﻿               SEQUENCE LISTING

<110> Heinrich-Pette-Institut Leibniz-Institut für Exp. Virologie
      Technische Universität Dresden

<120> Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains

<130> HEI15463PCT

<160> 14

<170> BiSSAP 1.3

<210> 1
<211> 34
<212> DNA
<213> Human immunodeficiency virus 1

<400> 1
aacccactgc ttaagcctca ataaagcttg cctt                                 34


<210> 2
<211> 34
<212> DNA
<213> Human immunodeficiency virus 1

<400> 2
ctgggcggga ctggggagtg gcgagccctc agat                                 34


<210> 3
<211> 34
<212> DNA
<213> Human immunodeficiency virus 1

<400> 3
acaacatcct attacaccct atatgccaac atgg                                 34


<210> 4
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> loxP

<400> 4
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210> 5
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> loxH

<400> 5
atatatacgt atatagacat atatacgtat atat                                 34


<210> 6
<211> 343
<212> PRT
<213> Enterobacteria phage P1

<400> 6
Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      
Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  
Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         
Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 
Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     
Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         
Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

<210> 7
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tre common consensus seuqence 100% (WO 2011/147590)

<220> 
<221> VARIANT
<222> 3..5

<220> 
<221> VARIANT
<222> 7..10

<220> 
<221> VARIANT
<222> 12..12

<220> 
<221> VARIANT
<222> 15..16

<220> 
<221> VARIANT
<222> 18..19

<220> 
<221> VARIANT
<222> 22..26

<220> 
<221> VARIANT
<222> 28..31

<220> 
<221> VARIANT
<222> 34..35

<220> 
<221> VARIANT
<222> 37..37

<220> 
<221> VARIANT
<222> 39..40

<220> 
<221> VARIANT
<222> 43..44

<220> 
<221> VARIANT
<222> 48..48

<220> 
<221> VARIANT
<222> 51..51

<220> 
<221> VARIANT
<222> 53..53

<220> 
<221> VARIANT
<222> 57..60

<220> 
<221> VARIANT
<222> 62..63

<220> 
<221> VARIANT
<222> 66..67

<220> 
<221> VARIANT
<222> 69..70

<220> 
<221> VARIANT
<222> 73..73

<220> 
<221> VARIANT
<222> 77..77

<220> 
<221> VARIANT
<222> 80..80

<220> 
<221> VARIANT
<222> 84..84

<220> 
<221> VARIANT
<222> 86..86

<220> 
<221> VARIANT
<222> 88..88

<220> 
<221> VARIANT
<222> 93..94

<220> 
<221> VARIANT
<222> 98..98

<220> 
<221> VARIANT
<222> 102

<220> 
<221> VARIANT
<222> 105

<220> 
<221> VARIANT
<222> 107..108

<220> 
<221> VARIANT
<222> 110..112

<220> 
<221> VARIANT
<222> 116..117

<220> 
<221> VARIANT
<222> 122

<220> 
<221> VARIANT
<222> 131

<220> 
<221> VARIANT
<222> 138

<220> 
<221> VARIANT
<222> 140

<220> 
<221> VARIANT
<222> 142..147

<220> 
<221> VARIANT
<222> 149..151

<220> 
<221> VARIANT
<222> 153-156

<220> 
<221> VARIANT
<222> 158

<220> 
<221> VARIANT
<222> 160

<220> 
<221> VARIANT
<222> 163

<220> 
<221> VARIANT
<222> 166

<220> 
<221> VARIANT
<222> 174-175

<220> 
<221> VARIANT
<222> 177..180

<220> 
<221> VARIANT
<222> 182..183

<220> 
<221> VARIANT
<222> 185

<220> 
<221> VARIANT
<222> 187

<220> 
<221> VARIANT
<222> 189

<220> 
<221> VARIANT
<222> 193

<220> 
<221> VARIANT
<222> 197..199

<220> 
<221> VARIANT
<222> 207

<220> 
<221> VARIANT
<222> 216..217

<220> 
<221> VARIANT
<222> 219

<220> 
<221> VARIANT
<222> 221

<220> 
<221> VARIANT
<222> 225

<220> 
<221> VARIANT
<222> 227

<220> 
<221> VARIANT
<222> 232..236

<220> 
<221> VARIANT
<222> 241..245

<220> 
<221> VARIANT
<222> 247

<220> 
<221> VARIANT
<222> 249

<220> 
<221> VARIANT
<222> 251

<220> 
<221> VARIANT
<222> 253..255

<220> 
<221> VARIANT
<222> 258..260

<220> 
<221> VARIANT
<222> 262..263

<220> 
<221> VARIANT
<222> 266..268

<220> 
<221> VARIANT
<222> 270..273

<220> 
<221> VARIANT
<222> 277..278

<220> 
<221> VARIANT
<222> 281

<220> 
<221> VARIANT
<222> 284..285

<220> 
<221> VARIANT
<222> 305

<220> 
<221> VARIANT
<222> 307

<220> 
<221> VARIANT
<222> 316

<220> 
<221> VARIANT
<222> 319..320

<220> 
<221> VARIANT
<222> 332

<220> 
<221> VARIANT
<222> 341..343

<400> 7
Met Ser Xaa Xaa Xaa Thr Xaa Xaa Xaa Xaa Leu Xaa Ala Leu Xaa Xaa 
1               5                   10                  15      
Asp Xaa Xaa Ser Asp Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Xaa Arg 
            20                  25                  30          
Asp Xaa Xaa Ala Xaa Ser Xaa Xaa Thr Trp Xaa Xaa Leu Leu Ser Xaa 
        35                  40                  45              
Cys Arg Xaa Trp Xaa Ala Trp Cys Xaa Xaa Xaa Xaa Arg Xaa Xaa Phe 
    50                  55                  60                  
Pro Xaa Xaa Pro Xaa Xaa Val Arg Xaa Tyr Leu Leu Xaa Leu Gln Xaa 
65                  70                  75                  80  
Arg Gly Leu Xaa Val Xaa Thr Xaa Gln Gln His Leu Xaa Xaa Leu Asn 
                85                  90                  95      
Met Xaa His Arg Arg Xaa Gly Leu Xaa Arg Xaa Xaa Asp Xaa Xaa Xaa 
            100                 105                 110         
Val Ser Leu Xaa Xaa Arg Arg Ile Arg Xaa Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Xaa Lys Gln Ala Leu Ala Phe Xaa Arg Xaa Asp Xaa Xaa Xaa 
    130                 135                 140                 
Xaa Xaa Xaa Leu Xaa Xaa Xaa Ser Xaa Xaa Xaa Xaa Asp Xaa Arg Xaa 
145                 150                 155                 160 
Leu Ala Xaa Leu Gly Xaa Ala Tyr Asn Thr Leu Leu Arg Xaa Xaa Glu 
                165                 170                 175     
Xaa Xaa Xaa Xaa Arg Xaa Xaa Asp Xaa Ser Xaa Thr Xaa Gly Gly Arg 
            180                 185                 190         
Xaa Leu Ile His Xaa Xaa Xaa Thr Lys Thr Leu Val Ser Thr Xaa Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Xaa Xaa Thr Xaa Leu Xaa Glu Arg Trp 
    210                 215                 220                 
Xaa Ser Xaa Ser Gly Val Ala Xaa Xaa Xaa Xaa Xaa Tyr Leu Phe Cys 
225                 230                 235                 240 
Xaa Xaa Xaa Xaa Xaa Gly Xaa Ala Xaa Pro Xaa Ala Xaa Xaa Xaa Leu 
                245                 250                 255     
Ser Xaa Xaa Xaa Leu Xaa Xaa Ile Phe Xaa Xaa Xaa His Xaa Xaa Xaa 
            260                 265                 270         
Xaa Gly Ala Lys Xaa Xaa Ser Gly Xaa Arg Tyr Xaa Xaa Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Xaa Ile Xaa Glu Ile Met Gln Ala Gly Gly Trp Xaa Thr Val Xaa Xaa 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Xaa Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Xaa Xaa Xaa 
            340             

<210> 8
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tre 3.0 consensus sequence 100% (WO 2011/147590)

<220> 
<221> VARIANT
<222> 3..5

<220> 
<221> VARIANT
<222> 7..10

<220> 
<221> VARIANT
<222> 16

<220> 
<221> VARIANT
<222> 18..19

<220> 
<221> VARIANT
<222> 22..26

<220> 
<221> VARIANT
<222> 28..31

<220> 
<221> VARIANT
<222> 34..35

<220> 
<221> VARIANT
<222> 37

<220> 
<221> VARIANT
<222> 39..40

<220> 
<221> VARIANT
<222> 43

<220> 
<221> VARIANT
<222> 48

<220> 
<221> VARIANT
<222> 51

<220> 
<221> VARIANT
<222> 53

<220> 
<221> VARIANT
<222> 57..60

<220> 
<221> VARIANT
<222> 62..63

<220> 
<221> VARIANT
<222> 66..67

<220> 
<221> VARIANT
<222> 69..70

<220> 
<221> VARIANT
<222> 73

<220> 
<221> VARIANT
<222> 77

<220> 
<221> VARIANT
<222> 80

<220> 
<221> VARIANT
<222> 84

<220> 
<221> VARIANT
<222> 88

<220> 
<221> VARIANT
<222> 94

<220> 
<221> VARIANT
<222> 98

<220> 
<221> VARIANT
<222> 102

<220> 
<221> VARIANT
<222> 105

<220> 
<221> VARIANT
<222> 107..108

<220> 
<221> VARIANT
<222> 111..112

<220> 
<221> VARIANT
<222> 116..117

<220> 
<221> VARIANT
<222> 122

<220> 
<221> VARIANT
<222> 131

<220> 
<221> VARIANT
<222> 138

<220> 
<221> VARIANT
<222> 140

<220> 
<221> VARIANT
<222> 142..147

<220> 
<221> VARIANT
<222> 149..151

<220> 
<221> VARIANT
<222> 153..156

<220> 
<221> VARIANT
<222> 158

<220> 
<221> VARIANT
<222> 160

<220> 
<221> VARIANT
<222> 163

<220> 
<221> VARIANT
<222> 166

<220> 
<221> VARIANT
<222> 174

<220> 
<221> VARIANT
<222> 177..180

<220> 
<221> VARIANT
<222> 182..183

<220> 
<221> VARIANT
<222> 185

<220> 
<221> VARIANT
<222> 187

<220> 
<221> VARIANT
<222> 189

<220> 
<221> VARIANT
<222> 193

<220> 
<221> VARIANT
<222> 197..199

<220> 
<221> VARIANT
<222> 207

<220> 
<221> VARIANT
<222> 216..217

<220> 
<221> VARIANT
<222> 219

<220> 
<221> VARIANT
<222> 221

<220> 
<221> VARIANT
<222> 225

<220> 
<221> VARIANT
<222> 227

<220> 
<221> VARIANT
<222> 232..236

<220> 
<221> VARIANT
<222> 241..245

<220> 
<221> VARIANT
<222> 247

<220> 
<221> VARIANT
<222> 249

<220> 
<221> VARIANT
<222> 251

<220> 
<221> VARIANT
<222> 253..255

<220> 
<221> VARIANT
<222> 258..260

<220> 
<221> VARIANT
<222> 262..263

<220> 
<221> VARIANT
<222> 266..268

<220> 
<221> VARIANT
<222> 270..273

<220> 
<221> VARIANT
<222> 277..278

<220> 
<221> VARIANT
<222> 281

<220> 
<221> VARIANT
<222> 284..285

<220> 
<221> VARIANT
<222> 305

<220> 
<221> VARIANT
<222> 316

<220> 
<221> VARIANT
<222> 319..320

<220> 
<221> VARIANT
<222> 332

<220> 
<221> VARIANT
<222> 341..343

<400> 8
Met Ser Xaa Xaa Xaa Thr Xaa Xaa Xaa Xaa Leu Ser Ala Leu Leu Xaa 
1               5                   10                  15      
Asp Xaa Xaa Ser Asp Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Xaa Arg 
            20                  25                  30          
Asp Xaa Xaa Ala Xaa Ser Xaa Xaa Thr Trp Xaa Val Leu Leu Ser Xaa 
        35                  40                  45              
Cys Arg Xaa Trp Xaa Ala Trp Cys Xaa Xaa Xaa Xaa Arg Xaa Xaa Phe 
    50                  55                  60                  
Pro Xaa Xaa Pro Xaa Xaa Val Arg Xaa Tyr Leu Leu Xaa Leu Gln Xaa 
65                  70                  75                  80  
Arg Gly Leu Xaa Val Asn Thr Xaa Gln Gln His Leu Ala Xaa Leu Asn 
                85                  90                  95      
Met Xaa His Arg Arg Xaa Gly Leu Xaa Arg Xaa Xaa Asp Ser Xaa Xaa 
            100                 105                 110         
Val Ser Leu Xaa Xaa Arg Arg Ile Arg Xaa Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Xaa Lys Gln Ala Leu Ala Phe Xaa Arg Xaa Asp Xaa Xaa Xaa 
    130                 135                 140                 
Xaa Xaa Xaa Leu Xaa Xaa Xaa Ser Xaa Xaa Xaa Xaa Asp Xaa Arg Xaa 
145                 150                 155                 160 
Leu Ala Xaa Leu Gly Xaa Ala Tyr Asn Thr Leu Leu Arg Xaa Ser Glu 
                165                 170                 175     
Xaa Xaa Xaa Xaa Arg Xaa Xaa Asp Xaa Ser Xaa Thr Xaa Gly Gly Arg 
            180                 185                 190         
Xaa Leu Ile His Xaa Xaa Xaa Thr Lys Thr Leu Val Ser Thr Xaa Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Xaa Xaa Thr Xaa Leu Xaa Glu Arg Trp 
    210                 215                 220                 
Xaa Ser Xaa Ser Gly Val Ala Xaa Xaa Xaa Xaa Xaa Tyr Leu Phe Cys 
225                 230                 235                 240 
Xaa Xaa Xaa Xaa Xaa Gly Xaa Ala Xaa Pro Xaa Ala Xaa Xaa Xaa Leu 
                245                 250                 255     
Ser Xaa Xaa Xaa Leu Xaa Xaa Ile Phe Xaa Xaa Xaa His Xaa Xaa Xaa 
            260                 265                 270         
Xaa Gly Ala Lys Xaa Xaa Ser Gly Xaa Arg Tyr Xaa Xaa Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Xaa Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Xaa Thr Val Xaa Xaa 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Xaa Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Xaa Xaa Xaa 
            340             

<210> 9
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tre 3.1 consensus sequence 85% (all clones)

<220> 
<221> VARIANT
<222> 3..5

<220> 
<221> VARIANT
<222> 8..10

<220> 
<221> VARIANT
<222> 16

<220> 
<221> VARIANT
<222> 18..19

<220> 
<221> VARIANT
<222> 22..26

<220> 
<221> VARIANT
<222> 28..29

<220> 
<221> VARIANT
<222> 31

<220> 
<221> VARIANT
<222> 34..35

<220> 
<221> VARIANT
<222> 37

<220> 
<221> VARIANT
<222> 39

<220> 
<221> VARIANT
<222> 43

<220> 
<221> VARIANT
<222> 48

<220> 
<221> VARIANT
<222> 53

<220> 
<221> VARIANT
<222> 57..60

<220> 
<221> VARIANT
<222> 62..63

<220> 
<221> VARIANT
<222> 66..67

<220> 
<221> VARIANT
<222> 69..70

<220> 
<221> VARIANT
<222> 73

<220> 
<221> VARIANT
<222> 80

<220> 
<221> VARIANT
<222> 84

<220> 
<221> VARIANT
<222> 88

<220> 
<221> VARIANT
<222> 94

<220> 
<221> VARIANT
<222> 98

<220> 
<221> VARIANT
<222> 102

<220> 
<221> VARIANT
<222> 105

<220> 
<221> VARIANT
<222> 107

<220> 
<221> VARIANT
<222> 111..112

<220> 
<221> VARIANT
<222> 116..117

<220> 
<221> VARIANT
<222> 122

<220> 
<221> VARIANT
<222> 131

<220> 
<221> VARIANT
<222> 138

<220> 
<221> VARIANT
<222> 140

<220> 
<221> VARIANT
<222> 142..147

<220> 
<221> VARIANT
<222> 149..151

<220> 
<221> VARIANT
<222> 153..156

<220> 
<221> VARIANT
<222> 158

<220> 
<221> VARIANT
<222> 163

<220> 
<221> VARIANT
<222> 166

<220> 
<221> VARIANT
<222> 174

<220> 
<221> VARIANT
<222> 177..180

<220> 
<221> VARIANT
<222> 182..183

<220> 
<221> VARIANT
<222> 185

<220> 
<221> VARIANT
<222> 187

<220> 
<221> VARIANT
<222> 189

<220> 
<221> VARIANT
<222> 193

<220> 
<221> VARIANT
<222> 197..199

<220> 
<221> VARIANT
<222> 207

<220> 
<221> VARIANT
<222> 216..217

<220> 
<221> VARIANT
<222> 219

<220> 
<221> VARIANT
<222> 221

<220> 
<221> VARIANT
<222> 225

<220> 
<221> VARIANT
<222> 227

<220> 
<221> VARIANT
<222> 232..236

<220> 
<221> VARIANT
<222> 242..243

<220> 
<221> VARIANT
<222> 245

<220> 
<221> VARIANT
<222> 247

<220> 
<221> VARIANT
<222> 249

<220> 
<221> VARIANT
<222> 251

<220> 
<221> VARIANT
<222> 253..255

<220> 
<221> VARIANT
<222> 258

<220> 
<221> VARIANT
<222> 260

<220> 
<221> VARIANT
<222> 263

<220> 
<221> VARIANT
<222> 266..267

<220> 
<221> VARIANT
<222> 270..273

<220> 
<221> VARIANT
<222> 277

<220> 
<221> VARIANT
<222> 281

<220> 
<221> VARIANT
<222> 284..285

<220> 
<221> VARIANT
<222> 305

<220> 
<221> VARIANT
<222> 316

<220> 
<221> VARIANT
<222> 332

<220> 
<221> VARIANT
<222> 341..343

<400> 9
Met Ser Xaa Xaa Xaa Thr Leu Xaa Xaa Xaa Leu Ser Ala Leu Leu Xaa 
1               5                   10                  15      
Asp Xaa Xaa Ser Asp Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Val Xaa Arg 
            20                  25                  30          
Asp Xaa Xaa Ala Xaa Ser Xaa Arg Thr Trp Xaa Val Leu Leu Ser Xaa 
        35                  40                  45              
Cys Arg Thr Trp Xaa Ala Trp Cys Xaa Xaa Xaa Xaa Arg Xaa Xaa Phe 
    50                  55                  60                  
Pro Xaa Xaa Pro Xaa Xaa Val Arg Xaa Tyr Leu Leu His Leu Gln Xaa 
65                  70                  75                  80  
Arg Gly Leu Xaa Val Asn Thr Xaa Leu Gln His Leu Ala Xaa Leu Asn 
                85                  90                  95      
Met Xaa His Arg Arg Xaa Gly Leu Xaa Arg Xaa Gly Asp Ser Xaa Xaa 
            100                 105                 110         
Val Ser Leu Xaa Xaa Arg Arg Ile Arg Xaa Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Xaa Lys Gln Ala Leu Ala Phe Xaa Arg Xaa Asp Xaa Xaa Xaa 
    130                 135                 140                 
Xaa Xaa Xaa Leu Xaa Xaa Xaa Ser Xaa Xaa Gly Xaa Asp Xaa Arg Thr 
145                 150                 155                 160 
Leu Ala Xaa Leu Gly Xaa Ala Tyr Asn Thr Leu Leu Arg Xaa Ser Glu 
                165                 170                 175     
Xaa Xaa Xaa Xaa Arg Xaa Xaa Asp Xaa Ser Xaa Thr Xaa Gly Gly Arg 
            180                 185                 190         
Xaa Leu Ile His Xaa Xaa Xaa Thr Lys Thr Leu Val Ser Thr Xaa Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Xaa Xaa Thr Xaa Leu Xaa Glu Arg Trp 
    210                 215                 220                 
Xaa Ser Xaa Ser Gly Val Ala Xaa Xaa Xaa Xaa Xaa Tyr Leu Phe Cys 
225                 230                 235                 240 
Gln Xaa Xaa Ile Xaa Gly Xaa Ala Val Pro Xaa Ala Xaa Xaa Xaa Leu 
                245                 250                 255     
Ser Xaa Asp Xaa Leu Arg Xaa Ile Phe Xaa Xaa Ala His Xaa Xaa Xaa 
            260                 265                 270         
Xaa Gly Ala Lys Xaa Gly Ser Gly Xaa Arg Tyr Xaa Xaa Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Xaa Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Xaa Thr Val Glu Ser 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Xaa Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Xaa Xaa Xaa 
            340             

<210> 10
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> Tre 3.1 consensus 100% (3 clones)

<220> 
<221> VARIANT
<222> 3

<220> 
<221> VARIANT
<222> 5

<220> 
<221> VARIANT
<222> 10

<220> 
<221> VARIANT
<222> 18..19

<220> 
<221> VARIANT
<222> 23

<220> 
<221> VARIANT
<222> 57

<220> 
<221> VARIANT
<222> 63

<220> 
<221> VARIANT
<222> 80

<220> 
<221> VARIANT
<222> 84

<220> 
<221> VARIANT
<222> 88

<220> 
<221> VARIANT
<222> 111

<220> 
<221> VARIANT
<222> 117

<220> 
<221> VARIANT
<222> 151

<220> 
<221> VARIANT
<222> 153

<220> 
<221> VARIANT
<222> 160

<220> 
<221> VARIANT
<222> 163

<220> 
<221> VARIANT
<222> 174

<220> 
<221> VARIANT
<222> 182..183

<220> 
<221> VARIANT
<222> 198

<220> 
<221> VARIANT
<222> 219

<220> 
<221> VARIANT
<222> 232

<220> 
<221> VARIANT
<222> 241

<220> 
<221> VARIANT
<222> 244

<220> 
<221> VARIANT
<222> 247

<220> 
<221> VARIANT
<222> 255

<220> 
<221> VARIANT
<222> 263

<220> 
<221> VARIANT
<222> 266

<220> 
<221> VARIANT
<222> 272

<220> 
<221> VARIANT
<222> 319

<400> 10
Met Ser Xaa Leu Xaa Thr Leu His Gln Xaa Leu Ser Ala Leu Leu Val 
1               5                   10                  15      
Asp Xaa Xaa Ser Asp Glu Xaa Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu Arg Thr Trp Lys Val Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Thr Trp Ala Ala Trp Cys Xaa Leu Asn Asn Arg Lys Xaa Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Xaa 
65                  70                  75                  80  
Arg Gly Leu Xaa Val Asn Thr Xaa Leu Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Phe Gly Leu Pro Arg Pro Gly Asp Ser Xaa Ala 
            100                 105                 110         
Val Ser Leu Val Xaa Arg Arg Ile Arg Arg Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Thr Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ala Leu Met Glu Xaa Ser Xaa Arg Gly Gln Asp Ile Arg Xaa 
145                 150                 155                 160 
Leu Ala Xaa Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Xaa Ser Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Xaa Xaa Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Xaa Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Xaa Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Xaa Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Xaa Val Arg Xaa Asn Gly Xaa Ala Val Pro Ser Ala Thr Ser Xaa Leu 
                245                 250                 255     
Ser Thr Asp Val Leu Arg Xaa Ile Phe Xaa Ala Ala His Arg Leu Xaa 
            260                 265                 270         
Tyr Gly Ala Lys Asp Gly Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Xaa Ser 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

<210> 11
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> uTre

<400> 11
Met Ser Ile Leu Leu Thr Leu His Gln Ser Leu Ser Ala Leu Leu Val 
1               5                   10                  15      
Asp Ala Thr Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu Arg Thr Trp Lys Val Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Thr Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Ala 
65                  70                  75                  80  
Arg Gly Leu Ala Val Asn Thr Ile Leu Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Phe Gly Leu Pro Arg Pro Gly Asp Ser Asp Ala 
            100                 105                 110         
Val Ser Leu Val Met Arg Arg Ile Arg Arg Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Thr Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ala Leu Met Glu Asn Ser Glu Arg Gly Gln Asp Ile Arg Thr 
145                 150                 155                 160 
Leu Ala Leu Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Val Ser Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Ile Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Ser Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Ser Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Gln Val Arg Ile Asn Gly Val Ala Val Pro Ser Ala Thr Ser Arg Leu 
                245                 250                 255     
Ser Thr Asp Val Leu Arg Lys Ile Phe Glu Ala Ala His Arg Leu Ile 
            260                 265                 270         
Tyr Gly Ala Lys Asp Gly Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

<210> 12
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> tailored recombinase

<400> 12
Met Ser Ser Leu Gln Thr Leu His Gln Asn Leu Ser Ala Leu Leu Val 
1               5                   10                  15      
Asp Val Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu Arg Thr Trp Lys Val Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Thr Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Arg Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Thr 
65                  70                  75                  80  
Arg Gly Leu Thr Val Asn Thr Ile Leu Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Phe Gly Leu Pro Arg Pro Gly Asp Ser Asp Ala 
            100                 105                 110         
Val Ser Leu Val Ile Arg Arg Ile Arg Arg Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Thr Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ala Leu Met Glu Asn Ser Asp Arg Gly Gln Asp Ile Arg Ser 
145                 150                 155                 160 
Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Val Arg Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Ser Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Pro Val Arg Val Asn Gly Ala Ala Val Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     
Ser Thr Asp Val Leu Arg Gly Ile Phe Glu Ala Ala His Arg Leu Val 
            260                 265                 270         
Tyr Gly Ala Lys Asp Gly Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Glu Ser 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

<210> 13
<211> 343
<212> PRT
<213> Artificial Sequence

<220> 
<223> tailored recombinase

<400> 13
Met Ser Ser Leu Leu Thr Leu His Gln Ser Leu Ser Ala Leu Leu Val 
1               5                   10                  15      
Asp Val Ala Ser Asp Glu Ala Arg Lys Asn Leu Met Asp Val Leu Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu Arg Thr Trp Lys Val Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Thr Trp Ala Ala Trp Cys Glu Leu Asn Asn Arg Lys Arg Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu His Leu Gln Thr 
65                  70                  75                  80  
Arg Gly Leu Thr Val Asn Thr Val Leu Gln His Leu Ala Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Phe Gly Leu Pro Arg Pro Gly Asp Ser Asn Ala 
            100                 105                 110         
Val Ser Leu Val Ile Arg Arg Ile Arg Arg Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Thr Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ala Leu Met Glu Asp Ser Asp Arg Gly Gln Asp Ile Arg Ser 
145                 150                 155                 160 
Leu Ala Phe Leu Gly Val Ala Tyr Asn Thr Leu Leu Arg Ile Ser Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Val Arg Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Glu Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Gln Val Arg Ile Asn Gly Val Ala Val Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     
Ser Thr Asp Val Leu Arg Gly Ile Phe Ala Ala Ala His Arg Leu Ile 
            260                 265                 270         
Tyr Gly Ala Lys Asp Gly Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Ala Glu Ile Met Gln Ala Gly Gly Trp Thr Thr Val Asp Ser 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Ser Asp 
            340             

<210> 14
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> uTre with nuclear localization signal

<220> 
<221> SIGNAL
<222> 2..9
<223> nuclear localisation sequence (NLS)

<400> 14
Met Val Pro Lys Lys Lys Arg Lys Val Ser Ile Leu Leu Thr Leu His 
1               5                   10                  15      
Gln Ser Leu Ser Ala Leu Leu Val Asp Ala Thr Ser Asp Glu Ala Arg 
            20                  25                  30          
Lys Asn Leu Met Asp Val Leu Arg Asp Arg Gln Ala Phe Ser Glu Arg 
        35                  40                  45              
Thr Trp Lys Val Leu Leu Ser Val Cys Arg Thr Trp Ala Ala Trp Cys 
    50                  55                  60                  
Lys Leu Asn Asn Arg Lys Trp Phe Pro Ala Glu Pro Glu Asp Val Arg 
65                  70                  75                  80  
Asp Tyr Leu Leu His Leu Gln Ala Arg Gly Leu Ala Val Asn Thr Ile 
                85                  90                  95      
Leu Gln His Leu Ala Gln Leu Asn Met Leu His Arg Arg Phe Gly Leu 
            100                 105                 110         
Pro Arg Pro Gly Asp Ser Asp Ala Val Ser Leu Val Met Arg Arg Ile 
        115                 120                 125             
Arg Arg Glu Asn Val Asp Ala Gly Glu Arg Thr Lys Gln Ala Leu Ala 
    130                 135                 140                 
Phe Glu Arg Thr Asp Phe Asp Gln Val Arg Ala Leu Met Glu Asn Ser 
145                 150                 155                 160 
Glu Arg Gly Gln Asp Ile Arg Thr Leu Ala Leu Leu Gly Val Ala Tyr 
                165                 170                 175     
Asn Thr Leu Leu Arg Val Ser Glu Ile Ala Arg Ile Arg Ile Lys Asp 
            180                 185                 190         
Ile Ser Arg Thr Asp Gly Gly Arg Met Leu Ile His Ile Ser Arg Thr 
        195                 200                 205             
Lys Thr Leu Val Ser Thr Ala Gly Val Glu Lys Ala Leu Ser Leu Gly 
    210                 215                 220                 
Val Thr Lys Leu Val Glu Arg Trp Ile Ser Val Ser Gly Val Ala Ser 
225                 230                 235                 240 
Asp Pro Asn Asn Tyr Leu Phe Cys Gln Val Arg Ile Asn Gly Val Ala 
                245                 250                 255     
Val Pro Ser Ala Thr Ser Arg Leu Ser Thr Asp Val Leu Arg Lys Ile 
            260                 265                 270         
Phe Glu Ala Ala His Arg Leu Ile Tyr Gly Ala Lys Asp Gly Ser Gly 
        275                 280                 285             
Gln Arg Tyr Leu Ala Trp Ser Gly His Ser Ala Arg Val Gly Ala Ala 
    290                 295                 300                 
Arg Asp Met Ala Arg Ala Gly Val Ser Ile Ala Glu Ile Met Gln Ala 
305                 310                 315                 320 
Gly Gly Trp Thr Thr Val Glu Ser Val Met Asn Tyr Ile Arg Asn Leu 
                325                 330                 335     
Asp Ser Glu Thr Gly Ala Met Val Arg Leu Leu Glu Asp Gly Asp 
            340                 345                 350     

