﻿               SEQUENCE LISTING

<110> Nxt2B

<120> Phe-free proteins for treatment of phenylketonuria

<130> P014488PCT1

<160> 80

<170> BiSSAP 1.3

<210> 1
<211> 224
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 1
Asn Ile Lys Ile Ala Val Ile Asp Ser Gly Ile Ser Pro His Asp Asp 
1               5                   10                  15      
Leu Ser Ile Ala Gly Gly Tyr Ser Ala Val Ser Tyr Thr Ser Ser Tyr 
            20                  25                  30          
Lys Asp Asp Asn Gly His Gly Thr His Val Ala Gly Ile Ile Gly Ala 
        35                  40                  45              
Lys His Asn Gly Tyr Gly Ile Asp Gly Ile Ala Pro Glu Ala Gln Ile 
    50                  55                  60                  
Tyr Ala Val Lys Ala Leu Asp Gln Asn Gly Ser Gly Asp Leu Gln Ser 
65                  70                  75                  80  
Leu Leu Gln Gly Ile Asp Trp Ser Ile Ala Asn Arg Met Asp Ile Val 
                85                  90                  95      
Asn Met Ser Leu Gly Thr Thr Ser Asp Ser Lys Ile Leu His Asp Ala 
            100                 105                 110         
Val Asn Lys Ala Tyr Glu Gln Gly Val Leu Leu Val Ala Ala Ser Gly 
        115                 120                 125             
Asn Asp Gly Asn Gly Lys Pro Val Asn Tyr Pro Ala Ala Tyr Ser Ser 
    130                 135                 140                 
Val Val Ala Val Ser Ala Thr Asn Glu Lys Asn Gln Leu Ala Ser Phe 
145                 150                 155                 160 
Ser Thr Thr Gly Asp Glu Val Glu Phe Ser Ala Pro Gly Thr Asn Ile 
                165                 170                 175     
Thr Ser Thr Tyr Leu Asn Gln Tyr Tyr Ala Thr Gly Ser Gly Thr Ser 
            180                 185                 190         
Gln Ala Thr Pro His Ala Ala Ala Met Phe Ala Leu Leu Lys Gln Arg 
        195                 200                 205             
Asp Pro Ala Glu Thr Asn Val Gln Leu Arg Glu Glu Met Arg Lys Asn 
    210                 215                 220                 



<210> 2
<211> 183
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 2
Gly Lys Gly Ile Lys Val Ala Ile Ile Asp Thr Gly Val Glu Tyr Asn 
1               5                   10                  15      
His Pro Asp Leu Lys Lys Asn Phe Gly Gln Tyr Lys Gly Tyr Asp Phe 
            20                  25                  30          
Val Asp Asn Asp Tyr Asp Pro Lys Glu Thr Pro Thr Gly Asp Pro Arg 
        35                  40                  45              
Gly Glu Ala Thr Asp His Gly Thr His Val Ala Gly Thr Val Ala Ala 
    50                  55                  60                  
Asn Gly Thr Ile Lys Gly Val Ala Pro Asp Ala Thr Leu Leu Ala Tyr 
65                  70                  75                  80  
Arg Val Leu Gly Pro Gly Gly Ser Gly Thr Thr Glu Asn Val Ile Ala 
                85                  90                  95      
Gly Val Glu Arg Ala Val Gln Asp Gly Ala Asp Val Met Asn Leu Ser 
            100                 105                 110         
Leu Gly Asn Ser Leu Asn Asn Pro Asp Trp Ala Thr Ser Thr Ala Leu 
        115                 120                 125             
Asp Trp Ala Met Ser Glu Gly Val Val Ala Val Thr Ser Asn Gly Asn 
    130                 135                 140                 
Ser Gly Pro Asn Gly Trp Thr Val Gly Ser Pro Gly Thr Ser Arg Glu 
145                 150                 155                 160 
Ala Ile Ser Val Gly Ala Thr Gln Leu Pro Leu Asn Glu Tyr Ala Val 
                165                 170                 175     
Thr Phe Gly Ser Tyr Ser Ser 
            180             

<210> 3
<211> 181
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 3
Trp Ser Phe Asn Thr Leu Lys His Asn Met Lys Asp Ile His Asp Ala 
1               5                   10                  15      
Gly Tyr Thr Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys Glu Gly 
            20                  25                  30          
Asn Gln Gly Asn Lys Ser Met Ser Asn Trp Tyr Trp Leu Tyr Gln Pro 
        35                  40                  45              
Thr Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln Glu Phe 
    50                  55                  60                  
Lys Glu Met Cys Ala Ala Ala Glu Glu Tyr Gly Ile Lys Val Ile Val 
65                  70                  75                  80  
Asp Ala Val Ile Asn His Thr Thr Ser Asp Tyr Ala Val Ile Ser Asn 
                85                  90                  95      
Glu Ile Lys Ser Ile Pro Asn Trp Thr His Gly Asn Thr Gln Ile Lys 
            100                 105                 110         
Asn Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu Gly Leu 
        115                 120                 125             
Tyr Asp Trp Asn Thr Gln Asn Thr Gln Val Gln Ser Tyr Leu Lys Arg 
    130                 135                 140                 
Phe Leu Glu Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg Phe Asp 
145                 150                 155                 160 
Ala Ala Lys His Ile Glu Leu Pro Asp Asp Gly Ser Tyr Gly Ser Gln 
                165                 170                 175     
Phe Trp Pro Asn Ile 
            180     

<210> 4
<211> 188
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtitlis

<400> 4
Asp Pro Asn Gln Lys Val Ile Ala Leu Thr Phe Asp Asp Gly Pro Asn 
1               5                   10                  15      
Pro Ala Thr Thr Asn Gln Ile Leu Asp Ser Leu Lys Lys Tyr Lys Gly 
            20                  25                  30          
His Ala Thr Phe Phe Val Leu Gly Ser Arg Val Gln Tyr Tyr Pro Glu 
        35                  40                  45              
Thr Leu Ile Arg Met Leu Lys Glu Gly Asn Glu Val Gly Asn His Ser 
    50                  55                  60                  
Trp Ser His Pro Leu Leu Thr Arg Leu Ser Val Lys Glu Ala Leu Lys 
65                  70                  75                  80  
Gln Ile Asn Asp Thr Gln Asp Ile Ile Glu Lys Ile Ser Gly Tyr Arg 
                85                  90                  95      
Pro Thr Leu Val Arg Pro Pro Tyr Gly Gly Ile Asn Asp Glu Leu Arg 
            100                 105                 110         
Ser Gln Met Lys Met Asp Val Ala Leu Trp Asp Val Asp Pro Glu Asp 
        115                 120                 125             
Trp Lys Asp Arg Asn Lys Lys Thr Ile Val Asp Arg Val Met Asn Gln 
    130                 135                 140                 
Ala Gly Asp Gly Arg Thr Ile Leu Ile His Asp Ile Tyr Arg Thr Ser 
145                 150                 155                 160 
Ala Asp Ala Ala Asp Glu Ile Ile Lys Lys Leu Thr Asp Gln Gly Tyr 
                165                 170                 175     
Gln Leu Val Thr Val Ser Gln Leu Glu Glu Val Lys 
            180                 185             

<210> 5
<211> 181
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 5
Ala Glu His Asn Pro Val Val Met Val His Gly Ile Gly Gly Ala Ser 
1               5                   10                  15      
Phe Asn Phe Ala Gly Ile Lys Ser Tyr Leu Val Ser Gln Gly Trp Ser 
            20                  25                  30          
Arg Asp Lys Leu Tyr Ala Val Asp Phe Trp Asp Lys Thr Gly Thr Asn 
        35                  40                  45              
Tyr Asn Asn Gly Pro Val Leu Ser Arg Phe Val Gln Lys Val Leu Asp 
    50                  55                  60                  
Glu Thr Gly Ala Lys Lys Val Asp Ile Val Ala His Ser Met Gly Gly 
65                  70                  75                  80  
Ala Asn Thr Leu Tyr Tyr Ile Lys Asn Leu Asp Gly Gly Asn Lys Val 
                85                  90                  95      
Ala Asn Val Val Thr Leu Gly Gly Ala Asn Arg Leu Thr Thr Gly Lys 
            100                 105                 110         
Ala Leu Pro Gly Thr Asp Pro Asn Gln Lys Ile Leu Tyr Thr Ser Ile 
        115                 120                 125             
Tyr Ser Ser Ala Asp Met Ile Val Met Asn Tyr Leu Ser Arg Leu Asp 
    130                 135                 140                 
Gly Ala Arg Asn Val Gln Ile His Gly Val Gly His Ile Gly Leu Leu 
145                 150                 155                 160 
Tyr Ser Ser Gln Val Asn Ser Leu Ile Lys Glu Gly Leu Asn Gly Gly 
                165                 170                 175     
Gly Gln Asn Thr Asn 
            180     

<210> 6
<211> 182
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 6
Glu Ser Val His Asn Pro Val Val Leu Val His Gly Ile Ser Gly Ala 
1               5                   10                  15      
Ser Tyr Asn Phe Phe Ala Ile Lys Asn Tyr Leu Ile Ser Gln Gly Trp 
            20                  25                  30          
Gln Ser Asn Lys Leu Tyr Ala Ile Asp Phe Tyr Asp Lys Thr Gly Asn 
        35                  40                  45              
Asn Leu Asn Asn Gly Pro Gln Leu Ala Ser Tyr Val Asp Arg Val Leu 
    50                  55                  60                  
Lys Glu Thr Gly Ala Lys Lys Val Asp Ile Val Ala His Ser Met Gly 
65                  70                  75                  80  
Gly Ala Asn Thr Leu Tyr Tyr Ile Lys Tyr Leu Gly Gly Gly Asn Lys 
                85                  90                  95      
Ile Gln Asn Val Val Thr Leu Gly Gly Ala Asn Gly Leu Val Ser Ser 
            100                 105                 110         
Thr Ala Leu Pro Gly Thr Asp Pro Asn Gln Lys Ile Leu Tyr Thr Ser 
        115                 120                 125             
Ile Tyr Ser Leu Asn Asp Gln Ile Val Ile Asn Ser Leu Ser Arg Leu 
    130                 135                 140                 
Gln Gly Ala Arg Asn Ile Gln Leu Tyr Gly Ile Gly His Ile Gly Leu 
145                 150                 155                 160 
Leu Ser Asn Ser Gln Val Asn Gly Tyr Ile Lys Glu Gly Leu Asn Gly 
                165                 170                 175     
Gly Gly Leu Asn Thr Asn 
            180         

<210> 7
<211> 275
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 7
Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      
His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          
Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         
Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Thr Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 
Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Thr Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     
Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Ala 
            180                 185                 190         
Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             
Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 
Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     
Leu Gly Asn Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ala Gln 
        275 

<210> 8
<211> 618
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 8
Leu Thr Ala Pro Ser Ile Lys Ser Gly Thr Ile Leu His Ala Trp Asn 
1               5                   10                  15      
Trp Ser Phe Asn Thr Leu Lys His Asn Met Lys Asp Ile His Asp Ala 
            20                  25                  30          
Gly Tyr Thr Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys Glu Gly 
        35                  40                  45              
Asn Gln Gly Asp Lys Ser Met Ser Asn Trp Tyr Trp Leu Tyr Gln Pro 
    50                  55                  60                  
Thr Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln Glu Phe 
65                  70                  75                  80  
Lys Glu Met Cys Ala Ala Ala Glu Glu Tyr Gly Ile Lys Val Ile Val 
                85                  90                  95      
Asp Ala Val Ile Asn His Thr Thr Ser Asp Tyr Ala Ala Ile Ser Asn 
            100                 105                 110         
Glu Val Lys Ser Ile Pro Asn Trp Thr His Gly Asn Thr Gln Ile Lys 
        115                 120                 125             
Asn Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu Gly Leu 
    130                 135                 140                 
Tyr Asp Trp Asn Thr Gln Asn Thr Gln Val Gln Ser Tyr Leu Lys Arg 
145                 150                 155                 160 
Phe Leu Asp Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg Phe Asp 
                165                 170                 175     
Ala Ala Lys His Ile Glu Leu Pro Asp Asp Gly Ser Tyr Gly Ser Gln 
            180                 185                 190         
Phe Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr Gly Glu 
        195                 200                 205             
Ile Leu Gln Asp Ser Ala Ser Arg Asp Ala Ala Tyr Ala Asn Tyr Met 
    210                 215                 220                 
Asp Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala Leu Lys 
225                 230                 235                 240 
Asn Arg Asn Leu Gly Val Ser Asn Ile Ser His Tyr Ala Ser Asp Val 
                245                 250                 255     
Ser Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr Tyr Ala 
            260                 265                 270         
Asn Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile Arg Leu 
        275                 280                 285             
Gly Trp Ala Val Ile Ala Ser Arg Ser Gly Ser Thr Pro Leu Phe Phe 
    290                 295                 300                 
Ser Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly Lys Ser 
305                 310                 315                 320 
Gln Ile Gly Asp Arg Gly Ser Ala Leu Phe Glu Asp Gln Ala Ile Thr 
                325                 330                 335     
Ala Val Asn Arg Phe His Asn Val Met Ala Gly Gln Pro Glu Glu Leu 
            340                 345                 350         
Ser Asn Pro Asn Gly Asn Asn Gln Ile Phe Met Asn Gln Arg Gly Ser 
        355                 360                 365             
His Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Ser Ile Asn 
    370                 375                 380                 
Thr Ala Thr Lys Leu Pro Asp Gly Arg Tyr Asp Asn Lys Ala Gly Ala 
385                 390                 395                 400 
Gly Ser Phe Gln Val Asn Asp Gly Lys Leu Thr Gly Thr Ile Asn Ala 
                405                 410                 415     
Arg Ser Val Ala Val Leu Tyr Pro Asp Asp Ile Ala Lys Ala Pro His 
            420                 425                 430         
Val Phe Leu Glu Asn Tyr Lys Thr Gly Val Thr His Ser Phe Asn Asp 
        435                 440                 445             
Gln Leu Thr Ile Thr Leu Arg Ala Asp Ala Asn Thr Thr Lys Ala Val 
    450                 455                 460                 
Tyr Gln Ile Asn Asn Gly Pro Glu Thr Ala Phe Lys Asp Gly Asp Gln 
465                 470                 475                 480 
Phe Thr Ile Gly Lys Gly Asp Pro Phe Gly Lys Thr Tyr Thr Ile Met 
                485                 490                 495     
Leu Lys Gly Thr Asn Ser Asp Gly Val Thr Arg Thr Glu Lys Tyr Ser 
            500                 505                 510         
Phe Val Lys Arg Asp Pro Ala Ser Ala Lys Thr Ile Gly Tyr Gln Asn 
        515                 520                 525             
Pro Asn His Trp Ser Gln Val Asn Ala Tyr Ile Tyr Lys His Asp Gly 
    530                 535                 540                 
Ser Arg Val Ile Glu Leu Thr Gly Ser Trp Pro Gly Lys Pro Met Thr 
545                 550                 555                 560 
Lys Asn Ala Asp Gly Ile Tyr Thr Leu Thr Leu Pro Ala Asp Thr Asp 
                565                 570                 575     
Thr Thr Asn Ala Lys Val Ile Phe Asn Asn Gly Ser Ala Gln Val Pro 
            580                 585                 590         
Gly Gln Asn Gln Pro Gly Phe Asp Tyr Val Leu Asn Gly Leu Tyr Asn 
        595                 600                 605             
Asp Ser Gly Leu Ser Gly Ser Leu Pro His 
    610                 615             

<210> 9
<211> 483
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 9
Ala Asn Leu Lys Gly Thr Leu Met Gln Tyr Phe Glu Trp Tyr Met Pro 
1               5                   10                  15      
Asn Asp Gly Gln His Trp Lys Arg Leu Gln Asn Asp Ser Ala Tyr Leu 
            20                  25                  30          
Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              
Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  
Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  
Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His Ser Arg Asp Ile Asn 
                85                  90                  95      
Val Tyr Gly Asp Val Val Ile Asn His Lys Gly Gly Ala Asp Ala Thr 
            100                 105                 110         
Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala Asp Arg Asn Arg Val 
        115                 120                 125             
Ile Ser Gly Glu His Arg Ile Lys Ala Trp Thr His Phe His Phe Pro 
    130                 135                 140                 
Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp His Trp Tyr His Phe 
145                 150                 155                 160 
Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile Tyr Lys 
                165                 170                 175     
Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser Asn Glu Asn Gly Asn 
            180                 185                 190         
Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             
Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr Ala Asn Glu Leu Gln 
    210                 215                 220                 
Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe 
225                 230                 235                 240 
Leu Arg Asp Trp Val Asn His Val Arg Glu Lys Thr Gly Lys Glu Met 
                245                 250                 255     
Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Glu Asn 
            260                 265                 270         
Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val Phe Asp Val Pro Leu 
        275                 280                 285             
His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly Gly Gly Tyr Asp Met 
    290                 295                 300                 
Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys His Pro Leu Lys Ala 
305                 310                 315                 320 
Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     
Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         
Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             
Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala Leu Lys His Lys Ile 
    370                 375                 380                 
Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala Tyr Gly Ala Gln His 
385                 390                 395                 400 
Asp Tyr Phe Asp His His Asp Ile Val Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     
Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         
Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln Asn Ala Gly Glu Thr 
        435                 440                 445             
Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro Val Val Ile Asn Ser 
    450                 455                 460                 
Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly Ser Val Ser Ile Tyr 
465                 470                 475                 480 
Val Gln Arg 
            

<210> 10
<211> 301
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. subtilis

<400> 10
His Ala Ala Ala Thr Gly Ser Gly Thr Thr Leu Lys Gly Ala Thr Val 
1               5                   10                  15      
Pro Leu Asn Ile Ser Tyr Glu Gly Gly Lys Tyr Val Leu Arg Asp Leu 
            20                  25                  30          
Ser Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg 
        35                  40                  45              
Gln Ser Arg Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Lys Thr Phe 
    50                  55                  60                  
Thr Ser Ser Ser Gln Arg Ala Ala Val Asp Ala His Tyr Asn Leu Gly 
65                  70                  75                  80  
Lys Val Tyr Asp Tyr Phe Tyr Ser Asn Phe Lys Arg Asn Ser Tyr Asp 
                85                  90                  95      
Asn Lys Gly Ser Lys Ile Val Ser Ser Val His Tyr Gly Thr Gln Tyr 
            100                 105                 110         
Asn Asn Ala Ala Trp Thr Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp 
        115                 120                 125             
Gly Ser Phe Phe Ser Pro Leu Ser Gly Ser Leu Asp Val Thr Ala His 
    130                 135                 140                 
Glu Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu Ile Tyr Glu 
145                 150                 155                 160 
Asn Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr 
                165                 170                 175     
Phe Asn Asp Thr Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Val Ser 
            180                 185                 190         
Gln Pro Ala Leu Arg Ser Leu Ser Asn Pro Thr Lys Tyr Asn Gln Pro 
        195                 200                 205             
Asp Asn Tyr Ala Asn Tyr Arg Asn Leu Pro Asn Thr Asp Glu Gly Asp 
    210                 215                 220                 
Tyr Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr 
225                 230                 235                 240 
Asn Thr Ile Thr Lys Leu Gly Val Ser Lys Ser Gln Gln Ile Tyr Tyr 
                245                 250                 255     
Arg Ala Leu Thr Thr Tyr Leu Thr Pro Ser Ser Thr Phe Lys Asp Ala 
            260                 265                 270         
Lys Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Thr Asp 
        275                 280                 285             
Ala Ala Lys Val Glu Ala Ala Trp Asn Ala Val Gly Leu 
    290                 295                 300     

<210> 11
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human

<400> 11
Lys Gln Phe Thr Lys Cys Glu Leu Ser Gln Leu Leu Lys Asp Ile Asp 
1               5                   10                  15      
Gly Tyr Gly Gly Ile Ala Leu Pro Glu Leu Ile Cys Thr Met Phe His 
            20                  25                  30          
Thr Ser Gly Tyr Asp Thr Gln Ala Ile Val Glu Asn Asn Glu Ser Thr 
        35                  40                  45              
Glu Tyr Gly Leu Phe Gln Ile Ser Asn Lys Leu Trp Cys Lys Ser Ser 
    50                  55                  60                  
Gln Val Pro Gln Ser Arg Asn Ile Cys Asp Ile Ser Cys Asp Lys Phe 
65                  70                  75                  80  
Leu Asp Asp Asp Ile Thr Asp Asp Ile Met Cys Ala Lys Lys Ile Leu 
                85                  90                  95      
Asp Ile Lys Gly Ile Asp Tyr Trp Leu Ala His Lys Ala Leu Cys Thr 
            100                 105                 110         
Glu Lys Leu Glu Gln Trp Leu Cys Glu Lys Leu 
        115                 120             

<210> 12
<211> 274
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. licheniformis

<400> 12
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Asn Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Phe Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln 
        

<210> 13
<211> 174
<212> PRT
<213> Artificial Sequence

<220> 
<223> B. licheniformis

<400> 13
Ala Ser His Asn Pro Val Val Met Val His Gly Ile Gly Gly Ala Asp 
1               5                   10                  15      
Tyr Asn Phe Ile Gly Ile Lys Ser Tyr Leu Gln Ser Gln Gly Trp Thr 
            20                  25                  30          
Ser Ser Glu Leu Tyr Ala Ile Asn Phe Ile Asp Lys Thr Gly Asn Asn 
        35                  40                  45              
Ile Asn Asn Ala Pro Arg Leu Ser Glu Tyr Ile Lys Arg Val Leu Asn 
    50                  55                  60                  
Gln Thr Gly Ala Ser Lys Val Asp Ile Val Ala His Ser Met Gly Gly 
65                  70                  75                  80  
Ala Asn Thr Leu Tyr Tyr Ile Lys Asn Leu Asp Gly Ala Asp Lys Val 
                85                  90                  95      
Gly His Val Val Thr Leu Gly Gly Ala Asn Arg Leu Val Thr Asn Thr 
            100                 105                 110         
Ala Pro Gln Asn Asp Lys Ile Ser Tyr Thr Ser Ile Tyr Ser Thr Ser 
        115                 120                 125             
Asp Tyr Ile Val Leu Asn Ser Leu Ser Lys Leu Asp Gly Ala Asn Asn 
    130                 135                 140                 
Val Gln Ile Ser Gly Val Ser His Val Gly Leu Leu Phe Ser Ser Lys 
145                 150                 155                 160 
Val Asn Ala Leu Ile Lys Asp Gly Leu Thr Ala Ser Gly Lys 
                165                 170                 

<210> 14
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> EGFP

<400> 14
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 15
<211> 7956
<212> DNA
<213> Artificial Sequence

<220> 
<223> pHT01

<400> 15
ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa       60

tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa      120

gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat      180

aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt      240

tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat      300

ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag      360

gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt      420

ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt      480

tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc      540

cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga      600

aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa      660

tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct      720

cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa      780

tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc      840

ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat      900

ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa      960

aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc     1020

ttatcttgat aataagggta actattgccg atcgtccatt ccgacagcat cgccagtcac     1080

tatggcgtgc tgctagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc     1140

ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt     1200

aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt     1260

cgagctcagg ccttaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     1320

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     1380

tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct     1440

tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc     1500

gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt     1560

cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca     1620

ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat     1680

tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg     1740

ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg     1800

ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca     1860

gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg     1920

tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa     1980

tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa     2040

gattgtgcac cgccgtttta caggcttcga cgccgcttcg ttctaccatc gacaccacca     2100

cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt     2160

gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt     2220

gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg     2280

ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac     2340

cggcatactc tgcgacatcg tataacgtta ctggtttcat caaaatcgtc tccctccgtt     2400

tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat     2460

ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc     2520

aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt     2580

tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca gctattgtaa     2640

cataatcggt acgggggtga aaaagctaac ggaaaaggga gcggaaaaga atgatgtaag     2700

cgtgaaaaat tttttatctt atcacttgaa attggaaggg agattcttta ttataagaat     2760

tgtggaattg tgagcggata acaattccca attaaaggag gaaggatcct ctagagtcga     2820

cgtccccggg gcagcccgcc taatgagcgg gcttttttca cgtcacgcgt ccatggagat     2880

ctttgtctgc aactgaaaag tttatacctt acctggaaca aatggttgaa acatacgagg     2940

ctaatatcgg cttattagga atagtccctg tactaataaa atcaggtgga tcagttgatc     3000

agtatatttt ggacgaagct cggaaagaat ttggagatga cttgcttaat tccacaatta     3060

aattaaggga aagaataaag cgatttgatg ttcaaggaat cacggaagaa gatactcatg     3120

ataaagaagc tctaaaacta ttcaataacc ttacaatgga attgatcgaa agggtggaag     3180

gttaatggta cgaaaattag gggatctacc tagaaagcca caaggcgata ggtcaagctt     3240

aaagaaccct tacatggatc ttacagattc tgaaagtaaa gaaacaacag aggttaaaca     3300

aacagaacca aaaagaaaaa aagcattgtt gaaaacaatg aaagttgatg tttcaatcca     3360

taataagatt aaatcgctgc acgaaattct ggcagcatcc gaagggaatt catattactt     3420

agaggatact attgagagag ctattgataa gatggttgag acattacctg agagccaaaa     3480

aactttttat gaatatgaat taaaaaaaag aaccaacaaa ggctgagaca gactccaaac     3540

gagtctgttt ttttaaaaaa aatattagga gcattgaata tatattagag aattaagaaa     3600

gacatgggaa taaaaatatt ttaaatccag taaaaatatg ataagattat ttcagaatat     3660

gaagaactct gtttgttttt gatgaaaaaa caaacaaaaa aaatccacct aacggaatct     3720

caatttaact aacagcggcc aaactgagaa gttaaatttg agaaggggaa aaggcggatt     3780

tatacttgta tttaactatc tccattttaa cattttatta aaccccatac aagtgaaaat     3840

cctcttttac actgttcctt taggtgatcg cggagggaca ttatgagtga agtaaaccta     3900

aaaggaaata cagatgaatt agtgtattat cgacagcaaa ccactggaaa taaaatcgcc     3960

aggaagagaa tcaaaaaagg gaaagaagaa gtttattatg ttgctgaaac ggaagagaag     4020

atatggacag aagagcaaat aaaaaacttt tctttagaca aatttggtac gcatatacct     4080

tacatagaag gtcattatac aatcttaaat aattacttct ttgatttttg gggctatttt     4140

ttaggtgctg aaggaattgc gctctatgct cacctaactc gttatgcata cggcagcaaa     4200

gacttttgct ttcctagtct acaaacaatc gctaaaaaaa tggacaagac tcctgttaca     4260

gttagaggct acttgaaact gcttgaaagg tacggtttta tttggaaggt aaacgtccgt     4320

aataaaacca aggataacac agaggaatcc ccgattttta agattagacg taaggttcct     4380

ttgctttcag aagaactttt aaatggaaac cctaatattg aaattccaga tgacgaggaa     4440

gcacatgtaa agaaggcttt aaaaaaggaa aaagagggtc ttccaaaggt tttgaaaaaa     4500

gagcacgatg aatttgttaa aaaaatgatg gatgagtcag aaacaattaa tattccagag     4560

gccttacaat atgacacaat gtatgaagat atactcagta aaggagaaat tcgaaaagaa     4620

atcaaaaaac aaatacctaa tcctacaaca tcttttgaga gtatatcaat gacaactgaa     4680

gaggaaaaag tcgacagtac tttaaaaagc gaaatgcaaa atcgtgtctc taagccttct     4740

tttgatacct ggtttaaaaa cactaagatc aaaattgaaa ataaaaattg tttattactt     4800

gtaccgagtg aatttgcatt tgaatggatt aagaaaagat atttagaaac aattaaaaca     4860

gtccttgaag aagctggata tgttttcgaa aaaatcgaac taagaaaagt gcaataaact     4920

gctgaagtat ttcagcagtt ttttttattt agaaatagtg aaaaaaatat aatcagggag     4980

gtatcaatat ttaatgagta ctgatttaaa tttatttaga ctggaattaa taattaacac     5040

gtagactaat taaaatttaa tgagggataa agaggataca aaaatattaa tttcaatccc     5100

tattaaattt taacaagggg gggattaaaa tttaattaga ggtttatcca caagaaaaga     5160

ccctaataaa atttttacta gggttataac actgattaat ttcttaatgg gggagggatt     5220

aaaatttaat gacaaagaaa acaatctttt aagaaaagct tttaaaagat aataataaaa     5280

agagctttgc gattaagcaa aactctttac tttttcattg acattatcaa attcatcgat     5340

ttcaaattgt tgttgtatca taaagttaat tctgttttgc acaacctttt caggaatata     5400

aaacacatct gaggcttgtt ttataaactc agggtcgcta aagtcaatgt aacgtagcat     5460

atgatatggt atagcttcca cccaagttag cctttctgct tcttctgaat gtttttcata     5520

tacttccatg ggtatctcta aatgattttc ctcatgtagc aaggtatgag caaaaagttt     5580

atggaattga tagttcctct ctttttcttc aactttttta tctaaaacaa acactttaac     5640

atctgagtca atgtaagcat aagatgtttt tccagtcata atttcaatcc caaatctttt     5700

agacagaaat tctggacgta aatcttttgg tgaaagaatt tttttatgta gcaatatatc     5760

cgatacagca ccttctaaaa gcgttggtga atagggcatt ttacctatct cctctcattt     5820

tgtggaataa aaatagtcat attcgtccat ctacctatcc tattatcgaa cagttgaact     5880

ttttaatcaa ggatcagtcc tttttttcat tattcttaaa ctgtgctctt aactttaaca     5940

actcgatttg tttttccaga tctcgagggt aactagcctc gccgatcccg caagaggccc     6000

ggcagtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     6060

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     6120

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     6180

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     6240

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     6300

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     6360

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact     6420

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     6480

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     6540

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     6600

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     6660

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg     6720

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     6780

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     6840

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     6900

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     6960

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     7020

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     7080

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     7140

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     7200

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     7260

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     7320

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     7380

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     7440

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     7500

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     7560

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     7620

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     7680

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     7740

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     7800

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     7860

ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag     7920

tgagcgagga agcggaagag cgcccaatac gcatgc                               7956


<210> 16
<211> 8057
<212> DNA
<213> Artificial Sequence

<220> 
<223> pHT43

<400> 16
ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa       60

tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa      120

gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat      180

aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt      240

tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat      300

ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag      360

gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt      420

ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt      480

tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc      540

cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga      600

aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa      660

tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct      720

cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa      780

tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc      840

ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat      900

ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa      960

aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct     1020

tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc gccagtcact     1080

atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg     1140

gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta     1200

agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc     1260

gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     1320

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     1380

attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt     1440

caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg     1500

aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt cggtatcgtc     1560

gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat     1620

tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt     1680

cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc     1740

tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc     1800

cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag     1860

atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt     1920

ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat     1980

ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag     2040

attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac     2100

gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg     2160

cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg     2220

tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt     2280

tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc     2340

ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct ccctccgttt     2400

gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta cagtgttatg     2460

gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac tcaaacatca     2520

aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg caaccgtttt     2580

ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag ctattgtaac     2640

ataatcggta cgggggtgaa aaagctaacg gaaaagggag cggaaaagaa tgatgtaagc     2700

gtgaaaaatt ttttatctta tcacttgaaa ttggaaggga gattctttat tataagaatt     2760

gtggaattgt gagcggataa caattcccaa ttaaaggagg aaggatcaat gattcaaaaa     2820

cgaaagcgga cagtttcgtt cagacttgtg cttatgtgca cgctgttatt tgtcagtttg     2880

ccgattacaa aaacatcagc cgtaggatcc tctagagtcg acgtccccgg ggcagcccgc     2940

ctaatgagcg ggcttttttc acgtcacgcg tccatggaga tctttgtctg caactgaaaa     3000

gtttatacct tacctggaac aaatggttga aacatacgag gctaatatcg gcttattagg     3060

aatagtccct gtactaataa aatcaggtgg atcagttgat cagtatattt tggacgaagc     3120

tcggaaagaa tttggagatg acttgcttaa ttccacaatt aaattaaggg aaagaataaa     3180

gcgatttgat gttcaaggaa tcacggaaga agatactcat gataaagaag ctctaaaact     3240

attcaataac cttacaatgg aattgatcga aagggtggaa ggttaatggt acgaaaatta     3300

ggggatctac ctagaaagcc acaaggcgat aggtcaagct taaagaaccc ttacatggat     3360

cttacagatt ctgaaagtaa agaaacaaca gaggttaaac aaacagaacc aaaaagaaaa     3420

aaagcattgt tgaaaacaat gaaagttgat gtttcaatcc ataataagat taaatcgctg     3480

cacgaaattc tggcagcatc cgaagggaat tcatattact tagaggatac tattgagaga     3540

gctattgata agatggttga gacattacct gagagccaaa aaacttttta tgaatatgaa     3600

ttaaaaaaaa gaaccaacaa aggctgagac agactccaaa cgagtctgtt tttttaaaaa     3660

aaatattagg agcattgaat atatattaga gaattaagaa agacatggga ataaaaatat     3720

tttaaatcca gtaaaaatat gataagatta tttcagaata tgaagaactc tgtttgtttt     3780

tgatgaaaaa acaaacaaaa aaaatccacc taacggaatc tcaatttaac taacagcggc     3840

caaactgaga agttaaattt gagaagggga aaaggcggat ttatacttgt atttaactat     3900

ctccatttta acattttatt aaaccccata caagtgaaaa tcctctttta cactgttcct     3960

ttaggtgatc gcggagggac attatgagtg aagtaaacct aaaaggaaat acagatgaat     4020

tagtgtatta tcgacagcaa accactggaa ataaaatcgc caggaagaga atcaaaaaag     4080

ggaaagaaga agtttattat gttgctgaaa cggaagagaa gatatggaca gaagagcaaa     4140

taaaaaactt ttctttagac aaatttggta cgcatatacc ttacatagaa ggtcattata     4200

caatcttaaa taattacttc tttgattttt ggggctattt tttaggtgct gaaggaattg     4260

cgctctatgc tcacctaact cgttatgcat acggcagcaa agacttttgc tttcctagtc     4320

tacaaacaat cgctaaaaaa atggacaaga ctcctgttac agttagaggc tacttgaaac     4380

tgcttgaaag gtacggtttt atttggaagg taaacgtccg taataaaacc aaggataaca     4440

cagaggaatc cccgattttt aagattagac gtaaggttcc tttgctttca gaagaacttt     4500

taaatggaaa ccctaatatt gaaattccag atgacgagga agcacatgta aagaaggctt     4560

taaaaaagga aaaagagggt cttccaaagg ttttgaaaaa agagcacgat gaatttgtta     4620

aaaaaatgat ggatgagtca gaaacaatta atattccaga ggccttacaa tatgacacaa     4680

tgtatgaaga tatactcagt aaaggagaaa ttcgaaaaga aatcaaaaaa caaataccta     4740

atcctacaac atcttttgag agtatatcaa tgacaactga agaggaaaaa gtcgacagta     4800

ctttaaaaag cgaaatgcaa aatcgtgtct ctaagccttc ttttgatacc tggtttaaaa     4860

acactaagat caaaattgaa aataaaaatt gtttattact tgtaccgagt gaatttgcat     4920

ttgaatggat taagaaaaga tatttagaaa caattaaaac agtccttgaa gaagctggat     4980

atgttttcga aaaaatcgaa ctaagaaaag tgcaataaac tgctgaagta tttcagcagt     5040

tttttttatt tagaaatagt gaaaaaaata taatcaggga ggtatcaata tttaatgagt     5100

actgatttaa atttatttag actggaatta ataattaaca cgtagactaa ttaaaattta     5160

atgagggata aagaggatac aaaaatatta atttcaatcc ctattaaatt ttaacaaggg     5220

ggggattaaa atttaattag aggtttatcc acaagaaaag accctaataa aatttttact     5280

agggttataa cactgattaa tttcttaatg ggggagggat taaaatttaa tgacaaagaa     5340

aacaatcttt taagaaaagc ttttaaaaga taataataaa aagagctttg cgattaagca     5400

aaactcttta ctttttcatt gacattatca aattcatcga tttcaaattg ttgttgtatc     5460

ataaagttaa ttctgttttg cacaaccttt tcaggaatat aaaacacatc tgaggcttgt     5520

tttataaact cagggtcgct aaagtcaatg taacgtagca tatgatatgg tatagcttcc     5580

acccaagtta gcctttctgc ttcttctgaa tgtttttcat atacttccat gggtatctct     5640

aaatgatttt cctcatgtag caaggtatga gcaaaaagtt tatggaattg atagttcctc     5700

tctttttctt caactttttt atctaaaaca aacactttaa catctgagtc aatgtaagca     5760

taagatgttt ttccagtcat aatttcaatc ccaaatcttt tagacagaaa ttctggacgt     5820

aaatcttttg gtgaaagaat ttttttatgt agcaatatat ccgatacagc accttctaaa     5880

agcgttggtg aatagggcat tttacctatc tcctctcatt ttgtggaata aaaatagtca     5940

tattcgtcca tctacctatc ctattatcga acagttgaac tttttaatca aggatcagtc     6000

ctttttttca ttattcttaa actgtgctct taactttaac aactcgattt gtttttccag     6060

atctcgaggg taactagcct cgccgatccc gcaagaggcc cggcagtcag gtggcacttt     6120

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta     6180

tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat     6240

gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt     6300

ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg     6360

agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga     6420

agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg     6480

tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt     6540

tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg     6600

cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg     6660

aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga     6720

tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc     6780

tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc     6840

ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc     6900

ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg     6960

cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac     7020

gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc     7080

actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt     7140

aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac     7200

caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa     7260

aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc     7320

accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt     7380

aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg     7440

ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc     7500

agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt     7560

accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga     7620

gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct     7680

tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg     7740

cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca     7800

cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa     7860

cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt     7920

ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga     7980

taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga     8040

gcgcccaata cgcatgc                                                    8057


<210> 17
<211> 131
<212> DNA
<213> Artificial Sequence

<220> 
<223> P100

<400> 17
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tattataaga attgcggaat tgtgagcgga taacaattcc catataaagg    120

aggaaggatc c                                                         131


<210> 18
<211> 142
<212> DNA
<213> Artificial Sequence

<220> 
<223> P223

<400> 18
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tataataaga atttgtggat tgtgagcgga tcacaattcc acaaccaaca    120

ccaattaaag gaggaaggat cc                                             142


<210> 19
<211> 142
<212> DNA
<213> Artificial Sequence

<220> 
<223> P250

<400> 19
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tattataaga atttgtggat tgtgagcgga tcacaattcc acaaccaaca    120

ccaattaaag gaggaaggat cc                                             142


<210> 20
<211> 291
<212> DNA
<213> Artificial Sequence

<220> 
<223> P431

<400> 20
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tattataaga attgcggaat tgtgagcgga taacaattcc catataaagg    120

aggaaggatc aatgattcaa aaacgaaagc ggacagtttc gttcagactt gtgcttatgt    180

gcacgctgtt atttgtcagt ttgccgatta caaaaacatc agccgtagga tccatgtcta    240

gagtcgacgt cgctcatcac catcaccatc accatcacta acgtccccgg g             291


<210> 21
<211> 302
<212> DNA
<213> Artificial Sequence

<220> 
<223> P432

<400> 21
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tattataaga atttgtggat tgtgagcgga tcacaattcc acaaccaaca    120

ccaattaaag gaggaaggat caatgattca aaaacgaaag cggacagttt cgttcagact    180

tgtgcttatg tgcacgctgt tatttgtcag tttgccgatt acaaaaacat cagccgtagg    240

atccatgtct agagtcgacg tcgctcatca ccatcaccat caccatcact aacgtccccg    300

gg                                                                   302


<210> 22
<211> 302
<212> DNA
<213> Artificial Sequence

<220> 
<223> P433

<400> 22
ggtaccaaag gaggtaagga tcactagaaa attttttaaa aaatctcttg acattggaag     60

ggagatatgt tataataaga atttgtggat tgtgagcgga tcacaattcc acaaccaaca    120

ccaattaaag gaggaaggat caatgattca aaaacgaaag cggacagttt cgttcagact    180

tgtgcttatg tgcacgctgt tatttgtcag tttgccgatt acaaaaacat cagccgtagg    240

atccatgtct agagtcgacg tcgctcatca ccatcaccat caccatcact aacgtccccg    300

gg                                                                   302


<210> 23
<211> 93
<212> DNA
<213> Artificial Sequence

<220> 
<223> encoding the signal peptide of amyQ

<400> 23
atgattcaaa aacgaaagcg gacagtttcg ttcagacttg tgcttatgtg cacgctgtta     60

tttgtcagtt tgccgattac aaaaacatca gcc                                  93


<210> 24
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> encoding the native signal of aprE

<400> 24
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg     60

gcgttcagca acatgtctgc gcaggct                                         87


<210> 25
<211> 45
<212> PRT
<213> Bacteria <prokaryote>

<220> 
<223> PDB code 2B9K

<400> 25
Ala Ile Lys Leu Val Gln Ser Pro Asn Gly Asn Phe Ala Ala Ser Phe 
1               5                   10                  15      
Val Leu Asp Gly Thr Lys Trp Ile Phe Lys Tyr Tyr Asp Ser Ser Lys 
            20                  25                  30          
Gly Tyr Trp Val Gln Ile Tyr Glu Val Trp Asp Arg Lys 
        35                  40                  45  

<210> 26
<211> 275
<212> PRT
<213> Artificial Sequence

<220> 
<223> aprE50Y189Y261L

<400> 26
Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      
His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          
Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              
Ser Tyr Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         
Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Thr Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 
Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Thr Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     
Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Tyr Ser Ser Ala 
            180                 185                 190         
Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             
Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 
Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     
Leu Gly Asn Ser Leu Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ala Gln 
        275 

<210> 27
<211> 277
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 7: aprE50Y189Y261LCC

<400> 27
Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      
His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          
Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              
Ser Tyr Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         
Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Thr Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 
Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Thr Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     
Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Tyr Ser Ser Ala 
            180                 185                 190         
Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             
Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 
Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     
Leu Gly Asn Ser Leu Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ala Gln Cys Cys 
        275         

<210> 28
<211> 274
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 12: Blapr58Y188Y260W

<400> 28
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Tyr Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Asn Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Tyr Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Trp Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln 
        

<210> 29
<211> 276
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 12: Blapr58Y188Y260WCC

<400> 29
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Tyr Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Asn Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Tyr Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Trp Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln Cys Cys 
        275     

<210> 30
<211> 274
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 12: Blapr58C188Y260W

<400> 30
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Cys Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Asn Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Tyr Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Trp Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln 
        

<210> 31
<211> 181
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 5: estA17Y19Y41C58Y

<400> 31
Ala Glu His Asn Pro Val Val Met Val His Gly Ile Gly Gly Ala Ser 
1               5                   10                  15      
Tyr Asn Tyr Ala Gly Ile Lys Ser Tyr Leu Val Ser Gln Gly Trp Ser 
            20                  25                  30          
Arg Asp Lys Leu Tyr Ala Val Asp Cys Trp Asp Lys Thr Gly Thr Asn 
        35                  40                  45              
Tyr Asn Asn Gly Pro Val Leu Ser Arg Tyr Val Gln Lys Val Leu Asp 
    50                  55                  60                  
Glu Thr Gly Ala Lys Lys Val Asp Ile Val Ala His Ser Met Gly Gly 
65                  70                  75                  80  
Ala Asn Thr Leu Tyr Tyr Ile Lys Asn Leu Asp Gly Gly Asn Lys Val 
                85                  90                  95      
Ala Asn Val Val Thr Leu Gly Gly Ala Asn Arg Leu Thr Thr Gly Lys 
            100                 105                 110         
Ala Leu Pro Gly Thr Asp Pro Asn Gln Lys Ile Leu Tyr Thr Ser Ile 
        115                 120                 125             
Tyr Ser Ser Ala Asp Met Ile Val Met Asn Tyr Leu Ser Arg Leu Asp 
    130                 135                 140                 
Gly Ala Arg Asn Val Gln Ile His Gly Val Gly His Ile Gly Leu Leu 
145                 150                 155                 160 
Tyr Ser Ser Gln Val Asn Ser Leu Ile Lys Glu Gly Leu Asn Gly Gly 
                165                 170                 175     
Gly Gln Asn Thr Asn 
            180     

<210> 32
<211> 181
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 5: estA17W19Y41C58Y

<400> 32
Ala Glu His Asn Pro Val Val Met Val His Gly Ile Gly Gly Ala Ser 
1               5                   10                  15      
Trp Asn Tyr Ala Gly Ile Lys Ser Tyr Leu Val Ser Gln Gly Trp Ser 
            20                  25                  30          
Arg Asp Lys Leu Tyr Ala Val Asp Cys Trp Asp Lys Thr Gly Thr Asn 
        35                  40                  45              
Tyr Asn Asn Gly Pro Val Leu Ser Arg Tyr Val Gln Lys Val Leu Asp 
    50                  55                  60                  
Glu Thr Gly Ala Lys Lys Val Asp Ile Val Ala His Ser Met Gly Gly 
65                  70                  75                  80  
Ala Asn Thr Leu Tyr Tyr Ile Lys Asn Leu Asp Gly Gly Asn Lys Val 
                85                  90                  95      
Ala Asn Val Val Thr Leu Gly Gly Ala Asn Arg Leu Thr Thr Gly Lys 
            100                 105                 110         
Ala Leu Pro Gly Thr Asp Pro Asn Gln Lys Ile Leu Tyr Thr Ser Ile 
        115                 120                 125             
Tyr Ser Ser Ala Asp Met Ile Val Met Asn Tyr Leu Ser Arg Leu Asp 
    130                 135                 140                 
Gly Ala Arg Asn Val Gln Ile His Gly Val Gly His Ile Gly Leu Leu 
145                 150                 155                 160 
Tyr Ser Ser Gln Val Asn Ser Leu Ile Lys Glu Gly Leu Asn Gly Gly 
                165                 170                 175     
Gly Gln Asn Thr Asn 
            180     

<210> 33
<211> 182
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 6: estB20Y21M40C

<400> 33
Glu Ser Val His Asn Pro Val Val Leu Val His Gly Ile Ser Gly Ala 
1               5                   10                  15      
Ser Tyr Asn Tyr Met Ala Ile Lys Asn Tyr Leu Ile Ser Gln Gly Trp 
            20                  25                  30          
Gln Ser Asn Lys Leu Tyr Ala Ile Asp Cys Tyr Asp Lys Thr Gly Asn 
        35                  40                  45              
Asn Leu Asn Asn Gly Pro Gln Leu Ala Ser Tyr Val Asp Arg Val Leu 
    50                  55                  60                  
Lys Glu Thr Gly Ala Lys Lys Val Asp Ile Val Ala His Ser Met Gly 
65                  70                  75                  80  
Gly Ala Asn Thr Leu Tyr Tyr Ile Lys Tyr Leu Gly Gly Gly Asn Lys 
                85                  90                  95      
Ile Gln Asn Val Val Thr Leu Gly Gly Ala Asn Gly Leu Val Ser Ser 
            100                 105                 110         
Thr Ala Leu Pro Gly Thr Asp Pro Asn Gln Lys Ile Leu Tyr Thr Ser 
        115                 120                 125             
Ile Tyr Ser Leu Asn Asp Gln Ile Val Ile Asn Ser Leu Ser Arg Leu 
    130                 135                 140                 
Gln Gly Ala Arg Asn Ile Gln Leu Tyr Gly Ile Gly His Ile Gly Leu 
145                 150                 155                 160 
Leu Ser Asn Ser Gln Val Asn Gly Tyr Ile Lys Glu Gly Leu Asn Gly 
                165                 170                 175     
Gly Gly Leu Asn Thr Asn 
            180         

<210> 34
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 11: ALAB-V4

<400> 34
Lys Gln Val Thr Lys Cys Glu Leu Ser Gln Leu Leu Lys Asp Ile Asp 
1               5                   10                  15      
Gly Tyr Gly Gly Ile Ala Leu Pro Glu Leu Ile Cys Thr Met Val His 
            20                  25                  30          
Thr Ser Gly Tyr Asp Thr Gln Ala Ile Val Glu Asn Asn Glu Ser Thr 
        35                  40                  45              
Glu Tyr Gly Leu Val Gln Ile Ser Asn Lys Leu Trp Cys Lys Ser Ser 
    50                  55                  60                  
Gln Val Pro Gln Ser Arg Asn Ile Cys Asp Ile Ser Cys Asp Lys Val 
65                  70                  75                  80  
Leu Asp Asp Asp Ile Thr Asp Asp Ile Met Cys Ala Lys Lys Ile Leu 
                85                  90                  95      
Asp Ile Lys Gly Ile Asp Tyr Trp Leu Ala His Lys Ala Leu Cys Thr 
            100                 105                 110         
Glu Lys Leu Glu Gln Trp Leu Cys Glu Lys Leu 
        115                 120             

<210> 35
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 11: ALAB-M1V3

<400> 35
Lys Gln Met Thr Lys Cys Glu Leu Ser Gln Leu Leu Lys Asp Ile Asp 
1               5                   10                  15      
Gly Tyr Gly Gly Ile Ala Leu Pro Glu Leu Ile Cys Thr Met Val His 
            20                  25                  30          
Thr Ser Gly Tyr Asp Thr Gln Ala Ile Val Glu Asn Asn Glu Ser Thr 
        35                  40                  45              
Glu Tyr Gly Leu Val Gln Ile Ser Asn Lys Leu Trp Cys Lys Ser Ser 
    50                  55                  60                  
Gln Val Pro Gln Ser Arg Asn Ile Cys Asp Ile Ser Cys Asp Lys Val 
65                  70                  75                  80  
Leu Asp Asp Asp Ile Thr Asp Asp Ile Met Cys Ala Lys Lys Ile Leu 
                85                  90                  95      
Asp Ile Lys Gly Ile Asp Tyr Trp Leu Ala His Lys Ala Leu Cys Thr 
            100                 105                 110         
Glu Lys Leu Glu Gln Trp Leu Cys Glu Lys Leu 
        115                 120             

<210> 36
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 11: ALAB-Y1V3

<400> 36
Lys Gln Met Thr Lys Cys Glu Leu Ser Gln Leu Leu Lys Asp Ile Asp 
1               5                   10                  15      
Gly Tyr Gly Gly Ile Ala Leu Pro Glu Leu Ile Cys Thr Met Val His 
            20                  25                  30          
Thr Ser Gly Tyr Asp Thr Gln Ala Ile Val Glu Asn Asn Glu Ser Thr 
        35                  40                  45              
Glu Tyr Gly Leu Val Gln Ile Ser Asn Lys Leu Trp Cys Lys Ser Ser 
    50                  55                  60                  
Gln Val Pro Gln Ser Arg Asn Ile Cys Asp Ile Ser Cys Asp Lys Val 
65                  70                  75                  80  
Leu Asp Asp Asp Ile Thr Asp Asp Ile Met Cys Ala Lys Lys Ile Leu 
                85                  90                  95      
Asp Ile Lys Gly Ile Asp Tyr Trp Leu Ala His Lys Ala Leu Cys Thr 
            100                 105                 110         
Glu Lys Leu Glu Gln Trp Leu Cys Glu Lys Leu 
        115                 120             

<210> 37
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutated protein of SEQ ID NO 11: ALAB-H1V3

<400> 37
Lys Gln His Thr Lys Cys Glu Leu Ser Gln Leu Leu Lys Asp Ile Asp 
1               5                   10                  15      
Gly Tyr Gly Gly Ile Ala Leu Pro Glu Leu Ile Cys Thr Met Val His 
            20                  25                  30          
Thr Ser Gly Tyr Asp Thr Gln Ala Ile Val Glu Asn Asn Glu Ser Thr 
        35                  40                  45              
Glu Tyr Gly Leu Val Gln Ile Ser Asn Lys Leu Trp Cys Lys Ser Ser 
    50                  55                  60                  
Gln Val Pro Gln Ser Arg Asn Ile Cys Asp Ile Ser Cys Asp Lys Val 
65                  70                  75                  80  
Leu Asp Asp Asp Ile Thr Asp Asp Ile Met Cys Ala Lys Lys Ile Leu 
                85                  90                  95      
Asp Ile Lys Gly Ile Asp Tyr Trp Leu Ala His Lys Ala Leu Cys Thr 
            100                 105                 110         
Glu Lys Leu Glu Gln Trp Leu Cys Glu Lys Leu 
        115                 120             

<210> 38
<211> 1146
<212> DNA
<213> Artificial Sequence

<220> 
<223> aprE50Y189Y261L

<400> 38
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg      60

gcgttcagca acatgtctgc gcaggctgcc ggaaaaagca gtacagaaaa gaaatacatt     120

gtcggattta aacagacaat gagtgccatg agttccgcca agaaaaagga tgttatttct     180

gaaaaaggcg gaaaggttca aaagcaattt aagtatgtta acgcggccgc agcaacattg     240

gatgaaaaag ctgtaaaaga attgaaaaaa gatccgagcg ttgcatatgt ggaagaagat     300

catattgcac atgaatatgc gcaatctgtt ccttatggca tttctcaaat taaagcgccg     360

gctcttcact ctcaaggcta cacaggctct aacgtaaaag tagctgttat cgacagcgga     420

attgactctt ctcatcctga cttaaacgtc agaggcggag caagctacgt accttctgaa     480

acaaacccat accaggacgg cagttctcac ggtacgcatg tagccggtac gattgccgct     540

cttaataact caatcggtgt tctgggcgta gcgccaagcg catcattata tgcagtaaaa     600

gtgcttgatt caacaggaag cggccaatat agctggatta ttaacggcat tgagtgggcc     660

atttccaaca atatggatgt tatcaacatg agccttggcg gacctactgg ttctacagcg     720

ctgaaaacag tcgttgacaa agccgtttcc agcggtatcg tcgttgctgc cgcagccgga     780

aacgaaggtt catccggaag cacaagcaca gtcggctacc ctgcaaaata tccttctact     840

attgcagtag gtgcggtaaa cagcagcaac caaagagctt catactccag cgcaggttct     900

gagcttgatg tgatggctcc tggcgtgtcc atccaaagca cacttcctgg aggcacttac     960

ggcgcttata acggaacgtc catggcgact cctcacgttg ccggagcagc agcgttaatt    1020

ctttctaagc acccgacttg gacaaacgcg caagtccgtg atcgtttaga aagcactgca    1080

acatatcttg gaaactctct ctactatgga aaagggttaa tcaacgtaca agcagctgca    1140

caataa                                                               1146


<210> 39
<211> 1152
<212> DNA
<213> Artificial Sequence

<220> 
<223> aprE50Y189Y261LCC

<400> 39
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg      60

gcgttcagca acatgtctgc gcaggctgcc ggaaaaagca gtacagaaaa gaaatacatt     120

gtcggattta aacagacaat gagtgccatg agttccgcca agaaaaagga tgttatttct     180

gaaaaaggcg gaaaggttca aaagcaattt aagtatgtta acgcggccgc agcaacattg     240

gatgaaaaag ctgtaaaaga attgaaaaaa gatccgagcg ttgcatatgt ggaagaagat     300

catattgcac atgaatatgc gcaatctgtt ccttatggca tttctcaaat taaagcgccg     360

gctcttcact ctcaaggcta cacaggctct aacgtaaaag tagctgttat cgacagcgga     420

attgactctt ctcatcctga cttaaacgtc agaggcggag caagctacgt accttctgaa     480

acaaacccat accaggacgg cagttctcac ggtacgcatg tagccggtac gattgccgct     540

cttaataact caatcggtgt tctgggcgta gcgccaagcg catcattata tgcagtaaaa     600

gtgcttgatt caacaggaag cggccaatat agctggatta ttaacggcat tgagtgggcc     660

atttccaaca atatggatgt tatcaacatg agccttggcg gacctactgg ttctacagcg     720

ctgaaaacag tcgttgacaa agccgtttcc agcggtatcg tcgttgctgc cgcagccgga     780

aacgaaggtt catccggaag cacaagcaca gtcggctacc ctgcaaaata tccttctact     840

attgcagtag gtgcggtaaa cagcagcaac caaagagctt catactccag cgcaggttct     900

gagcttgatg tgatggctcc tggcgtgtcc atccaaagca cacttcctgg aggcacttac     960

ggcgcttata acggaacgtc catggcgact cctcacgttg ccggagcagc agcgttaatt    1020

ctttctaagc acccgacttg gacaaacgcg caagtccgtg atcgtttaga aagcactgca    1080

acatatcttg gaaactctct ctactatgga aaagggttaa tcaacgtaca agcagctgca    1140

caatgttgct aa                                                        1152


<210> 40
<211> 1140
<212> DNA
<213> Artificial Sequence

<220> 
<223> Blapr58Y188Y260W

<400> 40
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttaatgct cgtgttcacg      60

atggccttca gcgattccgc gtctgctgct cagccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcggg agtgaaaacc gcatccgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttgaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcac     300

gtagctcatg ctttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctacaa gggagcgaac gtaaaagtcg ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gctacgtagc tggcgaagct     480

tataacaccg acggcaacgg acacggcacg catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgccg aacgtatcct tgtacgcggt taaagtgctg     600

aattcaagcg gaagcggatc ttacagcggc attgtaagcg gaatcgagtg ggcgacgaca     660

aacggcatgg atgttatcaa catgagcctt ggaggaccat caggctcaac agcgatgaaa     720

caggcggttg acaatgcata tgcaagaggg gttgtcgttg tggcggctgc tgggaacagc     780

ggatcttcag gaaacacgaa tacaatcggc tatcctgcga aatacgactc tgtcatcgca     840

gttggcgcgg tagactctaa cagcaacaga gcttcatatt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtgtac agcacttacc caaccagcac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagtac ggcgacttat    1080

ttgggaagct cctggtacta tggaaaaggt ctgatcaatg tcgaagctgc cgctcaataa    1140


<210> 41
<211> 1146
<212> DNA
<213> Artificial Sequence

<220> 
<223> Blapr58Y188Y260WCC

<400> 41
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttaatgct cgtgttcacg      60

atggccttca gcgattccgc gtctgctgct cagccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcggg agtgaaaacc gcatccgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttgaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcac     300

gtagctcatg ctttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctacaa gggagcgaac gtaaaagtcg ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gctacgtagc tggcgaagct     480

tataacaccg acggcaacgg acacggcacg catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgccg aacgtatcct tgtacgcggt taaagtgctg     600

aattcaagcg gaagcggatc ttacagcggc attgtaagcg gaatcgagtg ggcgacgaca     660

aacggcatgg atgttatcaa catgagcctt ggaggaccat caggctcaac agcgatgaaa     720

caggcggttg acaatgcata tgcaagaggg gttgtcgttg tggcggctgc tgggaacagc     780

ggatcttcag gaaacacgaa tacaatcggc tatcctgcga aatacgactc tgtcatcgca     840

gttggcgcgg tagactctaa cagcaacaga gcttcatatt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtgtac agcacttacc caaccagcac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagtac ggcgacttat    1080

ttgggaagct cctggtacta tggaaaaggt ctgatcaatg tcgaagctgc cgctcaatgt    1140

tgctaa                                                               1146


<210> 42
<211> 1140
<212> DNA
<213> Artificial Sequence

<220> 
<223> Blapr58C188Y260W

<400> 42
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttaatgct cgtgttcacg      60

atggccttca gcgattccgc gtctgctgct cagccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcggg agtgaaaacc gcatccgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttgaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcac     300

gtagctcatg ctttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctacaa gggagcgaac gtaaaagtcg ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gctgcgtagc tggcgaagct     480

tataacaccg acggcaacgg acacggcacg catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgccg aacgtatcct tgtacgcggt taaagtgctg     600

aattcaagcg gaagcggatc ttacagcggc attgtaagcg gaatcgagtg ggcgacgaca     660

aacggcatgg atgttatcaa catgagcctt ggaggaccat caggctcaac agcgatgaaa     720

caggcggttg acaatgcata tgcaagaggg gttgtcgttg tggcggctgc tgggaacagc     780

ggatcttcag gaaacacgaa tacaatcggc tatcctgcga aatacgactc tgtcatcgca     840

gttggcgcgg tagactctaa cagcaacaga gcttcatatt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtgtac agcacttacc caaccagcac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagtac ggcgacttat    1080

ttgggaagct cctggtacta tggaaaaggt ctgatcaatg tcgaagctgc cgctcaataa    1140


<210> 43
<211> 546
<212> DNA
<213> Artificial Sequence

<220> 
<223> estA17Y19Y41C58Y

<400> 43
gctgaacaca atccagtcgt tatggttcac ggtattggag gggcatcata caattatgcg      60

ggaattaaga gctatctcgt atctcagggc tggtcgcggg acaagctgta tgcagttgat     120

tgttgggaca agacaggcac aaattataac aatggaccgg tattatcacg atatgtgcaa     180

aaggttttag atgaaacggg tgcgaaaaaa gtggatattg tcgctcacag catggggggc     240

gcgaacacac tttactacat aaaaaatctg gacggcggaa ataaagttgc aaacgtcgtg     300

acgcttggcg gcgcgaaccg tttgacgaca ggcaaggcgc ttccgggaac agatccaaat     360

caaaagattt tatacacatc catttacagc agtgccgata tgattgtcat gaattactta     420

tcaagattag atggtgctag aaacgttcaa atccatggcg ttggacacat cggccttctg     480

tacagcagcc aagtcaacag cctgattaaa gaagggctga acggcggggg ccagaatacg     540

aattaa                                                                546


<210> 44
<211> 546
<212> DNA
<213> Artificial Sequence

<220> 
<223> estA17W19Y41C58Y

<400> 44
gctgaacaca atccagtcgt tatggttcac ggtattggag gggcatcatg gaattatgcg      60

ggaattaaga gctatctcgt atctcagggc tggtcgcggg acaagctgta tgcagttgat     120

tgttgggaca agacaggcac aaattataac aatggaccgg tattatcacg atatgtgcaa     180

aaggttttag atgaaacggg tgcgaaaaaa gtggatattg tcgctcacag catggggggc     240

gcgaacacac tttactacat aaaaaatctg gacggcggaa ataaagttgc aaacgtcgtg     300

acgcttggcg gcgcgaaccg tttgacgaca ggcaaggcgc ttccgggaac agatccaaat     360

caaaagattt tatacacatc catttacagc agtgccgata tgattgtcat gaattactta     420

tcaagattag atggtgctag aaacgttcaa atccatggcg ttggacacat cggccttctg     480

tacagcagcc aagtcaacag cctgattaaa gaagggctga acggcggggg ccagaatacg     540

aattaa                                                                546


<210> 45
<211> 549
<212> DNA
<213> Artificial Sequence

<220> 
<223> estB20Y21M40C

<400> 45
gagtcagtac ataatcctgt cgttcttgtt catggaataa gtggtgcatc atacaactat      60

atggctatta aaaactactt aatttctcaa ggctggcaaa gcaacaaact gtacgcaatt     120

gattgttatg ataaaacagg aaacaaccta aataacggcc cgcagcttgc ttcatatgtt     180

gaccgtgttt taaaagagac tggggcaaaa aaagtagata ttgtggctca tagtatggga     240

ggcgccaata cgctgtacta tattaaatat ttaggcgggg gcaataagat tcaaaatgtc     300

gtaacgcttg gaggggctaa tggtttagtg tcatcaaccg cgctgccggg cacagaccct     360

aatcaaaaga tcctctatac atctatttac agtctcaatg atcaaattgt catcaatagc     420

ttgtctcggt tacaaggagc gcgaaacatc cagctttatg gcatcggtca tattggcttg     480

ctttctaata gccaagtgaa cggctatatc aaagaagggc tgaatggcgg aggcctcaat     540

acaaattaa                                                             549


<210> 46
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> ALAB-V4

<400> 46
aagcaagtca caaaatgtga gctgtcccag ctgctgaaag acatagatgg ttatggaggc      60

atcgctttgc ctgaattgat ctgtaccatg gttcacacca gtggttatga cacacaagcc     120

atagttgaaa acaatgaaag cacggaatat ggactcgtcc agatcagtaa taagctttgg     180

tgcaagagca gccaggtccc tcagtcaagg aacatctgtg acatctcctg tgacaaggtc     240

ctggatgatg acattactga tgacataatg tgtgccaaga agatcctgga tattaaagga     300

attgactact ggttggccca taaagccctc tgcactgaga agctggaaca gtggctttgt     360

gagaagttgt ga                                                         372


<210> 47
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> ALAB-M1V3

<400> 47
aagcaaatga caaaatgtga gctgtcccag ctgctgaaag acatagatgg ttatggaggc      60

atcgctttgc ctgaattgat ctgtaccatg gttcacacca gtggttatga cacacaagcc     120

atagttgaaa acaatgaaag cacggaatat ggactcgtcc agatcagtaa taagctttgg     180

tgcaagagca gccaggtccc tcagtcaagg aacatctgtg acatctcctg tgacaaggtc     240

ctggatgatg acattactga tgacataatg tgtgccaaga agatcctgga tattaaagga     300

attgactact ggttggccca taaagccctc tgcactgaga agctggaaca gtggctttgt     360

gagaagttgt ga                                                         372


<210> 48
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> ALAB-Y1V3

<400> 48
aagcaatata caaaatgtga gctgtcccag ctgctgaaag acatagatgg ttatggaggc      60

atcgctttgc ctgaattgat ctgtaccatg gttcacacca gtggttatga cacacaagcc     120

atagttgaaa acaatgaaag cacggaatat ggactcgtcc agatcagtaa taagctttgg     180

tgcaagagca gccaggtccc tcagtcaagg aacatctgtg acatctcctg tgacaaggtc     240

ctggatgatg acattactga tgacataatg tgtgccaaga agatcctgga tattaaagga     300

attgactact ggttggccca taaagccctc tgcactgaga agctggaaca gtggctttgt     360

gagaagttgt ga                                                         372


<210> 49
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> ALAB-H1V3

<400> 49
aagcaacata caaaatgtga gctgtcccag ctgctgaaag acatagatgg ttatggaggc      60

atcgctttgc ctgaattgat ctgtaccatg gttcacacca gtggttatga cacacaagcc     120

atagttgaaa acaatgaaag cacggaatat ggactcgtcc agatcagtaa taagctttgg     180

tgcaagagca gccaggtccc tcagtcaagg aacatctgtg acatctcctg tgacaaggtc     240

ctggatgatg acattactga tgacataatg tgtgccaaga agatcctgga tattaaagga     300

attgactact ggttggccca taaagccctc tgcactgaga agctggaaca gtggctttgt     360

gagaagttgt ga                                                         372


<210> 50
<211> 263
<212> DNA
<213> Artificial Sequence

<220> 
<223> P43 promoter from genome of B. subtilis

<400> 50
agccattgaa catacggttg atttaataac tgacaaacat caccctcttg ctaaagcggc     60

caaggacgct gccgccgggg ctgtttgcgt ttttgccgtg atttcgtgta tcattggttt    120

acttattttt ttgccaaagc tgtaatggct gaaaattctt acatttattt tacattttta    180

gaaatgggcg tgaaaaaaag cgcgcgatta tgtaaaatat aaagtgatag cggtaccatt    240

ataggtaaga gaggaatgta cac                                            263


<210> 51
<211> 434
<212> DNA
<213> Artificial Sequence

<220> 
<223> PaprE promoter from genome of B. subtilis

<400> 51
cttatttctt cctccctctc aataattttt tcattctatc ccttttctgt aaagtttatt      60

tttcagaata cttttatcat catgctttga aaaaatatca cgataatatc cattgttctc     120

acggaagcac acgcaggtca tttgaacgaa ttttttcgac aggaatttgc cgggactcag     180

gagcatttaa cctaaaaaag catgacattt cagcataatg aacatttact catgtctatt     240

ttcgttcttt tctgtatgaa aatagttatt tcgagtctct acggaaatag cgagagatga     300

tatacctaaa tagagataaa atcatctcaa aaaaatgggt ctactaaaat attattccat     360

ctattacaat aaattcacag aatagtcttt taagtaagtc tactctgaat ttttttaaaa     420

ggagagggta aaga                                                       434


<210> 52
<211> 284
<212> DNA
<213> Artificial Sequence

<220> 
<223> PNprE promoter from genome of B. subtilis

<400> 52
tcctttactt cttattaagg cctcattcgg ttagacagcg gacttttcaa aaagtttcaa     60

gatgaaacaa aaatatctca tcttcccctt gatatgtaaa aaacataact cttgaatgaa    120

ccaccacatg acacttgact catcttgata ttattcaaca aaaacaaaca caggacaata    180

ctatcaattt tgtctagtta tgttagtttt tgttgagtat tccagaatgc tagtttaata    240

taacaatata aagttttcag tattttcaaa aagggggatt tatt                     284


<210> 53
<211> 148
<212> DNA
<213> Artificial Sequence

<220> 
<223> PamyE promoter from genome of B. subtilis

<400> 53
tagagtgatt gtgataattt taaatgtaag cgttaacaaa attctccagt cttcacatcg     60

gtttgaaagg aggaagcgga agaatgaagt aagagggatt tttgactccg aagtaagtct    120

tcaaaaaatc aaataaggag tgtcaaga                                       148


<210> 54
<211> 359
<212> DNA
<213> Artificial Sequence

<220> 
<223> PsipS promoter from genome of B. licheniformis

<400> 54
tgactgtctt tctttttccc gctcagggct gtatggatcg taattgtcat agtactcttc      60

gtagctgctc attttgaggc aatcaccgcc tttgggtcat gatatgataa tgtattataa     120

agaggggcgt tttgcctgtg catatataac ggtttttcgt gctgtaaata agctgatcaa     180

ctgacaaaat tcaacattga ataaaggatt tttcgtttca accgctaata ttatatgtat     240

caaccatttt tttacggctg cgcctgccgg tgcttgcgga aagcattcag acggacggcc     300

tgatatgata gaaggagcgg aatgccgatg aaacgatccg gaagtgaagg agagtactt      359


<210> 55
<211> 407
<212> DNA
<213> Artificial Sequence

<220> 
<223> PBlapr promoter from genome of B. licheniformis

<400> 55
tacgcctttc acatgagctg atttcatatc ttacacccgt ttctgtatgc gatatattgc      60

atattttaat agatgatcga ctaggccgca acctccttcg gcaaaaaatg atctcataaa     120

ataaatgaat agtattttca taaaatgaat cagacgaagc aatctcctgt cattcacgga     180

ccccgggacc tctttccctg ccaggttgaa gcggtctatt catactttcg aaccgaatat     240

ttttctaaaa cagttattaa taaccaataa atttaaattg gccgttcaaa aaaatgggtc     300

taccatataa ttcatttttt ttctataata aattaacaga ataattagaa tagagtatat     360

tattcttcta tttcaattat tctgaataaa acggaggaga gtgagta                   407


<210> 56
<211> 153
<212> DNA
<213> Artificial Sequence

<220> 
<223> PamyS promoter from genome of B. licheniformis

<400> 56
agtgaagaag cagagaggct attgaataaa tgagtagaaa gcgccatatc ggcgcttttc     60

ttttggaaga aaatataggg aaaatggtat ttgttaaaaa ttctgaatat ttatacaata    120

tcatatgttt cacattgaaa ggggaggaga atc                                 153


<210> 57
<211> 605
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pcry82 promoter, a synthetic promoter based on the pcry32a
      promoter from the B. thuringiensis

<400> 57
gtgctttttt tgttgacatt gaagaattat taatgttata attaattaaa gataatatct      60

ttgaattgta acgcccctca aaagtaagaa ctacaaaaaa agaatacgtt atatagaaat     120

atgtttgaac cttcttcaga ttacaaatat attcggacgg actctacctc aaatgcttat     180

ctaactatag aatgacatac aagcacaacc ttgaaaattt gaaaatataa ctaccaatga     240

acttgttcat gtgaattatc gctgtattta attttctcaa ttcaatatat aatatgccaa     300

tacattgtta caagtagaaa ttaagacacc cttgatagcc ttactatacc taacatgatg     360

tagtattaaa tgaatatgta aatatattta tgataagaag cgacttattt ataatcatta     420

catatttttc tattggaatg attaagattc caatagaata gtgtataaat tatttatctt     480

gaaaggaggg atgcctaaaa acgaagaaca ttaaaaacat atatttgcac cgtctaatgg     540

atttatgaaa aatcatttta tcagtttgaa aattatgtat tatgataaga aagggaggaa     600

gaaaa                                                                 605


<210> 58
<211> 630
<212> DNA
<213> Artificial Sequence

<220> 
<223> PAPase promoter from genome of B. licheniformis

<400> 58
gtaccttctg aggatttggc tctagaggac tggaaccgcg tcatatcaac caatttgacg      60

gggatgtttc taggatgccg ggaagcgatc ggctatatgc tcgaccataa tattaaaggc     120

tcggtcatca atatgtccag cgtccatcag caaatcccgt ggccgcattt tgtccattat     180

gcggcaagca agggcggggc aaaactgctg acggaaaccc ttgcccttga atatgccccg     240

aaaggaatcc gggtaaacgc gatcggcccc ggtgcaatcg atacgccgat taacgccgaa     300

aaattcgctg atcccgaatt gaaaaaaggc gttattgaat tgattcctat agggtatatc     360

ggaaagccgg aggaagtcgc agcctgcgcg gcctggctag catccgaaga agcaagctat     420

gtgacgggcc tgacgctgta tgtagacggc gggatgacaa aatacccggg attccaagcg     480

ggaaaagggt gaaaagaagg cggatgcctt cttttcatca taagcccatg aaaatttaca     540

taagattaat agaaagcaaa agtgctgttt acatagcaat gatagtgtag gtgtgtcaac     600

aaaatgcagt tatggaggga gtacataaga                                      630


<210> 59
<211> 675
<212> DNA
<213> Artificial Sequence

<220> 
<223> âPro1 gene fragments

<400> 59
aatatcaaaa ttgccgtcat tgacagcggg atctcccccc acgatgacct gtcgattgcc      60

ggcgggtatt cagctgtcag ttatacctct tcttacaaag atgataacgg ccacggaaca     120

catgtcgcag ggattatcgg agccaagcat aacggctacg gaattgacgg catcgcaccg     180

gaagcacaaa tatacgcggt taaagcgctt gatcagaacg gctcggggga tcttcaaagt     240

cttctccaag gaattgactg gtcgatcgca aacaggatgg acatcgtcaa tatgagcctt     300

ggcacgacgt cagacagcaa aatccttcat gacgccgtga acaaagcata tgaacaaggt     360

gttctgcttg ttgccgcaag cggtaacgac ggaaacggca agccagtgaa ttatccggcg     420

gcatacagca gtgtcgttgc ggtttcagca acaaacgaaa agaatcagct tgcctccttt     480

tcaacaactg gagatgaagt tgaattttca gcaccgggga caaacatcac aagcacttac     540

ttaaaccagt attatgcaac gggaagcgga acatcccaag cgacaccgca cgccgctgcc     600

atgtttgcct tgttaaaaca gcgtgatcct gccgagacaa acgtccagct tcgcgaggaa     660

atgcggaaaa actaa                                                      675


<210> 60
<211> 552
<212> DNA
<213> Artificial Sequence

<220> 
<223> âPro2 gene fragments

<400> 60
ggaaaaggca tcaaggtggc gattattgac actggggttg aatacaatca cccagatctg      60

aagaaaaact ttggacaata taaaggatac gattttgtgg acaatgatta cgatccaaaa     120

gaaacaccaa ccggcgatcc gaggggcgag gcaactgacc atggcacaca cgtagccgga     180

actgtggctg caaacggaac gattaaaggc gtagcgcctg atgccacact tcttgcttat     240

cgtgtgttag ggcctggcgg aagcggcaca acggaaaacg tcatcgcggg cgtggaacgt     300

gcagtgcagg acggggcaga tgtgatgaac ctgtctctcg gaaactcttt aaacaacccg     360

gactgggcga caagcacagc gcttgactgg gccatgtcag aaggcgttgt cgctgttacc     420

tcaaacggca acagcggacc gaacggctgg acagtcggat cgccgggcac atcaagagaa     480

gcgatttctg tcggtgcgac tcagctgccg ctcaatgaat acgccgtcac tttcggctcc     540

tactcttcat aa                                                         552


<210> 61
<211> 546
<212> DNA
<213> Artificial Sequence

<220> 
<223> âamyQ gene fragments

<400> 61
tggtcgttca atacgttaaa acacaatatg aaggatattc atgatgcagg atatacagcc      60

attcagacat ctccgattaa ccaagtaaag gaagggaatc aaggagataa aagcatgtcg     120

aactggtact ggctgtatca gccgacatcg tatcaaattg gcaaccgtta cttaggtact     180

gaacaagaat ttaaagaaat gtgtgcagcc gctgaagaat atggcataaa ggtcattgtt     240

gacgcggtca tcaatcatac caccagtgat tatgccgcga tttccaatga ggttaagagt     300

attccaaact ggacacatgg aaacacacaa attaaaaact ggtctgatcg atgggatgtc     360

acgcagaatt cattgctcgg gctgtatgac tggaatacac aaaatacaca agtacagtcc     420

tatctgaaac ggttcttaga cagggcattg aatgacgggg cagacggttt tcgatttgat     480

gccgccaaac atatagagct tccagatgat ggcagttacg gcagtcaatt ttggccgaat     540

atctaa                                                                546


<210> 62
<211> 567
<212> DNA
<213> Artificial Sequence

<220> 
<223> âyjeA gene

<400> 62
ctttgccatc aaggaggatt atatcgaact ttactttgac acatatcagg ttgcagcagg      60

ctatcttgga gagcaatcga ttgccattaa gaaaagtctt ttgaaagaca ttctgaaaga     120

acaatatatt gataaagcaa aaaataaaaa taaaatcaaa gaacaaaagc cgaagcatga     180

agtgatttct ttacccaaag aggaaacagt tgatccaaat caaaaagtca ttgcgcttac     240

tttcgacgac ggcccgaatc ctgcgacaac aaatcagatt ctagactcct tgaagaaata     300

taagggtcat gccacgtttt ttgttttggg gagcagagtt caatattatc cggaaacgct     360

aataagaatg ctgaaagaag gaaatgaggt tgggaaccat tcatggagcc atccgcttct     420

cacaaggctt tcagtgaaag aagcgttaaa gcagatcaat gatacgcaag acatcattga     480

aaaaataagc ggatatcgtc cgacgcttgt aagaccccca tacggcggca ttaatgatga     540

actgcggagt caaatgaaaa tggataa                                         567


<210> 63
<211> 546
<212> DNA
<213> Artificial Sequence

<220> 
<223> estA gene fragments without native signal peptide

<400> 63
gctgaacaca atccagtcgt tatggttcac ggtattggag gggcatcatt caattttgcg      60

ggaattaaga gctatctcgt atctcagggc tggtcgcggg acaagctgta tgcagttgat     120

ttttgggaca agacaggcac aaattataac aatggaccgg tattatcacg atttgtgcaa     180

aaggttttag atgaaacggg tgcgaaaaaa gtggatattg tcgctcacag catggggggc     240

gcgaacacac tttactacat aaaaaatctg gacggcggaa ataaagttgc aaacgtcgtg     300

acgcttggcg gcgcgaaccg tttgacgaca ggcaaggcgc ttccgggaac agatccaaat     360

caaaagattt tatacacatc catttacagc agtgccgata tgattgtcat gaattactta     420

tcaagattag atggtgctag aaacgttcaa atccatggcg ttggacacat cggccttctg     480

tacagcagcc aagtcaacag cctgattaaa gaagggctga acggcggggg ccagaatacg     540

aattaa                                                                546


<210> 64
<211> 549
<212> DNA
<213> Artificial Sequence

<220> 
<223> estB gene fragments without native signal peptide

<400> 64
gagtcagtac ataatcctgt cgttcttgtt catggaataa gtggtgcatc atacaacttt      60

ttcgctatta aaaactactt aatttctcaa ggctggcaaa gcaacaaact gtacgcaatt     120

gatttttatg ataaaacagg aaacaaccta aataacggcc cgcagcttgc ttcatatgtt     180

gaccgtgttt taaaagagac tggggcaaaa aaagtagata ttgtggctca tagtatggga     240

ggcgccaata cgctgtacta tattaaatat ttaggcgggg gcaataagat tcaaaatgtc     300

gtaacgcttg gaggggctaa tggtttagtg tcatcaaccg cgctgccggg cacagaccct     360

aatcaaaaga tcctctatac atctatttac agtctcaatg atcaaattgt catcaatagc     420

ttgtctcggt tacaaggagc gcgaaacatc cagctttatg gcatcggtca tattggcttg     480

ctttctaata gccaagtgaa cggctatatc aaagaagggc tgaatggcgg aggcctcaat     540

acaaattaa                                                             549


<210> 65
<211> 1146
<212> DNA
<213> Artificial Sequence

<220> 
<223> aprE gene

<400> 65
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg      60

gcgttcagca acatgtctgc gcaggctgcc ggaaaaagca gtacagaaaa gaaatacatt     120

gtcggattta aacagacaat gagtgccatg agttccgcca agaaaaagga tgttatttct     180

gaaaaaggcg gaaaggttca aaagcaattt aagtatgtta acgcggccgc agcaacattg     240

gatgaaaaag ctgtaaaaga attgaaaaaa gatccgagcg ttgcatatgt ggaagaagat     300

catattgcac atgaatatgc gcaatctgtt ccttatggca tttctcaaat taaagcgccg     360

gctcttcact ctcaaggcta cacaggctct aacgtaaaag tagctgttat cgacagcgga     420

attgactctt ctcatcctga cttaaacgtc agaggcggag caagcttcgt accttctgaa     480

acaaacccat accaggacgg cagttctcac ggtacgcatg tagccggtac gattgccgct     540

cttaataact caatcggtgt tctgggcgta gcgccaagcg catcattata tgcagtaaaa     600

gtgcttgatt caacaggaag cggccaatat agctggatta ttaacggcat tgagtgggcc     660

atttccaaca atatggatgt tatcaacatg agccttggcg gacctactgg ttctacagcg     720

ctgaaaacag tcgttgacaa agccgtttcc agcggtatcg tcgttgctgc cgcagccgga     780

aacgaaggtt catccggaag cacaagcaca gtcggctacc ctgcaaaata tccttctact     840

attgcagtag gtgcggtaaa cagcagcaac caaagagctt cattctccag cgcaggttct     900

gagcttgatg tgatggctcc tggcgtgtcc atccaaagca cacttcctgg aggcacttac     960

ggcgcttata acggaacgtc catggcgact cctcacgttg ccggagcagc agcgttaatt    1020

ctttctaagc acccgacttg gacaaacgcg caagtccgtg atcgtttaga aagcactgca    1080

acatatcttg gaaactcttt ctactatgga aaagggttaa tcaacgtaca agcagctgca    1140

caataa                                                               1146


<210> 66
<211> 1980
<212> DNA
<213> Artificial Sequence

<220> 
<223> amyE gene

<400> 66
atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg      60

tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat     120

gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc     180

aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca     240

tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac     300

tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa     360

tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc     420

atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac     480

tggacacatg gaaacacaca aattaaaaac tggtctgatc gatgggatgt cacgcagaat     540

tcattgctcg ggctgtatga ctggaataca caaaatacac aagtacagtc ctatctgaaa     600

cggttcttag acagggcatt gaatgacggg gcagacggtt ttcgatttga tgccgccaaa     660

catatagagc ttccagatga tggcagttac ggcagtcaat tttggccgaa tatcacaaat     720

acatctgcag agttccaata cggagaaatc ctgcaggata gtgcctccag agatgctgca     780

tatgcgaatt atatggatgt gacagcgtct aactatgggc attccataag gtccgcttta     840

aagaatcgta atctgggcgt gtcgaatatc tcccactatg catctgatgt gtctgcggac     900

aagctagtga catgggtaga gtcgcatgat acgtatgcca atgatgatga agagtcgaca     960

tggatgagcg atgatgatat ccgtttaggc tgggcggtga tagcttctcg ttcaggcagt    1020

acgcctcttt tcttttccag acctgaggga ggcggaaatg gtgtgaggtt cccggggaaa    1080

agccaaatag gcgatcgcgg gagtgcttta tttgaagatc aggctatcac tgcggtcaat    1140

agatttcaca atgtgatggc tggacagcct gaggaactct cgaacccgaa tggaaacaac    1200

cagatattta tgaatcagcg cggctcacat ggcgttgtgc tggcaaatgc aggttcatcc    1260

tctgtctcta tcaatacggc aacaaaattg cctgatggca ggtatgacaa taaagctgga    1320

gcgggttcat ttcaagtgaa cgatggtaaa ctgacaggca cgatcaatgc caggtctgta    1380

gctgtgcttt atcctgatga tattgcaaaa gcgcctcatg ttttccttga gaattacaaa    1440

acaggtgtaa cacattcttt caatgatcaa ctgacgatta ccttgcgtgc agatgcgaat    1500

acaacaaaag ccgtttatca aatcaataat ggaccagaga cggcgtttaa ggatggagat    1560

caattcacaa tcggaaaagg agatccattt ggcaaaacat acaccatcat gttaaaagga    1620

acgaacagtg atggtgtaac gaggaccgag aaatacagtt ttgttaaaag agatccagcg    1680

tcggccaaaa ccatcggcta tcaaaatccg aatcattgga gccaggtaaa tgcttatatc    1740

tataaacatg atgggagccg agtaattgaa ttgaccggat cttggcctgg aaaaccaatg    1800

actaaaaatg cagacggaat ttacacgctg acgctgcctg cggacacgga tacaaccaac    1860

gcaaaagtga tttttaataa tggcagcgcc caagtgcccg gtcagaatca gcctggcttt    1920

gattacgtgc taaatggttt atataatgac tcgggcttaa gcggttctct tccccattga    1980


<210> 67
<211> 1539
<212> DNA
<213> Artificial Sequence

<220> 
<223> amyL gene

<400> 67
atgaaacaac aaaaacggct ttacgcccga ttgctgccgc tgttatttgc gctcatcttc      60

ttgctgcctc attctgcagc agcggcggca aatcttaaag ggacgctgat gcagtatttt     120

gaatggtaca tgcccaatga cggccaacat tggaagcgct tgcaaaacga ctcggcatat     180

ttggctgaac acggtattac tgccgtctgg attcccccgg catataaggg aacgagccaa     240

gcggatgtgg gctacggtgc ttacgacctt tatgatttag gggagtttca tcaaaaaggg     300

acggttcgga caaagtacgg cacaaaagga gagctgcaat ctgcgatcaa aagtcttcat     360

tcccgcgaca ttaacgttta cggggatgtg gtcatcaacc acaaaggcgg cgctgatgcg     420

accgaagatg taaccgcggt tgaagtcgat cccgctgacc gcaaccgcgt aatttcagga     480

gaacaccgaa ttaaagcctg gacacatttt cattttccgg ggcgcggcag cacatacagc     540

gattttaaat ggcattggta ccattttgac ggaaccgatt gggacgagtc ccgaaagctg     600

aaccgcatct ataagtttca aggaaaggct tgggattggg aagtttccaa tgaaaacggc     660

aactatgatt atttgatgta tgccgacatc gattatgacc atcctgatgt cgcagcagaa     720

attaagagat ggggcacttg gtatgccaat gaactgcaat tggacggttt ccgtcttgat     780

gctgtcaaac acattaaatt ttcttttttg cgggattggg ttaatcatgt cagggaaaaa     840

acggggaagg aaatgtttac ggtagctgaa tattggcaga atgacttggg cgcgctggaa     900

aactatttga acaaaacaaa ttttaatcat tcagtgtttg acgtgccgct tcattatcag     960

ttccatgctg catcgacaca gggaggcggc tatgatatga ggaaattgct gaacggtacg    1020

gtcgtttcca agcatccgtt gaaagcggtt acatttgtcg ataaccatga tacacagccg    1080

gggcaatcgc ttgagtcgac tgtccaaaca tggtttaagc cgcttgctta cgcttttatt    1140

ctcacaaggg aatctggata ccctcaggtt ttctacgggg atatgtacgg gacgaaagga    1200

gactcccagc gcgaaattcc tgccttgaaa cacaaaattg aaccgatctt aaaagcgaga    1260

aaacagtatg cgtacggagc acagcatgat tatttcgacc accatgacat tgtcggctgg    1320

acaagggaag gcgacagctc ggttgcaaat tcaggtttgg cggcattaat aacagacgga    1380

cccggtgggg caaagcgaat gtatgtcggc cggcaaaacg ccggtgagac atggcatgac    1440

attaccggaa accgttcgga gccggttgtc atcaattcgg aaggctgggg agagtttcac    1500

gtaaacggcg ggtcggtttc aatttatgtt caaagatag                           1539


<210> 68
<211> 1566
<212> DNA
<213> Artificial Sequence

<220> 
<223> NprE gene

<400> 68
gtgggtttag gtaagaaatt gtctgttgct gtcgctgctt cgtttatgag tttatcaatc      60

agcctgccag gtgttcaggc tgctgaaggt catcagctta aagagaatca aacaaatttc     120

ctctccaaaa acgcgattgc gcaatcagaa ctctctgcac caaatgacaa ggctgtcaag     180

cagtttttga aaaagaacag caacattttt aaaggtgacc cttccaaaag gctgaagctt     240

gttgaaagca cgactgatgc ccttggatac aagcactttc gatatgcgcc tgtcgttaac     300

ggagtgccaa ttaaagattc gcaagtgatc gttcacgtcg ataaatccga taatgtctat     360

gcggtcaatg gtgaattaca caatcaatct gctgcaaaaa cagataacag ccaaaaagtc     420

tcttctgaaa aagcgctggc actcgctttc aaagctatcg gcaaatcacc agacgctgtt     480

tctaacggag cggccaaaaa cagcaataaa gccgaattaa aagcgataga aacaaaagac     540

ggcagctatc gtcttgctta cgacgtgacg attcgctatg tcgagcctga acctgcaaac     600

tgggaagtct tagttgacgc cgaaacaggc agcattttaa aacagcaaaa taaagtagaa     660

catgccgccg ccactggaag cggaacaacg ctaaagggcg caactgttcc tttgaacatc     720

tcttatgaag gcggaaaata tgttctaaga gatctttcaa aaccaacagg cacccaaatc     780

atcacatatg atttgcaaaa cagacaaagc cgccttccgg gcacgcttgt ctcaagcaca     840

acgaaaacat ttacatcttc atcacagcgg gcagccgttg acgcacacta taacctcggt     900

aaagtgtacg attattttta ttcaaacttt aaacgaaaca gctatgataa caaaggcagt     960

aaaatcgttt cttccgttca ctacggcact caatacaata acgctgcatg gacaggagac    1020

cagatgattt acggtgatgg cgacggttca ttcttctctc cgctttccgg ctcattagat    1080

gtgacagcgc atgaaatgac acatggcgtc acccaagaaa cagccaactt gatttatgaa    1140

aatcagccag gtgcattaaa cgagtctttc tctgacgtat tcgggtattt taacgataca    1200

gaagactggg acatcggtga agacattacg gtcagccagc ctgctcttcg cagcctgtcc    1260

aaccctacaa aatacaacca gcctgacaat tacgccaatt accgaaacct tccaaacaca    1320

gatgaaggcg attatggcgg tgtacacaca aacagcggaa ttccaaacaa agccgcttac    1380

aacaccatca caaaacttgg tgtatctaaa tcacagcaaa tctattaccg tgcgttaaca    1440

acgtacctca cgccttcttc cacgttcaaa gatgccaagg cagctctcat tcagtctgcc    1500

cgtgacctct acggctcaac tgatgccgct aaagttgaag cagcctggaa tgctgttgga    1560

ttgtaa                                                               1566


<210> 69
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> ALAB gene without native signal peptide

<400> 69
aagcaattca caaaatgtga gctgtcccag ctgctgaaag acatagatgg ttatggaggc      60

atcgctttgc ctgaattgat ctgtaccatg tttcacacca gtggttatga cacacaagcc     120

atagttgaaa acaatgaaag cacggaatat ggactcttcc agatcagtaa taagctttgg     180

tgcaagagca gccaggtccc tcagtcaagg aacatctgtg acatctcctg tgacaagttc     240

ctggatgatg acattactga tgacataatg tgtgccaaga agatcctgga tattaaagga     300

attgactact ggttggccca taaagccctc tgcactgaga agctggaaca gtggctttgt     360

gagaagttgt ga                                                         372


<210> 70
<211> 1140
<212> DNA
<213> Artificial Sequence

<220> 
<223> Blapr gene

<400> 70
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttaatgct cgtgttcacg      60

atggccttca gcgattccgc gtctgctgct cagccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcggg agtgaaaacc gcatccgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttgaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcac     300

gtagctcatg ctttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctacaa gggagcgaac gtaaaagtcg ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gcttcgtagc tggcgaagct     480

tataacaccg acggcaacgg acacggcacg catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgccg aacgtatcct tgtacgcggt taaagtgctg     600

aattcaagcg gaagcggatc ttacagcggc attgtaagcg gaatcgagtg ggcgacgaca     660

aacggcatgg atgttatcaa catgagcctt ggaggaccat caggctcaac agcgatgaaa     720

caggcggttg acaatgcata tgcaagaggg gttgtcgttg tggcggctgc tgggaacagc     780

ggatcttcag gaaacacgaa tacaatcggc tatcctgcga aatacgactc tgtcatcgca     840

gttggcgcgg tagactctaa cagcaacaga gcttcatttt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtgtac agcacttacc caaccagcac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagtac ggcgacttat    1080

ttgggaagct ccttctacta tggaaaaggt ctgatcaatg tcgaagctgc cgctcaataa    1140


<210> 71
<211> 615
<212> DNA
<213> Artificial Sequence

<220> 
<223> Lip gene

<400> 71
atgcgtcgtc attcattttt atctattcta ttgatttgca tgctgtctgt tgtgtccgta      60

ttttcgttcc ggccttcagc agcttccgcc gcttcccaca atccggtcgt catggtccac     120

ggcatcggcg gagccgatta taacttcatc ggcattaaat cgtatttaca atctcaaggc     180

tggacaagca gtgagcttta cgccatcaac tttatcgata aaacgggaaa taatataaac     240

aatgctccga gattatccga atacatcaag cgtgtgctga atcaaacagg agcatcaaaa     300

gtcgatattg tcgcccacag catgggcggg gccaatacgc tctattatat taaaaatctg     360

gatggtgcgg ataaagtcgg acatgtcgtc acccttgggg gcgctaatcg tctcgttaca     420

aacacggcgc ctcagaatga caaaatctca tacacttcga tttacagcac aagcgactat     480

atcgtcttaa acagcctctc caaacttgat ggtgcaaaca atgtgcaaat ctcaggcgta     540

agccatgtcg gtcttttgtt cagcagcaaa gtaaatgcct tgattaaaga cgggctgacc     600

gccagcggga aataa                                                      615


<210> 72
<211> 720
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFP gene

<400> 72
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac     120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc     180

ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag     240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc     300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg     360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac     420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac     480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc     540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     660

ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa     720


<210> 73
<211> 1140
<212> DNA
<213> Bacillus licheniformis

<220> 
<223> 10266apr gene

<400> 73
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttcatgct cgtgttcacg      60

atggcattca gcgattccgc ttctgctgct caaccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcagg agtgaaaacc gcatctgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttaaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcat     300

gtggcccatg ccttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctttaa gggagcgaat gtaaaagtag ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gctttgtggc tggcgaagct     480

tataacaccg acggcaacgg acacggcaca catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgcca agcgtatcct tgtacgcggt taaagtactg     600

aattcaagcg gaagcggatc atacagcggc attgtaagcg gaatcgagtg ggcgacaaca     660

aacggcatgg atgttatcaa tatgagcctt gggggagcat caggctcgac agcgatgaaa     720

caggcagtcg acaatgcata tgcaagaggg gttgtcgttg tagctgcagc agggaacagc     780

ggatcttcag gaaacacgaa tacaattggc tatcctgcga aatacgattc tgtcatcgct     840

gttggtgcgg tagactctaa cagcaacaga gcttcatttt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtatac agcacttacc caacgaacac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagcac ggcgacttat    1080

ttgggaagct ccttctacta tgggaaaggt ctgatcaatg tcgaagctgc cgctcaataa    1140


<210> 74
<211> 274
<212> PRT
<213> Bacillus licheniformis

<220> 
<223> 10266apr

<400> 74
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Phe Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Ser Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Ala 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Phe Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Asn Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln 
        

<210> 75
<211> 1140
<212> DNA
<213> Bacillus licheniformis

<220> 
<223> 10266apr-W4

<400> 75
atgatgagga aaaagagttt ttggcttggg atgctgacgg ccttcatgct cgtgttcacg      60

atggcattca gcgattccgc ttctgctgct caaccggcga aaaatgttga aaaggattat     120

attgtcggat ttaagtcagg agtgaaaacc gcatctgtca aaaaggacat catcaaagag     180

agcggcggaa aagtggacaa gcagtttaga atcatcaacg cggcaaaagc gaagctagac     240

aaagaagcgc ttaaggaagt caaaaatgat ccggatgtcg cttatgtgga agaggatcat     300

gtggcccatg ccttggcgca aaccgttcct tacggcattc ctctcattaa agcggacaaa     360

gtgcaggctc aaggctggaa gggagcgaat gtaaaagtag ccgtcctgga tacaggaatc     420

caagcttctc atccggactt gaacgtagtc ggcggagcaa gctatgtggc tggcgaagct     480

tataacaccg acggcaacgg acacggcaca catgttgccg gtacagtagc tgcgcttgac     540

aatacaacgg gtgtattagg cgttgcgcca agcgtatcct tgtacgcggt taaagtactg     600

aattcaagcg gaagcggatc atacagcggc attgtaagcg gaatcgagtg ggcgacaaca     660

aacggcatgg atgttatcaa tatgagcctt gggggagcat caggctcgac agcgatgaaa     720

caggcagtcg acaatgcata tgcaagaggg gttgtcgttg tagctgcagc agggaacagc     780

ggatcttcag gaaacacgaa tacaattggc tatcctgcga aatacgattc tgtcatcgct     840

gttggtgcgg tagactctaa cagcaacaga gcttcatggt ccagcgtcgg agcagagctt     900

gaagtcatgg ctcctggcgc aggcgtatac agcacttacc caacgaacac ttatgcaaca     960

ttgaacggaa cgtcaatggc ttctcctcat gtagcgggag cagcagcttt gatcttgtca    1020

aaacatccga acctttcagc ttcacaagtc cgcaaccgtc tctccagcac ggcgacttat    1080

ttgggaagct cctggtacta tgggaaaggt ctgatcaatg tcgaagctgc cgctcaataa    1140


<210> 76
<211> 274
<212> PRT
<213> Bacillus licheniformis

<220> 
<223> 10266apr-W4

<400> 76
Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      
Gln Ala Gln Gly Trp Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              
Ser Tyr Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  
Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  
Leu Gly Val Ala Pro Ser Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      
Ser Ser Gly Ser Gly Ser Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         
Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Ala 
        115                 120                 125             
Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 
Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 
Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     
Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Trp Ser Ser Val Gly 
            180                 185                 190         
Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             
Pro Thr Asn Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 
His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 
Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     
Gly Ser Ser Trp Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         
Ala Gln 
        

<210> 77
<211> 1146
<212> DNA
<213> Bacillus subtilis

<220> 
<223> 10073aprE geme

<400> 77
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg      60

gcgttcagca acatgtctgc gcaggctgcc ggaaaaagca gtacagaaaa gaaatacatt     120

gtcggattta aacagacaat gagtgccatg agttccgcca agaaaaagga tgttatttct     180

gaaaaaggcg gaaaggttca aaagcaattt aagtatgtta acgcggccgc agcaacattg     240

gatgaaaaag ctgtaaaaga attgaaaaaa gatccgagcg ttgcatatgt ggaagaagat     300

catattgcac atgaatatgc gcaatctgtt ccttatggca tttctcaaat taaagcgccg     360

gctcttcact ctcaaggcta cacaggctct aacgtaaaag tagctgttat cgacagcgga     420

attgactctt ctcatcctga cttaaacgtc agaggcggag caagcttcgt accttctgaa     480

acaaacccat accaggacgg cagttctcac ggtacgcatg tagccggtac gattgccgct     540

cttaataact caatcggtgt tctgggcgta gcgccaagcg catcattata tgcagtaaaa     600

gtgcttgatt caacaggaag cggccaatat agctggatta ttaacggcat tgaatgggcc     660

atttccaaca atatggatgt tattaacatg agccttggcg gaccttctgg ttctacagcg     720

ctgaaaacag tcgttgataa agccgtttcc agcggtatcg tcgttgctgc cgctgccgga     780

aacgaaggtt cgtccggaag ctcaagcaca gtcggctacc ctgcaaaata tccttctact     840

attgcggtag gtgcggtaaa cagcagcaac caaagagctt cattctcaag cgcaggttct     900

gagcttgatg tgatggctcc tggcgtatcc atccaaagca cacttcctgg aggcacttac     960

ggtgcttaca acggcacgtc catggcgact cctcacgttg ccggagcagc agcgctaatt    1020

ctttctaagc acccgacttg gacaaacgcg caagtccgtg atcgtttaga aagcactgca    1080

acatatcttg gaaactcttt ctactatgga aaagggttaa tcaacgtaca agcagctgca    1140

caataa                                                               1146


<210> 78
<211> 275
<212> PRT
<213> Bacillus subtilis

<220> 
<223> 10073aprE gene

<400> 78
Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      
His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          
Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         
Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 
Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     
Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Ala 
            180                 185                 190         
Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             
Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 
Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     
Leu Gly Asn Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ala Gln 
        275 

<210> 79
<211> 1146
<212> DNA
<213> Bacillus subtilis

<220> 
<223> 10076aprE-W7 gene

<400> 79
gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg      60

gcgttcagca acatgtctgc gcaggctgcc ggaaaaagca gtacagaaaa gaaatacatt     120

gtcggattta aacagacaat gagtgccatg agttccgcca agaaaaagga tgttatttct     180

gaaaaaggcg gaaaggttca aaagcaattt aagtatgtta acgcggccgc agcaacattg     240

gatgaaaaag ctgtaaaaga attgaaaaaa gatccgagcg ttgcatatgt ggaagaagat     300

catattgcac atgaatatgc gcaatctgtt ccttatggca tttctcaaat taaagcgccg     360

gctcttcact ctcaaggcta cacaggctct aacgtaaaag tagctgttat cgacagcgga     420

attgactctt ctcatcctga cttaaacgtc agaggcggag caagctgggt accttctgaa     480

acaaacccat accaggacgg cagttctcac ggtacgcatg tagccggtac gattgccgct     540

cttaataact caatcggtgt tctgggcgta gcgccaagcg catcattata tgcagtaaaa     600

gtgcttgatt caacaggaag cggccaatat agctggatta ttaacggcat tgaatgggcc     660

atttccaaca atatggatgt tattaacatg agccttggcg gaccttctgg ttctacagcg     720

ctgaaaacag tcgttgataa agccgtttcc agcggtatcg tcgttgctgc cgctgccgga     780

aacgaaggtt cgtccggaag ctcaagcaca gtcggctacc ctgcaaaata tccttctact     840

attgcggtag gtgcggtaaa cagcagcaac caaagagctt catggtcaag cgcaggttct     900

gagcttgatg tgatggctcc tggcgtatcc atccaaagca cacttcctgg aggcacttac     960

ggtgcttaca acggcacgtc catggcgact cctcacgttg ccggagcagc agcgctaatt    1020

ctttctaagc acccgacttg gacaaacgcg caagtccgtg atcgtttaga aagcactgca    1080

acatatcttg gaaactcttt ctactatgga aaagggttaa tcaacgtaca agcagctgca    1140

caataa                                                               1146


<210> 80
<211> 275
<212> PRT
<213> Bacillus subtilis

<220> 
<223> 10073aprE-W7

<400> 80
Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      
His Ser Trp Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          
Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              
Ser Trp Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         
Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 
Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     
Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Trp Ser Ser Ala 
            180                 185                 190         
Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             
Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 
Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     
Leu Gly Asn Ser Trp Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ala Gln 
        275 

