                         SEQUENCE LISTING

<110>  University of Nottingham
 
<120>  MODIFIED SPIDER SILK

<130>  JA74443P.WOP

<150>  GB1416089.9
<151>  2014-09-11

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified fusion protein sequence

<400>  1
atggcacatc atcaccacca ccacaaagaa accgcggctg caaaattcga acgtcaacac       60

atggacagct ccagcggtct ggttccgcag ggatctatga gcgataaaat tattcacctg      120

actgacgaca gttttgacac ggatgtactc aaagcggacg gggcgatcct cgtcgatttc      180

tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga ttctggatga aatcgctgac      240

gaatatcagg gcaaactgac cgttgcaaaa ctgaacatcg atcaaaaccc tggcactgcg      300

ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt tcaaaaacgg tgaagtggcg      360

gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag agttcctcga cgctaacctg      420

gccggtaccg atgatgatga taagcttgtt ccgcgtggat ccggtaactc gggtatccaa      480

ggccaaggtg gctacggtgg tctgggccag ggcggctatg gccaaggcgc tggcagctcc      540

gcggcagctg cagcggccgc agctgcggcc gccgccggtg gccagggtgg tcagggccaa      600

ggcggctacg gccaaggtag cggcggtagc gctgccgcag cagcagcggc ggcagccgcc      660

gcagccgcag cggcaggccg tggtcaaggt ggttacggtc agggtagcgg tggtaatgcg      720

gcagcggcgg cagcagccgc agcggcagcc gccgcggcgg cgggtcaggg tggtcagggc      780

ggttatggcc gccagtccca aggtgcgggt agcgcagcag cagcagctgc agcggctgct      840

gctgcagccg cggccggttc cggtcaaggc ggttacggcg gtcaaggcca gggtggctat      900

ggtcaaagca gcgcaagcgc gagcgcggcc gcgtctgcgg cgagcaccgt tgcgaacagc      960

gttagccgtc tgtccagccc gagcgccgtg agccgcgtta gctcggctgt cagcagcctg     1020

gtgagcaatg gccaggtcaa tatggctgcc ctgccgaaca tcattagcaa tatcagcagc     1080

tctgtgtctg cgagcgctcc gggtgcgagc ggttgcgagg tgatcgtaca ggcgctgctg     1140

gaagtcatca cggcgttggt gcagattgtc agctctagca gcgttggtta cattaaccca     1200

agcgcggtta atcagattac taacgttgtg gcgaacgcga tggcccaggt catgggttaa     1260


<210>  2
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified fusion protein sequence

<400>  2
atggcacatc atcaccacca ccacaaagaa accgcggctg caaaattcga acgtcaacac       60

atggacagct ccagcggtct ggttccgcag ggatctatga gcgataaaat tattcacctg      120

actgacgaca gttttgacac ggatgtactc aaagcggacg gggcgatcct cgtcgatttc      180

tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga ttctggatga aatcgctgac      240

gaatatcagg gcaaactgac cgttgcaaaa ctgaacatcg atcaaaaccc tggcactgcg      300

ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt tcaaaaacgg tgaagtggcg      360

gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag agttcctcga cgctaacctg      420

gccggtaccg atgatgatga taagcttgtt ccgcgtggat ccggtaactc gggtatccaa      480

ggccaaggtg gctacggtgg tctgggccag ggcggctatg gccaaggcgc tggcagctcc      540

gcggcagctg cagcggccgc agctgcggcc gccgccggtg gccagggtgg tcagggccaa      600

ggcggctacg gccaaggtag cggcggtagc gctgccgcag cagcagcggc ggcagccgcc      660

gcagccgcag cggcaggcaa aggtcaaggt ggttacggtc agggtagcgg tggtaatgcg      720

gcagcggcgg cagcagccgc agcggcagcc gccgcggcgg cgggtcaggg tggtcagggc      780

ggttatggcc gccagtccca aggtgcgggt agcgcagcag cagcagctgc agcggctgct      840

gctgcagccg cggccggttc cggtcaaggc ggttacggcg gtcaaggcca gggtggctat      900

ggtcaaagca gcgcaagcgc gagcgcggcc gcgtctgcgg cgagcaccgt tgcgaacagc      960

gttagccgtc tgtccagccc gagcgccgtg agccgcgtta gctcggctgt cagcagcctg     1020

gtgagcaatg gccaggtcaa tatggctgcc ctgccgaaca tcattagcaa tatcagcagc     1080

tctgtgtctg cgagcgctcc gggtgcgagc ggttgcgagg tgatcgtaca ggcgctgctg     1140

gaagtcatca cggcgttggt gcagattgtc agctctagca gcgttggtta cattaaccca     1200

agcgcggtta atcagattac taacgttgtg gcgaacgcga tggcccaggt catgggttaa     1260


<210>  3
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  3

Gly Ser Ala Met Gly Tyr Leu Trp Ile Gln Gly Gln Gly Gly Tyr Gly 
1               5                   10                  15      


Gly Leu Gly Gln Gly Gly Tyr Gly Gln Gly Ala Gly Ser Ser Ala Ala 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Gln Gly Gly Gln 
        35                  40                  45              


Gly Gln Gly Gly Tyr Gly Gln Gly Ser Gly Gly Ser Ala Ala Ala Ala 
    50                  55                  60                  


Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Arg Gly Gln Gly 
65                  70                  75                  80  


Gly Tyr Gly Gln Gly Ser Gly Gly Asn Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ala Ala Ala Ala Ala Ala Ala Ala Gly Gln Gly Gly Gln Gly Gly Tyr 
            100                 105                 110         


Gly Arg Gln Ser Gln Gly Ala Gly Ser Ala Ala Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ala Ala Ala Ala Gly Ser Gly Gln Gly Gly Tyr Gly Gly 
    130                 135                 140                 


Gln Gly Gln Gly Gly Tyr Gly Gln Ser Ser Ala Ser Ala Ser Ala Ala 
145                 150                 155                 160 


Ala Ser Ala Ala Ser Thr Val Ala Asn Ser Val Ser Arg Leu Ser Ser 
                165                 170                 175     


Pro Ser Ala Val Ser Arg Val Ser Ser Ala Val Ser Ser Leu Val Ser 
            180                 185                 190         


Asn Gly Gln Val Asn Met Ala Ala Leu Pro Asn Ile Ile Ser Asn Ile 
        195                 200                 205             


Ser Ser Ser Val Ser Ala Ser Ala Pro Gly Ala Ser Gly Cys Glu Val 
    210                 215                 220                 


Ile Val Gln Ala Leu Leu Glu Val Ile Thr Ala Leu Val Gln Ile Val 
225                 230                 235                 240 


Ser Ser Ser Ser Val Gly Tyr Ile Asn Pro Ser Ala Val Asn Gln Ile 
                245                 250                 255     


Thr Asn Val Val Ala Asn Ala Met Ala Gln Val Met Gly 
            260                 265                 


<210>  4
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  4

Gly Gly Ser Gly Asn Ser Gly Ile Gln Gly Gln Gly Gly Tyr Gly Gly 
1               5                   10                  15      


Leu Gly Gln Gly Gly Tyr Gly Gln Gly Ala Gly Ser Ser Ala Ala Ala 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Gln Gly Gly Gln Gly 
        35                  40                  45              


Gln Gly Gly Tyr Gly Gln Gly Ser Gly Gly Ser Ala Ala Ala Ala Ala 
    50                  55                  60                  


Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Arg Gly Gly Gly Tyr 
65                  70                  75                  80  


Gly Gln Gly Ser Gly Gly Asn Ala Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ala Ala Ala Ala Ala Ala Gly Gln Gly Gly Gln Gly Gly Tyr Gly Arg 
            100                 105                 110         


Gln Ser Gln Gly Ala Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ala Ala Gly Ser Gly Gln Gly Gly Tyr Gly Gly Gln Gly 
    130                 135                 140                 


Gln Gly Gly Tyr Gly Gln Ser Ser Ala Ser Ala Ser Ala Ala Ala Ser 
145                 150                 155                 160 


Ala Ala Ser Thr Val Ala Asn Ser Val Ser Arg Leu Ser Ser Pro Ser 
                165                 170                 175     


Ala Val Ser Arg Val Ser Ser Ala Val Ser Ser Leu Val Ser Asn Gly 
            180                 185                 190         


Gln Val Asn Met Ala Ala Leu Pro Asn Ile Ile Ser Asn Ile Ser Ser 
        195                 200                 205             


Ser Val Ser Ala Ser Ala Pro Gly Ala Ser Gly Cys Glu Val Ile Val 
    210                 215                 220                 


Gln Ala Leu Leu Glu Val Ile Thr Ala Leu Val Gln Ile Val Ser Ser 
225                 230                 235                 240 


Ser Ser Val Gly Tyr Ile Asn Pro Ser Ala Val Asn Gln Ile Thr Asn 
                245                 250                 255     


Val Val Ala Asn Ala Met Ala Gln Val Met Gly 
            260                 265         


<210>  5
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  MOD_RES
<222>  (196)..(196)
<223>  Xaa = L-Azidohomoalanine

<220>
<221>  MOD_RES
<222>  (262)..(262)
<223>  Xaa = L-Azidohomoalanine

<220>
<221>  MOD_RES
<222>  (266)..(266)
<223>  Xaa = L-Azidohomoalanine

<400>  5

Gly Ser Gly Asn Ser Gly Ile Gln Gly Gln Gly Gly Tyr Gly Gly Leu 
1               5                   10                  15      


Gly Gln Gly Gly Tyr Gly Gln Gly Ala Gly Ser Ser Ala Ala Ala Ala 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Gln Gly Gly Gln Gly Gln 
        35                  40                  45              


Gly Gly Tyr Gly Gln Gly Ser Gly Gly Ser Ala Ala Ala Ala Ala Ala 
    50                  55                  60                  


Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Lys Gly Gln Gly Gly Tyr 
65                  70                  75                  80  


Gly Gln Gly Ser Gly Gly Asn Ala Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ala Ala Ala Ala Ala Ala Gly Gln Gly Gly Gln Gly Gly Tyr Gly Arg 
            100                 105                 110         


Gln Ser Gln Gly Ala Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ala Ala Gly Ser Gly Gln Gly Gly Tyr Gly Gly Gln Gly 
    130                 135                 140                 


Gln Gly Gly Tyr Gly Gln Ser Ser Ala Ser Ala Ser Ala Ala Ala Ser 
145                 150                 155                 160 


Ala Ala Ser Thr Val Ala Asn Ser Val Ser Arg Leu Ser Ser Pro Ser 
                165                 170                 175     


Ala Val Ser Arg Val Ser Ser Ala Val Ser Ser Leu Val Ser Asn Gly 
            180                 185                 190         


Gln Val Asn Xaa Ala Ala Leu Pro Asn Ile Ile Ser Asn Ile Ser Ser 
        195                 200                 205             


Ser Val Ser Ala Ser Ala Pro Gly Ala Ser Gly Cys Glu Val Ile Val 
    210                 215                 220                 


Gln Ala Leu Leu Glu Val Ile Thr Ala Leu Val Gln Ile Val Ser Ser 
225                 230                 235                 240 


Ser Ser Val Gly Tyr Ile Asn Pro Ser Ala Val Asn Gln Ile Thr Asn 
                245                 250                 255     


Val Val Ala Asn Ala Xaa Ala Gln Val Xaa Gly 
            260                 265         


