                         SEQUENCE LISTING

<110>  Lipotek Pty Ltd  AND The Australian National University
 
<120>  Chimeric proteins

<130>  35240593 WJP/TKU

<150>  2014904028
<151>  2015-10-09

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  509
<212>  PRT
<213>  HIV

<400>  1

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Asn Cys Thr Asp Leu Arg Asn Val Thr Asn Ile Asn Asn Ser Ser 
    130                 135                 140                 


Glu Gly Met Arg Gly Glu Ile Lys Asn Cys Ser Phe Asn Ile Thr Thr 
145                 150                 155                 160 


Ser Ile Arg Asp Lys Val Lys Lys Asp Tyr Ala Leu Phe Tyr Arg Leu 
                165                 170                 175     


Asp Val Val Pro Ile Asp Asn Asp Asn Thr Ser Tyr Arg Leu Ile Asn 
            180                 185                 190         


Cys Asn Thr Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Glu 
        195                 200                 205             


Pro Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Phe Ala Ile Leu Lys 
    210                 215                 220                 


Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys Lys Asn Val Ser 
225                 230                 235                 240 


Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu 
                245                 250                 255     


Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val Ile Arg Ser Ser 
            260                 265                 270         


Asn Phe Thr Asp Asn Ala Lys Asn Ile Ile Val Gln Leu Lys Glu Ser 
        275                 280                 285             


Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile 
    290                 295                 300                 


His Ile Gly Pro Gly Arg Ala Phe Tyr Thr Thr Gly Asp Ile Ile Gly 
305                 310                 315                 320 


Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Thr Lys Trp Asn Asn 
                325                 330                 335     


Thr Leu Asn Gln Ile Ala Thr Lys Leu Lys Glu Gln Phe Gly Asn Asn 
            340                 345                 350         


Lys Thr Ile Val Phe Asn Gln Ser Ser Gly Gly Asp Pro Glu Ile Val 
        355                 360                 365             


Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr 
    370                 375                 380                 


Gln Leu Phe Asn Ser Thr Trp Asn Phe Asn Gly Thr Trp Asn Leu Thr 
385                 390                 395                 400 


Gln Ser Asn Gly Thr Glu Gly Asn Asp Thr Ile Thr Leu Pro Cys Arg 
                405                 410                 415     


Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr 
            420                 425                 430         


Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly 
        435                 440                 445             


Leu Ile Leu Thr Arg Asp Gly Gly Asn Asn His Asn Asn Asp Thr Glu 
    450                 455                 460                 


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg 
            500                 505                 


<210>  2
<211>  1527
<212>  DNA
<213>  HIV

<400>  2
atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg       60

ctccttggga tattgatgat ctgtagtgct acagaaaaat tgtgggtcac agtctattat      120

ggggtacctg tgtggaaaga agcaaccacc actctatttt gtgcatcaga tgctaaagca      180

tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagtattgga aaatgtgaca gaaaatttta acatgtggaa aaataacatg      300

gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaaccc cactctgtgt tactttaaat tgcactgatt tgaggaatgt tactaatatc      420

aataatagta gtgagggaat gagaggagaa ataaaaaact gctctttcaa tatcaccaca      480

agcataagag ataaggtgaa gaaagactat gcactttttt atagacttga tgtagtacca      540

atagataatg ataatactag ctataggttg ataaattgta atacctcaac cattacacag      600

gcctgtccaa aggtatcctt tgagccaatt cccatacatt attgtacccc ggctggtttt      660

gcgattctaa agtgtaaaga taagaagttc aatggaacag ggccatgtaa aaatgtcagc      720

acagtacaat gtacacatgg aattaggcca gtagtgtcaa ctcaactgct gttaaatggc      780

agtctagcag aagaagaggt agtaattaga tctagtaatt tcacagacaa tgcaaaaaac      840

ataatagtac agttgaaaga atctgtagaa attaattgta caagacccaa caacaataca      900

aggaaaagta tacatatagg accaggaaga gcattttata caacaggaga cataatagga      960

gatataagac aagcacattg caacattagt agaacaaaat ggaataacac tttaaatcaa     1020

atagctacaa aattaaaaga acaatttggg aataataaaa caatagtctt taatcaatcc     1080

tcaggagggg acccagaaat tgtaatgcac agttttaatt gtggagggga atttttctac     1140

tgtaattcaa cacaactgtt taatagtact tggaatttta atggtacttg gaatttaaca     1200

caatcgaatg gtactgaagg aaatgacact atcacactcc catgtagaat aaaacaaatt     1260

ataaacatgt ggcaagaagt aggaaaagca atgtatgccc ctcccatcag aggacaaatt     1320

agatgttcat caaatattac agggctgata ttaacaagag atggtggaaa taaccacaat     1380

aatgataccg agacctttag acctggagga ggagatatga gggacaattg gagaagtgaa     1440

ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag     1500

agaagagtgg tgcagagaga aaaaaga                                         1527


<210>  3
<211>  495
<212>  PRT
<213>  Salmonella typhimurium

<400>  3

Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn 
1               5                   10                  15      


Asn Leu Asn Lys Ser Gln Ser Ala Leu Gly Thr Ala Ile Glu Arg Leu 
            20                  25                  30          


Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln 
        35                  40                  45              


Ala Ile Ala Asn Arg Phe Thr Ala Asn Ile Lys Gly Leu Thr Gln Ala 
    50                  55                  60                  


Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly 
65                  70                  75                  80  


Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ala 
                85                  90                  95      


Val Gln Ser Ala Asn Ser Thr Asn Ser Gln Ser Asp Leu Asp Ser Ile 
            100                 105                 110         


Gln Ala Glu Ile Thr Gln Arg Leu Asn Glu Ile Asp Arg Val Ser Gly 
        115                 120                 125             


Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ala Gln Asp Asn Thr Leu 
    130                 135                 140                 


Thr Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Asp Ile Asp Leu 
145                 150                 155                 160 


Lys Gln Ile Asn Ser Gln Thr Leu Gly Leu Asp Thr Leu Asn Val Gln 
                165                 170                 175     


Gln Lys Tyr Lys Val Ser Asp Thr Ala Ala Thr Val Thr Gly Tyr Ala 
            180                 185                 190         


Asp Thr Thr Ile Ala Leu Asp Asn Ser Thr Phe Lys Ala Ser Ala Thr 
        195                 200                 205             


Gly Leu Gly Gly Thr Asp Gln Lys Ile Asp Gly Asp Leu Lys Phe Asp 
    210                 215                 220                 


Asp Thr Thr Gly Lys Tyr Tyr Ala Lys Val Thr Val Thr Gly Gly Thr 
225                 230                 235                 240 


Gly Lys Asp Gly Tyr Tyr Glu Val Ser Val Asp Lys Thr Asn Gly Glu 
                245                 250                 255     


Val Thr Leu Ala Gly Gly Ala Thr Ser Pro Leu Thr Gly Gly Leu Pro 
            260                 265                 270         


Ala Thr Ala Thr Glu Asp Val Lys Asn Val Gln Val Ala Asn Ala Asp 
        275                 280                 285             


Leu Thr Glu Ala Lys Ala Ala Leu Thr Ala Ala Gly Val Thr Gly Thr 
    290                 295                 300                 


Ala Ser Val Val Lys Met Ser Tyr Thr Asp Asn Asn Gly Lys Thr Ile 
305                 310                 315                 320 


Asp Gly Gly Leu Ala Val Lys Val Gly Asp Asp Tyr Tyr Ser Ala Thr 
                325                 330                 335     


Gln Asn Lys Asp Gly Ser Ile Ser Ile Asn Thr Thr Lys Tyr Thr Ala 
            340                 345                 350         


Asp Asp Gly Thr Ser Lys Thr Ala Leu Asn Lys Leu Gly Gly Ala Asp 
        355                 360                 365             


Gly Lys Thr Glu Val Val Ser Ile Gly Gly Lys Thr Tyr Ala Ala Ser 
    370                 375                 380                 


Lys Ala Glu Gly His Asn Phe Lys Ala Gln Pro Asp Leu Ala Glu Ala 
385                 390                 395                 400 


Ala Ala Thr Thr Thr Glu Asn Pro Leu Gln Lys Ile Asp Ala Ala Leu 
                405                 410                 415     


Ala Gln Val Asp Thr Leu Arg Ser Asp Leu Gly Ala Val Gln Asn Arg 
            420                 425                 430         


Phe Asn Ser Ala Ile Thr Asn Leu Gly Asn Thr Val Asn Asn Leu Thr 
        435                 440                 445             


Ser Ala Arg Ser Arg Ile Glu Asp Ser Asp Tyr Ala Thr Glu Val Ser 
    450                 455                 460                 


Asn Met Ser Arg Ala Gln Ile Leu Gln Gln Ala Gly Thr Ser Val Leu 
465                 470                 475                 480 


Ala Gln Ala Asn Gln Val Pro Gln Asn Val Leu Ser Leu Leu Arg 
                485                 490                 495 


<210>  4
<211>  1485
<212>  DNA
<213>  Salmonella typhimurium

<400>  4
atggcacaag tcattaatac aaacagcctg tcgctgttga cccagaataa cctgaacaaa       60

tcccagtccg ctctgggcac cgctatcgag cgtctgtctt ccggtctgcg tatcaacagc      120

gcgaaagacg atgcggcagg tcaggcgatt gctaaccgtt ttaccgcgaa catcaaaggt      180

ctgactcagg cttcccgtaa cgctaacgac ggtatctcca ttgcgcagac cactgaaggc      240

gcgctgaacg aaatcaacaa caacctgcag cgtgtgcgtg aactggcggt tcagtctgct      300

aacagcacca actcccagtc tgacctcgac tccatccagg ctgaaatcac ccagcgcctg      360

aacgaaatcg accgtgtatc cggccagact cagttcaacg gcgtgaaagt cctggcgcag      420

gacaacaccc tgaccatcca ggttggtgcc aacgacggtg aaactatcga tatcgatctg      480

aagcagatca actctcagac cctgggtctg gatacgctga atgtgcaaca aaaatataag      540

gtcagcgata cggctgcaac tgttacagga tatgccgata ctacgattgc tttagacaat      600

agtactttta aagcctcggc tactggtctt ggtggtactg accagaaaat tgatggcgat      660

ttaaaatttg atgatacgac tggaaaatat tacgccaaag ttaccgttac ggggggaact      720

ggtaaagatg gctattatga agtttccgtt gataagacga acggtgaggt gactcttgct      780

ggcggtgcga cttccccgct tacaggtgga ctacctgcga cagcaactga ggatgtgaaa      840

aatgtacaag ttgcaaatgc tgatttgaca gaggctaaag ccgcattgac agcagcaggt      900

gttaccggca cagcatctgt tgttaagatg tcttatactg ataataacgg taaaactatt      960

gatggtggtt tagcagttaa ggtaggcgat gattactatt ctgcaactca aaataaagat     1020

ggttccataa gtattaatac tacgaaatac actgcagatg acggtacatc caaaactgca     1080

ctaaacaaac tgggtggcgc agacggcaaa accgaagttg tttctattgg tggtaaaact     1140

tacgctgcaa gtaaagccga aggtcacaac tttaaagcac agcctgatct ggcggaagcg     1200

gctgctacaa ccaccgaaaa cccgctgcag aaaattgatg ctgctttggc acaggttgac     1260

acgttacgtt ctgacctggg tgcggtacag aaccgtttca actccgctat taccaacctg     1320

ggcaacaccg taaacaacct gacttctgcc cgtagccgta tcgaagattc cgactacgcg     1380

accgaagttt ccaacatgtc tcgcgcgcag attctgcagc aggccggtac ctccgttctg     1440

gcgcaggcga accaggttcc gcaaaacgtc ctctctttac tgcgt                     1485


<210>  5
<211>  1018
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fg115

<400>  5

Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn 
1               5                   10                  15      


Asn Leu Asn Lys Ser Gln Ser Ala Leu Gly Thr Ala Ile Glu Arg Leu 
            20                  25                  30          


Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln 
        35                  40                  45              


Ala Ile Ala Asn Arg Phe Thr Ala Asn Ile Lys Gly Leu Thr Gln Ala 
    50                  55                  60                  


Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly 
65                  70                  75                  80  


Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ala 
                85                  90                  95      


Val Gln Ser Ala Asn Ser Thr Asn Ser Gln Ser Asp Leu Asp Ser Ile 
            100                 105                 110         


Gln Ala Glu Ile Thr Gln Arg Leu Asn Glu Ile Asp Arg Val Ser Gly 
        115                 120                 125             


Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ala Gln Asp Asn Thr Leu 
    130                 135                 140                 


Thr Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Asp Ile Asp Leu 
145                 150                 155                 160 


Lys Gln Ile Asn Ser Gln Thr Leu Gly Leu Asp Thr Leu Asn Val Gln 
                165                 170                 175     


Gln Lys Tyr Lys Val Ser Asp Thr Ala Ala Thr Val Thr Gly Tyr Ala 
            180                 185                 190         


Asp Thr Thr Ile Ala Leu Asp Asn Ser Thr Phe Lys Ala Ser Ala Thr 
        195                 200                 205             


Gly Leu Gly Gly Thr Asp Gln Lys Ile Asp Gly Asp Leu Lys Phe Asp 
    210                 215                 220                 


Asp Thr Thr Gly Lys Tyr Tyr Ala Lys Val Thr Val Thr Gly Gly Thr 
225                 230                 235                 240 


Gly Lys Asp Gly Tyr Tyr Glu Val Ser Val Asp Lys Thr Asn Gly Glu 
                245                 250                 255     


Val Thr Leu Ala Gly Gly Ala Thr Ser Pro Leu Thr Gly Gly Leu Pro 
            260                 265                 270         


Ala Thr Ala Thr Glu Asp Val Lys Asn Val Gln Val Ala Asn Ala Asp 
        275                 280                 285             


Leu Thr Glu Ala Lys Ala Ala Leu Thr Ala Ala Gly Val Thr Gly Thr 
    290                 295                 300                 


Ala Ser Val Val Lys Met Ser Tyr Thr Asp Asn Asn Gly Lys Thr Ile 
305                 310                 315                 320 


Asp Gly Gly Leu Ala Val Lys Val Gly Asp Asp Tyr Tyr Ser Ala Thr 
                325                 330                 335     


Gln Asn Lys Asp Gly Ser Ile Ser Ile Asn Thr Thr Lys Tyr Thr Ala 
            340                 345                 350         


Asp Asp Gly Thr Ser Lys Thr Ala Leu Asn Lys Leu Gly Gly Ala Asp 
        355                 360                 365             


Gly Lys Thr Glu Val Val Ser Ile Gly Gly Lys Thr Tyr Ala Ala Ser 
    370                 375                 380                 


Lys Ala Glu Gly His Asn Phe Lys Ala Gln Pro Asp Leu Ala Glu Ala 
385                 390                 395                 400 


Ala Ala Thr Thr Thr Glu Asn Pro Leu Gln Lys Ile Asp Ala Ala Leu 
                405                 410                 415     


Ala Gln Val Asp Thr Leu Arg Ser Asp Leu Gly Ala Val Gln Asn Arg 
            420                 425                 430         


Phe Asn Ser Ala Ile Thr Asn Leu Gly Asn Thr Val Asn Asn Leu Thr 
        435                 440                 445             


Ser Ala Arg Ser Arg Ile Glu Asp Ser Asp Tyr Ala Thr Glu Val Ser 
    450                 455                 460                 


Asn Met Ser Arg Ala Gln Ile Leu Gln Gln Ala Gly Thr Ser Val Leu 
465                 470                 475                 480 


Ala Gln Ala Asn Gln Val Pro Gln Asn Val Leu Ser Leu Leu Arg Thr 
                485                 490                 495     


Ser Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp 
            500                 505                 510         


Lys Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr 
        515                 520                 525             


Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu 
    530                 535                 540                 


Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr 
545                 550                 555                 560 


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
                565                 570                 575     


Asn Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met 
            580                 585                 590         


Trp Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu 
        595                 600                 605             


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
    610                 615                 620                 


Thr Leu Asn Cys Thr Asp Leu Arg Asn Val Thr Asn Ile Asn Asn Ser 
625                 630                 635                 640 


Ser Glu Gly Met Arg Gly Glu Ile Lys Asn Cys Ser Phe Asn Ile Thr 
                645                 650                 655     


Thr Ser Ile Arg Asp Lys Val Lys Lys Asp Tyr Ala Leu Phe Tyr Arg 
            660                 665                 670         


Leu Asp Val Val Pro Ile Asp Asn Asp Asn Thr Ser Tyr Arg Leu Ile 
        675                 680                 685             


Asn Cys Asn Thr Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Ser Phe 
    690                 695                 700                 


Glu Pro Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Phe Ala Ile Leu 
705                 710                 715                 720 


Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys Lys Asn Val 
                725                 730                 735     


Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln 
            740                 745                 750         


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val Ile Arg Ser 
        755                 760                 765             


Ser Asn Phe Thr Asp Asn Ala Lys Asn Ile Ile Val Gln Leu Lys Glu 
    770                 775                 780                 


Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 
785                 790                 795                 800 


Ile His Ile Gly Pro Gly Arg Ala Phe Tyr Thr Thr Gly Asp Ile Ile 
                805                 810                 815     


Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Thr Lys Trp Asn 
            820                 825                 830         


Asn Thr Leu Asn Gln Ile Ala Thr Lys Leu Lys Glu Gln Phe Gly Asn 
        835                 840                 845             


Asn Lys Thr Ile Val Phe Asn Gln Ser Ser Gly Gly Asp Pro Glu Ile 
    850                 855                 860                 


Val Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser 
865                 870                 875                 880 


Thr Gln Leu Phe Asn Ser Thr Trp Asn Phe Asn Gly Thr Trp Asn Leu 
                885                 890                 895     


Thr Gln Ser Asn Gly Thr Glu Gly Asn Asp Thr Ile Thr Leu Pro Cys 
            900                 905                 910         


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met 
        915                 920                 925             


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
    930                 935                 940                 


Gly Leu Ile Leu Thr Arg Asp Gly Gly Asn Asn His Asn Asn Asp Thr 
945                 950                 955                 960 


Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser 
                965                 970                 975     


Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala 
            980                 985                 990         


Pro Thr Lys Ala Lys Arg Arg Val  Val Gln Arg Glu Lys  Arg Leu Val 
        995                 1000                 1005             


Pro Arg  Gly Ser His His His  His His His 
    1010                 1015             


<210>  6
<211>  3075
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Fg115

<400>  6
ggatccatgg cacaagtcat taatacaaac agcctgtcgc tgttgaccca gaataacctg       60

aacaaatccc agtccgctct gggcaccgct atcgagcgtc tgtcttccgg tctgcgtatc      120

aacagcgcga aagacgatgc ggcaggtcag gcgattgcta accgttttac cgcgaacatc      180

aaaggtctga ctcaggcttc ccgtaacgct aacgacggta tctccattgc gcagaccact      240

gaaggcgcgc tgaacgaaat caacaacaac ctgcagcgtg tgcgtgaact ggcggttcag      300

tctgctaaca gcaccaactc ccagtctgac ctcgactcca tccaggctga aatcacccag      360

cgcctgaacg aaatcgaccg tgtatccggc cagactcagt tcaacggcgt gaaagtcctg      420

gcgcaggaca acaccctgac catccaggtt ggtgccaacg acggtgaaac tatcgatatc      480

gatctgaagc agatcaactc tcagaccctg ggtctggata cgctgaatgt gcaacaaaaa      540

tataaggtca gcgatacggc tgcaactgtt acaggatatg ccgatactac gattgcttta      600

gacaatagta cttttaaagc ctcggctact ggtcttggtg gtactgacca gaaaattgat      660

ggcgatttaa aatttgatga tacgactgga aaatattacg ccaaagttac cgttacgggg      720

ggaactggta aagatggcta ttatgaagtt tccgttgata agacgaacgg tgaggtgact      780

cttgctggcg gtgcgacttc cccgcttaca ggtggactac ctgcgacagc aactgaggat      840

gtgaaaaatg tacaagttgc aaatgctgat ttgacagagg ctaaagccgc attgacagca      900

gcaggtgtta ccggcacagc atctgttgtt aagatgtctt atactgataa taacggtaaa      960

actattgatg gtggtttagc agttaaggta ggcgatgatt actattctgc aactcaaaat     1020

aaagatggtt ccataagtat taatactacg aaatacactg cagatgacgg tacatccaaa     1080

actgcactaa acaaactggg tggcgcagac ggcaaaaccg aagttgtttc tattggtggt     1140

aaaacttacg ctgcaagtaa agccgaaggt cacaacttta aagcacagcc tgatctggcg     1200

gaagcggctg ctacaaccac cgaaaacccg ctgcagaaaa ttgatgctgc tttggcacag     1260

gttgacacgt tacgttctga cctgggtgcg gtacagaacc gtttcaactc cgctattacc     1320

aacctgggca acaccgtaaa caacctgact tctgcccgta gccgtatcga agattccgac     1380

tacgcgaccg aagtttccaa catgtctcgc gcgcagattc tgcagcaggc cggtacctcc     1440

gttctggcgc aggcgaacca ggttccgcaa aacgtcctct ctttactgcg tactagtatg     1500

agagtgaagg agaagtatca gcacttgtgg agatgggggt ggaaatgggg caccatgctc     1560

cttgggatat tgatgatctg tagtgctaca gaaaaattgt gggtcacagt ctattatggg     1620

gtacctgtgt ggaaagaagc aaccaccact ctattttgtg catcagatgc taaagcatat     1680

gatacagagg tacataatgt ttgggccaca catgcctgtg tacccacaga ccccaaccca     1740

caagaagtag tattggaaaa tgtgacagaa aattttaaca tgtggaaaaa taacatggta     1800

gaacagatgc atgaggatat aatcagttta tgggatcaaa gcctaaagcc atgtgtaaaa     1860

ttaaccccac tctgtgttac tttaaattgc actgatttga ggaatgttac taatatcaat     1920

aatagtagtg agggaatgag aggagaaata aaaaactgct ctttcaatat caccacaagc     1980

ataagagata aggtgaagaa agactatgca cttttttata gacttgatgt agtaccaata     2040

gataatgata atactagcta taggttgata aattgtaata cctcaaccat tacacaggcc     2100

tgtccaaagg tatcctttga gccaattccc atacattatt gtaccccggc tggttttgcg     2160

attctaaagt gtaaagataa gaagttcaat ggaacagggc catgtaaaaa tgtcagcaca     2220

gtacaatgta cacatggaat taggccagta gtgtcaactc aactgctgtt aaatggcagt     2280

ctagcagaag aagaggtagt aattagatct agtaatttca cagacaatgc aaaaaacata     2340

atagtacagt tgaaagaatc tgtagaaatt aattgtacaa gacccaacaa caatacaagg     2400

aaaagtatac atataggacc aggaagagca ttttatacaa caggagacat aataggagat     2460

ataagacaag cacattgcaa cattagtaga acaaaatgga ataacacttt aaatcaaata     2520

gctacaaaat taaaagaaca atttgggaat aataaaacaa tagtctttaa tcaatcctca     2580

ggaggggacc cagaaattgt aatgcacagt tttaattgtg gaggggaatt tttctactgt     2640

aattcaacac aactgtttaa tagtacttgg aattttaatg gtacttggaa tttaacacaa     2700

tcgaatggta ctgaaggaaa tgacactatc acactcccat gtagaataaa acaaattata     2760

aacatgtggc aagaagtagg aaaagcaatg tatgcccctc ccatcagagg acaaattaga     2820

tgttcatcaa atattacagg gctgatatta acaagagatg gtggaaataa ccacaataat     2880

gataccgaga cctttagacc tggaggagga gatatgaggg acaattggag aagtgaatta     2940

tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga     3000

agagtggtgc agagagaaaa aagactggtt ccgcgtggat ctcaccatca ccatcaccat     3060

taagtaagcg gccgc                                                      3075


<210>  7
<211>  6
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Linker

<400>  7
actagt                                                                   6


<210>  8
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Thrombin cleavage sequence

<400>  8

Leu Val Pro Arg Gly Ser 
1               5       


<210>  9
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Thrombin cleavage sequence

<400>  9
ctggttccgc gtggatct                                                     18


<210>  10
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His(6) tag

<400>  10

His His His His His His 
1               5       


<210>  11
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  His(6) tag

<400>  11
caccatcacc atcaccat                                                     18


<210>  12
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FlgT peptide

<400>  12

His His His His His His His His His His His His Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ala Val Gln Ser 
            20                  25                  30          


Ala Asn Ser Thr Asn Ser Gln Ser Asp Leu Asp Ser 
        35                  40                  




Document11-9/10/2015




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