                         SEQUENCE LISTING

<110>  THE UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
 
<120>  METHODS FOR THAXTOMIN PRODUCTION AND MODIFIED STREPTOMYCES WITH 
       INCREASED THAXTOMIN PRODUCTION

<130>  222109.2080

<150>  US 62/052,681
<151>  2014-09-19

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  1059
<212>  DNA
<213>  Streptomyces scabies

<400>  1
atggtgacag gccacggggc acggggccgg agcggtgggc ggccgacgtt ggaggaggtc       60

gccgcacggg ccggagtggg ccgggggacg gtgtcccggg tgatcaacgg ctcgccccgg      120

gtgagcgacg cgacccgcgc ggcggtcgag gcggccgtcg cggagctggg ttacgtcccg      180

aacacggcgg cccgcgcgct cgcggcgaac cgtaccgacg cgatcgcgat ggtcgtgccc      240

gaaccggaga cccgcttctt ctcggagccg tacttctccg acatcctcaa gggtgtcgga      300

gcgcaactgt ccgacaccga gatgcagctc ctgctgatct tcgcgggcaa cgaccgggag      360

cgccggcgcc tcgcccagta cctggccgcg caccgcgtcg acggtgtcct cctggtctcc      420

gtccacgcgg acgacccgct ccccgatctg ctgtcgcaac tggaaatccc ggccgtcatc      480

agcggccccc gctccgagca cgagacgctc ccctcggtcg actccgacaa ctacggcggc      540

ggccgctcgg cggtcgagca cctcatcgca cgggggcgcg cccggatcgc cacgatcacc      600

ggccggctgg acgtctacgg cgcccagcgg cgcatcgagg gctaccgcga cgccctggag      660

gacgcgggcc gcgaggtgga cgagcgcctg atcgcccccg gtgacttcac ggaggagggc      720

ggccgccgag cgatgcgcga actcctggcc cgctgccccg acctcgacgc ggtcttcgcc      780

gagtcggacg tcatggccgc cggcgcccgc caggtgctcc gcgaggaggg ccgccgcata      840

cccgacgacg tggcgctggt cggctacgac gactcggcga tcgcccgcca catggacccg      900

ccgctcacca gcgtccgcca gccgatagag gagatgggcc gcgcgatgat cgacctcctc      960

ctggacgaga tcgcggaccg ccgcccggcg gtgtcgaggg gcttggaacg acgccaggtg     1020

gtgctgccga cggagctggt ggggcgggat tcttcctga                            1059


<210>  2
<211>  352
<212>  PRT
<213>  Streptomyces scabies

<400>  2

Met Val Thr Gly His Gly Ala Arg Gly Arg Ser Gly Gly Arg Pro Thr 
1               5                   10                  15      


Leu Glu Glu Val Ala Ala Arg Ala Gly Val Gly Arg Gly Thr Val Ser 
            20                  25                  30          


Arg Val Ile Asn Gly Ser Pro Arg Val Ser Asp Ala Thr Arg Ala Ala 
        35                  40                  45              


Val Glu Ala Ala Val Ala Glu Leu Gly Tyr Val Pro Asn Thr Ala Ala 
    50                  55                  60                  


Arg Ala Leu Ala Ala Asn Arg Thr Asp Ala Ile Ala Met Val Val Pro 
65                  70                  75                  80  


Glu Pro Glu Thr Arg Phe Phe Ser Glu Pro Tyr Phe Ser Asp Ile Leu 
                85                  90                  95      


Lys Gly Val Gly Ala Gln Leu Ser Asp Thr Glu Met Gln Leu Leu Leu 
            100                 105                 110         


Ile Phe Ala Gly Asn Asp Arg Glu Arg Arg Arg Leu Ala Gln Tyr Leu 
        115                 120                 125             


Ala Ala His Arg Val Asp Gly Val Leu Leu Val Ser Val His Ala Asp 
    130                 135                 140                 


Asp Pro Leu Pro Asp Leu Leu Ser Gln Leu Glu Ile Pro Ala Val Ile 
145                 150                 155                 160 


Ser Gly Pro Arg Ser Glu His Glu Thr Leu Pro Ser Val Asp Ser Asp 
                165                 170                 175     


Asn Tyr Gly Gly Gly Arg Ser Ala Val Glu His Leu Ile Ala Arg Gly 
            180                 185                 190         


Arg Ala Arg Ile Ala Thr Ile Thr Gly Arg Leu Asp Val Tyr Gly Ala 
        195                 200                 205             


Gln Arg Arg Ile Glu Gly Tyr Arg Asp Ala Leu Glu Asp Ala Gly Arg 
    210                 215                 220                 


Glu Val Asp Glu Arg Leu Ile Ala Pro Gly Asp Phe Thr Glu Glu Gly 
225                 230                 235                 240 


Gly Arg Arg Ala Met Arg Glu Leu Leu Ala Arg Cys Pro Asp Leu Asp 
                245                 250                 255     


Ala Val Phe Ala Glu Ser Asp Val Met Ala Ala Gly Ala Arg Gln Val 
            260                 265                 270         


Leu Arg Glu Glu Gly Arg Arg Ile Pro Asp Asp Val Ala Leu Val Gly 
        275                 280                 285             


Tyr Asp Asp Ser Ala Ile Ala Arg His Met Asp Pro Pro Leu Thr Ser 
    290                 295                 300                 


Val Arg Gln Pro Ile Glu Glu Met Gly Arg Ala Met Ile Asp Leu Leu 
305                 310                 315                 320 


Leu Asp Glu Ile Ala Asp Arg Arg Pro Ala Val Ser Arg Gly Leu Glu 
                325                 330                 335     


Arg Arg Gln Val Val Leu Pro Thr Glu Leu Val Gly Arg Asp Ser Ser 
            340                 345                 350         


<210>  3
<211>  1443
<212>  DNA
<213>  Streptomyces scabies

<400>  3
atgcctgaac ccgtgaatcc ggccaccccg gtgacctttc ctcccgcctt cctctggggc       60

gcggccacct ccgcgtacca gatcgagggg gcggtgcggg aggacggccg tacgccctcc      120

atctgggaca ccttcagtca cacgccgggc aagaccgccg gcggcgagaa cggtgacatc      180

gctgtcgacc actaccaccg ctaccgcgac gacgtggcga tgatggcgga cctgggcctc      240

aacgcgtacc gcttctccgt ctcctggtcg cgggtgcagc cgacggggcg gggcccggcc      300

gtccagaagg ggctcgactt ctaccgacgg ctggtcgacg agctgctggc caagggcatc      360

aagcccgccg tcaccctcta ccactgggac ctcccgcagg agctggagga cgccggcggc      420

tggcccgagc gggacatcgt gcaccggttc gccgagtacg cgcggatcat gggcgaggcg      480

ctcggcgacc gcgtcgagca gtggatcacc ctcaacgagc cgtggtgcac cgcgttcctg      540

ggctacggct ccggggtgca cgcgccgggc cgtacggacc cggtggcgtc cctgcgcgcg      600

gcccaccatc tgaacgtggc gcacggcctc ggcgtctcgg cgctgcggtc ggcgatgccc      660

gcccgcaact cgatcgcggt gagcctcaac tcctcggtgg tgcggccgat caccagctcc      720

ccggaggacc gggccgcggc ccggaagatc gacgacctcg cgaacggcgt cttccacgga      780

ccgatgctgc acggggccta cccggagacc ctgttcgccg cgacctcgtc gctgacggac      840

tggtcgttcg tgcgggacgg tgacgtggcg acggcccatc agccgctgga cgctctgggg      900

ctgaactact acacgccggc gctggtcggc gcggcggacg ccggcctgga gggcccccgc      960

gcggacggcc acggggcgag cgagcactcg ccgtggccgg ccgcggacga cgtcctgttc     1020

caccagaccc cgggcgagcg tacggagatg ggctggacca tcgacccgac gggcctgcac     1080

gagctgatca tgcggtacgc gcgggaggct ccgggcctgc cgatgtacgt gacggagaac     1140

ggcgccgcgt acgacgacaa gatggacgcg gacggccgtg tccacgaccc cgagcgcatc     1200

gcctacctgc acggccacct gcgggcggtc cggcgcgcga tcgccgaggg ggcggacgtg     1260

cgcgggtact acctgtggtc cctgatggac aacttcgagt gggcgtacgg ctacggcaag     1320

cgcttcggcg cggtgtacgt cgactacgcg accctgaccc gcacaccgaa gtcgagcgcg     1380

cactggtacg ggcaggcggc gaagacgggc gccctcccgc cgctggcgcc ggcgccggcg     1440

tag                                                                   1443


<210>  4
<211>  480
<212>  PRT
<213>  Streptomyces scabies

<400>  4

Met Pro Glu Pro Val Asn Pro Ala Thr Pro Val Thr Phe Pro Pro Ala 
1               5                   10                  15      


Phe Leu Trp Gly Ala Ala Thr Ser Ala Tyr Gln Ile Glu Gly Ala Val 
            20                  25                  30          


Arg Glu Asp Gly Arg Thr Pro Ser Ile Trp Asp Thr Phe Ser His Thr 
        35                  40                  45              


Pro Gly Lys Thr Ala Gly Gly Glu Asn Gly Asp Ile Ala Val Asp His 
    50                  55                  60                  


Tyr His Arg Tyr Arg Asp Asp Val Ala Met Met Ala Asp Leu Gly Leu 
65                  70                  75                  80  


Asn Ala Tyr Arg Phe Ser Val Ser Trp Ser Arg Val Gln Pro Thr Gly 
                85                  90                  95      


Arg Gly Pro Ala Val Gln Lys Gly Leu Asp Phe Tyr Arg Arg Leu Val 
            100                 105                 110         


Asp Glu Leu Leu Ala Lys Gly Ile Lys Pro Ala Val Thr Leu Tyr His 
        115                 120                 125             


Trp Asp Leu Pro Gln Glu Leu Glu Asp Ala Gly Gly Trp Pro Glu Arg 
    130                 135                 140                 


Asp Ile Val His Arg Phe Ala Glu Tyr Ala Arg Ile Met Gly Glu Ala 
145                 150                 155                 160 


Leu Gly Asp Arg Val Glu Gln Trp Ile Thr Leu Asn Glu Pro Trp Cys 
                165                 170                 175     


Thr Ala Phe Leu Gly Tyr Gly Ser Gly Val His Ala Pro Gly Arg Thr 
            180                 185                 190         


Asp Pro Val Ala Ser Leu Arg Ala Ala His His Leu Asn Val Ala His 
        195                 200                 205             


Gly Leu Gly Val Ser Ala Leu Arg Ser Ala Met Pro Ala Arg Asn Ser 
    210                 215                 220                 


Ile Ala Val Ser Leu Asn Ser Ser Val Val Arg Pro Ile Thr Ser Ser 
225                 230                 235                 240 


Pro Glu Asp Arg Ala Ala Ala Arg Lys Ile Asp Asp Leu Ala Asn Gly 
                245                 250                 255     


Val Phe His Gly Pro Met Leu His Gly Ala Tyr Pro Glu Thr Leu Phe 
            260                 265                 270         


Ala Ala Thr Ser Ser Leu Thr Asp Trp Ser Phe Val Arg Asp Gly Asp 
        275                 280                 285             


Val Ala Thr Ala His Gln Pro Leu Asp Ala Leu Gly Leu Asn Tyr Tyr 
    290                 295                 300                 


Thr Pro Ala Leu Val Gly Ala Ala Asp Ala Gly Leu Glu Gly Pro Arg 
305                 310                 315                 320 


Ala Asp Gly His Gly Ala Ser Glu His Ser Pro Trp Pro Ala Ala Asp 
                325                 330                 335     


Asp Val Leu Phe His Gln Thr Pro Gly Glu Arg Thr Glu Met Gly Trp 
            340                 345                 350         


Thr Ile Asp Pro Thr Gly Leu His Glu Leu Ile Met Arg Tyr Ala Arg 
        355                 360                 365             


Glu Ala Pro Gly Leu Pro Met Tyr Val Thr Glu Asn Gly Ala Ala Tyr 
    370                 375                 380                 


Asp Asp Lys Met Asp Ala Asp Gly Arg Val His Asp Pro Glu Arg Ile 
385                 390                 395                 400 


Ala Tyr Leu His Gly His Leu Arg Ala Val Arg Arg Ala Ile Ala Glu 
                405                 410                 415     


Gly Ala Asp Val Arg Gly Tyr Tyr Leu Trp Ser Leu Met Asp Asn Phe 
            420                 425                 430         


Glu Trp Ala Tyr Gly Tyr Gly Lys Arg Phe Gly Ala Val Tyr Val Asp 
        435                 440                 445             


Tyr Ala Thr Leu Thr Arg Thr Pro Lys Ser Ser Ala His Trp Tyr Gly 
    450                 455                 460                 


Gln Ala Ala Lys Thr Gly Ala Leu Pro Pro Leu Ala Pro Ala Pro Ala 
465                 470                 475                 480 


<210>  5
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cbstxtR-A S. Scabies

<400>  5
cgggagcgct ccca                                                         14


<210>  6
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cbstxtB S. scabies

<400>  6
ggggagcgct ccca                                                         14


<210>  7
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cbscebR-E S. Scabies

<400>  7
tgggagcgct ccca                                                         14


<210>  8
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
gattccacgc cagcgcggta gtgacgggag acgaccatga ttccggggat ccgtcgacc        59


<210>  9
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
caagcgcttc gtcatccagg tcgatctggg tcgcactcat gtaggctgga gctgcttc         58


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
ctcccacgag tgatgtgttg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
ccgtgtcctt cttcatggtg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
gtctggcagt tccaggagtc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
aggtgttcca ccacaggaag                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
ggacatccag acgcagtaca                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
ctcggtgttg agcttctcct                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
tggtcgaggt catcaacaag                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
tggacctcga tgaccttctc                                                   20


<210>  18
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
gagcgactgt ccttcatgg                                                    19


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
cgtcgtccag taccacgag                                                    19


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
cggctacttc ccgatggat                                                    19


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
ctcgatgtca ctcctggtca                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
ggatgcgatc cacttctgat                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
cgcaccgata tgttgtgttc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
ctgggttacg tcccgaacac                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
ccttgaggat gtcggagaag                                                   20


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
aaatctagac cagcgtgatc ttggtcttg                                         29


<210>  27
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
aaatctagac cgtgtccttc ttcatggtg                                         29


<210>  28
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
cgcgccccgt aacccgtcgc gcctatcgtg cgccgggtga ttccggggat ccgtcgacc        59


<210>  29
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
ccacgcgggg atgttgtgtg tgccgcaccg gcccggtcat gtaggctgga gctgcttc         58


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
acttcttctg gcccttcgtg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
gcgggctcct acgactactg                                                   20


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
catatggtga caggccacgg ggc                                               23


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
gaattcggaa gaatcccgcc ccacc                                             25


<210>  34
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
tgtcaataag cgggagcgct cccacagcgc tctc                                   34


<210>  35
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
gagagcgctg tgggagcgct cccgcttatt gaca                                   34


<210>  36
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
ccaggtactg tgggagcgct cccacgagtg atgt                                   34


<210>  37
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
acatcactcg tgggagcgct cccacagtac ctgg                                   34


<210>  38
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
ctcccccagg gggagcgctc ccactgcgct gta                                    33


<210>  39
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
tacagcgcag tgggagcgct ccccctgggg gag                                    33


<210>  40
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
ggttcaggca tggaagcgct cccattggtg gtcg                                   34


<210>  41
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
cgaccaccaa tgggagcgct tccatgcctg aacc                                   34


<210>  42
<211>  1369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Apramycin resistance gene deletion cassette

<400>  42
attccgggga tccgtcgacc tgcagttcga agttcctatt ctctagaaag tataggaact       60

tcgaagttcc cgccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata      120

agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg      180

ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata      240

tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta      300

tgcagcggaa aatgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc      360

cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc      420

agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg      480

ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa      540

agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt      600

ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc      660

cctgcgtgct gcgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga      720

cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga      780

cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc      840

ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctc actcgcctgc      900

aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca      960

cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc     1020

gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga     1080

ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa     1140

ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac     1200

agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg     1260

cgaagaatgc gatgccgctc gccagtcgat tggctgagct cataagttcc tattccgaag     1320

ttcctattct ctagaaagta taggaacttc gaagcagctc cagcctaca                 1369


<210>  43
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence cbsbglC

<400>  43
tggaagcgct ccca                                                         14


