                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Modified microorganism for improved production of fine chemicals 
       on sucrose

<130>  INV77019

<150>  EP 14182674.3
<151>  2014-08-28

<160>  29    

<170>  PatentIn version 3.3

<210>  1
<211>  1517
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1517)
<223>  16 S rDNA of strain DD1

<400>  1
tttgatcctg gctcagattg aacgctggcg gcaggcttaa cacatgcaag tcgaacggta     60

gcgggaggaa agcttgcttt ctttgccgac gagtggcgga cgggtgagta atgcttgggg    120

atctggctta tggaggggga taacgacggg aaactgtcgc taataccgcg taatatcttc    180

ggattaaagg gtgggacttt cgggccaccc gccataagat gagcccaagt gggattaggt    240

agttggtggg gtaaaggcct accaagccga cgatctctag ctggtctgag aggatgacca    300

gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg    360

cacaatgggg ggaaccctga tgcagccatg ccgcgtgaat gaagaaggcc ttcgggttgt    420

aaagttcttt cggtgacgag gaaggtgttt gttttaatag gacaagcaat tgacgttaat    480

cacagaagaa gcaccggcta actccgtgcc agcagccgcg gtaatacgga gggtgcgagc    540

gttaatcgga ataactgggc gtaaagggca tgcaggcgga cttttaagtg agatgtgaaa    600

gccccgggct taacctggga attgcatttc agactgggag tctagagtac tttagggagg    660

ggtagaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaatac cgaaggcgaa    720

ggcagcccct tgggaagata ctgacgctca tatgcgaaag cgtggggagc aaacaggatt    780

agataccctg gtagtccacg cggtaaacgc tgtcgatttg gggattgggc tttaggcctg    840

gtgctcgtag ctaacgtgat aaatcgaccg cctggggagt acggccgcaa ggttaaaact    900

caaatgaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg    960

cgaagaacct tacctactct tgacatccag agaatcctgt agagatacgg gagtgccttc   1020

gggagctctg agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt   1080

aagtcccgca acgagcgcaa cccttatcct ttgttgccag catgtaaaga tgggaactca   1140

aaggagactg ccggtgacaa accggaggaa ggtggggatg acgtcaagtc atcatggccc   1200

ttacgagtag ggctacacac gtgctacaat ggtgcataca gagggcggcg ataccgcgag   1260

gtagagcgaa tctcagaaag tgcatcgtag tccggattgg agtctgcaac tcgactccat   1320

gaagtcggaa tcgctagtaa tcgcaaatca gaatgttgcg gtgaatacgt tcccgggcct   1380

tgtacacacc gcccgtcaca ccatgggagt gggttgtacc agaagtagat agcttaacct   1440

tcgggggggg cgtttaccac ggtatgattc atgactgggg tgaagtcgta acaaggtaac   1500

cgtaggggaa cctgcgg                                                  1517


<210>  2
<211>  3008
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(3008)
<223>  23 S rDNA of strain DD1

<400>  2
agtaataacg aacgacacag gtataagaat acttgaggtt gtatggttaa gtgactaagc     60

gtacaaggtg gatgccttgg caatcagagg cgaagaagga cgtgctaatc tgcgaaaagc    120

ttgggtgagt tgataagaag cgtctaaccc aagatatccg aatggggcaa cccagtagat    180

gaagaatcta ctatcaataa ccgaatccat aggttattga ggcaaaccgg gagaactgaa    240

acatctaagt accccgagga aaagaaatca accgagatta cgtcagtagc ggcgagcgaa    300

agcgtaagag ccggcaagtg atagcatgag gattagagga atcggctggg aagccgggcg    360

gcacagggtg atagccccgt acttgaaaat cattgtgtgg tactgagctt gcgagaagta    420

gggcgggaca cgagaaatcc tgtttgaaga aggggggacc atcctccaag gctaaatact    480

cctgattgac cgatagtgaa ccagtactgt gaaggaaagg cgaaaagaac cccggtgagg    540

ggagtgaaat agaacctgaa accttgtacg tacaagcagt gggagcccgc gagggtgact    600

gcgtaccttt tgtataatgg gtcagcgact tatattatgt agcgaggtta accgaatagg    660

ggagccgaag ggaaaccgag tcttaactgg gcgtcgagtt gcatgatata gacccgaaac    720

ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg    780

actaatgttg aaaaattagc ggatgacctg tggctggggg tgaaaggcca atcaaaccgg    840

gagatagctg gttctccccg aaatctattt aggtagagcc ttatgtgaat accttcgggg    900

gtagagcact gtttcggcta gggggccatc ccggcttacc aacccgatgc aaactgcgaa    960

taccgaagag taatgcatag gagacacacg gcgggtgcta acgttcgtcg tggagaggga   1020

aacaacccag accgccagct aaggtcccaa agtttatatt aagtgggaaa cgaagtggga   1080

aggcttagac agctaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag   1140

ctcactagtc gagtcggcct gcgcggaaga tgtaacgggg ctcaaatata gcaccgaagc   1200

tgcggcatca ggcgtaagcc tgttgggtag gggagcgtcg tgtaagcgga agaaggtggt   1260

tcgagagggc tgctggacgt atcacgagtg cgaatgctga cataagtaac gataaaacgg   1320

gtgaaaaacc cgttcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt   1380

gagtcggccc ctaaggcgag gctgaagagc gtagtcgatg ggaaacgggt taatattccc   1440

gtacttgtta taattgcgat gtggggacgg agtaggttag gttatcgacc tgttggaaaa   1500

ggtcgtttaa gttggtaggt ggagcgttta ggcaaatccg gacgcttatc aacaccgaga   1560

gatgatgacg aggcgctaag gtgccgaagt aaccgatacc acacttccag gaaaagccac   1620

taagcgtcag attataataa accgtactat aaaccgacac aggtggtcag gtagagaata   1680

ctcaggcgct tgagagaact cgggtgaagg aactaggcaa aatagcaccg taacttcggg   1740

agaaggtgcg ccggcgtaga ttgtagaggt atacccttga aggttgaacc ggtcgaagtg   1800

acccgctggc tgcaactgtt tattaaaaac acagcactct gcaaacacga aagtggacgt   1860

atagggtgtg atgcctgccc ggtgctggaa ggttaattga tggcgttatc gcaagagaag   1920

cgcctgatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa   1980

ttccttgtcg ggtaagttcc gacctgcacg aatggcataa tgatggccag gctgtctcca   2040

cccgagactc agtgaaattg aaatcgccgt gaagatgcgg tgtacccgcg gctagacgga   2100

aagaccccgt gaacctttac tatagcttga cactgaacct tgaattttga tgtgtaggat   2160

aggtgggagg ctttgaagcg gtaacgccag ttatcgtgga gccatccttg aaataccacc   2220

ctttaacgtt tgatgttcta acgaagtgcc cggaacgggt actcggacag tgtctggtgg   2280

gtagtttgac tggggcggtc tcctcccaaa gagtaacgga ggagcacgaa ggtttgctaa   2340

tgacggtcgg acatcgtcag gttagtgcaa tggtataagc aagcttaact gcgagacgga   2400

caagtcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca   2460

tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata   2520

tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc   2580

ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttaa aacgtcgtga   2640

gacagtttgg tccctatctg ccgtgggcgt tggagaattg agaggggctg ctcctagtac   2700

gagaggaccg gagtggacgc atcactggtg ttccggttgt gtcgccagac gcattgccgg   2760

gtagctacat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgcctcgaga   2820

tgagttctcc cagtatttaa tactgtaagg gttgttggag acgacgacgt agataggccg   2880

ggtgtgtaag cgttgcgaga cgttgagcta accggtacta attgcccgag aggcttagcc   2940

atacaacgct caagtgtttt tggtagtgaa agttattacg gaataagtaa gtagtcaggg   3000

aatcggct                                                            3008


<210>  3
<211>  1479
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1479)
<223>  nucleotide sequence of ptsG-gene from strain DD1

<400>  3
ttgctcgttt tagctagaat tggcgaaaat ttttgcttaa tttataaacg aggagtcgct     60

atgaactacc ctaaaattgc ccaacaggtt attgaaaaac ttggcgggaa agaaaatatc    120

gccaatcttg cgcattgtgc aacgcgtttg cgcttgacaa tgaatgacga aagtaaaatc    180

gacaaacagg ccattgaaga tattgagggc gtaaaagggc agttttcaac ctccggtcaa    240

taccaaatta ttttcggttc aggtacggtg aataaagttt acgccgaaat gaataccatt    300

atgaacggtt caccgtcggc ggattccacc ggggaaagtc aacaggcgaa agggccgcag    360

caaggtttga ttcaacgatt aattaaaggt ctggctgata ttttcgttcc cattattccg    420

gctattgtcg ccggcggttt gttaatgggg attaataatg tctttaccgc aaaagattta    480

ttcgaagaag ggaagacatt actcgacctt tatccgcaat acaaagattt agcggattta    540

attaatacct ttgctaacgc gccttttgtg tttctgcccg tattgttagg tttttcggca    600

accagaaaat tcggtggcaa tccgttctta ggagcgacat taggtatgtt gctcgttcac    660

cccgctttaa ccaatgctta cggttatgcg gaagcgttag ccggcggcaa tcttcaatta    720

tggaatattt tcgggctaga gattgaaaaa gtcggctatc aaggcacggt tattcccgtt    780

ttaattgccg cctgggtatt ggcgacttta gaaaaattct tagtgaaagt agtgccttcc    840

gtattaaata atttagtcac gccgttattt tcattattta tcaccggttt tttggctttc    900

accgtaatcg gacctttcgg tcgtgaagcg ggggaatttt taagtcaggg tttaacctgg    960

ttatatgata ctttaggttt tatcggcggc ggcgtgttcg gcgcattata cgcacctatc   1020

gtgattaccg gtatgcacca aacctttatc gccattgaaa cgcaattgct ggcaagcact   1080

gcggcaactt ttatcttccc gattgccgcc atgtcgaata ttgcgcaggg tgccgcttgt   1140

ttagccgttg ccgtgttaaa taaagatgcc aaaacccgag gtctggcgtt gccttccggc   1200

atttccgcat tattagggat taccgaacct gccatgttcg gggtgaattt gcgcttccgt   1260

tatccgttct atgcggctat gttaggtgcc ggttccgccg cggcgtttat tgcgttcttc   1320

aatgttaaag ccactgcgct tggcgcggcg ggcttaatcg gtatcgcatc aattcgtgcc   1380

ggcgactggg gaatgtattc cgtggggatg gtaatttcgt tttgtgtggc tttcgctgcg   1440

gcattagtgc tgggcgcaag agctaacgca aaagaatag                          1479


<210>  4
<211>  492
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(492)
<223>  amino acid sequence of PtsG from strain DD1

<400>  4

Met Leu Val Leu Ala Arg Ile Gly Glu Asn Phe Cys Leu Ile Tyr Lys 
1               5                   10                  15      


Arg Gly Val Ala Met Asn Tyr Pro Lys Ile Ala Gln Gln Val Ile Glu 
            20                  25                  30          


Lys Leu Gly Gly Lys Glu Asn Ile Ala Asn Leu Ala His Cys Ala Thr 
        35                  40                  45              


Arg Leu Arg Leu Thr Met Asn Asp Glu Ser Lys Ile Asp Lys Gln Ala 
    50                  55                  60                  


Ile Glu Asp Ile Glu Gly Val Lys Gly Gln Phe Ser Thr Ser Gly Gln 
65                  70                  75                  80  


Tyr Gln Ile Ile Phe Gly Ser Gly Thr Val Asn Lys Val Tyr Ala Glu 
                85                  90                  95      


Met Asn Thr Ile Met Asn Gly Ser Pro Ser Ala Asp Ser Thr Gly Glu 
            100                 105                 110         


Ser Gln Gln Ala Lys Gly Pro Gln Gln Gly Leu Ile Gln Arg Leu Ile 
        115                 120                 125             


Lys Gly Leu Ala Asp Ile Phe Val Pro Ile Ile Pro Ala Ile Val Ala 
    130                 135                 140                 


Gly Gly Leu Leu Met Gly Ile Asn Asn Val Phe Thr Ala Lys Asp Leu 
145                 150                 155                 160 


Phe Glu Glu Gly Lys Thr Leu Leu Asp Leu Tyr Pro Gln Tyr Lys Asp 
                165                 170                 175     


Leu Ala Asp Leu Ile Asn Thr Phe Ala Asn Ala Pro Phe Val Phe Leu 
            180                 185                 190         


Pro Val Leu Leu Gly Phe Ser Ala Thr Arg Lys Phe Gly Gly Asn Pro 
        195                 200                 205             


Phe Leu Gly Ala Thr Leu Gly Met Leu Leu Val His Pro Ala Leu Thr 
    210                 215                 220                 


Asn Ala Tyr Gly Tyr Ala Glu Ala Leu Ala Gly Gly Asn Leu Gln Leu 
225                 230                 235                 240 


Trp Asn Ile Phe Gly Leu Glu Ile Glu Lys Val Gly Tyr Gln Gly Thr 
                245                 250                 255     


Val Ile Pro Val Leu Ile Ala Ala Trp Val Leu Ala Thr Leu Glu Lys 
            260                 265                 270         


Phe Leu Val Lys Val Val Pro Ser Val Leu Asn Asn Leu Val Thr Pro 
        275                 280                 285             


Leu Phe Ser Leu Phe Ile Thr Gly Phe Leu Ala Phe Thr Val Ile Gly 
    290                 295                 300                 


Pro Phe Gly Arg Glu Ala Gly Glu Phe Leu Ser Gln Gly Leu Thr Trp 
305                 310                 315                 320 


Leu Tyr Asp Thr Leu Gly Phe Ile Gly Gly Gly Val Phe Gly Ala Leu 
                325                 330                 335     


Tyr Ala Pro Ile Val Ile Thr Gly Met His Gln Thr Phe Ile Ala Ile 
            340                 345                 350         


Glu Thr Gln Leu Leu Ala Ser Thr Ala Ala Thr Phe Ile Phe Pro Ile 
        355                 360                 365             


Ala Ala Met Ser Asn Ile Ala Gln Gly Ala Ala Cys Leu Ala Val Ala 
    370                 375                 380                 


Val Leu Asn Lys Asp Ala Lys Thr Arg Gly Leu Ala Leu Pro Ser Gly 
385                 390                 395                 400 


Ile Ser Ala Leu Leu Gly Ile Thr Glu Pro Ala Met Phe Gly Val Asn 
                405                 410                 415     


Leu Arg Phe Arg Tyr Pro Phe Tyr Ala Ala Met Leu Gly Ala Gly Ser 
            420                 425                 430         


Ala Ala Ala Phe Ile Ala Phe Phe Asn Val Lys Ala Thr Ala Leu Gly 
        435                 440                 445             


Ala Ala Gly Leu Ile Gly Ile Ala Ser Ile Arg Ala Gly Asp Trp Gly 
    450                 455                 460                 


Met Tyr Ser Val Gly Met Val Ile Ser Phe Cys Val Ala Phe Ala Ala 
465                 470                 475                 480 


Ala Leu Val Leu Gly Ala Arg Ala Asn Ala Lys Glu 
                485                 490         


<210>  5
<211>  1434
<212>  DNA
<213>  E. coli W


<220>
<221>  misc_feature
<222>  (1)..(1434)
<223>  nucleotide sequence of cscA-gene from E. coli W

<400>  5
atgacgcaat ctcgattgca tgcggcgcaa aacgccctag caaaacttca tgagcaccgg     60

ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccgggtggat gaacgatcca    120

aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca tccgatgagc    180

gaacactggg ggccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag    240

catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt    300

agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat    360

ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt    420

attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc    480

gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca    540

ggcaacacgg ggcagatcct gctttatcgc ggcagttcgt tgcgtgaatg gaccttcgat    600

cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc    660

agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac    720

agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt    780

tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa    840

agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg    900

ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca    960

gagagcaatg gcaaacttct acaacgcccg gtacacgaag ctgagtcgtt acgccagcag   1020

catcaatctg tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa   1080

gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta   1140

cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg   1200

cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac   1260

acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg   1320

gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat   1380

gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg ttaa         1434


<210>  6
<211>  477
<212>  PRT
<213>  E. coli W


<220>
<221>  SITE
<222>  (1)..(477)
<223>  amino acid sequence of CscA from E. coli W

<400>  6

Met Thr Gln Ser Arg Leu His Ala Ala Gln Asn Ala Leu Ala Lys Leu 
1               5                   10                  15      


His Glu His Arg Gly Asn Thr Phe Tyr Pro His Phe His Leu Ala Pro 
            20                  25                  30          


Pro Ala Gly Trp Met Asn Asp Pro Asn Gly Leu Ile Trp Phe Asn Asp 
        35                  40                  45              


Arg Tyr His Ala Phe Tyr Gln His His Pro Met Ser Glu His Trp Gly 
    50                  55                  60                  


Pro Met His Trp Gly His Ala Thr Ser Asp Asp Met Ile His Trp Gln 
65                  70                  75                  80  


His Glu Pro Ile Ala Leu Ala Pro Gly Asp Asp Asn Asp Lys Asp Gly 
                85                  90                  95      


Cys Phe Ser Gly Ser Ala Val Asp Asp Asn Gly Val Leu Ser Leu Ile 
            100                 105                 110         


Tyr Thr Gly His Val Trp Leu Asp Gly Ala Gly Asn Asp Asp Ala Ile 
        115                 120                 125             


Arg Glu Val Gln Cys Leu Ala Thr Ser Arg Asp Gly Ile His Phe Glu 
    130                 135                 140                 


Lys Gln Gly Val Ile Leu Thr Pro Pro Glu Gly Ile Met His Phe Arg 
145                 150                 155                 160 


Asp Pro Lys Val Trp Arg Glu Ala Asp Thr Trp Trp Met Val Val Gly 
                165                 170                 175     


Ala Lys Asp Pro Gly Asn Thr Gly Gln Ile Leu Leu Tyr Arg Gly Ser 
            180                 185                 190         


Ser Leu Arg Glu Trp Thr Phe Asp Arg Val Leu Ala His Ala Asp Ala 
        195                 200                 205             


Gly Glu Ser Tyr Met Trp Glu Cys Pro Asp Phe Phe Ser Leu Gly Asp 
    210                 215                 220                 


Gln His Tyr Leu Met Phe Ser Pro Gln Gly Met Asn Ala Glu Gly Tyr 
225                 230                 235                 240 


Ser Tyr Arg Asn Arg Phe Gln Ser Gly Val Ile Pro Gly Met Trp Ser 
                245                 250                 255     


Pro Gly Arg Leu Phe Ala Gln Ser Gly His Phe Thr Glu Leu Asp Asn 
            260                 265                 270         


Gly His Asp Phe Tyr Ala Pro Gln Ser Phe Leu Ala Lys Asp Gly Arg 
        275                 280                 285             


Arg Ile Val Ile Gly Trp Met Asp Met Trp Glu Ser Pro Met Pro Ser 
    290                 295                 300                 


Lys Arg Glu Gly Trp Ala Gly Cys Met Thr Leu Ala Arg Glu Leu Ser 
305                 310                 315                 320 


Glu Ser Asn Gly Lys Leu Leu Gln Arg Pro Val His Glu Ala Glu Ser 
                325                 330                 335     


Leu Arg Gln Gln His Gln Ser Val Ser Pro Arg Thr Ile Ser Asn Lys 
            340                 345                 350         


Tyr Val Leu Gln Glu Asn Ala Gln Ala Val Glu Ile Gln Leu Gln Trp 
        355                 360                 365             


Ala Leu Lys Asn Ser Asp Ala Glu His Tyr Gly Leu Gln Leu Gly Thr 
    370                 375                 380                 


Gly Met Arg Leu Tyr Ile Asp Asn Gln Ser Glu Arg Leu Val Leu Trp 
385                 390                 395                 400 


Arg Tyr Tyr Pro His Glu Asn Leu Asp Gly Tyr Arg Ser Ile Pro Leu 
                405                 410                 415     


Pro Gln Arg Asp Thr Leu Ala Leu Arg Ile Phe Ile Asp Thr Ser Ser 
            420                 425                 430         


Val Glu Val Phe Ile Asn Asp Gly Glu Ala Val Met Ser Ser Arg Ile 
        435                 440                 445             


Tyr Pro Gln Pro Glu Glu Arg Glu Leu Ser Leu Tyr Ala Ser His Gly 
    450                 455                 460                 


Val Ala Val Leu Gln His Gly Ala Leu Trp Leu Leu Gly 
465                 470                 475         


<210>  7
<211>  1203
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1203)
<223>  nucleotide sequence of argD-gene from from strain DD1

<400>  7
atgagccaat atactcgcaa aacattcgat gaagtgatga tccaaaacta cgtacctgcg     60

gattttattc ctgtgaaagg caaaggttgc aaagtttggg atcagcaagg gcgagattat    120

attgatttta ccagcggaat tgccgtaaat gcattgggac attgtcctga cgaaatcgtc    180

gatgtgctga aaaaacaagg cgaaactcta tggcattcaa gcaactggtt caccagtgaa    240

ccgactctgg aacttgcctc caaattggtt gaacatacct ttgccgaacg tgtgatgttt    300

gccaattccg gcggtgaagc caatgaggcg gcgcttaaat tggcgcgtcg ttatgcggtg    360

gataattacg gctatcaaaa agataccatt atttcattca aaaaaagttt ccacggacgt    420

accttattta ccgtcagcgt cggcggtcag gcgaaatatt cggacggatt cggcccgaaa    480

cctgccggaa tcgtgcattt gccgtttaat gatcttgatg cggttaaagc gatgattgat    540

gatcacagct gtgcggtaat cgttgagccg attcagggag aaagcgggat tattccggct    600

accaaagaat ttttacaggg tttgcgtcga ctttgcgacg aaaataacgc tctattgatt    660

tttgacgaag tacaaacggg ggtagggcgt accggttatt tatacgcata tgaaagttat    720

gatgtagtgc cggatattct gacctcgtca aaagcccttg ctaacggttt tccgattagc    780

gcaatgttaa ccaccacaaa aattgccgcc agtttcaaac cgggcgtgca cggcaccaca    840

ttcggcggta atccgttagc ctgtgcggtc ggggcgaaag tgattgagac gattgcaaat    900

ccggcgtttc ttgaaaatgt acaaaaaaca tccgcacttt ttatcagcga attaaacaaa    960

cttaatgaga aatatcactt atttaacgaa gttcgcggtc agggattatt aatcggggcg   1020

gaattaattg aaaaatatca gggcaaagcc tcagagtttg tcaaagcctg tgcggataat   1080

caacttatga ttttagtcgc tgggccgaat gtgctacgtt ttgcgccggc attaaatatt   1140

agtcaacagg aagtggcgga aggatttaaa cgtttagatc aggctctgca aaaatttgct   1200

taa                                                                 1203


<210>  8
<211>  400
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(400)
<223>  amino acid sequence of ArgD from strain DD1

<400>  8

Met Ser Gln Tyr Thr Arg Lys Thr Phe Asp Glu Val Met Ile Gln Asn 
1               5                   10                  15      


Tyr Val Pro Ala Asp Phe Ile Pro Val Lys Gly Lys Gly Cys Lys Val 
            20                  25                  30          


Trp Asp Gln Gln Gly Arg Asp Tyr Ile Asp Phe Thr Ser Gly Ile Ala 
        35                  40                  45              


Val Asn Ala Leu Gly His Cys Pro Asp Glu Ile Val Asp Val Leu Lys 
    50                  55                  60                  


Lys Gln Gly Glu Thr Leu Trp His Ser Ser Asn Trp Phe Thr Ser Glu 
65                  70                  75                  80  


Pro Thr Leu Glu Leu Ala Ser Lys Leu Val Glu His Thr Phe Ala Glu 
                85                  90                  95      


Arg Val Met Phe Ala Asn Ser Gly Gly Glu Ala Asn Glu Ala Ala Leu 
            100                 105                 110         


Lys Leu Ala Arg Arg Tyr Ala Val Asp Asn Tyr Gly Tyr Gln Lys Asp 
        115                 120                 125             


Thr Ile Ile Ser Phe Lys Lys Ser Phe His Gly Arg Thr Leu Phe Thr 
    130                 135                 140                 


Val Ser Val Gly Gly Gln Ala Lys Tyr Ser Asp Gly Phe Gly Pro Lys 
145                 150                 155                 160 


Pro Ala Gly Ile Val His Leu Pro Phe Asn Asp Leu Asp Ala Val Lys 
                165                 170                 175     


Ala Met Ile Asp Asp His Ser Cys Ala Val Ile Val Glu Pro Ile Gln 
            180                 185                 190         


Gly Glu Ser Gly Ile Ile Pro Ala Thr Lys Glu Phe Leu Gln Gly Leu 
        195                 200                 205             


Arg Arg Leu Cys Asp Glu Asn Asn Ala Leu Leu Ile Phe Asp Glu Val 
    210                 215                 220                 


Gln Thr Gly Val Gly Arg Thr Gly Tyr Leu Tyr Ala Tyr Glu Ser Tyr 
225                 230                 235                 240 


Asp Val Val Pro Asp Ile Leu Thr Ser Ser Lys Ala Leu Ala Asn Gly 
                245                 250                 255     


Phe Pro Ile Ser Ala Met Leu Thr Thr Thr Lys Ile Ala Ala Ser Phe 
            260                 265                 270         


Lys Pro Gly Val His Gly Thr Thr Phe Gly Gly Asn Pro Leu Ala Cys 
        275                 280                 285             


Ala Val Gly Ala Lys Val Ile Glu Thr Ile Ala Asn Pro Ala Phe Leu 
    290                 295                 300                 


Glu Asn Val Gln Lys Thr Ser Ala Leu Phe Ile Ser Glu Leu Asn Lys 
305                 310                 315                 320 


Leu Asn Glu Lys Tyr His Leu Phe Asn Glu Val Arg Gly Gln Gly Leu 
                325                 330                 335     


Leu Ile Gly Ala Glu Leu Ile Glu Lys Tyr Gln Gly Lys Ala Ser Glu 
            340                 345                 350         


Phe Val Lys Ala Cys Ala Asp Asn Gln Leu Met Ile Leu Val Ala Gly 
        355                 360                 365             


Pro Asn Val Leu Arg Phe Ala Pro Ala Leu Asn Ile Ser Gln Gln Glu 
    370                 375                 380                 


Val Ala Glu Gly Phe Lys Arg Leu Asp Gln Ala Leu Gln Lys Phe Ala 
385                 390                 395                 400 


<210>  9
<211>  1029
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1029)
<223>  nucleotide sequence of ldhA-gene from strain DD1

<400>  9
ttgacaaaat cagtatgttt aaataaggag ctaactatga aagttgccgt ttacagtact     60

aaaaattatg atcgcaaaca tctggatttg gcgaataaaa aatttaattt tgagcttcat    120

ttctttgatt ttttacttga tgaacaaacc gcgaaaatgg cggagggcgc cgatgccgtc    180

tgtattttcg tcaatgatga tgcgagccgc ccggtgttaa caaagttggc gcaaatcgga    240

gtgaaaatta tcgctttacg ttgtgccggt tttaataatg tggatttgga ggcggcaaaa    300

gagctgggat taaaagtcgt acgggtgcct gcgtattcgc cggaagccgt tgccgagcat    360

gcgatcggat taatgctgac tttaaaccgc cgtatccata aggcttatca gcgtacccgc    420

gatgcgaatt tttctctgga aggattggtc ggttttaata tgttcggcaa aaccgccgga    480

gtgattggta cgggaaaaat cggcttggcg gctattcgca ttttaaaagg cttcggtatg    540

gacgttctgg cgtttgatcc ttttaaaaat ccggcggcgg aagcgttggg cgcaaaatat    600

gtcggtttag acgagcttta tgcaaaatcc catgttatca ctttgcattg cccggctacg    660

gcggataatt atcatttatt aaatgaagcg gcttttaata aaatgcgcga cggtgtaatg    720

attattaata ccagccgcgg cgttttaatt gacagccggg cggcaatcga agcgttaaaa    780

cggcagaaaa tcggcgctct cggtatggat gtttatgaaa atgaacggga tttgtttttc    840

gaggataaat ctaacgatgt tattacggat gatgtattcc gtcgcctttc ttcctgtcat    900

aatgtgcttt ttaccggtca tcaggcgttt ttaacggaag aagcgctgaa taatatcgcc    960

gatgtgactt tatcgaatat tcaggcggtt tccaaaaatg caacgtgcga aaatagcgtt   1020

gaaggctaa                                                           1029


<210>  10
<211>  342
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(342)
<223>  amino acid sequence of LdhA from strain DD1

<400>  10

Met Thr Lys Ser Val Cys Leu Asn Lys Glu Leu Thr Met Lys Val Ala 
1               5                   10                  15      


Val Tyr Ser Thr Lys Asn Tyr Asp Arg Lys His Leu Asp Leu Ala Asn 
            20                  25                  30          


Lys Lys Phe Asn Phe Glu Leu His Phe Phe Asp Phe Leu Leu Asp Glu 
        35                  40                  45              


Gln Thr Ala Lys Met Ala Glu Gly Ala Asp Ala Val Cys Ile Phe Val 
    50                  55                  60                  


Asn Asp Asp Ala Ser Arg Pro Val Leu Thr Lys Leu Ala Gln Ile Gly 
65                  70                  75                  80  


Val Lys Ile Ile Ala Leu Arg Cys Ala Gly Phe Asn Asn Val Asp Leu 
                85                  90                  95      


Glu Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg Val Pro Ala Tyr 
            100                 105                 110         


Ser Pro Glu Ala Val Ala Glu His Ala Ile Gly Leu Met Leu Thr Leu 
        115                 120                 125             


Asn Arg Arg Ile His Lys Ala Tyr Gln Arg Thr Arg Asp Ala Asn Phe 
    130                 135                 140                 


Ser Leu Glu Gly Leu Val Gly Phe Asn Met Phe Gly Lys Thr Ala Gly 
145                 150                 155                 160 


Val Ile Gly Thr Gly Lys Ile Gly Leu Ala Ala Ile Arg Ile Leu Lys 
                165                 170                 175     


Gly Phe Gly Met Asp Val Leu Ala Phe Asp Pro Phe Lys Asn Pro Ala 
            180                 185                 190         


Ala Glu Ala Leu Gly Ala Lys Tyr Val Gly Leu Asp Glu Leu Tyr Ala 
        195                 200                 205             


Lys Ser His Val Ile Thr Leu His Cys Pro Ala Thr Ala Asp Asn Tyr 
    210                 215                 220                 


His Leu Leu Asn Glu Ala Ala Phe Asn Lys Met Arg Asp Gly Val Met 
225                 230                 235                 240 


Ile Ile Asn Thr Ser Arg Gly Val Leu Ile Asp Ser Arg Ala Ala Ile 
                245                 250                 255     


Glu Ala Leu Lys Arg Gln Lys Ile Gly Ala Leu Gly Met Asp Val Tyr 
            260                 265                 270         


Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser Asn Asp Val Ile 
        275                 280                 285             


Thr Asp Asp Val Phe Arg Arg Leu Ser Ser Cys His Asn Val Leu Phe 
    290                 295                 300                 


Thr Gly His Gln Ala Phe Leu Thr Glu Glu Ala Leu Asn Asn Ile Ala 
305                 310                 315                 320 


Asp Val Thr Leu Ser Asn Ile Gln Ala Val Ser Lys Asn Ala Thr Cys 
                325                 330                 335     


Glu Asn Ser Val Glu Gly 
            340         


<210>  11
<211>  741
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(741)
<223>  nucleotide sequence of pflA-gene from strain DD1

<400>  11
atgtcggttt taggacgaat tcattcattt gaaacctgcg ggacagttga cgggccggga     60

atccgcttta ttttattttt acaaggctgc ttaatgcgtt gtaaatactg ccataataga    120

gacacctggg atttgcacgg cggtaaagaa atttccgttg aagaattaat gaaagaagtg    180

gtgacctatc gccattttat gaacgcctcg ggcggcggag ttaccgcttc cggcggtgaa    240

gctattttac aggcggaatt tgtacgggac tggttcagag cctgccataa agaaggaatt    300

aatacttgct tggataccaa cggtttcgtc cgtcatcatg atcatattat tgatgaattg    360

attgatgaca cggatcttgt gttgcttgac ctgaaagaaa tgaatgaacg ggttcacgaa    420

agcctgattg gcgtgccgaa taaaagagtg ctcgaattcg caaaatattt agcggatcga    480

aatcagcgta cctggatccg ccatgttgta gtgccgggtt atacagatag tgacgaagat    540

ttgcacatgc tggggaattt cattaaagat atgaagaata tcgaaaaagt ggaattatta    600

ccttatcacc gtctaggcgc ccataaatgg gaagtactcg gcgataaata cgagcttgaa    660

gatgtaaaac cgccgacaaa agaattaatg gagcatgtta aggggttgct tgcaggctac    720

gggcttaatg tgacatatta g                                              741


<210>  12
<211>  246
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(246)
<223>  amino acid sequence of PflA from strain DD1

<400>  12

Met Ser Val Leu Gly Arg Ile His Ser Phe Glu Thr Cys Gly Thr Val 
1               5                   10                  15      


Asp Gly Pro Gly Ile Arg Phe Ile Leu Phe Leu Gln Gly Cys Leu Met 
            20                  25                  30          


Arg Cys Lys Tyr Cys His Asn Arg Asp Thr Trp Asp Leu His Gly Gly 
        35                  40                  45              


Lys Glu Ile Ser Val Glu Glu Leu Met Lys Glu Val Val Thr Tyr Arg 
    50                  55                  60                  


His Phe Met Asn Ala Ser Gly Gly Gly Val Thr Ala Ser Gly Gly Glu 
65                  70                  75                  80  


Ala Ile Leu Gln Ala Glu Phe Val Arg Asp Trp Phe Arg Ala Cys His 
                85                  90                  95      


Lys Glu Gly Ile Asn Thr Cys Leu Asp Thr Asn Gly Phe Val Arg His 
            100                 105                 110         


His Asp His Ile Ile Asp Glu Leu Ile Asp Asp Thr Asp Leu Val Leu 
        115                 120                 125             


Leu Asp Leu Lys Glu Met Asn Glu Arg Val His Glu Ser Leu Ile Gly 
    130                 135                 140                 


Val Pro Asn Lys Arg Val Leu Glu Phe Ala Lys Tyr Leu Ala Asp Arg 
145                 150                 155                 160 


Asn Gln Arg Thr Trp Ile Arg His Val Val Val Pro Gly Tyr Thr Asp 
                165                 170                 175     


Ser Asp Glu Asp Leu His Met Leu Gly Asn Phe Ile Lys Asp Met Lys 
            180                 185                 190         


Asn Ile Glu Lys Val Glu Leu Leu Pro Tyr His Arg Leu Gly Ala His 
        195                 200                 205             


Lys Trp Glu Val Leu Gly Asp Lys Tyr Glu Leu Glu Asp Val Lys Pro 
    210                 215                 220                 


Pro Thr Lys Glu Leu Met Glu His Val Lys Gly Leu Leu Ala Gly Tyr 
225                 230                 235                 240 


Gly Leu Asn Val Thr Tyr 
                245     


<210>  13
<211>  2313
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(2313)
<223>  nucleotide sequence of pflD-gene from strain DD1

<400>  13
atggctgaat taacagaagc tcaaaaaaaa gcatgggaag gattcgttcc cggtgaatgg     60

caaaacggcg taaatttacg tgactttatc caaaaaaact atactccgta tgaaggtgac    120

gaatcattct tagctgatgc gactcctgca accagcgagt tgtggaacag cgtgatggaa    180

ggcatcaaaa tcgaaaacaa aactcacgca cctttagatt tcgacgaaca tactccgtca    240

actatcactt ctcacaagcc tggttatatc aataaagatt tagaaaaaat cgttggtctt    300

caaacagacg ctccgttaaa acgtgcaatt atgccgtacg gcggtatcaa aatgatcaaa    360

ggttcttgcg aagtttacgg tcgtaaatta gatccgcaag tagaatttat tttcaccgaa    420

tatcgtaaaa cccataacca aggcgtattc gacgtttata cgccggatat tttacgctgc    480

cgtaaatcag gcgtgttaac cggtttaccg gatgcttacg gtcgtggtcg tattatcggt    540

gactaccgtc gtttagcggt atacggtatt gattacctga tgaaagataa aaaagcccaa    600

ttcgattcat tacaaccgcg tttggaagcg ggcgaagaca ttcaggcaac tatccaatta    660

cgtgaagaaa ttgccgaaca acaccgcgct ttaggcaaaa tcaaagaaat ggcggcatct    720

tacggttacg acatttccgg ccctgcgaca aacgcacagg aagcaatcca atggacatat    780

tttgcttatc tggcagcggt taaatcacaa aacggtgcgg caatgtcatt cggtcgtacg    840

tctacattct tagatatcta tatcgaacgt gacttaaaac gcggtttaat cactgaacaa    900

caggcgcagg aattaatgga ccacttagta atgaaattac gtatggttcg tttcttacgt    960

acgccggaat acgatcaatt attctcaggc gacccgatgt gggcaaccga aactatcgcc   1020

ggtatgggct tagacggtcg tccgttggta actaaaaaca gcttccgcgt attacatact   1080

ttatacacta tgggtacttc tccggaacca aacttaacta ttctttggtc cgaacaatta   1140

cctgaagcgt tcaaacgttt ctgtgcgaaa gtatctattg atacttcctc cgtacaatac   1200

gaaaatgatg acttaatgcg tcctgacttc aacaacgatg actatgcaat cgcatgctgc   1260

gtatcaccga tggtcgtagg taaacaaatg caattcttcg gtgcgcgcgc aaacttagct   1320

aaaactatgt tatacgcaat taacggcggt atcgatgaga aaaatggtat gcaagtcggt   1380

cctaaaactg cgccgattac agacgaagta ttgaatttcg ataccgtaat cgaacgtatg   1440

gacagtttca tggactggtt ggcgactcaa tatgtaaccg cattgaacat catccacttc   1500

atgcacgata aatatgcata tgaagcggca ttgatggcgt tccacgatcg cgacgtattc   1560

cgtacaatgg cttgcggtat cgcgggtctt tccgtggctg cggactcatt atccgcaatc   1620

aaatatgcga aagttaaacc gattcgcggc gacatcaaag ataaagacgg taatgtcgtg   1680

gcctcgaatg ttgctatcga cttcgaaatt gaaggcgaat atccgcaatt cggtaacaat   1740

gatccgcgtg ttgatgattt agcggtagac ttagttgaac gtttcatgaa aaaagttcaa   1800

aaacacaaaa cttaccgcaa cgcaactccg acacaatcta tcctgactat cacttctaac   1860

gtggtatacg gtaagaaaac cggtaatact ccggacggtc gtcgagcagg cgcgccattc   1920

ggaccgggtg caaacccaat gcacggtcgt gaccaaaaag gtgcggttgc ttcacttact   1980

tctgtggcta aacttccgtt cgcttacgcg aaagacggta tttcatatac cttctctatc   2040

gtaccgaacg cattaggtaa agatgacgaa gcgcaaaaac gcaaccttgc cggtttaatg   2100

gacggttatt tccatcatga agcgacagtg gaaggcggtc aacacttgaa tgttaacgtt   2160

cttaaccgtg aaatgttgtt agacgcgatg gaaaatccgg aaaaataccc gcaattaacc   2220

attcgtgttt caggttacgc ggttcgtttc aactcattaa ctaaagagca acaacaagac   2280

gtcatcactc gtacgtttac acaatcaatg taa                                2313


<210>  14
<211>  770
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(770)
<223>  amino acid of PflD from strain DD1

<400>  14

Met Ala Glu Leu Thr Glu Ala Gln Lys Lys Ala Trp Glu Gly Phe Val 
1               5                   10                  15      


Pro Gly Glu Trp Gln Asn Gly Val Asn Leu Arg Asp Phe Ile Gln Lys 
            20                  25                  30          


Asn Tyr Thr Pro Tyr Glu Gly Asp Glu Ser Phe Leu Ala Asp Ala Thr 
        35                  40                  45              


Pro Ala Thr Ser Glu Leu Trp Asn Ser Val Met Glu Gly Ile Lys Ile 
    50                  55                  60                  


Glu Asn Lys Thr His Ala Pro Leu Asp Phe Asp Glu His Thr Pro Ser 
65                  70                  75                  80  


Thr Ile Thr Ser His Lys Pro Gly Tyr Ile Asn Lys Asp Leu Glu Lys 
                85                  90                  95      


Ile Val Gly Leu Gln Thr Asp Ala Pro Leu Lys Arg Ala Ile Met Pro 
            100                 105                 110         


Tyr Gly Gly Ile Lys Met Ile Lys Gly Ser Cys Glu Val Tyr Gly Arg 
        115                 120                 125             


Lys Leu Asp Pro Gln Val Glu Phe Ile Phe Thr Glu Tyr Arg Lys Thr 
    130                 135                 140                 


His Asn Gln Gly Val Phe Asp Val Tyr Thr Pro Asp Ile Leu Arg Cys 
145                 150                 155                 160 


Arg Lys Ser Gly Val Leu Thr Gly Leu Pro Asp Ala Tyr Gly Arg Gly 
                165                 170                 175     


Arg Ile Ile Gly Asp Tyr Arg Arg Leu Ala Val Tyr Gly Ile Asp Tyr 
            180                 185                 190         


Leu Met Lys Asp Lys Lys Ala Gln Phe Asp Ser Leu Gln Pro Arg Leu 
        195                 200                 205             


Glu Ala Gly Glu Asp Ile Gln Ala Thr Ile Gln Leu Arg Glu Glu Ile 
    210                 215                 220                 


Ala Glu Gln His Arg Ala Leu Gly Lys Ile Lys Glu Met Ala Ala Ser 
225                 230                 235                 240 


Tyr Gly Tyr Asp Ile Ser Gly Pro Ala Thr Asn Ala Gln Glu Ala Ile 
                245                 250                 255     


Gln Trp Thr Tyr Phe Ala Tyr Leu Ala Ala Val Lys Ser Gln Asn Gly 
            260                 265                 270         


Ala Ala Met Ser Phe Gly Arg Thr Ser Thr Phe Leu Asp Ile Tyr Ile 
        275                 280                 285             


Glu Arg Asp Leu Lys Arg Gly Leu Ile Thr Glu Gln Gln Ala Gln Glu 
    290                 295                 300                 


Leu Met Asp His Leu Val Met Lys Leu Arg Met Val Arg Phe Leu Arg 
305                 310                 315                 320 


Thr Pro Glu Tyr Asp Gln Leu Phe Ser Gly Asp Pro Met Trp Ala Thr 
                325                 330                 335     


Glu Thr Ile Ala Gly Met Gly Leu Asp Gly Arg Pro Leu Val Thr Lys 
            340                 345                 350         


Asn Ser Phe Arg Val Leu His Thr Leu Tyr Thr Met Gly Thr Ser Pro 
        355                 360                 365             


Glu Pro Asn Leu Thr Ile Leu Trp Ser Glu Gln Leu Pro Glu Ala Phe 
    370                 375                 380                 


Lys Arg Phe Cys Ala Lys Val Ser Ile Asp Thr Ser Ser Val Gln Tyr 
385                 390                 395                 400 


Glu Asn Asp Asp Leu Met Arg Pro Asp Phe Asn Asn Asp Asp Tyr Ala 
                405                 410                 415     


Ile Ala Cys Cys Val Ser Pro Met Val Val Gly Lys Gln Met Gln Phe 
            420                 425                 430         


Phe Gly Ala Arg Ala Asn Leu Ala Lys Thr Met Leu Tyr Ala Ile Asn 
        435                 440                 445             


Gly Gly Ile Asp Glu Lys Asn Gly Met Gln Val Gly Pro Lys Thr Ala 
    450                 455                 460                 


Pro Ile Thr Asp Glu Val Leu Asn Phe Asp Thr Val Ile Glu Arg Met 
465                 470                 475                 480 


Asp Ser Phe Met Asp Trp Leu Ala Thr Gln Tyr Val Thr Ala Leu Asn 
                485                 490                 495     


Ile Ile His Phe Met His Asp Lys Tyr Ala Tyr Glu Ala Ala Leu Met 
            500                 505                 510         


Ala Phe His Asp Arg Asp Val Phe Arg Thr Met Ala Cys Gly Ile Ala 
        515                 520                 525             


Gly Leu Ser Val Ala Ala Asp Ser Leu Ser Ala Ile Lys Tyr Ala Lys 
    530                 535                 540                 


Val Lys Pro Ile Arg Gly Asp Ile Lys Asp Lys Asp Gly Asn Val Val 
545                 550                 555                 560 


Ala Ser Asn Val Ala Ile Asp Phe Glu Ile Glu Gly Glu Tyr Pro Gln 
                565                 570                 575     


Phe Gly Asn Asn Asp Pro Arg Val Asp Asp Leu Ala Val Asp Leu Val 
            580                 585                 590         


Glu Arg Phe Met Lys Lys Val Gln Lys His Lys Thr Tyr Arg Asn Ala 
        595                 600                 605             


Thr Pro Thr Gln Ser Ile Leu Thr Ile Thr Ser Asn Val Val Tyr Gly 
    610                 615                 620                 


Lys Lys Thr Gly Asn Thr Pro Asp Gly Arg Arg Ala Gly Ala Pro Phe 
625                 630                 635                 640 


Gly Pro Gly Ala Asn Pro Met His Gly Arg Asp Gln Lys Gly Ala Val 
                645                 650                 655     


Ala Ser Leu Thr Ser Val Ala Lys Leu Pro Phe Ala Tyr Ala Lys Asp 
            660                 665                 670         


Gly Ile Ser Tyr Thr Phe Ser Ile Val Pro Asn Ala Leu Gly Lys Asp 
        675                 680                 685             


Asp Glu Ala Gln Lys Arg Asn Leu Ala Gly Leu Met Asp Gly Tyr Phe 
    690                 695                 700                 


His His Glu Ala Thr Val Glu Gly Gly Gln His Leu Asn Val Asn Val 
705                 710                 715                 720 


Leu Asn Arg Glu Met Leu Leu Asp Ala Met Glu Asn Pro Glu Lys Tyr 
                725                 730                 735     


Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr Ala Val Arg Phe Asn Ser 
            740                 745                 750         


Leu Thr Lys Glu Gln Gln Gln Asp Val Ile Thr Arg Thr Phe Thr Gln 
        755                 760                 765             


Ser Met 
    770 


<210>  15
<211>  600
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(600)
<223>  nucleotide sequence of wcaJ-gene from strain DD1

<400>  15
atgataaaac gccttttcga tattgttgtc gcattgatag cattgatttt gttttcgccc     60

ttatatttgt ttgtggctta taaggtaaaa caaaatttgg gatcaccggt gttatttaaa    120

caaacccgcc ccggattgca tggtaaaccc tttgagatga ttaagttcag aacaatgaaa    180

gacggcgcag atgaaaacgg taatattttg ccggatgcgg agcgcttaac acctttcggc    240

aaaatgttgc gcgctaccag tctggacgag ttgccggaac tttggaatgt attaaaaggt    300

gatatgagtc tggtggggcc gcgtcctcta ctgatggaat atttgccgct gtataacgaa    360

agacaggcta agcgccatga agtgaaaccc ggaattaccg gttatgcaca ggtaaacggt    420

cgcaatgcca tcagttggga gcagaaattt gaattggatg cctggtatgt tgaacatcaa    480

tccttgtggc tggatttgaa aattatcgca aagaccatcc aaaaagtgat cgcaaaagac    540

gatattaatg cggcagatga tgccaccatg cctaaatttg aagggaataa aaaatcatga    600


<210>  16
<211>  199
<212>  PRT
<213>  Basfia succiniciproducenss


<220>
<221>  SITE
<222>  (1)..(199)
<223>  amino acid sequence of the enzyme encoded by the above wcaJ-gene

<400>  16

Met Ile Lys Arg Leu Phe Asp Ile Val Val Ala Leu Ile Ala Leu Ile 
1               5                   10                  15      


Leu Phe Ser Pro Leu Tyr Leu Phe Val Ala Tyr Lys Val Lys Gln Asn 
            20                  25                  30          


Leu Gly Ser Pro Val Leu Phe Lys Gln Thr Arg Pro Gly Leu His Gly 
        35                  40                  45              


Lys Pro Phe Glu Met Ile Lys Phe Arg Thr Met Lys Asp Gly Ala Asp 
    50                  55                  60                  


Glu Asn Gly Asn Ile Leu Pro Asp Ala Glu Arg Leu Thr Pro Phe Gly 
65                  70                  75                  80  


Lys Met Leu Arg Ala Thr Ser Leu Asp Glu Leu Pro Glu Leu Trp Asn 
                85                  90                  95      


Val Leu Lys Gly Asp Met Ser Leu Val Gly Pro Arg Pro Leu Leu Met 
            100                 105                 110         


Glu Tyr Leu Pro Leu Tyr Asn Glu Arg Gln Ala Lys Arg His Glu Val 
        115                 120                 125             


Lys Pro Gly Ile Thr Gly Tyr Ala Gln Val Asn Gly Arg Asn Ala Ile 
    130                 135                 140                 


Ser Trp Glu Gln Lys Phe Glu Leu Asp Ala Trp Tyr Val Glu His Gln 
145                 150                 155                 160 


Ser Leu Trp Leu Asp Leu Lys Ile Ile Ala Lys Thr Ile Gln Lys Val 
                165                 170                 175     


Ile Ala Lys Asp Asp Ile Asn Ala Ala Asp Asp Ala Thr Met Pro Lys 
            180                 185                 190         


Phe Glu Gly Asn Lys Lys Ser 
        195                 


<210>  17
<211>  601
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(601)
<223>  nucleotide sequence of wcaJ-gene from strain DD1 with insertion 
       of cytosine between nucleotides 81 and 82

<400>  17
atgataaaac gccttttcga tattgttgtc gcattgatag cattgatttt gttttcgccc     60

ttatatttgt ttgtggctta tcaaggtaaa acaaaatttg ggatcaccgg tgttatttaa    120

acaaacccgc cccggattgc atggtaaacc ctttgagatg attaagttca gaacaatgaa    180

agacggcgca gatgaaaacg gtaatatttt gccggatgcg gagcgcttaa cacctttcgg    240

caaaatgttg cgcgctacca gtctggacga gttgccggaa ctttggaatg tattaaaagg    300

tgatatgagt ctggtggggc cgcgtcctct actgatggaa tatttgccgc tgtataacga    360

aagacaggct aagcgccatg aagtgaaacc cggaattacc ggttatgcac aggtaaacgg    420

tcgcaatgcc atcagttggg agcagaaatt tgaattggat gcctggtatg ttgaacatca    480

atccttgtgg ctggatttga aaattatcgc aaagaccatc caaaaagtga tcgcaaaaga    540

cgatattaat gcggcagatg atgccaccat gcctaaattt gaagggaata aaaaatcatg    600

a                                                                    601


<210>  18
<211>  1440
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1440)
<223>  nucleotide sequence of pykA-gene from strain DD1

<400>  18
atgtccagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggcgg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctcg cattagttcc   1200

ggtttaccaa tctttgcatt gtcacgtaac gaatctacat taaacttatg cgcattatat   1260

cgtggtgtga caccggttca ttttgataaa gacagccgta cctcagaagg tgcgacagcg   1320

gcggttcaat tattaaaaga cgaaggtttc ttagtgtctg gcgatttagt gttattaact   1380

cagggcgacg caagcagttc tagcggtact aacctttgcc gtacattgat tgttgaataa   1440


<210>  19
<211>  479
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(479)
<223>  amino acid sequence of PykA from strain DD1

<400>  19

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Cys Thr Met Gly Pro 
1               5                   10                  15      


Ala Thr Asp Lys Gly Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala 
            20                  25                  30          


Asn Val Val Arg Met Asn Phe Ser His Gly Thr Pro Glu Asp His Ile 
        35                  40                  45              


Gly Arg Ala Glu Lys Val Arg Glu Ile Ala His Lys Leu Gly Lys His 
    50                  55                  60                  


Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr 
65                  70                  75                  80  


Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Ile Leu 
                85                  90                  95      


Asp Ala Glu Met Pro Lys Gly Glu Gly Asn Gln Glu Ala Val Gly Leu 
            100                 105                 110         


Asp Tyr Lys Thr Leu Pro Gln Asp Val Val Pro Gly Asp Ile Leu Leu 
        115                 120                 125             


Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Ala Thr Glu Gly Ala 
    130                 135                 140                 


Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys 
145                 150                 155                 160 


Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Ala Asp Ala Leu Thr Glu 
                165                 170                 175     


Lys Asp Lys Ala Asp Ile Ile Thr Ala Ala Arg Ile Gly Val Asp Tyr 
            180                 185                 190         


Leu Ala Val Ser Phe Pro Arg Ser Ser Ala Asp Leu Asn Tyr Ala Arg 
        195                 200                 205             


Gln Leu Ala Lys Asp Ala Gly Leu Asp Ala Lys Ile Val Ala Lys Val 
    210                 215                 220                 


Glu Arg Ala Glu Thr Val Glu Thr Asp Glu Ala Met Asp Asp Ile Ile 
225                 230                 235                 240 


Asn Ala Ala Asp Val Ile Met Val Ala Arg Gly Asp Leu Gly Val Glu 
                245                 250                 255     


Ile Gly Asp Pro Glu Leu Val Gly Val Gln Lys Lys Leu Ile Arg Arg 
            260                 265                 270         


Ser Arg Gln Leu Asn Arg Val Val Ile Thr Ala Thr Gln Met Met Glu 
        275                 280                 285             


Ser Met Ile Ser Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val 
    290                 295                 300                 


Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu 
305                 310                 315                 320 


Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Lys 
                325                 330                 335     


Val Ala Leu Gly Ala Glu Lys Met Pro Ser Ile Asn Val Ser Lys His 
            340                 345                 350         


Arg Met Asn Val Gln Phe Glu Ser Ile Glu Glu Ser Val Ala Met Ser 
        355                 360                 365             


Ala Met Tyr Ala Ala Asn His Met Arg Gly Val Ala Ala Ile Ile Thr 
    370                 375                 380                 


Leu Thr Ser Ser Gly Arg Thr Ala Arg Leu Met Ser Arg Ile Ser Ser 
385                 390                 395                 400 


Gly Leu Pro Ile Phe Ala Leu Ser Arg Asn Glu Ser Thr Leu Asn Leu 
                405                 410                 415     


Cys Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Lys Asp Ser 
            420                 425                 430         


Arg Thr Ser Glu Gly Ala Thr Ala Ala Val Gln Leu Leu Lys Asp Glu 
        435                 440                 445             


Gly Phe Leu Val Ser Gly Asp Leu Val Leu Leu Thr Gln Gly Asp Ala 
    450                 455                 460                 


Ser Ser Ser Ser Gly Thr Asn Leu Cys Arg Thr Leu Ile Val Glu 
465                 470                 475                 


<210>  20
<211>  1695
<212>  DNA
<213>  Basfia succiniciproducens


<220>
<221>  misc_feature
<222>  (1)..(1695)
<223>  nucleotide sequence of fruA-gene from strain DD1

<400>  20
ttgaaggata agccgatgaa tatttttctt acgcaatcac caaatttagg tcgtgcaaaa     60

gcgtttttat tgcaccaggt tttggctgcc gcagtaaaac aacaaaatca tcaactggta    120

gaaaatgccg aacaagcgga tttagcgatt gttttcggta aaactttgcc gaatttgacc    180

gcacttttag gtaaaaaagt gtatttggcg gatgaagaac aagcgttgaa tgcgcctgaa    240

aataccgtcg cgcaggcatt aaccgaggct gtggattatg ttcaaccggc gcaacaggac    300

gtgcaacccg caactgcttc cggtatgaaa aatatcgtgg cggttaccgc ttgtccgacc    360

ggggtggcgc acacctttat gtctgccgag gcgattacaa cctactgcca acagcaaggt    420

tggaatgtaa aagtggaaac cagaggtcaa gtcggtgcga acaatattat ttctgcggaa    480

gatgtggcgg cggccgattt agtctttatc gctacggata ttaatgtgga tttaagcaaa    540

ttcaaaggaa aaccgatgta tcgtacttca acgggcttag cattgaagaa aaccgcacag    600

gaatttgata aagcctttaa agaagcgacg atttatcagg gtgaagaaac tacaaccacc    660

acagaaacac aaacttcagg cgagaaaaaa ggtgtatata aacatcttat gaccggggtt    720

tcccatatgt taccgcttgt cgttgccggc ggtttattga ttgctatttc gtttatgttc    780

ggtattgagg cgtttaaaga cgaaaacatc gcaggcggct tgccgaaagc attaatggat    840

atcggcggcg gtgcggcgtt ccacttaatg attgccgtat ttgcaggtta tgttgcattc    900

tctattgcag accgtccggg gttagccgta ggtcttatcg gcggtatgct tgccacatcc    960

gccggtgccg gtattttggg cggtattatc gcgggttttc ttgccggtta tgtagtgaaa   1020

ttcctgaatg atgccattca actgccagcc agtttaactt cgttaaaacc gattttaatt   1080

ctgcctttat taggttcggc gatcgtcggc ttggccatga tttatttatt aaatccaccg   1140

gttgctgcgg caatgaatgc gctaaccgaa tggttaaaag gtttgggctc ggcaaacgcg   1200

ctggtgttgg gtgcgattct tggcggtatg atgtgtatcg atatgggcgg tccggtaaac   1260

aaagccgctt atgtattcgg tacgggcatg attggttcac aggtttatac gccgatggct   1320

gcggtaatgg ctgcgggtat ggtaccgcct ttaggaatgg cgattgccac ctggattgcg   1380

cgcgctaaat ttaacgcaag ccaacgtgat gcgggtaaag cttcattcgt actaggttta   1440

tgctttattt ccgaaggtgc gttaccgttt gttgccgccg accctgtacg cgtgattgtt   1500

tcaagtgtaa ttggcggagc cattgccggc gcaatttcta tgagccttgc cattacgctg   1560

caagcgcctc acggcggttt attcgtgatt ccgtttgtgt cgcaaccgtt aatgtatttg   1620

ggtgcgattg ccgtaggcgc cttaacaacc ggcgttcttt acgcaattat caaaccgaaa   1680

caagctgcgg aataa                                                    1695


<210>  21
<211>  564
<212>  PRT
<213>  Basfia succiniciproducens


<220>
<221>  SITE
<222>  (1)..(564)
<223>  amino acid sequence of the enzyme encoded by the above fruA-gene

<400>  21

Met Lys Asp Lys Pro Met Asn Ile Phe Leu Thr Gln Ser Pro Asn Leu 
1               5                   10                  15      


Gly Arg Ala Lys Ala Phe Leu Leu His Gln Val Leu Ala Ala Ala Val 
            20                  25                  30          


Lys Gln Gln Asn His Gln Leu Val Glu Asn Ala Glu Gln Ala Asp Leu 
        35                  40                  45              


Ala Ile Val Phe Gly Lys Thr Leu Pro Asn Leu Thr Ala Leu Leu Gly 
    50                  55                  60                  


Lys Lys Val Tyr Leu Ala Asp Glu Glu Gln Ala Leu Asn Ala Pro Glu 
65                  70                  75                  80  


Asn Thr Val Ala Gln Ala Leu Thr Glu Ala Val Asp Tyr Val Gln Pro 
                85                  90                  95      


Ala Gln Gln Asp Val Gln Pro Ala Thr Ala Ser Gly Met Lys Asn Ile 
            100                 105                 110         


Val Ala Val Thr Ala Cys Pro Thr Gly Val Ala His Thr Phe Met Ser 
        115                 120                 125             


Ala Glu Ala Ile Thr Thr Tyr Cys Gln Gln Gln Gly Trp Asn Val Lys 
    130                 135                 140                 


Val Glu Thr Arg Gly Gln Val Gly Ala Asn Asn Ile Ile Ser Ala Glu 
145                 150                 155                 160 


Asp Val Ala Ala Ala Asp Leu Val Phe Ile Ala Thr Asp Ile Asn Val 
                165                 170                 175     


Asp Leu Ser Lys Phe Lys Gly Lys Pro Met Tyr Arg Thr Ser Thr Gly 
            180                 185                 190         


Leu Ala Leu Lys Lys Thr Ala Gln Glu Phe Asp Lys Ala Phe Lys Glu 
        195                 200                 205             


Ala Thr Ile Tyr Gln Gly Glu Glu Thr Thr Thr Thr Thr Glu Thr Gln 
    210                 215                 220                 


Thr Ser Gly Glu Lys Lys Gly Val Tyr Lys His Leu Met Thr Gly Val 
225                 230                 235                 240 


Ser His Met Leu Pro Leu Val Val Ala Gly Gly Leu Leu Ile Ala Ile 
                245                 250                 255     


Ser Phe Met Phe Gly Ile Glu Ala Phe Lys Asp Glu Asn Ile Ala Gly 
            260                 265                 270         


Gly Leu Pro Lys Ala Leu Met Asp Ile Gly Gly Gly Ala Ala Phe His 
        275                 280                 285             


Leu Met Ile Ala Val Phe Ala Gly Tyr Val Ala Phe Ser Ile Ala Asp 
    290                 295                 300                 


Arg Pro Gly Leu Ala Val Gly Leu Ile Gly Gly Met Leu Ala Thr Ser 
305                 310                 315                 320 


Ala Gly Ala Gly Ile Leu Gly Gly Ile Ile Ala Gly Phe Leu Ala Gly 
                325                 330                 335     


Tyr Val Val Lys Phe Leu Asn Asp Ala Ile Gln Leu Pro Ala Ser Leu 
            340                 345                 350         


Thr Ser Leu Lys Pro Ile Leu Ile Leu Pro Leu Leu Gly Ser Ala Ile 
        355                 360                 365             


Val Gly Leu Ala Met Ile Tyr Leu Leu Asn Pro Pro Val Ala Ala Ala 
    370                 375                 380                 


Met Asn Ala Leu Thr Glu Trp Leu Lys Gly Leu Gly Ser Ala Asn Ala 
385                 390                 395                 400 


Leu Val Leu Gly Ala Ile Leu Gly Gly Met Met Cys Ile Asp Met Gly 
                405                 410                 415     


Gly Pro Val Asn Lys Ala Ala Tyr Val Phe Gly Thr Gly Met Ile Gly 
            420                 425                 430         


Ser Gln Val Tyr Thr Pro Met Ala Ala Val Met Ala Ala Gly Met Val 
        435                 440                 445             


Pro Pro Leu Gly Met Ala Ile Ala Thr Trp Ile Ala Arg Ala Lys Phe 
    450                 455                 460                 


Asn Ala Ser Gln Arg Asp Ala Gly Lys Ala Ser Phe Val Leu Gly Leu 
465                 470                 475                 480 


Cys Phe Ile Ser Glu Gly Ala Leu Pro Phe Val Ala Ala Asp Pro Val 
                485                 490                 495     


Arg Val Ile Val Ser Ser Val Ile Gly Gly Ala Ile Ala Gly Ala Ile 
            500                 505                 510         


Ser Met Ser Leu Ala Ile Thr Leu Gln Ala Pro His Gly Gly Leu Phe 
        515                 520                 525             


Val Ile Pro Phe Val Ser Gln Pro Leu Met Tyr Leu Gly Ala Ile Ala 
    530                 535                 540                 


Val Gly Ala Leu Thr Thr Gly Val Leu Tyr Ala Ile Ile Lys Pro Lys 
545                 550                 555                 560 


Gln Ala Ala Glu 
                


<210>  22
<211>  4285
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB

<400>  22
tcgagaggcc tgacgtcggg cccggtacca cgcgtcatat gactagttcg gacctaggga     60

tatcgtcgac atcgatgctc ttctgcgtta attaacaatt gggatcctct agactccata    120

ggccgctttc ctggctttgc ttccagatgt atgctctcct ccggagagta ccgtgacttt    180

attttcggca caaatacagg ggtcgatgga taaatacggc gatagtttcc tgacggatga    240

tccgtatgta ccggcggaag acaagctgca aacctgtcag atggagattg atttaatggc    300

ggatgtgctg agagcaccgc cccgtgaatc cgcagaactg atccgctatg tgtttgcgga    360

tgattggccg gaataaataa agccgggctt aatacagatt aagcccgtat agggtattat    420

tactgaatac caaacagctt acggaggacg gaatgttacc cattgagaca accagactgc    480

cttctgatta ttaatatttt tcactattaa tcagaaggaa taaccatgaa ttttacccgg    540

attgacctga atacctggaa tcgcagggaa cactttgccc tttatcgtca gcagattaaa    600

tgcggattca gcctgaccac caaactcgat attaccgctt tgcgtaccgc actggcggag    660

acaggttata agttttatcc gctgatgatt tacctgatct cccgggctgt taatcagttt    720

ccggagttcc ggatggcact gaaagacaat gaacttattt actgggacca gtcagacccg    780

gtctttactg tctttcataa agaaaccgaa acattctctg cactgtcctg ccgttatttt    840

ccggatctca gtgagtttat ggcaggttat aatgcggtaa cggcagaata tcagcatgat    900

accagattgt ttccgcaggg aaatttaccg gagaatcacc tgaatatatc atcattaccg    960

tgggtgagtt ttgacgggat ttaacctgaa catcaccgga aatgatgatt attttgcccc   1020

ggtttttacg atggcaaagt ttcagcagga aggtgaccgc gtattattac ctgtttctgt   1080

acaggttcat catgcagtct gtgatggctt tcatgcagca cggtttatta atacacttca   1140

gctgatgtgt gataacatac tgaaataaat taattaattc tgtatttaag ccaccgtatc   1200

cggcaggaat ggtggctttt tttttatatt ttaaccgtaa tctgtaattt cgtttcagac   1260

tggttcagga tgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc   1320

catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca   1380

agcactagcg gcgcgccggc cggcccggtg tgaaataccg cacagatgcg taaggagaaa   1440

ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg   1500

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg   1560

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa   1620

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg   1680

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc   1740

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc   1800

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc   1860

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg   1920

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc   1980

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga   2040

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc   2100

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac   2160

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg   2220

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc   2280

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa   2340

ggccggccgc ggccgccatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg   2400

tccttgttca aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag   2460

gaagctcggc gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct   2520

tgtaatcacg acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt   2580

tacatcgtta ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta   2640

tgggccagtt aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc   2700

gtcaatcgtc atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt   2760

aaagacgttc gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat   2820

cacttttttc agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa   2880

ctcagccgtg cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga   2940

tgtgcttttg ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc   3000

agttccagtg tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg   3060

atctctcagc gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac   3120

attttgatac gtttttccgt caccgtcaaa gattgattta taatcctcta caccgttgat   3180

gttcaaagag ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg tttgtttgcc   3240

gtaatgttta ccggagaaat cagtgtagaa taaacggatt tttccgtcag atgtaaatgt   3300

ggctgaacct gaccattctt gtgtttggtc ttttaggata gaatcatttg catcgaattt   3360

gtcgctgtct ttaaagacgc ggccagcgtt tttccagctg tcaatagaag tttcgccgac   3420

tttttgatag aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc cggctaatgc   3480

aaagacgatg tggtagccgt gatagtttgc gacagtgccg tcagcgtttt gtaatggcca   3540

gctgtcccaa acgtccaggc cttttgcaga agagatattt ttaattgtgg acgaatcaaa   3600

ttcagaaact tgatattttt catttttttg ctgttcaggg atttgcagca tatcatggcg   3660

tgtaatatgg gaaatgccgt atgtttcctt atatggcttt tggttcgttt ctttcgcaaa   3720

cgcttgagtt gcgcctcctg ccagcagtgc ggtagtaaag gttaatactg ttgcttgttt   3780

tgcaaacttt ttgatgttca tcgttcatgt ctcctttttt atgtactgtg ttagcggtct   3840

gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata aaaaaagacc   3900

taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat tttaggtctt   3960

gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc tattagactc   4020

tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc ataaaaggat   4080

ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat tctctgtatt   4140

ttttatagtt tctgttgcat gggcataaag ttgccttttt aatcacaatt cagaaaatat   4200

cataatatct catttcacta aataatagtg aacggcaggt atatgtgatg ggttaaaaag   4260

gatcggcggc cgctcgattt aaatc                                         4285


<210>  23
<211>  7074
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_ldhA

<400>  23
tcgagaggcc tgacgtcggg cccggtacca cgcgtcatat gactagttcg gacctaggga     60

tgggtcagcc tgaacgaacc gcacttgtat gtaggtagtt ttgaccgccc gaatattcgt    120

tataccttgg tggaaaaatt caaaccgatg gagcaattat acaattttgt ggcggcgcaa    180

aaaggtaaaa gcggtatcgt ctattgcaac agccgtagca aagtggagcg cattgcggaa    240

gccctgaaga aaagaggcat ttccgcagcc gcttatcatg cgggcatgga gccgtcgcag    300

cgggaagcgg tgcaacaggc gtttcaacgg gataatattc aagtggtggt ggcgaccatt    360

gcttttggta tggggatcaa caaatctaat gtgcgttttg tggcgcattt tgatttatct    420

cgcagcattg aggcgtatta tcaggaaacc gggcgcgcgg ggcgggacga cctgccggcg    480

gaagcggtac tgttttacga gccggcggat tatgcctggt tgcataaaat tttattggaa    540

gagccggaaa gcccgcaacg ggatattaaa cggcataagc tggaagccat cggcgaattt    600

gccgaaagcc agacctgccg tcgtttagtg ctgttaaatt atttcggcga aaaccgccaa    660

acgccatgta ataactgtga tatctgcctc gatccgccga aaaaatatga cggattatta    720

gacgcgcaga aaatcctttc gaccatttat cgcaccgggc aacgtttcgg cacgcaatac    780

gtaatcggcg taatgcgcgg tttgcagaat cagaaaataa aagaaaatca acatgatgag    840

ttgaaagtct acggaattgg caaagataaa agcaaagaat actggcaatc ggtaattcgt    900

cagctgattc atttgggctt tgtgcaacaa atcatcagcg atttcggcat ggggaccaga    960

ttacagctca ccgaaagcgc gcgtcccgtg ctgcgcggcg aagtgtcttt ggaactggcc   1020

atgccgagat tatcttccat taccatggta caggctccgc aacgcaatgc ggtaaccaac   1080

tacgacaaag atttatttgc ccgcctgcgt ttcctgcgca aacagattgc cgacaaagaa   1140

aacattccgc cttatattgt gttcagtgac gcgaccttgc aggaaatgtc gttgtatcag   1200

ccgaccagca aagtggaaat gctgcaaatc aacggtgtcg gcgccatcaa atggcagcgc   1260

ttcggacagc cttttatggc gattattaaa gaacatcagg ctttgcgtaa agcgggtaag   1320

aatccgttgg aattgcaatc ttaaaatttt taactttttg accgcacttt taaggttagc   1380

aaattccaat aaaaagtgcg gtgggttttc gggaattttt aacgcgctga tttcctcgtc   1440

ttttcaattt yttcgyctcc atttgttcgg yggttgccgg atcctttctt gactgagatc   1500

cataagagag tagaatagcg ccgcttatat ttttaatagc gtacctaatc gggtacgctt   1560

tttttatgcg gaaaatccat atttttctac cgcacttttt ctttaaagat ttatacttaa   1620

gtctgtttga ttcaatttat ttggaggttt tatgcaacac attcaactgg ctcccgattt   1680

aacattcagt cgcttaattc aaggattctg gcggttaaaa agctggcgga aatcgccgca   1740

ggaattgctt acattcgtta agcaaggatt agaattaggc gttgatacgc tggatcatgc   1800

cgcttgttac ggggctttta cttccgaggc ggaattcgga cgggcgctgg cgctggataa   1860

atccttgcgc gcacagctta ctttggtgac caaatgcggg attttgtatc ctaatgaaga   1920

attacccgat ataaaatccc atcactatga caacagctac cgccatatta tgtggtcggc   1980

gcaacgttcc attgaaaaac tgcaatgcga ctatttagat gtattgctga ttcaccgwct   2040

ttctccctgt gcggatcccg aacaaatcgc gcgggctttt gatgaacttt atcaaaccgg   2100

raaagtacgt tatttcgggg tatctaacta tacgccggct aagttcgcca tgttgcaatc   2160

ttatgtgaat cagccgttaa tcactaatca aattgagatt tcgcctcttc atcgtcaggc   2220

ttttgatgac ggtaccctgg attttttact ggaaaaacgt attcaaccga tggcatggtc   2280

gccacttgcc ggcggtcgtt tattcaatca ggatgagaac agtcgggcgg tgcaaaaaac   2340

attactcgaa atcggtgaaa cgaaaggaga aacccgttta gatacattgg cttatgcctg   2400

gttattggcg catccggcaa aaattatgcc ggttatgggg tccggtaaaa ttgaacgggt   2460

aaaaagcgcg gcggatgcgt tacgaatttc cttcactgag gaagaatgga ttaaggttta   2520

tgttgccgca cagggacggg atattccgta acatcatccg tctaatcctg cgtatctggg   2580

gaaagatgcg tcatcgtaag aggtctataa tattcgtcgt tttgataagg gtgccatatc   2640

cggcacccgt taaaatcaca ttgcgttcgc aacaaaatta ttccttacga atagcattca   2700

cctcttttaa cagatgttga atatccgtat cggcaaaaat atcctctata tttgcggtta   2760

aacggcgccg ccagttagca tattgagtgc tggttcccgg aatattgacg ggttcggtca   2820

taccgagcca gtcttcaggt tggaatcccc atcgtcgaca tcgatgctct tctgcgttaa   2880

ttaacaattg ggatcctcta gactccatag gccgctttcc tggctttgct tccagatgta   2940

tgctctcctc cggagagtac cgtgacttta ttttcggcac aaatacaggg gtcgatggat   3000

aaatacggcg atagtttcct gacggatgat ccgtatgtac cggcggaaga caagctgcaa   3060

acctgtcaga tggagattga tttaatggcg gatgtgctga gagcaccgcc ccgtgaatcc   3120

gcagaactga tccgctatgt gtttgcggat gattggccgg aataaataaa gccgggctta   3180

atacagatta agcccgtata gggtattatt actgaatacc aaacagctta cggaggacgg   3240

aatgttaccc attgagacaa ccagactgcc ttctgattat taatattttt cactattaat   3300

cagaaggaat aaccatgaat tttacccgga ttgacctgaa tacctggaat cgcagggaac   3360

actttgccct ttatcgtcag cagattaaat gcggattcag cctgaccacc aaactcgata   3420

ttaccgcttt gcgtaccgca ctggcggaga caggttataa gttttatccg ctgatgattt   3480

acctgatctc ccgggctgtt aatcagtttc cggagttccg gatggcactg aaagacaatg   3540

aacttattta ctgggaccag tcagacccgg tctttactgt ctttcataaa gaaaccgaaa   3600

cattctctgc actgtcctgc cgttattttc cggatctcag tgagtttatg gcaggttata   3660

atgcggtaac ggcagaatat cagcatgata ccagattgtt tccgcaggga aatttaccgg   3720

agaatcacct gaatatatca tcattaccgt gggtgagttt tgacgggatt taacctgaac   3780

atcaccggaa atgatgatta ttttgccccg gtttttacga tggcaaagtt tcagcaggaa   3840

ggtgaccgcg tattattacc tgtttctgta caggttcatc atgcagtctg tgatggcttt   3900

catgcagcac ggtttattaa tacacttcag ctgatgtgtg ataacatact gaaataaatt   3960

aattaattct gtatttaagc caccgtatcc ggcaggaatg gtggcttttt ttttatattt   4020

taaccgtaat ctgtaatttc gtttcagact ggttcaggat gagctcgctt ggactcctgt   4080

tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc   4140

gccgggcgtt ttttattggt gagaatccaa gcactagcgg cgcgccggcc ggcccggtgt   4200

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg   4260

ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag   4320

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa   4380

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc   4440

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca   4500

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg   4560

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct   4620

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt   4680

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag   4740

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc   4800

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac   4860

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga   4920

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc   4980

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg   5040

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca   5100

aaaaggatct tcacctagat ccttttaaag gccggccgcg gccgccatcg gcattttctt   5160

ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc tttgacaaca   5220

gatgttttct tgcctttgat gttcagcagg aagctcggcg caaacgttga ttgtttgtct   5280

gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc tttcgcttga   5340

ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc catttttaac   5400

acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga aacataacca   5460

agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc gcgggagtca   5520

gtgaacaggt accatttgcc gttcatttta aagacgttcg cgcgttcaat ttcatctgtt   5580

actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc atcgtttagc   5640

tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc gctttgcaga   5700

agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc tttgttaaat   5760

aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa tactaagtat   5820

ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc gcctgagctg   5880

tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc accgtcaaag   5940

attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc tgatacgtta   6000

acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc agtgtagaat   6060

aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg tgtttggtct   6120

tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg gccagcgttt   6180

ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat cgatgtgtca   6240

tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg atagtttgcg   6300

acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa   6360

gagatatttt taattgtgga cgaatcaaat tcagaaactt gatatttttc atttttttgc   6420

tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta tgtttcctta   6480

tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg   6540

gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc   6600

tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt tgaagatggc   6660

aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg ccaaagtata   6720

cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa cccgcgcgat   6780

ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc tattttcctc   6840

ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag aactaaaaaa   6900

tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg ggcataaagt   6960

tgccttttta atcacaattc agaaaatatc ataatatctc atttcactaa ataatagtga   7020

acggcaggta tatgtgatgg gttaaaaagg atcggcggcc gctcgattta aatc         7074


<210>  24
<211>  7183
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_pflA

<400>  24
tcgagtcaat gcggatttga cttatgatgt ggcaaacaac cgatttccga ttattactac     60

acgtaaaagt tattggaaag cggcgattgc ggagtttctg ggttatatcc gcggctacga    120

taatgcggcg gatttccgta aattaggagc aaaaacctgg gatgccaacg ctaatgaaaa    180

tcaggtatgg ctgaataacc ctcatcgcaa aggcaccgac gacatggggc gcgtttacgg    240

cgtacagggc agagcctggc gtaagcctaa cggcgaaacc gttgatcaat tacgcaaaat    300

tgtcaacaat ttaagtcgcg gcattgatga tcgcggcgaa attctgacct ttttaaaccc    360

gggcgaattc gatctcggtt gtctgcgccc ttgtatgtac aatcacacgt tttctttgct    420

gggcgatacg ctttatttaa ccagttatca acgctcctgt gacgtacctt taggcttgaa    480

tttcaatcaa attcaagtat ttacattctt agctttaatg gcgcagatta ccggtaaaaa    540

agccggtcag gcatatcaca aaatcgtcaa tgcgcatatt tacgaagacc agctggaact    600

aatgcgcgac gtgcagttaa aacgcgaacc gttcccgtcg ccaaaactgg aaattaatcc    660

ggacattaaa acccttgaag atttagaaac ctgggtaacc atggatgatt tcaacgtcgt    720

tggttaccaa tgccacgaac cgataaaata tccgttctcg gtataaaccg acaaaagtgc    780

ggtcaaaaat ttaatatttt catctgttat agaaaatatt tttcaacata aaatctaggg    840

atgcctgttt ggcgtccgta aatacgcaga aaaatattaa atttttgacc gcactttttt    900

catctcaatt aacagcctga taattcttat ggatcaacaa attagctttg acgaaaaaat    960

gatgaatcga gctcttttcc ttgccgacaa ggcggaagct ttaggggaaa ttcccgtagg   1020

tgccgtattg gtggatgaac ggggcaatat cattggtgaa ggctggaacc tctctattgt   1080

gaactcggat cccaccgccc atgccgaaat tattgcgttg cgtaacgccg cgcagaaaat   1140

ccaaaattac cgcctgctca ataccacttt atacgtgact ttagaaccct gcaccatgtg   1200

cgccggcgcg attttacaca gccgaatcaa acgcttggta ttcggggcgt ccgattacaa   1260

aaccggtgcg gtgggttcca gatttcattt ttttgaggat tataaaatga atcatggggt   1320

tgagatcaca agcggtgtct tacaggatca atgcagtcag aagttaagcc gctttttcca   1380

aaagcgcagg gaacagaaaa aacaacaaaa agctaccgca cttttacaac acccccggct   1440

taactcctct gaaaaatagt gacaaaaaaa ccgtcataat gtttacgacg gtttttttat   1500

ttcttaatat gcccttaaat aatcaacaaa atatagcaag aagattatag caaagaattt   1560

cgtttttttc agagaatagt caaatcttcg caaaaaacta ccgcactttt atccgcttta   1620

atcaggggaa ttaaaacaaa aaaattccgc ctattgaggc ggaatttatt aagcaataag   1680

acaaactctc aattacattg attgtgtaaa cgtacgagtg atgacgtctt gttgttgctc   1740

tttagttaat gagttgaaac gaaccgcgta acctgaaaca cgaatggtta attgcgggta   1800

tttttccgga ttttccatcg cgtctaacaa catttcacgg ttaagaacgt taacattcaa   1860

gtgttgaccg ccttccactg tcgcttcatg atggaaataa ccgtccatta aaccggcaag   1920

gttgcgtttt tgcgcttcgt catctttacc taatgcgttc ggtacgatag agaaggtata   1980

tgaaataccg tctttcgcgt aagcgaacgg aagtttagcc acagaagtaa gtgaagcaac   2040

cgcacctttt tggtcacgac cgtgcattgg gtttgcaccc ggtccgaatg gcgcgcctgc   2100

tcgacgaccg tccggagtat taccggtttt cttaccgtat accacgttag aagtgatagt   2160

caggatagat tgtgtcggag ttgcgttgcg gtaagttttg tgtttttgaa cttttttcat   2220

gaaacgttca actaagtcta ccgctaaatc atcaacacgc ggatcattgt taccgaattg   2280

cggatattcg ccttcaattt cgaagtcgat agcaacattc gaggccacga cattaccgtc   2340

tttatctttg atgtcgccgc gaatcggttt aactttcgca tatttgattg cggataatga   2400

gtccgcagcc acggaaagac ccgcgatacc gcaagccatt gtacggaata cgtcgcgatc   2460

gtggaacgcc atcaatgccg cttcatatgc atatttatcg tgcatgaagt ggatgatgtt   2520

caatgcggtt acatattgag tcgccaacca gtccatgaaa ctgtccatac gttcgattac   2580

ggtatcgaaa ttcaatactt cgtctgtaat cggcgcagtt ttaggaccga cttgcatacc   2640

atttttctca tcgataccgc cgttaattgc gtataacata gttttagcta agtttgcgcg   2700

cgcaccgaag aattgcattt gtttacctac gaccatcggt gatacgcagc atgcgattgc   2760

atagtcatcg ttgttgaagt caggacgcat taagtcatca ttttcgtatt gtacggagga   2820

agtatcaata gatactttcg cacagaaacg tttgaacgct tcaggtaatt gttcggacca   2880

aagaatagtt aagtttggtt ccggagaagt acccatagtg tataaagtat gtaatacgcg   2940

gaagctgttt ttagttacca acggacgacc gtctaagccc ataccggcga tagtttcggt   3000

tgccctctag actccatagg ccgctttcct ggctttgctt ccagatgtat gctctcctcc   3060

ggagagtacc gtgactttat tttcggcaca aatacagggg tcgatggata aatacggcga   3120

tagtttcctg acggatgatc cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat   3180

ggagattgat ttaatggcgg atgtgctgag agcaccgccc cgtgaatccg cagaactgat   3240

ccgctatgtg tttgcggatg attggccgga ataaataaag ccgggcttaa tacagattaa   3300

gcccgtatag ggtattatta ctgaatacca aacagcttac ggaggacgga atgttaccca   3360

ttgagacaac cagactgcct tctgattatt aatatttttc actattaatc agaaggaata   3420

accatgaatt ttacccggat tgacctgaat acctggaatc gcagggaaca ctttgccctt   3480

tatcgtcagc agattaaatg cggattcagc ctgaccacca aactcgatat taccgctttg   3540

cgtaccgcac tggcggagac aggttataag ttttatccgc tgatgattta cctgatctcc   3600

cgggctgtta atcagtttcc ggagttccgg atggcactga aagacaatga acttatttac   3660

tgggaccagt cagacccggt ctttactgtc tttcataaag aaaccgaaac attctctgca   3720

ctgtcctgcc gttattttcc ggatctcagt gagtttatgg caggttataa tgcggtaacg   3780

gcagaatatc agcatgatac cagattgttt ccgcagggaa atttaccgga gaatcacctg   3840

aatatatcat cattaccgtg ggtgagtttt gacgggattt aacctgaaca tcaccggaaa   3900

tgatgattat tttgccccgg tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt   3960

attattacct gtttctgtac aggttcatca tgcagtctgt gatggctttc atgcagcacg   4020

gtttattaat acacttcagc tgatgtgtga taacatactg aaataaatta attaattctg   4080

tatttaagcc accgtatccg gcaggaatgg tggctttttt tttatatttt aaccgtaatc   4140

tgtaatttcg tttcagactg gttcaggatg agctcgcttg gactcctgtt gatagatcca   4200

gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt   4260

tttattggtg agaatccaag cactagcggc gcgccggccg gcccggtgtg aaataccgca   4320

cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc   4380

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg   4440

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa   4500

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga   4560

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag   4620

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct   4680

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg   4740

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc   4800

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt   4860

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta   4920

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac   4980

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc   5040

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat   5100

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc   5160

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt   5220

cacctagatc cttttaaagg ccggccgcgg ccgccatcgg cattttcttt tgcgttttta   5280

tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt   5340

gcctttgatg ttcagcagga agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc   5400

tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa   5460

gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt   5520

tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat   5580

atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta   5640

ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga   5700

tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc   5760

gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact   5820

ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc   5880

gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt   5940

atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc   6000

atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata   6060

atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt   6120

tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt   6180

tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga   6240

atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc   6300

aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt   6360

aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc   6420

agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt   6480

aattgtggac gaatcaaatt cagaaacttg atatttttca tttttttgct gttcagggat   6540

ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg   6600

gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt   6660

taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat   6720

gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac   6780

gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct   6840

ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga   6900

cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat   6960

agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt   7020

cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa   7080

tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat   7140

atgtgatggg ttaaaaagga tcggcggccg ctcgatttaa atc                     7183


<210>  25
<211>  7557
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_wcaJ*

<400>  25
tcgagtaagc cgattcagct gatccgccac atggggaaaa agcctaatct gcggaatatg     60

aaaccgatac cagtccagta aagttgacaa atcgacatca tattgctcaa ccaagtattg    120

aaaagcgttt tcaccgcgat gatacaattc gaccagccgg ttaaataacg tttcactccg    180

ttccggtgcc aaacgagacg caatatgctt ataggcggaa tacagaaaat cgatttccgc    240

ataaagcgta tcgtccaaat ctaaaaccaa cgctttattt ttcataatga tgagccagta    300

cttccgcgtc ataccgcaac attaataaat ccgcttccca gtcttcgaac gcaggaatcg    360

gctgattaaa caaatattcc tgaatcaacc aacggggata attggctccc gccagataac    420

tcaacggata accgccgccg aacctagggt taatttcaat accaagaatt tcagcggtgg    480

attccttata aaatacttgg attgttaagc aaccgcgcgc ccccggtaaa cgggacaatt    540

tttccgataa ttgcgtcacg atggcatttt ttctggtcac acctttgtta atttcccccg    600

ctctgacaaa aattctcttt ctcggtaccg cacttttcag ttcggaattt ttatcaaaat    660

aacaatccac ggtatattcg tcgtattccg ccggcgaaat atattgcata aacattaatt    720

cgggattttc caattgctcc ggtgaaatat cttccggttt ctccgccaca aaaattcctt    780

tacttaaact accgttgtaa ggcttcacaa aaacaggata ttcaaattga cctttttcaa    840

actgcttcgg taccgcaata ttatgttcaa taaacagttg attggttaat cgtttgtcgc    900

gacattttct gacaaactct gtatcactaa cggaaataaa aatacctttt tctttaaacc    960

gttgcagatg ttcgcttaaa ataagcaatt ccgtatcaat agtcggaata atcaatttca   1020

cgttattttc ttcacagatt ttaagtaagg tcggaatata ctccgcatca gtgacccggg   1080

gtacaggaaa atgtccgtcg gccacataac aagccggcgc caactcggga tttaaatcta   1140

cggttaacac ttttccgtca cttactaact gcgataattc ctttttaaac gcctgaacga   1200

gagaaacacg ttgtccggcc gatgtaacaa gaatattcat gattttttat tcccttcaaa   1260

tttaggcatg gtggcatcat ctgccgcatt aatatcgtct tttgcgatca ctttttggat   1320

ggtctttgcg ataattttca aatccagcca caaggattga tgttcaacat accaggcatc   1380

caattcaaat ttctgctccc aactgatggc attgcgaccg tttacctgtg cataaccggt   1440

aattccgggt ttcacttcat ggcgcttagc ctgtctttcg ttatacagcg gcaaatattc   1500

catcagtaga ggacgcggcc ccaccagact catatcacct tttaatacat tccaaagttc   1560

cggcaactcg tccagactgg tagcgcgcaa cattttgccg aaaggtgtta agcgctccgc   1620

atccggcaaa atattaccgt tttcatctgc gccgtctttc attgttctga acttaatcat   1680

ctcaaagggt ttaccatgca atccggggcg ggtttgttta aataacaccg gtgatcccaa   1740

attttgtttt accttgataa gccacaaaca aatataaggg cgaaaacaaa atcaatgcta   1800

tcaatgcgac aacaatatcg aaaaggcgtt ttatcatgaa aatctcctac gaccgaccaa   1860

tttggggctg acaaaagtgc cgtttttcac cagaaccgta taaactaaaa ccaggaaaag   1920

cggataccag actaacggca gtcttaatat ggaagacggc acccaaacga tccaacaaaa   1980

attaataaaa ataaataaaa taaatatacg ttctttccac gccaacaatt gggtattcat   2040

cacatagata attgaattta ataaaaaata tataaatgcc aaaaatgcaa aaatgccaaa   2100

tgccaaataa agttctataa agaagctatg cggattagtg taacccaaag ggaaacttaa   2160

ctttatttga tcgaaatact gaatgtagtc ccgcggtcca taacccaacc ataaaatttt   2220

aaaattatct aaaaatgtcg tataaatttc cgtccggtaa cctacggact tatcatcgcc   2280

catagaaaat attaccaatg aaaaacgttc aatcggacgc tccagccaat caattttggc   2340

gagcagaata aacacttcct gtaaccaaga aagattaaat ataaataatg cgatcacgca   2400

ggcgaaaaac agatataccg ccttaaaata ggtagatgcg tttaaaaaca agatcagcat   2460

caacataatc aaatagctca gtaataccga acgggaggca ctgatcacaa tagctaaccc   2520

cataataaaa ataagagcat agccgattaa cttaattttc cagttgtttt ctctgatgat   2580

gtaaaaaaat cccaccgcca ccgcaagaga aatcataatt acggactggt cattggtatt   2640

aaagaaaaaa cctttaaacg ccttatcagt tacggttaat tcttcattac ccgaaaccaa   2700

ctggaacccc aataaagcct caataaaaaa gcccgccagc acaattaatg atattcccaa   2760

caaaaggtgc ctaatccctg cttctccatc accccggtta aacgtcaaaa aagaataatg   2820

aaataaaaac atcacaattc cgaagaaaaa caaatcaact aatttttctg tagaaaacgc   2880

atttaatacc gataaaaagc cgaagaaaaa caacacgtac accggaaact gtaatttaaa   2940

aaaatcagtt tccatatccc ttaaaaaagg ggttattacc gctaagaaaa agaacaaaaa   3000

acacaacgca ctatctaacc tcggcactcc tatttgtgtc gatagtgcgg gagaaagtat   3060

caccagtccc aacgcaaaga gtaatagcaa cttaaaaatg ctgataacat taatattcat   3120

atcaaataat atttttgatt aatttctcaa tttctttata agaacgctcg cgcagaaact   3180

tctcttttgc cagcgataaa ttcacttgcg acattttgtc taaaaccgtt ctgtcttcgg   3240

ccaatttatt caacgtctca gccaactccc gataatctcc cgccgtatat tgaattccac   3300

cgcctttcgc cagtagtttt tccacttcag gatgtttctg acagcttaca atcggtaatg   3360

cgcaacagat ataatcggat ctagactcca taggccgctt tcctggcttt gcttccagat   3420

gtatgctctc ctccggagag taccgtgact ttattttcgg cacaaataca ggggtcgatg   3480

gataaatacg gcgatagttt cctgacggat gatccgtatg taccggcgga agacaagctg   3540

caaacctgtc agatggagat tgatttaatg gcggatgtgc tgagagcacc gccccgtgaa   3600

tccgcagaac tgatccgcta tgtgtttgcg gatgattggc cggaataaat aaagccgggc   3660

ttaatacaga ttaagcccgt atagggtatt attactgaat accaaacagc ttacggagga   3720

cggaatgtta cccattgaga caaccagact gccttctgat tattaatatt tttcactatt   3780

aatcagaagg aataaccatg aattttaccc ggattgacct gaatacctgg aatcgcaggg   3840

aacactttgc cctttatcgt cagcagatta aatgcggatt cagcctgacc accaaactcg   3900

atattaccgc tttgcgtacc gcactggcgg agacaggtta taagttttat ccgctgatga   3960

tttacctgat ctcccgggct gttaatcagt ttccggagtt ccggatggca ctgaaagaca   4020

atgaacttat ttactgggac cagtcagacc cggtctttac tgtctttcat aaagaaaccg   4080

aaacattctc tgcactgtcc tgccgttatt ttccggatct cagtgagttt atggcaggtt   4140

ataatgcggt aacggcagaa tatcagcatg ataccagatt gtttccgcag ggaaatttac   4200

cggagaatca cctgaatata tcatcattac cgtgggtgag ttttgacggg atttaacctg   4260

aacatcaccg gaaatgatga ttattttgcc ccggttttta cgatggcaaa gtttcagcag   4320

gaaggtgacc gcgtattatt acctgtttct gtacaggttc atcatgcagt ctgtgatggc   4380

tttcatgcag cacggtttat taatacactt cagctgatgt gtgataacat actgaaataa   4440

attaattaat tctgtattta agccaccgta tccggcagga atggtggctt tttttttata   4500

ttttaaccgt aatctgtaat ttcgtttcag actggttcag gatgagctcg cttggactcc   4560

tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt   4620

gccgccgggc gttttttatt ggtgagaatc caagcactag cggcgcgccg gccggcccgg   4680

tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc   4740

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca   4800

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca   4860

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg   4920

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg   4980

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt   5040

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt   5100

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc   5160

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt   5220

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt   5280

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc   5340

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa   5400

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt   5460

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct   5520

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta   5580

tcaaaaagga tcttcaccta gatcctttta aaggccggcc gcggccgcca tcggcatttt   5640

cttttgcgtt tttatttgtt aactgttaat tgtccttgtt caaggatgct gtctttgaca   5700

acagatgttt tcttgccttt gatgttcagc aggaagctcg gcgcaaacgt tgattgtttg   5760

tctgcgtaga atcctctgtt tgtcatatag cttgtaatca cgacattgtt tcctttcgct   5820

tgaggtacag cgaagtgtga gtaagtaaag gttacatcgt taggatcaag atccattttt   5880

aacacaaggc cagttttgtt cagcggcttg tatgggccag ttaaagaatt agaaacataa   5940

ccaagcatgt aaatatcgtt agacgtaatg ccgtcaatcg tcatttttga tccgcgggag   6000

tcagtgaaca ggtaccattt gccgttcatt ttaaagacgt tcgcgcgttc aatttcatct   6060

gttactgtgt tagatgcaat cagcggtttc atcacttttt tcagtgtgta atcatcgttt   6120

agctcaatca taccgagagc gccgtttgct aactcagccg tgcgtttttt atcgctttgc   6180

agaagttttt gactttcttg acggaagaat gatgtgcttt tgccatagta tgctttgtta   6240

aataaagatt cttcgccttg gtagccatct tcagttccag tgtttgcttc aaatactaag   6300

tatttgtggc ctttatcttc tacgtagtga ggatctctca gcgtatggtt gtcgcctgag   6360

ctgtagttgc cttcatcgat gaactgctgt acattttgat acgtttttcc gtcaccgtca   6420

aagattgatt tataatcctc tacaccgttg atgttcaaag agctgtctga tgctgatacg   6480

ttaacttgtg cagttgtcag tgtttgtttg ccgtaatgtt taccggagaa atcagtgtag   6540

aataaacgga tttttccgtc agatgtaaat gtggctgaac ctgaccattc ttgtgtttgg   6600

tcttttagga tagaatcatt tgcatcgaat ttgtcgctgt ctttaaagac gcggccagcg   6660

tttttccagc tgtcaataga agtttcgccg actttttgat agaacatgta aatcgatgtg   6720

tcatccgcat ttttaggatc tccggctaat gcaaagacga tgtggtagcc gtgatagttt   6780

gcgacagtgc cgtcagcgtt ttgtaatggc cagctgtccc aaacgtccag gccttttgca   6840

gaagagatat ttttaattgt ggacgaatca aattcagaaa cttgatattt ttcatttttt   6900

tgctgttcag ggatttgcag catatcatgg cgtgtaatat gggaaatgcc gtatgtttcc   6960

ttatatggct tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc tgccagcagt   7020

gcggtagtaa aggttaatac tgttgcttgt tttgcaaact ttttgatgtt catcgttcat   7080

gtctcctttt ttatgtactg tgttagcggt ctgcttcttc cagccctcct gtttgaagat   7140

ggcaagttag ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg acgccaaagt   7200

atacactttg ccctttacac attttaggtc ttgcctgctt tatcagtaac aaacccgcgc   7260

gatttacttt tcgacctcat tctattagac tctcgtttgg attgcaactg gtctattttc   7320

ctcttttgtt tgatagaaaa tcataaaagg atttgcagac tacgggccta aagaactaaa   7380

aaatctatct gtttcttttc attctctgta ttttttatag tttctgttgc atgggcataa   7440

agttgccttt ttaatcacaa ttcagaaaat atcataatat ctcatttcac taaataatag   7500

tgaacggcag gtatatgtga tgggttaaaa aggatcggcg gccgctcgat ttaaatc      7557


<210>  26
<211>  8693
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_delta_ptsG_cscA

<400>  26
ctagactcca taggccgctt tcctggcttt gcttccagat gtatgctctc ctccggagag     60

taccgtgact ttattttcgg cacaaataca ggggtcgatg gataaatacg gcgatagttt    120

cctgacggat gatccgtatg taccggcgga agacaagctg caaacctgtc agatggagat    180

tgatttaatg gcggatgtgc tgagagcacc gccccgtgaa tccgcagaac tgatccgcta    240

tgtgtttgcg gatgattggc cggaataaat aaagccgggc ttaatacaga ttaagcccgt    300

atagggtatt attactgaat accaaacagc ttacggagga cggaatgtta cccattgaga    360

caaccagact gccttctgat tattaatatt tttcactatt aatcagaagg aataaccatg    420

aattttaccc ggattgacct gaatacctgg aatcgcaggg aacactttgc cctttatcgt    480

cagcagatta aatgcggatt cagcctgacc accaaactcg atattaccgc tttgcgtacc    540

gcactggcgg agacaggtta taagttttat ccgctgatga tttacctgat ctcccgggct    600

gttaatcagt ttccggagtt ccggatggca ctgaaagaca atgaacttat ttactgggac    660

cagtcagacc cggtctttac tgtctttcat aaagaaaccg aaacattctc tgcactgtcc    720

tgccgttatt ttccggatct cagtgagttt atggcaggtt ataatgcggt aacggcagaa    780

tatcagcatg ataccagatt gtttccgcag ggaaatttac cggagaatca cctgaatata    840

tcatcattac cgtgggtgag ttttgacggg atttaacctg aacatcaccg gaaatgatga    900

ttattttgcc ccggttttta cgatggcaaa gtttcagcag gaaggtgacc gcgtattatt    960

acctgtttct gtacaggttc atcatgcagt ctgtgatggc tttcatgcag cacggtttat   1020

taatacactt cagctgatgt gtgataacat actgaaataa attaattaat tctgtattta   1080

agccaccgta tccggcagga atggtggctt tttttttata ttttaaccgt aatctgtaat   1140

ttcgtttcag actggttcag gatgagctcg cttggactcc tgttgataga tccagtaatg   1200

acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt   1260

ggtgagaatc caagcactag cggcgcgccg gccggcccgg tgtgaaatac cgcacagatg   1320

cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg   1380

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc   1440

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag   1500

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca   1560

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca   1620

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg   1680

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag   1740

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt   1800

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca   1860

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg   1920

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt   1980

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc   2040

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg   2100

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg   2160

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta   2220

gatcctttta aaggccggcc gcggccgcca tcggcatttt cttttgcgtt tttatttgtt   2280

aactgttaat tgtccttgtt caaggatgct gtctttgaca acagatgttt tcttgccttt   2340

gatgttcagc aggaagctcg gcgcaaacgt tgattgtttg tctgcgtaga atcctctgtt   2400

tgtcatatag cttgtaatca cgacattgtt tcctttcgct tgaggtacag cgaagtgtga   2460

gtaagtaaag gttacatcgt taggatcaag atccattttt aacacaaggc cagttttgtt   2520

cagcggcttg tatgggccag ttaaagaatt agaaacataa ccaagcatgt aaatatcgtt   2580

agacgtaatg ccgtcaatcg tcatttttga tccgcgggag tcagtgaaca ggtaccattt   2640

gccgttcatt ttaaagacgt tcgcgcgttc aatttcatct gttactgtgt tagatgcaat   2700

cagcggtttc atcacttttt tcagtgtgta atcatcgttt agctcaatca taccgagagc   2760

gccgtttgct aactcagccg tgcgtttttt atcgctttgc agaagttttt gactttcttg   2820

acggaagaat gatgtgcttt tgccatagta tgctttgtta aataaagatt cttcgccttg   2880

gtagccatct tcagttccag tgtttgcttc aaatactaag tatttgtggc ctttatcttc   2940

tacgtagtga ggatctctca gcgtatggtt gtcgcctgag ctgtagttgc cttcatcgat   3000

gaactgctgt acattttgat acgtttttcc gtcaccgtca aagattgatt tataatcctc   3060

tacaccgttg atgttcaaag agctgtctga tgctgatacg ttaacttgtg cagttgtcag   3120

tgtttgtttg ccgtaatgtt taccggagaa atcagtgtag aataaacgga tttttccgtc   3180

agatgtaaat gtggctgaac ctgaccattc ttgtgtttgg tcttttagga tagaatcatt   3240

tgcatcgaat ttgtcgctgt ctttaaagac gcggccagcg tttttccagc tgtcaataga   3300

agtttcgccg actttttgat agaacatgta aatcgatgtg tcatccgcat ttttaggatc   3360

tccggctaat gcaaagacga tgtggtagcc gtgatagttt gcgacagtgc cgtcagcgtt   3420

ttgtaatggc cagctgtccc aaacgtccag gccttttgca gaagagatat ttttaattgt   3480

ggacgaatca aattcagaaa cttgatattt ttcatttttt tgctgttcag ggatttgcag   3540

catatcatgg cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct tttggttcgt   3600

ttctttcgca aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa aggttaatac   3660

tgttgcttgt tttgcaaact ttttgatgtt catcgttcat gtctcctttt ttatgtactg   3720

tgttagcggt ctgcttcttc cagccctcct gtttgaagat ggcaagttag ttacgcacaa   3780

taaaaaaaga cctaaaatat gtaaggggtg acgccaaagt atacactttg ccctttacac   3840

attttaggtc ttgcctgctt tatcagtaac aaacccgcgc gatttacttt tcgacctcat   3900

tctattagac tctcgtttgg attgcaactg gtctattttc ctcttttgtt tgatagaaaa   3960

tcataaaagg atttgcagac tacgggccta aagaactaaa aaatctatct gtttcttttc   4020

attctctgta ttttttatag tttctgttgc atgggcataa agttgccttt ttaatcacaa   4080

ttcagaaaat atcataatat ctcatttcac taaataatag tgaacggcag gtatatgtga   4140

tgggttaaaa aggatcggcg gccgctcgat ttaaatctcg agggataaaa ctatgagcca   4200

atatactcgc aaaacattcg atgaagtgat gatccaaaac tacgtacctg cggattttat   4260

tcctgtgaaa ggcaaaggtt gcaaagtttg ggatcagcaa gggcgagatt atattgattt   4320

taccagcgga attgccgtaa atgcattggg acattgtcct gacgaaatcg tcgatgtgct   4380

gaaaaaacaa ggcgaaactc tatggcattc aagcaactgg ttcaccagtg aaccgactct   4440

ggaacttgcc tccaaattgg ttgaacatac ctttgccgaa cgtgtgatgt ttgccaattc   4500

cggcggtgaa gccaatgagg cggcgcttaa attggcgcgt cgttatgcgg tggataatta   4560

cggctatcaa aaagatacca ttatttcatt caaaaaaagt ttccacggac gtaccttatt   4620

taccgtcagc gtcggcggtc aggcgaaata ttcggacgga ttcggcccga aacctgccgg   4680

aatcgtgcat ttgccgttta atgatcttga tgcggttaaa gcgatgattg atgatcacag   4740

ctgtgcggta atcgttgagc cgattcaggg agaaagcggg attattccgg ctaccaaaga   4800

atttttacag ggtttgcgtc gactttgcga cgaaaataac gctctattga tttttgacga   4860

agtacaaacg ggggtagggc gtaccggtta tttatacgca tatgaaagtt atgatgtagt   4920

gccggatatt ctgacctcgt caaaagccct tgctaacggt tttccgatta gcgcaatgtt   4980

aaccaccaca aaaattgccg ccagtttcaa accgggcgtg cacggcacca cattcggcgg   5040

taatccgtta gcctgtgcgg tcggggcgaa agtgattgag acgattgcaa atccggcgtt   5100

tcttgaaaat gtacaaaaaa catccgcact ttttatcagc gaattaaaca aacttaatga   5160

gaaatatcac ttatttaacg aagttcgcgg tcagggatta ttaatcgggg cggaattaat   5220

tgaaaaatat cagggcaaag cctcagagtt tgtcaaagcc tgtgcggata atcaacttat   5280

gattttagtc gctgggccga atgtgctacg ttttgcgccg gcattaaata ttagtcaaca   5340

ggaagtggcg gaaggattta aacgtttaga tcaggctctg caaaaatttg cttaaaattg   5400

tgaagaaaag caaattattt aaaaattcaa tattgacata aatagtaaat aactttacac   5460

ttcttgacgt tttatctaag agtgactttg atagagctaa actttaaata ttttattcat   5520

aaaacccttt tagccagagt atttttgctc tggctctttt ctttttaaac ttccgtttct   5580

tttgaatttg atctaaatct aggaaattta cctaattttt agatttttct agtgttgctt   5640

gctcgtttta gctagaattg gcgaaaattt ttgcttaatt tataaacgag gagtcgctat   5700

gacgcaatct cgattgcatg cggcgcaaaa cgccctagca aaacttcatg agcaccgggg   5760

taacactttc tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa   5820

cggcctgatc tggtttaacg atcgttatca cgcgttttat caacatcatc cgatgagcga   5880

acactggggg ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca   5940

tgagcctatt gcgctagcgc caggagacga taatgacaaa gacgggtgtt tttcaggtag   6000

tgctgtcgat gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg   6060

tgcaggtaat gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat   6120

tcatttcgag aaacagggtg tgatcctcac tccaccagaa ggaatcatgc acttccgcga   6180

tcctaaagtg tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagatccagg   6240

caacacgggg cagatcctgc tttatcgcgg cagttcgttg cgtgaatgga ccttcgatcg   6300

cgtactggcc cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag   6360

ccttggcgat cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag   6420

ttaccgaaat cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt   6480

tgcacaatcc gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag   6540

ctttttagcg aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc   6600

aatgccctca aaacgtgaag gatgggcagg ctgcatgacg ctggcgcgcg agctatcaga   6660

gagcaatggc aaacttctac aacgcccggt acacgaagct gagtcgttac gccagcagca   6720

tcaatctgtc tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc   6780

agttgagatt cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca   6840

gctcggcact ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg   6900

gtattaccca cacgagaatt tagacggcta ccgtagtatt cccctcccgc agcgtgacac   6960

gctcgcccta aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga   7020

agcggtgatg agtagtcgaa tctatccgca gccagaagaa cgggaactgt cgctttatgc   7080

ctcccacgga gtggctgtgc tgcaacatgg agcactctgg ctactgggtt aacataatat   7140

caggtggaac aacggatcaa cagcgggcaa gggatccgtt taaaaaatca gtcaaaaaag   7200

accgcacttt taacgcaaag ttcggtcttt tttttgctat tttttctccg gtttttccgt   7260

atcggctatt tttaaccggt aggaaaatat taatcccaac aggcttaaaa cggataaggt   7320

ctgcaaggcg atttgcagac cgtaatgatc tgcaatccag cccacaattg gtgaaaatat   7380

cccgcccata gttattccca gcccaagggt aatgccggct gcaaagccga cgcttttggc   7440

aagataagcc tgaccaagca cgacaatagg actatattga gtaaaaacgc ccagtcccaa   7500

cggaattagc aaaataaatg agagccataa gttttcgctt tgagtaaaaa ttaaaatagt   7560

gggtaaaaaa atcaaataag cgtagcggat aattctgaca taacccaggc gatcggaaag   7620

caacccgccg ataaaggtaa tggcgacccc cattgataaa aatattgtga gcgcaaagtt   7680

agcgtcggtt tcttgttgat gaagtatatg aatccaatag ataggaataa aagcgttaag   7740

tactgtgaaa ttggtggcgc ggacaaaaat aatcaccgac aatttggcaa aactgcgcca   7800

atcgttttgt aatgtggtag aagcggtgtt ttttgcttta ttaaccacat tttcgactgt   7860

taattttggc aactgtaaga aaataatgag cgcgatgatc gtgttaatca gggcaaaaat   7920

acttaaggtt tgcgcaccga ataaataggc gagccccgcg aacatcggtc ctatagcaaa   7980

accggcattg ccgccgacgg caaaaatccc catagctttg cctttttctc cgccggacat   8040

tcggttaacc agtctcgctc cttccgggtg aaacagagcg gaacctatgc cggcaatcat   8100

ggcaaaaaag aataaacccg ggtaggaatg gacaaagccc atagcggcaa tacagcagcc   8160

tgacagcatc attcccaaag gaattaacca aggcatagaa cgtttatccg ccaaatagcc   8220

gaaaaacggt tgagcaattg aggcgagaac cgtgttggcg aaaattaatc cgccggcttc   8280

ctgataggtc aatccgtaat tttttataaa aagcggaagc agggcgggga gcgctccttg   8340

cgccatatcg gtgacgagat gcccgattgc cgcaagataa acagcgaatt tgttttgcat   8400

aataagttcg ttaaaagata aataagttct attataatcc cgaagtttcc ggtgtatttt   8460

aataaaacga attattttgc attttattac ttaagatcaa aagacgggta tcgggaaatt   8520

aaaagtgcgg tgaaaatttg cgtttttttg gcgggtagaa aaaagccctt tcatcatgaa   8580

agggcaaaat acatttggag ttatattttt taaagtatgt ttgtattata tatgcggaat   8640

tttaactttt acaacatatt tttaacattt acttttggtg aggattgcgc gtt          8693


<210>  27
<211>  8100
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid 
       pSacB_delta_ptsG_argD::cscA-fusion

<400>  27
tgtttccgca gggaaattta ccggagaatc acctgaatat atcatcatta ccgtgggtga     60

gttttgacgg gatttaacct gaacatcacc ggaaatgatg attattttgc cccggttttt    120

acgatggcaa agtttcagca ggaaggtgac cgcgtattat tacctgtttc tgtacaggtt    180

catcatgcag tctgtgatgg ctttcatgca gcacggttta ttaatacact tcagctgatg    240

tgtgataaca tactgaaata aattaattaa ttctgtattt aagccaccgt atccggcagg    300

aatggtggct ttttttttat attttaaccg taatctgtaa tttcgtttca gactggttca    360

ggatgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg    420

atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcacta    480

gcggcgcgcc ggccggcccg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc    540

atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg    600

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac    660

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg    720

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca    780

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc    840

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc    900

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag    960

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc   1020

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca   1080

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg   1140

aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg   1200

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct   1260

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa   1320

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa   1380

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaaggccggc   1440

cgcggccgcc atcggcattt tcttttgcgt ttttatttgt taactgttaa ttgtccttgt   1500

tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt tgatgttcag caggaagctc   1560

ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt ttgtcatata gcttgtaatc   1620

acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg agtaagtaaa ggttacatcg   1680

ttaggatcaa gatccatttt taacacaagg ccagttttgt tcagcggctt gtatgggcca   1740

gttaaagaat tagaaacata accaagcatg taaatatcgt tagacgtaat gccgtcaatc   1800

gtcatttttg atccgcggga gtcagtgaac aggtaccatt tgccgttcat tttaaagacg   1860

ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa tcagcggttt catcactttt   1920

ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag cgccgtttgc taactcagcc   1980

gtgcgttttt tatcgctttg cagaagtttt tgactttctt gacggaagaa tgatgtgctt   2040

ttgccatagt atgctttgtt aaataaagat tcttcgcctt ggtagccatc ttcagttcca   2100

gtgtttgctt caaatactaa gtatttgtgg cctttatctt ctacgtagtg aggatctctc   2160

agcgtatggt tgtcgcctga gctgtagttg ccttcatcga tgaactgctg tacattttga   2220

tacgtttttc cgtcaccgtc aaagattgat ttataatcct ctacaccgtt gatgttcaaa   2280

gagctgtctg atgctgatac gttaacttgt gcagttgtca gtgtttgttt gccgtaatgt   2340

ttaccggaga aatcagtgta gaataaacgg atttttccgt cagatgtaaa tgtggctgaa   2400

cctgaccatt cttgtgtttg gtcttttagg atagaatcat ttgcatcgaa tttgtcgctg   2460

tctttaaaga cgcggccagc gtttttccag ctgtcaatag aagtttcgcc gactttttga   2520

tagaacatgt aaatcgatgt gtcatccgca tttttaggat ctccggctaa tgcaaagacg   2580

atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt tttgtaatgg ccagctgtcc   2640

caaacgtcca ggccttttgc agaagagata tttttaattg tggacgaatc aaattcagaa   2700

acttgatatt tttcattttt ttgctgttca gggatttgca gcatatcatg gcgtgtaata   2760

tgggaaatgc cgtatgtttc cttatatggc ttttggttcg tttctttcgc aaacgcttga   2820

gttgcgcctc ctgccagcag tgcggtagta aaggttaata ctgttgcttg ttttgcaaac   2880

tttttgatgt tcatcgttca tgtctccttt tttatgtact gtgttagcgg tctgcttctt   2940

ccagccctcc tgtttgaaga tggcaagtta gttacgcaca ataaaaaaag acctaaaata   3000

tgtaaggggt gacgccaaag tatacacttt gccctttaca cattttaggt cttgcctgct   3060

ttatcagtaa caaacccgcg cgatttactt ttcgacctca ttctattaga ctctcgtttg   3120

gattgcaact ggtctatttt cctcttttgt ttgatagaaa atcataaaag gatttgcaga   3180

ctacgggcct aaagaactaa aaaatctatc tgtttctttt cattctctgt attttttata   3240

gtttctgttg catgggcata aagttgcctt tttaatcaca attcagaaaa tatcataata   3300

tctcatttca ctaaataata gtgaacggca ggtatatgtg atgggttaaa aaggatcggc   3360

ggccgctcga tttaaatctc gagtaaaatg aatgcccgcc tgatggatgt agcgccgacc   3420

ggaaacgggc gccgcgaatc ttatgcgaat ttgccgatgc cccgcatgac caatacttat   3480

atgttgtcgg gcgacagcaa gtttgaggat ttgatcggtt caattgatag aggtattttt   3540

gcctcgcatt tcggcggcgg gcaagtggat attacttccg gaaaattcac tttctccacc   3600

acagaagcct accttattga aaaggggaaa atcactcgtc cggtaaaagg cgctacttta   3660

atcggcagcg gtattgaagt gatgcagcag gtttcaatgg tggcggataa tatggaaatc   3720

gaccatggta tcggcgtgtg cggtaaagag ggacaaagtg tgccggtcgg cgtgggacaa   3780

cccgcattaa aaatcgaaaa aatcaccgtt ggcggtacaa attaaacaat aacgaagacc   3840

gtacataaaa gtgcggtctt ttttatgtga atttttattc attatatttg actaattatt   3900

caataagttc tatgctaaat ccaatttaac acaacataag aaagaaaagg ataaaactat   3960

gagccaatat actcgcaaaa cattcgatga agtgatgatc caaaactacg tacctgcgga   4020

ttttattcct gtgaaaggca aaggttgcaa agtttgggat cagcaagggc gagattatat   4080

tgattttacc agcggaattg ccgtaaatgc attgggacat tgtcctgacg aaatcgtcga   4140

tgtgctgaaa aaacaaggcg aaactctatg gcattcaagc aactggttca ccagtgaacc   4200

gactctggaa cttgcctcca aattggttga acataccttt gccgaacgtg tgatgtttgc   4260

caattccggc ggtgaagcca atgaggcggc gcttaaattg gcgcgtcgtt atgcggtgga   4320

taattacggc tatcaaaaag ataccattat ttcattcaaa aaaagtttcc acggacgtac   4380

cttatttacc gtcagcgtcg gcggtcaggc gaaatattcg gacggattcg gcccgaaacc   4440

tgccggaatc gtgcatttgc cgtttaatga tcttgatgcg gttaaagcga tgattgatga   4500

tcacagctgt gcggtaatcg ttgagccgat tcagggagaa agcgggatta ttccggctac   4560

caaagaattt ttacagggtt tgcgtcgact ttgcgacgaa aataacgctc tattgatttt   4620

tgacgaagta caaacggggg tagggcgtac cggttattta tacgcatatg aaagttatga   4680

tgtagtgccg gatattctga cctcgtcaaa agcccttgct aacggttttc cgattagcgc   4740

aatgttaacc accacaaaaa ttgccgccag tttcaaaccg ggcgtgcacg gcaccacatt   4800

cggcggtaat ccgttagcct gtgcggtcgg ggcgaaagat ccaggcaaca cggggcagat   4860

cctgctttat cgcggcagtt cgttgcgtga atggaccttc gatcgcgtac tggcccacgc   4920

tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg gcgatcagca   4980

ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc gaaatcgctt   5040

tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac aatccgggca   5100

ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttt tagcgaagga   5160

tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc cctcaaaacg   5220

tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca atggcaaact   5280

tctacaacgc ccggtacacg aagctgagtc gttacgccag cagcatcaat ctgtctctcc   5340

ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg agattcagtt   5400

gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg gcactggaat   5460

gcggctgtat attgataacc aatctgagcg acttgttttg tggcggtatt acccacacga   5520

gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg ccctaaggat   5580

atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg tgatgagtag   5640

tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc acggagtggc   5700

tgtgctgcaa catggagcac tctggctact gggttaacat aatatcaggt ggaacaacgg   5760

atcaacagcg ggcaagggat ccgtttaaaa aatcagtcaa aaaagaccgc acttttaacg   5820

caaagttcgg tctttttttt gctatttttt ctccggtttt tccgtatcgg ctatttttaa   5880

ccggtaggaa aatattaatc ccaacaggct taaaacggat aaggtctgca aggcgatttg   5940

cagaccgtaa tgatctgcaa tccagcccac aattggtgaa aatatcccgc ccatagttat   6000

tcccagccca agggtaatgc cggctgcaaa gccgacgctt ttggcaagat aagcctgacc   6060

aagcacgaca ataggactat attgagtaaa aacgcccagt cccaacggaa ttagcaaaat   6120

aaatgagagc cataagtttt cgctttgagt aaaaattaaa atagtgggta aaaaaatcaa   6180

ataagcgtag cggataattc tgacataacc caggcgatcg gaaagcaacc cgccgataaa   6240

ggtaatggcg acccccattg ataaaaatat tgtgagcgca aagttagcgt cggtttcttg   6300

ttgatgaagt atatgaatcc aatagatagg aataaaagcg ttaagtactg tgaaattggt   6360

ggcgcggaca aaaataatca ccgacaattt ggcaaaactg cgccaatcgt tttgtaatgt   6420

ggtagaagcg gtgttttttg ctttattaac cacattttcg actgttaatt ttggcaactg   6480

taagaaaata atgagcgcga tgatcgtgtt aatcagggca aaaatactta aggtttgcgc   6540

accgaataaa taggcgagcc ccgcgaacat cggtcctata gcaaaaccgg cattgccgcc   6600

gacggcaaaa atccccatag ctttgccttt ttctccgccg gacattcggt taaccagtct   6660

cgctccttcc gggtgaaaca gagcggaacc tatgccggca atcatggcaa aaaagaataa   6720

acccgggtag gaatggacaa agcccatagc ggcaatacag cagcctgaca gcatcattcc   6780

caaaggaatt aaccaaggca tagaacgttt atccgccaaa tagccgaaaa acggttgagc   6840

aattgaggcg agaaccgtgt tggcgaaaat taatccgccg gcttcctgat aggtcaatcc   6900

gtaatttttt ataaaaagcg gaagcagggc ggggagcgct ccttgcgcca tatcggtgac   6960

gagatgcccg attgccgcaa gataaacagc gaatttgttt tgcataataa gttcgttaaa   7020

agataaataa gttctattat aatcccgaag tttccggtgt attttaataa aacgaattat   7080

tttgcatttt attacttaag atcaaaagac gggtatcggg aaattaaaag tgcggtgaaa   7140

atttgcgttt ttttggcggg tagaaaaaag ccctttcatc atgaaagggc aaaatacatt   7200

tggagttata ttttttaaag tatgtttgta ttatatatgc ggaattttaa cttttacaac   7260

atatttttaa catttacttt tggtgaggat tgcgcgtatt tctagactcc ataggccgct   7320

ttcctggctt tgcttccaga tgtatgctct cctccggaga gtaccgtgac tttattttcg   7380

gcacaaatac aggggtcgat ggataaatac ggcgatagtt tcctgacgga tgatccgtat   7440

gtaccggcgg aagacaagct gcaaacctgt cagatggaga ttgatttaat ggcggatgtg   7500

ctgagagcac cgccccgtga atccgcagaa ctgatccgct atgtgtttgc ggatgattgg   7560

ccggaataaa taaagccggg cttaatacag attaagcccg tatagggtat tattactgaa   7620

taccaaacag cttacggagg acggaatgtt acccattgag acaaccagac tgccttctga   7680

ttattaatat ttttcactat taatcagaag gaataaccat gaattttacc cggattgacc   7740

tgaatacctg gaatcgcagg gaacactttg ccctttatcg tcagcagatt aaatgcggat   7800

tcagcctgac caccaaactc gatattaccg ctttgcgtac cgcactggcg gagacaggtt   7860

ataagtttta tccgctgatg atttacctga tctcccgggc tgttaatcag tttccggagt   7920

tccggatggc actgaaagac aatgaactta tttactggga ccagtcagac ccggtcttta   7980

ctgtctttca taaagaaacc gaaacattct ctgcactgtc ctgccgttat tttccggatc   8040

tcagtgagtt tatggcaggt tataatgcgg taacggcaga atatcagcat gataccagat   8100


<210>  28
<211>  1779
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence of fusion-gene argD::cscA

<400>  28
atgagccaat atactcgcaa aacattcgat gaagtgatga tccaaaacta cgtacctgcg     60

gattttattc ctgtgaaagg caaaggttgc aaagtttggg atcagcaagg gcgagattat    120

attgatttta ccagcggaat tgccgtaaat gcattgggac attgtcctga cgaaatcgtc    180

gatgtgctga aaaaacaagg cgaaactcta tggcattcaa gcaactggtt caccagtgaa    240

ccgactctgg aacttgcctc caaattggtt gaacatacct ttgccgaacg tgtgatgttt    300

gccaattccg gcggtgaagc caatgaggcg gcgcttaaat tggcgcgtcg ttatgcggtg    360

gataattacg gctatcaaaa agataccatt atttcattca aaaaaagttt ccacggacgt    420

accttattta ccgtcagcgt cggcggtcag gcgaaatatt cggacggatt cggcccgaaa    480

cctgccggaa tcgtgcattt gccgtttaat gatcttgatg cggttaaagc gatgattgat    540

gatcacagct gtgcggtaat cgttgagccg attcagggag aaagcgggat tattccggct    600

accaaagaat ttttacaggg tttgcgtcga ctttgcgacg aaaataacgc tctattgatt    660

tttgacgaag tacaaacggg ggtagggcgt accggttatt tatacgcata tgaaagttat    720

gatgtagtgc cggatattct gacctcgtca aaagcccttg ctaacggttt tccgattagc    780

gcaatgttaa ccaccacaaa aattgccgcc agtttcaaac cgggcgtgca cggcaccaca    840

ttcggcggta atccgttagc ctgtgcggtc ggggcgaaag atccaggcaa cacggggcag    900

atcctgcttt atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac    960

gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag   1020

cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc   1080

tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg   1140

cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag   1200

gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa   1260

cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa   1320

cttctacaac gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct   1380

ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag   1440

ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga   1500

atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac   1560

gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg   1620

atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt   1680

agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg   1740

gctgtgctgc aacatggagc actctggcta ctgggttaa                          1779


<210>  29
<211>  5363
<212>  DNA
<213>  artificial sequence

<220>
<223>  complete nucleotide sequence of plasmid pSacB_pykA1

<400>  29
tcgagcagaa gattaagaag aacgaaaatc gtatgtacaa tggggcctgc aacagacaaa     60

ggcaataatt tagaaaaaat cattgctgcc ggtgcaaacg ttgtacgtat gaacttctcc    120

cacggtacgc ccgaagatca tatcggtcgt gctgaaaaag tacgtgaaat cgctcataaa    180

ttaggtaaac acgtagcaat cttaggtgac ttacaaggcc ctaaaatccg tgtttctact    240

tttaaagaag gcaaaatttt cttaaatatc ggtgataaat tcattttaga cgcagagatg    300

cctaaaggtg aaggtaacca ggaagcggtt ggtttagact ataaaacatt accgcaagat    360

gtggttccgg gcgatatctt attattagat gacggtcgag ttcaattgaa agtattggca    420

accgaaggtg caaaagtatt caccgaagta acggtcggtg gcccactatc aaataataaa    480

ggcattaaca aattaggctg cggtttatct gccgatgcat taaccgaaaa agataaagcg    540

gatatcatta ctgcggcgcg tatcggtgtg gattaccttg ccgtatcttt cccgcgttca    600

agcgcggatt taaactacgc ccgtcaatta gcaaaagatg cgggcttgga tgcgaaaatc    660

gttgcgaaag tagaacgtgc cgaaacagtt gaaacggacg aagcaatgga cgatatcatc    720

aatgcggcgg acgtaatcat ggttgcgcgc ggtgacttag gtgttgaaat cggtgatccg    780

gaattagtcg gtgttcagaa aaaattaatc cgtcgttcac gtcagttaaa tcgtgttgtt    840

attaccgcaa ctcaaatgat ggaatcaatg attagtaatc ctatgccgac tcgtgcggaa    900

gtaatggacg tagctaacgc agtattggac ggtaccgatg cggtaatgct ttctgctgaa    960

accgcggctg gtcaatatcc ggcggaaact gttgcggcga tggcgaaagt tgcgttaggt   1020

gcggagaaaa tgccaagcat taatgtgtct aaacaccgta tgaacgttca attcgagtct   1080

attgaagaat ctgttgcgat gtctgcaatg tatgcggcaa accacatgag aggcgtagcg   1140

gcgattatca cattaacaag tagcggtcgt actgctcgtt taatgtctag actccatagg   1200

ccgctttcct ggctttgctt ccagatgtat gctctcctcc ggagagtacc gtgactttat   1260

tttcggcaca aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc   1320

cgtatgtacc ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg   1380

atgtgctgag agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg   1440

attggccgga ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta   1500

ctgaatacca aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct   1560

tctgattatt aatatttttc actattaatc agaaggaata accatgaatt ttacccggat   1620

tgacctgaat acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg   1680

cggattcagc ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac   1740

aggttataag ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc   1800

ggagttccgg atggcactga aagacaatga acttatttac tgggaccagt cagacccggt   1860

ctttactgtc tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc   1920

ggatctcagt gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac   1980

cagattgttt ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg   2040

ggtgagtttt gacgggattt aacctgaaca tcaccggaaa tgatgattat tttgccccgg   2100

tttttacgat ggcaaagttt cagcaggaag gtgaccgcgt attattacct gtttctgtac   2160

aggttcatca tgcagtctgt gatggctttc atgcagcacg gtttattaat acacttcagc   2220

tgatgtgtga taacatactg aaataaatta attaattctg tatttaagcc accgtatccg   2280

gcaggaatgg tggctttttt tttatatttt aaccgtaatc tgtaatttcg tttcagactg   2340

gttcaggatg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca   2400

tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag   2460

cactagcggc gcgccggccg gcccggtgtg aaataccgca cagatgcgta aggagaaaat   2520

accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc   2580

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg   2640

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg   2700

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac   2760

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg   2820

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct   2880

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg   2940

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct   3000

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac   3060

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt   3120

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc   3180

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca   3240

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat   3300

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac   3360

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaagg   3420

ccggccgcgg ccgccatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc   3480

cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga   3540

agctcggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg   3600

taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta   3660

catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg   3720

ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt   3780

caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg ttcattttaa   3840

agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca   3900

cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact   3960

cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg   4020

tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag   4080

ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat   4140

ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat   4200

tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt   4260

tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt   4320

aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg   4380

ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt   4440

cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt   4500

tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa   4560

agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc   4620

tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac gaatcaaatt   4680

cagaaacttg atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg   4740

taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg   4800

cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg   4860

caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc   4920

ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta   4980

aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc   5040

ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc   5100

gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt   5160

gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt   5220

ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca gaaaatatca   5280

taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg ttaaaaagga   5340

tcggcggccg ctcgatttaa atc                                           5363


