                         SEQUENCE LISTING

<110>  Agency For Science Technology and Research
 
<120>  A Method to Up-Regulate Cancer Stem Cell Markers For The  
       Generation of Antigen-Specific Cytotoxic Effector T Cells

<130>  9869SG3314

<160>  205   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Oct4 expression (forward primer)

<400>  1
tattcagcca aacgaccatc                                                   20


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Oct4 expression (reverse primer)

<400>  2
gcctctcact cggttctc                                                     18


<210>  3
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Sox2 expression (forward primer)

<400>  3
acgacgtgag cgccctgcag tacaa                                             25


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Sox2 expression (reverse primer)

<400>  4
gctggagctg gcctcggact tgacc                                             25


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Klf4 expression (forward primer)

<400>  5
cctacacaaa gagttcccat c                                                 21


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total Klf4 expression reverse primer)

<400>  6
agtgcctggt cagttcatc                                                    19


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total c-Myc expression (forward primer)

<400>  7
aggaacaaga agatgaggaa g                                                 21


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify total c-Myc expression (reverse primer)

<400>  8
tgcgtagttg tgctgatg                                                     18


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Oct4 expression (forward primer)

<400>  9
gtactcctcg gtccctttcc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Oct4 expression (reverse primer)

<400>  10
cacctgcaag tttcagcaaa                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Sox2 expression (forward primer)

<400>  11
catgtcccag cactaccaga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Sox2 expression (reverse primer)

<400>  12
acatcccctg cttgtttcaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Klf4 expression (forward primer)

<400>  13
gaccacctcg ccttacacat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous Klf4 expression (reverse primer)

<400>  14
ccaaaagacg gcaatatggt                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous c-Myc expression (forward primer)

<400>  15
aagaggactt gttgcggaaa                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify exogenous c-Myc expression (reverse primer)

<400>  16
ggcattaaag cagcgtatcc                                                   20


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Oct4 expression (forward primer)

<400>  17
aaggaattgg gaacacaaag g                                                 21


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Oct4 expression (reverse primer)

<400>  18
caagagcatc attgaacttc ac                                                22


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Sox2 expression (fowrard primer)

<400>  19
gggaaatggg aggggtgcaa aagagg                                            26


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Sox2 expression (reverse primer)

<400>  20
ttgcgtgagt gtggatggga ttggtg                                            26


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Klf4 expression (forward primer)

<400>  21
tggtgcttgg tgagtcttg                                                    19


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous Klf4 expression (reverse primer)

<400>  22
aggtcataaa tgttgatcgg aag                                               23


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous c-Myc expression (forward primer)

<400>  23
ccttgccgca tccacgaaac                                                   20


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify endogenous c-Myc expression (reverse primer)

<400>  24
ccttgctcgg gtgttgtaag ttc                                               23


<210>  25
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Adaptor sequence bearing EcoRI-AscI-SbfI restriction sites

<400>  25
acagaattcc tgcgcgccaa gttacggcgc gccc                                   34


<210>  26
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Adaptor sequence bearing EcoRI-AscI-SbfI restriction sites

<400>  26
ttagcataca ttatacctgc aggcac                                            26


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of OKSM-eGFP donor at AAVS1 
       locus (forward primer)

<400>  27
gctacgtccc ttcggccctc aatc                                              24


<210>  28
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of OKSM-eGFP donor at AAVS1 
       locus (reverse primer)

<400>  28
gcctccctaa gacccagaag tccag                                             25


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe design for Southern blot to detect multiple integration of 
       OKSM-eGFP cassette in genome (forward primer)

<400>  29
gactggtatt cttaactatg ttgctcc                                           27


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe design for Southern blot to detect multiple integration of 
       OKSM-eGFP cassette in genome (reverse primer)

<400>  30
caaagggaga tccgactcgt ctga                                              24


<210>  31
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Oct3/4 promoter (forward primer)

<400>  31
gaggttggag tagaaggatt gttttggttt                                        30


<210>  32
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Oct3/4 promoter (reverse primer)

<400>  32
cccccctaac ccatcacctc caccacctaa                                        30


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Oct3/4 promoter (forward primer)

<400>  33
gaggctggag cagaaggatt gctttggccc                                        30


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Oct3/4 promoter (reverse primer)

<400>  34
cccccctggc ccatcacctc caccacctgg                                        30


<210>  35
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Nanog promoter (forward primer)

<400>  35
tggttaggtt ggttttaaat ttttg                                             25


<210>  36
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Nanog promoter (reverse primer)

<400>  36
aacccaccct tataaattct caatta                                            26


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Nanog promoter (forward primer)

<400>  37
tggccaggct ggtttcaaac tcctg                                             25


<210>  38
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Nanog promoter (reverse primer)

<400>  38
gacccaccct tgtgaattct cagtta                                            26


<210>  39
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Rex1 promoter (forward primer)

<400>  39
ggtttaaaag ggtaaatgtg attatattta                                        30


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide-treated Rex1 promoter (reverse primer)

<400>  40
caaactacaa ccacccatca ac                                                22


<210>  41
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Rex1 promoter (forward primer)

<400>  41
ggcctaaaag ggtaaatgtg attacaccca                                        30


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify bisulfide untreated Rex1 promoter (reverse primer)

<400>  42
caggctacag ccacccatca gc                                                22


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD133 (forward primer)

<400>  43
cttcatccac agatgctcct aag                                               23


<210>  44
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD133 (reverse primer)

<400>  44
tggattcata tgccttctgt aaga                                              24


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD44 (forward primer)

<400>  45
agggatcctc cagctccttt cg                                                22


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD44 (reverse primer)

<400>  46
cgtccgagag atgctgtagc ga                                                22


<210>  47
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD166 (forward primer)

<400>  47
gtgtgtctgg gagaagacgc tg                                                22


<210>  48
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD166 (reverse primer)

<400>  48
ggtacgtcaa gtcggcaagg tatg                                              24


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ABCB5 (forward primer)

<400>  49
atccacaagc cagactagaa ggc                                               23


<210>  50
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ABCB5 (reverse primer)

<400>  50
agatccaact gcttcctttc tcag                                              24


<210>  51
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ALDH1A1 (forward primer)

<400>  51
tgagccagtc acctgtgttc ca                                                22


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ALDH1A1 (reverse primer)

<400>  52
tggcagagct cctcctcagt tg                                                22


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD29 (forward primer)

<400>  53
atcagacgcg cagaggaggc                                                   20


<210>  54
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD29 (reverse primer)

<400>  54
gagcaaacac acagcaaact gaactg                                            26


<210>  55
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CTNNB1(forward primer)

<400>  55
acggaggaag gtctgaggag ca                                                22


<210>  56
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CTNNB1 (reverse primer)

<400>  56
tgagtagcca ttgtccacgc tgg                                               23


<210>  57
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA LGR5 (forward primer)

<400>  57
acagtgcggc agacgtaagg at                                                22


<210>  58
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA LGR5 (reverse primer)

<400>  58
ggagcagctg actgatgttg ttcatac                                           27


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD26 (forward primer)

<400>  59
gtggaaggtt cttctgggac t                                                 21


<210>  60
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD26 (reverse primer)

<400>  60
actgcccatc aggagatatt gaat                                              24


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA E-cadherin (forward primer)

<400>  61
gggtgactac aaaatcaatc                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA E-cadherin (reverse primer)

<400>  62
gggggcagta agggctcttt                                                   20


<210>  63
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Vimentin (forward primer)

<400>  63
tgaaggagga aatggctcgt c                                                 21


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Vimentin (reverse primer)

<400>  64
gtttggaaga ggcagagaaa tcc                                               23


<210>  65
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Fibronectin (forward primer)

<400>  65
ggagtttcct gagggttt                                                     18


<210>  66
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Fibronectin (reverse primer)

<400>  66
gcagaagtgt ttgggtga                                                     18


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA N-cadherin (forward primer)

<400>  67
cgaatggatg aaagacccat cc                                                22


<210>  68
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA N-cadherin (reverse primer)

<400>  68
ggagccactg ccttcatagt caa                                               23


<210>  69
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Snail (forward primer)

<400>  69
atcggaagcc taactacagc gagc                                              24


<210>  70
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Snail (reverse primer)

<400>  70
cagagtccca gatgagcatt gg                                                22


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Twist (fowrard primer)

<400>  71
ggagtccgca gtcttacgag                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Twist (reverse primer)

<400>  72
tctggaggac ctggtagagg                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ZEB1 (forward primer)

<400>  73
acccttgaaa gtgatccagc                                                   20


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ZEB1 (reverse primer)

<400>  74
cattccattt tctgtcttcc gc                                                22


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Slug (fowrard primer)

<400>  75
agatgcatat tcggacccac                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Slug (reverse primer)

<400>  76
cctcatgttt gtgcaggaga                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TGFb1 (forward primer)

<400>  77
attcctggcg atacctcagc                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TGFb1 (reverse primer)

<400>  78
acccgttgat gtccacttgc                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Sox4 (forward primer)

<400>  79
ggcctcgagc tgggaatcgc                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA Sox4 (reverese primer)

<400>  80
gcccactcgg ggtcttgcac                                                   20


<210>  81
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CEA (forward primer)

<400>  81
aacttctcct ggtctctcag ct                                                22


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CEA (reverse primer)

<400>  82
gcaaatgctt taaggaagaa g                                                 21


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA B3GALT5 (CA 19-9) (forward primer)

<400>  83
caaaccaagc ccagaacctg                                                   20


<210>  84
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA B3GALT5 (CA 19-9) (reverse primer)

<400>  84
tcaatctcat cttcgggaaa gc                                                22


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ST6GALNAC6 (CA 19-9) (forward primer)

<400>  85
gccggagatg aggaaactga                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ST6GALNAC6 (CA 19-9) (reverse primer)

<400>  86
gatcacgaac actgctgacc                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TS (forward primer)

<400>  87
acctgaatca catcgagcca                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TS (reverse primer)

<400>  88
ttggatgcgg attgtaccct                                                   20


<210>  89
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TP (forward primer)

<400>  89
cgcctggtga cttctcc                                                      17


<210>  90
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TP (reverse primer)

<400>  90
tgggtcagca ccgaggt                                                      17


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DPD (forward primer)

<400>  91
gttgtggcta tgattgatga                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DPD (reverse primer)

<400>  92
attcacagat aagggtacgc                                                   20


<210>  93
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DCC (foward primer)

<400>  93
ttccgccatg gtttttaaat ca                                                22


<210>  94
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DCC (reverse primer)

<400>  94
agcctcattt tcagccacac a                                                 21


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA KRAS (forward primer)

<400>  95
actggggagg gctttctttg                                                   20


<210>  96
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA KRAS (reverse primer)

<400>  96
ggcatcatca acaccctgtc t                                                 21


<210>  97
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TP53 (forward primer)

<400>  97
ggtggtgccc tatgagccg                                                    19


<210>  98
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA TP53 (reverse primer)

<400>  98
tcctctgtgc gccggtctc                                                    19


<210>  99
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA GPA33 (forward primer)

<400>  99
ccaatcaaag gagggctcac c                                                 21


<210>  100
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA GPA33 (reverse primer)

<400>  100
ttctcttagc tgctctggtg gc                                                22


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMI-1 (forward primer)

<400>  101
tggctcgcat tcattttctg                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMI-1 (reverse primer)

<400>  102
agtagtggtc tggtcttgtg                                                   20


<210>  103
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA APC (forward primer)

<400>  103
ggaagcagag aaagtactgg a                                                 21


<210>  104
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA APC (reverse primer)

<400>  104
ctgaagttga gcgtaatacc ag                                                22


<210>  105
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SMAD4 (forward primer)

<400>  105
ctgctgctgg aattggtgtt ga                                                22


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SMAD4 (reverse primer)

<400>  106
ctggagggcc cggtgtaagt                                                   20


<210>  107
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA AXIN2 (forward primer)

<400>  107
ctggctccag aagatcacaa ag                                                22


<210>  108
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA AXIN2 (reverse primer)

<400>  108
atctcctcaa acaccgctcc a                                                 21


<210>  109
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA POLD1 (forward primer)

<400>  109
gactacacgg gagccactgt ca                                                22


<210>  110
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA POLD1 (reverse primer)

<400>  110
gtaacacagg ttgtgggcca tc                                                22


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA STK11 (forward primer)

<400>  111
gaagtcggaa cacaaggaag                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA STK11 (reverse primer)

<400>  112
ccgtaacctc ctcagtagtt                                                   20


<210>  113
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMPR1A (forward primer)

<400>  113
gtcagaaaat ggagtaacct ta                                                22


<210>  114
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMPR1A (reverse primer)

<400>  114
tagttcgctg aaccaataaa gg                                                22


<210>  115
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA MUTYH (forward primer)

<400>  115
ggcctctgtc tccccatatc at                                                22


<210>  116
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA MUTYH (reverse primer)

<400>  116
ctgctgtagg gtctctgctg ta                                                22


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SMAD7 (forward primer)

<400>  117
ctgcaacccc catcacctta                                                   20


<210>  118
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SMAD7 (reverse primer)

<400>  118
ccctgtttca gcggaggaa                                                    19


<210>  119
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMP2 (forward primer)

<400>  119
ggtggaatga ctggattg                                                     18


<210>  120
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMP2 (reverse primer)

<400>  120
gcatcgagat agcactg                                                      17


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMP4 (forward primer)

<400>  121
ctggtccacc acaatgtgac                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA BMP4 (reverse primer)

<400>  122
cgatcggcta atcctgacat                                                   20


<210>  123
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA RHPN2 (forward primer)

<400>  123
gagaacgacg gctactttcg ga                                                22


<210>  124
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA RHPN2 (reverse primer)

<400>  124
agctcttcct tgagcatctg ca                                                22


<210>  125
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SCG5 (forward primer)

<400>  125
atatccagct caccaggcca tgaa                                              24


<210>  126
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA SCG5 (reverse primer)

<400>  126
tgttgtctcc agtcaactct gcca                                              24


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA GREM1 (forward primer)

<400>  127
gtcacactca actgccctga                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA GREM1 (reverse primer)

<400>  128
ggtgaggtgg gtttctggta                                                   20


<210>  129
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA FMN1 (forward primer)

<400>  129
cagcatctct gttcctccat ca                                                22


<210>  130
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA FMN1 (reverse primer)

<400>  130
agtgccttcc attatgccta cc                                                22


<210>  131
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-Intron to examine intron 2 (2.4kb) 
       transcriptional regulation activity of CD24 (forward primer)

<400>  131
gcgctctaga ctgcagggat tagcgcctgg ag                                     32


<210>  132
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-Intron to examine intron 2 (2.4kb) 
       transcriptional regulation activity of CD24 (reverse primer)

<400>  132
atatggccgg ccccacatca cagctacctc catgtac                                37


<210>  133
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (forward primer)

<400>  133
gatgatatcg ataccagccg ggtaccagca g                                      31


<210>  134
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (reverse primer)

<400>  134
gataagcttt caggatgctg ggtgcttgga g                                      31


<210>  135
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (forward primer)

<400>  135
gatgatatcg cagtctgagt ggcaatgcac ttg                                    33


<210>  136
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (reverse primer)

<400>  136
gataagcttt caggatgctg ggtgcttgga g                                      31


<210>  137
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (forward primer)

<400>  137
gatgatatcc ctgtagtttg cagcgtcagg ca                                     32


<210>  138
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (reverse primer)

<400>  138
gataagcttt caggatgctg ggtgcttgga g                                      31


<210>  139
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (forward primer)

<400>  139
gatgatatcc cacgcccggc caaagtattt c                                      31


<210>  140
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CD24-derived luciferase reporter to examine core 
       promoter activity of CD24 (reverse primer)

<400>  140
gataagcttt caggatgctg ggtgcttgga g                                      31


<210>  141
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing a Sox2 binding site (forward primer)

<400>  141
tggcaggtcc cgggaaacaa aggaaacttg ggcccggc                               38


<210>  142
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   CD24 promoter region bearing a Sox2 binding site (reverse 
       primer)

<400>  142
gccgggccca agtttccttt gtttcccggg acctgcca                               38


<210>  143
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing two Sox2 binding sites (forward 
       primer)

<400>  143
ccgggaaaca aaggaaactc cgggaaacaa aggaaact                               38


<210>  144
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing two Sox2 binding sites (reverse 
       primer)

<400>  144
agtttccttt gtttcccgga gtttcctttg tttcccgg                               38


<210>  145
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing no Sox2 binding site (none relevant 
       sequence 1) (forward primer)

<400>  145
ccagccgggt accagcagcc ggcggcgccc gcccacct                               38


<210>  146
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing no Sox2 binding site (none relevant 
       sequence 1) (reverse primer)

<400>  146
aggtgggcgg gcgccgccgg ctgctggtac ccggctgg                               38


<210>  147
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing no Sox2 binding site (none relevant 
       sequence 2) (forward primer)

<400>  147
gctttcctgg tatataaggt ctcgccggct cgccgcgc                               38


<210>  148
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing no Sox2 binding site (none relevant 
       sequence 2) (reverse primer)

<400>  148
gcgcggcgag ccggcgagac cttatatacc aggaaagc                               38


<210>  149
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing a mutated Sox2 binding site (forward
       primer)

<400>  149
tggcaggtcc cgggaaaagg aggaaacttg ggcccggc                               38


<210>  150
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing a mutated Sox2 binding site (reverse
       primer)

<400>  150
gccgggccca agtttcctcc ttttcccggg acctgcca                               38


<210>  151
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing a consensus Sox2 binding site 
       (positive control) (forward primer)

<400>  151
tggcaggtcc atttgcataa caaagagttg ggcccggc                               38


<210>  152
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD24 promoter region bearing a consensus Sox2 binding site 
       (positive control) (reverse primer)

<400>  152
gccgggccca actctttgtt atgcaaatgg acctgcca                               38


<210>  153
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CRISPR to knockout miR205 binding site on CD24 3'-UTR 
       (forward primer)

<400>  153
aaacgagttt catgtacaag atgagt                                            26


<210>  154
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CRISPR to knockout miR205 binding site on CD24 3'-UTR 
       (reverse primer)

<400>  154
taaaactcat cttgtacatg aaactc                                            26


<210>  155
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 assay to quantify CRISPR-mediated cleavage efficiency on 
       targeted CD24 site (forward primer)

<400>  155
cagtcaacag ccagtctctt cgtg                                              24


<210>  156
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 assay to quantify CRISPR-mediated cleavage efficiency on 
       targeted CD24 site (reverse primer)

<400>  156
catcattgcc ctggcacatg tca                                               23


<210>  157
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct left homologous arm bearing CD24 sequences (forward 
       primer)

<400>  157
gcgctacgta ccaggataga cagtgaccca atga                                   34


<210>  158
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct left homologous arm bearing CD24 sequences (reverse 
       primer)

<400>  158
atatgtcgac cttgtacatg aaactccagc agat                                   34


<210>  159
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct right homologous arm bearing CD24 sequences (forward 
       primer)

<400>  159
atatgcggcc gcgaaggaga ggcaacatcc aaaatag                                37


<210>  160
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct right homologous arm bearing CD24 sequences (reverse 
       primer)

<400>  160
gcgcttcgaa tcaagcctgt aatcccagca ctttg                                  35


<210>  161
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of mCherry transgene on CD24 
       (forward primer)

<400>  161
gacatcacct cccacaacga ggact                                             25


<210>  162
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of mCherry transgene on CD24 
       (reverse primer)

<400>  162
agcatcagtg tgtgaccatg cgaac                                             25


<210>  163
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome 6 (forward primer)

<400>  163
gagcaatggt ggccaggctc                                                   20


<210>  164
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome 6 (reverse primer)

<400>  164
ggattgggtt tagaagatgg ggaaa                                             25


<210>  165
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome Y (forward primer)

<400>  165
gagcaatggt ggccaggctt                                                   20


<210>  166
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome Y (reverse primer)

<400>  166
ggattgggtt tagaagatgg ggaag                                             25


<210>  167
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome 15 (forward primer)

<400>  167
atccagccag acacgctgca                                                   20


<210>  168
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 on chromosome 15 (reverse primer)

<400>  168
cagtagctgg atttggggca gtc                                               23


<210>  169
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify truncated mRNA CD24 expressed by chromosome 6 (forward 
       primer)

<400>  169
tctggaagtc caatgtggca ag                                                22


<210>  170
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify truncated mRNA CD24 expressed by chromosome 6 (reverse 
       primer)

<400>  170
cactggaagt tcccttctca tgtac                                             25


<210>  171
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify precursor stem-loop hsa-mir-205 expression (forward 
       primer)

<400>  171
agatcctcag acaatccatg tgct                                              24


<210>  172
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify precursor stem-loop hsa-mir-205 expression (reverse 
       primer)

<400>  172
agctccatgc ctcctgaact                                                   20


<210>  173
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA synthesis of mature miR205-5p (adapter)

<400>  173
tccgtcgttc taatgcgaac agactccg                                          28


<210>  174
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify mature miR205-5p  expression (forward primer)

<400>  174
cctccatcct tcattccacc g                                                 21


<210>  175
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify mature miR205-5p  expression (reverse primer)

<400>  175
gtttccgtcg ttctaatgcg aa                                                22


<210>  176
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify miR205HG expression (forward primer)

<400>  176
ctcaagtacc catcttggag gg                                                22


<210>  177
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quantify miR205HG expression (reverse primer)

<400>  177
cacgcacact ccagatgtct c                                                 21


<210>  178
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CMV-miR205 (forward primer)

<400>  178
gcgcgaattc aaagatcctc agacaatcca tgtgct                                 36


<210>  179
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CMV-miR205 (reverse primer)

<400>  179
atatactagt tgtcagctcc atgcctcctg aac                                    33


<210>  180
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CMV-miR205HG (co-expressing miR205) (forward primer)

<400>  180
gcgcgaattc gaaatgggct gagtccctct tg                                     32


<210>  181
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CMV-miR205HG (co-expressing miR205) (reverse primer)

<400>  181
atatactagt tttttcagta gacaagcaac ttttag                                 36


<210>  182
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-UTR to examine 3'-UTR (211bp) transcriptional 
       regulation activity of CD24 (forward primer)

<400>  182
gcgctctaga cttaagagac tcaggccaag aaacg                                  35


<210>  183
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-UTR to examine 3'-UTR (211bp) transcriptional 
       regulation activity of CD24 (reverse primer)

<400>  183
atatggccgg ccggcatcca tcatctagtc aaacctc                                37


<210>  184
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-UTR to examine 3'-UTR transcriptional 
       regulation activity of CD24 (forward primer)

<400>  184
gcgctctaga cttaagagac tcaggccaag aaacg                                  35


<210>  185
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct PGK-Luc-UTR to examine 3'-UTR transcriptional 
       regulation activity of CD24 (reverse primer)

<400>  185
atatggccgg cccaagccac attcaaggaa atcatgtct                              39


<210>  186
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CRISPR to knockout CD24 ORF (forward primer)

<400>  186
aaacggacat gggcagagca atgggt                                            26


<210>  187
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct CRISPR to knockout CD24 ORF (reverse primer)

<400>  187
taaaacccat tgctctgccc atgtcc                                            26


<210>  188
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 assay to quantify CRISPR-mediated cleavage efficiency on 
       targeted CD24 site (forward primer)

<400>  188
cacgtcacgg ctattgtggc tttc                                              24


<210>  189
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7E1 assay to quantify CRISPR-mediated cleavage efficiency on 
       targeted CD24 site (reverse primer)

<400>  189
gcctctgggt gaaagtggga agtag                                             25


<210>  190
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct left homologous arm bearing CD24 sequences (forward 
       primer)

<400>  190
gcgctacgta gctcacagaa caaagcaagg gcttc                                  35


<210>  191
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct left homologous arm bearing CD24 sequences (reverse 
       primer)

<400>  191
atatgtcgac ttgctctgcc catgtcccct                                        30


<210>  192
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct right homologous arm bearing CD24 sequences (forward 
       primer)

<400>  192
atatgcggcc gctggtggcc aggctcgggc t                                      31


<210>  193
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct right homologous arm bearing CD24 sequences (reverse 
       primer)

<400>  193
gcgcttcgaa aggatctagg gagaccgcgc tggtag                                 36


<210>  194
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of mCherry transgene on CD24 
       (forward primer)

<400>  194
gacatcacct cccacaacga ggact                                             25


<210>  195
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Check for site-specific integration of mCherry transgene on CD24 
       (reverse primer)

<400>  195
atcctccaaa cccgaactga ccca                                              24


<210>  196
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 (fowrad primer)

<400>  196
gagcaatggt ggccaggctc                                                   20


<210>  197
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA CD24 (reverse primer)

<400>  197
ggattgggtt tagaagatgg ggaaa                                             25


<210>  198
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DPP4 (forward primer)

<400>  198
cattcacttt tgaaaagttc tccctagaaa g                                      31


<210>  199
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA DPP4 (reverse primer)

<400>  199
cgtttttgtg cagttttaaa atgtgtgc                                          28


<210>  200
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ITGB1 (forward primer)

<400>  200
ggttctcata tgaacctaac tggtcaaa                                          28


<210>  201
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA ITGB1(reverse primer)

<400>  201
atcatgttta taaaagcaat agaaggtacg gt                                     32


<210>  202
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA KIT (forward primer)

<400>  202
aaggtgatct atttttccct ttctcc                                            26


<210>  203
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA KIT (reverse primer)

<400>  203
tttcatactg accaaaactc agcct                                             25


<210>  204
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA MET (forward primer)

<400>  204
gactcctaca acccgaatac tgc                                               23


<210>  205
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplify mRNA MET (reverse primer)

<400>  205
catagtgctc cccaatgaaa gtagag                                            26


