

SEQUENCE LISTING

<110> VILNIUS UNIVERSITY
 
<120> PROGRAMMABLE RNA SHREDDING BY THE TYPE III-A CRISPR-CAS SYSTEM OF STREPTOCOCCUS THERMOPHILUS

<130> 

<140> PCT/IB2015/056756
<141> 2015-09-04

<150> 62/046,384
<151> 2014-09-05

<160> 1    

<170> PatentIn version 3.5

<210> 1
<211> 11543 base pairs
<212> DNA
<213> Streptococcus thermophilus
      Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
      Streptococcus.

<400> 1


LOCUS       KM222358               11543 bp    DNA     linear   BCT 10-DEC-2014
DEFINITION  Streptococcus thermophilus strain DGCC8004 dihydroorotate
            dehydrogenase (pyrD) gene, partial cds; Cas1 protein (cas1), Cas2
            protein (cas2), Cas6 protein (cas6), Cas10 protein (cas10), Csm2
            protein (csm2), Csm3 protein (csm3), Csm4 protein (csm4), Csm5
            protein (csm5), Csm6' protein (csm6'), and Csm6 protein (csm6)
            genes, complete cds; and orotidine-5'-phosphate decarboxylase
            (pyrF) gene, partial cds.
ACCESSION   KM222358
VERSION     KM222358.1  GI:729057608
KEYWORDS    .
SOURCE      Streptococcus thermophilus
  ORGANISM  Streptococcus thermophilus
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Tamulaitis,G., Kazlauskiene,M., Manakova,E., Venclovas,C.,
            Nwokeoji,A.O., Dickman,M.J., Horvath,P. and Siksnys,V.
  JOURNAL   Mol. Cell 56 (4), 506-517 (2014)
   PUBMED   25458845
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Tamulaitis,G., Kazlauskiene,M. and Horvath,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2014) Institute of Biotechnology, Vilnius
            University, Graiciuno 8, Vilnius, LT 02241, Lithuania
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Streptococcus thermophilus"
                     /mol_type="genomic DNA"
                     /strain="DGCC8004"
                     /db_xref="taxon:1308"
     gene            <1..99
                     /gene="pyrD"
     CDS             <1..99
                     /gene="pyrD"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="AIZ03600.1"
                     /db_xref="GI:729057609"
                     /translation="KIIDRLPEVMDKYGITTLEDLRKEVRTDLLGK"
     gene            238..1242
                     /gene="cas1"
     CDS             238..1242
                     /gene="cas1"
                     /codon_start=1
                     /transl_table=11
                     /product="Cas1 protein"
                     /protein_id="AIZ03601.1"
                     /db_xref="GI:729057610"
                     /translation="MSDLYSQRSNYYLSLSEQRIIIKNDNKEIVKEVSISLVDNVLLF
                     GNAQLTTQLIKALSKNKMNGYYFSNVGQFISSIETHRQDEFQKQELQAKAYFEEDFRL
                     EVARSIATTKVRHQIALLREFDTDGLLDTSDYSRFEDSVNDIQKAYSITEIMGYEGRL
                     AKSYFYYLNLLVPDDFHFNGRSRRPAEDCFNSALNFGYSILYSCLMGLIKKNGLSLGF
                     GVIHKHHQHHATLASDLIEEWRPIIVDNTLMELIRNGKLLLSHFENKDQDFILTDEGR
                     EIFARALRSRILEVHQYIELDKKRYSFLYTADRQIKSLIRAFRELDLSLYETSYTGGH
                     "
     gene            1242..1571
                     /gene="cas2"
     CDS             1242..1571
                     /gene="cas2"
                     /codon_start=1
                     /transl_table=11
                     /product="Cas2 protein"
                     /protein_id="AIZ03602.1"
                     /db_xref="GI:729057611"
                     /translation="MGLYFNLSEEEREFAKQKTMFCLIIYDIRSNKRRLKLSKLLEGY
                     GVRVQKSCFEVDLSRNDYQSLLKDIEGFYKADEEDSIIVYVTTKEEVTSFSPYHSAEK
                     LDDILFF"
     repeat_region   1672..2661
                     /note="CRISPR2 array type III-A"
     gene            2792..3523
                     /gene="cas6"
     CDS             2792..3523
                     /gene="cas6"
                     /codon_start=1
                     /transl_table=11
                     /product="Cas6 protein"
                     /protein_id="AIZ03603.1"
                     /db_xref="GI:729057612"
                     /translation="MKKLVFTFKRIDHPAQDLAVKFHGFLMEQLDSDYVDYLHQQQTN
                     PYATKVIQGKENTQWVVHLLTDDHEDKVFMTLLQIKEVSLNDLPKLSVEKVEIQELGA
                     DKLLEIFNSEENQTYFSIIFETPTGFKSQGSYVIFPSMRLIFQSLMQKYGRLVENQPE
                     IEEDTLDYLSEHSTITNYRLETSYFRVHRQRIPAFRGKLTFKVQGAKTLKAYVKMLLT
                     FGEYSGLGMKTSLGMGGIKLEERKD"
     gene            3504..5780
                     /gene="cas10"
     CDS             3504..5780
                     /gene="cas10"
                     /codon_start=1
                     /transl_table=11
                     /product="Cas10 protein"
                     /protein_id="AIZ03604.1"
                     /db_xref="GI:729057613"
                     /translation="MKKEKIDLFYGALLHDIGKVIQRATGERKKHALVGADWFDEIAD
                     NQVISDQIRYHMANYQSDKLGNDHLAYITYIADNIASGVDRRQSNEESDEDASAKIWD
                     TYTNQADIFNVFGAQTDKRYFKPTVLNLKSKPNFASATYEPFSKGDYAAIATRIKNEL
                     AEFEFNQAQIDSLLNLFEAILSFVPSSTNSKEIADISLAEHSRLTAAFALAIYDYLED
                     KGRHNYKEDLFTKASAFYEEEAFLLASFDLSGIQDFIYNIATSGAAKQLKARSLYLDF
                     MSEYIADSLLDKLGLNRANLLYVGGGHAYFVLANTEKTVETLVQFEKDFNQFLLANFQ
                     TRLYVAFGWGSFAAKDIMSELNSPESYRQIYQKASRMISEKKISRYDYRTLMLLNRGG
                     KSSERECEICHSVENLVSYHDQKVCDICRGLYQFSKEIAHDHFIITENEGLPIGPNAC
                     LKGVAFEKLSQESFSRVYVKNDYKAGTIKATHVFVGDYQCDEIHKYAALSKNEDGLGI
                     KRLAVVRLDVDDLGAAFMAGFSRQGNGQYSTLSRSATFSRSMSLFFKVYINQFASDKK
                     LSIIYAGGDDVFAIGSWQDIIAFTVELRQNFIKWTNGKLTLSAGIGLFADKTPISLMA
                     HQTGELEEAAKGNEKDSISLFSSDYTFKFDRFITNVYDDKLEQIRYFFNHQDERGKNF
                     IYKLIELLRNYESEEKMNVARLAYYLTRLEELTDKDERDKFKQFKKLFFKWYTNNESD
                     RKEAELALLLYVYEIRKD"
     gene            5784..6149
                     /gene="csm2"
     CDS             5784..6149
                     /gene="csm2"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm2 protein"
                     /protein_id="AIZ03605.1"
                     /db_xref="GI:729057614"
                     /translation="MAILTDENYVDKAERAISLLEKDNKGNYLLTTSQIRKLLSLCSS
                     LYDRSKERKFDELINDVSYLRVQFVYQAGREIAVKDLIEKAQILEALKEIKDRETLQR
                     FCRYMEALVAYFKFYGGKD"
     gene            6149..6811
                     /gene="csm3"
     CDS             6149..6811
                     /gene="csm3"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm3 protein"
                     /protein_id="AIZ03606.1"
                     /db_xref="GI:729057615"
                     /translation="MTFAKIKFSAQIRLETGLHIGGSDAFAAIGAIDSPVIKDPITNI
                     PIIPGSSLKGKMRTLLAKVYNEKVAEKPSDDSDILSRLFGNSKDKRFKMGRLIFRDAF
                     LSNADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFELIYEITDENE
                     NQVEEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFEKLKATTVFGNYDVKTLNELLTA
                     EV"
     gene            6813..7712
                     /gene="csm4"
     CDS             6813..7712
                     /gene="csm4"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm4 protein"
                     /protein_id="AIZ03607.1"
                     /db_xref="GI:729057616"
                     /translation="MTYKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLESLKMG
                     KLDAFLAEANQDKFTLTDAFPFQFGPFLPKPIGYPKHDQIDQSVDVKEVRRQAKLSKK
                     LQFLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDGNLYQVATTRFSNDTSLYVIA
                     NESDLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSDRLTKNHSDKVMSLTTA
                     LPVDADLEEAMEDGHYLLTKSSGFAFSHATNENYRKQDLYKFASGSTFSKTFEGQIVD
                     VRPLDFPHAVLNYAKPLFFKLEV"
     gene            7715..8788
                     /gene="csm5"
     CDS             7715..8788
                     /gene="csm5"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm5 protein"
                     /protein_id="AIZ03608.1"
                     /db_xref="GI:729057617"
                     /translation="MKNDYRTFKLSLLTLAPIHIGNGEKYTSREFIYENKKFYFPDMG
                     KFYNKMVEKRLAEKFEAFLIQTRPNARNNRLISFLNDNRIAERSFGGYSISETGLESD
                     RNPNSAGAINEVNKFIRDAFGNPYIPGSSLKGAIRTILMNTTPKWNNENAVNDFGRFP
                     KENKNLIPWGPKKGKEYDDLFNAIRVSDSKPFDNKRLILVQKWDYSAKTNKAKPLPLY
                     RESISPLTKIEFEITTTTDEAGRLIEELGKRAQAFYKDYKAFFLSEFPDDKIQANLQY
                     PIYLGAGSGAWTKTLFKQADGILQRRYSRMKTKMVKKGVLKLTKAPLKIVKIPSGNHS
                     LIKNHESFYEMGKANFMIKEIDK"
     gene            8785..9945
                     /gene="csm6'"
     CDS             8785..9945
                     /gene="csm6'"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm6' protein"
                     /protein_id="AIZ03609.1"
                     /db_xref="GI:729057618"
                     /translation="MRVLISAVGDTDPFRNFHDGSLIHIARKYRPEKVILIFSEHTAK
                     KQGNIEKALFSIAPNYEPELIIHDPIISDNEVHIFDVMFQRFSDILQEYYTKEDEFIL
                     NLSSATPQIKSALFVINRLNGINVKAVQVSSPEHASNENIGHDNDENIDELIEVNKDN
                     KVNFIDRTIEDNAEKFSQALLKKTARDFIEKFDYKAALDILDQLSDFPNLKSVREEIR
                     DVVNCLSKQDVPKGLRHKKLKEEEQKILSAYLTIELQRERGNVSESFIRIKNLTEFIL
                     EDYIEKRYPGLIDEYCEDIQKYYLSLFDYSKLLKATKEFKLKRTIAPIIDMNSSRNKV
                     AHSLSPLDSDAVKQLGIAMKTLKTLVREQYHFSQSDFNFYHDLNKILLTKLN"
     gene            9960..11246
                     /gene="csm6"
     CDS             9960..11246
                     /gene="csm6"
                     /codon_start=1
                     /transl_table=11
                     /product="Csm6 protein"
                     /protein_id="AIZ03610.1"
                     /db_xref="GI:729057619"
                     /translation="MKILISAVGTTDPISNNHDAALLHIARNYRPDKIVLVYSQEMMV
                     KQDLINKVLLSIEGYNPIIEIDSTILNNDEVFLFDKMYEVMGQIVQKYTNDDNEIILN
                     LSSGTPQIISALFALNRINDYNTQAIQVATPKNRANREYTALTESEIDALIMENQDNR
                     LDFVDRSIKDKSEKFTQALVKRHLRSLIASFDYQAAEAIINRKEYNKLLSKKKIAYIR
                     EKLYDFSRVFKNQSILSDILSFPLDDSQKKALNYYLMIDVLKEREHIADVLIKAKSLA
                     EFVIEETIKKDHEGLIVFDGNLPKLNPSFPDCEAILDDIDKKMKKSRGIEDTEERIFS
                     VQSTLNLLSYLNILEFYEYDSQLQTAINGILSLNGERNKVAHGLSEIDTRLLSRKKLK
                     QLSENLRLLLVDCLGIDSSYFNYYDKQNKELIKMLE"
     gene            11445..>11543
                     /gene="pyrF"
     CDS             11445..>11543
                     /gene="pyrF"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="AIZ03611.1"
                     /db_xref="GI:729057620"
                     /translation="MSEKRPVIALDFPTFEDVKAFLVKFPADEKLYV"
ORIGIN      
        1 aaaatcattg accgcctccc agaagtcatg gacaagtatg gcatcacaac actagaagac
       61 ttacgtaagg aagttcgaac agacttgttg gggaaataag gaaagcaatc tctttacaat
      121 aaataaaaat cgaagtccta tcgttagggc ttcatttttt agataagaca ttagaatata
      181 ttgacgatta gaattgtggt gatataatat atgtcataaa atactctgag gtagcttatg
      241 agcgatttat atagtcaaag gtccaattat tacctatcct tatctgaaca aagaattatc
      301 attaaaaatg ataataaaga gattgtcaaa gaagtgtcca tttcactcgt tgataatgta
      361 ttactttttg gtaatgcaca actgaccacc caactcatca aagccttgtc aaagaacaag
      421 atgaatggtt actatttctc aaatgttggt caatttattt ctagtattga aacccacagg
      481 caggacgaat tccaaaagca agagttgcaa gcaaaggctt attttgaaga ggatttccgt
      541 ttagaggttg cgaggagtat tgctacgacc aaggtgaggc accagattgc cttacttaga
      601 gagtttgata cggatggtct actagatacc tcagattatt ctaggtttga agatagtgtc
      661 aatgatattc agaaagctta ttccattaca gaaattatgg gttacgaagg tcgccttgcg
      721 aaatcctatt tttactatct gaatttactc gttcctgatg actttcattt taatggtagg
      781 agtagacggc ctgcggagga ttgttttaac agtgccctca attttggcta tagtatctta
      841 tattcttgct taatggggct gattaagaaa aatgggctaa gcttgggatt tggggtaatt
      901 cacaagcatc atcagcatca tgcgaccttg gccagtgatt taatagaaga atggagacct
      961 atcatcgtcg ataatacgct tatggagttg atacgaaatg gtaaacttct tttaagtcat
     1021 tttgaaaata aggatcaaga cttcatactc accgatgaag gcagagaaat ctttgcacgg
     1081 gctttacgtt caagaatatt agaagtccat cagtatattg agttagacaa aaaacgctat
     1141 tcttttcttt atacagcaga taggcaaatc aagagtttga ttagggcttt tagagaactt
     1201 gaccttagtc tctatgagac aagttacaca ggagggcatt aatgggactt tactttaacc
     1261 tcagcgaaga agagcgtgag tttgccaaac aaaaaaccat gttttgtctg attatttatg
     1321 atatccgaag taacaaacgt agacttaaac tctcgaaatt acttgagggt tatggcgtga
     1381 gggtgcaaaa atcctgtttc gaggtcgacc tgtcaagaaa tgattatcag tctctcctta
     1441 aggatatcga gggcttctac aaggctgatg aagaagacag cataatagtg tatgtgacaa
     1501 ccaaagaaga ggtgactagt tttagcccct accatagtgc tgaaaaatta gatgacattc
     1561 tcttcttcta agcctttata gacctttaat catatggtac actatagata gtgtttccag
     1621 taggtcctac atcttgtgcc tctagcaact gcctagagca caagatatgg ggatataaac
     1681 ctaattacct cgagagggga cggaaacttt tcttgccata tcaacaaaat catcaaattg
     1741 tttgatataa acctaattac ctcgagaggg gacggaaact taattttagt atcaccatca
     1801 atgtttgcat aaccagtaga tataaaccta attacctcga gaggggacgg aaactttcgt
     1861 aactgtttaa ttctgttcac ttattccacc gatataaacc taattacctc gagaggggac
     1921 ggaaactaac ctggtgcata tataccaacc agtttgtctt taacattttg atataaacct
     1981 aattacctcg agaggggacg gaaactctta aatcaatgtc ttacctaaac cgtgaatttt
     2041 gatataaacc taattacctc gagaggggac ggaaacaaca tattatttat ctccatatat
     2101 ttttttcttt ttggatataa acctaattac ctcgagaggg gacggaaacg cgttaccgta
     2161 gacctctgcg ttaccactta cctgatataa acctaattac ctcgagaggg gacggaaact
     2221 gttccaattt agtagcatta atgaatctgt acttatctaa gatataaacc taattacctc
     2281 gagaggggac ggaaacacta gtagattttt catattaatc tacctccttt ctataatgat
     2341 ataaacctaa ttacctcgag aggggacgga aacttgtcct tgaaaagcaa ttcacctttg
     2401 ttaaattgat ataaacctaa ttacctcgag aggggacgga aacgctttct agctcgctat
     2461 aattacccat tcctagaaag atataagcct aattacctcg agaggggacg gaaactcttt
     2521 cgacttagaa tcgtattcaa atttcatgtt tttgatataa acctaattac ctcgagaggg
     2581 gacggaaact taacgaagtt gtcatcaatc attactcctt gcctggatat aaacctaatt
     2641 acctcgagag gggacttttt tgaaaatttt gaaaacaata ttgacaccgc ttccagaaag
     2701 tgttagacta aaagcacatt aagggcgccc caatgagttg aaaagtactt tcagcttttg
     2761 ggggtttttc atacaaagat gaaggagtcg aatgaaaaaa ttagtattta cttttaaaag
     2821 gatcgaccat cctgcacaag atttggctgt taaatttcat ggcttcttga tggagcagtt
     2881 ggatagtgac tatgttgatt atctgcatca gcagcaaaca aatccctatg cgaccaaggt
     2941 aatccaaggg aaagaaaaca cgcagtgggt tgtacatctg ctcacagatg accacgagga
     3001 taaggttttt atgaccttat tacagattaa agaggtgtcc ttaaacgatc tgcctaaact
     3061 cagtgtcgaa aaagttgaga ttcaggagtt gggggcagat aaactgttag agattttcaa
     3121 tagcgaggaa aatcaaacct atttttcaat tatttttgag actccaacag gttttaaatc
     3181 tcaaggttcc tacgtcatct tcccatctat gcgtttgatt tttcaaagtt tgatgcaaaa
     3241 gtatggaagg ttggtggaaa atcaacctga aattgaagag gataccttag attacctatc
     3301 tgaacacagc actatcacga attatcgctt ggagacgagt tatttcaggg tgcacaggca
     3361 acgaattcct gcctttagag gaaagttaac ctttaaagta caaggcgcca aaactctaaa
     3421 agcttatgtc aaaatgcttc taacattcgg tgaatattca ggtcttggca tgaaaacgag
     3481 tctcggtatg ggagggataa agcttgaaga aagaaaagat tgatttattt tacggagctc
     3541 ttttgcatga tatcggtaag gtcattcaaa gggcgacagg agaacgaaaa aaacacgcct
     3601 tggtaggcgc ggattggttt gatgagattg ctgataatca agttatttcc gatcaaatta
     3661 gatatcacat ggctaactac cagagtgata aacttggaaa tgaccatctt gcttacataa
     3721 cttatatcgc tgataacatt gcctctggtg tcgacagaag acagtcaaat gaggagagtg
     3781 acgaggatgc atcagctaag atttgggata cctatacaaa ccaggctgat atttttaacg
     3841 tttttggggc acaaacggat aaacgctact ttaaaccgac ggttctaaac ttgaaatcta
     3901 aacctaactt tgcgtcggca acatatgaac ctttctcaaa aggtgattat gcggcaattg
     3961 cgactcgtat caaaaatgaa ttggcagaat ttgagtttaa tcaagcacaa attgactctt
     4021 tgttaaatct gttcgaagca atcctctctt ttgtgccttc ttcgactaat tctaaagaaa
     4081 tcgctgatat ttcacttgct gagcatagtc gtctgacagc agcttttgct ctagccatct
     4141 atgattactt ggaagacaaa ggtcgtcata actataagga ggacttgttt actaaagcat
     4201 cagcctttta tgaggaagaa gcttttctcc tagctagctt tgacttatca gggattcaag
     4261 actttatcta taatattgcg acgagtggtg ctgctaaaca attgaaggct agatctttat
     4321 atcttgactt tatgagcgag tatatagcag acagtttact tgataaacta ggcctcaatc
     4381 gggctaattt gctctatgtc ggtgggggac atgcttactt tgtcctagcc aatactgaaa
     4441 aaacggtaga aacactcgtt caatttgaaa aagatttcaa tcaattttta ttggcaaatt
     4501 tccaaaccag attatatgtt gcctttggtt ggggaagctt tgcggctaag gatatcatga
     4561 gcgaactaaa ctcacctgaa agctatagac agatctatca aaaggctagt cgcatgattt
     4621 ctgagaaaaa aatctcaagg tatgattatc gaacccttat gttgttgaac aggggcggta
     4681 aatcttctga aagagagtgc gagatttgtc attccgttga gaatttagtt tcttatcatg
     4741 accaaaaagt gtgtgacatt tgtcgaggct tgtatcaatt ttctaaagag attgcccatg
     4801 accatttcat tatcactgaa aatgagggac ttcctattgg accgaatgca tgtcttaaag
     4861 gtgttgcatt tgaaaagcta agccaagaat ctttctcccg tgtctatgtc aaaaatgact
     4921 ataaggctgg tacaattaag gcaactcatg ttttcgttgg agattaccag tgtgatgaaa
     4981 tacacaaata tgctgccttg tctaaaaatg aagacggttt aggtattaaa cgtttagctg
     5041 ttgtacgtct tgacgtggat gatttgggag ctgcttttat ggcaggcttc tcccgacaag
     5101 gaaatgggca atatagtact ctatcacgct cagccacttt ctctcgaagc atgagtcttt
     5161 tcttcaaggt ttatattaac cagtttgcta gtgataagaa gctctctatc atctatgccg
     5221 gtggggacga tgtttttgcc atcggctcat ggcaagatat tattgccttt actgttgaac
     5281 tccgtcagaa cttcattaaa tggacaaatg gaaaactaac actctcggct ggtatcggtc
     5341 tgtttgctga taagacccct attagcttaa tggcacatca aacaggggag ctagaagaag
     5401 cagctaaagg caatgagaaa gatagtattt cactctttag ttctgactat acctttaaat
     5461 ttgatcggtt tatcactaat gtttacgacg ataagttaga gcagattcgc tatttcttta
     5521 atcaccaaga tgaacgaggt aagaatttca tttataaatt gattgaattg cttcgaaact
     5581 acgagagtga agaaaagatg aatgttgccc gattagctta ctatttgaca cgtctggaag
     5641 agttaacaga caaagatgaa agagataaat tcaaacaatt caagaaatta ttcttcaaat
     5701 ggtatacaaa caatgaatca gaccgtaaag aggcggaatt agctttgctt ctctatgttt
     5761 atgaaattag gaaggattaa tacatggcaa ttttaactga tgagaattat gtagataaag
     5821 cagaacgtgc tatttcatta cttgaaaagg ataataaagg gaattattta ctaacaacgt
     5881 cacaaattag aaagttatta tccttatgta gttctttata tgatagaagt aaagaaagaa
     5941 agtttgatga attaataaat gatgtttcct atttaagagt acaatttgtc tatcaagcag
     6001 gtagagagat tgcagtaaaa gatctgatag aaaaggctca aattcttgag gctcttaagg
     6061 aaatcaaaga tagagagaca cttcaaagat tttgtagata tatggaagca ttagtagcct
     6121 atttcaagtt ttatggaggt aaagattaat gacattcgct aagattaaat tttcagctca
     6181 aattcgttta gagacaggcc tccatattgg tggaagcgat gcttttgcag ccattggtgc
     6241 aatcgattcg cctgttatta aagatcctat taccaacata ccgatcattc ctggttcaag
     6301 tctcaaagga aaaatgagaa cgcttcttgc caaggtttat aatgaaaagg tagctgagaa
     6361 accaagcgat gacagtgata ttcttagccg tttatttggg aatagtaaag ataaacgatt
     6421 caaaatggga cgcttgattt ttcgtgatgc cttcttgtca aacgctgatg agctagactc
     6481 tcttggggta agaagttata cagaagtaaa atttgaaaat acaattgacc gtatcactgc
     6541 cgaagctaat ccaagacaaa ttgaacgtgc tattcgtaac agtacttttg atttcgagtt
     6601 gatttatgaa attacagatg agaatgaaaa tcaagtcgaa gaagatttca aagtgattcg
     6661 agatggttta aaactgcttg aacttgatta tcttggtggt tctggatctc gaggttacgg
     6721 taaggttgct tttgaaaaac tcaaagctac taccgtattt ggtaattatg atgttaaaac
     6781 attaaatgaa cttttaactg cggaggtcta atatgaccta taaactgtat attatgacct
     6841 ttcagaatgc tcattttggt tcgggcactc ttgatagctc aaaattaaca ttctcagcag
     6901 accgtatctt ctcagcacta gtgctagaat ccctaaaaat gggaaaactc gatgcatttc
     6961 ttgcggaagc taaccaagac aagttcacgc tcacagatgc ctttccattt caatttggtc
     7021 cctttttgcc gaaacctatt ggttatccca aacatgacca aatagatcaa tcagttgatg
     7081 tcaaagaggt tcgccgtcaa gcaaaattgt ctaagaaact gcaatttctt gctctagaaa
     7141 atgttgacga ttatctcaat ggagagttat ttgaaaatga agagcatgca gtcattgata
     7201 ctgtgacaaa aaatcaacca cataaggatg gcaatcttta tcaggtagct acaactagat
     7261 tttcaaatga tacgtcgctt tacgtcatcg caaacgaatc tgatttgctt aatgagttga
     7321 tgtctagtct tcagtattca ggtcttggtg gaaagcgttc aagtggtttt ggtcgttttg
     7381 agttagatat tcaaaatatc ccactagaat tgtcagatag actgactaag aatcattcag
     7441 ataaagtgat gagtcttacg acagcacttc ctgtagatgc tgaccttgaa gaagcaatgg
     7501 aagatggaca ttacttatta actaaatcaa gtggttttgc atttagtcat gctaccaatg
     7561 agaattatcg taagcaggat ctttacaaat ttgcttctgg ttcaactttt agtaaaacat
     7621 ttgaaggtca gattgttgat gtgagaccac ttgatttccc tcatgctgtt ttaaattatg
     7681 ctaaaccact cttctttaaa ttggaggtat aaaaatgaaa aatgactata gaacatttaa
     7741 attaagcctc ctgacacttg ctccaattca tattggtaat ggagagaagt atacctctag
     7801 agaatttatc tatgaaaata agaagtttta ctttcctgac atggggaaat tctataataa
     7861 aatggtggag aagaggcttg ctgaaaagtt tgaagcattt ctaattcaaa ctcgtccaaa
     7921 tgcacgtaat aatcgtctta tttccttctt aaatgataac cgaattgcag agcgttcttt
     7981 tggaggttat agtatctctg aaacaggttt agaatcggac agaaatccta attcagccgg
     8041 agctattaac gaagttaata agtttattcg agatgctttt ggaaatccct acattcctgg
     8101 tagctcacta aaaggtgcta ttcgtaccat tttaatgaat actaccccta agtggaataa
     8161 tgaaaatgct gtaaatgact ttggaagatt tccgaaagag aataagaacc ttatcccttg
     8221 gggaccaaaa aagggaaaag aatacgatga tttgtttaac gcaattcgtg tgagtgatag
     8281 taagcctttt gataataagc gtcttatctt agtgcagaaa tgggattatt cagcgaaaac
     8341 aaataaagct aaaccacttc ccttgtatag agaatcaatc tctccattaa caaaaattga
     8401 atttgagatt acaacaacca ctgatgaagc tggaagattg attgaagaat taggtaagag
     8461 agcacaagcg ttttataaag actataaggc atttttccta tctgaatttc ctgatgataa
     8521 gattcaagcc aatctacaat acccaattta tttaggtgcg gggagcggtg cttggacaaa
     8581 gactctattt aagcaagctg atggtatttt acaaagacga tacagtcgaa tgaaaactaa
     8641 aatggttaaa aaaggagttc ttaagctcac aaaagcacct cttaaaatag ttaagattcc
     8701 atctggtaat cattcattaa tcaagaacca cgagtccttt tatgaaatgg gaaaagctaa
     8761 tttcatgatt aaggagattg ataaatgaga gtacttatat cagctgtagg tgacacagac
     8821 ccttttagaa attttcatga tggctcctta attcatattg caagaaaata tcgtcccgaa
     8881 aaagttatat tgattttttc agaacatact gcaaaaaaac aaggtaatat tgaaaaagcc
     8941 ttgttttcta ttgctcctaa ttatgagcct gaactcataa ttcatgatcc cattattagt
     9001 gataatgagg ttcatatttt tgatgtgatg tttcaacgtt tctctgatat tttgcaagaa
     9061 tactatacta aagaagatga atttatcttg aatctttcta gcgcaacacc acaaatcaaa
     9121 tctgctctat ttgtgattaa tcgtttaaat ggtatcaatg tcaaagcggt tcaagtttcg
     9181 agtcccgaac acgcttcaaa cgagaatatc ggtcatgata atgatgagaa tatcgatgaa
     9241 ctcatcgaag tcaataaaga taataaagtc aatttcattg atcgaacgat tgaagataat
     9301 gccgaaaaat ttagtcaggc tctattgaag aaaactgctc gagattttat tgagaagttt
     9361 gattataagg cggcactaga tattctagac caattatcag atttcccgaa tttaaaaagt
     9421 gttagagaag aaatccgcga tgttgtcaac tgtctgtcta aacaagatgt tccaaaaggg
     9481 ttacgtcata aaaaattgaa ggaagaagaa caaaaaattc ttagtgccta tttgaccatt
     9541 gaactacaaa gagaacgtgg gaacgtaagt gaaagtttta tacgaataaa aaatttaacg
     9601 gaatttattt tggaagacta tattgaaaaa agatacccag gtttaataga tgaatattgt
     9661 gaagatattc agaaatatta tctaagtctc tttgattaca gtaaactttt aaaagcaacg
     9721 aaagaattta aacttaagag aacaatcgct ccaattattg atatgaattc ttctagaaat
     9781 aaggttgccc acagcttaag tcctttggac tcagatgcag ttaaacaact ggggatagct
     9841 atgaaaacct taaaaacttt ggttagagaa caataccatt ttagccagag tgattttaat
     9901 ttttatcatg atttgaataa aatattattg actaagttga actaaagagg tgatccaagt
     9961 tgaaaatatt aatctctgct gtggggacca ctgatcctat aagtaataat cacgatgcag
    10021 ctttacttca tatcgcaaga aattatagac ctgacaagat tgtccttgta tatagtcaag
    10081 aaatgatggt caaacaagat ttgataaata aggttttgct ctcaatcgaa ggctataacc
    10141 ctatcattga aattgactct acaatcttga ataacgacga agtctttctt tttgacaaaa
    10201 tgtatgaagt tatggggcaa attgttcaaa agtatactaa tgacgataac gaaatcatct
    10261 tgaacttgtc atcaggtaca ccacaaatta tttcagctct ctttgccctt aaccgtatca
    10321 atgactataa cacacaagct attcaggttg ctactcctaa aaatagagca aacagagaat
    10381 acacagctct gactgagtct gagatagatg ctctaattat ggaaaatcaa gataataggc
    10441 tggattttgt tgaccgaagt attaaagata aatctgagaa atttactcaa gcattagtca
    10501 aacgtcatct tagaagttta attgcttcct ttgattatca agcagcagaa gcgataatca
    10561 atcgaaaaga atataataaa ttactttcta aaaagaaaat cgcttatatt cgtgagaaac
    10621 tatacgattt ttcacgagtt ttcaaaaatc agtctattct ttcagatatc ctatcgtttc
    10681 ctctcgatga ctcccaaaag aaggctttga attactattt gatgattgat gttttaaaag
    10741 aacgtgagca tattgctgat gtcttaatta aggcaaaatc actagctgaa tttgtcatcg
    10801 aagaaaccat caaaaaagat catgagggct taattgtatt tgatggcaat ctgccaaaac
    10861 ttaatccaag ttttcctgat tgtgaggcaa ttttagacga tattgataag aaaatgaaaa
    10921 aatctagggg tattgaagac actgaagaac gcattttttc agtacaatct acacttaatc
    10981 tattatctta cttgaatatc cttgagttct atgaatatga ttctcagctt cagacagcga
    11041 ttaatggcat tttaagtttg aatggtgaac gaaataaagt tgctcacggt ttgtcagaaa
    11101 ttgatacgcg attattaagt cgtaagaagt taaaacaact ttctgaaaac ttgcgattgc
    11161 ttcttgttga ttgtttgggt attgattcga gctattttaa ctactatgac aaacaaaata
    11221 aagagcttat aaagatgctt gaatagatat ttgggtgtca tccccctttg acattcccta
    11281 ataaaagcgc tataatagaa caaattgaac tgaaccttta atcgagtcca gagaggcgag
    11341 aaaggtgtac tgagatgtta tggcatcact aagggtaggg actgccctgt tttcgtgcaa
    11401 ccttcgctga ctggcggagg tttttttgtg aaaggatata agaaatgagt gaaaaacgtc
    11461 ctgttattgc ccttgatttc ccgacttttg aggatgtgaa ggcttttctt gttaagtttc
    11521 ctgctgatga aaagctctat gtg
//