               SEQUENCE LISTING

<110> Global Bioenergies
      Scientist of Fortune S.A.

<120> Method for producing isobutene from 3-methylcrotonyl-CoA

<130> X2019 PCT S3

<150> EP14 19 9152.1
<151> 2014-12-19

<150> EP14 18 5188.1
<151> 2014-09-17

<160> 13

<170> BiSSAP 1.2

<210> 1
<211> 422
<212> PRT
<213> Jeotgalicoccus sp. ATCC 8456


<400> 1
Met Ala Thr Leu Lys Arg Asp Lys Gly Leu Asp Asn Thr Leu Lys Val 
1               5                   10                  15      
Leu Lys Gln Gly Tyr Leu Tyr Thr Thr Asn Gln Arg Asn Arg Leu Asn 
            20                  25                  30          
Thr Ser Val Phe Gln Thr Lys Ala Leu Gly Gly Lys Pro Phe Val Val 
        35                  40                  45              
Val Thr Gly Lys Glu Gly Ala Glu Met Phe Tyr Asn Asn Asp Val Val 
    50                  55                  60                  
Gln Arg Glu Gly Met Leu Pro Lys Arg Ile Val Asn Thr Leu Phe Gly 
65                  70                  75                  80  
Lys Gly Ala Ile His Thr Val Asp Gly Lys Lys His Val Asp Arg Lys 
                85                  90                  95      
Ala Leu Phe Met Ser Leu Met Thr Glu Gly Asn Leu Asn Tyr Val Arg 
            100                 105                 110         
Glu Leu Thr Arg Thr Leu Trp His Ala Asn Thr Gln Arg Met Glu Ser 
        115                 120                 125             
Met Asp Glu Val Asn Ile Tyr Arg Glu Ser Ile Val Leu Leu Thr Lys 
    130                 135                 140                 
Val Gly Thr Arg Trp Ala Gly Val Gln Ala Pro Pro Glu Asp Ile Glu 
145                 150                 155                 160 
Arg Ile Ala Thr Asp Met Asp Ile Met Ile Asp Ser Phe Arg Ala Leu 
                165                 170                 175     
Gly Gly Ala Phe Lys Gly Tyr Lys Ala Ser Lys Glu Ala Arg Arg Arg 
            180                 185                 190         
Val Glu Asp Trp Leu Glu Glu Gln Ile Ile Glu Thr Arg Lys Gly Asn 
        195                 200                 205             
Ile His Pro Pro Glu Gly Thr Ala Leu Tyr Glu Phe Ala His Trp Glu 
    210                 215                 220                 
Asp Tyr Leu Gly Asn Pro Met Asp Ser Arg Thr Cys Ala Ile Asp Leu 
225                 230                 235                 240 
Met Asn Thr Phe Arg Pro Leu Ile Ala Ile Asn Arg Phe Val Ser Phe 
                245                 250                 255     
Gly Leu His Ala Met Asn Glu Asn Pro Ile Thr Arg Glu Lys Ile Lys 
            260                 265                 270         
Ser Glu Pro Asp Tyr Ala Tyr Lys Phe Ala Gln Glu Val Arg Arg Tyr 
        275                 280                 285             
Tyr Pro Phe Val Pro Phe Leu Pro Gly Lys Ala Lys Val Asp Ile Asp 
    290                 295                 300                 
Phe Gln Gly Val Thr Ile Pro Ala Gly Val Gly Leu Ala Leu Asp Val 
305                 310                 315                 320 
Tyr Gly Thr Thr His Asp Glu Ser Leu Trp Asp Asp Pro Asn Glu Phe 
                325                 330                 335     
Arg Pro Glu Arg Phe Glu Thr Trp Asp Gly Ser Pro Phe Asp Leu Ile 
            340                 345                 350         
Pro Gln Gly Gly Gly Asp Tyr Trp Thr Asn His Arg Cys Ala Gly Glu 
        355                 360                 365             
Trp Ile Thr Val Ile Ile Met Glu Glu Thr Met Lys Tyr Phe Ala Glu 
    370                 375                 380                 
Lys Ile Thr Tyr Asp Val Pro Glu Gln Asp Leu Glu Val Asp Leu Asn 
385                 390                 395                 400 
Ser Ile Pro Gly Tyr Val Lys Ser Gly Phe Val Ile Lys Asn Val Arg 
                405                 410                 415     
Glu Val Val Asp Arg Thr 
            420         

<210> 2
<211> 441
<212> PRT
<213> Macrococcus caseolyticus (strain JCSC5402)


<400> 2
Met Arg Val Glu Phe Thr Ile Asn Tyr Ile Asn Val Glu Gly Ile Ser 
1               5                   10                  15      
Met Ser Lys Arg Val Pro Lys Asp Arg Gly Ile Asp Asn Ser Leu Lys 
            20                  25                  30          
Ile Met Lys Glu Gly Tyr Glu Tyr Val Pro Ala Arg Met Lys Lys Phe 
        35                  40                  45              
Asn Thr Asn Ile Phe Glu Thr Arg Val Leu Gly Gly Lys Thr Ala Val 
    50                  55                  60                  
Val Ile Ser Gly Lys Asp Ala Ala Glu Leu Phe Tyr Asp Asn Asp Lys 
65                  70                  75                  80  
Thr Glu Arg Lys Gly Thr Leu Pro Lys Arg Val Val Lys Thr Leu Phe 
                85                  90                  95      
Gly Lys Gly Ala Ile His Thr Thr Thr Gly Lys Lys His Ile Asp Arg 
            100                 105                 110         
Lys Ala Leu Phe Met Ser Leu Met Thr Asp Glu Asn Leu Ala Tyr Leu 
        115                 120                 125             
Arg Lys Leu Thr Arg Ile Tyr Trp Phe Gln Asn Ile Glu His Met Gln 
    130                 135                 140                 
Tyr Lys Gln Lys Val Asn Val Tyr Glu Glu Ala Thr Glu Leu Leu Thr 
145                 150                 155                 160 
Lys Val Gly Leu Arg Trp Ala Gly Ile Val Asp His Pro Glu Asn Ile 
                165                 170                 175     
Gln Lys Met Ala Asp Asp Met Asn Lys Met Ile Asp Ser Phe Ser Ala 
            180                 185                 190         
Ile Gly Ser Leu Tyr Gly Gly Tyr Arg Glu Ala Lys Lys Ala Arg Ala 
        195                 200                 205             
Arg Val Glu Gln Phe Leu Glu Asp Gln Ile Thr Ala Val Arg Lys Gly 
    210                 215                 220                 
Lys Ile His Pro Glu Lys Gly Thr Ala Leu Tyr Glu Phe Ser His Trp 
225                 230                 235                 240 
Glu Asp Met Asn Gly Lys Pro Met Asp Ala Arg Leu Cys Ala Val Asp 
                245                 250                 255     
Leu Met Asn Val Ile Arg Pro Leu Val Ala Ile Asn Lys Phe Val Ser 
            260                 265                 270         
Phe Gly Val Leu Ala Leu His Glu Phe Pro Gly Glu Arg Val Arg Val 
        275                 280                 285             
Ala Leu Asn Glu Gly Asp Tyr Ala Tyr Lys Phe Val Gln Glu Val Arg 
    290                 295                 300                 
Arg Tyr Tyr Pro Phe Val Pro Phe Leu Pro Gly Lys Ala Lys Glu Asn 
305                 310                 315                 320 
Ile Thr Phe Asp Gly Tyr Lys Ile Gln Lys Asp Thr Met Met Leu Leu 
                325                 330                 335     
Asp Ile Tyr Gly Thr Leu His Arg Asp Asp Leu Phe Ser Glu Pro Glu 
            340                 345                 350         
Arg Phe Asn Pro Tyr Arg Phe Asp Asn Trp Asp Gly Ser Pro Phe Asp 
        355                 360                 365             
Leu Ile Pro Gln Gly Gly Gly Asp Tyr Tyr Thr Asn His Arg Cys Ala 
    370                 375                 380                 
Gly Glu Trp Met Thr Ile Ile Ile Met Glu Glu Thr Met Lys Phe Phe 
385                 390                 395                 400 
Ala Asn Glu Ile Ser Tyr Asp Val Pro Pro Gln Asp Phe Thr Val Asp 
                405                 410                 415     
Thr Thr Lys Phe Pro Gly Lys Val Ala Ser Gly Met Asp Ile Glu Asn 
            420                 425                 430         
Ile Arg Val Asn Ile Asp Arg Thr Lys 
        435                 440     

<210> 3
<211> 345
<212> PRT
<213> Myxococcus xanthus (strain DK 1622)


<400> 3
Met Lys Ala Val Val Leu Arg Ser Phe Gly Glu Ala Gly Asn Leu Lys 
1               5                   10                  15      
Met Glu Thr Met Pro Met Pro Arg Pro Gly Arg Gly Glu Val Leu Leu 
            20                  25                  30          
Arg Val His Ala Cys Gly Val Cys Tyr His Asp Val Ile Asn Arg Arg 
        35                  40                  45              
Gly Asn Leu Pro Arg Thr Ser Val Pro Ala Ile Leu Gly His Glu Ala 
    50                  55                  60                  
Ala Gly Glu Val Ile Glu Val Gly Pro Asp Thr Pro Gly Trp Lys Thr 
65                  70                  75                  80  
Gly Asp Arg Ala Ala Thr Leu Gln Arg Met Ser Cys Gly Asp Cys Ala 
                85                  90                  95      
Leu Cys Arg Ser Gly Arg Asn Ser Leu Cys Lys Thr Asp Asn Arg Phe 
            100                 105                 110         
Phe Gly Glu Glu Leu Pro Gly Gly Tyr Ala Gln Phe Met Val Ala Pro 
        115                 120                 125             
Val Gly Gly Leu Gly Arg Val Pro Ala Ser Leu Pro Trp Asn Glu Ala 
    130                 135                 140                 
Ala Thr Val Cys Cys Thr Thr Gly Thr Ala Val His Thr Val Arg Thr 
145                 150                 155                 160 
Arg Gly Lys Val Arg Ala Gly Glu Thr Val Leu Ile Thr Gly Ala Ser 
                165                 170                 175     
Gly Gly Val Gly Leu Ser Ser Val Gln Leu Ala Arg Leu Asp Gly Ala 
            180                 185                 190         
Arg Val Ile Ala Val Thr Ser Ser Glu Ala Lys Val Gln Ala Leu Lys 
        195                 200                 205             
Glu Ala Gly Ala Asp Glu Val Ile Val Ser Arg Gly Leu Asp Phe Ala 
    210                 215                 220                 
Ser Asp Val Arg Lys Arg Thr Gln Gly Ala Gly Val Asp Val Ala Val 
225                 230                 235                 240 
Glu Ile Val Gly Ser Ala Thr Phe Asp Gln Thr Leu Lys Ser Met Ala 
                245                 250                 255     
Pro Gly Gly Arg Val Val Val Val Gly Asn Leu Glu Ser Gly Met Val 
            260                 265                 270         
Gln Leu Asn Pro Gly Leu Val Ile Val Lys Glu Leu Glu Ile Leu Gly 
        275                 280                 285             
Ala Tyr Ala Thr Thr Gln Ala Glu Leu Asp Glu Ala Leu Arg Leu Thr 
    290                 295                 300                 
Ala Thr Gly Gly Val Arg Gln Phe Val Thr Asp Ala Val Pro Leu Ala 
305                 310                 315                 320 
Glu Ala Ala Lys Ala His Phe Arg Leu Glu Asn Arg Glu Val Ala Gly 
                325                 330                 335     
Arg Leu Val Leu Val Pro Pro Glu Ala 
            340                 345 

<210> 4
<211> 527
<212> PRT
<213> Rhodotorula minuta


<400> 4
Met Gly Ile Val Gln Glu Ala Ala Ala Phe Val Gly Ser Leu Thr Ala 
1               5                   10                  15      
Val Gln Leu Ala Gly Ala Phe Ala Ala Leu Val Leu Ala Phe His Val 
            20                  25                  30          
Val Pro Tyr Ile Leu Asp Pro His Gly Leu Arg Lys Tyr Pro Ala Ala 
        35                  40                  45              
Gly Pro Phe Gly Ile Ala Ala Phe Thr Asp Ala Trp Val Val Trp Gln 
    50                  55                  60                  
Ala Arg Arg Gly Lys Arg Phe Glu Ala Leu Asp Lys Ala His Arg Lys 
65                  70                  75                  80  
Tyr Gly Lys Tyr Val Arg Ile Ser Pro Arg Gln Val Ser Ile Ala Asp 
                85                  90                  95      
Pro Lys Ala Leu Asp Thr Ile Tyr Gly His Gly Thr Gly Thr Met Lys 
            100                 105                 110         
Pro Ala Phe Tyr Asp Ala Phe Val Gly Leu Lys Asn Val Arg Gly Leu 
        115                 120                 125             
Phe Asn Thr Arg Asp Arg Ala Glu His Thr Arg Lys Arg Lys Ile Val 
    130                 135                 140                 
Ser Ala Thr Phe Ala Gln Arg Asn Val Leu Glu Phe Glu Pro Tyr Ile 
145                 150                 155                 160 
Ala Ser Val Met Arg Gln Leu Leu Asn Lys Trp Asp Ala Met Cys Asp 
                165                 170                 175     
Lys Ala Val Lys Asp Gly Lys Gly Trp Val Thr Leu Asp Thr Leu Thr 
            180                 185                 190         
Trp Leu Asn Phe Leu Ala Phe Asp Ile Ile Gly Asp Leu Ala Phe Gly 
        195                 200                 205             
Ala Pro Phe Gly Met Val Glu Arg Glu Ala Asp Ile Cys Glu Ile Glu 
    210                 215                 220                 
Gln Glu Asp Gly Ser Ile Lys His Leu Pro Ala Val Thr Ile Leu Asn 
225                 230                 235                 240 
Glu Arg Gly Glu Tyr Ser Asn Cys Leu Gly Val Met Pro Pro His Trp 
                245                 250                 255     
Arg Pro Phe Ser Lys Tyr Val Asp Pro Trp Phe Ser Arg Gly His Ala 
            260                 265                 270         
Ser Val Ile Asn Leu Ala Gly Met Ala Arg Ala Arg Val Asn Ala Arg 
        275                 280                 285             
Leu Lys Ser Gly Ala Gly Asp Arg Lys Asp Leu Leu Ala Arg Leu Gln 
    290                 295                 300                 
Glu Ala Arg Asp Glu Ser Gly Asn Pro Met Asp Ile Asp Glu Leu Thr 
305                 310                 315                 320 
Ala Glu Ala Leu Thr Gln Leu Ile Ala Gly Ser Asp Thr Thr Ser Asn 
                325                 330                 335     
Ser Ser Cys Ala Ile Ile Phe Tyr Leu Ala Arg Asn Gln Asp Ala Gln 
            340                 345                 350         
Lys Lys Leu Gln Ala Glu Leu Asp Lys Thr Phe Lys Asp Arg Gly Ile 
        355                 360                 365             
Ser Gly Val Met Glu Tyr Glu Asp Ile Lys Thr Leu Pro Tyr Leu Glu 
    370                 375                 380                 
Ala Cys Ile Asn Glu Ala Leu Arg Met His Ser Thr Ser Ser Met Gly 
385                 390                 395                 400 
Leu Pro Arg Val Met Pro Ala Gln Gly Ala Asp Phe Gln Gly Glu His 
                405                 410                 415     
Phe Lys Glu Gly Thr Glu Val Ser Val Pro Ala Tyr Thr Ile His His 
            420                 425                 430         
Leu Glu Ser Ile Trp Gly Asp Pro Phe Asn Tyr Arg Pro Glu Arg Trp 
        435                 440                 445             
Leu Gly Ser Asp Ala Lys Gln Leu Glu Lys Ser Phe Ile Pro Phe Ser 
    450                 455                 460                 
Ile Gly Pro Arg Ser Cys Val Gly Arg Asn Leu Ala Thr Met Glu Leu 
465                 470                 475                 480 
Leu Val Phe Met Ser Thr Leu Phe Tyr Arg Tyr Asp Phe Lys Leu Ala 
                485                 490                 495     
Asp Glu Asn Gln Thr Lys Leu Glu Thr Ser Glu Gly Phe Leu Arg Lys 
            500                 505                 510         
Pro Leu Glu Ser Trp Ile Lys Met Arg Arg Arg Ser Ile Asp Ala 
        515                 520                 525         

<210> 5
<211> 260
<212> PRT
<213> Pseudomonas aeruginosa (strain UCBPP-PA14)


<400> 5
Met Ser Glu Phe Phe Asp Arg Thr Gly Pro Leu Gln Glu Ala Gly Ser 
1               5                   10                  15      
Tyr Pro Gln Trp Ala Gln Gln Leu Ile Val Asp Cys Gln Ala Ser Lys 
            20                  25                  30          
Asp Arg Val Ser Gly His Glu Leu Tyr Arg Arg Met Arg Asp Ala Glu 
        35                  40                  45              
Leu Ser Pro Ala Leu Met Arg Leu Tyr Leu Ile Gly Gly Trp Pro Val 
    50                  55                  60                  
Val Glu Gln Phe Pro Leu Tyr Met Ser Gln Asn Leu Leu Lys Thr Arg 
65                  70                  75                  80  
Phe Ala Arg His Pro Gly Glu Asp Met Ala Arg Arg Trp Leu Met Arg 
                85                  90                  95      
Asn Ile Arg Val Glu Leu Asn His Ala Asp Tyr Trp Leu His Trp Ala 
            100                 105                 110         
Glu Ala His Gly Val Ser Leu Ala Glu Ile Gln Ala Gln Asp Val Pro 
        115                 120                 125             
Ala Glu Leu His Ala Leu Ser His Trp Cys Trp His Thr Cys Ala Ser 
    130                 135                 140                 
Asp Ser Leu Pro Val Ala Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly 
145                 150                 155                 160 
Val Thr Gly Glu Trp Ser Ala Leu Val Cys Ser Asn Gly Val Tyr Glu 
                165                 170                 175     
Asn Ala Phe Pro Lys Glu Gly Arg Lys Arg Ala Met Lys Trp Leu Lys 
            180                 185                 190         
Leu His Ala Gln Tyr Asp Asp Ala His Pro Trp Glu Ala Leu Glu Ile 
        195                 200                 205             
Ile Cys Thr Leu Ala Gly Thr Asn Pro Ser Ala Glu Leu Arg Arg Gln 
    210                 215                 220                 
Leu Arg Asp Ala Ile Cys Lys Ser Tyr Asp Tyr Met Tyr Leu Phe Leu 
225                 230                 235                 240 
Glu Arg Cys Met Gln Leu Glu Glu Ala Arg Ser Ala Arg Lys Leu Ala 
                245                 250                 255     
Val Gly Ala Glu 
            260 

<210> 6
<211> 261
<212> PRT
<213> Pseudomonas syringae pv. tomato (strain DC3000)


<400> 6
Met Ile Asp Thr Phe Glu Arg Thr Gly Pro Leu Met Glu Ala Ser Ser 
1               5                   10                  15      
Tyr Pro Ala Trp Ala Gln Gln Leu Ile Asn Asp Cys Ser Pro Ala Lys 
            20                  25                  30          
Ala Arg Val Val Glu His Glu Leu Tyr Gln Gln Met Arg Asp Ala Lys 
        35                  40                  45              
Leu Ser Pro Gln Ile Met Arg Gln Tyr Leu Ile Gly Gly Trp Pro Val 
    50                  55                  60                  
Val Glu Gln Phe Ala Val Tyr Met Ala Lys Asn Leu Thr Lys Thr Arg 
65                  70                  75                  80  
Phe Gly Arg His Pro Gly Glu Asp Met Ala Arg Arg Trp Leu Met Arg 
                85                  90                  95      
Asn Ile Arg Val Glu Leu Asn His Ala Asp Tyr Trp Val Asn Trp Cys 
            100                 105                 110         
Ala Ala His Asp Val Thr Leu Glu Asp Leu His Asp Gln Arg Val Ala 
        115                 120                 125             
Pro Glu Leu His Ala Leu Ser His Trp Cys Trp Gln Thr Ser Ser Ser 
    130                 135                 140                 
Asp Ser Leu Ala Val Ala Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly 
145                 150                 155                 160 
Ala Thr Gly Glu Trp Ser Ala Val Val Cys Ser Thr Gly Val Tyr Ala 
                165                 170                 175     
Glu Ala Phe Ala Glu Glu Thr Arg Lys Lys Ser Met Lys Trp Leu Lys 
            180                 185                 190         
Met His Ala Gln Tyr Asp Asp Ala His Pro Trp Glu Ala Leu Glu Ile 
        195                 200                 205             
Ile Cys Thr Leu Val Gly Asn Lys Pro Ser Leu Gln Leu Gln Ala Glu 
    210                 215                 220                 
Leu Arg Gln Ala Val Thr Lys Ser Tyr Asp Tyr Met Tyr Leu Phe Leu 
225                 230                 235                 240 
Glu Arg Cys Ile Gln Leu Asp Lys Val Lys Ser Pro Arg Gly Arg Val 
                245                 250                 255     
Ala Ala Leu Glu Met 
            260     

<210> 7
<211> 270
<212> PRT
<213> Pseudomonas putida (strain F1 / ATCC 700007)


<400> 7
Met Glu Ile Thr Arg Ile Lys Glu Leu Lys Val Ile Asp Ala Phe Val 
1               5                   10                  15      
Arg Ile Gly Pro Leu Met Asp Pro Ala Ser Tyr Pro Gln Trp Ala Gln 
            20                  25                  30          
Gln Leu Ile Glu Asp Cys Arg Glu Ser Lys Arg Arg Val Val Glu His 
        35                  40                  45              
Glu Phe Tyr Ala Arg Leu Arg Asp Gly Gln Leu Lys Gln Ser Thr Ile 
    50                  55                  60                  
Arg Gln Tyr Leu Ile Gly Gly Trp Pro Val Val Glu Gln Phe Ser Leu 
65                  70                  75                  80  
Tyr Met Ala His Asn Leu Thr Lys Thr Arg Tyr Gly Arg His Gln Gly 
                85                  90                  95      
Glu Asp Met Ala Arg Arg Trp Leu Met Arg Asn Ile Arg Val Glu Leu 
            100                 105                 110         
Asn His Ala Asp Tyr Trp Val Asn Trp Cys Gln Ala His Gly Val His 
        115                 120                 125             
Leu His Glu Leu Gln Ala Gln Glu Val Pro Pro Glu Leu Asn Gly Leu 
    130                 135                 140                 
Asn Asp Trp Cys Trp Arg Val Cys Ala Thr Glu Asn Leu Ala Ile Ser 
145                 150                 155                 160 
Met Ala Ala Thr Asn Tyr Ala Ile Glu Gly Ala Thr Gly Glu Trp Ser 
                165                 170                 175     
Ala Val Val Cys Ser Thr Asp Thr Tyr Ala Gln Gly Phe Pro Glu Glu 
            180                 185                 190         
Gly Arg Lys Arg Ala Met Lys Trp Leu Lys Met His Ala Gln Tyr Asp 
        195                 200                 205             
Asp Ala His Pro Trp Glu Ala Leu Glu Ile Ile Cys Thr Leu Ala Gly 
    210                 215                 220                 
Glu Asn Pro Thr Leu Gly Leu Arg Thr Glu Leu Arg Arg Ala Ile Cys 
225                 230                 235                 240 
Lys Ser Tyr Asp Cys Met Phe Leu Phe Leu Glu Arg Cys Met Gln Leu 
                245                 250                 255     
Glu Gly Arg Gln Gln Gly Arg Met Arg Pro Ala Leu Ala Ala 
            260                 265                 270 

<210> 8
<211> 299
<212> PRT
<213> Bacillus subtilis (strain 168)


<400> 8
Met Lys Leu Lys Asp Leu Ile Gly Lys Ala Ser Ile His Lys Asn Lys 
1               5                   10                  15      
Thr Ile Ala Val Ala His Ala Glu Asp Glu Glu Val Ile Arg Ala Val 
            20                  25                  30          
Lys Leu Ala Ala Glu His Leu Ser Ala Arg Phe Leu Leu Thr Gly Asp 
        35                  40                  45              
Ser Lys Lys Leu Asn Glu Leu Thr Ser Ser Met Gln Gly His Gln Val 
    50                  55                  60                  
Glu Ile Val His Ala Asn Thr Pro Glu Glu Ser Ala Lys Leu Ala Val 
65                  70                  75                  80  
Arg Ala Val His His Lys Thr Ala Asp Val Leu Met Lys Gly Asn Val 
                85                  90                  95      
Pro Thr Ser Val Leu Leu Lys Ala Val Leu Asn Arg Gln Glu Gly Leu 
            100                 105                 110         
Arg Ser Ala Ser Val Leu Ser His Val Ala Val Phe Asp Ile Pro Asp 
        115                 120                 125             
Phe Asp Arg Leu Met Phe Val Thr Asp Ser Ala Met Asn Ile Ala Pro 
    130                 135                 140                 
Ser Leu Glu Glu Leu Arg Gln Ile Leu Gln Asn Ala Val His Val Ala 
145                 150                 155                 160 
His Ala Val Gly Asn Asn Met Pro Lys Ala Ala Ala Leu Ala Ala Val 
                165                 170                 175     
Glu Thr Val Asn Pro Lys Met Glu Ala Thr Val Asn Ala Ala Ala Leu 
            180                 185                 190         
Ala Gln Met Tyr Lys Arg Gly Gln Ile Lys Gly Cys Ile Val Asp Gly 
        195                 200                 205             
Pro Leu Ala Leu Asp Asn Ala Val Ser Gln Ile Ala Ala Ala Gln Lys 
    210                 215                 220                 
Lys Ile Ser Gly Asp Val Ala Gly Asn Ala Asp Ile Leu Leu Val Pro 
225                 230                 235                 240 
Thr Ile Glu Ala Gly Asn Ile Leu Tyr Lys Ser Leu Ile Tyr Phe Ala 
                245                 250                 255     
Lys Ala Ser Val Ala Ala Val Ile Thr Gly Ala Lys Ala Pro Ile Ala 
            260                 265                 270         
Leu Thr Ser Arg Ala Asp Ser Ala Glu Asn Lys Leu Tyr Ser Ile Ala 
        275                 280                 285             
Leu Ala Ile Cys Ala Ser Glu Glu Tyr Thr His 
    290                 295                 

<210> 9
<211> 273
<212> PRT
<213> Enterococcus faecalis MTUP9


<400> 9
Met Ile Thr Val Ser Ile Ala Gly Gly Ser Gln Pro Glu Ile Leu Gln 
1               5                   10                  15      
Leu Val Lys Lys Ala Leu Lys Glu Ala Glu Gln Pro Leu Gln Phe Ile 
            20                  25                  30          
Val Phe Asp Thr Asn Glu Asn Leu Asp Thr Glu Asn Leu Trp Lys Tyr 
        35                  40                  45              
Val His Cys Ser Asp Glu Ala Thr Val Ala Gln Glu Ala Val Ser Leu 
    50                  55                  60                  
Val Ala Thr Gly Gln Ala Gln Ile Leu Leu Lys Gly Ile Ile Gln Thr 
65                  70                  75                  80  
His Thr Leu Leu Lys Glu Met Leu Lys Ser Glu His Gln Leu Lys Asn 
                85                  90                  95      
Lys Pro Ile Leu Ser His Val Ala Met Val Glu Leu Pro Ala Gly Lys 
            100                 105                 110         
Thr Phe Leu Leu Thr Asp Cys Ala Met Asn Ile Ala Pro Thr Gln Ala 
        115                 120                 125             
Thr Leu Ile Glu Ile Val Glu Asn Ala Lys Glu Val Ala Gln Lys Leu 
    130                 135                 140                 
Gly Leu His His Pro Lys Ile Ala Leu Leu Ser Ala Ala Glu Asn Phe 
145                 150                 155                 160 
Asn Pro Lys Met Pro Ser Ser Val Leu Ala Lys Glu Val Thr Ala His 
                165                 170                 175     
Phe Asn Gly Gln Gln Glu Ala Thr Val Phe Gly Pro Leu Ser Leu Asp 
            180                 185                 190         
Leu Ala Thr Ser Glu Glu Ala Val Ala His Lys Arg Tyr Ser Gly Pro 
        195                 200                 205             
Ile Met Gly Asp Ala Asp Ile Leu Val Val Pro Thr Ile Asp Val Gly 
    210                 215                 220                 
Asn Cys Leu Tyr Lys Ser Leu Thr Leu Phe Gly His Ala Lys Val Gly 
225                 230                 235                 240 
Gly Thr Ile Val Gly Thr Lys Val Pro Val Val Leu Thr Ser Arg Ser 
                245                 250                 255     
Asp Ser Thr Glu Ser Lys Phe His Ser Leu Arg Phe Ala Met Arg Gln 
            260                 265                 270         
Val 
    

<210> 10
<211> 384
<212> PRT
<213> Lactobacillus casei W56


<400> 10
Met Arg Asp Cys Thr Thr Glu Arg Arg Cys Leu Met Thr Met His Pro 
1               5                   10                  15      
Lys Arg Asp Val Val Ile Val Ile Asn Pro Gly Ser Thr Ser Ser Lys 
            20                  25                  30          
Ile Ala Leu Phe Lys Ala Gly Lys Met Val Ala Glu Arg Thr Leu Asn 
        35                  40                  45              
His Ser Leu Ala Glu Leu Ser Gln Phe Asp Ser Val Ile Ala Gln Lys 
    50                  55                  60                  
Asp Phe Arg Met Gln Ala Ile Gln Glu Phe Leu Ala Asp Gln Asp Phe 
65                  70                  75                  80  
Ser Ala Ser Glu Val Leu Ala Val Ala Gly Arg Gly Gly Leu Leu Lys 
                85                  90                  95      
Pro Ile Pro Gly Gly Thr Tyr Ala Val Asn Glu Ala Met Leu Asp Asp 
            100                 105                 110         
Leu Thr Ala Ala Lys Arg Asn Glu His Ala Ser Asn Leu Gly Ala Gly 
        115                 120                 125             
Leu Ala Gln Gln Val Ala Asp Gln Tyr Gly Val Lys Ala Tyr Val Val 
    130                 135                 140                 
Asp Pro Pro Val Val Asp Glu Leu Gln Pro Leu Ala Arg Ile Ser Gly 
145                 150                 155                 160 
Leu Lys Gly Ile Glu Arg His Ser Ala Ala His Val Leu Asn Gln Lys 
                165                 170                 175     
Ala Met Ala Arg Gln Val Leu Ala Thr Met Gly Lys Thr Tyr Ala Thr 
            180                 185                 190         
Ser Arg Val Ile Val Ala His Ile Gly Gly Gly Leu Ser Ile His Ala 
        195                 200                 205             
His Glu Asn Gly Arg Met Ile Asp Gly Asn Asn Gly Ile Asp Gly Glu 
    210                 215                 220                 
Gly Pro Tyr Ser Pro Glu Arg Ala Gly Ser Leu Pro Leu Val Asp Phe 
225                 230                 235                 240 
Val Ala Lys Val Leu Ala Glu Arg Leu Thr Leu Asp Gln Val Lys Lys 
                245                 250                 255     
Leu Leu Ala Ser Gln Ser Gly Leu Arg Ser Tyr Leu Asn Asp Ile Ser 
            260                 265                 270         
Ile Lys Asn Ile Val Thr Arg Ile Ala Glu Gly Asp Glu Thr Ala Lys 
        275                 280                 285             
Phe Tyr Leu Asp Gly Met Ile Tyr Gln Ile Lys Lys Gln Ile Ala Glu 
    290                 295                 300                 
Met Ala Gly Val Leu Asn Gly Gln Val Asp Val Ile Ile Leu Thr Gly 
305                 310                 315                 320 
Gly Ala Ala Tyr Ala Thr Ala Val Thr Val Pro Leu Gln His Asp Leu 
                325                 330                 335     
Ala Trp Ile Ala Pro Val Val Val Arg Pro Gly Glu Met Glu Met Gln 
            340                 345                 350         
Ala Leu Tyr Glu Gly Val Met Arg Val Leu Asn His Glu Glu Pro Val 
        355                 360                 365             
Arg Val Tyr Gln Ser Asp Ala Ser Thr Ile Lys Gly Gly Thr Gly Arg 
    370                 375                 380                 



<210> 11
<211> 370
<212> PRT
<213> Geobacillus sp. GHH01


<400> 11
Met Glu Glu Gln Lys Phe Arg Ile Leu Thr Ile Asn Pro Gly Ser Thr 
1               5                   10                  15      
Ser Thr Lys Ile Gly Val Phe Glu Asn Glu Arg Pro Leu Leu Glu Lys 
            20                  25                  30          
Thr Ile Arg His Glu Ala Asp Val Leu Arg Gln Tyr Lys Thr Ile Ala 
        35                  40                  45              
Asp Gln Tyr Glu Phe Arg Lys Gln Thr Ile Leu Gln Ala Leu Asp Glu 
    50                  55                  60                  
Glu Gly Ile Asn Leu Ser Lys Leu Ser Ala Val Cys Gly Arg Gly Gly 
65                  70                  75                  80  
Leu Leu Arg Pro Ile Glu Gly Gly Thr Tyr Arg Val Asn Glu Ala Met 
                85                  90                  95      
Leu Glu Asp Leu Arg Arg Gly Tyr Ser Gly Gln His Ala Ser Asn Leu 
            100                 105                 110         
Gly Gly Ile Leu Ala His Glu Ile Ala Ser Ala Leu Asn Ile Pro Ala 
        115                 120                 125             
Phe Ile Val Asp Pro Val Val Val Asp Glu Leu Asp Pro Ile Ala Arg 
    130                 135                 140                 
Ile Ser Gly Phe Pro Leu Ile Glu Arg Arg Ser Ile Phe His Ala Leu 
145                 150                 155                 160 
Asn Gln Lys Ala Val Ala Arg Arg Val Ala Lys Gln Leu Gly Lys Arg 
                165                 170                 175     
Tyr Asp Glu Leu Asn Leu Ile Val Ala His Met Gly Gly Gly Ile Thr 
            180                 185                 190         
Val Gly Ala His Lys Gln Gly Arg Val Val Asp Val Asn Asn Gly Leu 
        195                 200                 205             
Asp Gly Glu Gly Pro Phe Ser Pro Glu Arg Ala Gly Thr Val Pro Ala 
    210                 215                 220                 
Gly Asp Leu Val Ala Leu Cys Phe Ser Gly Glu Tyr Tyr Arg Glu Glu 
225                 230                 235                 240 
Ile Met Asn Met Leu Val Gly Gly Gly Gly Leu Val Gly Tyr Leu Gly 
                245                 250                 255     
Thr Asn Asp Ala Val Lys Val Glu Asn Met Ile Glu Ala Gly Asp Glu 
            260                 265                 270         
Lys Ala Lys Leu Val Tyr Glu Ala Met Ala Tyr Gln Val Ala Lys Glu 
        275                 280                 285             
Ile Gly Ala Ala Ser Ala Val Leu Ser Gly Lys Val Asp Ala Ile Ile 
    290                 295                 300                 
Leu Thr Gly Gly Leu Ala Tyr Gly Lys Ser Phe Val Glu Gln Ile Thr 
305                 310                 315                 320 
Arg Arg Val Gln Trp Ile Ala Asp Val Ile Val His Pro Gly Glu Asn 
                325                 330                 335     
Glu Leu Gln Ala Leu Ala Glu Gly Ala Leu Arg Val Leu Arg Gly Glu 
            340                 345                 350         
Glu Glu Glu Lys Val Tyr Pro Gly Glu Ala Val Ser Pro Ile Pro Ala 
        355                 360                 365             
Arg Arg 
    370 

<210> 12
<211> 286
<212> PRT
<213> Escherichia coli (strain K12)


<400> 12
Met Ser Gln Ala Leu Lys Asn Leu Leu Thr Leu Leu Asn Leu Glu Lys 
1               5                   10                  15      
Ile Glu Glu Gly Leu Phe Arg Gly Gln Ser Glu Asp Leu Gly Leu Arg 
            20                  25                  30          
Gln Val Phe Gly Gly Gln Val Val Gly Gln Ala Leu Tyr Ala Ala Lys 
        35                  40                  45              
Glu Thr Val Pro Glu Glu Arg Leu Val His Ser Phe His Ser Tyr Phe 
    50                  55                  60                  
Leu Arg Pro Gly Asp Ser Lys Lys Pro Ile Ile Tyr Asp Val Glu Thr 
65                  70                  75                  80  
Leu Arg Asp Gly Asn Ser Phe Ser Ala Arg Arg Val Ala Ala Ile Gln 
                85                  90                  95      
Asn Gly Lys Pro Ile Phe Tyr Met Thr Ala Ser Phe Gln Ala Pro Glu 
            100                 105                 110         
Ala Gly Phe Glu His Gln Lys Thr Met Pro Ser Ala Pro Ala Pro Asp 
        115                 120                 125             
Gly Leu Pro Ser Glu Thr Gln Ile Ala Gln Ser Leu Ala His Leu Leu 
    130                 135                 140                 
Pro Pro Val Leu Lys Asp Lys Phe Ile Cys Asp Arg Pro Leu Glu Val 
145                 150                 155                 160 
Arg Pro Val Glu Phe His Asn Pro Leu Lys Gly His Val Ala Glu Pro 
                165                 170                 175     
His Arg Gln Val Trp Ile Arg Ala Asn Gly Ser Val Pro Asp Asp Leu 
            180                 185                 190         
Arg Val His Gln Tyr Leu Leu Gly Tyr Ala Ser Asp Leu Asn Phe Leu 
        195                 200                 205             
Pro Val Ala Leu Gln Pro His Gly Ile Gly Phe Leu Glu Pro Gly Ile 
    210                 215                 220                 
Gln Ile Ala Thr Ile Asp His Ser Met Trp Phe His Arg Pro Phe Asn 
225                 230                 235                 240 
Leu Asn Glu Trp Leu Leu Tyr Ser Val Glu Ser Thr Ser Ala Ser Ser 
                245                 250                 255     
Ala Arg Gly Phe Val Arg Gly Glu Phe Tyr Thr Gln Asp Gly Val Leu 
            260                 265                 270         
Val Ala Ser Thr Val Gln Glu Gly Val Met Arg Asn His Asn 
        275                 280                 285     

<210> 13
<211> 289
<212> PRT
<213> Pseudomonas putida


<400> 13
Met Ser His Val Leu Asp Asp Leu Val Asp Leu Leu Ser Leu Glu Ser 
1               5                   10                  15      
Ile Glu Glu Asn Leu Phe Arg Gly Arg Ser Gln Asp Leu Gly Phe Arg 
            20                  25                  30          
Gln Leu Tyr Gly Gly Gln Val Leu Gly Gln Ser Leu Ser Ala Ala Ser 
        35                  40                  45              
Gln Thr Val Glu Asp Ala Arg His Val His Ser Leu His Gly Tyr Phe 
    50                  55                  60                  
Leu Arg Pro Gly Asp Ala Ser Leu Pro Val Val Tyr Ser Val Asp Arg 
65                  70                  75                  80  
Val Arg Asp Gly Gly Ser Phe Ser Thr Arg Arg Val Thr Ala Ile Gln 
                85                  90                  95      
Lys Gly Gln Thr Ile Phe Thr Cys Ser Ala Ser Phe Gln Tyr Asp Glu 
            100                 105                 110         
Glu Gly Phe Glu His Gln Ala Gln Met Pro Asp Val Val Gly Pro Glu 
        115                 120                 125             
Asn Leu Pro Thr Glu Val Glu Leu Ala His Ala Met Ala Asp Gln Leu 
    130                 135                 140                 
Pro Glu Arg Ile Arg Asp Lys Val Leu Cys Ala Lys Pro Ile Glu Ile 
145                 150                 155                 160 
Arg Pro Val Thr Glu Arg Asp Pro Phe Asn Pro Lys Pro Gly Asp Pro 
                165                 170                 175     
Val Lys Tyr Ala Trp Phe Arg Ala Asp Gly Asn Leu Pro Asp Val Pro 
            180                 185                 190         
Ala Leu His Lys Tyr Met Leu Ala Tyr Ala Ser Asp Phe Gly Leu Leu 
        195                 200                 205             
Thr Thr Ala Leu Leu Pro His Gly Lys Ser Val Trp Gln Arg Asp Met 
    210                 215                 220                 
Gln Ile Ala Ser Leu Asp His Ser Leu Trp Phe His Gly Asn Leu Arg 
225                 230                 235                 240 
Ala Asp Gln Trp Leu Leu Tyr Ala Thr Asp Ser Pro Trp Ala Gly Asn 
                245                 250                 255     
Ser Arg Gly Phe Cys Arg Gly Ser Ile Phe Asn Gln Ala Gly Gln Leu 
            260                 265                 270         
Val Ala Ser Ser Ser Gln Glu Gly Leu Ile Arg His Arg Lys Asp Trp 
        275                 280                 285             
Ala 
    

