                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Enzymatische Cyclisierung von Geranyllinalool

<130>  PF 75027, M/54304

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2178
<212>  DNA
<213>  Zymomonas mobilis


<220>
<221>  CDS
<222>  (1)..(2178)

<400>  1
atg ggt att gac aga atg aat agc tta agt cgc ttg tta atg aag aag         48
Met Gly Ile Asp Arg Met Asn Ser Leu Ser Arg Leu Leu Met Lys Lys           
1               5                   10                  15                

att ttc ggg gct gaa aaa acc tcg tat aaa ccg gct tcc gat acc ata         96
Ile Phe Gly Ala Glu Lys Thr Ser Tyr Lys Pro Ala Ser Asp Thr Ile           
            20                  25                  30                    

atc gga acg gat acc ctg aaa aga ccg aac cgg cgg cct gaa ccg acg        144
Ile Gly Thr Asp Thr Leu Lys Arg Pro Asn Arg Arg Pro Glu Pro Thr           
        35                  40                  45                        

gca aaa gtc gac aaa acg ata ttc aag act atg ggg aat agt ctg aat        192
Ala Lys Val Asp Lys Thr Ile Phe Lys Thr Met Gly Asn Ser Leu Asn           
    50                  55                  60                            

aat acc ctt gtt tca gcc tgt gac tgg ttg atc gga caa caa aag ccc        240
Asn Thr Leu Val Ser Ala Cys Asp Trp Leu Ile Gly Gln Gln Lys Pro           
65                  70                  75                  80            

gat ggt cat tgg gtc ggt gcc gtg gaa tcc aat gct tcg atg gaa gca        288
Asp Gly His Trp Val Gly Ala Val Glu Ser Asn Ala Ser Met Glu Ala           
                85                  90                  95                

gaa tgg tgt ctg gcc ttg tgg ttt ttg ggt ctg gaa gat cat ccg ctt        336
Glu Trp Cys Leu Ala Leu Trp Phe Leu Gly Leu Glu Asp His Pro Leu           
            100                 105                 110                   

cgt cca aga ttg ggc aat gct ctt ttg gaa atg cag cgg gaa gat ggc        384
Arg Pro Arg Leu Gly Asn Ala Leu Leu Glu Met Gln Arg Glu Asp Gly           
        115                 120                 125                       

tct tgg gga gtc tat ttc ggc gct gga aat ggc gat atc aat gcc acg        432
Ser Trp Gly Val Tyr Phe Gly Ala Gly Asn Gly Asp Ile Asn Ala Thr           
    130                 135                 140                           

gtt gaa gcc tat gcg gcc ttg cgg tct ttg ggg tat tct gcc gat aat        480
Val Glu Ala Tyr Ala Ala Leu Arg Ser Leu Gly Tyr Ser Ala Asp Asn           
145                 150                 155                 160           

cct gtt ttg aaa aaa gcg gca gca tgg att gct gaa aaa ggc gga tta        528
Pro Val Leu Lys Lys Ala Ala Ala Trp Ile Ala Glu Lys Gly Gly Leu           
                165                 170                 175               

aaa aat atc cgt gtc ttt acc cgt tat tgg ctg gcg ttg atc ggg gaa        576
Lys Asn Ile Arg Val Phe Thr Arg Tyr Trp Leu Ala Leu Ile Gly Glu           
            180                 185                 190                   

tgg cct tgg gaa aag acc cct aac ctt ccc cct gaa att atc tgg ttc        624
Trp Pro Trp Glu Lys Thr Pro Asn Leu Pro Pro Glu Ile Ile Trp Phe           
        195                 200                 205                       

cct gat aat ttt gtc ttt tcg att tat aat ttt gcc caa tgg gcg cgg        672
Pro Asp Asn Phe Val Phe Ser Ile Tyr Asn Phe Ala Gln Trp Ala Arg           
    210                 215                 220                           

gca acc atg gtg ccg att gct att ctg tcc gcg aga cga cca agc cgc        720
Ala Thr Met Val Pro Ile Ala Ile Leu Ser Ala Arg Arg Pro Ser Arg           
225                 230                 235                 240           

ccg ctg cgc cct caa gac cga ttg gat gaa ctg ttt cca gaa ggc cgc        768
Pro Leu Arg Pro Gln Asp Arg Leu Asp Glu Leu Phe Pro Glu Gly Arg           
                245                 250                 255               

gct cgc ttt gat tat gaa ttg ccg aaa aaa gaa ggc atc gat ctt tgg        816
Ala Arg Phe Asp Tyr Glu Leu Pro Lys Lys Glu Gly Ile Asp Leu Trp           
            260                 265                 270                   

tcg caa ttt ttc cga acc act gac cgt gga tta cat tgg gtt cag tcc        864
Ser Gln Phe Phe Arg Thr Thr Asp Arg Gly Leu His Trp Val Gln Ser           
        275                 280                 285                       

aat ctg tta aag cgc aat agc ttg cgt gaa gcc gct atc cgt cat gtt        912
Asn Leu Leu Lys Arg Asn Ser Leu Arg Glu Ala Ala Ile Arg His Val           
    290                 295                 300                           

ttg gaa tgg att atc cgg cat cag gat gcc gat ggc ggt tgg ggt gga        960
Leu Glu Trp Ile Ile Arg His Gln Asp Ala Asp Gly Gly Trp Gly Gly           
305                 310                 315                 320           

att cag cca cct tgg gtc tat ggt ttg atg gcg tta cat ggt gaa ggc       1008
Ile Gln Pro Pro Trp Val Tyr Gly Leu Met Ala Leu His Gly Glu Gly           
                325                 330                 335               

tat cag ctt tat cat ccg gtg atg gcc aag gct ttg tcg gct ttg gat       1056
Tyr Gln Leu Tyr His Pro Val Met Ala Lys Ala Leu Ser Ala Leu Asp           
            340                 345                 350                   

gat ccc ggt tgg cga cat gac aga ggc gag tct tct tgg ata cag gcc       1104
Asp Pro Gly Trp Arg His Asp Arg Gly Glu Ser Ser Trp Ile Gln Ala           
        355                 360                 365                       

acc aat agt ccg gta tgg gat aca atg ttg gcc ttg atg gcg tta aaa       1152
Thr Asn Ser Pro Val Trp Asp Thr Met Leu Ala Leu Met Ala Leu Lys           
    370                 375                 380                           

gac gcc aag gcc gag gat cgt ttt acg ccg gaa atg gat aag gcc gcc       1200
Asp Ala Lys Ala Glu Asp Arg Phe Thr Pro Glu Met Asp Lys Ala Ala           
385                 390                 395                 400           

gat tgg ctt ttg gct cga cag gtc aaa gtc aaa ggc gat tgg tca atc       1248
Asp Trp Leu Leu Ala Arg Gln Val Lys Val Lys Gly Asp Trp Ser Ile           
                405                 410                 415               

aaa ctg ccc gat gtt gaa ccc ggt gga tgg gca ttt gaa tat gcc aat       1296
Lys Leu Pro Asp Val Glu Pro Gly Gly Trp Ala Phe Glu Tyr Ala Asn           
            420                 425                 430                   

gat cgc tat ccc gat acc gat gat acc gcc gtc gct ttg atc gcc ctt       1344
Asp Arg Tyr Pro Asp Thr Asp Asp Thr Ala Val Ala Leu Ile Ala Leu           
        435                 440                 445                       

tcc tct tat cgt gat aag gag gag tgg caa aag aaa ggc gtt gag gac       1392
Ser Ser Tyr Arg Asp Lys Glu Glu Trp Gln Lys Lys Gly Val Glu Asp           
    450                 455                 460                           

gcc att acc cgt ggg gtt aat tgg ttg atc gcc atg caa agc gaa tgt       1440
Ala Ile Thr Arg Gly Val Asn Trp Leu Ile Ala Met Gln Ser Glu Cys           
465                 470                 475                 480           

ggc ggt tgg gga gcc ttt gat aag gat aat aac aga agt atc ctt tcc       1488
Gly Gly Trp Gly Ala Phe Asp Lys Asp Asn Asn Arg Ser Ile Leu Ser           
                485                 490                 495               

aaa att cct ttt tgt gat ttc gga gaa tct att gat ccg cct tca gtc       1536
Lys Ile Pro Phe Cys Asp Phe Gly Glu Ser Ile Asp Pro Pro Ser Val           
            500                 505                 510                   

gat gta acg gcg cat gtt tta gag gcc ttt ggc acc ttg gga ctg tcc       1584
Asp Val Thr Ala His Val Leu Glu Ala Phe Gly Thr Leu Gly Leu Ser           
        515                 520                 525                       

cgc gat atg ccg gtc atc caa aaa gcg atc gac tat gtc cgt tcc gaa       1632
Arg Asp Met Pro Val Ile Gln Lys Ala Ile Asp Tyr Val Arg Ser Glu           
    530                 535                 540                           

cag gaa gcc gaa ggc gcg tgg ttt ggt cgt tgg ggc gtt aat tat atc       1680
Gln Glu Ala Glu Gly Ala Trp Phe Gly Arg Trp Gly Val Asn Tyr Ile           
545                 550                 555                 560           

tat ggc acc ggt gcg gtt ctg cct gct ttg gcg gcg atc ggt gaa gat       1728
Tyr Gly Thr Gly Ala Val Leu Pro Ala Leu Ala Ala Ile Gly Glu Asp           
                565                 570                 575               

atg acc cag cct tac atc acc aag gct tgc gat tgg ctg gtc gca cat       1776
Met Thr Gln Pro Tyr Ile Thr Lys Ala Cys Asp Trp Leu Val Ala His           
            580                 585                 590                   

cag cag gaa gac ggc ggt tgg ggc gaa agc tgc tct tcc tat atg gag       1824
Gln Gln Glu Asp Gly Gly Trp Gly Glu Ser Cys Ser Ser Tyr Met Glu           
        595                 600                 605                       

att gat tcc att ggg aag ggc cca acc acg ccg tcc cag act gct tgg       1872
Ile Asp Ser Ile Gly Lys Gly Pro Thr Thr Pro Ser Gln Thr Ala Trp           
    610                 615                 620                           

gct ttg atg ggg ttg atc gcg gcc aat cgt ccc gaa gat tat gaa gcc       1920
Ala Leu Met Gly Leu Ile Ala Ala Asn Arg Pro Glu Asp Tyr Glu Ala           
625                 630                 635                 640           

att gcc aag gga tgc cat tat ctg att gat cgc caa gag cag gat ggt       1968
Ile Ala Lys Gly Cys His Tyr Leu Ile Asp Arg Gln Glu Gln Asp Gly           
                645                 650                 655               

agc tgg aaa gaa gaa gaa ttc acc ggc acc gga ttc ccc ggt tat ggc       2016
Ser Trp Lys Glu Glu Glu Phe Thr Gly Thr Gly Phe Pro Gly Tyr Gly           
            660                 665                 670                   

gtg ggt cag acg atc aag ttg gat gat ccg gct tta tcg aaa cga ttg       2064
Val Gly Gln Thr Ile Lys Leu Asp Asp Pro Ala Leu Ser Lys Arg Leu           
        675                 680                 685                       

ctt caa ggc gct gaa ctg tca cgg gcg ttt atg ctg cgt tat gat ttt       2112
Leu Gln Gly Ala Glu Leu Ser Arg Ala Phe Met Leu Arg Tyr Asp Phe           
    690                 695                 700                           

tat cgg caa ttc ttc ccg att atg gcg tta agt cgg gca gag aga ctg       2160
Tyr Arg Gln Phe Phe Pro Ile Met Ala Leu Ser Arg Ala Glu Arg Leu           
705                 710                 715                 720           

att gat ttg aat aat tga                                               2178
Ile Asp Leu Asn Asn                                                       
                725                                                       


<210>  2
<211>  725
<212>  PRT
<213>  Zymomonas mobilis

<400>  2

Met Gly Ile Asp Arg Met Asn Ser Leu Ser Arg Leu Leu Met Lys Lys 
1               5                   10                  15      


Ile Phe Gly Ala Glu Lys Thr Ser Tyr Lys Pro Ala Ser Asp Thr Ile 
            20                  25                  30          


Ile Gly Thr Asp Thr Leu Lys Arg Pro Asn Arg Arg Pro Glu Pro Thr 
        35                  40                  45              


Ala Lys Val Asp Lys Thr Ile Phe Lys Thr Met Gly Asn Ser Leu Asn 
    50                  55                  60                  


Asn Thr Leu Val Ser Ala Cys Asp Trp Leu Ile Gly Gln Gln Lys Pro 
65                  70                  75                  80  


Asp Gly His Trp Val Gly Ala Val Glu Ser Asn Ala Ser Met Glu Ala 
                85                  90                  95      


Glu Trp Cys Leu Ala Leu Trp Phe Leu Gly Leu Glu Asp His Pro Leu 
            100                 105                 110         


Arg Pro Arg Leu Gly Asn Ala Leu Leu Glu Met Gln Arg Glu Asp Gly 
        115                 120                 125             


Ser Trp Gly Val Tyr Phe Gly Ala Gly Asn Gly Asp Ile Asn Ala Thr 
    130                 135                 140                 


Val Glu Ala Tyr Ala Ala Leu Arg Ser Leu Gly Tyr Ser Ala Asp Asn 
145                 150                 155                 160 


Pro Val Leu Lys Lys Ala Ala Ala Trp Ile Ala Glu Lys Gly Gly Leu 
                165                 170                 175     


Lys Asn Ile Arg Val Phe Thr Arg Tyr Trp Leu Ala Leu Ile Gly Glu 
            180                 185                 190         


Trp Pro Trp Glu Lys Thr Pro Asn Leu Pro Pro Glu Ile Ile Trp Phe 
        195                 200                 205             


Pro Asp Asn Phe Val Phe Ser Ile Tyr Asn Phe Ala Gln Trp Ala Arg 
    210                 215                 220                 


Ala Thr Met Val Pro Ile Ala Ile Leu Ser Ala Arg Arg Pro Ser Arg 
225                 230                 235                 240 


Pro Leu Arg Pro Gln Asp Arg Leu Asp Glu Leu Phe Pro Glu Gly Arg 
                245                 250                 255     


Ala Arg Phe Asp Tyr Glu Leu Pro Lys Lys Glu Gly Ile Asp Leu Trp 
            260                 265                 270         


Ser Gln Phe Phe Arg Thr Thr Asp Arg Gly Leu His Trp Val Gln Ser 
        275                 280                 285             


Asn Leu Leu Lys Arg Asn Ser Leu Arg Glu Ala Ala Ile Arg His Val 
    290                 295                 300                 


Leu Glu Trp Ile Ile Arg His Gln Asp Ala Asp Gly Gly Trp Gly Gly 
305                 310                 315                 320 


Ile Gln Pro Pro Trp Val Tyr Gly Leu Met Ala Leu His Gly Glu Gly 
                325                 330                 335     


Tyr Gln Leu Tyr His Pro Val Met Ala Lys Ala Leu Ser Ala Leu Asp 
            340                 345                 350         


Asp Pro Gly Trp Arg His Asp Arg Gly Glu Ser Ser Trp Ile Gln Ala 
        355                 360                 365             


Thr Asn Ser Pro Val Trp Asp Thr Met Leu Ala Leu Met Ala Leu Lys 
    370                 375                 380                 


Asp Ala Lys Ala Glu Asp Arg Phe Thr Pro Glu Met Asp Lys Ala Ala 
385                 390                 395                 400 


Asp Trp Leu Leu Ala Arg Gln Val Lys Val Lys Gly Asp Trp Ser Ile 
                405                 410                 415     


Lys Leu Pro Asp Val Glu Pro Gly Gly Trp Ala Phe Glu Tyr Ala Asn 
            420                 425                 430         


Asp Arg Tyr Pro Asp Thr Asp Asp Thr Ala Val Ala Leu Ile Ala Leu 
        435                 440                 445             


Ser Ser Tyr Arg Asp Lys Glu Glu Trp Gln Lys Lys Gly Val Glu Asp 
    450                 455                 460                 


Ala Ile Thr Arg Gly Val Asn Trp Leu Ile Ala Met Gln Ser Glu Cys 
465                 470                 475                 480 


Gly Gly Trp Gly Ala Phe Asp Lys Asp Asn Asn Arg Ser Ile Leu Ser 
                485                 490                 495     


Lys Ile Pro Phe Cys Asp Phe Gly Glu Ser Ile Asp Pro Pro Ser Val 
            500                 505                 510         


Asp Val Thr Ala His Val Leu Glu Ala Phe Gly Thr Leu Gly Leu Ser 
        515                 520                 525             


Arg Asp Met Pro Val Ile Gln Lys Ala Ile Asp Tyr Val Arg Ser Glu 
    530                 535                 540                 


Gln Glu Ala Glu Gly Ala Trp Phe Gly Arg Trp Gly Val Asn Tyr Ile 
545                 550                 555                 560 


Tyr Gly Thr Gly Ala Val Leu Pro Ala Leu Ala Ala Ile Gly Glu Asp 
                565                 570                 575     


Met Thr Gln Pro Tyr Ile Thr Lys Ala Cys Asp Trp Leu Val Ala His 
            580                 585                 590         


Gln Gln Glu Asp Gly Gly Trp Gly Glu Ser Cys Ser Ser Tyr Met Glu 
        595                 600                 605             


Ile Asp Ser Ile Gly Lys Gly Pro Thr Thr Pro Ser Gln Thr Ala Trp 
    610                 615                 620                 


Ala Leu Met Gly Leu Ile Ala Ala Asn Arg Pro Glu Asp Tyr Glu Ala 
625                 630                 635                 640 


Ile Ala Lys Gly Cys His Tyr Leu Ile Asp Arg Gln Glu Gln Asp Gly 
                645                 650                 655     


Ser Trp Lys Glu Glu Glu Phe Thr Gly Thr Gly Phe Pro Gly Tyr Gly 
            660                 665                 670         


Val Gly Gln Thr Ile Lys Leu Asp Asp Pro Ala Leu Ser Lys Arg Leu 
        675                 680                 685             


Leu Gln Gly Ala Glu Leu Ser Arg Ala Phe Met Leu Arg Tyr Asp Phe 
    690                 695                 700                 


Tyr Arg Gln Phe Phe Pro Ile Met Ala Leu Ser Arg Ala Glu Arg Leu 
705                 710                 715                 720 


Ile Asp Leu Asn Asn 
                725 


