                         SEQUENCE LISTING

<110>  Absynth Biologics Limited
 
<120>  Div1B Peptide Vaccine

<130>  4278P/WO

<150>  GB1417214.2
<151>  2014-09-30

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  440
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Met Asp Asp Lys Thr Lys Asn Asp Gln Gln Glu Ser Asn Glu Asp 
1               5                   10                  15      


Lys Asp Glu Leu Glu Leu Phe Thr Arg Asn Thr Ser Lys Lys Arg Arg 
            20                  25                  30          


Gln Arg Lys Arg Ser Lys Ala Thr His Phe Ser Asn Gln Asn Lys Asp 
        35                  40                  45              


Asp Thr Ser Gln Gln Ala Asp Phe Asp Glu Glu Ile Tyr Leu Ile Asn 
    50                  55                  60                  


Lys Asp Phe Lys Lys Glu Glu Ser Asn Asp Lys Asn Asn Asp Ser Ala 
65                  70                  75                  80  


Ser Ser His Ala Asn Asp Asn Asn Ile Asp Asp Ser Thr Asp Ser Asn 
                85                  90                  95      


Ile Glu Asn Glu Asp Tyr Arg Tyr Asn Gln Glu Ile Asp Asp Gln Asn 
            100                 105                 110         


Glu Ser Asn Val Ile Ser Val Asp Asn Glu Gln Pro Gln Ser Ala Pro 
        115                 120                 125             


Lys Glu Gln Asn Ser Asp Ser Ile Asp Glu Glu Thr Val Thr Lys Lys 
    130                 135                 140                 


Glu Arg Lys Ser Lys Val Thr Gln Leu Lys Pro Leu Thr Leu Glu Glu 
145                 150                 155                 160 


Lys Arg Lys Leu Arg Arg Lys Arg Gln Lys Arg Ile Gln Tyr Ser Val 
                165                 170                 175     


Ile Thr Ile Leu Val Leu Leu Ile Ala Val Ile Leu Ile Tyr Met Phe 
            180                 185                 190         


Ser Pro Leu Ser Lys Ile Ala His Val Asn Ile Asn Gly Asn Asn His 
        195                 200                 205             


Val Ser Thr Ser Lys Ile Asn Lys Val Leu Gly Val Lys Asn Asp Ser 
    210                 215                 220                 


Arg Met Tyr Thr Phe Ser Lys Lys Asn Ala Ile Asn Asp Leu Glu Glu 
225                 230                 235                 240 


Asn Pro Leu Ile Lys Ser Val Glu Ile His Lys Gln Leu Pro Asn Thr 
                245                 250                 255     


Leu Asn Val Asp Ile Thr Glu Asn Glu Ile Ile Ala Leu Val Lys Tyr 
            260                 265                 270         


Lys Gly Lys Tyr Leu Pro Leu Leu Glu Asn Gly Lys Leu Leu Lys Gly 
        275                 280                 285             


Ser Asn Asp Val Lys Ile Asn Asp Ala Pro Val Met Asp Gly Phe Lys 
    290                 295                 300                 


Gly Thr Lys Glu Asp Asp Met Ile Lys Ala Leu Ser Glu Met Thr Pro 
305                 310                 315                 320 


Glu Val Arg Arg Tyr Ile Ala Glu Val Thr Tyr Ala Pro Ser Lys Asn 
                325                 330                 335     


Lys Gln Ser Arg Ile Glu Leu Phe Thr Thr Asp Gly Leu Gln Val Ile 
            340                 345                 350         


Gly Asp Ile Ser Thr Ile Ser Lys Lys Met Lys Tyr Tyr Pro Gln Met 
        355                 360                 365             


Ser Gln Ser Leu Ser Arg Asp Ser Ser Gly Lys Leu Lys Thr Arg Gly 
    370                 375                 380                 


Tyr Ile Asp Leu Ser Val Gly Ala Ser Phe Ile Pro Tyr Arg Gly Asn 
385                 390                 395                 400 


Thr Ser Ser Gln Ser Glu Ser Asp Lys Asn Val Thr Lys Ser Ser Gln 
                405                 410                 415     


Glu Glu Asn Gln Ala Lys Glu Glu Leu Gln Ser Val Leu Asn Lys Ile 
            420                 425                 430         


Asn Lys Gln Ser Ser Lys Asn Asn 
        435                 440 


<210>  2
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ECD Staphylococcus aureus

<400>  2

Pro Leu Ser Lys Ile Ala His Val Asn Ile Asn Gly Asn Asn His Val 
1               5                   10                  15      


Ser Thr Ser Lys Ile Asn Lys Val Leu Gly Val Lys Asn Asp Ser Arg 
            20                  25                  30          


Met Tyr Thr Phe Ser Lys Lys Asn Ala Ile Asn Asp Leu Glu Glu Asp 
        35                  40                  45              


Pro Leu Ile Lys Ser Val Glu Ile His Lys Gln Leu Pro Asn Thr Leu 
    50                  55                  60                  


Asn Val Asp Ile Thr Glu Asn Glu Ile Ile Ala Leu Val Lys Tyr Lys 
65                  70                  75                  80  


Gly Lys Tyr Leu Pro Leu Leu Glu Asn Gly Lys Leu Leu Lys Gly Ser 
                85                  90                  95      


Asn Asp Val Lys Ile Asn Asp Ala Pro Val Met Asp Gly Phe Lys Gly 
            100                 105                 110         


Thr Lys Glu Asp Asp Met Ile Lys Ala Leu Ser Glu Met Thr Pro Glu 
        115                 120                 125             


Val Arg Arg Tyr Ile Ala Glu Val Thr Tyr Ala Pro Ser Lys Asn Lys 
    130                 135                 140                 


Gln Ser Arg Ile Glu Leu Phe Thr Thr Asp Gly Leu Gln Val Ile Gly 
145                 150                 155                 160 


Asp Ile Ser Thr Ile Ser Lys Lys Met Lys Tyr Tyr Pro Gln Met Ser 
                165                 170                 175     


Gln Ser Leu Ser Arg Asp Ser Ser Gly Lys Leu Lys Thr Arg Gly Tyr 
            180                 185                 190         


Ile Asp Leu Ser Val Gly Ala Ser Phe Ile Pro Tyr Arg Gly Asn Thr 
        195                 200                 205             


Ser Ser Gln Ser Glu Ser Asp Lys Asn Val Thr Lys Ser Ser Gln Glu 
    210                 215                 220                 


Glu Asn Gln Ala Lys Glu Glu Leu Gln Ser Val Leu Asn Lys Ile Asn 
225                 230                 235                 240 


Lys Gln Ser Ser Lys Asn Asn 
                245         


<210>  3
<211>  82
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fragment A Staphylococcus aureus

<400>  3

Glu Asn Glu Ile Ile Ala Leu Val Lys Tyr Lys Gly Lys Tyr Leu Pro 
1               5                   10                  15      


Leu Leu Glu Asn Gly Lys Leu Leu Lys Gly Ser Asn Asp Val Lys Ile 
            20                  25                  30          


Asn Asp Ala Pro Val Met Asp Gly Phe Lys Gly Thr Lys Glu Asp Asp 
        35                  40                  45              


Met Ile Lys Ala Leu Ser Glu Met Thr Pro Glu Val Arg Arg Tyr Ile 
    50                  55                  60                  


Ala Glu Val Thr Tyr Ala Pro Ser Lys Asn Lys Gln Ser Arg Ile Glu 
65                  70                  75                  80  


Leu Phe 
        


<210>  4
<211>  124
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fragment B Staphylococcus aureus

<400>  4

Ser Glu Met Thr Pro Glu Val Arg Arg Tyr Ile Ala Glu Val Thr Tyr 
1               5                   10                  15      


Ala Pro Ser Lys Asn Lys Gln Ser Arg Ile Glu Leu Phe Thr Thr Asp 
            20                  25                  30          


Gly Leu Gln Val Ile Gly Asp Ile Ser Thr Ile Ser Lys Lys Met Lys 
        35                  40                  45              


Tyr Tyr Pro Gln Met Ser Gln Ser Leu Ser Arg Asp Ser Ser Gly Lys 
    50                  55                  60                  


Leu Lys Thr Arg Gly Tyr Ile Asp Leu Ser Val Gly Ala Ser Phe Ile 
65                  70                  75                  80  


Pro Tyr Arg Gly Asn Thr Ser Ser Gln Ser Glu Ser Asp Lys Asn Val 
                85                  90                  95      


Thr Lys Ser Ser Gln Glu Glu Asn Gln Ala Lys Glu Glu Leu Gln Ser 
            100                 105                 110         


Val Leu Asn Lys Ile Asn Lys Gln Ser Ser Lys Asn 
        115                 120                 


<210>  5
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Fragment C Staphylococcus aureus

<400>  5

Ser Lys Asn Lys Gln Ser Arg Ile Glu Leu Phe 
1               5                   10      


<210>  6
<211>  382
<212>  PRT
<213>  Streptococcus pyogenes

<400>  6

Met Ala Lys Asp Lys Glu Lys Gln Ser Asp Asp Lys Leu Val Leu Thr 
1               5                   10                  15      


Glu Trp Gln Lys Arg Asn Ile Glu Phe Leu Lys Lys Lys Lys Gln Gln 
            20                  25                  30          


Ala Glu Glu Glu Lys Lys Leu Lys Glu Lys Leu Leu Ser Asp Lys Lys 
        35                  40                  45              


Ala Gln Gln Gln Ala Gln Asn Ala Ser Glu Ala Val Glu Leu Lys Thr 
    50                  55                  60                  


Asp Glu Lys Thr Asp Ser Gln Glu Ile Glu Ser Glu Thr Thr Ser Lys 
65                  70                  75                  80  


Pro Lys Lys Thr Lys Lys Val Arg Gln Pro Lys Glu Lys Ser Ala Thr 
                85                  90                  95      


Gln Ile Ala Phe Gln Lys Ser Leu Pro Val Leu Leu Gly Ala Leu Leu 
            100                 105                 110         


Leu Met Ala Val Ser Ile Phe Met Ile Thr Pro Tyr Ser Lys Lys Lys 
        115                 120                 125             


Glu Phe Ser Val Arg Gly Asn His Gln Thr Asn Leu Asp Glu Leu Ile 
    130                 135                 140                 


Lys Ala Ser Lys Val Lys Ala Ser Asp Tyr Trp Leu Thr Leu Leu Ile 
145                 150                 155                 160 


Ser Pro Gly Gln Tyr Glu Arg Pro Ile Leu Arg Thr Ile Pro Trp Val 
                165                 170                 175     


Lys Ser Val His Leu Ser Tyr Gln Phe Pro Asn His Phe Leu Phe Asn 
            180                 185                 190         


Val Ile Glu Phe Glu Ile Ile Ala Tyr Ala Gln Val Glu Asn Gly Phe 
        195                 200                 205             


Gln Pro Ile Leu Glu Asn Gly Lys Arg Val Asp Lys Val Arg Ala Ser 
    210                 215                 220                 


Glu Leu Pro Lys Ser Phe Leu Ile Leu Asn Leu Lys Asp Glu Lys Ala 
225                 230                 235                 240 


Ile Gln Gln Leu Val Lys Gln Leu Thr Thr Leu Pro Lys Lys Leu Val 
                245                 250                 255     


Lys Asn Ile Lys Ser Val Ser Leu Ala Asn Ser Lys Thr Thr Ala Asp 
            260                 265                 270         


Leu Leu Leu Ile Glu Met His Asp Gly Asn Val Val Arg Val Pro Gln 
        275                 280                 285             


Ser Gln Leu Thr Leu Lys Leu Pro Tyr Tyr Gln Lys Leu Lys Lys Asn 
    290                 295                 300                 


Leu Glu Asn Asp Ser Ile Val Asp Met Glu Val Gly Ile Tyr Thr Thr 
305                 310                 315                 320 


Thr Gln Glu Ile Glu Asn Gln Pro Glu Val Pro Leu Thr Pro Glu Gln 
                325                 330                 335     


Asn Ala Ala Asp Lys Glu Gly Asp Lys Pro Gly Glu His Gln Glu Gln 
            340                 345                 350         


Thr Asp Asn Asp Ser Glu Thr Pro Ala Asn Gln Ser Ser Pro Gln Gln 
        355                 360                 365             


Ala Pro Pro Ser Pro Glu Thr Val Leu Glu Gln Ala His Gly 
    370                 375                 380         


<210>  7
<211>  263
<212>  PRT
<213>  artificial sequence

<220>
<223>  ECD Streptococcus pyogenes

<400>  7

Met Ile Thr Pro Tyr Ser Lys Lys Lys Glu Phe Ser Val Arg Gly Asn 
1               5                   10                  15      


His Gln Thr Asn Leu Asp Glu Leu Ile Lys Ala Ser Lys Val Lys Ala 
            20                  25                  30          


Ser Asp Tyr Trp Leu Thr Leu Leu Ile Ser Pro Gly Gln Tyr Glu Arg 
        35                  40                  45              


Pro Ile Leu Arg Thr Ile Pro Trp Val Lys Ser Val His Leu Ser Tyr 
    50                  55                  60                  


Gln Phe Pro Asn His Phe Leu Phe Asn Val Ile Glu Phe Glu Ile Ile 
65                  70                  75                  80  


Ala Tyr Ala Gln Val Glu Asn Gly Phe Gln Pro Ile Leu Glu Asn Gly 
                85                  90                  95      


Lys Arg Val Asp Lys Val Arg Ala Ser Glu Leu Pro Lys Ser Phe Leu 
            100                 105                 110         


Ile Leu Asn Leu Lys Asp Glu Lys Ala Ile Gln Gln Leu Val Lys Gln 
        115                 120                 125             


Leu Thr Thr Leu Pro Lys Lys Leu Val Lys Asn Ile Lys Ser Val Ser 
    130                 135                 140                 


Leu Ala Asn Ser Lys Thr Thr Ala Asp Leu Leu Leu Ile Glu Met His 
145                 150                 155                 160 


Asp Gly Asn Val Val Arg Val Pro Gln Ser Gln Leu Thr Leu Lys Leu 
                165                 170                 175     


Pro Tyr Tyr Gln Lys Leu Lys Lys Asn Leu Glu Asn Asp Ser Ile Val 
            180                 185                 190         


Asp Met Glu Val Gly Ile Tyr Thr Thr Thr Gln Glu Ile Glu Asn Gln 
        195                 200                 205             


Pro Glu Val Pro Leu Thr Pro Glu Gln Asn Ala Ala Asp Lys Glu Gly 
    210                 215                 220                 


Asp Lys Pro Gly Glu His Gln Glu Gln Thr Asp Asn Asp Ser Glu Thr 
225                 230                 235                 240 


Pro Ala Asn Gln Ser Ser Pro Gln Gln Ala Pro Pro Ser Pro Glu Thr 
                245                 250                 255     


Val Leu Glu Gln Ala His Gly 
            260             


<210>  8
<211>  83
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment A Streptococcus pyogenes

<400>  8

Phe Glu Ile Ile Ala Tyr Ala Gln Val Glu Asn Gly Phe Gln Pro Ile 
1               5                   10                  15      


Leu Glu Asn Gly Lys Arg Val Asp Lys Val Arg Ala Ser Glu Leu Pro 
            20                  25                  30          


Lys Ser Phe Leu Ile Leu Asn Leu Lys Asp Glu Lys Ala Ile Gln Gln 
        35                  40                  45              


Leu Val Lys Gln Leu Thr Thr Leu Pro Lys Lys Leu Val Lys Asn Ile 
    50                  55                  60                  


Lys Ser Val Ser Leu Ala Asn Ser Lys Thr Thr Ala Asp Leu Leu Leu 
65                  70                  75                  80  


Ile Glu Met 
            


<210>  9
<211>  127
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment B Streptococcus pyogenes

<400>  9

Thr Leu Pro Lys Lys Leu Val Lys Asn Ile Lys Ser Val Ser Leu Ala 
1               5                   10                  15      


Asn Ser Lys Thr Thr Ala Asp Leu Leu Leu Ile Glu Met His Asp Gly 
            20                  25                  30          


Asn Val Val Arg Val Pro Gln Ser Gln Leu Thr Leu Lys Leu Pro Tyr 
        35                  40                  45              


Tyr Gln Lys Leu Lys Lys Asn Leu Glu Asn Asp Ser Ile Val Asp Met 
    50                  55                  60                  


Glu Val Gly Ile Tyr Thr Thr Thr Gln Glu Ile Glu Asn Gln Pro Glu 
65                  70                  75                  80  


Val Pro Leu Thr Pro Glu Gln Asn Ala Ala Asp Lys Glu Gly Asp Lys 
                85                  90                  95      


Pro Gly Glu His Gln Glu Gln Thr Asp Asn Asp Ser Glu Thr Pro Ala 
            100                 105                 110         


Asn Gln Ser Ser Pro Gln Gln Ala Pro Pro Ser Pro Glu Thr Val 
        115                 120                 125         


<210>  10
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment C Streptococcus pyogenes

<400>  10

Lys Thr Thr Ala Asp Leu Leu Leu Ile Glu Met 
1               5                   10      


<210>  11
<211>  246
<212>  PRT
<213>  Clostridium difficile

<400>  11

Met Lys Lys Arg Arg Lys Leu Asn Thr Asn Asn Val Met Ile Ile Leu 
1               5                   10                  15      


Val Phe Ile Leu Met Leu Ser Phe Cys Ile Cys Ile Ile Val Lys Ser 
            20                  25                  30          


Asp Leu Phe Asp Val Lys Lys Ile Asp Val Ile Gly Asn Lys Arg Val 
        35                  40                  45              


Thr Lys Ser Asn Ile Met Lys Glu Leu Asn Val Asn Leu Asn Glu Asn 
    50                  55                  60                  


Ile Phe Ala Tyr Asn Phe Lys Asp Met Lys Asn Lys Leu Ile Lys Asn 
65                  70                  75                  80  


Pro Tyr Ile Glu Asn Val Glu Ile Lys Arg Lys Leu Pro Asn Lys Ile 
                85                  90                  95      


Ile Ile Ser Leu Lys Glu Lys Glu Ile Phe Ala Val Leu Lys Asp Glu 
            100                 105                 110         


Asp Asn Tyr Cys Tyr Ile Asp Lys Lys Gly Asn Leu Leu Glu Glu Leu 
        115                 120                 125             


Arg Gly Ser Asn Glu Ser Lys Lys Asp Leu Ile Val Asp Val Asp Tyr 
    130                 135                 140                 


Ser Ile Asp Asp Asn Lys Ser Ile Lys Phe Lys Asn Tyr Lys Thr Lys 
145                 150                 155                 160 


Glu Asn Val Phe Lys Thr Leu Asn Tyr Leu Lys Glu Glu Gly Ile Tyr 
                165                 170                 175     


Arg Lys Ile Asn Tyr Val Asn Leu Lys Lys Glu Ser Asn Ile Glu Met 
            180                 185                 190         


Leu Thr Arg Ser Asn Ile Lys Ile Leu Leu Ser Asn Asp Asp Asn Leu 
        195                 200                 205             


Asp Tyr Asn Ile Ser Arg Val Ser Lys Ile Leu Ile Asp Leu Gln Asn 
    210                 215                 220                 


Lys Asn Thr Asn Gly Gly Thr Ile Asn Leu Asn Tyr Gly Lys Leu Ala 
225                 230                 235                 240 


Val Tyr Ser Pro Glu Gly 
                245     


<210>  12
<211>  218
<212>  PRT
<213>  artificial sequence

<220>
<223>  ECD Clostridium difficile

<400>  12

Ile Val Lys Ser Asp Leu Phe Asp Val Lys Lys Ile Asp Val Ile Gly 
1               5                   10                  15      


Asn Lys Arg Val Thr Lys Ser Asn Ile Met Lys Glu Leu Asn Val Asn 
            20                  25                  30          


Leu Asn Glu Asn Ile Phe Ala Tyr Asn Phe Lys Asp Met Lys Asn Lys 
        35                  40                  45              


Leu Ile Lys Asn Pro Tyr Ile Glu Asn Val Glu Ile Lys Arg Lys Leu 
    50                  55                  60                  


Pro Asn Lys Ile Ile Ile Ser Leu Lys Glu Lys Glu Ile Phe Ala Val 
65                  70                  75                  80  


Leu Lys Asp Glu Asp Asn Tyr Cys Tyr Ile Asp Lys Lys Gly Asn Leu 
                85                  90                  95      


Leu Glu Glu Leu Arg Gly Ser Asn Glu Ser Lys Lys Asp Leu Ile Val 
            100                 105                 110         


Asp Val Asp Tyr Ser Ile Asp Asp Asn Lys Ser Ile Lys Phe Lys Asn 
        115                 120                 125             


Tyr Lys Thr Lys Glu Asn Val Phe Lys Thr Leu Asn Tyr Leu Lys Glu 
    130                 135                 140                 


Glu Gly Ile Tyr Arg Lys Ile Asn Tyr Val Asn Leu Lys Lys Glu Ser 
145                 150                 155                 160 


Asn Ile Glu Met Leu Thr Arg Ser Asn Ile Lys Ile Leu Leu Ser Asn 
                165                 170                 175     


Asp Asp Asn Leu Asp Tyr Asn Ile Ser Arg Val Ser Lys Ile Leu Ile 
            180                 185                 190         


Asp Leu Gln Asn Lys Asn Thr Asn Gly Gly Thr Ile Asn Leu Asn Tyr 
        195                 200                 205             


Gly Lys Leu Ala Val Tyr Ser Pro Glu Gly 
    210                 215             


<210>  13
<211>  56
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment A Clostridium difficile

<400>  13

Lys Glu Ile Phe Ala Val Leu Lys Asp Glu Asp Asn Tyr Cys Tyr Ile 
1               5                   10                  15      


Asp Lys Lys Gly Asn Leu Leu Glu Glu Leu Arg Gly Ser Asn Glu Ser 
            20                  25                  30          


Lys Lys Asp Leu Ile Val Asp Val Asp Tyr Ser Ile Asp Asp Asn Lys 
        35                  40                  45              


Ser Ile Lys Phe Lys Asn Tyr Lys 
    50                  55      


<210>  14
<211>  112
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment B Clostridium difficile

<400>  14

Lys Lys Asp Leu Ile Val Asp Val Asp Tyr Ser Ile Asp Asp Asn Lys 
1               5                   10                  15      


Ser Ile Lys Phe Lys Asn Tyr Lys Thr Lys Glu Asn Val Phe Lys Thr 
            20                  25                  30          


Leu Asn Tyr Leu Lys Glu Glu Gly Ile Tyr Arg Lys Ile Asn Tyr Val 
        35                  40                  45              


Asn Leu Lys Lys Glu Ser Asn Ile Glu Met Leu Thr Arg Ser Asn Ile 
    50                  55                  60                  


Lys Ile Leu Leu Ser Asn Asp Asp Asn Leu Asp Tyr Asn Ile Ser Arg 
65                  70                  75                  80  


Val Ser Lys Ile Leu Ile Asp Leu Gln Asn Lys Asn Thr Asn Gly Gly 
                85                  90                  95      


Thr Ile Asn Leu Asn Tyr Gly Lys Leu Ala Val Tyr Ser Pro Glu Gly 
            100                 105                 110         


<210>  15
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  Fragment C Clostridium difficile

<400>  15

Asp Asn Lys Ser Ile Lys Phe Lys Asn Tyr Lys 
1               5                   10      


<210>  16
<211>  341
<212>  PRT
<213>  Staphylococcus aureus

<400>  16

Met Thr Lys Asp Ile Leu Ile Leu Ala Val Glu Thr Ser Cys Asp Glu 
1               5                   10                  15      


Thr Ser Val Ser Val Ile Lys Asn Gly Arg Asp Ile Leu Ser Asn Thr 
            20                  25                  30          


Val Leu Ser Gln Ile Glu Ser His Lys Arg Phe Gly Gly Val Val Pro 
        35                  40                  45              


Glu Val Ala Ser Arg His His Val Glu Gly Ile Thr Ala Thr Ile Asn 
    50                  55                  60                  


Glu Ala Leu Gly Asp Ala Asp Val Ser Ile Glu Asp Ile Asp Ala Ile 
65                  70                  75                  80  


Ala Val Thr Glu Gly Pro Gly Leu Ile Gly Ala Leu Leu Ile Gly Val 
                85                  90                  95      


Asn Ala Ala Lys Ala Leu Ala Phe Ala Tyr Asp Lys Pro Leu Ile Pro 
            100                 105                 110         


Val His His Ile Ala Gly His Ile Tyr Ala Asn His Ile Glu Glu Pro 
        115                 120                 125             


Leu Thr Phe Pro Leu Ile Ala Leu Ile Val Ser Gly Gly His Thr Glu 
    130                 135                 140                 


Leu Val Tyr Met Lys Asp His Leu Ser Phe Glu Val Ile Gly Glu Thr 
145                 150                 155                 160 


Arg Asp Asp Ala Val Gly Glu Ala Tyr Asp Lys Val Ala Arg Thr Ile 
                165                 170                 175     


Gly Leu Asn Tyr Pro Gly Gly Pro Gln Val Asp Arg Leu Ala Ala Glu 
            180                 185                 190         


Gly Glu Asp Thr Tyr Ser Phe Pro Arg Val Trp Leu Asp Lys Asp Ser 
        195                 200                 205             


Tyr Asp Phe Ser Phe Ser Gly Leu Lys Ser Ala Val Ile Asn Gln Leu 
    210                 215                 220                 


His Asn Gln Arg Gln Lys Asn Ile Pro Ile Ile Glu Ala Asn Val Ala 
225                 230                 235                 240 


Thr Ser Phe Gln Asn Ser Val Val Glu Val Leu Thr Phe Lys Ala Ile 
                245                 250                 255     


Gln Ala Cys Lys Glu Tyr Gly Val Gln Arg Leu Ile Val Ala Gly Gly 
            260                 265                 270         


Val Ala Ser Asn Lys Gly Leu Arg Gln Ser Leu Ala Asp Gln Cys Lys 
        275                 280                 285             


Val Asn Asp Ile Gln Leu Thr Ile Pro Ser Pro Lys Leu Cys Thr Asp 
    290                 295                 300                 


Asn Ala Ala Met Ile Gly Val Ala Gly His Tyr Leu Tyr Gln Gln Gly 
305                 310                 315                 320 


Arg Phe Ala Asp Leu Ala Leu Asn Gly His Ser Asn Ile Asp Leu Glu 
                325                 330                 335     


Glu Tyr Ser Ala Glu 
            340     


<210>  17
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staphylococcus aureus GCP ECD

<400>  17

Val His His Ile Ala Gly His Ile Tyr Ala Asn His Ile Glu Glu Pro 
1               5                   10                  15      


Leu Thr Phe Pro Leu Ile Ala Leu Ile Val Ser Gly Gly His Thr Glu 
            20                  25                  30          


Leu Val Tyr Met Lys Asp His Leu Ser Phe Glu Val Ile Gly Glu Thr 
        35                  40                  45              


Arg Asp Asp Ala Val Gly Glu Ala Tyr Asp Lys Val Ala Arg Thr Ile 
    50                  55                  60                  


Gly Leu Asn Tyr Pro Gly Gly Pro Gln Val Asp Arg Leu Ala Ala Glu 
65                  70                  75                  80  


Gly Glu Asp Thr Tyr Ser Phe Pro Arg Val Trp Leu Asp Lys Asp Ser 
                85                  90                  95      


Tyr Asp Phe Ser Phe Ser Gly Leu Lys Ser Ala Val Ile Asn Gln Leu 
            100                 105                 110         


His Asn Gln Arg Gln Lys Asn Ile Pro Ile Ile Glu Ala Asn Val Ala 
        115                 120                 125             


Thr Ser Phe Gln Asn Ser Val Val Glu Val Leu Thr Phe Lys Ala Ile 
    130                 135                 140                 


Gln Ala Cys Lys Glu Tyr Gly Val Gln Arg Leu Ile Val Ala Gly Gly 
145                 150                 155                 160 


Val Ala Ser Asn Lys Gly Leu Arg Gln Ser Leu Ala Asp Gln Cys Lys 
                165                 170                 175     


Val Asn Asp Ile Gln Leu Thr Ile Pro Ser Pro Lys Leu Cys Thr Asp 
            180                 185                 190         


Asn Ala Ala Met Ile Gly Val Ala Gly His Tyr Leu Tyr Gln Gln Gly 
        195                 200                 205             


Arg Phe Ala Asp Leu Ala Leu Asn Gly His Ser Asn Ile Asp Leu Glu 
    210                 215                 220                 


Glu Tyr Ser Ala Glu 
225                 


<210>  18
<211>  338
<212>  PRT
<213>  Clostridium difficile GCP

<400>  18

Met Ser Asp Ile Ile Thr Leu Ala Ile Glu Ser Ser Cys Asp Glu Thr 
1               5                   10                  15      


Ala Ala Ser Val Leu Lys Asn Gly Arg Glu Ile Leu Ser Asn Ile Ile 
            20                  25                  30          


Ser Thr Gln Ile Glu Thr His Lys Lys Phe Gly Gly Val Val Pro Glu 
        35                  40                  45              


Val Ala Ser Arg Lys His Val Glu Asn Ile Asp Ile Val Val Gln Glu 
    50                  55                  60                  


Ala Leu Asp Lys Ala Asn Ile Gly Phe Asn Asp Ile Asp His Ile Ala 
65                  70                  75                  80  


Val Thr Tyr Gly Pro Gly Leu Val Gly Ala Leu Leu Val Gly Leu Ser 
                85                  90                  95      


Tyr Ala Lys Ala Leu Ala Tyr Thr Leu Asn Ile Pro Leu Val Gly Val 
            100                 105                 110         


Asn His Ile Glu Gly His Leu Ser Ala Asn Tyr Ile Glu His Lys Asp 
        115                 120                 125             


Leu Lys Pro Pro Phe Ile Thr Leu Ile Val Ser Gly Gly His Thr His 
    130                 135                 140                 


Leu Val Glu Val Lys Asp Tyr Gly Lys Tyr Glu Ile Leu Gly Lys Thr 
145                 150                 155                 160 


Arg Asp Asp Ala Ser Gly Glu Ala Phe Asp Lys Ile Ser Arg Ala Met 
                165                 170                 175     


Asn Leu Gly Tyr Pro Gly Gly Pro Ile Ile Asp Asn Leu Ala Lys Asn 
            180                 185                 190         


Gly Asn Lys His Ala Ile Glu Phe Pro Arg Ala Tyr Leu Glu Glu Asp 
        195                 200                 205             


Ser Tyr Asp Phe Ser Phe Ser Gly Leu Lys Ser Ser Val Leu Asn Tyr 
    210                 215                 220                 


Leu Asn Gly Lys Arg Met Lys Asn Glu Glu Ile Val Val Glu Asp Val 
225                 230                 235                 240 


Ala Ala Ser Phe Gln Glu Ala Val Val Glu Val Leu Ser Thr Lys Ala 
                245                 250                 255     


Leu Lys Ala Val Lys Asp Lys Gly Tyr Asn Ile Ile Thr Leu Ser Gly 
            260                 265                 270         


Gly Val Ala Ser Asn Ser Gly Leu Arg Ala Lys Ile Thr Glu Leu Ala 
        275                 280                 285             


Lys Asp Asn Gly Ile Thr Val Lys Tyr Pro Pro Leu Ile Leu Cys Thr 
    290                 295                 300                 


Asp Asn Ala Ala Met Ile Gly Cys Ala Gly Tyr Tyr Asn Phe Ile Asn 
305                 310                 315                 320 


Gly Lys Thr His Asp Met Ser Leu Asn Ala Val Pro Asn Leu Lys Ile 
                325                 330                 335     


Asn Gln 
        


<210>  19
<211>  342
<212>  PRT
<213>  Streptococcus pyogenes

<400>  19

Met Thr Asp Arg Tyr Ile Leu Ala Val Glu Ser Ser Cys Asp Glu Thr 
1               5                   10                  15      


Ser Val Ala Ile Leu Lys Asn Glu Ser Thr Leu Leu Ser Asn Val Ile 
            20                  25                  30          


Ala Ser Gln Val Glu Ser His Lys Arg Phe Gly Gly Val Val Pro Glu 
        35                  40                  45              


Val Ala Ser Arg His His Val Glu Val Ile Thr Thr Cys Phe Glu Asp 
    50                  55                  60                  


Ala Leu Gln Glu Ala Gly Ile Ser Ala Ser Asp Leu Ser Ala Val Ala 
65                  70                  75                  80  


Val Thr Tyr Gly Pro Gly Leu Val Gly Ala Leu Leu Val Gly Leu Ala 
                85                  90                  95      


Ala Ala Lys Ala Phe Ala Trp Ala Asn His Leu Pro Leu Ile Pro Val 
            100                 105                 110         


Asn His Met Ala Gly His Leu Met Ala Ala Arg Glu Gln Glu Pro Leu 
        115                 120                 125             


Val Tyr Pro Leu Ile Ala Leu Leu Val Ser Gly Gly His Thr Glu Leu 
    130                 135                 140                 


Val Tyr Val Pro Glu Pro Gly Asp Tyr His Ile Ile Gly Glu Thr Arg 
145                 150                 155                 160 


Asp Asp Ala Val Gly Glu Ala Tyr Asp Lys Val Gly Arg Val Met Gly 
                165                 170                 175     


Leu Thr Tyr Pro Ala Gly Arg Glu Ile Asp Gln Leu Ala His Lys Gly 
            180                 185                 190         


Gln Asp Thr Tyr His Phe Pro Arg Ala Met Ile Thr Glu Asp His Leu 
        195                 200                 205             


Glu Phe Ser Phe Ser Gly Leu Lys Ser Ala Phe Ile Asn Leu His His 
    210                 215                 220                 


Asn Ala Lys Gln Lys Gly Asn Glu Leu Ile Leu Glu Asp Leu Cys Ala 
225                 230                 235                 240 


Ser Phe Gln Ala Ala Val Leu Asp Ile Leu Leu Ala Lys Thr Lys Lys 
                245                 250                 255     


Ala Leu Ser Arg Tyr Pro Ala Lys Met Leu Val Val Ala Gly Gly Val 
            260                 265                 270         


Ala Ala Asn Gln Gly Leu Arg Asp Arg Leu Ala Gln Glu Ile Thr His 
        275                 280                 285             


Ile Glu Val Val Ile Pro Lys Leu Arg Leu Cys Gly Asp Asn Ala Gly 
    290                 295                 300                 


Met Ile Ala Leu Ala Ala Ala Ile Glu Tyr Asp Lys Gln His Phe Ala 
305                 310                 315                 320 


Asn Met Ser Leu Asn Ala Lys Pro Ser Leu Ala Phe Asp Gln Phe Pro 
                325                 330                 335     


Asp Ser Phe Val Ile Asn 
            340         


<210>  20
<211>  247
<212>  PRT
<213>  Streptococcus pyogenes GCP ECD

<400>  20

Ala Ala Ala Lys Ala Phe Ala Trp Ala Asn His Leu Pro Leu Ile Pro 
1               5                   10                  15      


Val Asn His Met Ala Gly His Leu Met Ala Ala Arg Glu Gln Glu Pro 
            20                  25                  30          


Leu Val Tyr Pro Leu Ile Ala Leu Leu Val Ser Gly Gly His Thr Glu 
        35                  40                  45              


Leu Val Tyr Val Pro Glu Pro Gly Asp Tyr His Ile Ile Gly Glu Thr 
    50                  55                  60                  


Arg Asp Asp Ala Val Gly Glu Ala Tyr Asp Lys Val Gly Arg Val Met 
65                  70                  75                  80  


Gly Leu Thr Tyr Pro Ala Gly Arg Glu Ile Asp Gln Leu Ala His Lys 
                85                  90                  95      


Gly Gln Asp Thr Tyr His Phe Pro Arg Ala Met Ile Thr Glu Asp His 
            100                 105                 110         


Leu Glu Phe Ser Phe Ser Gly Leu Lys Ser Ala Phe Ile Asn Leu His 
        115                 120                 125             


His Asn Ala Lys Gln Lys Gly Asn Glu Leu Ile Leu Glu Asp Leu Cys 
    130                 135                 140                 


Ala Ser Phe Gln Ala Ala Val Leu Asp Ile Leu Leu Ala Lys Thr Lys 
145                 150                 155                 160 


Lys Ala Leu Ser Arg Tyr Pro Ala Lys Met Leu Val Val Ala Gly Gly 
                165                 170                 175     


Val Ala Ala Asn Gln Gly Leu Arg Asp Arg Leu Ala Gln Glu Ile Thr 
            180                 185                 190         


His Ile Glu Val Val Ile Pro Lys Leu Arg Leu Cys Gly Asp Asn Ala 
        195                 200                 205             


Gly Met Ile Ala Leu Ala Ala Ala Ile Glu Tyr Asp Lys Gln His Phe 
    210                 215                 220                 


Ala Asn Met Ser Leu Asn Ala Lys Pro Ser Leu Ala Phe Asp Gln Phe 
225                 230                 235                 240 


Pro Asp Ser Phe Val Ile Asn 
                245         


<210>  21
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  21
ataataccat ggctccactt agtaaaattg cgcatg                                 36


<210>  22
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  22
ataatactcg agattattct tacttgattg tttg                                   34


<210>  23
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  23
ttttttgaat tcatcttatt aaagggtgtg agtattg                                37


<210>  24
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  24
ttttttcggc cgatttttta attattctta cttgattgt                              39


<210>  25
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  25
ttttttcggc cgtatctacg tttaatgtgt ttggta                                 36


<210>  26
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  26
ataataccat ggctaatgaa attattgctt tagtgaaata taaa                        44


<210>  27
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  27
ataataccat ggctagggat agttcgggta aactaaaa                               38


<210>  28
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  28
ataatactcg agtgataatg attgtgacat ctgcgg                                 36


<210>  29
<211>  41
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  29
ataataccat ggctagtaaa gtatcaacaa tctctgttac a                           41


<210>  30
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  30
ataatactcg agattttcat cttccttttt agcag                                  35


<210>  31
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  31
ttttttggat cccagagttg tcgtcattcc agttac                                 36


<210>  32
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  32
ttttttggta ccaattcatc tttatcttca tttgattctt                             40


<210>  33
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  33
ttttttggta ccagaattac aaagcgtttt aaaca                                  35


<210>  34
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  34
ttttttggcg tgtgaattct aacttttt                                          28


<210>  35
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  35
ataataccat ggctaaaaac aaacaaagca gaattg                                 36


<210>  36
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  36
ataataccat ggctgaaatg acacctgaag ttagacg                                37


<210>  37
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  37
ataataccat ggctaaaatt aatgatgcac ctgtc                                  35


<210>  38
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  38
ataataccat ggctgaaaat ggtaaattgc ttaaagg                                37


<210>  39
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  39
aaaaaactcg agcgtaaaca attcaattct gctt                                   34


<210>  40
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  40
ttttttggat cccacagtag ttcaccacct ttt                                    33


<210>  41
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  41
ttttttcccg ggatcgttaa gggatcaact tt                                     32


<210>  42
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
ttttttaagc tttaaaagaa agaaggaatg gtgagcaagg gcg                         43


<210>  43
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
ttttttctcg agcttgtaca gctcgtccat gc                                     32


<210>  44
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
tttttactcg agatggatga taaaacgaag aacg                                   34


<210>  45
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
ttttttggat ccttaattat tcttacttga ttgtttgtta a                           41


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
ttttttgaat tcgcgtttcg gtgatgaaga                                        30


