                         SEQUENCE LISTING

<110>  CREUSOT, Remi
 
<120>  TANDEM EPITOPE CONSTRUCTS FOR PRESENTATION OF CD4 AND CD8 
       EPITOPES AND USES THEREOF

<130>  15003-324PC0

<150>  US 62/062,325
<151>  2014-10-10

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1

Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly 
1               5                   10                  15  


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2

Leu Tyr Leu Val Cys Gly Glu Arg Gly 
1               5                   


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3

Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Glu Gly 
1               5                   10                  15  


<210>  4
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Trp Ser Arg Met Asp Gln Leu Ala Lys Glu Leu Thr Ala Glu 
1               5                   10                  


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5

Ala Val Pro Pro Leu Trp Val Arg Met Glu 
1               5                   10  


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6

Val Tyr Leu Lys Thr Asn Val Phe Leu 
1               5                   


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7

Lys Lys Gly Ala Ala Ala Leu Gly Ile Gly Thr Asp Ser Val Ile 
1               5                   10                  15  


<210>  8
<211>  675
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
actagtgcca ccatgatgga tcaagctaga tcagcattct ctaacttgtt tggtggagaa       60

ccattgtcat atacccggtt cagcctggct cggcaagtag atggcgataa cagtcatgtg      120

gagatgaaac ttgctgtaga tgaagaagaa aatgctgaca ataacacaaa ggccaatgtc      180

acaaaaccaa aaaggtgtag tggaagtatc tgctatggga ctattgctgt gatcgtcttt      240

ttcttgattg gatttatgat tggctacttg ggctattgta aaggggtaga accaaaaact      300

gagtgtgaga gactggcagg aaccgagtct ccagtgaggg aggagccagg agaggacttc      360

cctgcaccgc ggtgtggttc ccacctggtg gaggctctct acctggtgtg tggggaggag      420

ggcttcttca agcgagcagt tcgacctcta tgggtacgta tggaaaagcg gtctctcaag      480

aagggagctg cagccttagg gattggaaca gacagtgtga ttctgattga gggcagagga      540

agtctgctaa catgcggtga cgtcgaggag aatcctggac ctgaggctct ctacctggtg      600

tgtggggagc gtggcttctt cttgagtgtg tacctgaaga ccaacgtctt cctcttcctg      660

cccgggtagg tcgac                                                       675


<210>  9
<211>  675
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
actagtgcca ccatgatgga ccaagctaga tccgccttca gcaatctgtt cggaggagag       60

cccctctcct atacaagatt ctccctggcc aggcaagtgg acggcgacaa ctcccacgtc      120

gagatgaaac tcgccgtgga tgaagaggag aacgccgaca ataacaccaa ggccaacgtg      180

accaagccta agaggtgcag cggaagcatc tgctacggca caatcgccgt gatcgtcttc      240

ttcctgatcg gattcatgat cggatacctg ggctactgca agggcgtgga gcctaaaacc      300

gagtgcgaga gactcgctgg aacagagtcc cctgtcaggg aggaacctgg agaggatttc      360

cctgccccgc ggtgcggatc ccatctggtc gaagccctgt acctggtctg tggcgaggaa      420

ggattcttca agagggctgt caggcctctg tgggtgagga tggaaaagag atccctgaaa      480

aaaggcgccg ctgccctggg aattggcacc gactccgtca ttctcatcga gggcagagga      540

tccctcctga cctgtggcga cgtggaggaa aaccccggac ccgaagctct gtacctggtg      600

tgtggcgaaa ggggcttttt cctgtccgtc tacctgaaaa ccaatgtctt tctgtttctg      660

cccgggtagg tcgac                                                       675


<210>  10
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10

Met Met Asp Gln Ala Arg Ser Ala Phe Ser Asn Leu Phe Gly Gly Glu 
1               5                   10                  15      


Pro Leu Ser Tyr Thr Arg Phe Ser Leu Ala Arg Gln Val Asp Gly Asp 
            20                  25                  30          


Asn Ser His Val Glu Met Lys Leu Ala Val Asp Glu Glu Glu Asn Ala 
        35                  40                  45              


Asp Asn Asn Thr Lys Ala Asn Val Thr Lys Pro Lys Arg Cys Ser Gly 
    50                  55                  60                  


Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile Val Phe Phe Leu Ile Gly 
65                  70                  75                  80  


Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys Gly Val Glu Pro Lys Thr 
                85                  90                  95      


Glu Cys Glu Arg Leu Ala Gly Thr Glu Ser Pro Val Arg Glu Glu Pro 
            100                 105                 110         


Gly Glu Asp Phe Pro Ala Pro Arg Cys Gly Ser His Leu Val Glu Ala 
        115                 120                 125             


Leu Tyr Leu Val Cys Gly Glu Glu Gly Phe Phe Lys Arg Ala Val Arg 
    130                 135                 140                 


Pro Leu Trp Val Arg Met Glu Lys Arg Ser Leu Lys Lys Gly Ala Ala 
145                 150                 155                 160 


Ala Leu Gly Ile Gly Thr Asp Ser Val Ile Leu Ile Glu Gly Arg Gly 
                165                 170                 175     


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Glu Ala 
            180                 185                 190         


Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Leu Ser Val Tyr Leu 
        195                 200                 205             


Lys Thr Asn Val Phe Leu Phe Leu Pro Gly 
    210                 215             


<210>  11
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatat       60

acccggttca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt      120

gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa      180

aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga      240

tttatgattg gctacttggg ctattgtaaa ggggtagaac caaaaactga gtgtgagaga      300

ctggcaggaa ccgagtctcc agtgagggag gagccaggag aggacttccc tgca            354


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12

Met Met Asp Gln Ala Arg Ser Ala Phe Ser Asn Leu Phe Gly Gly Glu 
1               5                   10                  15      


Pro Leu Ser Tyr Thr Arg Phe Ser Leu Ala Arg Gln Val Asp Gly Asp 
            20                  25                  30          


Asn Ser His Val Glu Met Lys Leu Ala Val Asp Glu Glu Glu Asn Ala 
        35                  40                  45              


Asp Asn Asn Thr Lys Ala Asn Val Thr Lys Pro Lys Arg Cys Ser Gly 
    50                  55                  60                  


Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile Val Phe Phe Leu Ile Gly 
65                  70                  75                  80  


Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys Gly Val Glu Pro Lys Thr 
                85                  90                  95      


Glu Cys Glu Arg Leu Ala Gly Thr Glu Ser Pro Val Arg Glu Glu Pro 
            100                 105                 110         


Gly Glu Asp Phe Pro Ala 
        115             


<210>  13
<211>  240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
atggatgacc aacgcgacct catctctaac catgaacagt tgcccatact gggcaaccgc       60

cctagagagc cagaaaggtg cagccgtgga gctctgtaca ccggtgtctc tgtcctggtg      120

gctctgctct tggctgggca ggccaccact gcttacttcc tgtaccagca acagggccgc      180

ctagacaagc tgaccatcac ctcccagaac ctgcaactgg agagccttcg catgaagctt      240


<210>  14
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn His Glu Gln Leu Pro Ile 
1               5                   10                  15      


Leu Gly Asn Arg Pro Arg Glu Pro Glu Arg Cys Ser Arg Gly Ala Leu 
            20                  25                  30          


Tyr Thr Gly Val Ser Val Leu Val Ala Leu Leu Leu Ala Gly Gln Ala 
        35                  40                  45              


Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys Leu 
    50                  55                  60                  


Thr Ile Thr Ser Gln Asn Leu Gln Leu Glu Ser Leu Arg Met Lys Leu 
65                  70                  75                  80  


<210>  15
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
atggatgacc aacgcgacct catctctaac catgaacagt tgcccatact gggcaaccgc       60

cctagagagc cagaaaggtg cagccgtgga gctctgtaca ccggtgtctc tgtcctggtg      120

gctctgctct tggctgggca ggccaccact gcttacttcc tgtaccagca acagggccgc      180

ctagacaagc tgaccatcac ctcccagaac ctgcaactgg agagccttcg catgaagctt      240

ccgaaatctg ccaaacctgt gagccagatg cggatggcta ctcccttgct gatgcgtcca      300

atgtccatgg ataacatgct ccttgggcct gtgaagaacg ttaccaagta cggcaacatg      360

acccaggacc atgtgatgca tctgctcacg aggtctggac ccctggagta cccgcagctg      420

aaggggacct tcccagagaa tctgaagcat cttaagaact ccatggatgg cgtgaactgg      480

aagatcttcg agagctggat gaagcagtgg ctcttgtttg agatgagcaa gaactccctg      540

gaggagaaga agcccaccga ggctccacct aaagagccac tggacatgga agacctatct      600

tctggcctgg gagtgaccag gcaggaactg ggtcaagtca cc                         642


<210>  16
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn His Glu Gln Leu Pro Ile 
1               5                   10                  15      


Leu Gly Asn Arg Pro Arg Glu Pro Glu Arg Cys Ser Arg Gly Ala Leu 
            20                  25                  30          


Tyr Thr Gly Val Ser Val Leu Val Ala Leu Leu Leu Ala Gly Gln Ala 
        35                  40                  45              


Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys Leu 
    50                  55                  60                  


Thr Ile Thr Ser Gln Asn Leu Gln Leu Glu Ser Leu Arg Met Lys Leu 
65                  70                  75                  80  


Pro Lys Ser Ala Lys Pro Val Ser Gln Met Arg Met Ala Thr Pro Leu 
                85                  90                  95      


Leu Met Arg Pro Met Ser Met Asp Asn Met Leu Leu Gly Pro Val Lys 
            100                 105                 110         


Asn Val Thr Lys Tyr Gly Asn Met Thr Gln Asp His Val Met His Leu 
        115                 120                 125             


Leu Thr Arg Ser Gly Pro Leu Glu Tyr Pro Gln Leu Lys Gly Thr Phe 
    130                 135                 140                 


Pro Glu Asn Leu Lys His Leu Lys Asn Ser Met Asp Gly Val Asn Trp 
145                 150                 155                 160 


Lys Ile Phe Glu Ser Trp Met Lys Gln Trp Leu Leu Phe Glu Met Ser 
                165                 170                 175     


Lys Asn Ser Leu Glu Glu Lys Lys Pro Thr Glu Ala Pro Pro Lys Glu 
            180                 185                 190         


Pro Leu Asp Met Glu Asp Leu Ser Ser Gly Leu Gly Val Thr Arg Gln 
        195                 200                 205             


Glu Leu Gly Gln Val Thr 
    210                 


<210>  17
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
atgactttgg acacccagat gtttcagaat gcacactctg gaagccaatg gctacttcca       60

ccactgaca                                                               69


<210>  18
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18

Met Thr Leu Asp Thr Gln Met Phe Gln Asn Ala His Ser Gly Ser Gln 
1               5                   10                  15      


Trp Leu Leu Pro Pro Leu Thr 
            20              


<210>  19
<211>  651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19
gatgccacta tccaggccta cctgtcgagt ggcaacttca gcaaggaaga gacacactgc       60

acacaggatg gaccttcccc aaccactggg ccacccagcc cctcaccacc acttgtgccc      120

acaaacccca ctgtatccaa gtacaatgtt actggtaaca acggaacctg cctgctggcc      180

tctatggcac tgcaactgaa tatcacctac ctgaaaaagg acaacaagac ggtgaccaga      240

gcgttcaaca tcagcccaaa tgacacatct agtgggagtt gcggtatcaa cttggtgacc      300

ctgaaagtgg agaacaagaa cagagccctg gaattgcagt ttgggatgaa tgccagctct      360

agcctgtttt tcctgcaagg agtgcgcttg aatatgactc ttcctgatgc cctagtgccc      420

acattcagca tctccaacca ttcactgaaa gctcttcagg ccactgtggg aaactcatac      480

aagtgcaaca ctgaggaaca catctttgtc agcaagatgc tctccctcaa tgtcttcagt      540

gtgcaggtcc aggctttcaa ggtggacagt gacaggtttg ggtctgtgga agagtgtgtt      600

caggatggta acaacatgtt gatccccatt gctgtgggcg gtgccctggc a               651


<210>  20
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20

Asp Ala Thr Ile Gln Ala Tyr Leu Ser Ser Gly Asn Phe Ser Lys Glu 
1               5                   10                  15      


Glu Thr His Cys Thr Gln Asp Gly Pro Ser Pro Thr Thr Gly Pro Pro 
            20                  25                  30          


Ser Pro Ser Pro Pro Leu Val Pro Thr Asn Pro Thr Val Ser Lys Tyr 
        35                  40                  45              


Asn Val Thr Gly Asn Asn Gly Thr Cys Leu Leu Ala Ser Met Ala Leu 
    50                  55                  60                  


Gln Leu Asn Ile Thr Tyr Leu Lys Lys Asp Asn Lys Thr Val Thr Arg 
65                  70                  75                  80  


Ala Phe Asn Ile Ser Pro Asn Asp Thr Ser Ser Gly Ser Cys Gly Ile 
                85                  90                  95      


Asn Leu Val Thr Leu Lys Val Glu Asn Lys Asn Arg Ala Leu Glu Leu 
            100                 105                 110         


Gln Phe Gly Met Asn Ala Ser Ser Ser Leu Phe Phe Leu Gln Gly Val 
        115                 120                 125             


Arg Leu Asn Met Thr Leu Pro Asp Ala Leu Val Pro Thr Phe Ser Ile 
    130                 135                 140                 


Ser Asn His Ser Leu Lys Ala Leu Gln Ala Thr Val Gly Asn Ser Tyr 
145                 150                 155                 160 


Lys Cys Asn Thr Glu Glu His Ile Phe Val Ser Lys Met Leu Ser Leu 
                165                 170                 175     


Asn Val Phe Ser Val Gln Val Gln Ala Phe Lys Val Asp Ser Asp Arg 
            180                 185                 190         


Phe Gly Ser Val Glu Glu Cys Val Gln Asp Gly Asn Asn Met Leu Ile 
        195                 200                 205             


Pro Ile Ala Val Gly Gly Ala Leu Ala 
    210                 215         


<210>  21
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21
tgtggttccc acctggtgga ggctctctac ctggtgtgtg gggagcgtgg cttcttc          57


<210>  22
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22

Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg 
1               5                   10                  15      


Gly Phe Phe 
            


<210>  23
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
tgtggttccc acctggtgga ggctctctac ctggtgtgtg gggaggaggg cttcttc          57


<210>  24
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  24

Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Glu 
1               5                   10                  15      


Gly Phe Phe 
            


<210>  25
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  25
tgtggttccc acctggtgga ggctctctac ctggtgtgtg ggggagaggg cttcttc          57


<210>  26
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  26

Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Gly Glu 
1               5                   10                  15      


Gly Phe Phe 
            


<210>  27
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27
aagcgatgga gcaggatgga ccagctggcc aaagagctga cagcagagaa gcgg             54


<210>  28
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28

Lys Arg Trp Ser Arg Met Asp Gln Leu Ala Lys Glu Leu Thr Ala Glu 
1               5                   10                  15      


Lys Arg 
        


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29
aagcgagcag ttcgacctct atgggtacgt atggaaaagc gg                          42


<210>  30
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30

Lys Arg Ala Val Arg Pro Leu Trp Val Arg Met Glu Lys Arg 
1               5                   10                  


<210>  31
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31
tctctcaaga agggagctgc agccttaggg attggaacag acagtgtgat tctgatt          57


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32

Ser Leu Lys Lys Gly Ala Ala Ala Leu Gly Ile Gly Thr Asp Ser Val 
1               5                   10                  15      


Ile Leu Ile 
            


<210>  33
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33
agaatgagcc gcctctcaaa ggtggcgcca gtgattaaag ccagaatgat ggagtatggg       60

accacaatgg tc                                                           72


<210>  34
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34

Arg Met Ser Arg Leu Ser Lys Val Ala Pro Val Ile Lys Ala Arg Met 
1               5                   10                  15      


Met Glu Tyr Gly Thr Thr Met Val 
            20                  


<210>  35
<211>  57
<212>  DNA
<213>  Porcine teschovirus-1

<400>  35
gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacct          57


<210>  36
<211>  19
<212>  PRT
<213>  Porcine teschovirus-1

<400>  36

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  37
<211>  54
<212>  DNA
<213>  Thosea asigna virus

<400>  37
gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg acct             54


<210>  38
<211>  18
<212>  PRT
<213>  Thosea asigna virus

<400>  38

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  39
<211>  60
<212>  DNA
<213>  Equine rhinitis A virus

<400>  39
cagtgtacta attatgctct cttgaaattg gctggagatg ttgagagcaa ccctggacct       60


<210>  40
<211>  20
<212>  PRT
<213>  Equine rhinitis A virus

<400>  40

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  41
<211>  66
<212>  DNA
<213>  Foot-and-mouth disease virus

<400>  41
gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtgga gtccaaccct       60

ggacct                                                                  66


<210>  42
<211>  22
<212>  PRT
<213>  Foot-and-mouth disease virus

<400>  42

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  43
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  43
gaggctctct acctggtgtg tggggagcgt ggcttcttc                              39


<210>  44
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  44

Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe 
1               5                   10              


<210>  45
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  45
ttgagtgtgt acctgaagac caacgtcttc ctcttcctg                              39


<210>  46
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  46

Leu Ser Val Tyr Leu Lys Thr Asn Val Phe Leu Phe Leu 
1               5                   10              


