               SEQUENCE LISTING

<110> Antikor Biopharma Limited

<120> Biological materials and uses thereof

<140> PCT/GB2015/052800
<141> 2015-09-25

<150> GB1416960.1
<151> 2014-09-25

<160> 8

<170> BiSSAP 1.2

<210> 1
<211> 816
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..816
<223> /mol_type="unassigned DNA"
      /note="DNA sequence of cleaved TCT"
      /organism="Artificial Sequence"

<400> 1
agcggtagcg gaggtagcgg acaggtgcag ctggtgcagt ctggggcaga ggtgaaaaag      60

cccggggagt ctctgaagat ctcctgtaag ggttctggat acagctttac cagctactgg     120

atcgcctggg tgcgccagat gcccgggaaa ggcctggagt acatggggct catctatcct     180

ggtgactctg acaccaaata cagcccgtcc ttccaaggcc aggtcaccat ctcagtcgac     240

aagtccgtca gcactgccta cttgcaatgg agcagtctga agccctcgga cagcgccgtg     300

tatttttgtg cgagacatga cgtgggatat tgcagtagtt ccaactgcgc agcgtggcct     360

gaatacttcc agcattgggg ccagggcacc ctggtcaccg tctcctcagg tggaggcggt     420

tcaggcggag gtggctctgg cggtggcgga tcgcagtctg tgttgacgca gccgccctca     480

gtgtctgcgg ccccaggaca gaaggtcacc atctcctgct ctggaagcag ctccaacatt     540

gggaataatt atgtatcctg gtaccagcag ctcccaggaa cagcccccaa actcctcatc     600

tatggtcaca ccaatcggcc cgcaggggtc cctgaccgat tctctggctc caagtctggc     660

acctcagcct ccctggccat cagtgggttc cggtccgagg atgaggctga ttattactgt     720

gcagcatggg atgacagcct gagtggttgg gtgttcggcg gagggaccaa gctgaccgtc     780

ctaatggcta gcatgactgg tggacagcaa atgggt                               816


<210> 2
<211> 272
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino Acid sequence of cleaved TCT

<400> 2
Ser Gly Ser Gly Gly Ser Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      
Glu Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser 
            20                  25                  30          
Gly Tyr Ser Phe Thr Ser Tyr Trp Ile Ala Trp Val Arg Gln Met Pro 
        35                  40                  45              
Gly Lys Gly Leu Glu Tyr Met Gly Leu Ile Tyr Pro Gly Asp Ser Asp 
    50                  55                  60                  
Thr Lys Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Val Asp 
65                  70                  75                  80  
Lys Ser Val Ser Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Pro Ser 
                85                  90                  95      
Asp Ser Ala Val Tyr Phe Cys Ala Arg His Asp Val Gly Tyr Cys Ser 
            100                 105                 110         
Ser Ser Asn Cys Ala Ala Trp Pro Glu Tyr Phe Gln His Trp Gly Gln 
        115                 120                 125             
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 
Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser 
145                 150                 155                 160 
Val Ser Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser 
                165                 170                 175     
Ser Ser Asn Ile Gly Asn Asn Tyr Val Ser Trp Tyr Gln Gln Leu Pro 
            180                 185                 190         
Gly Thr Ala Pro Lys Leu Leu Ile Tyr Gly His Thr Asn Arg Pro Ala 
        195                 200                 205             
Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser 
    210                 215                 220                 
Leu Ala Ile Ser Gly Phe Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys 
225                 230                 235                 240 
Ala Ala Trp Asp Asp Ser Leu Ser Gly Trp Val Phe Gly Gly Gly Thr 
                245                 250                 255     
Lys Leu Thr Val Leu Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
            260                 265                 270         



<210> 3
<211> 838
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..838
<223> /mol_type="unassigned DNA"
      /note="DNA sequence of cleaved TCT1067 "
      /organism="Artificial Sequence"

<400> 3
gcggtagcgg aggtagcgga caggtgcagc tggtgcagtc tggggcagag gtgaaaaagc      60

ccggggagtc tctgaagatc tcctgtaagg gttctggata cagctttacc agctactgga     120

tcgcctgggt gcgccagatg cccgggaaag gcctggagta catggggctc atctatcctg     180

gtgactctga caccaaatac agcccgtcct tccaaggcca ggtcaccatc tcagtcgaca     240

agtccgtcag cactgcctac ttgcaatgga gcagtctgaa gccctcggac agcgccgtgt     300

atttttgtgc gagacatgac gtgggatatt gcaccgatcg tacctgcgca gcgtggcctg     360

aatggctggg cgtgtggggc cagggcaccc tggtcaccgt ctcctcaggt ggaggcggtt     420

caggcggagg tggctctggc ggtggcggat cgcagtctgt gttgacgcag ccgccctcag     480

tgtctgcggc cccaggacag aaggtcacca tctcctgctc tggaagcagc tccaacattg     540

ggaataatta tgtatcctgg taccagcagc tcccaggaac agcccccaaa ctcctcatct     600

atggtcacac caatcggccc gcaggggtcc ctgaccgatt ctctggctcc aagtctggca     660

cctcagcctc cctggccatc agtgggttcc ggtccgagga tgaggctgat tattactgtg     720

caagctggga ttataccctg agtggttggg tgttcggcgg agggaccaag ctgaccgtcc     780

taatggctag catgactggt ggacagcaaa tgggttgatg aggctctaac tctcctct       838


<210> 4
<211> 272
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino Acid sequence of cleaved scFv (TCT1067)

<400> 4
Ser Gly Ser Gly Gly Ser Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
1               5                   10                  15      
Glu Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser 
            20                  25                  30          
Gly Tyr Ser Phe Thr Ser Tyr Trp Ile Ala Trp Val Arg Gln Met Pro 
        35                  40                  45              
Gly Lys Gly Leu Glu Tyr Met Gly Leu Ile Tyr Pro Gly Asp Ser Asp 
    50                  55                  60                  
Thr Lys Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Val Asp 
65                  70                  75                  80  
Lys Ser Val Ser Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Pro Ser 
                85                  90                  95      
Asp Ser Ala Val Tyr Phe Cys Ala Arg His Asp Val Gly Tyr Cys Thr 
            100                 105                 110         
Asp Arg Thr Cys Ala Ala Trp Pro Glu Trp Leu Gly Val Trp Gly Gln 
        115                 120                 125             
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 
Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser 
145                 150                 155                 160 
Val Ser Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser 
                165                 170                 175     
Ser Ser Asn Ile Gly Asn Asn Tyr Val Ser Trp Tyr Gln Gln Leu Pro 
            180                 185                 190         
Gly Thr Ala Pro Lys Leu Leu Ile Tyr Gly His Thr Asn Arg Pro Ala 
        195                 200                 205             
Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser 
    210                 215                 220                 
Leu Ala Ile Ser Gly Phe Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys 
225                 230                 235                 240 
Ala Ser Trp Asp Tyr Thr Leu Ser Gly Trp Val Phe Gly Gly Gly Thr 
                245                 250                 255     
Lys Leu Thr Val Leu Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
            260                 265                 270         



<210> 5
<211> 257
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of panitumumab single chain Fv

<400> 5
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Val Ser Ser Gly 
            20                  25                  30          
Asp Tyr Tyr Trp Thr Trp Ile Arg Gln Ser Pro Gly Lys Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly His Ile Tyr Tyr Ser Gly Asn Thr Asn Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Lys Ser Arg Leu Thr Ile Ser Ile Asp Thr Ser Lys Thr Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      
Cys Val Arg Asp Arg Val Thr Gly Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         
Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Thr Val Ile Thr Cys Gln Ala Ser 
145                 150                 155                 160 
Gln Asp Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Ala Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Glu Thr Gly Val 
            180                 185                 190         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys Gln His 
    210                 215                 220                 
Phe Asp His Leu Pro Leu Ala Phe Gly Gly Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 
Gln Asn Gly Ser Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Ala Ala 
                245                 250                 255     
Ala 
    

<210> 6
<211> 254
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of scFv (TCT1067)

<400> 6
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          
Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Tyr Met 
        35                  40                  45              
Gly Leu Ile Tyr Pro Gly Asp Ser Asp Thr Lys Tyr Ser Pro Ser Phe 
    50                  55                  60                  
Gln Gly Gln Val Thr Ile Ser Val Asp Lys Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Trp Ser Ser Leu Lys Pro Ser Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      
Ala Arg His Asp Val Gly Tyr Cys Thr Asp Arg Thr Cys Ala Ala Trp 
            100                 105                 110         
Pro Glu Trp Leu Gly Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
145                 150                 155                 160 
Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Asn Asn 
                165                 170                 175     
Tyr Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
            180                 185                 190         
Ile Tyr Gly His Thr Asn Arg Pro Ala Gly Val Pro Asp Arg Phe Ser 
        195                 200                 205             
Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Phe Arg 
    210                 215                 220                 
Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp Tyr Thr Leu 
225                 230                 235                 240 
Ser Gly Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
                245                 250                 

<210> 7
<211> 241
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of scFv (panitumumab)

<400> 7
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Val Ser Ser Gly 
            20                  25                  30          
Asp Tyr Tyr Trp Thr Trp Ile Arg Gln Ser Pro Gly Lys Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly His Ile Tyr Tyr Ser Gly Asn Thr Asn Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Lys Ser Arg Leu Thr Ile Ser Ile Asp Thr Ser Lys Thr Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      
Cys Val Arg Asp Arg Val Thr Gly Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         
Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Thr Val Ile Thr Cys Gln Ala Ser 
145                 150                 155                 160 
Gln Asp Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Ala Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Glu Thr Gly Val 
            180                 185                 190         
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys Gln His 
    210                 215                 220                 
Phe Asp His Leu Pro Leu Ala Phe Gly Gly Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 
Gln 
    

<210> 8
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker region

<400> 8
Gly Ser Gly Gly Ser Gly 
1               5       

