgaa     2220

gatagccagg atctggaaat ttatctgatt cgttataac                            2259


<210>  10
<211>  753
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of MP589FL

<400>  10

Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Thr 
1               5                   10                  15      


Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Thr Glu 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Val Ile Trp 
    50                  55                  60                  


Gly Ser Ile Gly Arg Ser Gln Trp Glu Leu Phe Leu Glu Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Lys Gln Arg Ile Asp Asp Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Ile Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Ser Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Thr Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Leu Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn 
    


